AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68723

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68723.t1 MTR_3g118360 96.747 953 25 2 2 954 34 980 0.0 1890
MS.gene68723.t1 MTR_3g118360 96.323 843 25 2 2 844 34 870 0.0 1672
MS.gene68723.t1 MTR_3g118360 96.437 842 24 2 2 843 34 869 0.0 1670
MS.gene68723.t1 MTR_3g118360 99.003 702 7 0 253 954 1 702 0.0 1405
MS.gene68723.t1 MTR_4g074640 35.308 827 445 21 60 828 16 810 2.25e-143 451
MS.gene68723.t1 MTR_4g074670 37.580 471 253 12 60 506 16 469 3.92e-92 306
MS.gene68723.t1 MTR_4g074670 35.880 301 172 7 212 506 2 287 1.71e-46 173
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68723.t1 AT5G22800 73.778 900 233 3 55 952 103 1001 0.0 1381
MS.gene68723.t1 AT5G22800 73.778 900 233 3 55 952 78 976 0.0 1381
MS.gene68723.t1 AT1G50200 37.084 782 407 18 60 785 64 816 1.82e-157 489
MS.gene68723.t1 AT1G50200 37.084 782 407 18 60 785 64 816 2.43e-157 489

Find 219 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTTATCTTTGTGAAAA+AGG 0.152053 3.2:+93233105 MS.gene68723:CDS
GTACCTGACACTGAATTTAT+TGG 0.154294 3.2:+93227710 MS.gene68723:CDS
ACACTAATCCATAATCTTTC+TGG 0.181990 3.2:-93226167 None:intergenic
CAATTCAAACCTATCTTTCT+TGG 0.208066 3.2:+93225948 MS.gene68723:CDS
AATACTTTCTGAGCAATTAT+AGG 0.215096 3.2:-93227311 None:intergenic
ATTCAGGCAGGTAAATTTAT+TGG 0.228211 3.2:+93232967 MS.gene68723:CDS
TACTCCAGGGGTGGTTGGTC+AGG 0.230750 3.2:+93232942 MS.gene68723:CDS
GTTTGCCTCCAGAAAGATTA+TGG 0.233077 3.2:+93226159 MS.gene68723:CDS
AAGTCAGTTTCATAGTTATT+TGG 0.248121 3.2:-93226932 None:intergenic
AAGTGCCGCAGAATATTTAA+TGG 0.255077 3.2:+93232843 MS.gene68723:CDS
ATCCTCACAGTTACTTCTTC+AGG 0.258382 3.2:-93230863 None:intergenic
GACCTCTCAAGCTGGTTCTT+TGG 0.278405 3.2:+93228292 MS.gene68723:CDS
ATAGATGTTCAGAAGTCTTT+TGG 0.278583 3.2:+93227935 MS.gene68723:CDS
TCTATGTTCTTCTGCTTTAG+AGG 0.298105 3.2:-93226624 None:intergenic
TGGGAGGAAGCCTGAAAATT+TGG 0.299980 3.2:+93233050 MS.gene68723:CDS
CCTCCTGATTCAGCATAAAA+TGG 0.304165 3.2:-93227842 None:intergenic
GCTGAATCAGGAGGACAAAT+TGG 0.307268 3.2:+93227851 MS.gene68723:CDS
GGCAGGTTGCTTGGTGTAAA+AGG 0.307397 3.2:+93227254 MS.gene68723:CDS
GGTGGTTGGTCAGGGAATTC+AGG 0.315604 3.2:+93232951 MS.gene68723:CDS
CTACCCGTAACTCCTCTAAA+AGG 0.316525 3.2:-93230892 None:intergenic
GACTTATCTACCATTGAGTT+TGG 0.319082 3.2:+93226137 MS.gene68723:CDS
ACTGTTCTCCTCACCATTGC+TGG 0.323081 3.2:+93225918 MS.gene68723:CDS
GAAGGAGCAATTGCAATGTT+TGG 0.329419 3.2:+93228464 MS.gene68723:CDS
AAGCAGAAGAACATAGATAC+TGG 0.331732 3.2:+93226630 MS.gene68723:CDS
ACTACAAGCTGCGCTTAAAA+AGG 0.334338 3.2:+93228256 MS.gene68723:CDS
CAACCTCTAGAACACCCTTT+TGG 0.351759 3.2:-93227985 None:intergenic
TAACCCCTCCCAACGTTTGA+TGG 0.352810 3.2:-93227194 None:intergenic
AACCTCTTAATTCGGTCAAC+GGG 0.355293 3.2:-93226328 None:intergenic
