AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73723

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73723.t1 MTR_7g077620 68.011 372 106 3 1 361 1 370 7.04e-174 489
MS.gene73723.t1 MTR_7g077650 65.263 380 107 10 1 363 1 372 1.16e-154 439
MS.gene73723.t1 MTR_7g077660 62.334 377 119 10 1 363 1 368 9.37e-146 417
MS.gene73723.t1 MTR_1g108740 44.205 371 170 15 1 361 1 344 2.99e-85 262
MS.gene73723.t1 MTR_7g076110 49.831 295 127 6 87 363 22 313 3.48e-82 253
MS.gene73723.t1 MTR_7g076100 58.475 236 86 3 139 363 66 300 1.58e-80 248
MS.gene73723.t1 MTR_7g076100 78.182 55 10 2 1 54 1 54 7.75e-19 86.3
MS.gene73723.t1 MTR_5g038260 40.351 399 181 15 3 363 4 383 4.95e-74 234
MS.gene73723.t1 MTR_5g038230 40.568 387 163 15 5 361 4 353 2.27e-70 224
MS.gene73723.t1 MTR_1g064570 39.379 419 176 16 3 363 2 400 3.55e-70 225
MS.gene73723.t1 MTR_5g082770 44.590 305 136 9 5 296 4 288 2.03e-65 209
MS.gene73723.t1 MTR_1g016150 45.652 230 107 7 1 216 1 226 1.83e-52 174
MS.gene73723.t1 MTR_5g082780 39.035 228 115 8 115 328 1 218 1.30e-31 119
MS.gene73723.t1 MTR_7g076130 50.769 130 49 5 169 284 25 153 4.02e-26 102
MS.gene73723.t1 MTR_1g108750 43.949 157 51 6 5 160 4 124 2.11e-21 89.0
MS.gene73723.t1 MTR_3g114970 24.148 352 200 11 4 324 3 318 3.42e-17 82.0
MS.gene73723.t1 MTR_3g114990 26.110 383 209 16 4 340 22 376 3.71e-17 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTCCATCCTTCACATTA+TGG 0.195674 6.3:+34920402 None:intergenic
CAAACTCTACGTTTACAAAT+TGG 0.205980 6.3:-34920606 MS.gene73723:CDS
TTTCAACATACTTGAGAGTT+TGG 0.271304 6.3:-34920552 MS.gene73723:CDS
CAACCGGATGTCACATCTTT+GGG 0.283463 6.3:-34920351 MS.gene73723:CDS
GTTGAACCCGAAGCTTAAAA+TGG 0.296182 6.3:+34920775 None:intergenic
TCAACCGGATGTCACATCTT+TGG 0.300744 6.3:-34920352 MS.gene73723:CDS
TAATTACTTGGGCTTGTAAT+CGG 0.303729 6.3:+34920944 None:intergenic
GGAGCTACACAATACTTAAA+AGG 0.322226 6.3:+34921019 None:intergenic
GACATCCGGTTGAAGGAAAT+TGG 0.322776 6.3:+34920362 None:intergenic
TGGATTCGTGTAAACAGATA+TGG 0.324446 6.3:-34920860 MS.gene73723:CDS
CAATGAAATGACGGTGTGTT+TGG 0.335412 6.3:-34921101 MS.gene73723:CDS
AAGAACACATAGATCATGTT+AGG 0.348741 6.3:-34921330 MS.gene73723:CDS
AAAAGGATTCGCTAGTGTTA+TGG 0.360726 6.3:+34921036 None:intergenic
GAAGGATGGGATAATTGAAT+AGG 0.370358 6.3:+34921127 None:intergenic
AGAAGGTCTACCTCTTAGTT+TGG 0.383875 6.3:-34921368 MS.gene73723:CDS
CTACGTTTACAAATTGGAAC+TGG 0.389715 6.3:-34920600 MS.gene73723:CDS
