AlfalfaGEDB | MS.gene29654

M. sativa cultivar XinJiangDaYe / MS.gene29654

KEGG: -
GO: -
PFAM: PF03478.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29654.t1	RHN57146.1	86.3	256	28	1	7	262	6	254	9.10E-123	449.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29654.t1	A0A396HUX2	86.3	256	28	1	7	262	6	254	6.5e-123	449.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049853	MS.gene29654	0.806049	9.93E-50	-1.69E-46
MS.gene052447	MS.gene29654	0.895183	1.22E-75	-1.69E-46
MS.gene05329	MS.gene29654	0.813087	3.06E-51	-1.69E-46
MS.gene054174	MS.gene29654	0.816345	5.80E-52	-1.69E-46
MS.gene054175	MS.gene29654	0.80528	1.44E-49	-1.69E-46
MS.gene055952	MS.gene29654	0.80521	1.49E-49	-1.69E-46
MS.gene059458	MS.gene29654	0.824675	7.10E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene022086	MS.gene29654	PPI
MS.gene023785	MS.gene29654	PPI
MS.gene023787	MS.gene29654	PPI
MS.gene023889	MS.gene29654	PPI
MS.gene023891	MS.gene29654	PPI
MS.gene023893	MS.gene29654	PPI
MS.gene027042	MS.gene29654	PPI
MS.gene029514	MS.gene29654	PPI
MS.gene032780	MS.gene29654	PPI
MS.gene032784	MS.gene29654	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29654.t1	MTR_5g082780	90.909	231	21	0	33	263	1	231	5.03e-155	432
MS.gene29654.t1	MTR_5g082770	58.173	208	74	5	21	219	87	290	2.71e-68	214
MS.gene29654.t1	MTR_1g064570	40.248	323	144	10	10	294	87	398	4.27e-67	214
MS.gene29654.t1	MTR_5g038260	43.403	288	140	8	26	294	98	381	5.13e-64	206
MS.gene29654.t1	MTR_5g038230	43.369	279	127	8	26	294	96	353	4.29e-60	195
MS.gene29654.t1	MTR_1g108740	38.255	298	138	9	10	294	80	344	9.96e-51	171
MS.gene29654.t1	MTR_7g077620	40.753	292	122	12	26	294	107	370	1.63e-46	160
MS.gene29654.t1	MTR_7g077660	40.273	293	120	15	26	294	105	366	7.75e-45	156
MS.gene29654.t1	MTR_7g077650	39.041	292	126	14	26	293	106	369	1.66e-43	152
MS.gene29654.t1	MTR_7g076100	40.081	247	110	10	68	294	70	298	5.21e-38	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 60 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTTGGTAAAACCCAGAA+AGG	0.548520	5.1:+64869950	None:intergenic
AACCATGCTGGACTTATGTA+TGG	0.478737	5.1:-64870498	MS.gene29654:CDS
AACTTGTATATGATCGATCG+AGG	0.611957	5.1:+64870641	None:intergenic
AAGTTAGATTGTGTAACTTG+TGG	0.613074	5.1:-64870064	MS.gene29654:CDS
AATCATTAGAGGGAGACTTG+TGG	0.640892	5.1:-64870306	MS.gene29654:CDS
AATTAATCTCATTGGAAATT+TGG	0.118149	5.1:+64869935	None:intergenic
ACCAAATGATTATATGGTTG+CGG	0.497077	5.1:-64870569	MS.gene29654:CDS
ACGAGAGTTGCAGCCACCAT+TGG	0.479084	5.1:+64871538	None:intergenic
ACTTGTATATGATCGATCGA+GGG	0.674359	5.1:+64870642	None:intergenic
ACTTGTGGTCCATTTCATAT+AGG	0.341950	5.1:-64870049	MS.gene29654:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTTGGTAAAACCCAGAA+AGG	+	chr5.1:64871531-64871550	None:intergenic	30.0%
AAGTTAGATTGTGTAACTTG+TGG	-	chr5.1:64871414-64871433	MS.gene29654:intron	30.0%
ACAAACATCTTTCTGTTCTT+GGG	+	chr5.1:64870657-64870676	None:intergenic	30.0%
ACCAAATGATTATATGGTTG+CGG	-	chr5.1:64870909-64870928	MS.gene29654:intron	30.0%
AGAAGGATGCAATATTGTTA+TGG	+	chr5.1:64870788-64870807	None:intergenic	30.0%
GGGATATATAATGTAAGAGA+TGG	-	chr5.1:64871450-64871469	MS.gene29654:intron	30.0%
GGTTAGAATTGAACTAGATT+GGG	-	chr5.1:64870471-64870490	MS.gene29654:CDS	30.0%
TACAAACATCTTTCTGTTCT+TGG	+	chr5.1:64870658-64870677	None:intergenic	30.0%
TATGTTGCGAGTAAATATCA+CGG	-	chr5.1:64871057-64871076	MS.gene29654:intron	30.0%
TGGTTAGAATTGAACTAGAT+TGG	-	chr5.1:64870470-64870489	MS.gene29654:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	64869943	64871557	64869943	ID=MS.gene29654
chr5.1	mRNA	64869943	64871557	64869943	ID=MS.gene29654.t1;Parent=MS.gene29654
chr5.1	exon	64871481	64871557	64871481	ID=MS.gene29654.t1.exon1;Parent=MS.gene29654.t1
chr5.1	CDS	64871481	64871557	64871481	ID=cds.MS.gene29654.t1;Parent=MS.gene29654.t1
chr5.1	exon	64869943	64870750	64869943	ID=MS.gene29654.t1.exon2;Parent=MS.gene29654.t1
chr5.1	CDS	64869943	64870750	64869943	ID=cds.MS.gene29654.t1;Parent=MS.gene29654.t1

