AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27637

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27637.t1 MTR_7g077620 63.102 374 126 9 1 367 1 369 1.78e-150 430
MS.gene27637.t1 MTR_7g077650 58.549 386 130 13 1 370 1 372 3.75e-137 395
MS.gene27637.t1 MTR_7g077660 59.467 375 133 11 5 370 4 368 7.23e-134 387
MS.gene27637.t1 MTR_7g076100 69.957 233 65 4 141 370 70 300 2.78e-103 306
MS.gene27637.t1 MTR_7g076100 79.630 54 11 0 1 54 1 54 2.82e-22 95.9
MS.gene27637.t1 MTR_7g076110 56.949 295 116 5 85 370 21 313 1.76e-102 305
MS.gene27637.t1 MTR_1g108740 42.480 379 169 14 3 368 2 344 4.52e-77 241
MS.gene27637.t1 MTR_5g038260 41.414 396 183 18 6 370 6 383 8.25e-74 234
MS.gene27637.t1 MTR_5g038230 39.949 393 163 17 6 368 4 353 2.32e-67 216
MS.gene27637.t1 MTR_5g082770 43.831 308 139 9 6 302 4 288 2.72e-64 206
MS.gene27637.t1 MTR_1g064570 36.277 419 193 14 5 370 3 400 5.54e-62 204
MS.gene27637.t1 MTR_7g076130 76.613 124 26 2 167 287 25 148 1.93e-60 192
MS.gene27637.t1 MTR_1g016150 47.317 205 99 5 3 201 2 203 2.04e-48 164
MS.gene27637.t1 MTR_1g016150 58.462 65 21 2 252 316 177 235 1.50e-11 64.3
MS.gene27637.t1 MTR_5g082780 40.948 232 95 10 112 325 1 208 3.15e-41 145
MS.gene27637.t1 MTR_1g108750 45.455 154 50 5 6 158 4 124 8.46e-26 100
MS.gene27637.t1 MTR_3g114970 23.181 371 204 16 5 337 3 330 5.78e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATCATTTGGACTTGTAAT+TGG 0.148563 7.2:+29682632 None:intergenic
TTCGCATAGTAAGTAAAATT+AGG 0.191950 7.2:+29682422 None:intergenic
AAAGAGCAAGCCAACCATTT+TGG 0.203122 7.2:-29682568 MS.gene27637:CDS
ACCATCAAAACAATCAATTT+AGG 0.232527 7.2:-29682943 MS.gene27637:CDS
GATATACTACTACACTTAAA+AGG 0.276632 7.2:+29682710 None:intergenic
AATGAACAAGCTTGAGAGTT+TGG 0.278023 7.2:-29682216 MS.gene27637:CDS
ATTTGGTTGGTTTGCATTAG+TGG 0.297529 7.2:-29682762 MS.gene27637:CDS
AAGATCACCAATGTTGTAAA+TGG 0.306746 7.2:+29682598 None:intergenic
TAGTTTGGTTCTTGACAAAT+TGG 0.317439 7.2:-29683032 MS.gene27637:CDS
TTTCCCGTATGAAAATTATA+AGG 0.318336 7.2:-29682799 MS.gene27637:CDS
CTTTGTGACTTATGCTATAC+AGG 0.330550 7.2:+29682840 None:intergenic
GTTGAGATGTGGCAGAATAA+TGG 0.333023 7.2:+29682685 None:intergenic
AGAAGAACTGCCTCTTAGTT+TGG 0.335492 7.2:-29683047 MS.gene27637:CDS
TCGCATAGTAAGTAAAATTA+GGG 0.335896 7.2:+29682423 None:intergenic
AAGATCACATAGAGCATGTT+AGG 0.337175 7.2:-29683009 MS.gene27637:CDS
TTGAGATGTGGCAGAATAAT+GGG 0.362137 7.2:+29682686 None:intergenic