CTTTACCAATTTCCTTCAAC+CGG 0.393860 6.3:-34920367 MS.gene73723:CDS
TTTCCAGAACAATATCATTA+CGG 0.401041 6.3:-34920713 MS.gene73723:CDS
CCATGGTTGGCTTGCATTGG+TGG 0.402911 6.3:-34921071 MS.gene73723:CDS
TTGGAGAGGTGGCAGACTAA+TGG 0.403671 6.3:+34920997 None:intergenic
GGGTTCTACCACAATTTCAA+TGG 0.406178 6.3:-34920331 MS.gene73723:CDS
AGGCAATGAGGAAGGTAGAT+TGG 0.421191 6.3:-34921399 None:intergenic
AAAGAGCAAGTCAATCATCT+TGG 0.426179 6.3:-34920880 MS.gene73723:CDS
TGGAGAGGTGGCAGACTAAT+GGG 0.429349 6.3:+34920998 None:intergenic
CCTCCGTAATGATATTGTTC+TGG 0.431440 6.3:+34920710 None:intergenic
GTAGATTGGGCAAATTTAGA+AGG 0.435773 6.3:-34921385 MS.gene73723:CDS
AATTACTTGGGCTTGTAATC+GGG 0.438323 6.3:+34920945 None:intergenic
GCTGCAACCACATAATTACT+TGG 0.439750 6.3:+34920932 None:intergenic
CATTTCATTGATTGAAGGAT+GGG 0.455943 6.3:+34921114 None:intergenic
AAGGATGGGATAATTGAATA+GGG 0.463451 6.3:+34921128 None:intergenic
AATGAAATGACGGTGTGTTT+GGG 0.468609 6.3:-34921100 MS.gene73723:CDS
GGCAATGAGGAAGGTAGATT+GGG 0.471328 6.3:-34921398 None:intergenic
TAGTTTGGTTCTTGAGAAGT+TGG 0.473111 6.3:-34921353 MS.gene73723:CDS
GGTTCTACCACAATTTCAAT+GGG 0.480142 6.3:-34920330 MS.gene73723:CDS
TAGCCATGGTTGGCTTGCAT+TGG 0.484269 6.3:-34921074 MS.gene73723:CDS
AATCAATGACCGGAGACATA+TGG 0.484616 6.3:-34920670 MS.gene73723:CDS
TCATTTCATTGATTGAAGGA+TGG 0.486040 6.3:+34921113 None:intergenic
ACCTCTCCAATCTGTTTACA+AGG 0.490029 6.3:-34920984 MS.gene73723:CDS
ACCGTCATTTCATTGATTGA+AGG 0.491455 6.3:+34921109 None:intergenic
TTCTCAAGAACCAAACTAAG+AGG 0.491952 6.3:+34921358 None:intergenic
CTGATATTTGCTGTTGGCAA+CGG 0.495311 6.3:-34920803 MS.gene73723:CDS
CAATTCTGCTTGTTATGAGA+TGG 0.510153 6.3:-34920753 MS.gene73723:CDS
AAGATGTGACATCCGGTTGA+AGG 0.515408 6.3:+34920355 None:intergenic
TACAAGCCCAAGTAATTATG+TGG 0.515745 6.3:-34920939 MS.gene73723:CDS
CAAATCCATAATGTGAAGGA+TGG 0.524725 6.3:-34920407 MS.gene73723:CDS
TTTCACCATCCATATGTCTC+CGG 0.527974 6.3:+34920661 None:intergenic
GATCAATCCCATTGAAATTG+TGG 0.531759 6.3:+34920323 None:intergenic
GTGTGTTTGGGCGTTAGCCA+TGG 0.536708 6.3:-34921088 MS.gene73723:CDS
AAAGGTCTGATATTTGCTGT+TGG 0.547450 6.3:-34920809 MS.gene73723:CDS
GTTTGGGCGTTAGCCATGGT+TGG 0.554659 6.3:-34921084 MS.gene73723:CDS
AGGTAACCTTGTAAACAGAT+TGG 0.555212 6.3:+34920978 None:intergenic
GAACCCAAAGATGTGACATC+CGG 0.561035 6.3:+34920348 None:intergenic
TTCGTGTGGTGTGCAAGACT+TGG 0.561436 6.3:-34921306 MS.gene73723:CDS