Gene Sequence

>MS.gene29654

ATGGTGGCTGCAACTCTCGTTCCCAACCCGCACAAACGGCAGCTAATTGGGGCACTTAGCAGCACAACAACAGAGAGGTATATGTAAATCTTTTTTTTTTGTCTGTAATGGATGAATAGTTTCCACTATGGAAAATTGCTTTTTTTTTTTGTTCTTTTTTTTTTAAATTGGAAGTTCTCCACACTCAATTGCGATAATCCTCGAGCCTCTTGAAATCCGAATGAGAGAAAAGTTTTCTGTCTCAATTTGTGGTTGATATTTGAAAATTCTAATTGATAATTAGTACAACAATTAATATTAATTATTATTGTAGGATTGCTAGGTACGCTCTCTAACACACACATAGTACATCCCAACACATACTCTTCTATTTGTTGAATGTCACACATGAGTTCCACCAAAATTATGTGAGACATAATTTGATGGGAGCCATATGAATTTCAACAAACAGAGGAAAGTGTGTTCCTAACACTTCTAAATTGGATATATATGCTTATATTATCTTATATAGTTCCTTCTTGTTATATTGGTTAGAATTGAACTAGATTGGGCAAATTTGGAAGCACTTGCACTTGAATTAATTCTTGATAAGTTGGAAGAACAAATAGATCACATTTGTTTTGGTGCCGTGTGCAAGAGTTGGCAATTAATTGCAAAGCTTAACCATCAAAATCAGCGGTTCAGAAGTCATGTACTACCCATGCTAATGATCCCAAGAACAGAAAGATGTTTGTATAGCATTTCAGCCAATAAAATGTATCCATTTAAATTGCCAAATTCTTATTACCATATTTGTTGTGGATTTAGCCATGGTTGGCTTGCAACAATTGATAAAAGTCATGCCATAACAATATTGCATCCTTCTAAGAATGTTGCTCCTATTAGTCTTCCAACCCTCGATCGATCATATACAAGTTTATCTGAGGTCGGGTACAAGGTCACTCTTTCTGCTGACCCTATAAAAAGACCAAATGATTATATGGTTGCGGCAATTCACACTAGCATTCATGGACGTTTCGATTTTATAAAAGCGGGCGAACCATGCTGGACTTATGTATGGATCCCGAGCTGGCATCATTCCTTTATGGATGTTATGTTCTATGGAGGTTTAGTTTATGTTGCGAGTAAATATCACGGTATAGTATCTTTTAATCTTGGTCATAAGAGGATAACTCCAAATCCATTTCAGGACATAGCTTTTCATGGAAGGTTTAAAGGTTATCTTGTGAAATCATTAGAGGGAGACTTGTGGATGGTGAAAAGATTTTTTACTTTTAAGATTGATTTCCAAGTGTACAAGTTTGAATTCGATGCTCAGACCGGAAAGTTTAAACAAATGCTAAAGCTTGAGAGTCTTGGAGACAATGTTTTATTCTTGGGAGACACTGATTCAATTTCTGTATCAGCTTCATACTTCTCCGGTTGTCTGAAAAAAGATTCAATTTATTACACAGAAAGTATTAATGCAAACAAGTTAGATTGTGTAACTTGTGGTCCATTTCATATAGGGATATATAATGTAAGAGATGGAACCTCTGATCATCATAATCTTCACAATCATTTCTTCAACAAGCAGCAGCCACCACCTTTCTGGGTTTTACCAAATTTCCAATGA

Protein sequence

>MS.gene29654.t1

MVAATLVPNPHKRQLIGALSSTTTESHGWLATIDKSHAITILHPSKNVAPISLPTLDRSYTSLSEVGYKVTLSADPIKRPNDYMVAAIHTSIHGRFDFIKAGEPCWTYVWIPSWHHSFMDVMFYGGLVYVASKYHGIVSFNLGHKRITPNPFQDIAFHGRFKGYLVKSLEGDLWMVKRFFTFKIDFQVYKFEFDAQTGKFKQMLKLESLGDNVLFLGDTDSISVSASYFSGCLKKDSIYYTESINANKLDCVTCGPFHIGIYNVRDGTSDHHNLHNHFFNKQQPPPFWVLPNFQ