AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27745

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27745.t1 MTR_7g077620 72.386 373 95 5 1 368 1 370 0.0 527
MS.gene27745.t1 MTR_7g077660 69.705 373 105 6 1 370 1 368 3.96e-174 489
MS.gene27745.t1 MTR_7g077650 68.325 382 99 7 1 370 1 372 2.70e-172 484
MS.gene27745.t1 MTR_7g076110 59.060 298 108 7 82 370 21 313 1.42e-109 323
MS.gene27745.t1 MTR_7g076100 65.272 239 76 5 135 370 66 300 3.73e-95 286
MS.gene27745.t1 MTR_7g076100 79.630 54 10 1 1 53 1 54 2.48e-21 93.2
MS.gene27745.t1 MTR_5g038260 43.358 399 176 14 3 370 4 383 4.40e-89 273
MS.gene27745.t1 MTR_1g108740 46.381 373 166 14 1 368 1 344 5.32e-88 269
MS.gene27745.t1 MTR_5g038230 41.772 395 154 14 5 368 4 353 1.07e-80 251
MS.gene27745.t1 MTR_5g082770 47.097 310 127 9 5 302 4 288 1.35e-74 233
MS.gene27745.t1 MTR_1g064570 41.007 417 179 19 3 370 2 400 2.30e-73 233
MS.gene27745.t1 MTR_1g016150 52.486 181 78 6 1 175 1 179 3.72e-51 171
MS.gene27745.t1 MTR_7g076130 64.286 126 40 3 165 287 25 148 2.09e-45 153
MS.gene27745.t1 MTR_5g082780 40.789 228 104 8 111 326 1 209 7.41e-38 136
MS.gene27745.t1 MTR_1g108750 49.673 153 44 5 5 156 4 124 9.15e-27 103
MS.gene27745.t1 MTR_3g114970 24.734 376 206 16 4 347 3 333 2.20e-16 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGGTGATAAGAAGTATTT+AGG 0.155147 7.3:-29219072 MS.gene27745:CDS
CAAACTCCATGTTTACAAAT+TGG 0.204599 7.3:-29219247 MS.gene27745:CDS
AAAGACCAAGCCAATCATTT+TGG 0.213606 7.3:-29219551 MS.gene27745:CDS
TTCTCATAGTAAGTAAAATT+AGG 0.229231 7.3:+29219393 None:intergenic
TGAAAGCGAGAAAACTAAAA+AGG 0.276479 7.3:+29219574 None:intergenic
AATAAACAAACTTGAGAGTT+TGG 0.296170 7.3:-29219193 MS.gene27745:CDS
GGCGGTACACTATACTTAAA+AGG 0.310810 7.3:+29219690 None:intergenic
TAATCACTTGGGCTTGTAAT+GGG 0.338599 7.3:+29219615 None:intergenic
AAAAGGATTCACTAGTGTTA+TGG 0.340587 7.3:+29219707 None:intergenic
TGATGATCCTCTCACATAAT+TGG 0.345632 7.3:-29219881 MS.gene27745:CDS
GAAGGATGGGATATCTGAAT+AGG 0.346154 7.3:+29219798 None:intergenic
AAGAACACATAGATCATGTT+AGG 0.348741 7.3:-29219989 MS.gene27745:CDS
AGAAGCATATCCTCTTAGTT+TGG 0.367450 7.3:-29220027 MS.gene27745:CDS
CATTGAAAGTAAAACGTGTA+TGG 0.370132 7.3:-29219772 MS.gene27745:CDS
CTCCAAGTTGGAAAAGGATT+CGG 0.375437 7.3:-29219109 MS.gene27745:CDS
TTCGAGAGGTGGCAGACTAA+TGG 0.384467 7.3:+29219668 None:intergenic
TCTCATAGTAAGTAAAATTA+GGG 0.387493 7.3:+29219394 None:intergenic
CACATAGATCATGTTAGGTT+TGG 0.400301 7.3:-29219984 MS.gene27745:CDS