CTGCAACCACATAATTACTT+GGG 0.562894 6.3:+34920933 None:intergenic
GAGAGGATCATCAACATAGG+AGG 0.569522 6.3:+34921229 None:intergenic
CAGAACAATATCATTACGGA+GGG 0.584738 6.3:-34920709 MS.gene73723:CDS
TATGAGAATTATTGTATGAG+AGG 0.586159 6.3:+34921212 None:intergenic
TCCTTCAATCAATGAAATGA+CGG 0.587156 6.3:-34921110 MS.gene73723:CDS
ATTATGATAGGCAATGAGGA+AGG 0.591409 6.3:-34921407 None:intergenic
GTTGCAGCGATTTACAATTG+TGG 0.603650 6.3:-34920917 MS.gene73723:CDS
TTGTAAACAGATTGGAGAGG+TGG 0.610040 6.3:+34920986 None:intergenic
GTTACAAATCCATAATGTGA+AGG 0.610111 6.3:-34920411 MS.gene73723:CDS
AGAGGATCATCAACATAGGA+GGG 0.610715 6.3:+34921230 None:intergenic
TTGGAACTGGACCCTATGAG+TGG 0.614160 6.3:-34920587 MS.gene73723:CDS
TATGAGAGGATCATCAACAT+AGG 0.617496 6.3:+34921226 None:intergenic
CCAGAACAATATCATTACGG+AGG 0.624405 6.3:-34920710 MS.gene73723:CDS
AGTTTGCATAAATGCTTGCG+AGG 0.629904 6.3:+34920623 None:intergenic
AGATCATGTTAGGTTTCGTG+TGG 0.631367 6.3:-34921320 MS.gene73723:CDS
AAATTGGTAAAGATTGACTA+CGG 0.637052 6.3:+34920378 None:intergenic
TGTAATCGGGTCATCAGACA+AGG 0.645501 6.3:+34920958 None:intergenic
CCACCAATGCAAGCCAACCA+TGG 0.665097 6.3:+34921071 None:intergenic
AATGACCGGAGACATATGGA+TGG 0.696827 6.3:-34920666 MS.gene73723:CDS
ACCTTGTAAACAGATTGGAG+AGG 0.742777 6.3:+34920983 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTATTTTATTTATGA+TGG - chr6.3:34921267-34921286 MS.gene73723:CDS 10.0%
!! ACCATAAAAATCATCAATTT+AGG - chr6.3:34920458-34920477 MS.gene73723:CDS 20.0%
!!! AACATTTTATTTGTTGGAAT+CGG - chr6.3:34921195-34921214 MS.gene73723:CDS 20.0%
!!! ACTGATGTTGTATTTTATAA+AGG - chr6.3:34920892-34920911 MS.gene73723:CDS 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG + chr6.3:34920462-34920481 None:intergenic 20.0%
! AAATTGGTAAAGATTGACTA+CGG + chr6.3:34921344-34921363 None:intergenic 25.0%
! TATGAGAATTATTGTATGAG+AGG + chr6.3:34920510-34920529 None:intergenic 25.0%
! TTTCCAGAACAATATCATTA+CGG - chr6.3:34921006-34921025 MS.gene73723:CDS 25.0%
!! AATTGAATCCTTTTCAAACT+TGG + chr6.3:34921253-34921272 None:intergenic 25.0%
!! TATTTTGTGAAATCAATGAC+CGG - chr6.3:34921039-34921058 MS.gene73723:CDS 25.0%
!!! GAAAGCAACATTTTATTTGT+TGG - chr6.3:34921189-34921208 MS.gene73723:CDS 25.0%
!!! TATTTTATTTATGATGGCGA+CGG - chr6.3:34921273-34921292 MS.gene73723:CDS 25.0%
!!! TTGTTTTTCCAAGTTTGAAA+AGG - chr6.3:34921242-34921261 MS.gene73723:CDS 25.0%
AAGAACACATAGATCATGTT+AGG - chr6.3:34920389-34920408 MS.gene73723:CDS 30.0%
