AlfalfaGEDB | MS.gene40233

M. sativa cultivar XinJiangDaYe / MS.gene40233

KEGG: K19035
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019843 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0097159 GO:0110165 GO:1901363 GO:1901564 GO:1901566 GO:1901576
PFAM: PF17257.5

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40233.t1	XP_003603663.1	94.2	104	6	0	1	104	1	104	3.40E-40	174.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40233.t1	P11892	85.4	89	12	1	1	88	1	89	9.7e-36	150.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40233.t1	B7FHD4	94.2	104	6	0	1	104	1	104	2.5e-40	174.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049284	MS.gene40233	0.802376	5.78E-49	-1.69E-46
MS.gene049351	MS.gene40233	0.820884	5.42E-53	-1.69E-46
MS.gene049389	MS.gene40233	0.829051	6.39E-55	-1.69E-46
MS.gene049427	MS.gene40233	0.829977	3.81E-55	-1.69E-46
MS.gene049491	MS.gene40233	0.865698	4.89E-65	-1.69E-46
MS.gene049614	MS.gene40233	0.848763	4.98E-60	-1.69E-46
MS.gene049661	MS.gene40233	0.848147	7.38E-60	-1.69E-46
MS.gene049662	MS.gene40233	0.846735	1.80E-59	-1.69E-46
MS.gene049758	MS.gene40233	0.841041	6.04E-58	-1.69E-46
MS.gene049760	MS.gene40233	0.823642	1.24E-53	-1.69E-46
MS.gene050076	MS.gene40233	0.858788	6.47E-63	-1.69E-46
MS.gene050099	MS.gene40233	0.857956	1.15E-62	-1.69E-46
MS.gene050128	MS.gene40233	0.842146	3.09E-58	-1.69E-46
MS.gene050301	MS.gene40233	0.867641	1.18E-65	-1.69E-46
MS.gene050397	MS.gene40233	0.83234	1.00E-55	-1.69E-46
MS.gene050404	MS.gene40233	0.849796	2.57E-60	-1.69E-46
MS.gene050406	MS.gene40233	0.840292	9.49E-58	-1.69E-46
MS.gene050432	MS.gene40233	0.845892	3.06E-59	-1.69E-46
MS.gene050464	MS.gene40233	0.831851	1.32E-55	-1.69E-46
MS.gene050529	MS.gene40233	0.853737	1.96E-61	-1.69E-46
MS.gene05053	MS.gene40233	0.811482	6.85E-51	-1.69E-46
MS.gene050767	MS.gene40233	0.908115	2.43E-81	-1.69E-46
MS.gene050770	MS.gene40233	0.908932	9.94E-82	-1.69E-46
MS.gene05101	MS.gene40233	0.883598	3.92E-71	-1.69E-46
MS.gene05105	MS.gene40233	0.80808	3.69E-50	-1.69E-46
MS.gene051228	MS.gene40233	0.825455	4.64E-54	-1.69E-46
MS.gene051471	MS.gene40233	0.897639	1.16E-76	-1.69E-46
MS.gene051666	MS.gene40233	0.801632	8.22E-49	-1.69E-46
MS.gene052078	MS.gene40233	0.904898	7.58E-80	-1.69E-46
MS.gene052178	MS.gene40233	0.876393	1.45E-68	-1.69E-46
MS.gene052188	MS.gene40233	0.835408	1.71E-56	-1.69E-46
MS.gene052271	MS.gene40233	0.828664	7.93E-55	-1.69E-46
MS.gene052287	MS.gene40233	0.801399	9.17E-49	-1.69E-46
MS.gene052290	MS.gene40233	0.873115	1.89E-67	-1.69E-46
MS.gene052293	MS.gene40233	0.829205	5.87E-55	-1.69E-46
