AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94237


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94237.t1 MTR_5g082780 91.342 231 20 0 98 328 1 231 3.71e-155 435
MS.gene94237.t1 MTR_5g082770 64.505 293 90 6 1 283 1 289 2.03e-120 349
MS.gene94237.t1 MTR_5g038260 45.692 383 180 10 1 359 3 381 1.27e-101 305
MS.gene94237.t1 MTR_1g064570 44.279 402 177 10 1 359 1 398 3.12e-101 305
MS.gene94237.t1 MTR_5g038230 46.257 374 165 10 1 359 1 353 4.06e-99 298
MS.gene94237.t1 MTR_1g108740 43.164 373 162 10 4 359 5 344 1.61e-85 262
MS.gene94237.t1 MTR_7g077620 41.919 396 164 14 2 359 3 370 8.12e-76 239
MS.gene94237.t1 MTR_7g077660 42.132 394 160 18 3 359 4 366 2.19e-75 237
MS.gene94237.t1 MTR_7g077650 40.657 396 167 18 2 358 3 369 1.06e-72 230
MS.gene94237.t1 MTR_1g016150 44.737 190 68 6 4 171 5 179 2.92e-41 145
MS.gene94237.t1 MTR_1g016150 48.718 78 31 1 219 296 168 236 2.13e-14 72.4
MS.gene94237.t1 MTR_7g076100 40.408 245 112 9 133 359 70 298 1.49e-38 139
MS.gene94237.t1 MTR_7g076110 35.161 310 158 11 73 359 22 311 3.09e-38 139
MS.gene94237.t1 MTR_1g108750 45.033 151 55 3 1 150 1 124 7.04e-27 103
MS.gene94237.t1 MTR_3g114990 25.123 406 238 16 1 357 20 408 5.30e-18 85.1
MS.gene94237.t1 MTR_3g114970 26.329 395 211 23 1 357 1 353 4.18e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94237.t1 AT2G17036 23.618 398 243 16 3 354 1 383 1.02e-12 69.3
MS.gene94237.t1 AT2G17036 23.618 398 243 16 3 354 2 384 1.08e-12 69.3

Find 70 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAATCTCATTGGAAATT+TGG 0.118149 5.4:+65493986 None:intergenic
TAGCATTTGTTTAAACTTTC+CGG 0.160572 5.4:+65494267 None:intergenic
GGAACTAGATTGGGCAAATT+TGG 0.170985 5.4:-65495049 MS.gene94237:CDS
TAAGAATTTGGCAATTTAAA+TGG 0.183830 5.4:+65494826 None:intergenic
TAATCATTTGGTCTTGTTAT+AGG 0.187600 5.4:+65494631 None:intergenic
TACAAACATCTTTCTGTTCT+TGG 0.199967 5.4:+65494874 None:intergenic
AGCAGCAGCCACCACCTTTC+TGG 0.214961 5.4:-65494015 MS.gene94237:CDS
TTTATGGATGTTATGTTCTA+TGG 0.218295 5.4:-65494505 MS.gene94237:CDS
CAAATATGGTAATAAGAATT+TGG 0.251801 5.4:+65494814 None:intergenic
GAGCTGGCATCATTCCTTTA+TGG 0.256715 5.4:-65494521 MS.gene94237:CDS
CAAATAGATCACATTTGTTT+TGG 0.284203 5.4:-65494985 MS.gene94237:CDS
GGCTAAATCCACAACAAATA+TGG 0.289458 5.4:+65494800 None:intergenic
AATCATTTGGTCTTGTTATA+GGG 0.294407 5.4:+65494632 None:intergenic