GTGATCCAGCCGTACAGGTT+AGG 0.360098 3.2:+93227783 MS.gene68723:CDS
CAGGATACTAGTTGAGTGTT+TGG 0.364396 3.2:+93232795 MS.gene68723:intron
AGGAGAACAGTGGGGTCATC+TGG 0.366681 3.2:-93225906 None:intergenic
AACAAGCTATTCAATGGGCT+TGG 0.372665 3.2:+93226114 MS.gene68723:CDS
TTACCTGTTGAGACCATAAC+TGG 0.378573 3.2:-93226214 None:intergenic
CTTTCAGATACCTCTTTGTC+TGG 0.392755 3.2:-93227018 None:intergenic
GAATTAGCAAATATTTCATA+TGG 0.402355 3.2:+93226983 MS.gene68723:CDS
GTCAAGCTTGCCATTGGAAG+CGG 0.403782 3.2:+93227668 MS.gene68723:CDS
CATAGATACTGGGCTTGGTC+TGG 0.405421 3.2:+93226641 MS.gene68723:CDS
ATCATGAGACTTTCCAGTTA+TGG 0.406423 3.2:+93226201 MS.gene68723:CDS
GTAGCCAGCCTAGTGGTAAA+TGG 0.407529 3.2:+93227761 MS.gene68723:CDS
CCCACCAGCCTGAGCGAAGT+TGG 0.409388 3.2:-93233031 None:intergenic
TGGTGGCATTTGATCGTCTC+AGG 0.410015 3.2:+93228312 MS.gene68723:CDS
CTTCACCCAACCTCTTAATT+CGG 0.423735 3.2:-93226336 None:intergenic
GTACGTGTTGTAGAGGTTCC+TGG 0.424531 3.2:+93229618 MS.gene68723:CDS
ATTATCTCCACGACAGCCTT+AGG 0.424823 3.2:-93227914 None:intergenic
TTTCCAGTTATGGTCTCAAC+AGG 0.427549 3.2:+93226211 MS.gene68723:CDS
ACTCCAGGGGTGGTTGGTCA+GGG 0.433011 3.2:+93232943 MS.gene68723:CDS
CATACCTTCTTTGAGATGCT+TGG 0.433272 3.2:+93226062 MS.gene68723:CDS
TTATGTGGTGGTGGAGGAGG+TGG 0.436304 3.2:+93233003 MS.gene68723:CDS
TGGGTGAAGAGGATAACTTT+TGG 0.438035 3.2:+93226350 MS.gene68723:CDS
TCAGAGCAGGGTATTGCATC+TGG 0.441838 3.2:+93229711 MS.gene68723:CDS
GGAGGACAAATTGGAGATCA+TGG 0.446126 3.2:+93227860 MS.gene68723:CDS
CTCTCAAGCTGGTTCTTTGG+TGG 0.451414 3.2:+93228295 MS.gene68723:CDS
TTCAGAGGCCTTCTCTATGA+TGG 0.452809 3.2:-93226963 None:intergenic
AGGCGAATAGAAGCTGTAGC+TGG 0.454459 3.2:+93229738 MS.gene68723:CDS
ATGCTTGGTAACTTCAGCTT+TGG 0.456959 3.2:+93226077 MS.gene68723:CDS
ATTGTCCTGGGCTCATGTCC+AGG 0.459686 3.2:+93232889 MS.gene68723:CDS
TATCCAATAAATTCAGTGTC+AGG 0.460034 3.2:-93227713 None:intergenic
AGGGTATTGCATCTGGTGTC+AGG 0.461565 3.2:+93229718 MS.gene68723:CDS
CTTCAACTCCTCAAGGATAC+GGG 0.462475 3.2:-93227378 None:intergenic
TGATCCAGCCGTACAGGTTA+GGG 0.465370 3.2:+93227784 MS.gene68723:CDS
AGTAATACTTCTGAAATTCG+TGG 0.467614 3.2:+93229675 MS.gene68723:CDS
TCACAAGGGTACAGTCCAAA+AGG 0.467810 3.2:+93227970 MS.gene68723:CDS
GGAACTGTACCTTGCTTGGC+TGG 0.471150 3.2:+93227494 MS.gene68723:CDS
ATTCCCTGACCAACCACCCC+TGG 0.472687 3.2:-93232946 None:intergenic
CATGGCTCGCATTCTTCAAA+AGG 0.478575 3.2:+93226668 MS.gene68723:CDS
ACAAGCTATTCAATGGGCTT+GGG 0.479029 3.2:+93226115 MS.gene68723:CDS
GGTAGCAAGAAACGAAAATT+CGG 0.480512 3.2:+93230910 MS.gene68723:CDS
TCCCCGTTGACCGAATTAAG+AGG 0.480923 3.2:+93226326 MS.gene68723:CDS
GTTCGTACAGGCAGGTTGCT+TGG 0.481856 3.2:+93227245 MS.gene68723:CDS
TCTTCAACTCCTCAAGGATA+CGG 0.483308 3.2:-93227379 None:intergenic
TCCAACTTCGCTCAGGCTGG+TGG 0.485257 3.2:+93233030 MS.gene68723:CDS
