AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52913

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52913.t1 MTR_7g077620 67.655 371 103 4 1 355 1 370 1.08e-168 476
MS.gene52913.t1 MTR_7g077650 64.021 378 109 10 1 357 1 372 6.53e-151 430
MS.gene52913.t1 MTR_7g077660 60.638 376 121 11 1 357 1 368 4.48e-138 397
MS.gene52913.t1 MTR_7g076110 49.492 295 128 6 81 357 22 313 7.74e-82 252
MS.gene52913.t1 MTR_1g108740 44.054 370 166 16 1 355 1 344 2.87e-81 251
MS.gene52913.t1 MTR_7g076100 58.051 236 87 3 133 357 66 300 1.30e-80 248
MS.gene52913.t1 MTR_7g076100 74.000 50 10 2 1 47 1 50 5.53e-15 75.1
MS.gene52913.t1 MTR_5g038260 40.100 399 176 16 3 357 4 383 2.97e-70 224
MS.gene52913.t1 MTR_1g064570 39.474 418 171 17 3 357 2 400 9.20e-69 221
MS.gene52913.t1 MTR_5g038230 39.793 387 160 15 5 355 4 353 1.67e-66 214
MS.gene52913.t1 MTR_5g082770 45.066 304 130 10 5 290 4 288 2.42e-63 204
MS.gene52913.t1 MTR_1g016150 47.980 198 92 7 1 190 1 195 5.97e-48 162
MS.gene52913.t1 MTR_5g082780 40.789 228 111 8 109 322 1 218 2.22e-34 126
MS.gene52913.t1 MTR_7g076130 50.000 130 50 5 163 278 25 153 3.62e-26 102
MS.gene52913.t1 MTR_1g108750 46.358 151 50 6 5 154 4 124 6.16e-23 92.8
MS.gene52913.t1 MTR_3g114990 27.225 382 200 17 4 334 22 376 1.01e-18 87.0
MS.gene52913.t1 MTR_3g114970 23.631 347 202 12 4 318 3 318 8.74e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTCTACGTTTACAAAT+TGG 0.205980 6.1:-21956474 MS.gene52913:CDS
AACTTCCATCCTTCGCATTA+TGG 0.220578 6.1:+21956270 None:intergenic
TTTCAACATACTTGAGAGTT+TGG 0.271304 6.1:-21956420 MS.gene52913:CDS
CAACCGGATGTCACATCTTT+GGG 0.283463 6.1:-21956219 MS.gene52913:CDS
TAATTACTCGGGCTTGTAAT+CGG 0.293843 6.1:+21956812 None:intergenic
GTTGAACCCGAAGCTTAAAA+TGG 0.296182 6.1:+21956643 None:intergenic
TCAACCGGATGTCACATCTT+TGG 0.300744 6.1:-21956220 MS.gene52913:CDS
TTTCCAGAACAATATCATTA+TGG 0.309110 6.1:-21956581 MS.gene52913:CDS
GGAGCTACACTATACTTAAA+AGG 0.314471 6.1:+21956887 None:intergenic
GACATCCGGTTGAAGGAAAT+TGG 0.322776 6.1:+21956230 None:intergenic
TGGATTCGTGTAAACAGATA+TGG 0.324446 6.1:-21956728 MS.gene52913:CDS
CAATGAAATGACGGTGTGTT+TGG 0.335412 6.1:-21956969 MS.gene52913:CDS
AAGAACACATAGATCATGTT+AGG 0.348741 6.1:-21957180 MS.gene52913:CDS
GAAGGATGAGATAATTGAAT+AGG 0.358837 6.1:+21956995 None:intergenic
AAAAGGATTCGCTAGTGTTA+TGG 0.360726 6.1:+21956904 None:intergenic
ACACACCGTCATTTCATTGA+TGG 0.365745 6.1:+21956973 None:intergenic
