AlfalfaGEDB | MS.gene52261

M. sativa cultivar XinJiangDaYe / MS.gene52261

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52261.t1	AES80177.2	71.6	275	72	2	1	270	99	372	4.00E-101	377.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52261.t1	G7L4M9	71.6	275	72	2	1	270	99	372	2.9e-101	377.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene52258	MS.gene52261	PPI
MS.gene52261	MS.gene95450	PPI
MS.gene85826	MS.gene52261	PPI
MS.gene42222	MS.gene52261	PPI
MS.gene99022	MS.gene52261	PPI
MS.gene52164	MS.gene52261	PPI
MS.gene023893	MS.gene52261	PPI
MS.gene27636	MS.gene52261	PPI
MS.gene27750	MS.gene52261	PPI
MS.gene52697	MS.gene52261	PPI
MS.gene27747	MS.gene52261	PPI
MS.gene52261	MS.gene42429	PPI
MS.gene85835	MS.gene52261	PPI
MS.gene031137	MS.gene52261	PPI
MS.gene52261	MS.gene031137	PPI
MS.gene52261	MS.gene22627	PPI
MS.gene52261	MS.gene031136	PPI
MS.gene27637	MS.gene52261	PPI
MS.gene42430	MS.gene52261	PPI
MS.gene023888	MS.gene52261	PPI
MS.gene066292	MS.gene52261	PPI
MS.gene73723	MS.gene52261	PPI
MS.gene52261	MS.gene52164	PPI
MS.gene066289	MS.gene52261	PPI
MS.gene52261	MS.gene023887	PPI
MS.gene023784	MS.gene52261	PPI
MS.gene52261	MS.gene76059	PPI
MS.gene76056	MS.gene52261	PPI
MS.gene42226	MS.gene52261	PPI
MS.gene22627	MS.gene52261	PPI
MS.gene99024	MS.gene52261	PPI
MS.gene52163	MS.gene52261	PPI
MS.gene42431	MS.gene52261	PPI
MS.gene52261	MS.gene85835	PPI
MS.gene94239	MS.gene52261	PPI
MS.gene42429	MS.gene52261	PPI
MS.gene99023	MS.gene52261	PPI
MS.gene035137	MS.gene52261	PPI
MS.gene52261	MS.gene42432	PPI
MS.gene52261	MS.gene27635	PPI
MS.gene52253	MS.gene52261	PPI
MS.gene44542	MS.gene52261	PPI
MS.gene023892	MS.gene52261	PPI
MS.gene52261	MS.gene52913	PPI
MS.gene52261	MS.gene27748	PPI
MS.gene52262	MS.gene52261	PPI
MS.gene27748	MS.gene52261	PPI
MS.gene52261	MS.gene42223	PPI
MS.gene73726	MS.gene52261	PPI
MS.gene52261	MS.gene42428	PPI
MS.gene52166	MS.gene52261	PPI
MS.gene027042	MS.gene52261	PPI
MS.gene52259	MS.gene52261	PPI
MS.gene035135	MS.gene52261	PPI
MS.gene52255	MS.gene52261	PPI
MS.gene52261	MS.gene85826	PPI
MS.gene52263	MS.gene52261	PPI
MS.gene94237	MS.gene52261	PPI
MS.gene73460	MS.gene52261	PPI
MS.gene27753	MS.gene52261	PPI
MS.gene49594	MS.gene52261	PPI
MS.gene023891	MS.gene52261	PPI