AAGGATGGGATAATTGAATA+GGG + chr6.3:34920594-34920613 None:intergenic 30.0%
CAAACTCTACGTTTACAAAT+TGG - chr6.3:34921113-34921132 MS.gene73723:CDS 30.0%
GTTACAAATCCATAATGTGA+AGG - chr6.3:34921308-34921327 MS.gene73723:CDS 30.0%
TAATTACTTGGGCTTGTAAT+CGG + chr6.3:34920778-34920797 None:intergenic 30.0%
TCCTTCAATCAATGAAATGA+CGG - chr6.3:34920609-34920628 MS.gene73723:CDS 30.0%
TTCAAACTTGGAAAAACAAG+AGG + chr6.3:34921241-34921260 None:intergenic 30.0%
TTTCAACATACTTGAGAGTT+TGG - chr6.3:34921167-34921186 MS.gene73723:CDS 30.0%
! CATTTCATTGATTGAAGGAT+GGG + chr6.3:34920608-34920627 None:intergenic 30.0%
! TCATTTCATTGATTGAAGGA+TGG + chr6.3:34920609-34920628 None:intergenic 30.0%
!! GTTTAACTTTTCCACTCATA+GGG + chr6.3:34921146-34921165 None:intergenic 30.0%
!! TGTTTAACTTTTCCACTCAT+AGG + chr6.3:34921147-34921166 None:intergenic 30.0%
AAAAGGATTCGCTAGTGTTA+TGG + chr6.3:34920686-34920705 None:intergenic 35.0%
AAAGAGCAAGTCAATCATCT+TGG - chr6.3:34920839-34920858 MS.gene73723:CDS 35.0%
AACTTCCATCCTTCACATTA+TGG + chr6.3:34921320-34921339 None:intergenic 35.0%
AATGAAATGACGGTGTGTTT+GGG - chr6.3:34920619-34920638 MS.gene73723:CDS 35.0%
AATTACTTGGGCTTGTAATC+GGG + chr6.3:34920777-34920796 None:intergenic 35.0%
AGGTAACCTTGTAAACAGAT+TGG + chr6.3:34920744-34920763 None:intergenic 35.0%
CAAATCCATAATGTGAAGGA+TGG - chr6.3:34921312-34921331 MS.gene73723:CDS 35.0%
CAGAACAATATCATTACGGA+GGG - chr6.3:34921010-34921029 MS.gene73723:CDS 35.0%
CTACGTTTACAAATTGGAAC+TGG - chr6.3:34921119-34921138 MS.gene73723:CDS 35.0%
CTGCAACCACATAATTACTT+GGG + chr6.3:34920789-34920808 None:intergenic 35.0%
CTTTACCAATTTCCTTCAAC+CGG - chr6.3:34921352-34921371 MS.gene73723:CDS 35.0%
GAAGGATGGGATAATTGAAT+AGG + chr6.3:34920595-34920614 None:intergenic 35.0%
GGAGCTACACAATACTTAAA+AGG + chr6.3:34920703-34920722 None:intergenic 35.0%
GGTTCTACCACAATTTCAAT+GGG - chr6.3:34921389-34921408 MS.gene73723:CDS 35.0%
GTAGATTGGGCAAATTTAGA+AGG - chr6.3:34920334-34920353 MS.gene73723:CDS 35.0%
TACAAGCCCAAGTAATTATG+TGG - chr6.3:34920780-34920799 MS.gene73723:CDS 35.0%
TATGAGAGGATCATCAACAT+AGG + chr6.3:34920496-34920515 None:intergenic 35.0%
TGGATTCGTGTAAACAGATA+TGG - chr6.3:34920859-34920878 MS.gene73723:CDS 35.0%
TTCTCAAGAACCAAACTAAG+AGG + chr6.3:34920364-34920383 None:intergenic 35.0%
! ACCGTCATTTCATTGATTGA+AGG + chr6.3:34920613-34920632 None:intergenic 35.0%
!! AAAGGTCTGATATTTGCTGT+TGG - chr6.3:34920910-34920929 MS.gene73723:CDS 35.0%
