AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95450.t1 MTR_7g077620 67.204 372 119 3 1 372 1 369 3.50e-169 478
MS.gene95450.t1 MTR_7g077650 64.042 381 122 6 1 375 1 372 4.56e-158 449
MS.gene95450.t1 MTR_7g077660 63.926 377 125 5 1 375 1 368 1.83e-153 437
MS.gene95450.t1 MTR_7g076110 55.219 297 122 6 86 375 21 313 4.81e-99 296
MS.gene95450.t1 MTR_7g076100 63.713 237 84 2 139 375 66 300 3.88e-94 283
MS.gene95450.t1 MTR_7g076100 78.000 50 11 0 4 53 5 54 2.20e-20 90.9
MS.gene95450.t1 MTR_1g108740 45.288 382 162 16 1 373 1 344 9.96e-85 261
MS.gene95450.t1 MTR_5g038260 39.801 402 191 15 3 375 4 383 6.72e-76 239
MS.gene95450.t1 MTR_5g082770 46.474 312 131 8 5 307 4 288 2.50e-73 230
MS.gene95450.t1 MTR_5g038230 38.734 395 171 15 5 373 4 353 1.31e-70 225
MS.gene95450.t1 MTR_1g064570 38.333 420 191 16 3 375 2 400 1.04e-66 216
MS.gene95450.t1 MTR_7g076130 69.600 125 38 0 168 292 24 148 5.69e-54 175
MS.gene95450.t1 MTR_1g016150 49.474 190 88 6 1 185 1 187 2.27e-47 161
MS.gene95450.t1 MTR_1g016150 61.538 65 19 2 257 321 177 235 9.99e-13 67.8
MS.gene95450.t1 MTR_5g082780 38.298 235 109 9 120 340 6 218 9.48e-34 125
MS.gene95450.t1 MTR_1g108750 43.949 157 51 5 5 160 4 124 2.85e-22 91.3
MS.gene95450.t1 MTR_3g114970 24.021 383 210 17 1 352 1 333 5.22e-13 69.7
MS.gene95450.t1 MTR_3g114990 23.929 397 221 16 3 352 21 383 3.22e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGCAAGCCAATCATTT+TGG 0.214143 7.4:-80870022 MS.gene95450:CDS
TTCTCATAGTAAGTAAAATT+AGG 0.229231 7.4:+80869867 None:intergenic
AGGAGGATTATTGAACTTAA+AGG 0.273304 7.4:+80869908 None:intergenic
AATGAACAAACTTGAGAGTT+TGG 0.291432 7.4:-80869661 MS.gene95450:CDS
TACTCACTTGGGCTTGTAAT+CGG 0.312123 7.4:+80870086 None:intergenic
AAGAACACATAGATCATGTT+AGG 0.348741 7.4:-80870472 MS.gene95450:CDS
TTCGAGAGGTGGCAAACTAA+TGG 0.352493 7.4:+80870139 None:intergenic
GATCTTTACCCCGTCCCTTC+AGG 0.356454 7.4:-80869476 MS.gene95450:CDS
AACGACCTTCACCATAGCTT+TGG 0.364212 7.4:+80869814 None:intergenic
CAAGAACATGAGTGTGTGTT+TGG 0.364316 7.4:-80870243 MS.gene95450:CDS
AGAAGCCTATCCTCTTAGTT+TGG 0.365278 7.4:-80870510 MS.gene95450:CDS
TGATGTTTACAAATTAAAAC+TGG 0.368827 7.4:-80869709 MS.gene95450:CDS
CAAGGATGGGATAACTGAAT+AGG 0.377070 7.4:+80870269 None:intergenic
TAGCAATGGGGAAGTTAGAT+TGG 0.377310 7.4:-80870541 None:intergenic
TCTCATAGTAAGTAAAATTA+GGG 0.387493 7.4:+80869868 None:intergenic
TCGAGAGGTGGCAAACTAAT+GGG 0.396706 7.4:+80870140 None:intergenic
