AlfalfaGEDB | MS.gene42224

M. sativa cultivar XinJiangDaYe / MS.gene42224

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42224.t1	RHN46850.1	68.9	270	77	5	3	269	5	270	1.20E-92	349.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42224.t1	A0A396H0S2	68.9	270	77	5	3	269	5	270	8.4e-93	349.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene76061	MS.gene42224	PPI
MS.gene023891	MS.gene42224	PPI
MS.gene42224	MS.gene97317	PPI
MS.gene42224	MS.gene44542	PPI
MS.gene42224	MS.gene27747	PPI
MS.gene86085	MS.gene42224	PPI
MS.gene42224	MS.gene27754	PPI
MS.gene42224	MS.gene73460	PPI
MS.gene42224	MS.gene43191	PPI
MS.gene032784	MS.gene42224	PPI
MS.gene42224	MS.gene73726	PPI
MS.gene42224	MS.gene49592	PPI
MS.gene42224	MS.gene94239	PPI
MS.gene29654	MS.gene42224	PPI
MS.gene42224	MS.gene023892	PPI
MS.gene023785	MS.gene42224	PPI
MS.gene42224	MS.gene73723	PPI
MS.gene032780	MS.gene42224	PPI
MS.gene041287	MS.gene42224	PPI
MS.gene27745	MS.gene42224	PPI
MS.gene42224	MS.gene27637	PPI
MS.gene42224	MS.gene52254	PPI
MS.gene42224	MS.gene023890	PPI
MS.gene42224	MS.gene52262	PPI
MS.gene42224	MS.gene49594	PPI
MS.gene42224	MS.gene023888	PPI
MS.gene29651	MS.gene42224	PPI
MS.gene42224	MS.gene52259	PPI
MS.gene023787	MS.gene42224	PPI
MS.gene032785	MS.gene42224	PPI
MS.gene27751	MS.gene42224	PPI
MS.gene42224	MS.gene27639	PPI
MS.gene42224	MS.gene22627	PPI
MS.gene49593	MS.gene42224	PPI
MS.gene066291	MS.gene42224	PPI
MS.gene42224	MS.gene46208	PPI
MS.gene066292	MS.gene42224	PPI
MS.gene023889	MS.gene42224	PPI
MS.gene029514	MS.gene42224	PPI
MS.gene022086	MS.gene42224	PPI
MS.gene42224	MS.gene95450	PPI
MS.gene27749	MS.gene42224	PPI
MS.gene52258	MS.gene42224	PPI
MS.gene42222	MS.gene42224	PPI
MS.gene99022	MS.gene42224	PPI
MS.gene023893	MS.gene42224	PPI
MS.gene42224	MS.gene49593	PPI
MS.gene27750	MS.gene42224	PPI
MS.gene52697	MS.gene42224	PPI
MS.gene42224	MS.gene42429	PPI
MS.gene42224	MS.gene42431	PPI
MS.gene42224	MS.gene52261	PPI
MS.gene42224	MS.gene031137	PPI
MS.gene42224	MS.gene031136	PPI
MS.gene42224	MS.gene42226	PPI
MS.gene42430	MS.gene42224	PPI
MS.gene42224	MS.gene52166	PPI
MS.gene73723	MS.gene42224	PPI
MS.gene42224	MS.gene52164	PPI
MS.gene066289	MS.gene42224	PPI
MS.gene42224	MS.gene023887	PPI
MS.gene42224	MS.gene76059	PPI
MS.gene99024	MS.gene42224	PPI
MS.gene52163	MS.gene42224	PPI
MS.gene42224	MS.gene85835	PPI
MS.gene42224	MS.gene032785	PPI
MS.gene94239	MS.gene42224	PPI
MS.gene42224	MS.gene42433	PPI
MS.gene99023	MS.gene42224	PPI
MS.gene035137	MS.gene42224	PPI
MS.gene42224	MS.gene42432	PPI
MS.gene42224	MS.gene27635	PPI
MS.gene52253	MS.gene42224	PPI
MS.gene42224	MS.gene52913	PPI
MS.gene42224	MS.gene27748	PPI
MS.gene42224	MS.gene27751	PPI
MS.gene42224	MS.gene42223	PPI
MS.gene42224	MS.gene42428	PPI