GGGTGTTGAAAGCAGGATCT+TGG 0.487566 3.2:+93226525 MS.gene68723:intron
AAATTATGTGGTGGTGGAGG+AGG 0.487864 3.2:+93233000 MS.gene68723:CDS
TTTCTGCAAGCTCGCTATCA+AGG 0.488467 3.2:-93228361 None:intergenic
ACATCGGCGTATCCCCTCTC+AGG 0.490257 3.2:-93226430 None:intergenic
AGAGCAAGGACCGCAGGGAC+CGG 0.491524 3.2:-93226386 None:intergenic
GTTGACCGAATTAAGAGGTT+GGG 0.494052 3.2:+93226331 MS.gene68723:CDS
AAATGGTGATCCAGCCGTAC+AGG 0.500392 3.2:+93227778 MS.gene68723:CDS
ATTGTGTTTCTCATCTCTGA+TGG 0.502765 3.2:+93227164 MS.gene68723:CDS
ATCAGAAGAGTTGTTCGTAC+AGG 0.503028 3.2:+93227233 MS.gene68723:CDS
AATAATAATAATTGTGTGAG+TGG 0.505763 3.2:+93225807 MS.gene68723:CDS
GGCGAATAGAAGCTGTAGCT+GGG 0.506427 3.2:+93229739 MS.gene68723:CDS
GAAGAGTTGTTCGTACAGGC+AGG 0.512237 3.2:+93227237 MS.gene68723:CDS
TGATACTTATGGTTTCCCTA+TGG 0.515111 3.2:+93227538 MS.gene68723:CDS
CATTGTTGATATCATTAGTA+CGG 0.517063 3.2:-93226019 None:intergenic
TGGTGTAAAAGGTGATGGTA+GGG 0.517675 3.2:+93227265 MS.gene68723:CDS
TCAGAGGCCTTCTCTATGAT+GGG 0.518722 3.2:-93226962 None:intergenic
GTACCATCAAACGTTGGGAG+GGG 0.519236 3.2:+93227191 MS.gene68723:CDS
GCAAAATTATGTGGTGGTGG+AGG 0.520739 3.2:+93232997 MS.gene68723:CDS
GTTACCAAGCATCTCAAAGA+AGG 0.522266 3.2:-93226066 None:intergenic
CAAAAGGGTGTTCTAGAGGT+TGG 0.522615 3.2:+93227986 MS.gene68723:CDS
CTCTGATTTAGCTTTCTCGA+GGG 0.522771 3.2:-93233079 None:intergenic
AATTTCAGAGCAAGGACCGC+AGG 0.525249 3.2:-93226392 None:intergenic
CCTTCCCTAACCTGTACGGC+TGG 0.530381 3.2:-93227788 None:intergenic
ACTAAGAAATCTTTCCCGTA+TGG 0.530405 3.2:-93225835 None:intergenic
AGGTCCAACTTCGCTCAGGC+TGG 0.530546 3.2:+93233027 MS.gene68723:CDS
CTTCACCTGGACATGAGCCC+AGG 0.531541 3.2:-93232894 None:intergenic
GTGTTGTACCATCAAACGTT+GGG 0.531789 3.2:+93227186 MS.gene68723:CDS
TGGGCTTGGTCTGGAGCGCA+TGG 0.533587 3.2:+93226650 MS.gene68723:CDS
GCTTCGATTTGTGCAGACGC+TGG 0.536077 3.2:+93227409 MS.gene68723:CDS
TGGAAGGTCCAACTTCGCTC+AGG 0.537180 3.2:+93233023 MS.gene68723:CDS
AATACTGTCAAGCTTGCCAT+TGG 0.538095 3.2:+93227662 MS.gene68723:CDS
GAGCCCAGGACAATAGCTGC+TGG 0.539000 3.2:-93232880 None:intergenic
GAATTGAAGCATTCCAGCAA+TGG 0.539001 3.2:-93225931 None:intergenic
CTGGATCACCATTTACCACT+AGG 0.539915 3.2:-93227769 None:intergenic
GGAGGGGTTATGTAGTTCGA+CGG 0.540107 3.2:+93227207 MS.gene68723:CDS
CTGGGCTCATGTCCAGGTGA+AGG 0.540402 3.2:+93232895 MS.gene68723:CDS
AAGAACATAGATACTGGGCT+TGG 0.541349 3.2:+93226636 MS.gene68723:CDS
AGACTAACCTTCCCTTCACC+TGG 0.541694 3.2:-93232907 None:intergenic
GGAAAGTCTCATGATCGTCT+TGG 0.542083 3.2:-93226193 None:intergenic
TTTAAAGTAATTTCAGAGCA+GGG 0.545061 3.2:+93229699 MS.gene68723:CDS
AGATCCAGCAGCTATTGTCC+TGG 0.546412 3.2:+93232876 MS.gene68723:CDS
TCAAGCTTGCCATTGGAAGC+GGG 0.546840 3.2:+93227669 MS.gene68723:CDS
CGTTGACCGAATTAAGAGGT+TGG 0.549152 3.2:+93226330 MS.gene68723:CDS