MS.gene052310	MS.gene40233	0.882988	6.56E-71	-1.69E-46
MS.gene052322	MS.gene40233	0.806995	6.27E-50	-1.69E-46
MS.gene052326	MS.gene40233	0.867674	1.15E-65	-1.69E-46
MS.gene052340	MS.gene40233	0.843288	1.54E-58	-1.69E-46
MS.gene052379	MS.gene40233	0.813448	2.55E-51	-1.69E-46
MS.gene052484	MS.gene40233	0.802763	4.81E-49	-1.69E-46
MS.gene053009	MS.gene40233	0.86295	3.52E-64	-1.69E-46
MS.gene053103	MS.gene40233	0.831739	1.41E-55	-1.69E-46
MS.gene053104	MS.gene40233	0.819509	1.12E-52	-1.69E-46
MS.gene053181	MS.gene40233	0.818561	1.84E-52	-1.69E-46
MS.gene053209	MS.gene40233	0.850785	1.36E-60	-1.69E-46
MS.gene053322	MS.gene40233	0.873069	1.96E-67	-1.69E-46
MS.gene053384	MS.gene40233	0.81174	6.02E-51	-1.69E-46
MS.gene053557	MS.gene40233	0.864747	9.73E-65	-1.69E-46
MS.gene053836	MS.gene40233	0.819135	1.36E-52	-1.69E-46
MS.gene054123	MS.gene40233	0.834709	2.56E-56	-1.69E-46
MS.gene054376	MS.gene40233	0.91156	5.30E-83	-1.69E-46
MS.gene05455	MS.gene40233	0.82191	3.14E-53	-1.69E-46
MS.gene054802	MS.gene40233	0.867712	1.12E-65	-1.69E-46
MS.gene054803	MS.gene40233	0.869122	3.92E-66	-1.69E-46
MS.gene054804	MS.gene40233	0.87202	4.38E-67	-1.69E-46
MS.gene054955	MS.gene40233	0.803028	4.24E-49	-1.69E-46
MS.gene055116	MS.gene40233	0.832713	8.08E-56	-1.69E-46
MS.gene055254	MS.gene40233	0.892387	1.66E-74	-1.69E-46
MS.gene055277	MS.gene40233	0.881784	1.80E-70	-1.69E-46
MS.gene055390	MS.gene40233	0.844415	7.67E-59	-1.69E-46
MS.gene055397	MS.gene40233	0.88552	7.57E-72	-1.69E-46
MS.gene055412	MS.gene40233	0.806603	7.59E-50	-1.69E-46
MS.gene055422	MS.gene40233	0.88547	7.91E-72	-1.69E-46
MS.gene055428	MS.gene40233	0.836305	1.01E-56	-1.69E-46
MS.gene055497	MS.gene40233	-0.83226	1.05E-55	-1.69E-46
MS.gene055499	MS.gene40233	0.860964	1.43E-63	-1.69E-46
MS.gene055647	MS.gene40233	0.808501	3.00E-50	-1.69E-46
MS.gene055712	MS.gene40233	0.803763	2.98E-49	-1.69E-46
MS.gene055896	MS.gene40233	0.861567	9.37E-64	-1.69E-46
MS.gene05592	MS.gene40233	0.837205	5.96E-57	-1.69E-46
MS.gene056191	MS.gene40233	0.834841	2.37E-56	-1.69E-46
MS.gene056407	MS.gene40233	0.808117	3.63E-50	-1.69E-46
MS.gene056410	MS.gene40233	0.834773	2.47E-56	-1.69E-46
MS.gene056609	MS.gene40233	0.879087	1.66E-69	-1.69E-46
MS.gene056612	MS.gene40233	0.84865	5.36E-60	-1.69E-46
MS.gene056796	MS.gene40233	0.804171	2.46E-49	-1.69E-46
MS.gene056897	MS.gene40233	0.812483	4.14E-51	-1.69E-46
MS.gene057011	MS.gene40233	0.813975	1.95E-51	-1.69E-46
MS.gene05702	MS.gene40233	0.842912	1.93E-58	-1.69E-46
MS.gene057123	MS.gene40233	0.838317	3.09E-57	-1.69E-46
MS.gene057129	MS.gene40233	0.873823	1.09E-67	-1.69E-46
MS.gene057295	MS.gene40233	0.887258	1.67E-72	-1.69E-46