GTTGAACTTGAAGGATATAA+TGG 0.375803 7.2:+29682466 None:intergenic
TGAGAAGATATTTCATCAAT+TGG 0.385439 7.2:-29682310 MS.gene27637:CDS
AAATTAGGGTTATTTGAGTT+AGG 0.386206 7.2:+29682437 None:intergenic
CGCATCCCCTTGAAGGAAAT+CGG 0.389019 7.2:+29682023 None:intergenic
AATCATTTGGACTTGTAATT+GGG 0.394312 7.2:+29682633 None:intergenic
AAAACCTCGCATCCCCTTGA+AGG 0.402319 7.2:+29682016 None:intergenic
GGGTTCGACCGCAATTTCAA+TGG 0.414532 7.2:-29681992 MS.gene27637:CDS
AAAAGGATTGACTAGAGTTA+TGG 0.415743 7.2:+29682727 None:intergenic
TATGAAAATTATAAGGTGTA+TGG 0.418200 7.2:-29682792 MS.gene27637:CDS
CACATAGAGCATGTTAGGTT+TGG 0.419463 7.2:-29683004 MS.gene27637:CDS
AGGTGTATGGACTCTTCATT+TGG 0.419747 7.2:-29682779 MS.gene27637:CDS
GTAGACATCAAGTTTATATG+AGG 0.436334 7.2:+29682277 None:intergenic
GTATGGACTCTTCATTTGGT+TGG 0.436531 7.2:-29682775 MS.gene27637:CDS
TGATGTCTACAAATTAGAAC+TGG 0.443705 7.2:-29682264 MS.gene27637:CDS
TGTGCTATATGAATCCAAAA+TGG 0.443882 7.2:+29682554 None:intergenic
GCTGCAATGACATAATCATT+TGG 0.444853 7.2:+29682620 None:intergenic
ACCAATACCATTCGTCTTCG+AGG 0.449702 7.2:+29682894 None:intergenic
TCCTCGAAGACGAATGGTAT+TGG 0.465751 7.2:-29682895 MS.gene27637:CDS
GTCTTCGAGGATCATGATCA+TGG 0.468105 7.2:+29682907 None:intergenic
CAATGAGGAAGGTGGCAGAC+TGG 0.474514 7.2:-29683078 MS.gene27637:CDS
GGTTCGACCGCAATTTCAAT+GGG 0.479149 7.2:-29681991 MS.gene27637:CDS
AGGAGGATTGTTGAACTTGA+AGG 0.483112 7.2:+29682457 None:intergenic
TTGGTGTCGTGTGCAAGAAT+TGG 0.497007 7.2:-29682985 MS.gene27637:CDS
AATCATTGAATGGAGACATA+TGG 0.507395 7.2:-29682337 MS.gene27637:CDS
TTGTCAAGAACCAAACTAAG+AGG 0.512788 7.2:+29683037 None:intergenic
ATTTATGACCATGGTATGCA+TGG 0.513250 7.2:-29682101 MS.gene27637:CDS
AATGAGGAAGGTGGCAGACT+GGG 0.522188 7.2:-29683077 MS.gene27637:CDS
ATATAATGGTGTGTCCTTCA+CGG 0.524661 7.2:+29682480 None:intergenic
ATTGCAGCCATTTACAACAT+TGG 0.532590 7.2:-29682605 MS.gene27637:CDS
GCAGACAAGGTAACCTTGTA+GGG 0.532760 7.2:+29682659 None:intergenic
TTAGGGTTATTTGAGTTAGG+AGG 0.546912 7.2:+29682440 None:intergenic
AGCAGACAAGGTAACCTTGT+AGG 0.548503 7.2:+29682658 None:intergenic
ACATCTCAACTCTCCCTACA+AGG 0.555243 7.2:-29682672 MS.gene27637:CDS
CTCAGGCTTGGCAATGAGGA+AGG 0.555628 7.2:-29683089 None:intergenic
GAGATCTATAATGCGAAGGA+TGG 0.563178 7.2:-29682068 MS.gene27637:CDS
TTCTAACTTGGAGAAACGAG+AGG 0.573200 7.2:+29682142 None:intergenic
TTGCTATTTGCCGACCGTGA+AGG 0.573377 7.2:-29682494 MS.gene27637:CDS
