AlfalfaGEDB | MS.gene86085

M. sativa cultivar XinJiangDaYe / MS.gene86085

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86085.t1	XP_013468204.1	75.6	254	57	3	12	263	150	400	3.50E-110	407.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86085.t1	Q9FLP7	23.4	231	147	8	30	258	153	355	1.8e-06	54.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86085.t1	A0A072VKT8	75.6	254	57	3	12	263	150	400	2.6e-110	407.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053074	MS.gene86085	0.800912	1.15E-48	-1.69E-46
MS.gene057060	MS.gene86085	0.803253	3.81E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene86085	MS.gene85835	PPI
MS.gene86085	MS.gene032785	PPI
MS.gene86085	MS.gene97317	PPI
MS.gene42222	MS.gene86085	PPI
MS.gene99022	MS.gene86085	PPI
MS.gene86085	MS.gene42224	PPI
MS.gene023893	MS.gene86085	PPI
MS.gene86085	MS.gene27754	PPI
MS.gene86085	MS.gene73726	PPI
MS.gene27750	MS.gene86085	PPI
MS.gene86085	MS.gene035135	PPI
MS.gene52697	MS.gene86085	PPI
MS.gene86085	MS.gene27748	PPI
MS.gene86085	MS.gene27637	PPI
MS.gene86085	MS.gene023784	PPI
MS.gene42430	MS.gene86085	PPI
MS.gene86085	MS.gene52254	PPI
MS.gene86085	MS.gene023890	PPI
MS.gene86085	MS.gene023888	PPI
MS.gene066289	MS.gene86085	PPI
MS.gene86085	MS.gene99023	PPI
MS.gene99024	MS.gene86085	PPI
MS.gene52163	MS.gene86085	PPI
MS.gene86085	MS.gene52259	PPI
MS.gene86085	MS.gene035137	PPI
MS.gene86085	MS.gene44542	PPI
MS.gene86085	MS.gene27747	PPI
MS.gene86085	MS.gene73460	PPI
MS.gene86085	MS.gene43191	PPI
MS.gene035137	MS.gene86085	PPI
MS.gene86085	MS.gene49592	PPI
MS.gene52253	MS.gene86085	PPI
MS.gene86085	MS.gene94239	PPI
MS.gene86085	MS.gene023892	PPI
MS.gene86085	MS.gene73723	PPI
MS.gene86085	MS.gene023785	PPI
MS.gene86085	MS.gene032784	PPI
MS.gene86085	MS.gene76061	PPI
MS.gene86085	MS.gene95450	PPI
MS.gene027042	MS.gene86085	PPI
MS.gene86085	MS.gene52262	PPI
MS.gene86085	MS.gene49594	PPI
MS.gene27753	MS.gene86085	PPI
MS.gene86085	MS.gene29654	PPI
MS.gene76061	MS.gene86085	PPI
MS.gene86085	MS.gene52253	PPI
MS.gene023891	MS.gene86085	PPI
MS.gene86085	MS.gene52255	PPI
MS.gene86085	MS.gene42431	PPI
MS.gene86085	MS.gene27639	PPI
MS.gene86085	MS.gene22627	PPI
MS.gene86085	MS.gene031136	PPI
MS.gene032784	MS.gene86085	PPI
MS.gene86085	MS.gene46208	PPI
MS.gene29654	MS.gene86085	PPI
MS.gene023785	MS.gene86085	PPI