MS.gene05733	MS.gene40233	0.827384	1.61E-54	-1.69E-46
MS.gene057439	MS.gene40233	0.842768	2.11E-58	-1.69E-46
MS.gene057448	MS.gene40233	0.811327	7.40E-51	-1.69E-46
MS.gene057449	MS.gene40233	0.818252	2.16E-52	-1.69E-46
MS.gene057450	MS.gene40233	0.8271	1.88E-54	-1.69E-46
MS.gene057796	MS.gene40233	0.809948	1.47E-50	-1.69E-46
MS.gene058002	MS.gene40233	0.832788	7.74E-56	-1.69E-46
MS.gene058082	MS.gene40233	0.847858	8.86E-60	-1.69E-46
MS.gene05811	MS.gene40233	0.825935	3.57E-54	-1.69E-46
MS.gene058233	MS.gene40233	0.824864	6.41E-54	-1.69E-46
MS.gene058276	MS.gene40233	0.809224	2.10E-50	-1.69E-46
MS.gene058293	MS.gene40233	0.894774	1.80E-75	-1.69E-46
MS.gene058365	MS.gene40233	0.817694	2.89E-52	-1.69E-46
MS.gene058660	MS.gene40233	0.889703	1.91E-73	-1.69E-46
MS.gene058787	MS.gene40233	0.831663	1.47E-55	-1.69E-46
MS.gene058845	MS.gene40233	0.812312	4.52E-51	-1.69E-46
MS.gene058903	MS.gene40233	0.808683	2.75E-50	-1.69E-46
MS.gene058928	MS.gene40233	0.832497	9.14E-56	-1.69E-46
MS.gene058993	MS.gene40233	0.812524	4.06E-51	-1.69E-46
MS.gene05907	MS.gene40233	0.823148	1.62E-53	-1.69E-46
MS.gene059228	MS.gene40233	0.827593	1.44E-54	-1.69E-46
MS.gene059294	MS.gene40233	0.80122	9.98E-49	-1.69E-46
MS.gene059326	MS.gene40233	0.900717	5.54E-78	-1.69E-46
MS.gene059342	MS.gene40233	0.816178	6.32E-52	-1.69E-46
MS.gene059518	MS.gene40233	0.844755	6.21E-59	-1.69E-46
MS.gene059536	MS.gene40233	0.847818	9.09E-60	-1.69E-46
MS.gene059597	MS.gene40233	0.856099	4.04E-62	-1.69E-46
MS.gene05959	MS.gene40233	0.814245	1.70E-51	-1.69E-46
MS.gene059676	MS.gene40233	0.866977	1.92E-65	-1.69E-46
MS.gene059688	MS.gene40233	0.861825	7.81E-64	-1.69E-46
MS.gene059692	MS.gene40233	0.892517	1.48E-74	-1.69E-46
MS.gene059695	MS.gene40233	0.88893	3.81E-73	-1.69E-46
MS.gene059708	MS.gene40233	0.824099	9.70E-54	-1.69E-46
MS.gene05984	MS.gene40233	0.820425	6.91E-53	-1.69E-46
MS.gene059858	MS.gene40233	0.81054	1.10E-50	-1.69E-46
MS.gene059894	MS.gene40233	0.878716	2.24E-69	-1.69E-46
MS.gene059960	MS.gene40233	0.888117	7.84E-73	-1.69E-46
MS.gene059974	MS.gene40233	0.847057	1.47E-59	-1.69E-46
MS.gene060005	MS.gene40233	0.851488	8.59E-61	-1.69E-46
MS.gene06037	MS.gene40233	0.852164	5.52E-61	-1.69E-46
MS.gene060407	MS.gene40233	0.80416	2.47E-49	-1.69E-46
MS.gene060800	MS.gene40233	0.803519	3.35E-49	-1.69E-46
MS.gene060804	MS.gene40233	0.818559	1.84E-52	-1.69E-46
MS.gene060818	MS.gene40233	0.844385	7.81E-59	-1.69E-46
MS.gene061041	MS.gene40233	0.817004	4.13E-52	-1.69E-46
MS.gene061049	MS.gene40233	0.868171	7.96E-66	-1.69E-46
MS.gene061051	MS.gene40233	-0.849351	3.42E-60	-1.69E-46