TTGGTGTAAAAGGTGATGGT+AGG 0.549614 3.2:+93227264 MS.gene68723:CDS
GATCCAGCAGCTATTGTCCT+GGG 0.550834 3.2:+93232877 MS.gene68723:CDS
TTATGCTTCCCGTGGTCACA+AGG 0.553002 3.2:+93225860 MS.gene68723:CDS
GTTGGTCAGGGAATTCAGGC+AGG 0.558788 3.2:+93232955 MS.gene68723:CDS
CACAAGGGTACAGTCCAAAA+GGG 0.558794 3.2:+93227971 MS.gene68723:CDS
GGTCAGGAGACCTCTCAAGC+TGG 0.560850 3.2:+93228284 MS.gene68723:CDS
ATATCAAGTGCTGAAAGAAG+TGG 0.561924 3.2:+93227473 MS.gene68723:CDS
GTAGCACGAGGGACTTGTCT+AGG 0.562012 3.2:-93225978 None:intergenic
TGGAGGCAAACCAAACTCAA+TGG 0.563536 3.2:-93226147 None:intergenic
CCAACTTCGCTCAGGCTGGT+GGG 0.563594 3.2:+93233031 MS.gene68723:CDS
ACTCCCTTCCCTAACCTGTA+CGG 0.564727 3.2:-93227792 None:intergenic
TGGCAATATATTTGTTCACA+AGG 0.567482 3.2:+93227955 MS.gene68723:CDS
ATCAGCAAGAGGCATCACTT+TGG 0.568344 3.2:-93228432 None:intergenic
GGTGTAAAAGGTGATGGTAG+GGG 0.568681 3.2:+93227266 MS.gene68723:CDS
AATTAAGAGGTTGGGTGAAG+AGG 0.568929 3.2:+93226339 MS.gene68723:CDS
AATATTTGTCCCGCTTCCAA+TGG 0.570926 3.2:-93227678 None:intergenic
ATAGCAAAATTATGTGGTGG+TGG 0.571171 3.2:+93232994 MS.gene68723:CDS
TCATAATAAATTTCAGAGCA+AGG 0.571821 3.2:-93226400 None:intergenic
AAAGTTGAGGCAACGAGCCA+AGG 0.572093 3.2:+93228036 MS.gene68723:CDS
CAACCTCTTAATTCGGTCAA+CGG 0.573322 3.2:-93226329 None:intergenic
CACCAAAGAACCAGCTTGAG+AGG 0.573822 3.2:-93228294 None:intergenic
CTTCTCTCTTCAACTCCTCA+AGG 0.574968 3.2:-93227385 None:intergenic
GTGTGAGTGGAGATTCCATA+CGG 0.575858 3.2:+93225820 MS.gene68723:CDS
TTGCGACGATGATGTACAGA+AGG 0.575956 3.2:-93225748 None:intergenic
ATCACCATTTACCACTAGGC+TGG 0.578306 3.2:-93227765 None:intergenic
TGAAATATTTGCTAATTCAG+AGG 0.579198 3.2:-93226978 None:intergenic
ACTAATGATATCAACAATGT+TGG 0.579654 3.2:+93226023 MS.gene68723:CDS
TATGGAAATAACAAAAGAAG+TGG 0.579964 3.2:+93227556 MS.gene68723:CDS
CTCATGTCCAGGTGAAGGGA+AGG 0.580776 3.2:+93232900 MS.gene68723:CDS
GTGGTGGTGGAGGAGGTGGA+AGG 0.581825 3.2:+93233007 MS.gene68723:CDS
TGGGCTCATGTCCAGGTGAA+GGG 0.582195 3.2:+93232896 MS.gene68723:CDS
AGCAGAAGAACATAGATACT+GGG 0.583073 3.2:+93226631 MS.gene68723:CDS
AGGAAGGACCTTGTGACCAC+GGG 0.584439 3.2:-93225868 None:intergenic
GCAATGGTGAGGAGAACAGT+GGG 0.584917 3.2:-93225915 None:intergenic
AAGTGGAACTGTACCTTGCT+TGG 0.584929 3.2:+93227490 MS.gene68723:CDS
TGTACCATCAAACGTTGGGA+GGG 0.587357 3.2:+93227190 MS.gene68723:CDS
TTGTACCATCAAACGTTGGG+AGG 0.587433 3.2:+93227189 MS.gene68723:CDS
GCAGTAGCAGCAGCAGCAGG+AGG 0.588218 3.2:+93225675 MS.gene68723:CDS
GAGAGGAGAGAAATTGCTTG+AGG 0.590060 3.2:+93227433 MS.gene68723:CDS
GGCAATATATTTGTTCACAA+GGG 0.592455 3.2:+93227956 MS.gene68723:CDS
TGGGGCATTCAAAACAGTAC+AGG 0.592610 3.2:+93230993 MS.gene68723:CDS
CAAACAAGTGGAAGCAACAG+TGG 0.594498 3.2:+93228009 MS.gene68723:CDS
TTAGCAGTAGCAGCAGCAGC+AGG 0.595741 3.2:+93225672 MS.gene68723:CDS