MS.gene52261	MS.gene97317	PPI
MS.gene42224	MS.gene52261	PPI
MS.gene032784	MS.gene52261	PPI
MS.gene52261	MS.gene49592	PPI
MS.gene42223	MS.gene52261	PPI
MS.gene29654	MS.gene52261	PPI
MS.gene023785	MS.gene52261	PPI
MS.gene032780	MS.gene52261	PPI
MS.gene041287	MS.gene52261	PPI
MS.gene27745	MS.gene52261	PPI
MS.gene52261	MS.gene27637	PPI
MS.gene42433	MS.gene52261	PPI
MS.gene43191	MS.gene52261	PPI
MS.gene52261	MS.gene52254	PPI
MS.gene52261	MS.gene023890	PPI
MS.gene29651	MS.gene52261	PPI
MS.gene46208	MS.gene52261	PPI
MS.gene76061	MS.gene52261	PPI
MS.gene52261	MS.gene52259	PPI
MS.gene023787	MS.gene52261	PPI
MS.gene86085	MS.gene52261	PPI
MS.gene032785	MS.gene52261	PPI
MS.gene27751	MS.gene52261	PPI
MS.gene52261	MS.gene27639	PPI
MS.gene52254	MS.gene52261	PPI
MS.gene49593	MS.gene52261	PPI
MS.gene066291	MS.gene52261	PPI
MS.gene023889	MS.gene52261	PPI
MS.gene029514	MS.gene52261	PPI
MS.gene022086	MS.gene52261	PPI
MS.gene27754	MS.gene52261	PPI
MS.gene023887	MS.gene52261	PPI
MS.gene27749	MS.gene52261	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52261.t1	MTR_7g077650	71.636	275	72	2	1	270	99	372	3.15e-131	376
MS.gene52261.t1	MTR_7g077660	72.059	272	71	4	2	270	99	368	1.96e-128	369
MS.gene52261.t1	MTR_7g077620	69.630	270	75	4	2	266	101	368	5.85e-126	363
MS.gene52261.t1	MTR_7g076110	54.874	277	112	6	6	270	38	313	5.17e-92	275
MS.gene52261.t1	MTR_7g076100	60.924	238	84	5	39	270	66	300	1.39e-88	265
MS.gene52261.t1	MTR_1g108740	46.097	269	118	12	6	268	97	344	8.37e-58	188
MS.gene52261.t1	MTR_5g038260	41.447	304	131	12	2	270	92	383	2.85e-53	177
MS.gene52261.t1	MTR_5g038230	41.606	274	97	10	2	246	90	329	2.12e-48	164
MS.gene52261.t1	MTR_5g082770	45.116	215	89	5	6	206	90	289	7.77e-44	150
MS.gene52261.t1	MTR_7g076130	57.600	125	49	2	69	190	25	148	1.55e-40	137
MS.gene52261.t1	MTR_1g064570	35.890	326	137	13	2	270	90	400	1.32e-39	142
MS.gene52261.t1	MTR_5g082780	37.768	233	113	8	15	234	1	214	3.93e-37	131
MS.gene52261.t1	MTR_1g016150	48.193	83	39	3	6	85	106	187	2.73e-15	73.6
MS.gene52261.t1	MTR_6g023955	42.857	98	52	3	41	137	73	167	4.03e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGAGTGGATGCATCATTTC+TGG	0.085063	7.4:-28487811	MS.gene52261:CDS
AGGAGGATTGTTTAGCTTTA+AGG	0.194494	7.4:+28488246	None:intergenic