TGTAATTGGGTCAGCAGACA+AGG 0.582291 7.2:+29682646 None:intergenic
TCTTCGAGGATCATGATCAT+GGG 0.595038 7.2:+29682908 None:intergenic
TATCAATCCCATTGAAATTG+CGG 0.600714 7.2:+29681984 None:intergenic
ATTTCCTTCAAGGGGATGCG+AGG 0.607717 7.2:-29682020 MS.gene27637:CDS
CTATATGAATCCAAAATGGT+TGG 0.609689 7.2:+29682558 None:intergenic
TCGAGGATCATGATCATGGG+TGG 0.616682 7.2:+29682911 None:intergenic
GTTAGAGATCTATAATGCGA+AGG 0.619212 7.2:-29682072 MS.gene27637:CDS
AATGGTGTGTCCTTCACGGT+CGG 0.620738 7.2:+29682484 None:intergenic
TCATGATCCTCGAAGACGAA+TGG 0.621729 7.2:-29682901 MS.gene27637:CDS
GTGCAAGAATTGGCAATCAG+TGG 0.629037 7.2:-29682975 MS.gene27637:CDS
TTTGTGACTTATGCTATACA+GGG 0.646384 7.2:+29682841 None:intergenic
ACTCTAAACCATGCATACCA+TGG 0.661226 7.2:+29682093 None:intergenic
AGGCTTGGCAATGAGGAAGG+TGG 0.663381 7.2:-29683086 None:intergenic
TTGTAGGGAGAGTTGAGATG+TGG 0.664401 7.2:+29682674 None:intergenic
ATGCGAAGATAGCTAGCACA+AGG 0.689632 7.2:-29682406 MS.gene27637:CDS
ATTGAATGGAGACATATGGA+TGG 0.693585 7.2:-29682333 MS.gene27637:CDS
TTAGAACTGGATGCCCAGAG+TGG 0.702949 7.2:-29682251 MS.gene27637:CDS
ACACAGAAACTGAATCACCA+TGG 0.719525 7.2:+29682168 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATGTTGTATTTTATAA+AGG - chr7.2:29682544-29682563 MS.gene27637:CDS 10.0%
!!! ATTTATTTTATTTATGACCA+TGG - chr7.2:29682952-29682971 MS.gene27637:CDS 15.0%
!! TATGAAAATTATAAGGTGTA+TGG - chr7.2:29682270-29682289 MS.gene27637:CDS 20.0%
!!! AAATCTTTTCATAATAGCAT+TGG + chr7.2:29682696-29682715 None:intergenic 20.0%
!!! AATCTTTTCATAATAGCATT+GGG + chr7.2:29682695-29682714 None:intergenic 20.0%
!!! TACACCTTATAATTTTCATA+CGG + chr7.2:29682270-29682289 None:intergenic 20.0%
!!! TATTTTGTGAAATCATTGAA+TGG - chr7.2:29682715-29682734 MS.gene27637:CDS 20.0%
! AATCATTTGGACTTGTAATT+GGG + chr7.2:29682432-29682451 None:intergenic 25.0%
! ACCATCAAAACAATCAATTT+AGG - chr7.2:29682119-29682138 MS.gene27637:CDS 25.0%
! GATATACTACTACACTTAAA+AGG + chr7.2:29682355-29682374 None:intergenic 25.0%
! TAATCATTTGGACTTGTAAT+TGG + chr7.2:29682433-29682452 None:intergenic 25.0%
! TCGCATAGTAAGTAAAATTA+GGG + chr7.2:29682642-29682661 None:intergenic 25.0%
! TGAGAAGATATTTCATCAAT+TGG - chr7.2:29682752-29682771 MS.gene27637:CDS 25.0%
! TTCGCATAGTAAGTAAAATT+AGG + chr7.2:29682643-29682662 None:intergenic 25.0%
! TTTCCCGTATGAAAATTATA+AGG - chr7.2:29682263-29682282 MS.gene27637:CDS 25.0%
!! AAATTAGGGTTATTTGAGTT+AGG + chr7.2:29682628-29682647 None:intergenic 25.0%