GGCAATGAGGAAGGTAGATT+GGG 0.372533 6.1:-21957248 None:intergenic
AGAAAGTCTACCTCTTAGTT+TGG 0.381080 6.1:-21957218 MS.gene52913:CDS
AGGCAATGAGGAAGGTAGAT+TGG 0.382674 6.1:-21957249 None:intergenic
CTACGTTTACAAATTGGAAC+TGG 0.389715 6.1:-21956468 MS.gene52913:CDS
CTTTACCAATTTCCTTCAAC+CGG 0.393860 6.1:-21956235 MS.gene52913:CDS
CCATGGTTGGCTTGCATTGG+TGG 0.402911 6.1:-21956939 MS.gene52913:CDS
TTGGAGAGGTGGCAGACTAA+TGG 0.403671 6.1:+21956865 None:intergenic
GGGTTCTACCACAATTTCAA+TGG 0.406178 6.1:-21956199 MS.gene52913:CDS
CCTCCATAATGATATTGTTC+TGG 0.422685 6.1:+21956578 None:intergenic
AAAGAGCAAGTCAATCATCT+TGG 0.426179 6.1:-21956748 MS.gene52913:CDS
AATTACTCGGGCTTGTAATC+GGG 0.426509 6.1:+21956813 None:intergenic
TGGAGAGGTGGCAGACTAAT+GGG 0.429349 6.1:+21956866 None:intergenic
AGTACTAGCCATCTCATAAC+AGG 0.432398 6.1:+21956613 None:intergenic
AAGGATGAGATAATTGAATA+GGG 0.458975 6.1:+21956996 None:intergenic
TTGTTTCTCCAAGTTTGAAA+AGG 0.460734 6.1:-21956345 MS.gene52913:CDS
AATGAAATGACGGTGTGTTT+GGG 0.468609 6.1:-21956968 MS.gene52913:CDS
TAGTTTGGTTCTTGAGAAGT+TGG 0.473111 6.1:-21957203 MS.gene52913:CDS
GGTTCTACCACAATTTCAAT+GGG 0.480142 6.1:-21956198 MS.gene52913:CDS
TAGCCATGGTTGGCTTGCAT+TGG 0.484269 6.1:-21956942 MS.gene52913:CDS
ACCTCTCCAATCTGTTTACA+AGG 0.490029 6.1:-21956852 MS.gene52913:CDS
TTCTCAAGAACCAAACTAAG+AGG 0.491952 6.1:+21957208 None:intergenic
CTGATATTTGCTGTTGGCAA+TGG 0.502091 6.1:-21956671 MS.gene52913:CDS
AAGATGTGACATCCGGTTGA+AGG 0.515408 6.1:+21956223 None:intergenic
CAATATCATTATGGAGGGCA+TGG 0.528364 6.1:-21956572 MS.gene52913:CDS
GATCAATCCCATTGAAATTG+TGG 0.531759 6.1:+21956191 None:intergenic
AATCATTGACCGGAGACCTA+TGG 0.532480 6.1:-21956538 MS.gene52913:CDS
CCAGAACAATATCATTATGG+AGG 0.533728 6.1:-21956578 MS.gene52913:CDS
GTGTGTTTGGGCGTTAGCCA+TGG 0.536708 6.1:-21956956 MS.gene52913:CDS
TTTCACCATCCATAGGTCTC+CGG 0.537035 6.1:+21956529 None:intergenic
CAAATCCATAATGCGAAGGA+TGG 0.537880 6.1:-21956275 MS.gene52913:CDS
AAAGGTCTGATATTTGCTGT+TGG 0.547450 6.1:-21956677 MS.gene52913:CDS
GTTTGGGCGTTAGCCATGGT+TGG 0.554659 6.1:-21956952 MS.gene52913:CDS
AGGTAACCTTGTAAACAGAT+TGG 0.555212 6.1:+21956846 None:intergenic
GAACCCAAAGATGTGACATC+CGG 0.561035 6.1:+21956216 None:intergenic
TTCGTGTGGTGTGCAAGACT+TGG 0.561436 6.1:-21957156 MS.gene52913:CDS
CAGAACAATATCATTATGGA+GGG 0.569165 6.1:-21956577 MS.gene52913:CDS
CAATTCTGCCTGTTATGAGA+TGG 0.570742 6.1:-21956621 MS.gene52913:CDS