CCATGTTTACAAATTGGAAC+TGG 0.402360 7.3:-29219241 MS.gene27745:CDS
ATATCCTCTTAGTTTGGTTC+TGG 0.403102 7.3:-29220021 MS.gene27745:CDS
AGCCTCTTGTTTCTCCAAGT+TGG 0.404761 7.3:-29219121 MS.gene27745:CDS
GGGTTCTACCACAATTTCAA+TGG 0.408660 7.3:-29218969 MS.gene27745:CDS
TCATGGTTGGCTTGCATTGG+TGG 0.434138 7.3:-29219742 MS.gene27745:CDS
AAGGTGATAAGAAGTATTTA+GGG 0.434866 7.3:-29219071 MS.gene27745:CDS
TTGTTTCTCCAAGTTGGAAA+AGG 0.437802 7.3:-29219115 MS.gene27745:CDS
TCGAGAGGTGGCAGACTAAT+GGG 0.438780 7.3:+29219669 None:intergenic
CTACATGACTCCAAAATGAT+TGG 0.451480 7.3:+29219541 None:intergenic
TACTTTCAATGATGGAAGGA+TGG 0.467120 7.3:+29219784 None:intergenic
TAGCAATGAGGAAGGTAGAC+TGG 0.468564 7.3:-29220058 None:intergenic
ATGTGAAGGATGGAAGCTTT+CGG 0.471634 7.3:-29219035 MS.gene27745:CDS
TTGGCGTGGTGTGCAAGAAT+TGG 0.475029 7.3:-29219965 MS.gene27745:CDS
ATAATCACTTGGGCTTGTAA+TGG 0.476686 7.3:+29219614 None:intergenic
GGTTCTACCACAATTTCAAT+GGG 0.480142 7.3:-29218968 MS.gene27745:CDS
TAGTTTGGTTCTGGATAAGT+TGG 0.481337 7.3:-29220012 MS.gene27745:CDS
TAGTCATGGTTGGCTTGCAT+TGG 0.486863 7.3:-29219745 MS.gene27745:CDS
AAGGATGGGATATCTGAATA+GGG 0.489334 7.3:+29219799 None:intergenic
AGGAGGATTGTTAAGCTTGA+AGG 0.497543 7.3:+29219434 None:intergenic
TACAAGCCCAAGTGATTATG+TGG 0.499442 7.3:-29219610 MS.gene27745:CDS
ACTTTCAATGATGGAAGGAT+GGG 0.503431 7.3:+29219785 None:intergenic
TATTTCGTGAAATCATTGAC+TGG 0.505540 7.3:-29219315 MS.gene27745:CDS
TTATCCAGAACCAAACTAAG+AGG 0.508795 7.3:+29220017 None:intergenic
GAGATCTATAATGTGAAGGA+TGG 0.509549 7.3:-29219045 MS.gene27745:CDS
CCAGTTCCAATTTGTAAACA+TGG 0.513473 7.3:+29219241 None:intergenic
CGGCTATAATAAGATTGCTC+TGG 0.516174 7.3:+29219345 None:intergenic
ATTGAAAGTAAAACGTGTAT+GGG 0.523237 7.3:-29219771 MS.gene27745:CDS
AATCATTGACTGGAGACATA+TGG 0.524535 7.3:-29219305 MS.gene27745:CDS
GCTGCAACCACATAATCACT+TGG 0.531804 7.3:+29219603 None:intergenic
TTGGAACTGGATGTCCAGAA+TGG 0.546513 7.3:-29219228 MS.gene27745:CDS
CTCTTTCTTAGCAATGAGGA+AGG 0.560180 7.3:-29220066 None:intergenic
TGCATTCGCTCGAAGGAAAG+TGG 0.562548 7.3:+29219000 None:intergenic
TTGGTATTTGCCAACAGTGA+CGG 0.565522 7.3:-29219471 MS.gene27745:CDS
TGTAATGGGGTCAGCAGACA+AGG 0.568289 7.3:+29219629 None:intergenic
TGACTCCAAAATGATTGGCT+TGG 0.571542 7.3:+29219546 None:intergenic
GGTCAATCCCATTGAAATTG+TGG 0.574786 7.3:+29218961 None:intergenic
TTGGTCTAATATATCAAAGA+CGG 0.581444 7.3:-29219862 MS.gene27745:CDS
GTATGGGCTGTAGTCATGGT+TGG 0.586884 7.3:-29219755 MS.gene27745:CDS