CACATAGATCATGTTAGGTT+TGG 0.400301 7.4:-80870467 MS.gene95450:CDS
GGGTTCTACCACAATTTCAA+TGG 0.408660 7.4:-80869437 MS.gene95450:CDS
ACGACCTTCACCATAGCTTT+GGG 0.423915 7.4:+80869815 None:intergenic
CGCCTCTGCTTTCTCCAAGA+TGG 0.446470 7.4:-80869589 MS.gene95450:CDS
ACTCACTTGGGCTTGTAATC+GGG 0.449180 7.4:+80870087 None:intergenic
AAGGATGGGATAACTGAATA+GGG 0.462191 7.4:+80870270 None:intergenic
TTGGCGTGGTGTGCAAGAAT+TGG 0.475029 7.4:-80870448 MS.gene95450:CDS
GGTTCTACCACAATTTCAAT+GGG 0.480142 7.4:-80869436 MS.gene95450:CDS
CTGGTATTTGCTGACAACCT+TGG 0.497796 7.4:-80869945 MS.gene95450:CDS
AGCAATGGGGAAGTTAGATT+GGG 0.499131 7.4:-80870540 None:intergenic
AAGAACATGAGTGTGTGTTT+GGG 0.500810 7.4:-80870242 MS.gene95450:CDS
TCATGTTCTTGATGCAAGGA+TGG 0.501083 7.4:+80870255 None:intergenic
TAGTTTGGTACTTGATAAGT+TGG 0.501505 7.4:-80870495 MS.gene95450:CDS
GTGTGTTTGGGCTTTAGCCA+TGG 0.507415 7.4:-80870230 MS.gene95450:CDS
TAGCATCTTCCTGAAGGGAC+GGG 0.508712 7.4:+80869467 None:intergenic
CTACACGGATCCAAAATGAT+TGG 0.509049 7.4:+80870012 None:intergenic
CCTTCACCATAGCTTTGGGC+TGG 0.509210 7.4:+80869819 None:intergenic
GTTTGGGCTTTAGCCATGGT+TGG 0.511722 7.4:-80870226 MS.gene95450:CDS
TTATCAAGTACCAAACTAAG+AGG 0.513488 7.4:+80870500 None:intergenic
AATCGTTGAATGGAGACATA+TGG 0.523073 7.4:-80869782 MS.gene95450:CDS
CATATCTATAATGCGGAGGA+TGG 0.531607 7.4:-80869513 MS.gene95450:CDS
AGGATACAATGGTGCGTCCA+AGG 0.537916 7.4:+80869928 None:intergenic
GCTGCAACCACATACTCACT+TGG 0.539884 7.4:+80870074 None:intergenic
GTTGATGAAAGTGATAAAGA+TGG 0.555094 7.4:-80869546 MS.gene95450:CDS
AAAATGTAGCATCTTCCTGA+AGG 0.561599 7.4:+80869461 None:intergenic
AAGTACCAAACTAAGAGGAT+AGG 0.568196 7.4:+80870505 None:intergenic
AGTGTTCCAGCCCAAAGCTA+TGG 0.568256 7.4:-80869825 MS.gene95450:CDS
GTAGCATCTTCCTGAAGGGA+CGG 0.568660 7.4:+80869466 None:intergenic
TACAAGCCCAAGTGAGTATG+TGG 0.569105 7.4:-80870081 MS.gene95450:CDS
GGTCAATCCCATTGAAATTG+TGG 0.574786 7.4:+80869429 None:intergenic
AGCATCTTCCTGAAGGGACG+GGG 0.577537 7.4:+80869468 None:intergenic
ACCTCTCGAAAATATCAAGA+AGG 0.578390 7.4:-80870126 MS.gene95450:CDS
TGTAATCGGGTCAGCAGACA+AGG 0.580068 7.4:+80870100 None:intergenic
TACGATAATTTATGTATGAG+AGG 0.585202 7.4:+80870357 None:intergenic
ACACTCATGTTCTTGATGCA+AGG 0.588895 7.4:+80870251 None:intergenic
ATTGAACTTAAAGGATACAA+TGG 0.590271 7.4:+80869917 None:intergenic
CATGTTCTTGATGCAAGGAT+GGG 0.593302 7.4:+80870256 None:intergenic
