AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43191

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43191.t1 MTR_7g077620 67.204 372 119 3 1 372 1 369 3.50e-169 478
MS.gene43191.t1 MTR_7g077650 64.042 381 122 6 1 375 1 372 4.56e-158 449
MS.gene43191.t1 MTR_7g077660 63.926 377 125 5 1 375 1 368 1.83e-153 437
MS.gene43191.t1 MTR_7g076110 55.219 297 122 6 86 375 21 313 4.81e-99 296
MS.gene43191.t1 MTR_7g076100 63.713 237 84 2 139 375 66 300 3.88e-94 283
MS.gene43191.t1 MTR_7g076100 78.000 50 11 0 4 53 5 54 2.20e-20 90.9
MS.gene43191.t1 MTR_1g108740 45.288 382 162 16 1 373 1 344 9.96e-85 261
MS.gene43191.t1 MTR_5g038260 39.801 402 191 15 3 375 4 383 6.72e-76 239
MS.gene43191.t1 MTR_5g082770 46.474 312 131 8 5 307 4 288 2.50e-73 230
MS.gene43191.t1 MTR_5g038230 38.734 395 171 15 5 373 4 353 1.31e-70 225
MS.gene43191.t1 MTR_1g064570 38.333 420 191 16 3 375 2 400 1.04e-66 216
MS.gene43191.t1 MTR_7g076130 69.600 125 38 0 168 292 24 148 5.69e-54 175
MS.gene43191.t1 MTR_1g016150 49.474 190 88 6 1 185 1 187 2.27e-47 161
MS.gene43191.t1 MTR_1g016150 61.538 65 19 2 257 321 177 235 9.99e-13 67.8
MS.gene43191.t1 MTR_5g082780 38.298 235 109 9 120 340 6 218 9.48e-34 125
MS.gene43191.t1 MTR_1g108750 43.949 157 51 5 5 160 4 124 2.85e-22 91.3
MS.gene43191.t1 MTR_3g114970 24.021 383 210 17 1 352 1 333 5.22e-13 69.7
MS.gene43191.t1 MTR_3g114990 23.929 397 221 16 3 352 21 383 3.22e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCAAGCCAATCATTT+TGG 0.214143 7.2:-78575365 MS.gene43191:CDS
TTCTCATAGTAAGTAAAATT+AGG 0.229231 7.2:+78575210 None:intergenic
AGGAGGATTATTGAACTTAA+AGG 0.273304 7.2:+78575251 None:intergenic
AATGAACAAACTTGAGAGTT+TGG 0.291432 7.2:-78575004 MS.gene43191:CDS
TACTCACTTGGGCTTGTAAT+CGG 0.312123 7.2:+78575429 None:intergenic
AAGAACACATAGATCATGTT+AGG 0.348741 7.2:-78575815 MS.gene43191:CDS
TTCGAGAGGTGGCAAACTAA+TGG 0.352493 7.2:+78575482 None:intergenic
GATCTTTACCCCGTCCCTTC+AGG 0.356454 7.2:-78574819 MS.gene43191:CDS
AACGACCTTCACCATAGCTT+TGG 0.364212 7.2:+78575157 None:intergenic
CAAGAACATGAGTGTGTGTT+TGG 0.364316 7.2:-78575586 MS.gene43191:CDS
AGAAGCCTATCCTCTTAGTT+TGG 0.365278 7.2:-78575853 MS.gene43191:CDS
TGATGTTTACAAATTAAAAC+TGG 0.368827 7.2:-78575052 MS.gene43191:CDS
CAAGGATGGGATAACTGAAT+AGG 0.377070 7.2:+78575612 None:intergenic
TAGCAATGGGGAAGTTAGAT+TGG 0.377310 7.2:-78575884 None:intergenic
TCTCATAGTAAGTAAAATTA+GGG 0.387493 7.2:+78575211 None:intergenic