CTGCAACCACATAATCACTT+GGG 0.596035 7.3:+29219604 None:intergenic
TTCCAACTTGGAGAAACAAG+AGG 0.597328 7.3:+29219119 None:intergenic
GTTAGAGATCTATAATGTGA+AGG 0.603732 7.3:-29219049 MS.gene27745:CDS
TTGTTGAGAGATTCGAGAGG+TGG 0.604059 7.3:+29219657 None:intergenic
AATCACTTGGGCTTGTAATG+GGG 0.606028 7.3:+29219616 None:intergenic
AACTTGGAGAAACAAGAGGC+TGG 0.606698 7.3:+29219123 None:intergenic
AATAGTGTCTCCGTCACTGT+TGG 0.610668 7.3:+29219461 None:intergenic
AGCAATGAGGAAGGTAGACT+GGG 0.614849 7.3:-29220057 None:intergenic
ACGTGTATGGGCTGTAGTCA+TGG 0.619342 7.3:-29219759 MS.gene27745:CDS
TATTAGACCAATTATGTGAG+AGG 0.620183 7.3:+29219874 None:intergenic
TGTGAGAGGATCATCAACAT+AGG 0.620354 7.3:+29219888 None:intergenic
AGATCATGTTAGGTTTGGCG+TGG 0.628602 7.3:-29219979 MS.gene27745:CDS
GGGTCATTTGAGTCATCACA+AGG 0.644746 7.3:+29219414 None:intergenic
ACCTCTCGAATCTCTCAACA+AGG 0.651432 7.3:-29219655 MS.gene27745:CDS
ATGATTTCACGAAATAAGCA+CGG 0.674470 7.3:+29219322 None:intergenic
GAGAGGATCATCAACATAGG+TGG 0.682169 7.3:+29219891 None:intergenic
ATTGACTGGAGACATATGGA+TGG 0.700403 7.3:-29219301 MS.gene27745:CDS
TCATTTGAGTCATCACAAGG+AGG 0.722989 7.3:+29219417 None:intergenic
AGAGGTGGCAGACTAATGGG+CGG 0.746430 7.3:+29219672 None:intergenic
ACCTTGTTGAGAGATTCGAG+AGG 0.768126 7.3:+29219654 None:intergenic
ATTTCACGAAATAAGCACGG+CGG 0.813610 7.3:+29219325 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCATAAAAATCATCAATTT+AGG - chr7.3:29219093-29219112 MS.gene27745:CDS 20.0%
!! TCTCATAGTAAGTAAAATTA+GGG + chr7.3:29219625-29219644 None:intergenic 20.0%
!! TTCTCATAGTAAGTAAAATT+AGG + chr7.3:29219626-29219645 None:intergenic 20.0%
!!! ACTGATGTTGTATTTTATAA+AGG - chr7.3:29219521-29219540 MS.gene27745:CDS 20.0%
!!! TCCTAAATTGATGATTTTTA+TGG + chr7.3:29219097-29219116 None:intergenic 20.0%
! AATAAACAAACTTGAGAGTT+TGG - chr7.3:29219823-29219842 MS.gene27745:CDS 25.0%
! ATTGAAAGTAAAACGTGTAT+GGG - chr7.3:29219245-29219264 MS.gene27745:CDS 25.0%
! TTGGTCTAATATATCAAAGA+CGG - chr7.3:29219154-29219173 MS.gene27745:CDS 25.0%
!! TGTTGTATTTTATAAAGGCT+TGG - chr7.3:29219526-29219545 MS.gene27745:CDS 25.0%
!!! AAGGTGATAAGAAGTATTTA+GGG - chr7.3:29219945-29219964 MS.gene27745:CDS 25.0%
!!! GAAGACAACATTTTATTTGT+CGG - chr7.3:29219845-29219864 MS.gene27745:CDS 25.0%
AAAAGGATTCACTAGTGTTA+TGG + chr7.3:29219312-29219331 None:intergenic 30.0%
AAGAACACATAGATCATGTT+AGG - chr7.3:29219027-29219046 MS.gene27745:CDS 30.0%
ATGATTTCACGAAATAAGCA+CGG + chr7.3:29219697-29219716 None:intergenic 30.0%