GAGAGGATCATCAACAAAGG+TGG 0.600429 7.4:+80870374 None:intergenic
GTTGCAGCGATTTACAATTG+TGG 0.603650 7.4:-80870059 MS.gene95450:CDS
AGAGGATCATCAACAAAGGT+GGG 0.609595 7.4:+80870375 None:intergenic
AAATGTAGCATCTTCCTGAA+GGG 0.613321 7.4:+80869462 None:intergenic
TTCCATCTTGGAGAAAGCAG+AGG 0.613967 7.4:+80869587 None:intergenic
CTGCAACCACATACTCACTT+GGG 0.622251 7.4:+80870075 None:intergenic
AGATCATGTTAGGTTTGGCG+TGG 0.628602 7.4:-80870462 MS.gene95450:CDS
CCAGCCCAAAGCTATGGTGA+AGG 0.629782 7.4:-80869819 MS.gene95450:CDS
CATCTTGGAGAAAGCAGAGG+CGG 0.635809 7.4:+80869590 None:intergenic
GGGTCATTTGAGTCATCACA+AGG 0.644746 7.4:+80869888 None:intergenic
TGAGTTACATATCTATAATG+CGG 0.657813 7.4:-80869520 MS.gene95450:CDS
GTGTAGACATAAATGAATTG+TGG 0.661724 7.4:-80869995 MS.gene95450:CDS
ACAATTCATTTATGTCTACA+CGG 0.669650 7.4:+80869997 None:intergenic
GTTACATATCTATAATGCGG+AGG 0.671372 7.4:-80869517 MS.gene95450:CDS
TTAAAACTGGATGCCCAGAG+TGG 0.678752 7.4:-80869696 MS.gene95450:CDS
TATGAGAGGATCATCAACAA+AGG 0.683539 7.4:+80870371 None:intergenic
GTTGAATGGAGACATATGGA+TGG 0.713125 7.4:-80869778 MS.gene95450:CDS
TCATTTGAGTCATCACAAGG+AGG 0.722989 7.4:+80869891 None:intergenic
GTAAACATCAAAATTATACG+AGG 0.728568 7.4:+80869722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTAATGTTGTATTTTATAA+AGG - chr7.4:80869998-80870017 MS.gene95450:CDS 15.0%
!! ACCATAAAAATCATCAATTT+AGG - chr7.4:80869561-80869580 MS.gene95450:CDS 20.0%
!! TCTCATAGTAAGTAAAATTA+GGG + chr7.4:80870102-80870121 None:intergenic 20.0%
!! TGATGTTTACAAATTAAAAC+TGG - chr7.4:80870258-80870277 MS.gene95450:CDS 20.0%
!! TTCTCATAGTAAGTAAAATT+AGG + chr7.4:80870103-80870122 None:intergenic 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG + chr7.4:80869565-80869584 None:intergenic 20.0%
! AAAAAGTTTCACTAGTGTTA+TGG + chr7.4:80869792-80869811 None:intergenic 25.0%
! ACAATTCATTTATGTCTACA+CGG + chr7.4:80869973-80869992 None:intergenic 25.0%
! ATTGAACTTAAAGGATACAA+TGG + chr7.4:80870053-80870072 None:intergenic 25.0%
! GTAAACATCAAAATTATACG+AGG + chr7.4:80870248-80870267 None:intergenic 25.0%
! TACGATAATTTATGTATGAG+AGG + chr7.4:80869613-80869632 None:intergenic 25.0%
! TGAGTTACATATCTATAATG+CGG - chr7.4:80870447-80870466 MS.gene95450:CDS 25.0%
!! AATTGAATTCTTTTCCATCT+TGG + chr7.4:80870395-80870414 None:intergenic 25.0%
!! TATTTTGTGAAATCGTTGAA+TGG - chr7.4:80870175-80870194 MS.gene95450:CDS 25.0%
!! TGTTGTATTTTATAAAGGTC+TGG - chr7.4:80870003-80870022 MS.gene95450:CDS 25.0%