MS.gene032780	MS.gene86085	PPI
MS.gene041287	MS.gene86085	PPI
MS.gene27745	MS.gene86085	PPI
MS.gene86085	MS.gene023787	PPI
MS.gene86085	MS.gene027042	PPI
MS.gene86085	MS.gene42433	PPI
MS.gene86085	MS.gene023893	PPI
MS.gene29651	MS.gene86085	PPI
MS.gene86085	MS.gene42432	PPI
MS.gene86085	MS.gene49593	PPI
MS.gene86085	MS.gene27635	PPI
MS.gene86085	MS.gene42429	PPI
MS.gene86085	MS.gene52913	PPI
MS.gene023787	MS.gene86085	PPI
MS.gene86085	MS.gene52261	PPI
MS.gene86085	MS.gene031137	PPI
MS.gene86085	MS.gene99022	PPI
MS.gene86085	MS.gene27751	PPI
MS.gene86085	MS.gene42223	PPI
MS.gene86085	MS.gene42428	PPI
MS.gene86085	MS.gene42226	PPI
MS.gene86085	MS.gene94237	PPI
MS.gene86085	MS.gene52263	PPI
MS.gene86085	MS.gene76056	PPI
MS.gene86085	MS.gene52166	PPI
MS.gene86085	MS.gene52164	PPI
MS.gene86085	MS.gene85826	PPI
MS.gene066291	MS.gene86085	PPI
MS.gene066292	MS.gene86085	PPI
MS.gene86085	MS.gene023887	PPI
MS.gene023889	MS.gene86085	PPI
MS.gene029514	MS.gene86085	PPI
MS.gene022086	MS.gene86085	PPI
MS.gene86085	MS.gene76059	PPI
MS.gene27749	MS.gene86085	PPI
MS.gene52258	MS.gene86085	PPI
MS.gene86085	MS.gene27636	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86085.t1	MTR_1g064570	75.591	254	57	3	12	263	150	400	1.16e-137	393
MS.gene86085.t1	MTR_5g038260	54.753	263	107	4	3	262	129	382	1.13e-94	283
MS.gene86085.t1	MTR_5g038230	48.846	260	99	5	3	261	127	353	8.29e-76	234
MS.gene86085.t1	MTR_6g023955	93.333	90	6	0	4	93	63	152	1.95e-54	175
MS.gene86085.t1	MTR_6g023955	82.258	62	11	0	202	263	149	210	4.02e-32	117
MS.gene86085.t1	MTR_5g082780	44.037	218	96	4	16	230	36	230	5.69e-51	167
MS.gene86085.t1	MTR_1g108740	42.000	250	106	7	14	261	132	344	2.29e-45	155
MS.gene86085.t1	MTR_7g076100	43.359	256	114	10	15	263	69	300	5.54e-44	150
MS.gene86085.t1	MTR_7g077660	40.449	267	104	10	14	263	140	368	3.27e-41	145
MS.gene86085.t1	MTR_7g077620	39.147	258	120	11	14	261	140	370	2.34e-39	141
MS.gene86085.t1	MTR_7g077650	38.095	273	107	12	14	263	139	372	4.20e-37	134
MS.gene86085.t1	MTR_7g076110	37.931	261	123	11	15	263	80	313	3.51e-36	130
MS.gene86085.t1	MTR_5g082770	47.977	173	75	4	15	187	133	290	1.57e-35	129
MS.gene86085.t1	MTR_7g076130	36.552	145	73	4	33	170	16	148	1.97e-16	74.7
MS.gene86085.t1	MTR_3g114990	28.444	225	139	9	15	235	174	380	9.58e-14	70.9