CAAACTCCATGTTTACAAAT+TGG - chr7.3:29219769-29219788 MS.gene27745:CDS 30.0%
CATTGAAAGTAAAACGTGTA+TGG - chr7.3:29219244-29219263 MS.gene27745:CDS 30.0%
GTTAGAGATCTATAATGTGA+AGG - chr7.3:29219967-29219986 MS.gene27745:CDS 30.0%
TATTAGACCAATTATGTGAG+AGG + chr7.3:29219145-29219164 None:intergenic 30.0%
TATTTCGTGAAATCATTGAC+TGG - chr7.3:29219701-29219720 MS.gene27745:CDS 30.0%
TGAAAGCGAGAAAACTAAAA+AGG + chr7.3:29219445-29219464 None:intergenic 30.0%
! ACACGTTTTACTTTCAATGA+TGG + chr7.3:29219243-29219262 None:intergenic 30.0%
!! ATTTTATTTGTCGGAATCTG+TGG - chr7.3:29219854-29219873 MS.gene27745:CDS 30.0%
!! GAAGGTGATAAGAAGTATTT+AGG - chr7.3:29219944-29219963 MS.gene27745:CDS 30.0%
!! GTTTTACTTTCAATGATGGA+AGG + chr7.3:29219239-29219258 None:intergenic 30.0%
!! TTTGTTCAAGTTTTCCATTC+TGG + chr7.3:29219805-29219824 None:intergenic 30.0%
!!! TCGGTTTATTTTGTTTGTGA+AGG - chr7.3:29219926-29219945 MS.gene27745:CDS 30.0%
AAGGATGGGATATCTGAATA+GGG + chr7.3:29219220-29219239 None:intergenic 35.0%
ATAATCACTTGGGCTTGTAA+TGG + chr7.3:29219405-29219424 None:intergenic 35.0%
CACATAGATCATGTTAGGTT+TGG - chr7.3:29219032-29219051 MS.gene27745:CDS 35.0%
CCAGTTCCAATTTGTAAACA+TGG + chr7.3:29219778-29219797 None:intergenic 35.0%
CCATGTTTACAAATTGGAAC+TGG - chr7.3:29219775-29219794 MS.gene27745:CDS 35.0%
CTACATGACTCCAAAATGAT+TGG + chr7.3:29219478-29219497 None:intergenic 35.0%
GAGATCTATAATGTGAAGGA+TGG - chr7.3:29219971-29219990 MS.gene27745:CDS 35.0%
GGTTCTACCACAATTTCAAT+GGG - chr7.3:29220048-29220067 MS.gene27745:CDS 35.0%
TAATCACTTGGGCTTGTAAT+GGG + chr7.3:29219404-29219423 None:intergenic 35.0%
TGATGATCCTCTCACATAAT+TGG - chr7.3:29219135-29219154 MS.gene27745:CDS 35.0%
TTATCCAGAACCAAACTAAG+AGG + chr7.3:29219002-29219021 None:intergenic 35.0%
! AAAGACCAAGCCAATCATTT+TGG - chr7.3:29219465-29219484 MS.gene27745:CDS 35.0%
! AATCATTGACTGGAGACATA+TGG - chr7.3:29219711-29219730 MS.gene27745:CDS 35.0%
! ACTTTCAATGATGGAAGGAT+GGG + chr7.3:29219234-29219253 None:intergenic 35.0%
! AGAAGCATATCCTCTTAGTT+TGG - chr7.3:29218989-29219008 MS.gene27745:CDS 35.0%
! ATATCCTCTTAGTTTGGTTC+TGG - chr7.3:29218995-29219014 MS.gene27745:CDS 35.0%
! TACTTTCAATGATGGAAGGA+TGG + chr7.3:29219235-29219254 None:intergenic 35.0%
! TTGTTTCTCCAAGTTGGAAA+AGG - chr7.3:29219901-29219920 MS.gene27745:CDS 35.0%
!! TAGTTTGGTTCTGGATAAGT+TGG - chr7.3:29219004-29219023 MS.gene27745:CDS 35.0%
AAAAAGTTGCATTCGCTCGA+AGG + chr7.3:29220026-29220045 None:intergenic 40.0%
AATCACTTGGGCTTGTAATG+GGG + chr7.3:29219403-29219422 None:intergenic 40.0%
