AlfalfaGEDB | MS.gene42429

M. sativa cultivar XinJiangDaYe / MS.gene42429

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42429.t1	AES80177.2	74.5	275	62	3	1	268	99	372	1.50E-108	402.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42429.t1	G7L4M9	74.5	275	62	3	1	268	99	372	1.1e-108	402.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene85826	MS.gene42429	PPI
MS.gene99022	MS.gene42429	PPI
MS.gene52164	MS.gene42429	PPI
MS.gene27636	MS.gene42429	PPI
MS.gene42429	MS.gene27639	PPI
MS.gene42429	MS.gene22627	PPI
MS.gene27750	MS.gene42429	PPI
MS.gene52697	MS.gene42429	PPI
MS.gene27747	MS.gene42429	PPI
MS.gene85835	MS.gene42429	PPI
MS.gene031137	MS.gene42429	PPI
MS.gene52261	MS.gene42429	PPI
MS.gene27637	MS.gene42429	PPI
MS.gene42430	MS.gene42429	PPI
MS.gene023888	MS.gene42429	PPI
MS.gene066292	MS.gene42429	PPI
MS.gene73723	MS.gene42429	PPI
MS.gene066289	MS.gene42429	PPI
MS.gene023784	MS.gene42429	PPI
MS.gene76056	MS.gene42429	PPI
MS.gene42226	MS.gene42429	PPI
MS.gene22627	MS.gene42429	PPI
MS.gene42429	MS.gene42432	PPI
MS.gene99024	MS.gene42429	PPI
MS.gene52163	MS.gene42429	PPI
MS.gene42222	MS.gene42429	PPI
MS.gene42431	MS.gene42429	PPI
MS.gene94239	MS.gene42429	PPI
MS.gene023893	MS.gene42429	PPI
MS.gene99023	MS.gene42429	PPI
MS.gene42429	MS.gene52261	PPI
MS.gene42429	MS.gene031137	PPI
MS.gene035137	MS.gene42429	PPI
MS.gene42429	MS.gene031136	PPI
MS.gene52253	MS.gene42429	PPI
MS.gene44542	MS.gene42429	PPI
MS.gene023892	MS.gene42429	PPI
MS.gene42429	MS.gene52164	PPI
MS.gene52262	MS.gene42429	PPI
MS.gene42429	MS.gene023887	PPI
MS.gene27748	MS.gene42429	PPI
MS.gene73726	MS.gene42429	PPI
MS.gene42429	MS.gene76059	PPI
MS.gene52166	MS.gene42429	PPI
MS.gene52259	MS.gene42429	PPI
MS.gene035135	MS.gene42429	PPI
MS.gene42429	MS.gene85835	PPI
MS.gene52255	MS.gene42429	PPI
MS.gene52263	MS.gene42429	PPI
MS.gene94237	MS.gene42429	PPI
MS.gene27753	MS.gene42429	PPI
MS.gene42429	MS.gene27635	PPI
MS.gene49594	MS.gene42429	PPI
MS.gene023891	MS.gene42429	PPI
MS.gene42429	MS.gene52913	PPI
MS.gene42224	MS.gene42429	PPI
MS.gene42429	MS.gene27748	PPI
MS.gene42429	MS.gene42223	PPI
MS.gene42429	MS.gene42428	PPI
MS.gene032784	MS.gene42429	PPI
MS.gene42223	MS.gene42429	PPI
MS.gene29654	MS.gene42429	PPI
MS.gene42429	MS.gene52254	PPI
MS.gene023785	MS.gene42429	PPI