!!! ATTTTATTTGTCGGAAACTT+TGG - chr7.4:80870337-80870356 MS.gene95450:CDS 25.0%
!!! GAAGACAACATTTTATTTGT+CGG - chr7.4:80870328-80870347 MS.gene95450:CDS 25.0%
AAGAACACATAGATCATGTT+AGG - chr7.4:80869495-80869514 MS.gene95450:CDS 30.0%
AATGAACAAACTTGAGAGTT+TGG - chr7.4:80870306-80870325 MS.gene95450:CDS 30.0%
AGGAGGATTATTGAACTTAA+AGG + chr7.4:80870062-80870081 None:intergenic 30.0%
GTGTAGACATAAATGAATTG+TGG - chr7.4:80869972-80869991 MS.gene95450:CDS 30.0%
TAGTTTGGTACTTGATAAGT+TGG - chr7.4:80869472-80869491 MS.gene95450:CDS 30.0%
TTATCAAGTACCAAACTAAG+AGG + chr7.4:80869470-80869489 None:intergenic 30.0%
! GTTGATGAAAGTGATAAAGA+TGG - chr7.4:80870421-80870440 MS.gene95450:CDS 30.0%
AAAATGTAGCATCTTCCTGA+AGG + chr7.4:80870509-80870528 None:intergenic 35.0%
AAATGTAGCATCTTCCTGAA+GGG + chr7.4:80870508-80870527 None:intergenic 35.0%
AAGAACATGAGTGTGTGTTT+GGG - chr7.4:80869725-80869744 MS.gene95450:CDS 35.0%
AAGGATGGGATAACTGAATA+GGG + chr7.4:80869700-80869719 None:intergenic 35.0%
AAGTACCAAACTAAGAGGAT+AGG + chr7.4:80869465-80869484 None:intergenic 35.0%
AATCGTTGAATGGAGACATA+TGG - chr7.4:80870185-80870204 MS.gene95450:CDS 35.0%
ACCTCTCGAAAATATCAAGA+AGG - chr7.4:80869841-80869860 MS.gene95450:CDS 35.0%
CACATAGATCATGTTAGGTT+TGG - chr7.4:80869500-80869519 MS.gene95450:CDS 35.0%
GGTTCTACCACAATTTCAAT+GGG - chr7.4:80870531-80870550 MS.gene95450:CDS 35.0%
GTTACATATCTATAATGCGG+AGG - chr7.4:80870450-80870469 MS.gene95450:CDS 35.0%
TATGAGAGGATCATCAACAA+AGG + chr7.4:80869599-80869618 None:intergenic 35.0%
! AAAGAGCAAGCCAATCATTT+TGG - chr7.4:80869945-80869964 MS.gene95450:CDS 35.0%
! CAGGAAGATGCTACATTTTT+GGG - chr7.4:80870510-80870529 MS.gene95450:CDS 35.0%
! TCAGGAAGATGCTACATTTT+TGG - chr7.4:80870509-80870528 MS.gene95450:CDS 35.0%
!! ACCTTCTTGATATTTTCGAG+AGG + chr7.4:80869845-80869864 None:intergenic 35.0%
!! ATTTTAATGCAAGCCAACCA+TGG + chr7.4:80869757-80869776 None:intergenic 35.0%
!! TTCTTGATATTTTCGAGAGG+TGG + chr7.4:80869842-80869861 None:intergenic 35.0%
!! TTGTTCAAGTTTTCCACTCT+GGG + chr7.4:80870287-80870306 None:intergenic 35.0%
!! TTTGTTCAAGTTTTCCACTC+TGG + chr7.4:80870288-80870307 None:intergenic 35.0%
ACACTCATGTTCTTGATGCA+AGG + chr7.4:80869719-80869738 None:intergenic 40.0%
AGAAGCCTATCCTCTTAGTT+TGG - chr7.4:80869457-80869476 MS.gene95450:CDS 40.0%
AGAGGATCATCAACAAAGGT+GGG + chr7.4:80869595-80869614 None:intergenic 40.0%