GATAACTCCAAATCCATTTC+AGG 0.313372 5.4:-65494419 MS.gene94237:CDS
AACAAGACCAAATGATTATA+TGG 0.314220 5.4:-65494626 MS.gene94237:CDS
ACTTGTGGTCCATTTCATAT+AGG 0.341950 5.4:-65494100 MS.gene94237:CDS
TCGAGGGTTGGAAGACTAAT+AGG 0.372920 5.4:+65494709 None:intergenic
GCTATGTCCTGAAATGGATT+TGG 0.374010 5.4:+65494412 None:intergenic
ACAAACATCTTTCTGTTCTT+GGG 0.381418 5.4:+65494875 None:intergenic
TGAAAAGCTATGTCCTGAAA+TGG 0.396599 5.4:+65494406 None:intergenic
GCAGCAGCCACCACCTTTCT+GGG 0.398247 5.4:-65494014 MS.gene94237:CDS
GCCGCAACCATATAATCATT+TGG 0.404338 5.4:+65494619 None:intergenic
TGGTTAGAATGGAACTAGAT+TGG 0.415806 5.4:-65495059 None:intergenic
TGATTTAATTCTTGATAAGT+TGG 0.416195 5.4:-65495013 MS.gene94237:CDS
CTTGTGGTCCATTTCATATA+GGG 0.417934 5.4:-65494099 MS.gene94237:CDS
GGTTAGAATGGAACTAGATT+GGG 0.421888 5.4:-65495058 None:intergenic
GCAATTCACACTAGCATTCA+TGG 0.422023 5.4:-65494598 MS.gene94237:CDS
TTATATATCCCTATATGAAA+TGG 0.424795 5.4:+65494091 None:intergenic
CGAATTCAAACTTGTACACT+TGG 0.434747 5.4:+65494299 None:intergenic
TTATCTTGTGAAATCATTAG+AGG 0.436221 5.4:-65494368 MS.gene94237:CDS
ATAGGAGCAACATTCTTAGA+AGG 0.444870 5.4:+65494727 None:intergenic
ATAGAACATAACATCCATAA+AGG 0.458967 5.4:+65494507 None:intergenic
AGAAGGATGCAATAGTGTTA+TGG 0.460688 5.4:+65494744 None:intergenic
TGTTCTTGGGATCATTAGCA+TGG 0.462460 5.4:+65494888 None:intergenic
ATGCTAAAGCTTGAGAGTCT+TGG 0.465110 5.4:-65494250 MS.gene94237:CDS
TAAAAGCGGGCGAACCATGC+TGG 0.470666 5.4:-65494561 MS.gene94237:CDS
GTATCAGCTTCATACTTCTC+CGG 0.473456 5.4:-65494187 MS.gene94237:CDS
TTGGTGCCGTGTGCAAGAAT+TGG 0.477626 5.4:-65494966 MS.gene94237:CDS
AACCATGCTGGACTTATGTA+TGG 0.478737 5.4:-65494549 MS.gene94237:CDS
TCTTATTACCATATTTGTTG+TGG 0.483010 5.4:-65494808 MS.gene94237:CDS
TTTATCTGAGGTCGGGTACA+AGG 0.517119 5.4:-65494671 MS.gene94237:CDS
ACCAAATGATTATATGGTTG+CGG 0.517425 5.4:-65494620 MS.gene94237:CDS
GTTGTGGATTTAGCCATGGT+TGG 0.523421 5.4:-65494792 MS.gene94237:CDS
TAAAGGAATGATGCCAGCTC+GGG 0.530279 5.4:+65494524 None:intergenic
ATACAAGTTTATCTGAGGTC+GGG 0.533775 5.4:-65494678 MS.gene94237:CDS
GGGATATATAATGTAAGAGA+TGG 0.535003 5.4:-65494079 MS.gene94237:CDS
CTTATGTATGGATCCCGAGC+TGG 0.538699 5.4:-65494537 MS.gene94237:CDS
TATACAAGTTTATCTGAGGT+CGG 0.543905 5.4:-65494679 MS.gene94237:CDS