MS.gene42224	MS.gene023784	PPI
MS.gene027042	MS.gene42224	PPI
MS.gene035135	MS.gene42224	PPI
MS.gene42224	MS.gene52263	PPI
MS.gene42224	MS.gene76056	PPI
MS.gene52255	MS.gene42224	PPI
MS.gene42224	MS.gene85826	PPI
MS.gene94237	MS.gene42224	PPI
MS.gene73460	MS.gene42224	PPI
MS.gene27753	MS.gene42224	PPI
MS.gene42224	MS.gene27636	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42224.t1	MTR_7g077620	65.056	269	88	5	1	266	104	369	1.42e-109	322
MS.gene42224.t1	MTR_7g076100	70.386	233	64	4	40	269	70	300	3.32e-105	307
MS.gene42224.t1	MTR_7g077660	61.679	274	93	7	1	269	102	368	4.56e-101	299
MS.gene42224.t1	MTR_7g077650	59.643	280	92	8	1	269	103	372	9.21e-101	299
MS.gene42224.t1	MTR_7g076110	57.246	276	108	4	3	269	39	313	1.03e-97	289
MS.gene42224.t1	MTR_7g076130	75.806	124	27	2	66	186	25	148	5.61e-60	187
MS.gene42224.t1	MTR_5g038260	41.667	300	129	16	3	269	97	383	1.06e-49	168
MS.gene42224.t1	MTR_1g108740	42.491	273	123	11	3	267	98	344	4.53e-48	163
MS.gene42224.t1	MTR_5g038230	40.136	294	112	14	3	267	95	353	6.09e-44	152
MS.gene42224.t1	MTR_5g082780	40.948	232	95	10	11	224	1	208	7.57e-43	146
MS.gene42224.t1	MTR_1g064570	35.016	317	145	13	3	269	95	400	1.04e-39	142
MS.gene42224.t1	MTR_5g082770	42.584	209	99	7	3	201	91	288	5.43e-37	132
MS.gene42224.t1	MTR_1g016150	46.465	99	50	2	3	100	107	203	6.28e-18	80.9
MS.gene42224.t1	MTR_1g016150	58.462	65	21	2	151	215	177	235	6.19e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 48 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATCATTTGGACTTGTAAT+TGG	0.148563	7.3:+29657609	None:intergenic
TTCGCATAGTAAGTAAAATT+AGG	0.191950	7.3:+29657399	None:intergenic
AAAGAGCAAGCCAACCATTT+TGG	0.203122	7.3:-29657545	MS.gene42224:CDS
GATATACTACTACACTTAAA+AGG	0.276632	7.3:+29657687	None:intergenic
AATGAACAAGCTTGAGAGTT+TGG	0.278023	7.3:-29657193	MS.gene42224:CDS
ATTTGGTTGGTTTGCATTAG+TGG	0.297529	7.3:-29657739	MS.gene42224:CDS
AAGATCACCAATGTTGTAAA+TGG	0.306746	7.3:+29657575	None:intergenic
GTTGAGATGTGGCAGAATAA+TGG	0.333023	7.3:+29657662	None:intergenic
TCGCATAGTAAGTAAAATTA+GGG	0.335896	7.3:+29657400	None:intergenic
TTGAGATGTGGCAGAATAAT+GGG	0.362137	7.3:+29657663	None:intergenic
GTTGAACTTGAAGGATATAA+TGG	0.375803	7.3:+29657443	None:intergenic
AAATTAGGGTTATTTGAGTT+AGG	0.386206	7.3:+29657414	None:intergenic
CGCATCCCCTTGAAGGAAAT+CGG	0.389019	7.3:+29657000	None:intergenic
AATCATTTGGACTTGTAATT+GGG	0.394312	7.3:+29657610	None:intergenic
AAAACCTCGCATCCCCTTGA+AGG	0.402319	7.3:+29656993	None:intergenic
GGGTTCGACCGCAATTTCAA+TGG	0.414532	7.3:-29656969	MS.gene42224:CDS
AAAAGGATTGACTAGAGTTA+TGG	0.415743	7.3:+29657704	None:intergenic
AGGTGTATGGACTCTTCATT+TGG	0.419747	7.3:-29657756	None:intergenic
GTAGACATCAAGTTTATATG+AGG	0.436334	7.3:+29657254	None:intergenic