TCGAGAGGTGGCAAACTAAT+GGG 0.396706 7.2:+78575483 None:intergenic
CACATAGATCATGTTAGGTT+TGG 0.400301 7.2:-78575810 MS.gene43191:CDS
GGGTTCTACCACAATTTCAA+TGG 0.408660 7.2:-78574780 MS.gene43191:CDS
ACGACCTTCACCATAGCTTT+GGG 0.423915 7.2:+78575158 None:intergenic
CGCCTCTGCTTTCTCCAAGA+TGG 0.446470 7.2:-78574932 MS.gene43191:CDS
ACTCACTTGGGCTTGTAATC+GGG 0.449180 7.2:+78575430 None:intergenic
AAGGATGGGATAACTGAATA+GGG 0.462191 7.2:+78575613 None:intergenic
TTGGCGTGGTGTGCAAGAAT+TGG 0.475029 7.2:-78575791 MS.gene43191:CDS
GGTTCTACCACAATTTCAAT+GGG 0.480142 7.2:-78574779 MS.gene43191:CDS
CTGGTATTTGCTGACAACCT+TGG 0.497796 7.2:-78575288 MS.gene43191:CDS
AGCAATGGGGAAGTTAGATT+GGG 0.499131 7.2:-78575883 None:intergenic
AAGAACATGAGTGTGTGTTT+GGG 0.500810 7.2:-78575585 MS.gene43191:CDS
TCATGTTCTTGATGCAAGGA+TGG 0.501083 7.2:+78575598 None:intergenic
TAGTTTGGTACTTGATAAGT+TGG 0.501505 7.2:-78575838 MS.gene43191:CDS
GTGTGTTTGGGCTTTAGCCA+TGG 0.507415 7.2:-78575573 MS.gene43191:CDS
TAGCATCTTCCTGAAGGGAC+GGG 0.508712 7.2:+78574810 None:intergenic
CTACACGGATCCAAAATGAT+TGG 0.509049 7.2:+78575355 None:intergenic
CCTTCACCATAGCTTTGGGC+TGG 0.509210 7.2:+78575162 None:intergenic
GTTTGGGCTTTAGCCATGGT+TGG 0.511722 7.2:-78575569 MS.gene43191:CDS
TTATCAAGTACCAAACTAAG+AGG 0.513488 7.2:+78575843 None:intergenic
AATCGTTGAATGGAGACATA+TGG 0.523073 7.2:-78575125 MS.gene43191:CDS
CATATCTATAATGCGGAGGA+TGG 0.531607 7.2:-78574856 MS.gene43191:CDS
AGGATACAATGGTGCGTCCA+AGG 0.537916 7.2:+78575271 None:intergenic
GCTGCAACCACATACTCACT+TGG 0.539884 7.2:+78575417 None:intergenic
GTTGATGAAAGTGATAAAGA+TGG 0.555094 7.2:-78574889 MS.gene43191:CDS
AAAATGTAGCATCTTCCTGA+AGG 0.561599 7.2:+78574804 None:intergenic
AAGTACCAAACTAAGAGGAT+AGG 0.568196 7.2:+78575848 None:intergenic
AGTGTTCCAGCCCAAAGCTA+TGG 0.568256 7.2:-78575168 MS.gene43191:CDS
GTAGCATCTTCCTGAAGGGA+CGG 0.568660 7.2:+78574809 None:intergenic
TACAAGCCCAAGTGAGTATG+TGG 0.569105 7.2:-78575424 MS.gene43191:CDS
GGTCAATCCCATTGAAATTG+TGG 0.574786 7.2:+78574772 None:intergenic
AGCATCTTCCTGAAGGGACG+GGG 0.577537 7.2:+78574811 None:intergenic
ACCTCTCGAAAATATCAAGA+AGG 0.578390 7.2:-78575469 MS.gene43191:CDS
TGTAATCGGGTCAGCAGACA+AGG 0.580068 7.2:+78575443 None:intergenic
TACGATAATTTATGTATGAG+AGG 0.585202 7.2:+78575700 None:intergenic
ACACTCATGTTCTTGATGCA+AGG 0.588895 7.2:+78575594 None:intergenic
ATTGAACTTAAAGGATACAA+TGG 0.590271 7.2:+78575260 None:intergenic