ATTTGTTGTGGATTTAGCCA+TGG 0.546254 5.4:-65494796 MS.gene94237:CDS
ATGGATGTTATGTTCTATGG+AGG 0.548376 5.4:-65494502 MS.gene94237:CDS
AAATTTGGTAAAACCCAGAA+AGG 0.548520 5.4:+65494001 None:intergenic
GTTCTTGGGATCATTAGCAT+GGG 0.554796 5.4:+65494889 None:intergenic
TTTGAATTCGATGCTCAGAC+CGG 0.560810 5.4:-65494286 MS.gene94237:CDS
TATCTTGTGAAATCATTAGA+GGG 0.568311 5.4:-65494367 MS.gene94237:CDS
ATAAAGGAATGATGCCAGCT+CGG 0.569621 5.4:+65494523 None:intergenic
GTGAAGATTATGATGATCAG+AGG 0.588224 5.4:+65494054 None:intergenic
GGTAAAACCCAGAAAGGTGG+TGG 0.590899 5.4:+65494007 None:intergenic
CTATTGTTGCAAGCCAACCA+TGG 0.611822 5.4:+65494779 None:intergenic
AACTTGTATATGATCGATCG+AGG 0.611957 5.4:+65494692 None:intergenic
AAGTTAGATTGTGTAACTTG+TGG 0.613074 5.4:-65494115 MS.gene94237:CDS
ATCATATACAAGTTTATCTG+AGG 0.615631 5.4:-65494683 MS.gene94237:CDS
GTATATGATCGATCGAGGGT+TGG 0.616455 5.4:+65494697 None:intergenic
AGCTTAACCATCAAAATCAG+CGG 0.617597 5.4:-65494930 MS.gene94237:CDS
AATCATTAGAGGGAGACTTG+TGG 0.640892 5.4:-65494357 MS.gene94237:CDS
TTTGGTAAAACCCAGAAAGG+TGG 0.643098 5.4:+65494004 None:intergenic
TAATCGCCAATTCTTGCACA+CGG 0.645036 5.4:+65494960 None:intergenic
ATCCATACATAAGTCCAGCA+TGG 0.649735 5.4:+65494547 None:intergenic
TATGTTGCGAGTAAATATCA+CGG 0.663367 5.4:-65494472 MS.gene94237:CDS
ACTTGTATATGATCGATCGA+GGG 0.674359 5.4:+65494693 None:intergenic
ATTAGAGGGAGACTTGTGGA+TGG 0.690554 5.4:-65494353 MS.gene94237:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATGGATACATTTTTT+TGG + chr5.4:65494208-65494227 None:intergenic 15.0%
!! CAAATATGGTAATAAGAATT+TGG + chr5.4:65494234-65494253 None:intergenic 20.0%
!! TAAGAATTTGGCAATTTAAA+TGG + chr5.4:65494222-65494241 None:intergenic 20.0%
!! TGATTTAATTCTTGATAAGT+TGG - chr5.4:65494032-65494051 MS.gene94237:CDS 20.0%
!! TTATATATCCCTATATGAAA+TGG + chr5.4:65494957-65494976 None:intergenic 20.0%
! AACAAGACCAAATGATTATA+TGG - chr5.4:65494419-65494438 MS.gene94237:CDS 25.0%
! ATAGAACATAACATCCATAA+AGG + chr5.4:65494541-65494560 None:intergenic 25.0%
! ATCATATACAAGTTTATCTG+AGG - chr5.4:65494362-65494381 MS.gene94237:CDS 25.0%
! TAGCATTTGTTTAAACTTTC+CGG + chr5.4:65494781-65494800 None:intergenic 25.0%
! TATCTTGTGAAATCATTAGA+GGG - chr5.4:65494678-65494697 MS.gene94237:CDS 25.0%
! TTATCTTGTGAAATCATTAG+AGG - chr5.4:65494677-65494696 MS.gene94237:CDS 25.0%