MS.gene86085.t1	MTR_1g016150	46.154	78	26	2	122	199	175	236	1.30e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 49 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTATGTCGGCTTCGTATTT+TGG	0.208690	6.3:+8608797	MS.gene86085:CDS
AATGTTAAAGCTTGATAGTT+TGG	0.243559	6.3:+8608736	MS.gene86085:CDS
TAAAAGCTGGACAAAAGTTT+TGG	0.255927	6.3:+8608363	MS.gene86085:CDS
TATGTTGTAGTTGCAATTTA+TGG	0.256703	6.3:+8608317	MS.gene86085:CDS
TGGCTTGTAAGGAAATTTAT+TGG	0.269879	6.3:+8608602	MS.gene86085:CDS
TTCATATCAAATGGTCCATT+AGG	0.289459	6.3:-8608887	None:intergenic
CTAACTCTTCTATATATATC+TGG	0.344601	6.3:-8608230	None:intergenic
TCATATCAAATGGTCCATTA+GGG	0.347171	6.3:-8608886	None:intergenic
CGAAGTGTATAAGTTGGAAT+TGG	0.356208	6.3:+8608688	MS.gene86085:CDS
ATGTTAAAGCTTGATAGTTT+GGG	0.382709	6.3:+8608737	MS.gene86085:CDS
TCCTAATGTTGTTTCACCAT+GGG	0.397638	6.3:+8608526	MS.gene86085:CDS
AGGGAGACTTGTGGCTTGTA+AGG	0.407147	6.3:+8608591	MS.gene86085:CDS
AGCTATTATTCCCACTGAAA+TGG	0.414384	6.3:-8609001	None:intergenic
TTATGAATGTAACGTTCATA+AGG	0.448018	6.3:+8608256	MS.gene86085:CDS
TGTCTTGCTTTCATAAAAGC+TGG	0.450654	6.3:+8608350	MS.gene86085:CDS
AAAATCTATAATGTAAAAGA+TGG	0.451916	6.3:+8608908	MS.gene86085:CDS
TATCGGCAAATCCTACAATC+AGG	0.460348	6.3:+8608285	MS.gene86085:CDS
GTTCACACGGATGCCACCTT+CGG	0.463341	6.3:+8608964	MS.gene86085:CDS
AAGATTCGAAGTGTATAAGT+TGG	0.476468	6.3:+8608682	MS.gene86085:CDS
GCACCGGATCCTTACCCTAA+TGG	0.478062	6.3:+8608872	MS.gene86085:CDS
TAATCGTGAGGCCTGATTGT+AGG	0.479756	6.3:-8608296	None:intergenic
CCCCATGGTGAAACAACATT+AGG	0.481080	6.3:-8608527	None:intergenic
TTCATCATCAATGTCATCAT+CGG	0.495737	6.3:-8608630	None:intergenic
AGCATTGCAATTTCGAACAT+TGG	0.498979	6.3:+8608942	MS.gene86085:CDS
TCATCATCAATGTCATCATC+GGG	0.501772	6.3:-8608629	None:intergenic
TAATGTTGTTTCACCATGGG+GGG	0.533333	6.3:+8608529	MS.gene86085:CDS
CACGGATGCCACCTTCGGTG+TGG	0.534966	6.3:+8608969	MS.gene86085:CDS
TAGTCTATGCTGCGGGACGA+TGG	0.540068	6.3:+8608441	MS.gene86085:CDS
TCAAATGGTCCATTAGGGTA+AGG	0.543154	6.3:-8608881	None:intergenic
TTCCTAATGTTGTTTCACCA+TGG	0.544368	6.3:+8608525	MS.gene86085:CDS
GGGTTCTTCCGCCATTTCAG+TGG	0.545118	6.3:+8608990	MS.gene86085:CDS
GGTTCTTCCGCCATTTCAGT+GGG	0.545364	6.3:+8608991	MS.gene86085:CDS
ACGGATGCCACCTTCGGTGT+GGG	0.551054	6.3:+8608970	MS.gene86085:CDS
TGATTATTTCGAAGAAGCAC+CGG	0.570964	6.3:+8608856	MS.gene86085:CDS