GTTGCAGCGATTTACAATTG+TGG 0.573110 6.1:-21956785 MS.gene52913:CDS
TTCAAACTTGGAGAAACAAG+AGG 0.574529 6.1:+21956349 None:intergenic
GCTGCAACCACGTAATTACT+CGG 0.580071 6.1:+21956800 None:intergenic
AGAGGATCATCAACATAGGA+TGG 0.580233 6.1:+21957098 None:intergenic
TCCTTCCATCAATGAAATGA+CGG 0.584673 6.1:-21956978 MS.gene52913:CDS
TATGAGAATTATTGTATGAG+AGG 0.586159 6.1:+21957080 None:intergenic
ACCGTCATTTCATTGATGGA+AGG 0.592100 6.1:+21956977 None:intergenic
ATTATGATAGGCAATGAGGA+AGG 0.609073 6.1:-21957257 None:intergenic
TTGTAAACAGATTGGAGAGG+TGG 0.610040 6.1:+21956854 None:intergenic
AACTTGGAGAAACAAGAGGC+TGG 0.613015 6.1:+21956353 None:intergenic
TTGGAACTGGACGCTATGAG+TGG 0.614160 6.1:-21956455 MS.gene52913:CDS
GCAATGAGGAAGGTAGATTG+GGG 0.614437 6.1:-21957247 MS.gene52913:CDS
TATGAGAGGATCATCAACAT+AGG 0.615056 6.1:+21957094 None:intergenic
GTTACAAATCCATAATGCGA+AGG 0.615721 6.1:-21956279 MS.gene52913:CDS
CTGCAACCACGTAATTACTC+GGG 0.626596 6.1:+21956801 None:intergenic
AGTTTGCATAAATGCTTGCG+AGG 0.629904 6.1:+21956491 None:intergenic
AGATCATGTTAGGTTTCGTG+TGG 0.631367 6.1:-21957170 MS.gene52913:CDS
AAATTGGTAAAGATTGACTA+CGG 0.637052 6.1:+21956246 None:intergenic
TGTAATCGGGTCATCAGACA+AGG 0.645501 6.1:+21956826 None:intergenic
CCACCAATGCAAGCCAACCA+TGG 0.665097 6.1:+21956939 None:intergenic
TACAAGCCCGAGTAATTACG+TGG 0.694443 6.1:-21956807 MS.gene52913:CDS
ATTGACCGGAGACCTATGGA+TGG 0.696827 6.1:-21956534 MS.gene52913:CDS
ACCTTGTAAACAGATTGGAG+AGG 0.742777 6.1:+21956851 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTATTTTATTTATGA+TGG - chr6.1:21957117-21957136 MS.gene52913:CDS 10.0%
!!! AACATTTTATTTGTTGGAAT+CGG - chr6.1:21957045-21957064 MS.gene52913:CDS 20.0%
!!! ACTGATGTTGTATTTTATAA+AGG - chr6.1:21956742-21956761 MS.gene52913:CDS 20.0%
!!! GAAAACAACATTTTATTTGT+TGG - chr6.1:21957039-21957058 MS.gene52913:CDS 20.0%
! AAATTGGTAAAGATTGACTA+CGG + chr6.1:21957194-21957213 None:intergenic 25.0%
! AAGGATGAGATAATTGAATA+GGG + chr6.1:21956444-21956463 None:intergenic 25.0%
! TATGAGAATTATTGTATGAG+AGG + chr6.1:21956360-21956379 None:intergenic 25.0%
! TTTCCAGAACAATATCATTA+TGG - chr6.1:21956856-21956875 MS.gene52913:CDS 25.0%
!! AATTGAATCCTTTTCAAACT+TGG + chr6.1:21957103-21957122 None:intergenic 25.0%
!! TATTTTGTGAAATCATTGAC+CGG - chr6.1:21956889-21956908 MS.gene52913:CDS 25.0%
!!! TATTTTATTTATGATGGCGA+CGG - chr6.1:21957123-21957142 MS.gene52913:CDS 25.0%