MS.gene061112	MS.gene40233	0.821619	3.67E-53	-1.69E-46
MS.gene061200	MS.gene40233	0.867744	1.09E-65	-1.69E-46
MS.gene061265	MS.gene40233	0.813492	2.49E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40233.t1	MTR_3g110640	97.115	104	3	0	1	104	1	104	1.85e-69	202

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40233.t1	AT5G17870	49.474	95	45	2	3	94	2	96	7.89e-23	85.1
MS.gene40233.t1	AT5G17870	49.474	95	45	2	3	94	25	119	1.89e-22	85.1

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTTTCTTTCTCAATGAATT+TGG	0.155637	3.2:-88639845	None:intergenic
TCATCGGTGTCGAGCATATT+CGG	0.324135	3.2:+88639803	MS.gene40233:CDS
TCATTGAGAAAGAAAGCAAT+TGG	0.425202	3.2:+88639851	MS.gene40233:CDS
GAAATGACAATTGTAAAATC+AGG	0.433333	3.2:-88640034	None:intergenic
CATCACTGTCGGATAGGAAT+CGG	0.473566	3.2:+88639975	MS.gene40233:CDS
AAGGTACCAAATCAAGTAGA+AGG	0.494390	3.2:-88640103	None:intergenic
AGAAAGCAATTGGAATTGAA+AGG	0.494556	3.2:+88639861	MS.gene40233:CDS
TGTCGAGCATATTCGGATGC+GGG	0.500510	3.2:+88639810	MS.gene40233:CDS
GTAGAAGGAGTAGGAGGCGG+AGG	0.503700	3.2:-88640088	None:intergenic
TGTGCAGTTGCTTTCTTCTG+TGG	0.507932	3.2:-88639926	None:intergenic
GGCAAAGGAGCATACTCTGT+TGG	0.555146	3.2:-88640001	None:intergenic
TCAGGAGGCAGTGGTGGCAA+AGG	0.562798	3.2:-88640016	None:intergenic
GTGTCGAGCATATTCGGATG+CGG	0.562802	3.2:+88639809	MS.gene40233:CDS
CCAAATCAAGTAGAAGGAGT+AGG	0.568811	3.2:-88640097	None:intergenic
ATTGTAAAATCAGGAGGCAG+TGG	0.573977	3.2:-88640025	None:intergenic
CAAGTAGAAGGAGTAGGAGG+CGG	0.578017	3.2:-88640091	None:intergenic
TGATGCATCATCAACGGCCA+CGG	0.584871	3.2:+88640060	MS.gene40233:CDS
TTCTGCTGATGCATCATCAA+CGG	0.592398	3.2:+88640054	MS.gene40233:CDS
GTAAAATCAGGAGGCAGTGG+TGG	0.601246	3.2:-88640022	None:intergenic
AATCAAGTAGAAGGAGTAGG+AGG	0.618321	3.2:-88640094	None:intergenic
ATGACAATTGTAAAATCAGG+AGG	0.620980	3.2:-88640031	None:intergenic
GAAAGGAGAAATGTATGTGG+AGG	0.621499	3.2:+88639878	MS.gene40233:CDS
GTCGAGCATATTCGGATGCG+GGG	0.622422	3.2:+88639811	MS.gene40233:CDS
ATTGAAAGGAGAAATGTATG+TGG	0.642127	3.2:+88639875	MS.gene40233:CDS
CTGCACATCACATGAAGACG+AGG	0.645185	3.2:+88639942	MS.gene40233:CDS
AGGAGGCGGAGGAACAACCG+TGG	0.742635	3.2:-88640077	None:intergenic
TGTTGATAGAGTGTTCATCG+AGG	0.792218	3.2:+88639903	MS.gene40233:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAAATGACAATTGTAAAATC+AGG	-	chr3.2:88640037-88640056	None:intergenic	25.0%
	AGAAAGCAATTGGAATTGAA+AGG	+	chr3.2:88639861-88639880	MS.gene40233:CDS	30.0%
	ATGACAATTGTAAAATCAGG+AGG	-	chr3.2:88640034-88640053	None:intergenic	30.0%
	ATTGAAAGGAGAAATGTATG+TGG	+	chr3.2:88639875-88639894	MS.gene40233:CDS	30.0%