GGAAGAACCCACACCGAAGG+TGG	0.579383	6.3:-8608977	None:intergenic
GGTCCATTAGGGTAAGGATC+CGG	0.584997	6.3:-8608875	None:intergenic
AAAGGGTTAGTCTATGCTGC+GGG	0.586027	6.3:+8608434	MS.gene86085:CDS
TAGTGATTCTGTATCTATGT+CGG	0.590343	6.3:+8608784	MS.gene86085:CDS
CCTAATGTTGTTTCACCATG+GGG	0.597547	6.3:+8608527	MS.gene86085:CDS
ATTTCGAACATTGGTTCACA+CGG	0.602276	6.3:+8608951	MS.gene86085:CDS
GATAGTAACTTGCCTAGTAG+TGG	0.603948	6.3:+8608653	MS.gene86085:CDS
GGCGGAAGAACCCACACCGA+AGG	0.621085	6.3:-8608980	None:intergenic
TGCTTCTTCGAAATAATCAT+CGG	0.622108	6.3:-8608852	None:intergenic
TAAAGGGTTAGTCTATGCTG+CGG	0.625231	6.3:+8608433	MS.gene86085:CDS
TATTATTCCCACTGAAATGG+CGG	0.639125	6.3:-8608998	None:intergenic
AATCATTGGAGGGAGACTTG+TGG	0.640759	6.3:+8608582	MS.gene86085:CDS
TTGGAATTGGATCTTCAAAG+TGG	0.643699	6.3:+8608701	MS.gene86085:CDS
CTAATGTTGTTTCACCATGG+GGG	0.657132	6.3:+8608528	MS.gene86085:CDS
GCAACTACAACATAATCGTG+AGG	0.708433	6.3:-8608308	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATCTATAATGTAAAAGA+TGG	+	chr6.3:8608908-8608927	MS.gene86085:CDS	15.0%
!!!	TTATAGATTTTCATATCAAA+TGG	-	chr6.3:8608899-8608918	None:intergenic	15.0%
!!!	AGCGATGTTATATTTTATAA+AGG	+	chr6.3:8608416-8608435	MS.gene86085:CDS	20.0%
!!!	GCGATGTTATATTTTATAAA+GGG	+	chr6.3:8608417-8608436	MS.gene86085:CDS	20.0%
!!!	TTTTGGTAATTATCTACAAA+AGG	+	chr6.3:8608814-8608833	MS.gene86085:CDS	20.0%
!	CTAACTCTTCTATATATATC+TGG	-	chr6.3:8608233-8608252	None:intergenic	25.0%
!	TATGTTGTAGTTGCAATTTA+TGG	+	chr6.3:8608317-8608336	MS.gene86085:CDS	25.0%
!	TTATGAATGTAACGTTCATA+AGG	+	chr6.3:8608256-8608275	MS.gene86085:CDS	25.0%
!!	AATGTTAAAGCTTGATAGTT+TGG	+	chr6.3:8608736-8608755	MS.gene86085:CDS	25.0%
!!	AGCATATTTTGTGAAATCAT+TGG	+	chr6.3:8608568-8608587	MS.gene86085:CDS	25.0%
!!	ATATTTTGTGAAATCATTGG+AGG	+	chr6.3:8608571-8608590	MS.gene86085:CDS	25.0%
!!	ATGTTAAAGCTTGATAGTTT+GGG	+	chr6.3:8608737-8608756	MS.gene86085:CDS	25.0%
!!	TATTTTGTGAAATCATTGGA+GGG	+	chr6.3:8608572-8608591	MS.gene86085:CDS	25.0%
!!	TTTTGATATTTGTAACTCGA+AGG	+	chr6.3:8608478-8608497	MS.gene86085:CDS	25.0%
!!!	CGTTCATAAGGTTATTTTAT+CGG	+	chr6.3:8608268-8608287	MS.gene86085:CDS	25.0%
	AAGATTCGAAGTGTATAAGT+TGG	+	chr6.3:8608682-8608701	MS.gene86085:CDS	30.0%
	TAGTGATTCTGTATCTATGT+CGG	+	chr6.3:8608784-8608803	MS.gene86085:CDS	30.0%