AAGAACACATAGATCATGTT+AGG - chr6.1:21956257-21956276 MS.gene52913:CDS 30.0%
CAAACTCTACGTTTACAAAT+TGG - chr6.1:21956963-21956982 MS.gene52913:CDS 30.0%
CAGAACAATATCATTATGGA+GGG - chr6.1:21956860-21956879 MS.gene52913:CDS 30.0%
GAAGGATGAGATAATTGAAT+AGG + chr6.1:21956445-21956464 None:intergenic 30.0%
TTTCAACATACTTGAGAGTT+TGG - chr6.1:21957017-21957036 MS.gene52913:CDS 30.0%
! ACATAGGATGGACATTTTTA+TGG + chr6.1:21956330-21956349 None:intergenic 30.0%
! TTGTTTCTCCAAGTTTGAAA+AGG - chr6.1:21957092-21957111 MS.gene52913:CDS 30.0%
AAAAGGATTCGCTAGTGTTA+TGG + chr6.1:21956536-21956555 None:intergenic 35.0%
AAAGAGCAAGTCAATCATCT+TGG - chr6.1:21956689-21956708 MS.gene52913:CDS 35.0%
AATGAAATGACGGTGTGTTT+GGG - chr6.1:21956469-21956488 MS.gene52913:CDS 35.0%
AGAAAGTCTACCTCTTAGTT+TGG - chr6.1:21956219-21956238 MS.gene52913:CDS 35.0%
AGGTAACCTTGTAAACAGAT+TGG + chr6.1:21956594-21956613 None:intergenic 35.0%
CCAGAACAATATCATTATGG+AGG - chr6.1:21956859-21956878 MS.gene52913:CDS 35.0%
CCTCCATAATGATATTGTTC+TGG + chr6.1:21956862-21956881 None:intergenic 35.0%
CTACGTTTACAAATTGGAAC+TGG - chr6.1:21956969-21956988 MS.gene52913:CDS 35.0%
CTTTACCAATTTCCTTCAAC+CGG - chr6.1:21957202-21957221 MS.gene52913:CDS 35.0%
GGAGCTACACTATACTTAAA+AGG + chr6.1:21956553-21956572 None:intergenic 35.0%
GGTTCTACCACAATTTCAAT+GGG - chr6.1:21957239-21957258 MS.gene52913:CDS 35.0%
GTTACAAATCCATAATGCGA+AGG - chr6.1:21957158-21957177 MS.gene52913:CDS 35.0%
TAATTACTCGGGCTTGTAAT+CGG + chr6.1:21956628-21956647 None:intergenic 35.0%
TATGAGAGGATCATCAACAT+AGG + chr6.1:21956346-21956365 None:intergenic 35.0%
TCCTTCCATCAATGAAATGA+CGG - chr6.1:21956459-21956478 MS.gene52913:CDS 35.0%
TGGATTCGTGTAAACAGATA+TGG - chr6.1:21956709-21956728 MS.gene52913:CDS 35.0%
TTCAAACTTGGAGAAACAAG+AGG + chr6.1:21957091-21957110 None:intergenic 35.0%
TTCTCAAGAACCAAACTAAG+AGG + chr6.1:21956232-21956251 None:intergenic 35.0%
! AACATCTTTTCACCATCCAT+AGG + chr6.1:21956918-21956937 None:intergenic 35.0%
!! AAAGGTCTGATATTTGCTGT+TGG - chr6.1:21956760-21956779 MS.gene52913:CDS 35.0%
!! TAGTTTGGTTCTTGAGAAGT+TGG - chr6.1:21956234-21956253 MS.gene52913:CDS 35.0%
AACTTCCATCCTTCGCATTA+TGG + chr6.1:21957170-21957189 None:intergenic 40.0%
AATTACTCGGGCTTGTAATC+GGG + chr6.1:21956627-21956646 None:intergenic 40.0%
ACACACCGTCATTTCATTGA+TGG + chr6.1:21956467-21956486 None:intergenic 40.0%
ACCGTCATTTCATTGATGGA+AGG + chr6.1:21956463-21956482 None:intergenic 40.0%