! TTTATGGATGTTATGTTCTA+TGG - chr5.4:65494540-65494559 MS.gene94237:CDS 25.0%
!! ACGTTTCGATTTTATAAAAG+CGG - chr5.4:65494470-65494489 MS.gene94237:CDS 25.0%
!! CAAATAGATCACATTTGTTT+TGG - chr5.4:65494060-65494079 MS.gene94237:CDS 25.0%
!! GGTATAGTATCTTTTAATCT+TGG - chr5.4:65494594-65494613 MS.gene94237:CDS 25.0%
!! TCTTATTACCATATTTGTTG+TGG - chr5.4:65494237-65494256 MS.gene94237:CDS 25.0%
!!! AATCATTTGGTCTTGTTATA+GGG + chr5.4:65494416-65494435 None:intergenic 25.0%
!!! TAATCATTTGGTCTTGTTAT+AGG + chr5.4:65494417-65494436 None:intergenic 25.0%
AAATTTGGTAAAACCCAGAA+AGG + chr5.4:65495047-65495066 None:intergenic 30.0%
AAGTTAGATTGTGTAACTTG+TGG - chr5.4:65494930-65494949 MS.gene94237:CDS 30.0%
ACAAACATCTTTCTGTTCTT+GGG + chr5.4:65494173-65494192 None:intergenic 30.0%
ACCAAATGATTATATGGTTG+CGG - chr5.4:65494425-65494444 MS.gene94237:CDS 30.0%
GGGATATATAATGTAAGAGA+TGG - chr5.4:65494966-65494985 MS.gene94237:CDS 30.0%
TACAAACATCTTTCTGTTCT+TGG + chr5.4:65494174-65494193 None:intergenic 30.0%
TATGTTGCGAGTAAATATCA+CGG - chr5.4:65494573-65494592 MS.gene94237:CDS 30.0%
! CGTTTCGATTTTATAAAAGC+GGG - chr5.4:65494471-65494490 MS.gene94237:CDS 30.0%
! CTTTTAATCTTGGTCATAAG+AGG - chr5.4:65494604-65494623 MS.gene94237:CDS 30.0%
! TATACAAGTTTATCTGAGGT+CGG - chr5.4:65494366-65494385 MS.gene94237:CDS 30.0%
!! GGAGACAATGTTTTATTCTT+GGG - chr5.4:65494816-65494835 MS.gene94237:CDS 30.0%
!! TGGAGACAATGTTTTATTCT+TGG - chr5.4:65494815-65494834 MS.gene94237:CDS 30.0%
!!! TGAATCTTTTTTCAGACAAC+CGG + chr5.4:65494880-65494899 None:intergenic 30.0%
AACTTGTATATGATCGATCG+AGG + chr5.4:65494356-65494375 None:intergenic 35.0%
ACTTGTATATGATCGATCGA+GGG + chr5.4:65494355-65494374 None:intergenic 35.0%
ACTTGTGGTCCATTTCATAT+AGG - chr5.4:65494945-65494964 MS.gene94237:CDS 35.0%
AGAAGGATGCAATAGTGTTA+TGG + chr5.4:65494304-65494323 None:intergenic 35.0%
AGCTTAACCATCAAAATCAG+CGG - chr5.4:65494115-65494134 MS.gene94237:CDS 35.0%
ATAGGAGCAACATTCTTAGA+AGG + chr5.4:65494321-65494340 None:intergenic 35.0%
ATGGATGTTATGTTCTATGG+AGG - chr5.4:65494543-65494562 MS.gene94237:CDS 35.0%
CGAATTCAAACTTGTACACT+TGG + chr5.4:65494749-65494768 None:intergenic 35.0%
CTTGTGGTCCATTTCATATA+GGG - chr5.4:65494946-65494965 MS.gene94237:CDS 35.0%
GATAACTCCAAATCCATTTC+AGG - chr5.4:65494626-65494645 MS.gene94237:CDS 35.0%
GGCTAAATCCACAACAAATA+TGG + chr5.4:65494248-65494267 None:intergenic 35.0%