MS.gene42429	MS.gene85826	PPI
MS.gene041287	MS.gene42429	PPI
MS.gene27745	MS.gene42429	PPI
MS.gene42429	MS.gene023890	PPI
MS.gene42433	MS.gene42429	PPI
MS.gene43191	MS.gene42429	PPI
MS.gene027042	MS.gene42429	PPI
MS.gene42429	MS.gene52259	PPI
MS.gene42429	MS.gene97317	PPI
MS.gene73460	MS.gene42429	PPI
MS.gene29651	MS.gene42429	PPI
MS.gene46208	MS.gene42429	PPI
MS.gene42429	MS.gene49592	PPI
MS.gene76061	MS.gene42429	PPI
MS.gene023787	MS.gene42429	PPI
MS.gene86085	MS.gene42429	PPI
MS.gene032785	MS.gene42429	PPI
MS.gene27751	MS.gene42429	PPI
MS.gene42429	MS.gene27637	PPI
MS.gene52254	MS.gene42429	PPI
MS.gene42429	MS.gene95450	PPI
MS.gene49593	MS.gene42429	PPI
MS.gene032780	MS.gene42429	PPI
MS.gene066291	MS.gene42429	PPI
MS.gene023889	MS.gene42429	PPI
MS.gene029514	MS.gene42429	PPI
MS.gene022086	MS.gene42429	PPI
MS.gene27754	MS.gene42429	PPI
MS.gene023887	MS.gene42429	PPI
MS.gene27749	MS.gene42429	PPI
MS.gene52258	MS.gene42429	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42429.t1	MTR_7g077650	74.545	275	62	3	1	268	99	372	8.49e-141	400
MS.gene42429.t1	MTR_7g077660	74.359	273	61	5	2	268	99	368	6.57e-138	393
MS.gene42429.t1	MTR_7g077620	65.809	272	84	4	2	266	101	370	7.36e-123	355
MS.gene42429.t1	MTR_7g076110	54.839	279	111	6	4	268	36	313	3.97e-97	287
MS.gene42429.t1	MTR_7g076100	58.824	238	87	5	39	268	66	300	1.48e-87	263
MS.gene42429.t1	MTR_1g108740	46.442	267	118	12	6	266	97	344	3.24e-58	189
MS.gene42429.t1	MTR_5g038260	42.244	303	128	11	2	268	92	383	8.87e-55	181
MS.gene42429.t1	MTR_5g038230	42.336	274	93	10	2	244	90	329	9.72e-50	167
MS.gene42429.t1	MTR_1g064570	37.846	325	129	17	2	268	91	400	1.36e-43	152
MS.gene42429.t1	MTR_5g082770	44.651	215	90	7	4	204	88	287	3.63e-43	149
MS.gene42429.t1	MTR_7g076130	56.911	123	49	2	71	190	27	148	9.25e-40	135
MS.gene42429.t1	MTR_5g082780	38.889	234	105	11	15	232	1	212	2.58e-34	124
MS.gene42429.t1	MTR_1g016150	50.575	87	39	3	6	89	106	191	1.12e-18	82.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 49 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTTATATTGTTAATGATTT+AGG	0.039594	7.2:-28430021	MS.gene42429:CDS
AAAGACCAAGCCAATCATTT+TGG	0.213196	7.2:-28430482	MS.gene42429:CDS
TTTATATTGTTAATGATTTA+GGG	0.247410	7.2:-28430020	MS.gene42429:CDS
CCATGATAACGACGTAGTTT+TGG	0.306817	7.2:+28430279	None:intergenic