CAAGAACATGAGTGTGTGTT+TGG - chr7.4:80869724-80869743 MS.gene95450:CDS 40.0%
CAAGGATGGGATAACTGAAT+AGG + chr7.4:80869701-80869720 None:intergenic 40.0%
CATATCTATAATGCGGAGGA+TGG - chr7.4:80870454-80870473 MS.gene95450:CDS 40.0%
CATGTTCTTGATGCAAGGAT+GGG + chr7.4:80869714-80869733 None:intergenic 40.0%
CTACACGGATCCAAAATGAT+TGG + chr7.4:80869958-80869977 None:intergenic 40.0%
GGGTTCTACCACAATTTCAA+TGG - chr7.4:80870530-80870549 MS.gene95450:CDS 40.0%
GTTGAATGGAGACATATGGA+TGG - chr7.4:80870189-80870208 MS.gene95450:CDS 40.0%
GTTGCAGCGATTTACAATTG+TGG - chr7.4:80869908-80869927 MS.gene95450:CDS 40.0%
TACTCACTTGGGCTTGTAAT+CGG + chr7.4:80869884-80869903 None:intergenic 40.0%
TCATGTTCTTGATGCAAGGA+TGG + chr7.4:80869715-80869734 None:intergenic 40.0%
TCATTTGAGTCATCACAAGG+AGG + chr7.4:80870079-80870098 None:intergenic 40.0%
AACGACCTTCACCATAGCTT+TGG + chr7.4:80870156-80870175 None:intergenic 45.0%
ACGACCTTCACCATAGCTTT+GGG + chr7.4:80870155-80870174 None:intergenic 45.0%
ACTCACTTGGGCTTGTAATC+GGG + chr7.4:80869883-80869902 None:intergenic 45.0%
AGATCATGTTAGGTTTGGCG+TGG - chr7.4:80869505-80869524 MS.gene95450:CDS 45.0%
CTGCAACCACATACTCACTT+GGG + chr7.4:80869895-80869914 None:intergenic 45.0%
GAGAGGATCATCAACAAAGG+TGG + chr7.4:80869596-80869615 None:intergenic 45.0%
GGGTCATTTGAGTCATCACA+AGG + chr7.4:80870082-80870101 None:intergenic 45.0%
TACAAGCCCAAGTGAGTATG+TGG - chr7.4:80869886-80869905 MS.gene95450:CDS 45.0%
TCGAGAGGTGGCAAACTAAT+GGG + chr7.4:80869830-80869849 None:intergenic 45.0%
TTAAAACTGGATGCCCAGAG+TGG - chr7.4:80870271-80870290 MS.gene95450:CDS 45.0%
TTCCATCTTGGAGAAAGCAG+AGG + chr7.4:80870383-80870402 None:intergenic 45.0%
TTCGAGAGGTGGCAAACTAA+TGG + chr7.4:80869831-80869850 None:intergenic 45.0%
! CTGGTATTTGCTGACAACCT+TGG - chr7.4:80870022-80870041 MS.gene95450:CDS 45.0%
AGGATACAATGGTGCGTCCA+AGG + chr7.4:80870042-80870061 None:intergenic 50.0%
AGTGTTCCAGCCCAAAGCTA+TGG - chr7.4:80870142-80870161 MS.gene95450:CDS 50.0%
CATCTTGGAGAAAGCAGAGG+CGG + chr7.4:80870380-80870399 None:intergenic 50.0%
GCTGCAACCACATACTCACT+TGG + chr7.4:80869896-80869915 None:intergenic 50.0%
GTAGCATCTTCCTGAAGGGA+CGG + chr7.4:80870504-80870523 None:intergenic 50.0%
TAGCATCTTCCTGAAGGGAC+GGG + chr7.4:80870503-80870522 None:intergenic 50.0%
TGTAATCGGGTCAGCAGACA+AGG + chr7.4:80869870-80869889 None:intergenic 50.0%
TTGGCGTGGTGTGCAAGAAT+TGG - chr7.4:80869519-80869538 MS.gene95450:CDS 50.0%
! GTGTGTTTGGGCTTTAGCCA+TGG - chr7.4:80869737-80869756 MS.gene95450:CDS 50.0%