GTATGGACTCTTCATTTGGT+TGG	0.436531	7.3:-29657752	MS.gene42224:CDS
TGATGTCTACAAATTAGAAC+TGG	0.443705	7.3:-29657241	MS.gene42224:CDS
TGTGCTATATGAATCCAAAA+TGG	0.443882	7.3:+29657531	None:intergenic
GCTGCAATGACATAATCATT+TGG	0.444853	7.3:+29657597	None:intergenic
GGTTCGACCGCAATTTCAAT+GGG	0.479149	7.3:-29656968	MS.gene42224:CDS
AGGAGGATTGTTGAACTTGA+AGG	0.483112	7.3:+29657434	None:intergenic
TGAGAAGATATTTCATCAAT+CGG	0.493569	7.3:-29657287	MS.gene42224:CDS
AATCATTGAATGGAGACATA+TGG	0.507395	7.3:-29657314	MS.gene42224:CDS
ATTTATGACCATGGTATGCA+TGG	0.513250	7.3:-29657078	MS.gene42224:CDS
ATTGCAGCCATTTACAACAT+TGG	0.532590	7.3:-29657582	MS.gene42224:CDS
GCAGACAAGGTAACCTTGTA+GGG	0.532760	7.3:+29657636	None:intergenic
TTGCTATTTGCCGACTGTGA+AGG	0.537297	7.3:-29657471	MS.gene42224:CDS
TTAGGGTTATTTGAGTTAGG+AGG	0.546912	7.3:+29657417	None:intergenic
AGCAGACAAGGTAACCTTGT+AGG	0.548503	7.3:+29657635	None:intergenic
ACATCTCAACTCTCCCTACA+AGG	0.555243	7.3:-29657649	MS.gene42224:CDS
GAGCTCTATAATGCGAAGGA+TGG	0.571019	7.3:-29657045	MS.gene42224:CDS
TTCTAACTTGGAGAAACGAG+AGG	0.573200	7.3:+29657119	None:intergenic
TGTAATTGGGTCAGCAGACA+AGG	0.582291	7.3:+29657623	None:intergenic
TATCAATCCCATTGAAATTG+CGG	0.600714	7.3:+29656961	None:intergenic
ATTTCCTTCAAGGGGATGCG+AGG	0.607717	7.3:-29656997	MS.gene42224:CDS
CTATATGAATCCAAAATGGT+TGG	0.609689	7.3:+29657535	None:intergenic
AATGGTGTGTCCTTCACAGT+CGG	0.630964	7.3:+29657461	None:intergenic
GTTAGAGCTCTATAATGCGA+AGG	0.632407	7.3:-29657049	MS.gene42224:CDS
ACTCTAAACCATGCATACCA+TGG	0.661226	7.3:+29657070	None:intergenic
TTGTAGGGAGAGTTGAGATG+TGG	0.664401	7.3:+29657651	None:intergenic
ATGCGAAGATAGCTAGCACA+AGG	0.689632	7.3:-29657383	MS.gene42224:CDS
ATTGAATGGAGACATATGGA+TGG	0.693585	7.3:-29657310	MS.gene42224:CDS
TTAGAACTGGATGCCCAGAG+TGG	0.702949	7.3:-29657228	MS.gene42224:CDS
ACACAGAAACTGAATCACCA+TGG	0.719525	7.3:+29657145	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATAATGTTGTATTTTATAA+AGG	-	chr7.3:29657218-29657237	MS.gene42224:CDS	10.0%
!!!	ATTTATTTTATTTATGACCA+TGG	-	chr7.3:29657626-29657645	MS.gene42224:CDS	15.0%
!!!	AAATCTTTTCATAATAGCAT+TGG	+	chr7.3:29657370-29657389	None:intergenic	20.0%
!!!	AATCTTTTCATAATAGCATT+GGG	+	chr7.3:29657369-29657388	None:intergenic	20.0%
!!!	TATTTTGTGAAATCATTGAA+TGG	-	chr7.3:29657389-29657408	MS.gene42224:CDS	20.0%
!	AATCATTTGGACTTGTAATT+GGG	+	chr7.3:29657106-29657125	None:intergenic	25.0%
!	GATATACTACTACACTTAAA+AGG	+	chr7.3:29657029-29657048	None:intergenic	25.0%
!	TAATCATTTGGACTTGTAAT+TGG	+	chr7.3:29657107-29657126	None:intergenic	25.0%
!	TCGCATAGTAAGTAAAATTA+GGG	+	chr7.3:29657316-29657335	None:intergenic	25.0%
!	TGAGAAGATATTTCATCAAT+CGG	-	chr7.3:29657426-29657445	MS.gene42224:CDS	25.0%