GCTGATCAACGGATGGATTG+AGG 0.596209 3.2:+93228391 MS.gene68723:CDS
AGAGGTTCCTGGAGTATCGA+TGG 0.596716 3.2:+93229629 MS.gene68723:CDS
AGTCCAAAAGGGTGTTCTAG+AGG 0.604342 3.2:+93227982 MS.gene68723:CDS
AGGAAGCCTGAAAATTTGGC+TGG 0.604884 3.2:+93233054 MS.gene68723:CDS
TTTGTGCAGACGCTGGAGAG+AGG 0.605643 3.2:+93227416 MS.gene68723:CDS
TGTTTCCATTAAATATTCTG+CGG 0.605968 3.2:-93232848 None:intergenic
CAAAGTTCCATCGATACTCC+AGG 0.606263 3.2:-93229636 None:intergenic
CGCTATCAAGGAGTGGACGG+TGG 0.606601 3.2:-93228349 None:intergenic
AGGATCTTGGTGATGATACA+CGG 0.607079 3.2:+93226538 MS.gene68723:intron
GATTCTCCCCATCATAGAGA+AGG 0.608580 3.2:+93226955 MS.gene68723:CDS
GGACAGATAGCAAAATTATG+TGG 0.609892 3.2:+93232988 MS.gene68723:CDS
GCTCTGATTTAGCTTTCTCG+AGG 0.610643 3.2:-93233080 None:intergenic
GAAATCGAACTGCTGATCAA+CGG 0.611385 3.2:+93228380 MS.gene68723:CDS
CCAGCCGTACAGGTTAGGGA+AGG 0.611758 3.2:+93227788 MS.gene68723:CDS
GAACGAGAGAAGCACTAGGA+AGG 0.612505 3.2:-93225884 None:intergenic
TCAGGAACATACTTTGAGAG+TGG 0.613102 3.2:-93229817 None:intergenic
TCGAACTGCTGATCAACGGA+TGG 0.614833 3.2:+93228384 MS.gene68723:CDS
GAATCAACCTAAGGCTGTCG+TGG 0.615063 3.2:+93227907 MS.gene68723:CDS
ATTTCAGAGCAAGGACCGCA+GGG 0.616018 3.2:-93226391 None:intergenic
AGAGGGTGAGAATCAACCTA+AGG 0.616634 3.2:+93227898 MS.gene68723:CDS
TAGGAAGGACCTTGTGACCA+CGG 0.617961 3.2:-93225869 None:intergenic
ACTTCGCTCAGGCTGGTGGG+AGG 0.619056 3.2:+93233034 MS.gene68723:CDS
GGAGTATCGATGGAACTTTG+TGG 0.622057 3.2:+93229639 MS.gene68723:CDS
CTAATCCATAATCTTTCTGG+AGG 0.622240 3.2:-93226164 None:intergenic
GCTCGCTATCAAGGAGTGGA+CGG 0.625144 3.2:-93228352 None:intergenic
TGAAGGGAAGGTTAGTCTAG+TGG 0.626264 3.2:+93232912 MS.gene68723:CDS
TCTAGAGGTTGGCAAACAAG+TGG 0.626675 3.2:+93227997 MS.gene68723:CDS
AGCAATGGTGAGGAGAACAG+TGG 0.627721 3.2:-93225916 None:intergenic
GGTTAGGGAAGGGAGTAATG+TGG 0.629772 3.2:+93227799 MS.gene68723:CDS
GTGTTTGGATGATGTTGATG+CGG 0.629997 3.2:+93232810 MS.gene68723:CDS
GGTGTGAGTATTGACATGAA+TGG 0.634865 3.2:+93227590 MS.gene68723:CDS
TATCATACTACTTACAACAT+CGG 0.636211 3.2:-93226446 None:intergenic
TTGCTTGGTGTAAAAGGTGA+TGG 0.638860 3.2:+93227260 MS.gene68723:CDS
GAAGCATTCCAGCAATGGTG+AGG 0.639478 3.2:-93225926 None:intergenic
GCAAGCTCGCTATCAAGGAG+TGG 0.639731 3.2:-93228356 None:intergenic
CGTTGTGAAGTAGTAGCACG+AGG 0.644204 3.2:-93225990 None:intergenic
GATGGTAGGGGAGACCCTGA+AGG 0.644605 3.2:+93227278 MS.gene68723:CDS
ACTGCAGGTACGTGTTGTAG+AGG 0.644859 3.2:+93229611 MS.gene68723:intron
TATCGATGGAACTTTGTGGT+GGG 0.645529 3.2:+93229643 MS.gene68723:CDS
CAGCCGTACAGGTTAGGGAA+GGG 0.646194 3.2:+93227789 MS.gene68723:CDS
AGCGGAGTCACCGGTCCCTG+CGG 0.646446 3.2:+93226376 MS.gene68723:CDS
TAGAGCACCCCGTATCCTTG+AGG 0.648075 3.2:+93227370 MS.gene68723:CDS