ATGAATGATCATATGGATTC+AGG	0.202366	7.4:-28487893	MS.gene52261:CDS
AAAGAGCAAGCCAATCATTT+TGG	0.202770	7.4:-28488360	MS.gene52261:CDS
AATGAACAAACTTGAGAGTT+TGG	0.291432	7.4:-28488005	MS.gene52261:CDS
CCATCATAACAGCGTAGTTT+TGG	0.316315	7.4:+28488157	None:intergenic
ACCTCTCGAATCTCTCAAAA+AGG	0.330053	7.4:-28488464	MS.gene52261:CDS
GGAGGAATACTACACTTAAA+AGG	0.332852	7.4:+28488499	None:intergenic
CGAGTGGATGCATCATTTCT+GGG	0.345780	7.4:-28487810	MS.gene52261:CDS
GATTTATATGAATGATCATA+TGG	0.372503	7.4:-28487900	MS.gene52261:CDS
TTCGAGAGGTGGCAGACTAA+TGG	0.374836	7.4:+28488477	None:intergenic
CCATGGTTGGCTTGCATTGG+TGG	0.397590	7.4:-28488551	MS.gene52261:CDS
TTGTTTCTCCACATTGGAAA+AGG	0.424044	7.4:-28487927	MS.gene52261:CDS
TCGAGAGGTGGCAGACTAAT+GGG	0.438780	7.4:+28488478	None:intergenic
GGGTTCTGCCACAATGTCAA+TGG	0.441194	7.4:-28487790	MS.gene52261:CDS
ACTAGCCATCTTCTCATAGT+AGG	0.443005	7.4:+28488195	None:intergenic
TGAATGATCATATGGATTCA+GGG	0.448866	7.4:-28487892	MS.gene52261:CDS
TAGCCATGGTTGGCTTGCAT+TGG	0.461010	7.4:-28488554	MS.gene52261:CDS
CCAAAACTACGCTGTTATGA+TGG	0.471568	7.4:-28488157	MS.gene52261:CDS
CTACACGAATCCAAAATGAT+TGG	0.482901	7.4:+28488350	None:intergenic
AGCCTCTTGTTTCTCCACAT+TGG	0.483410	7.4:-28487933	MS.gene52261:CDS
TAATCACTTGGATTTGTCAT+GGG	0.490766	7.4:+28488424	None:intergenic
AGAATCTATAATGTGAAGGA+TGG	0.493671	7.4:-28487866	MS.gene52261:CDS
TCTTACCGCTTTCCTTCGAG+TGG	0.511644	7.4:-28487826	MS.gene52261:CDS
TGAGAAGATGCTTAATCGAC+GGG	0.520399	7.4:-28488090	MS.gene52261:CDS
AATCATTGACCGGAGACATA+TGG	0.521122	7.4:-28488117	MS.gene52261:CDS
GGTTCTGCCACAATGTCAAT+GGG	0.526211	7.4:-28487789	MS.gene52261:CDS
AATGATTTCACAAAATAAGC+AGG	0.533478	7.4:+28488133	None:intergenic
ATAATCACTTGGATTTGTCA+TGG	0.537229	7.4:+28488423	None:intergenic
TGTCATGGGGTCAGCAGACA+AGG	0.556574	7.4:+28488438	None:intergenic
TCTCATCAACCATATGTCTC+CGG	0.558876	7.4:+28488108	None:intergenic
ATGAGAAGATGCTTAATCGA+CGG	0.569894	7.4:-28488091	MS.gene52261:CDS
GACAAATCCAAGTGATTATG+TGG	0.574598	7.4:-28488419	MS.gene52261:CDS
TTGGAACTGAATGCCGAGAG+TGG	0.585480	7.4:-28488040	MS.gene52261:CDS
CGATCAATTGAGTCATCAGA+AGG	0.588157	7.4:+28488226	None:intergenic
GTTTAGCTTTAAGGATACAA+TGG	0.589562	7.4:+28488255	None:intergenic
GCTGCAACCACATAATCACT+TGG	0.591162	7.4:+28488412	None:intergenic