	GCTTTCTTTCTCAATGAATT+TGG	-	chr3.2:88639848-88639867	None:intergenic	30.0%
	TCATTGAGAAAGAAAGCAAT+TGG	+	chr3.2:88639851-88639870	MS.gene40233:CDS	30.0%
	AAAAATCATCACTGTCGGAT+AGG	+	chr3.2:88639969-88639988	MS.gene40233:CDS	35.0%
!!!	ATCCGACAGTGATGATTTTT+TGG	-	chr3.2:88639969-88639988	None:intergenic	35.0%
!!!	TCCGACAGTGATGATTTTTT+GGG	-	chr3.2:88639968-88639987	None:intergenic	35.0%
	ATTGTAAAATCAGGAGGCAG+TGG	-	chr3.2:88640028-88640047	None:intergenic	40.0%
	GAAAGGAGAAATGTATGTGG+AGG	+	chr3.2:88639878-88639897	MS.gene40233:CDS	40.0%
	GCCCAAAAAATCATCACTGT+CGG	+	chr3.2:88639964-88639983	MS.gene40233:CDS	40.0%
	TTCTGCTGATGCATCATCAA+CGG	+	chr3.2:88640054-88640073	MS.gene40233:CDS	40.0%
!	TGTTGATAGAGTGTTCATCG+AGG	+	chr3.2:88639903-88639922	MS.gene40233:CDS	40.0%
	CATCACTGTCGGATAGGAAT+CGG	+	chr3.2:88639975-88639994	MS.gene40233:CDS	45.0%
!	TGTGCAGTTGCTTTCTTCTG+TGG	-	chr3.2:88639929-88639948	None:intergenic	45.0%
!!	TCATCGGTGTCGAGCATATT+CGG	+	chr3.2:88639803-88639822	MS.gene40233:CDS	45.0%
	CAAGTAGAAGGAGTAGGAGG+CGG	-	chr3.2:88640094-88640113	None:intergenic	50.0%
	CTGCACATCACATGAAGACG+AGG	+	chr3.2:88639942-88639961	MS.gene40233:CDS	50.0%
	GTAAAATCAGGAGGCAGTGG+TGG	-	chr3.2:88640025-88640044	None:intergenic	50.0%
	GTGTCGAGCATATTCGGATG+CGG	+	chr3.2:88639809-88639828	MS.gene40233:CDS	50.0%
	TGATGCATCATCAACGGCCA+CGG	+	chr3.2:88640060-88640079	MS.gene40233:CDS	50.0%
	TGTCGAGCATATTCGGATGC+GGG	+	chr3.2:88639810-88639829	MS.gene40233:CDS	50.0%
!	GGCAAAGGAGCATACTCTGT+TGG	-	chr3.2:88640004-88640023	None:intergenic	50.0%
	GTCGAGCATATTCGGATGCG+GGG	+	chr3.2:88639811-88639830	MS.gene40233:CDS	55.0%
	GTAGAAGGAGTAGGAGGCGG+AGG	-	chr3.2:88640091-88640110	None:intergenic	60.0%
	TCAGGAGGCAGTGGTGGCAA+AGG	-	chr3.2:88640019-88640038	None:intergenic	60.0%
	AGGAGGCGGAGGAACAACCG+TGG	-	chr3.2:88640080-88640099	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	88639800	88640114	88639800	ID=MS.gene40233
chr3.2	mRNA	88639800	88640114	88639800	ID=MS.gene40233.t1;Parent=MS.gene40233
chr3.2	exon	88639800	88640114	88639800	ID=MS.gene40233.t1.exon1;Parent=MS.gene40233.t1
chr3.2	CDS	88639800	88640114	88639800	ID=cds.MS.gene40233.t1;Parent=MS.gene40233.t1

Gene Sequence	>MS.gene40233 ATGTCATCGGTGTCGAGCATATTCGGATGCGGGGTTTCGATTGCGCCAAATTCATTGAGAAAGAAAGCAATTGGAATTGAAAGGAGAAATGTATGTGGAGGATTGTTGATAGAGTGTTCATCGAGGCCACAGAAGAAAGCAACTGCACATCACATGAAGACGAGGCCCAAAAAATCATCACTGTCGGATAGGAATCGGAAACCAACAGAGTATGCTCCTTTGCCACCACTGCCTCCTGATTTTACAATTGTCATTTCTGCTGATGCATCATCAACGGCCACGGTTGTTCCTCCGCCTCCTACTCCTTCTACTTGA
Protein sequence	>MS.gene40233.t1 MSSVSSIFGCGVSIAPNSLRKKAIGIERRNVCGGLLIECSSRPQKKATAHHMKTRPKKSSLSDRNRKPTEYAPLPPLPPDFTIVISADASSTATVVPPPPTPST