GTGAAGATTATGATGATCAG+AGG + chr5.4:65494994-65495013 None:intergenic 35.0%
TGAAAAGCTATGTCCTGAAA+TGG + chr5.4:65494642-65494661 None:intergenic 35.0%
! ATACAAGTTTATCTGAGGTC+GGG - chr5.4:65494367-65494386 MS.gene94237:CDS 35.0%
! GCTTTTCATGGAAGGTTTAA+AGG - chr5.4:65494654-65494673 MS.gene94237:CDS 35.0%
! TTTCAGGACATAGCTTTTCA+TGG - chr5.4:65494642-65494661 MS.gene94237:CDS 35.0%
!! ATTTGTTGTGGATTTAGCCA+TGG - chr5.4:65494249-65494268 MS.gene94237:CDS 35.0%
!!! TTCTAAACCGCTGATTTTGA+TGG + chr5.4:65494125-65494144 None:intergenic 35.0%
AACCATGCTGGACTTATGTA+TGG - chr5.4:65494496-65494515 MS.gene94237:CDS 40.0%
AATCATTAGAGGGAGACTTG+TGG - chr5.4:65494688-65494707 MS.gene94237:CDS 40.0%
ATAAAGGAATGATGCCAGCT+CGG + chr5.4:65494525-65494544 None:intergenic 40.0%
ATCCATACATAAGTCCAGCA+TGG + chr5.4:65494501-65494520 None:intergenic 40.0%
ATGCTAAAGCTTGAGAGTCT+TGG - chr5.4:65494795-65494814 MS.gene94237:CDS 40.0%
GCAATTCACACTAGCATTCA+TGG - chr5.4:65494447-65494466 MS.gene94237:CDS 40.0%
GCCGCAACCATATAATCATT+TGG + chr5.4:65494429-65494448 None:intergenic 40.0%
GGAACTAGATTGGGCAAATT+TGG - chr5.4:65493996-65494015 MS.gene94237:CDS 40.0%
GTATCAGCTTCATACTTCTC+CGG - chr5.4:65494858-65494877 MS.gene94237:CDS 40.0%
GTTCTTGGGATCATTAGCAT+GGG + chr5.4:65494159-65494178 None:intergenic 40.0%
TAATCGCCAATTCTTGCACA+CGG + chr5.4:65494088-65494107 None:intergenic 40.0%
TGTTCTTGGGATCATTAGCA+TGG + chr5.4:65494160-65494179 None:intergenic 40.0%
TTTGAATTCGATGCTCAGAC+CGG - chr5.4:65494759-65494778 MS.gene94237:CDS 40.0%
TTTGGTAAAACCCAGAAAGG+TGG + chr5.4:65495044-65495063 None:intergenic 40.0%
! AGGACATAGCTTTTCATGGA+AGG - chr5.4:65494646-65494665 MS.gene94237:CDS 40.0%
! GCTATGTCCTGAAATGGATT+TGG + chr5.4:65494636-65494655 None:intergenic 40.0%
ATTAGAGGGAGACTTGTGGA+TGG - chr5.4:65494692-65494711 MS.gene94237:CDS 45.0%
CTATTGTTGCAAGCCAACCA+TGG + chr5.4:65494269-65494288 None:intergenic 45.0%
GAGCTGGCATCATTCCTTTA+TGG - chr5.4:65494524-65494543 MS.gene94237:CDS 45.0%
GTATATGATCGATCGAGGGT+TGG + chr5.4:65494351-65494370 None:intergenic 45.0%
GTTGTGGATTTAGCCATGGT+TGG - chr5.4:65494253-65494272 MS.gene94237:CDS 45.0%
TAAAGGAATGATGCCAGCTC+GGG + chr5.4:65494524-65494543 None:intergenic 45.0%
TCGAGGGTTGGAAGACTAAT+AGG + chr5.4:65494339-65494358 None:intergenic 45.0%
! TTTATCTGAGGTCGGGTACA+AGG - chr5.4:65494374-65494393 MS.gene94237:CDS 45.0%