AATCACTTGGATTTGTCATG+GGG	0.596697	7.4:+28488425	None:intergenic
GATCAATCCCATTGACATTG+TGG	0.599138	7.4:+28487782	None:intergenic
GTTAAGAATCTATAATGTGA+AGG	0.604544	7.4:-28487870	MS.gene52261:CDS
GAAATGATGCATCCACTCGA+AGG	0.618187	7.4:+28487814	None:intergenic
TTCCAATGTGGAGAAACAAG+AGG	0.648839	7.4:+28487931	None:intergenic
TGCATCCACTCGAAGGAAAG+CGG	0.655155	7.4:+28487821	None:intergenic
TCAATTGAGTCATCAGAAGG+AGG	0.655171	7.4:+28488229	None:intergenic
CCACCAATGCAAGCCAACCA+TGG	0.665097	7.4:+28488551	None:intergenic
AGAGGTGGCAGACTAATGGG+AGG	0.718242	7.4:+28488481	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAGTGAAAAAAAATTCAT+TGG	+	chr7.4:28488037-28488056	None:intergenic	15.0%
!!!	ACTAATGTTGTATTTTATAA+AGG	-	chr7.4:28488051-28488070	MS.gene52261:CDS	15.0%
!!	GATTTATATGAATGATCATA+TGG	-	chr7.4:28488458-28488477	MS.gene52261:CDS	20.0%
!	AATGATTTCACAAAATAAGC+AGG	+	chr7.4:28488228-28488247	None:intergenic	25.0%
!	GTTAAGAATCTATAATGTGA+AGG	-	chr7.4:28488488-28488507	MS.gene52261:CDS	25.0%
!!	TATTTTGTGAAATCATTGAC+CGG	-	chr7.4:28488231-28488250	MS.gene52261:CDS	25.0%
!!	TGTTGTATTTTATAAAGGCT+TGG	-	chr7.4:28488056-28488075	MS.gene52261:CDS	25.0%
!!!	GAAGACAACATTTTATTTGT+CGG	-	chr7.4:28488375-28488394	MS.gene52261:CDS	25.0%
	AATGAACAAACTTGAGAGTT+TGG	-	chr7.4:28488353-28488372	MS.gene52261:CDS	30.0%
	AGAATCTATAATGTGAAGGA+TGG	-	chr7.4:28488492-28488511	MS.gene52261:CDS	30.0%
	ATAATCACTTGGATTTGTCA+TGG	+	chr7.4:28487938-28487957	None:intergenic	30.0%
	ATGAATGATCATATGGATTC+AGG	-	chr7.4:28488465-28488484	MS.gene52261:CDS	30.0%
	TAATCACTTGGATTTGTCAT+GGG	+	chr7.4:28487937-28487956	None:intergenic	30.0%
	TGAATGATCATATGGATTCA+GGG	-	chr7.4:28488466-28488485	MS.gene52261:CDS	30.0%
!	CAAAATGCACGTTTTCAAAT+TGG	-	chr7.4:28488299-28488318	MS.gene52261:CDS	30.0%
!	TTTTTCCTACTATGAGAAGA+TGG	-	chr7.4:28488158-28488177	MS.gene52261:CDS	30.0%
!!	ATTTTATTTGTCGGAAGTGT+TGG	-	chr7.4:28488384-28488403	MS.gene52261:CDS	30.0%
!!	GTTTAGCTTTAAGGATACAA+TGG	+	chr7.4:28488106-28488125	None:intergenic	30.0%
!!!	CGTAGTTTTGGAATCATATA+TGG	+	chr7.4:28488192-28488211	None:intergenic	30.0%
	AATCACTTGGATTTGTCATG+GGG	+	chr7.4:28487936-28487955	None:intergenic	35.0%
	ATGAGAAGATGCTTAATCGA+CGG	-	chr7.4:28488267-28488286	MS.gene52261:CDS	35.0%