!! AATCGAATCTTTTTCTAACT+TGG + chr7.2:29682935-29682954 None:intergenic 25.0%
!! ACACCTTATAATTTTCATAC+GGG + chr7.2:29682269-29682288 None:intergenic 25.0%
!!! GAAGACAACATTTTATTTGT+CGG - chr7.2:29682868-29682887 MS.gene27637:CDS 25.0%
!!! TCCTAAATTGATTGTTTTGA+TGG + chr7.2:29682123-29682142 None:intergenic 25.0%
AAAAGGATTGACTAGAGTTA+TGG + chr7.2:29682338-29682357 None:intergenic 30.0%
AAGATCACCAATGTTGTAAA+TGG + chr7.2:29682467-29682486 None:intergenic 30.0%
AATCATTGAATGGAGACATA+TGG - chr7.2:29682725-29682744 MS.gene27637:CDS 30.0%
ATACGGGAAAAAAATGTAAC+AGG + chr7.2:29682253-29682272 None:intergenic 30.0%
CTATATGAATCCAAAATGGT+TGG + chr7.2:29682507-29682526 None:intergenic 30.0%
GTAGACATCAAGTTTATATG+AGG + chr7.2:29682788-29682807 None:intergenic 30.0%
GTTGAACTTGAAGGATATAA+TGG + chr7.2:29682599-29682618 None:intergenic 30.0%
TACGGGAAAAAAATGTAACA+GGG + chr7.2:29682252-29682271 None:intergenic 30.0%
TGATGTCTACAAATTAGAAC+TGG - chr7.2:29682798-29682817 MS.gene27637:CDS 30.0%
TGTGCTATATGAATCCAAAA+TGG + chr7.2:29682511-29682530 None:intergenic 30.0%
TTTGTGACTTATGCTATACA+GGG + chr7.2:29682224-29682243 None:intergenic 30.0%
!! ATTTTATTTGTCGGAAACCA+TGG - chr7.2:29682877-29682896 MS.gene27637:CDS 30.0%
!! TAGTTTGGTTCTTGACAAAT+TGG - chr7.2:29682030-29682049 MS.gene27637:CDS 30.0%
!! TTTTCATAATAGCATTGGGA+TGG + chr7.2:29682691-29682710 None:intergenic 30.0%
!!! ATTTTTACCGATTTCCTTCA+AGG - chr7.2:29683032-29683051 MS.gene27637:CDS 30.0%
!!! TTTTTACCGATTTCCTTCAA+GGG - chr7.2:29683033-29683052 MS.gene27637:CDS 30.0%
AAGATCACATAGAGCATGTT+AGG - chr7.2:29682053-29682072 MS.gene27637:CDS 35.0%
AATGAACAAGCTTGAGAGTT+TGG - chr7.2:29682846-29682865 MS.gene27637:CDS 35.0%
ATATAATGGTGTGTCCTTCA+CGG + chr7.2:29682585-29682604 None:intergenic 35.0%
ATTGAATGGAGACATATGGA+TGG - chr7.2:29682729-29682748 MS.gene27637:CDS 35.0%
ATTGCAGCCATTTACAACAT+TGG - chr7.2:29682457-29682476 MS.gene27637:CDS 35.0%
ATTTATGACCATGGTATGCA+TGG - chr7.2:29682961-29682980 MS.gene27637:CDS 35.0%
CTTTGTGACTTATGCTATAC+AGG + chr7.2:29682225-29682244 None:intergenic 35.0%
GCTGCAATGACATAATCATT+TGG + chr7.2:29682445-29682464 None:intergenic 35.0%
GTTAGAGATCTATAATGCGA+AGG - chr7.2:29682990-29683009 MS.gene27637:CDS 35.0%
TTGAGATGTGGCAGAATAAT+GGG + chr7.2:29682379-29682398 None:intergenic 35.0%
TTGTCAAGAACCAAACTAAG+AGG + chr7.2:29682028-29682047 None:intergenic 35.0%
! TTAGGGTTATTTGAGTTAGG+AGG + chr7.2:29682625-29682644 None:intergenic 35.0%