AATGACCAAACTTGAGAGTT+TGG	0.311921	7.2:-28430127	MS.gene42429:CDS
AATTACAACTTTCCTACAAC+TGG	0.317321	7.2:-28429954	MS.gene42429:CDS
GTCTTCCAAACTCTCAAGTT+TGG	0.322441	7.2:+28430122	None:intergenic
GTTGCAGCAATTTACAAGTT+TGG	0.322860	7.2:-28430519	MS.gene42429:CDS
GGAGGTACACTATACTTAAA+AGG	0.322941	7.2:+28430621	None:intergenic
TTGTAGGAAAGTTGTAATTT+TGG	0.337038	7.2:+28429958	None:intergenic
CCATGGTTGGCTTGCATTAG+TGG	0.344716	7.2:-28430673	MS.gene42429:CDS
GGGTTCTACCACAACTTCAT+TGG	0.363398	7.2:-28429918	MS.gene42429:CDS
TTCGAGAGGTGGCAGACTAA+TGG	0.384467	7.2:+28430599	None:intergenic
AGCCTCTTGTTTCTCCAAGT+TGG	0.414433	7.2:-28430055	MS.gene42429:CDS
AAAACGTTGCATCCAGTTGT+AGG	0.414803	7.2:+28429942	None:intergenic
AATCATTGACCGGAGAAATA+TGG	0.417114	7.2:-28430239	MS.gene42429:CDS
TTGTTTCTCCAAGTTGGAAA+AGG	0.437802	7.2:-28430049	MS.gene42429:CDS
TCGAGAGGTGGCAGACTAAT+GGG	0.438780	7.2:+28430600	None:intergenic
ACTAGCCATCTTCTCATAGT+AGG	0.443005	7.2:+28430317	None:intergenic
GGTTCTACCACAACTTCATT+GGG	0.443765	7.2:-28429917	MS.gene42429:CDS
AGGAGGATTGTTTAGCTTGA+AGG	0.455047	7.2:+28430368	None:intergenic
GTTTGGGCTGTAGCCATGGT+TGG	0.500900	7.2:-28430686	MS.gene42429:CDS
CTACGCGAATCCAAAATGAT+TGG	0.502017	7.2:+28430472	None:intergenic
TAATCACTTGGATTTGTCAC+GGG	0.505018	7.2:+28430546	None:intergenic
CCAAAACTACGTCGTTATCA+TGG	0.509352	7.2:-28430279	MS.gene42429:CDS
TCTCATCAACCATATTTCTC+CGG	0.510997	7.2:+28430230	None:intergenic
CGAATCCAAAATGATTGGCT+TGG	0.520142	7.2:+28430477	None:intergenic
CCAGTTCCAATTTGAAAACG+TGG	0.529346	7.2:+28430175	None:intergenic
CAGTTCCAATTTGAAAACGT+GGG	0.532083	7.2:+28430176	None:intergenic
ACCTCTCGAATCTGTTTCCA+AGG	0.534304	7.2:-28430586	MS.gene42429:CDS
GAGATCTTTAACGTCAAGGA+TGG	0.538671	7.2:-28429994	MS.gene42429:CDS
GTCAGCAGACAAGGTAACCT+TGG	0.544724	7.2:+28430569	None:intergenic
GTTAGAGATCTTTAACGTCA+AGG	0.568886	7.2:-28429998	MS.gene42429:CDS
TGTCACGGGGTCAGCAGACA+AGG	0.572198	7.2:+28430560	None:intergenic
TTGGAACTGGATGCCGAGAG+TGG	0.572302	7.2:-28430162	MS.gene42429:CDS
GGATCATTTGAGTCATCAGA+AGG	0.579857	7.2:+28430348	None:intergenic
ATAATCACTTGGATTTGTCA+CGG	0.582648	7.2:+28430545	None:intergenic
GTTTAGCTTGAAGGATACAA+TGG	0.589118	7.2:+28430377	None:intergenic
GCTGCAACCACATAATCACT+TGG	0.591162	7.2:+28430534	None:intergenic