!! CAATTCTGCTTGTTATGAGA+TGG - chr6.3:34920966-34920985 MS.gene73723:CDS 35.0%
!! TAGTTTGGTTCTTGAGAAGT+TGG - chr6.3:34920366-34920385 MS.gene73723:CDS 35.0%
AACTTGGAAAAACAAGAGGC+TGG + chr6.3:34921237-34921256 None:intergenic 40.0%
AATCAATGACCGGAGACATA+TGG - chr6.3:34921049-34921068 MS.gene73723:CDS 40.0%
ACCTCTCCAATCTGTTTACA+AGG - chr6.3:34920735-34920754 MS.gene73723:CDS 40.0%
ACCTTGTAAACAGATTGGAG+AGG + chr6.3:34920739-34920758 None:intergenic 40.0%
AGAAGGTCTACCTCTTAGTT+TGG - chr6.3:34920351-34920370 MS.gene73723:CDS 40.0%
AGAGGATCATCAACATAGGA+GGG + chr6.3:34920492-34920511 None:intergenic 40.0%
AGATCATGTTAGGTTTCGTG+TGG - chr6.3:34920399-34920418 MS.gene73723:CDS 40.0%
AGTTTGCATAAATGCTTGCG+AGG + chr6.3:34921099-34921118 None:intergenic 40.0%
CAATGAAATGACGGTGTGTT+TGG - chr6.3:34920618-34920637 MS.gene73723:CDS 40.0%
CCAGAACAATATCATTACGG+AGG - chr6.3:34921009-34921028 MS.gene73723:CDS 40.0%
CCTCCGTAATGATATTGTTC+TGG + chr6.3:34921012-34921031 None:intergenic 40.0%
GCTGCAACCACATAATTACT+TGG + chr6.3:34920790-34920809 None:intergenic 40.0%
GGGTTCTACCACAATTTCAA+TGG - chr6.3:34921388-34921407 MS.gene73723:CDS 40.0%
GTTGAACCCGAAGCTTAAAA+TGG + chr6.3:34920947-34920966 None:intergenic 40.0%
GTTGCAGCGATTTACAATTG+TGG - chr6.3:34920802-34920821 MS.gene73723:CDS 40.0%
TTGTAAACAGATTGGAGAGG+TGG + chr6.3:34920736-34920755 None:intergenic 40.0%
TTTCACCATCCATATGTCTC+CGG + chr6.3:34921061-34921080 None:intergenic 40.0%
! GAGACACCATTTTAAGCTTC+GGG - chr6.3:34920938-34920957 MS.gene73723:CDS 40.0%
! GGAGACACCATTTTAAGCTT+CGG - chr6.3:34920937-34920956 MS.gene73723:CDS 40.0%
!! CTGATATTTGCTGTTGGCAA+CGG - chr6.3:34920916-34920935 MS.gene73723:CDS 40.0%
AAGATGTGACATCCGGTTGA+AGG + chr6.3:34921367-34921386 None:intergenic 45.0%
AATGACCGGAGACATATGGA+TGG - chr6.3:34921053-34921072 MS.gene73723:CDS 45.0%
CAACCGGATGTCACATCTTT+GGG - chr6.3:34921368-34921387 MS.gene73723:CDS 45.0%
GAACCCAAAGATGTGACATC+CGG + chr6.3:34921374-34921393 None:intergenic 45.0%
GACATCCGGTTGAAGGAAAT+TGG + chr6.3:34921360-34921379 None:intergenic 45.0%
GAGAGGATCATCAACATAGG+AGG + chr6.3:34920493-34920512 None:intergenic 45.0%
TCAACCGGATGTCACATCTT+TGG - chr6.3:34921367-34921386 MS.gene73723:CDS 45.0%
TGTAATCGGGTCATCAGACA+AGG + chr6.3:34920764-34920783 None:intergenic 45.0%
TGGAGAGGTGGCAGACTAAT+GGG + chr6.3:34920724-34920743 None:intergenic 50.0%
TTCGTGTGGTGTGCAAGACT+TGG - chr6.3:34920413-34920432 MS.gene73723:CDS 50.0%