!	TTCGCATAGTAAGTAAAATT+AGG	+	chr7.3:29657317-29657336	None:intergenic	25.0%
!!	AAATTAGGGTTATTTGAGTT+AGG	+	chr7.3:29657302-29657321	None:intergenic	25.0%
!!	AATCGAATCTTTTTCTAACT+TGG	+	chr7.3:29657609-29657628	None:intergenic	25.0%
!!!	GAAGACAACATTTTATTTGT+CGG	-	chr7.3:29657542-29657561	MS.gene42224:CDS	25.0%
	AAAAGGATTGACTAGAGTTA+TGG	+	chr7.3:29657012-29657031	None:intergenic	30.0%
	AAGATCACCAATGTTGTAAA+TGG	+	chr7.3:29657141-29657160	None:intergenic	30.0%
	AATCATTGAATGGAGACATA+TGG	-	chr7.3:29657399-29657418	MS.gene42224:CDS	30.0%
	CTATATGAATCCAAAATGGT+TGG	+	chr7.3:29657181-29657200	None:intergenic	30.0%
	GTAGACATCAAGTTTATATG+AGG	+	chr7.3:29657462-29657481	None:intergenic	30.0%
	GTTGAACTTGAAGGATATAA+TGG	+	chr7.3:29657273-29657292	None:intergenic	30.0%
	TGATGTCTACAAATTAGAAC+TGG	-	chr7.3:29657472-29657491	MS.gene42224:CDS	30.0%
	TGTGCTATATGAATCCAAAA+TGG	+	chr7.3:29657185-29657204	None:intergenic	30.0%
!!	ATTTTATTTGTCGGAAACCA+TGG	-	chr7.3:29657551-29657570	MS.gene42224:CDS	30.0%
!!	TTTTCATAATAGCATTGGGA+TGG	+	chr7.3:29657365-29657384	None:intergenic	30.0%
!!!	ATTTTTACCGATTTCCTTCA+AGG	-	chr7.3:29657706-29657725	MS.gene42224:CDS	30.0%
!!!	TTTTTACCGATTTCCTTCAA+GGG	-	chr7.3:29657707-29657726	MS.gene42224:CDS	30.0%
	AATGAACAAGCTTGAGAGTT+TGG	-	chr7.3:29657520-29657539	MS.gene42224:CDS	35.0%
	ATTGAATGGAGACATATGGA+TGG	-	chr7.3:29657403-29657422	MS.gene42224:CDS	35.0%
	ATTGCAGCCATTTACAACAT+TGG	-	chr7.3:29657131-29657150	MS.gene42224:CDS	35.0%
	ATTTATGACCATGGTATGCA+TGG	-	chr7.3:29657635-29657654	MS.gene42224:CDS	35.0%
	GCTGCAATGACATAATCATT+TGG	+	chr7.3:29657119-29657138	None:intergenic	35.0%
	TTGAGATGTGGCAGAATAAT+GGG	+	chr7.3:29657053-29657072	None:intergenic	35.0%
!	TTAGGGTTATTTGAGTTAGG+AGG	+	chr7.3:29657299-29657318	None:intergenic	35.0%
!!	ATTTGGTTGGTTTGCATTAG+TGG	-	chr7.3:29656974-29656993	MS.gene42224:CDS	35.0%
!!	TTGTTCAAGTTTTCCACTCT+GGG	+	chr7.3:29657501-29657520	None:intergenic	35.0%
!!	TTTGTTCAAGTTTTCCACTC+TGG	+	chr7.3:29657502-29657521	None:intergenic	35.0%
!!!	TTTTACCGATTTCCTTCAAG+GGG	-	chr7.3:29657708-29657727	MS.gene42224:CDS	35.0%
	ACACAGAAACTGAATCACCA+TGG	+	chr7.3:29657571-29657590	None:intergenic	40.0%
	ACTCTAAACCATGCATACCA+TGG	+	chr7.3:29657646-29657665	None:intergenic	40.0%
	AGGAGGATTGTTGAACTTGA+AGG	+	chr7.3:29657282-29657301	None:intergenic	40.0%
	GTTAGAGCTCTATAATGCGA+AGG	-	chr7.3:29657664-29657683	MS.gene42224:CDS	40.0%
	GTTGAGATGTGGCAGAATAA+TGG	+	chr7.3:29657054-29657073	None:intergenic	40.0%
	TTCTAACTTGGAGAAACGAG+AGG	+	chr7.3:29657597-29657616	None:intergenic	40.0%
!	AAAGAGCAAGCCAACCATTT+TGG	-	chr7.3:29657168-29657187	MS.gene42224:CDS	40.0%
	AATGGTGTGTCCTTCACAGT+CGG	+	chr7.3:29657255-29657274	None:intergenic	45.0%