AGGAGGATTGTTAAGCTTGA+AGG + chr7.3:29219585-29219604 None:intergenic 40.0%
ATGTGAAGGATGGAAGCTTT+CGG - chr7.3:29219981-29220000 MS.gene27745:CDS 40.0%
ATTGACTGGAGACATATGGA+TGG - chr7.3:29219715-29219734 MS.gene27745:CDS 40.0%
ATTTCACGAAATAAGCACGG+CGG + chr7.3:29219694-29219713 None:intergenic 40.0%
CGGCTATAATAAGATTGCTC+TGG + chr7.3:29219674-29219693 None:intergenic 40.0%
CTGCAACCACATAATCACTT+GGG + chr7.3:29219415-29219434 None:intergenic 40.0%
GAAGGATGGGATATCTGAAT+AGG + chr7.3:29219221-29219240 None:intergenic 40.0%
GGCGGTACACTATACTTAAA+AGG + chr7.3:29219329-29219348 None:intergenic 40.0%
GGGTTCTACCACAATTTCAA+TGG - chr7.3:29220047-29220066 MS.gene27745:CDS 40.0%
TACAAGCCCAAGTGATTATG+TGG - chr7.3:29219406-29219425 MS.gene27745:CDS 40.0%
TCATTTGAGTCATCACAAGG+AGG + chr7.3:29219602-29219621 None:intergenic 40.0%
TGTGAGAGGATCATCAACAT+AGG + chr7.3:29219131-29219150 None:intergenic 40.0%
TTCCAACTTGGAGAAACAAG+AGG + chr7.3:29219900-29219919 None:intergenic 40.0%
TTGGTATTTGCCAACAGTGA+CGG - chr7.3:29219545-29219564 MS.gene27745:CDS 40.0%
! AACCGAATCCTTTTCCAACT+TGG + chr7.3:29219912-29219931 None:intergenic 40.0%
! CTCCAAGTTGGAAAAGGATT+CGG - chr7.3:29219907-29219926 MS.gene27745:CDS 40.0%
! TGACTCCAAAATGATTGGCT+TGG + chr7.3:29219473-29219492 None:intergenic 40.0%
!!! CGAGCGAATGCAACTTTTTT+GGG - chr7.3:29220027-29220046 MS.gene27745:CDS 40.0%
!!! TCGAGCGAATGCAACTTTTT+TGG - chr7.3:29220026-29220045 MS.gene27745:CDS 40.0%
AACTTGGAGAAACAAGAGGC+TGG + chr7.3:29219896-29219915 None:intergenic 45.0%
AATAGTGTCTCCGTCACTGT+TGG + chr7.3:29219558-29219577 None:intergenic 45.0%
ACCTCTCGAATCTCTCAACA+AGG - chr7.3:29219361-29219380 MS.gene27745:CDS 45.0%
ACCTTGTTGAGAGATTCGAG+AGG + chr7.3:29219365-29219384 None:intergenic 45.0%
AGATCATGTTAGGTTTGGCG+TGG - chr7.3:29219037-29219056 MS.gene27745:CDS 45.0%
GAGAGGATCATCAACATAGG+TGG + chr7.3:29219128-29219147 None:intergenic 45.0%
GCTGCAACCACATAATCACT+TGG + chr7.3:29219416-29219435 None:intergenic 45.0%
GGGTCATTTGAGTCATCACA+AGG + chr7.3:29219605-29219624 None:intergenic 45.0%
TTGGAACTGGATGTCCAGAA+TGG - chr7.3:29219788-29219807 MS.gene27745:CDS 45.0%
TTGTTGAGAGATTCGAGAGG+TGG + chr7.3:29219362-29219381 None:intergenic 45.0%
! AGCCTCTTGTTTCTCCAAGT+TGG - chr7.3:29219895-29219914 MS.gene27745:CDS 45.0%
!! TAGTCATGGTTGGCTTGCAT+TGG - chr7.3:29219271-29219290 MS.gene27745:CDS 45.0%
TCGAGAGGTGGCAGACTAAT+GGG + chr7.3:29219350-29219369 None:intergenic 50.0%
TGCATTCGCTCGAAGGAAAG+TGG + chr7.3:29220019-29220038 None:intergenic 50.0%