CATGTTCTTGATGCAAGGAT+GGG 0.593302 7.2:+78575599 None:intergenic
GAGAGGATCATCAACAAAGG+TGG 0.600429 7.2:+78575717 None:intergenic
GTTGCAGCGATTTACAATTG+TGG 0.603650 7.2:-78575402 MS.gene43191:CDS
AGAGGATCATCAACAAAGGT+GGG 0.609595 7.2:+78575718 None:intergenic
AAATGTAGCATCTTCCTGAA+GGG 0.613321 7.2:+78574805 None:intergenic
TTCCATCTTGGAGAAAGCAG+AGG 0.613967 7.2:+78574930 None:intergenic
CTGCAACCACATACTCACTT+GGG 0.622251 7.2:+78575418 None:intergenic
AGATCATGTTAGGTTTGGCG+TGG 0.628602 7.2:-78575805 MS.gene43191:CDS
CCAGCCCAAAGCTATGGTGA+AGG 0.629782 7.2:-78575162 MS.gene43191:CDS
CATCTTGGAGAAAGCAGAGG+CGG 0.635809 7.2:+78574933 None:intergenic
GGGTCATTTGAGTCATCACA+AGG 0.644746 7.2:+78575231 None:intergenic
TGAGTTACATATCTATAATG+CGG 0.657813 7.2:-78574863 MS.gene43191:CDS
GTGTAGACATAAATGAATTG+TGG 0.661724 7.2:-78575338 MS.gene43191:CDS
ACAATTCATTTATGTCTACA+CGG 0.669650 7.2:+78575340 None:intergenic
GTTACATATCTATAATGCGG+AGG 0.671372 7.2:-78574860 MS.gene43191:CDS
TTAAAACTGGATGCCCAGAG+TGG 0.678752 7.2:-78575039 MS.gene43191:CDS
TATGAGAGGATCATCAACAA+AGG 0.683539 7.2:+78575714 None:intergenic
GTTGAATGGAGACATATGGA+TGG 0.713125 7.2:-78575121 MS.gene43191:CDS
TCATTTGAGTCATCACAAGG+AGG 0.722989 7.2:+78575234 None:intergenic
GTAAACATCAAAATTATACG+AGG 0.728568 7.2:+78575065 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCATAAAAATCATCAATTT+AGG - chr7.2:78574904-78574923 MS.gene43191:CDS 20.0%
!! TCTCATAGTAAGTAAAATTA+GGG + chr7.2:78575445-78575464 None:intergenic 20.0%
!! TGATGTTTACAAATTAAAAC+TGG - chr7.2:78575601-78575620 MS.gene43191:CDS 20.0%
!! TTCTCATAGTAAGTAAAATT+AGG + chr7.2:78575446-78575465 None:intergenic 20.0%
!!! ACGAATGTTGTATTTTATAA+AGG - chr7.2:78575341-78575360 MS.gene43191:CDS 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG + chr7.2:78574908-78574927 None:intergenic 20.0%
! AAAAAGTTTCACTAGTGTTA+TGG + chr7.2:78575135-78575154 None:intergenic 25.0%
! ACAATTCATTTATGTCTACA+CGG + chr7.2:78575316-78575335 None:intergenic 25.0%
! ATTGAACTTAAAGGATACAA+TGG + chr7.2:78575396-78575415 None:intergenic 25.0%
! GTAAACATCAAAATTATACG+AGG + chr7.2:78575591-78575610 None:intergenic 25.0%
! TACGATAATTTATGTATGAG+AGG + chr7.2:78574956-78574975 None:intergenic 25.0%
! TGAGTTACATATCTATAATG+CGG - chr7.2:78575790-78575809 MS.gene43191:CDS 25.0%
!! AATTGAATTCTTTTCCATCT+TGG + chr7.2:78575738-78575757 None:intergenic 25.0%
!! TATTTTGTGAAATCGTTGAA+TGG - chr7.2:78575518-78575537 MS.gene43191:CDS 25.0%