! GTTTGGGCTTTAGCCATGGT+TGG - chr7.4:80869741-80869760 MS.gene95450:CDS 50.0%
AGCATCTTCCTGAAGGGACG+GGG + chr7.4:80870502-80870521 None:intergenic 55.0%
CCAGCCCAAAGCTATGGTGA+AGG - chr7.4:80870148-80870167 MS.gene95450:CDS 55.0%
CGCCTCTGCTTTCTCCAAGA+TGG - chr7.4:80870378-80870397 MS.gene95450:CDS 55.0%
GATCTTTACCCCGTCCCTTC+AGG - chr7.4:80870491-80870510 MS.gene95450:CDS 55.0%
!! CCTTCACCATAGCTTTGGGC+TGG + chr7.4:80870151-80870170 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 80869431 80870558 80869431 ID=MS.gene95450
chr7.4 mRNA 80869431 80870558 80869431 ID=MS.gene95450.t1;Parent=MS.gene95450
chr7.4 exon 80869431 80870558 80869431 ID=MS.gene95450.t1.exon1;Parent=MS.gene95450.t1
chr7.4 CDS 80869431 80870558 80869431 ID=cds.MS.gene95450.t1;Parent=MS.gene95450.t1
Gene Sequence

>MS.gene95450

ATGGGGAAGTTAGATTGGGCTAATTTAGAAGCCTATCCTCTTAGTTTGGTACTTGATAAGTTGGAAGAACACATAGATCATGTTAGGTTTGGCGTGGTGTGCAAGAATTGGCTATCAATTGCAAAGCTTAACCATAAAAATCATCAATTTAGGATTAATGTCCCACCTTTGTTGATGATCCTCTCATACATAAATTATCGTATGCTTATAATATCACAGACGAAGAAGAGATATTTGTTGTATAGTATTCTTAGTGAGAAAGAGTACCCTATTCAGTTATCCCATCCTTGCATCAAGAACATGAGTGTGTGTTTGGGCTTTAGCCATGGTTGGCTTGCATTAAAATATGATAACAAAGCCATAACACTAGTGAAACTTTTTAAGTATAGTGTAGCTCCCATTAGTTTGCCACCTCTCGAAAATATCAAGAAGGTTACCTTGTCTGCTGACCCGATTACAAGCCCAAGTGAGTATGTGGTTGCAGCGATTTACAATTGTGGTCGTCTCGCTTTGAAAAGAGCAAGCCAATCATTTTGGATCCGTGTAGACATAAATGAATTGTGGTGCACTAATGTTGTATTTTATAAAGGTCTGGTATTTGCTGACAACCTTGGACGCACCATTGTATCCTTTAAGTTCAATAATCCTCCTTGTGATGACTCAAATGACCCTAATTTTACTTACTATGAGAAGATAGCTAGCACTAGATATAGTGTTCCAGCCCAAAGCTATGGTGAAGGTCGTTATTTTGTGAAATCGTTGAATGGAGACATATGGATGGTGAGAAGAAATTTAATCGATCAGTATGACAAGTCCTCGTATAATTTTGATGTTTACAAATTAAAACTGGATGCCCAGAGTGGAAAACTTGAACAAATGAACAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAAACTTTGGTGATTCAATTTCTTTGTCCGCCTCTGCTTTCTCCAAGATGGAAAAGAATTCAATTTATTTTGTTGATGAAAGTGATAAAGATGGTATTGAGTTACATATCTATAATGCGGAGGATGGAAGTGTTGACAGTCGATCTTTACCCCGTCCCTTCAGGAAGATGCTACATTTTTGGGTTCTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene95450.t1

MGKLDWANLEAYPLSLVLDKLEEHIDHVRFGVVCKNWLSIAKLNHKNHQFRINVPPLLMILSYINYRMLIISQTKKRYLLYSILSEKEYPIQLSHPCIKNMSVCLGFSHGWLALKYDNKAITLVKLFKYSVAPISLPPLENIKKVTLSADPITSPSEYVVAAIYNCGRLALKRASQSFWIRVDINELWCTNVVFYKGLVFADNLGRTIVSFKFNNPPCDDSNDPNFTYYEKIASTRYSVPAQSYGEGRYFVKSLNGDIWMVRRNLIDQYDKSSYNFDVYKLKLDAQSGKLEQMNKLESLEDNILFVGNFGDSISLSASAFSKMEKNSIYFVDESDKDGIELHIYNAEDGSVDSRSLPRPFRKMLHFWVLPQFQWD