	TCATATCAAATGGTCCATTA+GGG	-	chr6.3:8608889-8608908	None:intergenic	30.0%
	TGCTTCTTCGAAATAATCAT+CGG	-	chr6.3:8608855-8608874	None:intergenic	30.0%
	TTCATATCAAATGGTCCATT+AGG	-	chr6.3:8608890-8608909	None:intergenic	30.0%
	TTCATCATCAATGTCATCAT+CGG	-	chr6.3:8608633-8608652	None:intergenic	30.0%
!	AATCTTTTAGTACCACTACT+AGG	-	chr6.3:8608668-8608687	None:intergenic	30.0%
!	TAAAAGCTGGACAAAAGTTT+TGG	+	chr6.3:8608363-8608382	MS.gene86085:CDS	30.0%
!	TGGCTTGTAAGGAAATTTAT+TGG	+	chr6.3:8608602-8608621	MS.gene86085:CDS	30.0%
!!!	GGAGACAATGTTTTATTTGT+GGG	+	chr6.3:8608758-8608777	MS.gene86085:CDS	30.0%
	AGCATTGCAATTTCGAACAT+TGG	+	chr6.3:8608942-8608961	MS.gene86085:CDS	35.0%
	TCATCATCAATGTCATCATC+GGG	-	chr6.3:8608632-8608651	None:intergenic	35.0%
	TGTCTTGCTTTCATAAAAGC+TGG	+	chr6.3:8608350-8608369	MS.gene86085:CDS	35.0%
	TTGGAATTGGATCTTCAAAG+TGG	+	chr6.3:8608701-8608720	MS.gene86085:CDS	35.0%
!	CGAAGTGTATAAGTTGGAAT+TGG	+	chr6.3:8608688-8608707	MS.gene86085:CDS	35.0%
!	TGATTATTTCGAAGAAGCAC+CGG	+	chr6.3:8608856-8608875	MS.gene86085:CDS	35.0%
!!	ATTTCGAACATTGGTTCACA+CGG	+	chr6.3:8608951-8608970	MS.gene86085:CDS	35.0%
!!	TCCTAATGTTGTTTCACCAT+GGG	+	chr6.3:8608526-8608545	MS.gene86085:CDS	35.0%
!!	TTCCTAATGTTGTTTCACCA+TGG	+	chr6.3:8608525-8608544	MS.gene86085:CDS	35.0%
!!!	GGGAGACAATGTTTTATTTG+TGG	+	chr6.3:8608757-8608776	MS.gene86085:CDS	35.0%
	GCAACTACAACATAATCGTG+AGG	-	chr6.3:8608311-8608330	None:intergenic	40.0%
	TATCGGCAAATCCTACAATC+AGG	+	chr6.3:8608285-8608304	MS.gene86085:CDS	40.0%
	TCAAATGGTCCATTAGGGTA+AGG	-	chr6.3:8608884-8608903	None:intergenic	40.0%
!	GATAGTAACTTGCCTAGTAG+TGG	+	chr6.3:8608653-8608672	MS.gene86085:CDS	40.0%
!!	CCTAATGTTGTTTCACCATG+GGG	+	chr6.3:8608527-8608546	MS.gene86085:CDS	40.0%
!!	CTAATGTTGTTTCACCATGG+GGG	+	chr6.3:8608528-8608547	MS.gene86085:CDS	40.0%
!!	TAAAGGGTTAGTCTATGCTG+CGG	+	chr6.3:8608433-8608452	MS.gene86085:CDS	40.0%
!!	TAATGTTGTTTCACCATGGG+GGG	+	chr6.3:8608529-8608548	MS.gene86085:CDS	40.0%
!!!	TCTATGTCGGCTTCGTATTT+TGG	+	chr6.3:8608797-8608816	MS.gene86085:CDS	40.0%
	AATCATTGGAGGGAGACTTG+TGG	+	chr6.3:8608582-8608601	MS.gene86085:CDS	45.0%
	CCCCATGGTGAAACAACATT+AGG	-	chr6.3:8608530-8608549	None:intergenic	45.0%
	TAATCGTGAGGCCTGATTGT+AGG	-	chr6.3:8608299-8608318	None:intergenic	45.0%
!!	AAAGGGTTAGTCTATGCTGC+GGG	+	chr6.3:8608434-8608453	MS.gene86085:CDS	45.0%