CTTATGTATGGATCCCGAGC+TGG - chr5.4:65494508-65494527 MS.gene94237:CDS 50.0%
GGTAAAACCCAGAAAGGTGG+TGG + chr5.4:65495041-65495060 None:intergenic 50.0%
!! TTGGTGCCGTGTGCAAGAAT+TGG - chr5.4:65494079-65494098 MS.gene94237:CDS 50.0%
TAAAAGCGGGCGAACCATGC+TGG - chr5.4:65494484-65494503 MS.gene94237:CDS 55.0%
AGCAGCAGCCACCACCTTTC+TGG - chr5.4:65495030-65495049 MS.gene94237:CDS 60.0%
GCAGCAGCCACCACCTTTCT+GGG - chr5.4:65495031-65495050 MS.gene94237:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 65493994 65495073 65493994 ID=MS.gene94237
chr5.4 mRNA 65493994 65495073 65493994 ID=MS.gene94237.t1;Parent=MS.gene94237
chr5.4 exon 65493994 65495073 65493994 ID=MS.gene94237.t1.exon1;Parent=MS.gene94237.t1
chr5.4 CDS 65493994 65495073 65493994 ID=cds.MS.gene94237.t1;Parent=MS.gene94237.t1
Gene Sequence

>MS.gene94237

ATGGAACTAGATTGGGCAAATTTGGAAGCACTTGCACTTGATTTAATTCTTGATAAGTTGGAAGAACAAATAGATCACATTTGTTTTGGTGCCGTGTGCAAGAATTGGCGATTAATTGCAAAGCTTAACCATCAAAATCAGCGGTTTAGAACTCATGTACTACCCATGCTAATGATCCCAAGAACAGAAAGATGTTTGTATAGCATTTCAGCCAAAAAAATGTATCCATTTAAATTGCCAAATTCTTATTACCATATTTGTTGTGGATTTAGCCATGGTTGGCTTGCAACAATAGATAAAAGTCATGCCATAACACTATTGCATCCTTCTAAGAATGTTGCTCCTATTAGTCTTCCAACCCTCGATCGATCATATACAAGTTTATCTGAGGTCGGGTACAAGGTCACTCTTTCTGCTGACCCTATAACAAGACCAAATGATTATATGGTTGCGGCAATTCACACTAGCATTCATGGACGTTTCGATTTTATAAAAGCGGGCGAACCATGCTGGACTTATGTATGGATCCCGAGCTGGCATCATTCCTTTATGGATGTTATGTTCTATGGAGGTTTAGTTTATGTTGCGAGTAAATATCACGGTATAGTATCTTTTAATCTTGGTCATAAGAGGATAACTCCAAATCCATTTCAGGACATAGCTTTTCATGGAAGGTTTAAAGGTTATCTTGTGAAATCATTAGAGGGAGACTTGTGGATGGTGAAAAGATTTTTTACTTTTAAGAATGATTTCCAAGTGTACAAGTTTGAATTCGATGCTCAGACCGGAAAGTTTAAACAAATGCTAAAGCTTGAGAGTCTTGGAGACAATGTTTTATTCTTGGGAGACACTGATTCAATTTCTGTATCAGCTTCATACTTCTCCGGTTGTCTGAAAAAAGATTCAATTTATTACACAGAAAGTATTAATGCAAACAAGTTAGATTGTGTAACTTGTGGTCCATTTCATATAGGGATATATAATGTAAGAGATGGAACCTCTGATCATCATAATCTTCACAATCATTTCTTCAACAAGCAGCAGCCACCACCTTTCTGGGTTTTACCAAATTTCCAATGA

Protein sequence

>MS.gene94237.t1

MELDWANLEALALDLILDKLEEQIDHICFGAVCKNWRLIAKLNHQNQRFRTHVLPMLMIPRTERCLYSISAKKMYPFKLPNSYYHICCGFSHGWLATIDKSHAITLLHPSKNVAPISLPTLDRSYTSLSEVGYKVTLSADPITRPNDYMVAAIHTSIHGRFDFIKAGEPCWTYVWIPSWHHSFMDVMFYGGLVYVASKYHGIVSFNLGHKRITPNPFQDIAFHGRFKGYLVKSLEGDLWMVKRFFTFKNDFQVYKFEFDAQTGKFKQMLKLESLGDNVLFLGDTDSISVSASYFSGCLKKDSIYYTESINANKLDCVTCGPFHIGIYNVRDGTSDHHNLHNHFFNKQQPPPFWVLPNFQ