TTCCAACTTGGAGAAACAAG+AGG	0.597328	7.2:+28430053	None:intergenic
GACAAATCCAAGTGATTATG+TGG	0.609021	7.2:-28430541	MS.gene42429:CDS
GCTCAATCCCAATGAAGTTG+TGG	0.625686	7.2:+28429910	None:intergenic
CCACTAATGCAAGCCAACCA+TGG	0.628857	7.2:+28430673	None:intergenic
TCATTTGAGTCATCAGAAGG+AGG	0.630870	7.2:+28430351	None:intergenic
TTGGAAACAGATTCGAGAGG+TGG	0.643460	7.2:+28430588	None:intergenic
AGTTCCAATTTGAAAACGTG+GGG	0.649411	7.2:+28430177	None:intergenic
AATCACTTGGATTTGTCACG+GGG	0.664381	7.2:+28430547	None:intergenic
AGAGGTGGCAGACTAATGGG+AGG	0.733529	7.2:+28430603	None:intergenic
ACCTTGGAAACAGATTCGAG+AGG	0.760909	7.2:+28430585	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTATATTGTTAATGATTT+AGG	-	chr7.2:28430587-28430606	MS.gene42429:CDS	10.0%
!!	TTTATATTGTTAATGATTTA+GGG	-	chr7.2:28430588-28430607	MS.gene42429:CDS	10.0%
!!!	ACGAATATTGTGTTTTATAA+AGG	-	chr7.2:28430179-28430198	MS.gene42429:CDS	20.0%
!	TTCTCATAGTAGGAAAAATT+AGG	+	chr7.2:28430284-28430303	None:intergenic	25.0%
!!	TATTGTGTTTTATAAAGGCA+TGG	-	chr7.2:28430184-28430203	MS.gene42429:CDS	25.0%
!!	TATTTTGTGAAATCATTGAC+CGG	-	chr7.2:28430359-28430378	MS.gene42429:CDS	25.0%
!!	TTGTAGGAAAGTTGTAATTT+TGG	+	chr7.2:28430653-28430672	None:intergenic	25.0%
!!!	ATTTTATTTGTCAGAAGTGT+TGG	-	chr7.2:28430512-28430531	MS.gene42429:CDS	25.0%
	AATTACAACTTTCCTACAAC+TGG	-	chr7.2:28430654-28430673	MS.gene42429:CDS	30.0%
	ATAATCACTTGGATTTGTCA+CGG	+	chr7.2:28430066-28430085	None:intergenic	30.0%
!	AATTGAATCCTTTTCCAACT+TGG	+	chr7.2:28430570-28430589	None:intergenic	30.0%
!	TTTTTCCTACTATGAGAAGA+TGG	-	chr7.2:28430286-28430305	MS.gene42429:CDS	30.0%
!!!	CGTAGTTTTGGAATCATATA+TGG	+	chr7.2:28430320-28430339	None:intergenic	30.0%
	AATCATTGACCGGAGAAATA+TGG	-	chr7.2:28430369-28430388	MS.gene42429:CDS	35.0%
	AATGACCAAACTTGAGAGTT+TGG	-	chr7.2:28430481-28430500	MS.gene42429:CDS	35.0%
	AGTTCCAATTTGAAAACGTG+GGG	+	chr7.2:28430434-28430453	None:intergenic	35.0%
	CAGTTCCAATTTGAAAACGT+GGG	+	chr7.2:28430435-28430454	None:intergenic	35.0%
	GACAAATCCAAGTGATTATG+TGG	-	chr7.2:28430067-28430086	MS.gene42429:CDS	35.0%
	GTTAGAGATCTTTAACGTCA+AGG	-	chr7.2:28430610-28430629	MS.gene42429:CDS	35.0%
	GTTGCAGCAATTTACAAGTT+TGG	-	chr7.2:28430089-28430108	MS.gene42429:CDS	35.0%
	TAATCACTTGGATTTGTCAC+GGG	+	chr7.2:28430065-28430084	None:intergenic	35.0%