!! ATTTGGTTGGTTTGCATTAG+TGG - chr7.2:29682300-29682319 MS.gene27637:CDS 35.0%
!! TTGTTCAAGTTTTCCACTCT+GGG + chr7.2:29682827-29682846 None:intergenic 35.0%
!! TTTGTTCAAGTTTTCCACTC+TGG + chr7.2:29682828-29682847 None:intergenic 35.0%
!!! TTTTACCGATTTCCTTCAAG+GGG - chr7.2:29683034-29683053 MS.gene27637:CDS 35.0%
ACACAGAAACTGAATCACCA+TGG + chr7.2:29682897-29682916 None:intergenic 40.0%
ACTCTAAACCATGCATACCA+TGG + chr7.2:29682972-29682991 None:intergenic 40.0%
AGAAGAACTGCCTCTTAGTT+TGG - chr7.2:29682015-29682034 MS.gene27637:CDS 40.0%
AGGAGGATTGTTGAACTTGA+AGG + chr7.2:29682608-29682627 None:intergenic 40.0%
AGGTGTATGGACTCTTCATT+TGG - chr7.2:29682283-29682302 MS.gene27637:CDS 40.0%
CACATAGAGCATGTTAGGTT+TGG - chr7.2:29682058-29682077 MS.gene27637:CDS 40.0%
GAGATCTATAATGCGAAGGA+TGG - chr7.2:29682994-29683013 MS.gene27637:CDS 40.0%
GTATGGACTCTTCATTTGGT+TGG - chr7.2:29682287-29682306 MS.gene27637:CDS 40.0%
GTTGAGATGTGGCAGAATAA+TGG + chr7.2:29682380-29682399 None:intergenic 40.0%
TCTTCGAGGATCATGATCAT+GGG + chr7.2:29682157-29682176 None:intergenic 40.0%
TTCTAACTTGGAGAAACGAG+AGG + chr7.2:29682923-29682942 None:intergenic 40.0%
! AAAGAGCAAGCCAACCATTT+TGG - chr7.2:29682494-29682513 MS.gene27637:CDS 40.0%
ACATCTCAACTCTCCCTACA+AGG - chr7.2:29682390-29682409 MS.gene27637:CDS 45.0%
ACCAATACCATTCGTCTTCG+AGG + chr7.2:29682171-29682190 None:intergenic 45.0%
AGCAGACAAGGTAACCTTGT+AGG + chr7.2:29682407-29682426 None:intergenic 45.0%
ATGCGAAGATAGCTAGCACA+AGG - chr7.2:29682656-29682675 MS.gene27637:CDS 45.0%
GCAGACAAGGTAACCTTGTA+GGG + chr7.2:29682406-29682425 None:intergenic 45.0%
GGTTCGACCGCAATTTCAAT+GGG - chr7.2:29683071-29683090 MS.gene27637:CDS 45.0%
GTCTTCGAGGATCATGATCA+TGG + chr7.2:29682158-29682177 None:intergenic 45.0%
GTGCAAGAATTGGCAATCAG+TGG - chr7.2:29682087-29682106 MS.gene27637:CDS 45.0%
TCATGATCCTCGAAGACGAA+TGG - chr7.2:29682161-29682180 MS.gene27637:CDS 45.0%
TCCTCGAAGACGAATGGTAT+TGG - chr7.2:29682167-29682186 MS.gene27637:CDS 45.0%
TGTAATTGGGTCAGCAGACA+AGG + chr7.2:29682419-29682438 None:intergenic 45.0%
TTGTAGGGAGAGTTGAGATG+TGG + chr7.2:29682391-29682410 None:intergenic 45.0%
!! TTGGTGTCGTGTGCAAGAAT+TGG - chr7.2:29682077-29682096 MS.gene27637:CDS 45.0%
AAAACCTCGCATCCCCTTGA+AGG + chr7.2:29683049-29683068 None:intergenic 50.0%
AATGGTGTGTCCTTCACGGT+CGG + chr7.2:29682581-29682600 None:intergenic 50.0%
ATTTCCTTCAAGGGGATGCG+AGG - chr7.2:29683042-29683061 MS.gene27637:CDS 50.0%