	CTACACGAATCCAAAATGAT+TGG	+	chr7.4:28488011-28488030	None:intergenic	35.0%
	GACAAATCCAAGTGATTATG+TGG	-	chr7.4:28487939-28487958	MS.gene52261:CDS	35.0%
	GGAGGAATACTACACTTAAA+AGG	+	chr7.4:28487862-28487881	None:intergenic	35.0%
!	AAAGAGCAAGCCAATCATTT+TGG	-	chr7.4:28487998-28488017	MS.gene52261:CDS	35.0%
!	AATCGAATCCTTTTCCAATG+TGG	+	chr7.4:28488442-28488461	None:intergenic	35.0%
!	TTGTTTCTCCACATTGGAAA+AGG	-	chr7.4:28488431-28488450	MS.gene52261:CDS	35.0%
!!	AGGAGGATTGTTTAGCTTTA+AGG	+	chr7.4:28488115-28488134	None:intergenic	35.0%
!!	TTGTTCAAGTTTTCCACTCT+CGG	+	chr7.4:28488334-28488353	None:intergenic	35.0%
	AATCATTGACCGGAGACATA+TGG	-	chr7.4:28488241-28488260	MS.gene52261:CDS	40.0%
	ACCTCTCGAATCTCTCAAAA+AGG	-	chr7.4:28487894-28487913	MS.gene52261:CDS	40.0%
	ACTAGCCATCTTCTCATAGT+AGG	+	chr7.4:28488166-28488185	None:intergenic	40.0%
	CCAAAACTACGCTGTTATGA+TGG	-	chr7.4:28488201-28488220	MS.gene52261:CDS	40.0%
	CGATCAATTGAGTCATCAGA+AGG	+	chr7.4:28488135-28488154	None:intergenic	40.0%
	TCAATTGAGTCATCAGAAGG+AGG	+	chr7.4:28488132-28488151	None:intergenic	40.0%
	TCTCATCAACCATATGTCTC+CGG	+	chr7.4:28488253-28488272	None:intergenic	40.0%
	TGAGAAGATGCTTAATCGAC+GGG	-	chr7.4:28488268-28488287	MS.gene52261:CDS	40.0%
	TTCCAATGTGGAGAAACAAG+AGG	+	chr7.4:28488430-28488449	None:intergenic	40.0%
!	CCATCATAACAGCGTAGTTT+TGG	+	chr7.4:28488204-28488223	None:intergenic	40.0%
!!	ACCTTTTTGAGAGATTCGAG+AGG	+	chr7.4:28487898-28487917	None:intergenic	40.0%
!!	TTTTTGAGAGATTCGAGAGG+TGG	+	chr7.4:28487895-28487914	None:intergenic	40.0%
	CGAGTGGATGCATCATTTCT+GGG	-	chr7.4:28488548-28488567	MS.gene52261:CDS	45.0%
	GAAATGATGCATCCACTCGA+AGG	+	chr7.4:28488547-28488566	None:intergenic	45.0%
	GCTGCAACCACATAATCACT+TGG	+	chr7.4:28487949-28487968	None:intergenic	45.0%
	GGTTCTGCCACAATGTCAAT+GGG	-	chr7.4:28488569-28488588	MS.gene52261:CDS	45.0%
	TCGAGTGGATGCATCATTTC+TGG	-	chr7.4:28488547-28488566	MS.gene52261:CDS	45.0%
!	AGCCTCTTGTTTCTCCACAT+TGG	-	chr7.4:28488425-28488444	MS.gene52261:CDS	45.0%
!!!	TTGTTTTTGGGCTGTAGCCA+TGG	-	chr7.4:28487790-28487809	MS.gene52261:CDS	45.0%
	GGGTTCTGCCACAATGTCAA+TGG	-	chr7.4:28488568-28488587	MS.gene52261:CDS	50.0%
	TCGAGAGGTGGCAGACTAAT+GGG	+	chr7.4:28487883-28487902	None:intergenic	50.0%