	TCTCATCAACCATATTTCTC+CGG	+	chr7.2:28430381-28430400	None:intergenic	35.0%
!	AAAGACCAAGCCAATCATTT+TGG	-	chr7.2:28430126-28430145	MS.gene42429:CDS	35.0%
!	GGAGGTACACTATACTTAAA+AGG	+	chr7.2:28429990-28430009	None:intergenic	35.0%
!	TTGTTTCTCCAAGTTGGAAA+AGG	-	chr7.2:28430559-28430578	MS.gene42429:CDS	35.0%
!!	GTTTAGCTTGAAGGATACAA+TGG	+	chr7.2:28430234-28430253	None:intergenic	35.0%
!!	TTGTTCAAGTTTTCCACTCT+CGG	+	chr7.2:28430462-28430481	None:intergenic	35.0%
	AAAACGTTGCATCCAGTTGT+AGG	+	chr7.2:28430669-28430688	None:intergenic	40.0%
	AATCACTTGGATTTGTCACG+GGG	+	chr7.2:28430064-28430083	None:intergenic	40.0%
	ACTAGCCATCTTCTCATAGT+AGG	+	chr7.2:28430294-28430313	None:intergenic	40.0%
	CCAAAACTACGTCGTTATCA+TGG	-	chr7.2:28430329-28430348	MS.gene42429:CDS	40.0%
	CCAGTTCCAATTTGAAAACG+TGG	+	chr7.2:28430436-28430455	None:intergenic	40.0%
	CTACGCGAATCCAAAATGAT+TGG	+	chr7.2:28430139-28430158	None:intergenic	40.0%
	GAGATCTTTAACGTCAAGGA+TGG	-	chr7.2:28430614-28430633	MS.gene42429:CDS	40.0%
	GGATCATTTGAGTCATCAGA+AGG	+	chr7.2:28430263-28430282	None:intergenic	40.0%
	GGTTCTACCACAACTTCATT+GGG	-	chr7.2:28430691-28430710	MS.gene42429:CDS	40.0%
	GTCTTCCAAACTCTCAAGTT+TGG	+	chr7.2:28430489-28430508	None:intergenic	40.0%
	TCATTTGAGTCATCAGAAGG+AGG	+	chr7.2:28430260-28430279	None:intergenic	40.0%
	TTCCAACTTGGAGAAACAAG+AGG	+	chr7.2:28430558-28430577	None:intergenic	40.0%
!	ACAACTGGATGCAACGTTTT+TGG	-	chr7.2:28430669-28430688	MS.gene42429:CDS	40.0%
!	CAAACCCCACGTTTTCAAAT+TGG	-	chr7.2:28430427-28430446	MS.gene42429:CDS	40.0%
!	CAACTGGATGCAACGTTTTT+GGG	-	chr7.2:28430670-28430689	MS.gene42429:CDS	40.0%
!	CCACGTTTTCAAATTGGAAC+TGG	-	chr7.2:28430433-28430452	MS.gene42429:CDS	40.0%
!	CGAATCCAAAATGATTGGCT+TGG	+	chr7.2:28430134-28430153	None:intergenic	40.0%
!!	AGGAGGATTGTTTAGCTTGA+AGG	+	chr7.2:28430243-28430262	None:intergenic	40.0%
!!	CCATGATAACGACGTAGTTT+TGG	+	chr7.2:28430332-28430351	None:intergenic	40.0%
	ACCTCTCGAATCTGTTTCCA+AGG	-	chr7.2:28430022-28430041	MS.gene42429:CDS	45.0%
	ACCTTGGAAACAGATTCGAG+AGG	+	chr7.2:28430026-28430045	None:intergenic	45.0%
	GCTGCAACCACATAATCACT+TGG	+	chr7.2:28430077-28430096	None:intergenic	45.0%
	GGGTTCTACCACAACTTCAT+TGG	-	chr7.2:28430690-28430709	MS.gene42429:CDS	45.0%
	TTGGAAACAGATTCGAGAGG+TGG	+	chr7.2:28430023-28430042	None:intergenic	45.0%