CGCATCCCCTTGAAGGAAAT+CGG + chr7.2:29683042-29683061 None:intergenic 50.0%
GGGTTCGACCGCAATTTCAA+TGG - chr7.2:29683070-29683089 MS.gene27637:CDS 50.0%
TCGAGGATCATGATCATGGG+TGG + chr7.2:29682154-29682173 None:intergenic 50.0%
TTAGAACTGGATGCCCAGAG+TGG - chr7.2:29682811-29682830 MS.gene27637:CDS 50.0%
! CAAGGGGATGCGAGGTTTTT+GGG - chr7.2:29683050-29683069 MS.gene27637:CDS 50.0%
! TCAAGGGGATGCGAGGTTTT+TGG - chr7.2:29683049-29683068 MS.gene27637:CDS 50.0%
!! TTGCTATTTGCCGACCGTGA+AGG - chr7.2:29682568-29682587 MS.gene27637:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 29681986 29683098 29681986 ID=MS.gene27637
chr7.2 mRNA 29681986 29683098 29681986 ID=MS.gene27637.t1;Parent=MS.gene27637
chr7.2 exon 29681986 29683098 29681986 ID=MS.gene27637.t1.exon1;Parent=MS.gene27637.t1
chr7.2 CDS 29681986 29683098 29681986 ID=cds.MS.gene27637.t1;Parent=MS.gene27637.t1
Gene Sequence

>MS.gene27637

ATGAGGAAGGTGGCAGACTGGGCTAACTTAGAAGAACTGCCTCTTAGTTTGGTTCTTGACAAATTGGAAGATCACATAGAGCATGTTAGGTTTGGTGTCGTGTGCAAGAATTGGCAATCAGTGGCAAAGCTTAACCATCAAAACAATCAATTTAGGATTAATAAACCACCCATGATCATGATCCTCGAAGACGAATGGTATTGGTCTTATACGCATACAAAAAACGAGAAGAGATCCCTGTATAGCATAAGTCACAAAGAGTACCCTGTTACATTTTTTTCCCGTATGAAAATTATAAGGTGTATGGACTCTTCATTTGGTTGGTTTGCATTAGTGGACAATAACAATGCCATAACTCTAGTCAATCCTTTTAAGTGTAGTAGTATATCTCCCATTATTCTGCCACATCTCAACTCTCCCTACAAGGTTACCTTGTCTGCTGACCCAATTACAAGTCCAAATGATTATGTCATTGCAGCCATTTACAACATTGGTGATCTTGCTTTCAAAAGAGCAAGCCAACCATTTTGGATTCATATAGCACATGAATTTGCTATCAATAATGTTGTATTTTATAAAGGCTTGCTATTTGCCGACCGTGAAGGACACACCATTATATCCTTCAAGTTCAACAATCCTCCTAACTCAAATAACCCTAATTTTACTTACTATGCGAAGATAGCTAGCACAAGGTATAGTGTTCCATCCCAATGCTATTATGAAAAGATTTATTTTGTGAAATCATTGAATGGAGACATATGGATGGTGAGAAGATATTTCATCAATTGGTATGACAAGTCCTCATATAAACTTGATGTCTACAAATTAGAACTGGATGCCCAGAGTGGAAAACTTGAACAAATGAACAAGCTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAAACCATGGTGATTCAGTTTCTGTGTCAGCCTCTCGTTTCTCCAAGTTAGAAAAAGATTCGATTTATTTTATTTATGACCATGGTATGCATGGTTTAGAGTTAGAGATCTATAATGCGAAGGATGGAAGCTGTCAGAGACAATTTTTACCGATTTCCTTCAAGGGGATGCGAGGTTTTTGGGTTCGACCGCAATTTCAATGGGATTGA
Protein sequence

>MS.gene27637.t1

MRKVADWANLEELPLSLVLDKLEDHIEHVRFGVVCKNWQSVAKLNHQNNQFRINKPPMIMILEDEWYWSYTHTKNEKRSLYSISHKEYPVTFFSRMKIIRCMDSSFGWFALVDNNNAITLVNPFKCSSISPIILPHLNSPYKVTLSADPITSPNDYVIAAIYNIGDLAFKRASQPFWIHIAHEFAINNVVFYKGLLFADREGHTIISFKFNNPPNSNNPNFTYYAKIASTRYSVPSQCYYEKIYFVKSLNGDIWMVRRYFINWYDKSSYKLDVYKLELDAQSGKLEQMNKLESLEDNILFVGNHGDSVSVSASRFSKLEKDSIYFIYDHGMHGLELEIYNAKDGSCQRQFLPISFKGMRGFWVRPQFQWD