ACCTCTCCAATCTGTTTACA+AGG - chr6.1:21956585-21956604 MS.gene52913:CDS 40.0%
ACCTTGTAAACAGATTGGAG+AGG + chr6.1:21956589-21956608 None:intergenic 40.0%
AGAGGATCATCAACATAGGA+TGG + chr6.1:21956342-21956361 None:intergenic 40.0%
AGATCATGTTAGGTTTCGTG+TGG - chr6.1:21956267-21956286 MS.gene52913:CDS 40.0%
AGTACTAGCCATCTCATAAC+AGG + chr6.1:21956827-21956846 None:intergenic 40.0%
AGTTTGCATAAATGCTTGCG+AGG + chr6.1:21956949-21956968 None:intergenic 40.0%
CAAATCCATAATGCGAAGGA+TGG - chr6.1:21957162-21957181 MS.gene52913:CDS 40.0%
CAATATCATTATGGAGGGCA+TGG - chr6.1:21956865-21956884 MS.gene52913:CDS 40.0%
CAATGAAATGACGGTGTGTT+TGG - chr6.1:21956468-21956487 MS.gene52913:CDS 40.0%
CAATTCTGCCTGTTATGAGA+TGG - chr6.1:21956816-21956835 MS.gene52913:CDS 40.0%
GGGTTCTACCACAATTTCAA+TGG - chr6.1:21957238-21957257 MS.gene52913:CDS 40.0%
GTTGAACCCGAAGCTTAAAA+TGG + chr6.1:21956797-21956816 None:intergenic 40.0%
GTTGCAGCGATTTACAATTG+TGG - chr6.1:21956652-21956671 MS.gene52913:CDS 40.0%
TTGTAAACAGATTGGAGAGG+TGG + chr6.1:21956586-21956605 None:intergenic 40.0%
! GAGACACCATTTTAAGCTTC+GGG - chr6.1:21956788-21956807 MS.gene52913:CDS 40.0%
! GGAGACACCATTTTAAGCTT+CGG - chr6.1:21956787-21956806 MS.gene52913:CDS 40.0%
!! CTGATATTTGCTGTTGGCAA+TGG - chr6.1:21956766-21956785 MS.gene52913:CDS 40.0%
AACTTGGAGAAACAAGAGGC+TGG + chr6.1:21957087-21957106 None:intergenic 45.0%
AAGATGTGACATCCGGTTGA+AGG + chr6.1:21957217-21957236 None:intergenic 45.0%
AATCATTGACCGGAGACCTA+TGG - chr6.1:21956899-21956918 MS.gene52913:CDS 45.0%
CAACCGGATGTCACATCTTT+GGG - chr6.1:21957218-21957237 MS.gene52913:CDS 45.0%
CTGCAACCACGTAATTACTC+GGG + chr6.1:21956639-21956658 None:intergenic 45.0%
GAACCCAAAGATGTGACATC+CGG + chr6.1:21957224-21957243 None:intergenic 45.0%
GACATCCGGTTGAAGGAAAT+TGG + chr6.1:21957210-21957229 None:intergenic 45.0%
GCTGCAACCACGTAATTACT+CGG + chr6.1:21956640-21956659 None:intergenic 45.0%
TACAAGCCCGAGTAATTACG+TGG - chr6.1:21956630-21956649 MS.gene52913:CDS 45.0%
TCAACCGGATGTCACATCTT+TGG - chr6.1:21957217-21957236 MS.gene52913:CDS 45.0%
TGTAATCGGGTCATCAGACA+AGG + chr6.1:21956614-21956633 None:intergenic 45.0%
TTTCACCATCCATAGGTCTC+CGG + chr6.1:21956911-21956930 None:intergenic 45.0%
ATTGACCGGAGACCTATGGA+TGG - chr6.1:21956903-21956922 MS.gene52913:CDS 50.0%
TGGAGAGGTGGCAGACTAAT+GGG + chr6.1:21956574-21956593 None:intergenic 50.0%
TTCGTGTGGTGTGCAAGACT+TGG - chr6.1:21956281-21956300 MS.gene52913:CDS 50.0%