!	AGCCTCTTGTTTCTCCAAGT+TGG	-	chr7.2:28430553-28430572	MS.gene42429:CDS	45.0%
!!	TTTTGTTTGGGCTGTAGCCA+TGG	-	chr7.2:28429918-28429937	MS.gene42429:CDS	45.0%
	CCACTAATGCAAGCCAACCA+TGG	+	chr7.2:28429938-28429957	None:intergenic	50.0%
	GTCAGCAGACAAGGTAACCT+TGG	+	chr7.2:28430042-28430061	None:intergenic	50.0%
	TCGAGAGGTGGCAGACTAAT+GGG	+	chr7.2:28430011-28430030	None:intergenic	50.0%
	TTCGAGAGGTGGCAGACTAA+TGG	+	chr7.2:28430012-28430031	None:intergenic	50.0%
!!	CCATGGTTGGCTTGCATTAG+TGG	-	chr7.2:28429935-28429954	MS.gene42429:CDS	50.0%
	AGAGGTGGCAGACTAATGGG+AGG	+	chr7.2:28430008-28430027	None:intergenic	55.0%
	GTTTGGGCTGTAGCCATGGT+TGG	-	chr7.2:28429922-28429941	MS.gene42429:CDS	55.0%
	TTGGAACTGGATGCCGAGAG+TGG	-	chr7.2:28430446-28430465	MS.gene42429:CDS	55.0%
	TGTCACGGGGTCAGCAGACA+AGG	+	chr7.2:28430051-28430070	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	28429912	28430718	28429912	ID=MS.gene42429
chr7.2	mRNA	28429912	28430718	28429912	ID=MS.gene42429.t1;Parent=MS.gene42429
chr7.2	exon	28429912	28430718	28429912	ID=MS.gene42429.t1.exon1;Parent=MS.gene42429.t1
chr7.2	CDS	28429912	28430718	28429912	ID=cds.MS.gene42429.t1;Parent=MS.gene42429.t1

Gene Sequence

>MS.gene42429

ATGAAATTTTGTTTGGGCTGTAGCCATGGTTGGCTTGCATTAGTGGATGATAACGATGTCATTACTCTAGTGAATCCTTTTAAGTATAGTGTACCTCCCATTAGTCTGCCACCTCTCGAATCTGTTTCCAAGGTTACCTTGTCTGCTGACCCCGTGACAAATCCAAGTGATTATGTGGTTGCAGCAATTTACAAGTTTGGTTATCTCGCTTTCAAAAGACCAAGCCAATCATTTTGGATTCGCGTAGACACAAATGAATTTACTTTCACGAATATTGTGTTTTATAAAGGCATGGTATTTGCTGATAGCTGCAGAGATACCATTGTATCCTTCAAGCTAAACAATCCTCCTTCTGATGACTCAAATGATCCTAATTTTTCCTACTATGAGAAGATGGCTAGTACTCCATATATGATTCCAAAACTACGTCGTTATCATGGACTTGCTTATTTTGTGAAATCATTGACCGGAGAAATATGGTTGATGAGAAGATGCTTAATCGACGATTTTTCACTCAAACCCCACGTTTTCAAATTGGAACTGGATGCCGAGAGTGGAAAACTTGAACAAATGACCAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTCAGAAGTGTTGGTGATTCAATTTCTGTGTCAGCCTCTTGTTTCTCCAAGTTGGAAAAGGATTCAATTTATATTGTTAATGATTTAGGGTTAGAGATCTTTAACGTCAAGGATGGAAGCTTTCAGAGACCAAAATTACAACTTTCCTACAACTGGATGCAACGTTTTTGGGTTCTACCACAACTTCATTGGGATTGA

Protein sequence

>MS.gene42429.t1

MKFCLGCSHGWLALVDDNDVITLVNPFKYSVPPISLPPLESVSKVTLSADPVTNPSDYVVAAIYKFGYLAFKRPSQSFWIRVDTNEFTFTNIVFYKGMVFADSCRDTIVSFKLNNPPSDDSNDPNFSYYEKMASTPYMIPKLRRYHGLAYFVKSLTGEIWLMRRCLIDDFSLKPHVFKLELDAESGKLEQMTKLESLEDNILFVRSVGDSISVSASCFSKLEKDSIYIVNDLGLEIFNVKDGSFQRPKLQLSYNWMQRFWVLPQLHWD