	TCTTACCGCTTTCCTTCGAG+TGG	-	chr7.4:28488532-28488551	MS.gene52261:CDS	50.0%
	TGCATCCACTCGAAGGAAAG+CGG	+	chr7.4:28488540-28488559	None:intergenic	50.0%
	TTCGAGAGGTGGCAGACTAA+TGG	+	chr7.4:28487884-28487903	None:intergenic	50.0%
	TTGGAACTGAATGCCGAGAG+TGG	-	chr7.4:28488318-28488337	MS.gene52261:CDS	50.0%
!	TTTTGGGCTGTAGCCATGGT+TGG	-	chr7.4:28487794-28487813	MS.gene52261:CDS	50.0%
!!	TAGCCATGGTTGGCTTGCAT+TGG	-	chr7.4:28487804-28487823	MS.gene52261:CDS	50.0%
	AGAGGTGGCAGACTAATGGG+AGG	+	chr7.4:28487880-28487899	None:intergenic	55.0%
	CCACCAATGCAAGCCAACCA+TGG	+	chr7.4:28487810-28487829	None:intergenic	55.0%
	TGTCATGGGGTCAGCAGACA+AGG	+	chr7.4:28487923-28487942	None:intergenic	55.0%
!!	CCATGGTTGGCTTGCATTGG+TGG	-	chr7.4:28487807-28487826	MS.gene52261:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	28487784	28488596	28487784	ID=MS.gene52261
chr7.4	mRNA	28487784	28488596	28487784	ID=MS.gene52261.t1;Parent=MS.gene52261
chr7.4	exon	28487784	28488596	28487784	ID=MS.gene52261.t1.exon1;Parent=MS.gene52261.t1
chr7.4	CDS	28487784	28488596	28487784	ID=cds.MS.gene52261.t1;Parent=MS.gene52261.t1

Gene Sequence

>MS.gene52261

ATGAAATTGTTTTTGGGCTGTAGCCATGGTTGGCTTGCATTGGTGGATGATAACGATGTCATTACTCTAGTGAATCCTTTTAAGTGTAGTATTCCTCCCATTAGTCTGCCACCTCTCGAATCTCTCAAAAAGGTTACCTTGTCTGCTGACCCCATGACAAATCCAAGTGATTATGTGGTTGCAGCAATTCACAAGTTTGTTTATCTCGCTTTCAAAAGAGCAAGCCAATCATTTTGGATTCGTGTAGACACCAATGAATTTTTTTTCACTAATGTTGTATTTTATAAAGGCTTGGTATTTGCTGATAGCAACAGAGACACCATTGTATCCTTAAAGCTAAACAATCCTCCTTCTGATGACTCAATTGATCGTAATTTTTCCTACTATGAGAAGATGGCTAGTACTCCATATATGATTCCAAAACTACGCTGTTATGATGGACCTGCTTATTTTGTGAAATCATTGACCGGAGACATATGGTTGATGAGAAGATGCTTAATCGACGGGTTTTCTATCAAAATGCACGTTTTCAAATTGGAACTGAATGCCGAGAGTGGAAAACTTGAACAAATGAACAAACTTGAGAGTTTGGAAGACAACATTTTATTTGTCGGAAGTGTTGGTGATTCAATTTCTGTGTCAGCCTCTTGTTTCTCCACATTGGAAAAGGATTCGATTTATATGAATGATCATATGGATTCAGGGTTAAGAATCTATAATGTGAAGGATGGAAGTTATCGCAATCAATTCTTACCGCTTTCCTTCGAGTGGATGCATCATTTCTGGGTTCTGCCACAATGTCAATGGGATTGA

Protein sequence

>MS.gene52261.t1

MKLFLGCSHGWLALVDDNDVITLVNPFKCSIPPISLPPLESLKKVTLSADPMTNPSDYVVAAIHKFVYLAFKRASQSFWIRVDTNEFFFTNVVFYKGLVFADSNRDTIVSLKLNNPPSDDSIDRNFSYYEKMASTPYMIPKLRCYDGPAYFVKSLTGDIWLMRRCLIDGFSIKMHVFKLELNAESGKLEQMNKLESLEDNILFVGSVGDSISVSASCFSTLEKDSIYMNDHMDSGLRIYNVKDGSYRNQFLPLSFEWMHHFWVLPQCQWD