	GGTCCATTAGGGTAAGGATC+CGG	-	chr6.3:8608878-8608897	None:intergenic	50.0%
	GGTTCTTCCGCCATTTCAGT+GGG	+	chr6.3:8608991-8609010	MS.gene86085:CDS	50.0%
	TGAGCAAAATCATCCCCCCA+TGG	-	chr6.3:8608545-8608564	None:intergenic	50.0%
!	AGGGAGACTTGTGGCTTGTA+AGG	+	chr6.3:8608591-8608610	MS.gene86085:CDS	50.0%
	GCACCGGATCCTTACCCTAA+TGG	+	chr6.3:8608872-8608891	MS.gene86085:CDS	55.0%
	GGGTTCTTCCGCCATTTCAG+TGG	+	chr6.3:8608990-8609009	MS.gene86085:CDS	55.0%
	GTTCACACGGATGCCACCTT+CGG	+	chr6.3:8608964-8608983	MS.gene86085:CDS	55.0%
	TAGTCTATGCTGCGGGACGA+TGG	+	chr6.3:8608441-8608460	MS.gene86085:CDS	55.0%
	GGAAGAACCCACACCGAAGG+TGG	-	chr6.3:8608980-8608999	None:intergenic	60.0%
!	ACGGATGCCACCTTCGGTGT+GGG	+	chr6.3:8608970-8608989	MS.gene86085:CDS	60.0%
	CACGGATGCCACCTTCGGTG+TGG	+	chr6.3:8608969-8608988	MS.gene86085:CDS	65.0%
	GGCGGAAGAACCCACACCGA+AGG	-	chr6.3:8608983-8609002	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	8608227	8609018	8608227	ID=MS.gene86085
chr6.3	mRNA	8608227	8609018	8608227	ID=MS.gene86085.t1;Parent=MS.gene86085
chr6.3	exon	8608227	8609018	8608227	ID=MS.gene86085.t1.exon1;Parent=MS.gene86085.t1
chr6.3	CDS	8608227	8609018	8608227	ID=cds.MS.gene86085.t1;Parent=MS.gene86085.t1

Gene Sequence

>MS.gene86085

ATGCCAGATATATATAGAAGAGTTAGATATTATGAATGTAACGTTCATAAGGTTATTTTATCGGCAAATCCTACAATCAGGCCTCACGATTATGTTGTAGTTGCAATTTATGGTATGCGTAAATGTCTTGCTTTCATAAAAGCTGGACAAAAGTTTTGGACTTACGTTGATGACGACTATTTTTGCTTCAGCGATGTTATATTTTATAAAGGGTTAGTCTATGCTGCGGGACGATGGAATAACATTGTCTCTTTTGATATTTGTAACTCGAAGGATTCTTTTGATTGTGAGAAGATCATTCCTAATGTTGTTTCACCATGGGGGGATGATTTTGCTCATCGAGCATATTTTGTGAAATCATTGGAGGGAGACTTGTGGCTTGTAAGGAAATTTATTGGTTTTCCCGATGATGACATTGATGATGAAGATAGTAACTTGCCTAGTAGTGGTACTAAAAGATTCGAAGTGTATAAGTTGGAATTGGATCTTCAAAGTGGTAAGCTTATACAAATGTTAAAGCTTGATAGTTTGGGAGACAATGTTTTATTTGTGGGTGATAGTGATTCTGTATCTATGTCGGCTTCGTATTTTGGTAATTATCTACAAAAGGATTCAATTTATTACACCGATGATTATTTCGAAGAAGCACCGGATCCTTACCCTAATGGACCATTTGATATGAAAATCTATAATGTAAAAGATGGAAACTTTAGTAAGCATTGCAATTTCGAACATTGGTTCACACGGATGCCACCTTCGGTGTGGGTTCTTCCGCCATTTCAGTGGGAATAA

Protein sequence

>MS.gene86085.t1

MPDIYRRVRYYECNVHKVILSANPTIRPHDYVVVAIYGMRKCLAFIKAGQKFWTYVDDDYFCFSDVIFYKGLVYAAGRWNNIVSFDICNSKDSFDCEKIIPNVVSPWGDDFAHRAYFVKSLEGDLWLVRKFIGFPDDDIDDEDSNLPSSGTKRFEVYKLELDLQSGKLIQMLKLDSLGDNVLFVGDSDSVSMSASYFGNYLQKDSIYYTDDYFEEAPDPYPNGPFDMKIYNVKDGNFSKHCNFEHWFTRMPPSVWVLPPFQWE