!! TGTTGTATTTTATAAAGGTC+TGG - chr7.2:78575346-78575365 MS.gene43191:CDS 25.0%
!!! ATTTTATTTGTCGGAAACTT+TGG - chr7.2:78575680-78575699 MS.gene43191:CDS 25.0%
!!! GAAGACAACATTTTATTTGT+CGG - chr7.2:78575671-78575690 MS.gene43191:CDS 25.0%
AAGAACACATAGATCATGTT+AGG - chr7.2:78574838-78574857 MS.gene43191:CDS 30.0%
AATGAACAAACTTGAGAGTT+TGG - chr7.2:78575649-78575668 MS.gene43191:CDS 30.0%
AGGAGGATTATTGAACTTAA+AGG + chr7.2:78575405-78575424 None:intergenic 30.0%
GTGTAGACATAAATGAATTG+TGG - chr7.2:78575315-78575334 MS.gene43191:CDS 30.0%
TAGTTTGGTACTTGATAAGT+TGG - chr7.2:78574815-78574834 MS.gene43191:CDS 30.0%
TTATCAAGTACCAAACTAAG+AGG + chr7.2:78574813-78574832 None:intergenic 30.0%
! GTTGATGAAAGTGATAAAGA+TGG - chr7.2:78575764-78575783 MS.gene43191:CDS 30.0%
AAAATGTAGCATCTTCCTGA+AGG + chr7.2:78575852-78575871 None:intergenic 35.0%
AAATGTAGCATCTTCCTGAA+GGG + chr7.2:78575851-78575870 None:intergenic 35.0%
AAGAACATGAGTGTGTGTTT+GGG - chr7.2:78575068-78575087 MS.gene43191:CDS 35.0%
AAGGATGGGATAACTGAATA+GGG + chr7.2:78575043-78575062 None:intergenic 35.0%
AAGTACCAAACTAAGAGGAT+AGG + chr7.2:78574808-78574827 None:intergenic 35.0%
AATCGTTGAATGGAGACATA+TGG - chr7.2:78575528-78575547 MS.gene43191:CDS 35.0%
ACCTCTCGAAAATATCAAGA+AGG - chr7.2:78575184-78575203 MS.gene43191:CDS 35.0%
CACATAGATCATGTTAGGTT+TGG - chr7.2:78574843-78574862 MS.gene43191:CDS 35.0%
GGTTCTACCACAATTTCAAT+GGG - chr7.2:78575874-78575893 MS.gene43191:CDS 35.0%
GTTACATATCTATAATGCGG+AGG - chr7.2:78575793-78575812 MS.gene43191:CDS 35.0%
TATGAGAGGATCATCAACAA+AGG + chr7.2:78574942-78574961 None:intergenic 35.0%
! AAAGAGCAAGCCAATCATTT+TGG - chr7.2:78575288-78575307 MS.gene43191:CDS 35.0%
! CAGGAAGATGCTACATTTTT+GGG - chr7.2:78575853-78575872 MS.gene43191:CDS 35.0%
! TCAGGAAGATGCTACATTTT+TGG - chr7.2:78575852-78575871 MS.gene43191:CDS 35.0%
!! ACCTTCTTGATATTTTCGAG+AGG + chr7.2:78575188-78575207 None:intergenic 35.0%
!! ATTTTAATGCAAGCCAACCA+TGG + chr7.2:78575100-78575119 None:intergenic 35.0%
!! TTCTTGATATTTTCGAGAGG+TGG + chr7.2:78575185-78575204 None:intergenic 35.0%
!! TTGTTCAAGTTTTCCACTCT+GGG + chr7.2:78575630-78575649 None:intergenic 35.0%
!! TTTGTTCAAGTTTTCCACTC+TGG + chr7.2:78575631-78575650 None:intergenic 35.0%
ACACTCATGTTCTTGATGCA+AGG + chr7.2:78575062-78575081 None:intergenic 40.0%
AGAAGCCTATCCTCTTAGTT+TGG - chr7.2:78574800-78574819 MS.gene43191:CDS 40.0%
AGAGGATCATCAACAAAGGT+GGG + chr7.2:78574938-78574957 None:intergenic 40.0%