CAACAGTGGATGTAAAGTTG+AGG 0.654944 3.2:+93228023 MS.gene68723:CDS
AGCTTGTAGTAAATGAGTAG+CGG 0.656398 3.2:-93228243 None:intergenic
TGTGAGTGGAGATTCCATAC+GGG 0.656667 3.2:+93225821 MS.gene68723:CDS
CAGAGGCCTTCTCTATGATG+GGG 0.657776 3.2:-93226961 None:intergenic
GCGAATAGAAGCTGTAGCTG+GGG 0.661208 3.2:+93229740 MS.gene68723:CDS
TATCTGATGACCAGACAAAG+AGG 0.661772 3.2:+93227008 MS.gene68723:CDS
TTCAACTCCTCAAGGATACG+GGG 0.663705 3.2:-93227377 None:intergenic
AAAGAAGGTATGATGACGTG+AGG 0.668436 3.2:-93226051 None:intergenic
ACCTCTTAATTCGGTCAACG+GGG 0.679351 3.2:-93226327 None:intergenic
ACATCTTCACCAGCCAAGCA+AGG 0.684496 3.2:-93227503 None:intergenic
AAAGAAGTGGCTGAAGAACG+CGG 0.692053 3.2:+93227569 MS.gene68723:CDS
TGCGACGATGATGTACAGAA+GGG 0.698896 3.2:-93225747 None:intergenic
GGTGTTGTACCATCAAACGT+TGG 0.701946 3.2:+93227185 MS.gene68723:CDS
AGCAATTGTAGCCAGCCTAG+TGG 0.702322 3.2:+93227754 MS.gene68723:CDS
GTATCGATGGAACTTTGTGG+TGG 0.704292 3.2:+93229642 MS.gene68723:CDS
TCTGGAACGAGAGAAGCACT+AGG 0.708872 3.2:-93225888 None:intergenic
AACCTGAAGAAGTAACTGTG+AGG 0.719116 3.2:+93230861 MS.gene68723:CDS
CAGATAGCAAAATTATGTGG+TGG 0.721808 3.2:+93232991 MS.gene68723:CDS
GTTGTGAAGTAGTAGCACGA+GGG 0.763537 3.2:-93225989 None:intergenic
CAATGGTGAGGAGAACAGTG+GGG 0.772634 3.2:-93225914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.2 gene 93225660 93233134 93225660 ID=MS.gene68723
chr3.2 mRNA 93225660 93233134 93225660 ID=MS.gene68723.t1;Parent=MS.gene68723
chr3.2 exon 93225660 93226232 93225660 ID=MS.gene68723.t1.exon1;Parent=MS.gene68723.t1
chr3.2 CDS 93225660 93226232 93225660 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93226319 93226453 93226319 ID=MS.gene68723.t1.exon2;Parent=MS.gene68723.t1
chr3.2 CDS 93226319 93226453 93226319 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93226540 93226689 93226540 ID=MS.gene68723.t1.exon3;Parent=MS.gene68723.t1
chr3.2 CDS 93226540 93226689 93226540 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93226929 93227039 93226929 ID=MS.gene68723.t1.exon4;Parent=MS.gene68723.t1
chr3.2 CDS 93226929 93227039 93226929 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93227140 93228057 93227140 ID=MS.gene68723.t1.exon5;Parent=MS.gene68723.t1
chr3.2 CDS 93227140 93228057 93227140 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93228233 93228505 93228233 ID=MS.gene68723.t1.exon6;Parent=MS.gene68723.t1
chr3.2 CDS 93228233 93228505 93228233 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93229618 93229827 93229618 ID=MS.gene68723.t1.exon7;Parent=MS.gene68723.t1
chr3.2 CDS 93229618 93229827 93229618 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93230857 93231014 93230857 ID=MS.gene68723.t1.exon8;Parent=MS.gene68723.t1
chr3.2 CDS 93230857 93231014 93230857 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