TTGGAACTGGACCCTATGAG+TGG - chr6.3:34921132-34921151 MS.gene73723:CDS 50.0%
TTGGAGAGGTGGCAGACTAA+TGG + chr6.3:34920725-34920744 None:intergenic 50.0%
!! TAGCCATGGTTGGCTTGCAT+TGG - chr6.3:34920645-34920664 MS.gene73723:CDS 50.0%
CCACCAATGCAAGCCAACCA+TGG + chr6.3:34920651-34920670 None:intergenic 55.0%
GTGTGTTTGGGCGTTAGCCA+TGG - chr6.3:34920631-34920650 MS.gene73723:CDS 55.0%
GTTTGGGCGTTAGCCATGGT+TGG - chr6.3:34920635-34920654 MS.gene73723:CDS 55.0%
!! CCATGGTTGGCTTGCATTGG+TGG - chr6.3:34920648-34920667 MS.gene73723:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 34920325 34921416 34920325 ID=MS.gene73723
chr6.3 mRNA 34920325 34921416 34920325 ID=MS.gene73723.t1;Parent=MS.gene73723
chr6.3 exon 34920325 34921416 34920325 ID=MS.gene73723.t1.exon1;Parent=MS.gene73723.t1
chr6.3 CDS 34920325 34921416 34920325 ID=cds.MS.gene73723.t1;Parent=MS.gene73723.t1
Gene Sequence

>MS.gene73723

ATGAGGAAGGTAGATTGGGCAAATTTAGAAGGTCTACCTCTTAGTTTGGTTCTTGAGAAGTTGGAAGAACACATAGATCATGTTAGGTTTCGTGTGGTGTGCAAGACTTGGCTATTAGTTGCAAATCATCTTAACCATAAAAATCATCAATTTAGGATTAATGTCCCTCCTATGTTGATGATCCTCTCATACAATAATTCTCATATACGTACAATATCACAGACGAAGAAAAGATCTTTGTATAGTATTCTTAGTGAGAAAGAGTACCCTATTCAATTATCCCATCCTTCAATCAATGAAATGACGGTGTGTTTGGGCGTTAGCCATGGTTGGCTTGCATTGGTGGATGACAACAAAGCCATAACACTAGCGAATCCTTTTAAGTATTGTGTAGCTCCCATTAGTCTGCCACCTCTCCAATCTGTTTACAAGGTTACCTTGTCTGATGACCCGATTACAAGCCCAAGTAATTATGTGGTTGCAGCGATTTACAATTGTGGTTCTCTCGCTTTCAAAAGAGCAAGTCAATCATCTTGGATTCGTGTAAACAGATATGGATTTTATATCACTGATGTTGTATTTTATAAAGGTCTGATATTTGCTGTTGGCAACGGAGACACCATTTTAAGCTTCGGGTTCAACAATTCTGCTTGTTATGAGATGGCTAGTACTCAGAATTTTTTTCCAGAACAATATCATTACGGAGGGCATGCTTATTTTGTGAAATCAATGACCGGAGACATATGGATGGTGAAAAGATGTTTAATTGATCCTCGCAAGCATTTATGCAAACTCTACGTTTACAAATTGGAACTGGACCCTATGAGTGGAAAAGTTAAACATTTCAACATACTTGAGAGTTTGGAAAGCAACATTTTATTTGTTGGAATCGGAGATTCAATTTCTGTGCCAGCCTCTTGTTTTTCCAAGTTTGAAAAGGATTCAATTTATTTTATTTATGATGGCGACGGAGATGATTTAGAGTTACAAATCCATAATGTGAAGGATGGAAGTTTCCGTAGTCAATCTTTACCAATTTCCTTCAACCGGATGTCACATCTTTGGGTTCTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene73723.t1

MRKVDWANLEGLPLSLVLEKLEEHIDHVRFRVVCKTWLLVANHLNHKNHQFRINVPPMLMILSYNNSHIRTISQTKKRSLYSILSEKEYPIQLSHPSINEMTVCLGVSHGWLALVDDNKAITLANPFKYCVAPISLPPLQSVYKVTLSDDPITSPSNYVVAAIYNCGSLAFKRASQSSWIRVNRYGFYITDVVFYKGLIFAVGNGDTILSFGFNNSACYEMASTQNFFPEQYHYGGHAYFVKSMTGDIWMVKRCLIDPRKHLCKLYVYKLELDPMSGKVKHFNILESLESNILFVGIGDSISVPASCFSKFEKDSIYFIYDGDGDDLELQIHNVKDGSFRSQSLPISFNRMSHLWVLPQFQWD