CAAGAACATGAGTGTGTGTT+TGG - chr7.2:78575067-78575086 MS.gene43191:CDS 40.0%
CAAGGATGGGATAACTGAAT+AGG + chr7.2:78575044-78575063 None:intergenic 40.0%
CATATCTATAATGCGGAGGA+TGG - chr7.2:78575797-78575816 MS.gene43191:CDS 40.0%
CATGTTCTTGATGCAAGGAT+GGG + chr7.2:78575057-78575076 None:intergenic 40.0%
CTACACGGATCCAAAATGAT+TGG + chr7.2:78575301-78575320 None:intergenic 40.0%
GGGTTCTACCACAATTTCAA+TGG - chr7.2:78575873-78575892 MS.gene43191:CDS 40.0%
GTTGAATGGAGACATATGGA+TGG - chr7.2:78575532-78575551 MS.gene43191:CDS 40.0%
GTTGCAGCGATTTACAATTG+TGG - chr7.2:78575251-78575270 MS.gene43191:CDS 40.0%
TACTCACTTGGGCTTGTAAT+CGG + chr7.2:78575227-78575246 None:intergenic 40.0%
TCATGTTCTTGATGCAAGGA+TGG + chr7.2:78575058-78575077 None:intergenic 40.0%
TCATTTGAGTCATCACAAGG+AGG + chr7.2:78575422-78575441 None:intergenic 40.0%
AACGACCTTCACCATAGCTT+TGG + chr7.2:78575499-78575518 None:intergenic 45.0%
ACGACCTTCACCATAGCTTT+GGG + chr7.2:78575498-78575517 None:intergenic 45.0%
ACTCACTTGGGCTTGTAATC+GGG + chr7.2:78575226-78575245 None:intergenic 45.0%
AGATCATGTTAGGTTTGGCG+TGG - chr7.2:78574848-78574867 MS.gene43191:CDS 45.0%
CTGCAACCACATACTCACTT+GGG + chr7.2:78575238-78575257 None:intergenic 45.0%
GAGAGGATCATCAACAAAGG+TGG + chr7.2:78574939-78574958 None:intergenic 45.0%
GGGTCATTTGAGTCATCACA+AGG + chr7.2:78575425-78575444 None:intergenic 45.0%
TACAAGCCCAAGTGAGTATG+TGG - chr7.2:78575229-78575248 MS.gene43191:CDS 45.0%
TCGAGAGGTGGCAAACTAAT+GGG + chr7.2:78575173-78575192 None:intergenic 45.0%
TTAAAACTGGATGCCCAGAG+TGG - chr7.2:78575614-78575633 MS.gene43191:CDS 45.0%
TTCCATCTTGGAGAAAGCAG+AGG + chr7.2:78575726-78575745 None:intergenic 45.0%
TTCGAGAGGTGGCAAACTAA+TGG + chr7.2:78575174-78575193 None:intergenic 45.0%
! CTGGTATTTGCTGACAACCT+TGG - chr7.2:78575365-78575384 MS.gene43191:CDS 45.0%
AGGATACAATGGTGCGTCCA+AGG + chr7.2:78575385-78575404 None:intergenic 50.0%
AGTGTTCCAGCCCAAAGCTA+TGG - chr7.2:78575485-78575504 MS.gene43191:CDS 50.0%
CATCTTGGAGAAAGCAGAGG+CGG + chr7.2:78575723-78575742 None:intergenic 50.0%
GCTGCAACCACATACTCACT+TGG + chr7.2:78575239-78575258 None:intergenic 50.0%
GTAGCATCTTCCTGAAGGGA+CGG + chr7.2:78575847-78575866 None:intergenic 50.0%
TAGCATCTTCCTGAAGGGAC+GGG + chr7.2:78575846-78575865 None:intergenic 50.0%
TGTAATCGGGTCAGCAGACA+AGG + chr7.2:78575213-78575232 None:intergenic 50.0%
TTGGCGTGGTGTGCAAGAAT+TGG - chr7.2:78574862-78574881 MS.gene43191:CDS 50.0%