TTCGAGAGGTGGCAGACTAA+TGG + chr7.3:29219351-29219370 None:intergenic 50.0%
TTGGCGTGGTGTGCAAGAAT+TGG - chr7.3:29219051-29219070 MS.gene27745:CDS 50.0%
! ACGTGTATGGGCTGTAGTCA+TGG - chr7.3:29219257-29219276 MS.gene27745:CDS 50.0%
! GTATGGGCTGTAGTCATGGT+TGG - chr7.3:29219261-29219280 MS.gene27745:CDS 50.0%
! TGTAATGGGGTCAGCAGACA+AGG + chr7.3:29219390-29219409 None:intergenic 50.0%
!! TCATGGTTGGCTTGCATTGG+TGG - chr7.3:29219274-29219293 MS.gene27745:CDS 50.0%
AGAGGTGGCAGACTAATGGG+CGG + chr7.3:29219347-29219366 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 29218963 29220075 29218963 ID=MS.gene27745
chr7.3 mRNA 29218963 29220075 29218963 ID=MS.gene27745.t1;Parent=MS.gene27745
chr7.3 exon 29218963 29220075 29218963 ID=MS.gene27745.t1.exon1;Parent=MS.gene27745.t1
chr7.3 CDS 29218963 29220075 29218963 ID=cds.MS.gene27745.t1;Parent=MS.gene27745.t1
Gene Sequence

>MS.gene27745

ATGAGGAAGGTAGACTGGGCAAATTTAGAAGCATATCCTCTTAGTTTGGTTCTGGATAAGTTGGAAGAACACATAGATCATGTTAGGTTTGGCGTGGTGTGCAAGAATTGGCTATCAATTGCAAAGCTTAACCATAAAAATCATCAATTTAGGATTAATGTACCACCTATGTTGATGATCCTCTCACATAATTGGTCTAATATATCAAAGACGGAGACGAGATCTCTGTATAGTATTATAAGTGAAAAAGAGTACCCTATTCAGATATCCCATCCTTCCATCATTGAAAGTAAAACGTGTATGGGCTGTAGTCATGGTTGGCTTGCATTGGTGGATGATAACAAAGCCATAACACTAGTGAATCCTTTTAAGTATAGTGTACCGCCCATTAGTCTGCCACCTCTCGAATCTCTCAACAAGGTTACCTTGTCTGCTGACCCCATTACAAGCCCAAGTGATTATGTGGTTGCAGCAATTTACCTTTTTAGTTTTCTCGCTTTCAAAAGACCAAGCCAATCATTTTGGAGTCATGTAGACACAAATGAATTTTATTATTTCACTGATGTTGTATTTTATAAAGGCTTGGTATTTGCCAACAGTGACGGAGACACTATTGTATCCTTCAAGCTTAACAATCCTCCTTGTGATGACTCAAATGACCCTAATTTTACTTACTATGAGAAGATAGCAAGCACTACATATATTGAACCAGAGCAATCTTATTATAGCCGCCGTGCTTATTTCGTGAAATCATTGACTGGAGACATATGGATGGTGAGAAGACGTAAAGTCGACAAACATTCATTCAAACTCCATGTTTACAAATTGGAACTGGATGTCCAGAATGGAAAACTTGAACAAATAAACAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAATCTGTGGTGATTCAACTTCTATGCCAGCCTCTTGTTTCTCCAAGTTGGAAAAGGATTCGGTTTATTTTGTTTGTGAAGGTGATAAGAAGTATTTAGGGTTAGAGATCTATAATGTGAAGGATGGAAGCTTTCGGAGACAATCTTTACCACTTTCCTTCGAGCGAATGCAACTTTTTTGGGTTCTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene27745.t1

MRKVDWANLEAYPLSLVLDKLEEHIDHVRFGVVCKNWLSIAKLNHKNHQFRINVPPMLMILSHNWSNISKTETRSLYSIISEKEYPIQISHPSIIESKTCMGCSHGWLALVDDNKAITLVNPFKYSVPPISLPPLESLNKVTLSADPITSPSDYVVAAIYLFSFLAFKRPSQSFWSHVDTNEFYYFTDVVFYKGLVFANSDGDTIVSFKLNNPPCDDSNDPNFTYYEKIASTTYIEPEQSYYSRRAYFVKSLTGDIWMVRRRKVDKHSFKLHVYKLELDVQNGKLEQINKLESLEDNILFVGICGDSTSMPASCFSKLEKDSVYFVCEGDKKYLGLEIYNVKDGSFRRQSLPLSFERMQLFWVLPQFQWD