TTGGAACTGGACGCTATGAG+TGG - chr6.1:21956982-21957001 MS.gene52913:CDS 50.0%
TTGGAGAGGTGGCAGACTAA+TGG + chr6.1:21956575-21956594 None:intergenic 50.0%
!! TAGCCATGGTTGGCTTGCAT+TGG - chr6.1:21956495-21956514 MS.gene52913:CDS 50.0%
CCACCAATGCAAGCCAACCA+TGG + chr6.1:21956501-21956520 None:intergenic 55.0%
GTGTGTTTGGGCGTTAGCCA+TGG - chr6.1:21956481-21956500 MS.gene52913:CDS 55.0%
GTTTGGGCGTTAGCCATGGT+TGG - chr6.1:21956485-21956504 MS.gene52913:CDS 55.0%
!! CCATGGTTGGCTTGCATTGG+TGG - chr6.1:21956498-21956517 MS.gene52913:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 21956193 21957266 21956193 ID=MS.gene52913
chr6.1 mRNA 21956193 21957266 21956193 ID=MS.gene52913.t1;Parent=MS.gene52913
chr6.1 exon 21956193 21957266 21956193 ID=MS.gene52913.t1.exon1;Parent=MS.gene52913.t1
chr6.1 CDS 21956193 21957266 21956193 ID=cds.MS.gene52913.t1;Parent=MS.gene52913.t1
Gene Sequence

>MS.gene52913

ATGAGGAAGGTAGATTGGGGAAATTTAGAAAGTCTACCTCTTAGTTTGGTTCTTGAGAAGTTGGAAGAACACATAGATCATGTTAGGTTTCGTGTGGTGTGCAAGACTTGGCTATTAGTTGCAAATCATCTTAACCATAAAAATGTCCATCCTATGTTGATGATCCTCTCATACAATAATTCTCATATACGTACAATATCACAGACGAAGAAAAGATCTTTGTATAGTATTCTTAGTGAGAAAGAGTACCCTATTCAATTATCTCATCCTTCCATCAATGAAATGACGGTGTGTTTGGGCGTTAGCCATGGTTGGCTTGCATTGGTGGATGACAACAAAGCCATAACACTAGCGAATCCTTTTAAGTATAGTGTAGCTCCCATTAGTCTGCCACCTCTCCAATCTGTTTACAAGGTTACCTTGTCTGATGACCCGATTACAAGCCCGAGTAATTACGTGGTTGCAGCGATTTACAATTGTGGTTCTCTCGCTTTCAAAAGAGCAAGTCAATCATCTTGGATTCGTGTAAACAGATATGGATTTTATGTCACTGATGTTGTATTTTATAAAGGTCTGATATTTGCTGTTGGCAATGGAGACACCATTTTAAGCTTCGGGTTCAACAATTCTGCCTGTTATGAGATGGCTAGTACTCAGAATTTTTTTCCAGAACAATATCATTATGGAGGGCATGGTTATTTTGTGAAATCATTGACCGGAGACCTATGGATGGTGAAAAGATGTTTAATTGATCCTCGCAAGCATTTATGCAAACTCTACGTTTACAAATTGGAACTGGACGCTATGAGTGGAAAAGTTAAACATTTCAACATACTTGAGAGTTTGGAAAACAACATTTTATTTGTTGGAATCGGCGATTCAATTTCTGTGCCAGCCTCTTGTTTCTCCAAGTTTGAAAAGGATTCAATTTATTTTATTTATGATGGCGACGGAGATGATTTAGAGTTACAAATCCATAATGCGAAGGATGGAAGTTTCCGTAGTCAATCTTTACCAATTTCCTTCAACCGGATGTCACATCTTTGGGTTCTACCACAATTTCAATGGGATTGA
Protein sequence

>MS.gene52913.t1

MRKVDWGNLESLPLSLVLEKLEEHIDHVRFRVVCKTWLLVANHLNHKNVHPMLMILSYNNSHIRTISQTKKRSLYSILSEKEYPIQLSHPSINEMTVCLGVSHGWLALVDDNKAITLANPFKYSVAPISLPPLQSVYKVTLSDDPITSPSNYVVAAIYNCGSLAFKRASQSSWIRVNRYGFYVTDVVFYKGLIFAVGNGDTILSFGFNNSACYEMASTQNFFPEQYHYGGHGYFVKSLTGDLWMVKRCLIDPRKHLCKLYVYKLELDAMSGKVKHFNILESLENNILFVGIGDSISVPASCFSKFEKDSIYFIYDGDGDDLELQIHNAKDGSFRSQSLPISFNRMSHLWVLPQFQWD