	ACATCTCAACTCTCCCTACA+AGG	-	chr7.3:29657064-29657083	MS.gene42224:CDS	45.0%
	AGCAGACAAGGTAACCTTGT+AGG	+	chr7.3:29657081-29657100	None:intergenic	45.0%
	ATGCGAAGATAGCTAGCACA+AGG	-	chr7.3:29657330-29657349	MS.gene42224:CDS	45.0%
	GAGCTCTATAATGCGAAGGA+TGG	-	chr7.3:29657668-29657687	MS.gene42224:CDS	45.0%
	GCAGACAAGGTAACCTTGTA+GGG	+	chr7.3:29657080-29657099	None:intergenic	45.0%
	GGTTCGACCGCAATTTCAAT+GGG	-	chr7.3:29657745-29657764	MS.gene42224:CDS	45.0%
	TGTAATTGGGTCAGCAGACA+AGG	+	chr7.3:29657093-29657112	None:intergenic	45.0%
	TTGTAGGGAGAGTTGAGATG+TGG	+	chr7.3:29657065-29657084	None:intergenic	45.0%
!!	TTGCTATTTGCCGACTGTGA+AGG	-	chr7.3:29657242-29657261	MS.gene42224:CDS	45.0%
	AAAACCTCGCATCCCCTTGA+AGG	+	chr7.3:29657723-29657742	None:intergenic	50.0%
	ATTTCCTTCAAGGGGATGCG+AGG	-	chr7.3:29657716-29657735	MS.gene42224:CDS	50.0%
	CGCATCCCCTTGAAGGAAAT+CGG	+	chr7.3:29657716-29657735	None:intergenic	50.0%
	GGGTTCGACCGCAATTTCAA+TGG	-	chr7.3:29657744-29657763	MS.gene42224:CDS	50.0%
	TTAGAACTGGATGCCCAGAG+TGG	-	chr7.3:29657485-29657504	MS.gene42224:CDS	50.0%
!	CAAGGGGATGCGAGGTTTTT+GGG	-	chr7.3:29657724-29657743	MS.gene42224:CDS	50.0%
!	TCAAGGGGATGCGAGGTTTT+TGG	-	chr7.3:29657723-29657742	MS.gene42224:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	29656963	29657772	29656963	ID=MS.gene42224
chr7.3	mRNA	29656963	29657772	29656963	ID=MS.gene42224.t1;Parent=MS.gene42224
chr7.3	exon	29656963	29657772	29656963	ID=MS.gene42224.t1.exon1;Parent=MS.gene42224.t1
chr7.3	CDS	29656963	29657772	29656963	ID=cds.MS.gene42224.t1;Parent=MS.gene42224.t1

Gene Sequence

>MS.gene42224

ATGGACTCTTCATTTGGTTGGTTTGCATTAGTGGACAATAACAATGCCATAACTCTAGTCAATCCTTTTAAGTGTAGTAGTATATCTCCCATTATTCTGCCACATCTCAACTCTCCCTACAAGGTTACCTTGTCTGCTGACCCAATTACAAGTCCAAATGATTATGTCATTGCAGCCATTTACAACATTGGTGATCTTGCTTTCAAAAGAGCAAGCCAACCATTTTGGATTCATATAGCACATGAATTTGCTATCAATAATGTTGTATTTTATAAAGGCTTGCTATTTGCCGACTGTGAAGGACACACCATTATATCCTTCAAGTTCAACAATCCTCCTAACTCAAATAACCCTAATTTTACTTACTATGCGAAGATAGCTAGCACAAGGTATAGTGTTCCATCCCAATGCTATTATGAAAAGATTTATTTTGTGAAATCATTGAATGGAGACATATGGATGGTGAGAAGATATTTCATCAATCGGTATGACAAGTCCTCATATAAACTTGATGTCTACAAATTAGAACTGGATGCCCAGAGTGGAAAACTTGAACAAATGAACAAGCTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAAACCATGGTGATTCAGTTTCTGTGTCAGCCTCTCGTTTCTCCAAGTTAGAAAAAGATTCGATTTATTTTATTTATGACCATGGTATGCATGGTTTAGAGTTAGAGCTCTATAATGCGAAGGATGGAAGCTGTCAGAGACAATTTTTACCGATTTCCTTCAAGGGGATGCGAGGTTTTTGGGTTCGACCGCAATTTCAATGGGATTGA

Protein sequence

>MS.gene42224.t1

MDSSFGWFALVDNNNAITLVNPFKCSSISPIILPHLNSPYKVTLSADPITSPNDYVIAAIYNIGDLAFKRASQPFWIHIAHEFAINNVVFYKGLLFADCEGHTIISFKFNNPPNSNNPNFTYYAKIASTRYSVPSQCYYEKIYFVKSLNGDIWMVRRYFINRYDKSSYKLDVYKLELDAQSGKLEQMNKLESLEDNILFVGNHGDSVSVSASRFSKLEKDSIYFIYDHGMHGLELELYNAKDGSCQRQFLPISFKGMRGFWVRPQFQWD