! GTGTGTTTGGGCTTTAGCCA+TGG - chr7.2:78575080-78575099 MS.gene43191:CDS 50.0%
! GTTTGGGCTTTAGCCATGGT+TGG - chr7.2:78575084-78575103 MS.gene43191:CDS 50.0%
AGCATCTTCCTGAAGGGACG+GGG + chr7.2:78575845-78575864 None:intergenic 55.0%
CCAGCCCAAAGCTATGGTGA+AGG - chr7.2:78575491-78575510 MS.gene43191:CDS 55.0%
CGCCTCTGCTTTCTCCAAGA+TGG - chr7.2:78575721-78575740 MS.gene43191:CDS 55.0%
GATCTTTACCCCGTCCCTTC+AGG - chr7.2:78575834-78575853 MS.gene43191:CDS 55.0%
!! CCTTCACCATAGCTTTGGGC+TGG + chr7.2:78575494-78575513 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 78574774 78575901 78574774 ID=MS.gene43191
chr7.2 mRNA 78574774 78575901 78574774 ID=MS.gene43191.t1;Parent=MS.gene43191
chr7.2 exon 78574774 78575901 78574774 ID=MS.gene43191.t1.exon1;Parent=MS.gene43191.t1
chr7.2 CDS 78574774 78575901 78574774 ID=cds.MS.gene43191.t1;Parent=MS.gene43191.t1
Gene Sequence

>MS.gene43191

ATGGGGAAGTTAGATTGGGCTAATTTAGAAGCCTATCCTCTTAGTTTGGTACTTGATAAGTTGGAAGAACACATAGATCATGTTAGGTTTGGCGTGGTGTGCAAGAATTGGCTATCAATTGCAAAGCTTAACCATAAAAATCATCAATTTAGGATTAATGTCCCACCTTTGTTGATGATCCTCTCATACATAAATTATCGTATGCTTATAATATCACAGACGAAGAAGAGATATTTGTTGTATAGTATTCTTAGTGAGAAAGAGTACCCTATTCAGTTATCCCATCCTTGCATCAAGAACATGAGTGTGTGTTTGGGCTTTAGCCATGGTTGGCTTGCATTAAAATATGATAACAAAGCCATAACACTAGTGAAACTTTTTAAGTATAGTGTAGCTCCCATTAGTTTGCCACCTCTCGAAAATATCAAGAAGGTTACCTTGTCTGCTGACCCGATTACAAGCCCAAGTGAGTATGTGGTTGCAGCGATTTACAATTGTGGTCGTCTCGCTTTGAAAAGAGCAAGCCAATCATTTTGGATCCGTGTAGACATAAATGAATTGTGGTGCACGAATGTTGTATTTTATAAAGGTCTGGTATTTGCTGACAACCTTGGACGCACCATTGTATCCTTTAAGTTCAATAATCCTCCTTGTGATGACTCAAATGACCCTAATTTTACTTACTATGAGAAGATAGCTAGCACTAGATATAGTGTTCCAGCCCAAAGCTATGGTGAAGGTCGTTATTTTGTGAAATCGTTGAATGGAGACATATGGATGGTGAGAAGAAATTTAATCGATCAGTATGACAAGTCCTCGTATAATTTTGATGTTTACAAATTAAAACTGGATGCCCAGAGTGGAAAACTTGAACAAATGAACAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAAACTTTGGTGATTCAATTTCTTTGTCCGCCTCTGCTTTCTCCAAGATGGAAAAGAATTCAATTTATTTTGTTGATGAAAGTGATAAAGATGGTATTGAGTTACATATCTATAATGCGGAGGATGGAAGTGTTGACAGTCGATCTTTACCCCGTCCCTTCAGGAAGATGCTACATTTTTGGGTTCTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene43191.t1

MGKLDWANLEAYPLSLVLDKLEEHIDHVRFGVVCKNWLSIAKLNHKNHQFRINVPPLLMILSYINYRMLIISQTKKRYLLYSILSEKEYPIQLSHPCIKNMSVCLGFSHGWLALKYDNKAITLVKLFKYSVAPISLPPLENIKKVTLSADPITSPSEYVVAAIYNCGRLALKRASQSFWIRVDINELWCTNVVFYKGLVFADNLGRTIVSFKFNNPPCDDSNDPNFTYYEKIASTRYSVPAQSYGEGRYFVKSLNGDIWMVRRNLIDQYDKSSYNFDVYKLKLDAQSGKLEQMNKLESLEDNILFVGNFGDSISLSASAFSKMEKNSIYFVDESDKDGIELHIYNAEDGSVDSRSLPRPFRKMLHFWVLPQFQWD