chr3.2 exon 93232798 93233134 93232798 ID=MS.gene68723.t1.exon9;Parent=MS.gene68723.t1
chr3.2 CDS 93232798 93233134 93232798 ID=cds.MS.gene68723.t1;Parent=MS.gene68723.t1
Gene Sequence

>MS.gene68723

ATGAATGTTACATTAGCAGTAGCAGCAGCAGCAGGAGGATTTATCTCTTTCAAACAACTACATCTACGTCGTAGTCGTCGTCTTCTTCCCTTCTGTACATCATCGTCGCAAGACAATGATAATAATAATAATAATAATAATAATAATAATAATAATAATTGTGTGAGTGGAGATTCCATACGGGAAAGATTTCTTAGTTTTTATGCTTCCCGTGGTCACAAGGTCCTTCCTAGTGCTTCTCTCGTTCCAGATGACCCCACTGTTCTCCTCACCATTGCTGGAATGCTTCAATTCAAACCTATCTTTCTTGGAAAACTTCCTAGACAAGTCCCTCGTGCTACTACTTCACAACGCTGCATCCGTACTAATGATATCAACAATGTTGGTCATACCTCACGTCATCATACCTTCTTTGAGATGCTTGGTAACTTCAGCTTTGGTGATTACTTCAAAAAACAAGCTATTCAATGGGCTTGGGACTTATCTACCATTGAGTTTGGTTTGCCTCCAGAAAGATTATGGATTAGTGTTTACCAAGACGATCATGAGACTTTCCAGTTATGGTCTCAACAGGTAATTACATCACAATCACATTTCCTTTTAATTACCCCTCTCCTCTTCCGTTCCTGTCGTCACATCACATCTTCATTTCTCTGCAGGTTGGTGTCCCCGTTGACCGAATTAAGAGGTTGGGTGAAGAGGATAACTTTTGGACAAGCGGAGTCACCGGTCCCTGCGGTCCTTGCTCTGAAATTTATTATGATTTTCATCCTGAGAGGGGATACGCCGATGTTGTAAGTAGTATGATATACTTGTTACAAATTGAGTTATTTCAAATCACTTCACTGACAATGTTTTTTGGCTTGGGTGTTGAAAGCAGGATCTTGGTGATGATACACGGTTTATAGAGTTCTACAATTTAGTATTCATGCAATACAACAAAAAAGACGATGGTTCTCTCGAACCTCTAAAGCAGAAGAACATAGATACTGGGCTTGGTCTGGAGCGCATGGCTCGCATTCTTCAAAAGGTATTTCATTCTTTTGAATTGTTGTAAAAGTGAGTGAGTATTTGTACTTTTACAAGTGTTTCTAGGAGACTAGAACTAGAACTATGATATTTCTGCACCCTACACCACATCGTCGCTTAATGTTGAGAAAAGTGGGGCAAGGAAAAAGGGTGTGAATGAAACATTACTCACAAATCACAATACTCTCATTTTCATTTGCTCCTTAAGTCCTTTTTATATTTATTCATTATTCATTGCAGGCTCCAAATAACTATGAAACTGACTTGATTCTCCCCATCATAGAGAAGGCCTCTGAATTAGCAAATATTTCATATGGCATATCTGATGACCAGACAAAGAGGTATCTGAAAGTAAGTGTGACTATCTTCATACAAATTGTGGTAGAATTTTAAATAGTTTATTTCAATCCGCCTTATCACTTTTAATTTATGCTGTTTTTCCTTTACTTAGATTGTAGGAGATCACATGCGTGCAATTGTGTTTCTCATCTCTGATGGTGTTGTACCATCAAACGTTGGGAGGGGTTATGTAGTTCGACGGCTTATCAGAAGAGTTGTTCGTACAGGCAGGTTGCTTGGTGTAAAAGGTGATGGTAGGGGAGACCCTGAAGGAGCATTTTTACCTATAATTGCTCAGAAAGTATTAGAGTTAAGCACTCACATTGATGCTGATGTGAAGAATAGAGCACCCCGTATCCTTGAGGAGTTGAAGAGAGAAGAGCTTCGATTTGTGCAGACGCTGGAGAGAGGAGAGAAATTGCTTGAGGAGAAGCTTGTTGATGCTATATCAAGTGCTGAAAGAAGTGGAACTGTACCTTGCTTGGCTGGTGAAGATGTGTTTATTTTGTATGATACTTATGGTTTCCCTATGGAAATAACAAAAGAAGTGGCTGAAGAACGCGGTGTGAGTATTGACATGAATGGTTTTGATATTGAGATGGAGAAGCAAAGACGTCAATCTCAAGCTGCACACAATACTGTCAAGCTTGCCATTGGAAGCGGGACAAATATTGCAGACAATGTACCTGACACTGAATTTATTGGATATGAAAGTCTTTATTGTAAAGCAATTGTAGCCAGCCTAGTGGTAAATGGTGATCCAGCCGTACAGGTTAGGGAAGGGAGTAATGTGGAAATTTTGCTGAACAAGACTCCATTTTATGCTGAATCAGGAGGACAAATTGGAGATCATGGTTTTCTGTATATATCAGAGGGTGAGAATCAACCTAAGGCTGTCGTGGAGATAATAGATGTTCAGAAGTCTTTTGGCAATATATTTGTTCACAAGGGTACAGTCCAAAAGGGTGTTCTAGAGGTTGGCAAACAAGTGGAAGCAACAGTGGATGTAAAGTTGAGGCAACGAGCCAAGGTACAAAGACTGTGTACCCACTCATTTTATTTATTTACTTCTTTGGTGAATCTTAAGTCATAAATAACAGAATTTGATACGGACAAAAATTTACATCATCATAGTTATGCCTATTTCCAAATCCGAAGCTTACAAGTTGTTCAAGATTACAGTAAGTATAATTGGTGCTTTGCAGGTTCATCATACCGCTACTCATTTACTACAAGCTGCGCTTAAAAAGGTCATCGGTCAGGAGACCTCTCAAGCTGGTTCTTTGGTGGCATTTGATCGTCTCAGGTTTGATTTCAATTTCCACCGTCCACTCCTTGATAGCGAGCTTGCAGAAATCGAACTGCTGATCAACGGATGGATTGAGGATGCGATTCTTCTTCAGACCAAAGTGATGCCTCTTGCTGATGCAAAAAGAGAAGGAGCAATTGCAATGTTTGGAGAAAAATACGGGGAAGAGGTTGGACTTGCTACAAACGCTTTTGATATTTAACTTTGAAATTAATTTAGCTTACTTCTGTTTTGGTAGTTTTAAACATGAAGTGTGTACATAAACGAGCTCAGAAATTTGACCTTCAGGGATGGATTGCAACTAATGTTCAGGAATCATCATGTCTTCTGACCTAAATTCCCACATTACTATTACTATTGCCAGATTCCAATTACATTGTGATTTCAGATGCATCAATAGTTATCCCTCTCTCTCTATCACTTGTACCAGCACCTACACATGGACCATAGTAGTGAAATATACGGCATAGTAATATACTAGATTCTTAAAAAAAATTAAGAGCATCCAGCTGTGAAGAATTCTTAAACTGTACAAACGAAATGTAATCCCTAACAGCCCACAACCATGTCTACATCTAAGTTTGGTTATGACTTTGATTGGCAAAATTTTATTATTAGTGGCAGCTAGTGCCTAGTTGTTAGCAGCTTGGCTTATAGCTAAAAGTGGCCATGCTAACTGTCTGATGTTAAAGTATCTAATAAAGTTAGTGGTAAACTCGCTGATAAATATAAAATGACATGTCTATCTTTTTTTAGATAAATTGTGTTTATGTGTAGTATTGATTTGTAAATTATTATCGCTTTTAATACTTAAATTTATTTAATATACTTCTCATTTCTACATAAAATGCGATAATGCTAACAAAATGACATGTACACATAATTCTTTCATATAAATTTTAAGATTTTACAGGAAAATATATACTCTCCAGGGGCTAGGATTCTTTTGTTTTAAAGGGAGTATAATTAATATATTGAGAGTAAAAAGAAAAAAGAAAATAATACAATACAAACTATAGGCTCAGATGCTATATGAAATATAACACAAAAATAACCCTATAAAGTAGTGGGATAAGTTTCTTTATCAAACAACTCTAGTTATTAAATCCACTATAAACCAGTCAGGTCCACCGTAAGCTACTGGATCAAAGTTGCCAATGAGATTATATATGTTCCATATTTTAGACATTTATATTTCTACTTCTCAATAATCGGCGAAAATAACCTGTTACTTCTCAATAACCTGTTTCTTCTTGTGTAATCATATTTGGTGTACTGCAGGTACGTGTTGTAGAGGTTCCTGGAGTATCGATGGAACTTTGTGGTGGGACTCATGTCAGTAATACTTCTGAAATTCGTGGTTTTAAAGTAATTTCAGAGCAGGGTATTGCATCTGGTGTCAGGCGAATAGAAGCTGTAGCTGGGGAAGCTTTCATCGAATATGTCAATGCTCGAGATTTATATCTGAAACAGCTATGTTCCACTCTCAAAGTATGTTCCTGATTTCAGTGTTTCAGAATTTTGTGCAAATAGTATAAATTTGTACAGAGTAGTTAATATCCATGACCACGAGTCATGCAATTGATATGACCAATACTTAATAATATAAATTTGTGCGCATAGGTATCTGTTTGTTTTAAGGGGGACCCTTGGCACAACGGTAAAGTTGTTGTCATGTGACTAAAAGGTCACCGGTTCAAGTCCTATAAACAGCCTCTTGTGTAAAAAACAGGTTAAGGCTGCGTATAATACACCAAAATGGCGGGACCCCTTCTCGGACCCTGCGTATGTGGGAGCTTTTTTGCACCGGGCTGCCTTTTATAGGTATCTGTTTGTTCTCCTTGCCCTTTGTTGGGGTTGAGTACACAGAAGAATTAGGGTTGAGAGACAAACCATTATATTTTTTACAAGATGGGATTCAATCACAATATGGGTAGGGTTTTCCATGCGGGTGTGGCTCAGTTGGTTATGTAGCATTGAGTTTATGGGGGAGGACCAAGATTCGATCCCCGCAGAACACACTATTGGGGAGGGGTGAAGAGCTTTCAGCTGCAACCTAGGCACTCGAGCGGAGATTAGTCCCATTGCTTGTCCAAAGGACTGAAGTCTGCTGTGATACTCTGTGGAGCCACTGAGGTGTCAAAGGCCCTAATTACAGCGTTTTCTTACCAAAGCCAAAATATTTACTACGACATTGCCATGCCTTACAAAGATTTTGTAGTTTGTTTCCTTGTGTATTATACATATAAGATAAGGGAACCGTCTCACTTTTTTCATGCTCGCACATTTGGGGTTCCTTGTTGCATGTGTTGCTCAAATACATAGAGTAGTATTTCCTTTATGTCTATAATAATCTTTAACATGAATTTTTAATAAAAAAGGGCAGCCCAGTGCACTAAAGCTCCCACATACGCAGGGTCCGGGAAAGGGGTCCTACCATTTTGGTGTATTGTAGATTTTGACGTTTTCTGCATCTGGTACCATTTCTATCCTTGAATAACTTATAATCAACTTTTGAAGGTTAAACCTGAAGAAGTAACTGTGAGGATAGAAAACCTTTTAGAGGAGTTACGGGTAGCAAGAAACGAAAATTCGGCTGTGCGTGCAAAAGCAGCAGTCTACAAAGCTTCAGTTATTGCAAGCAGAGCGTTTTTGGTGGGGCATTCAAAACAGTACAGGTAATACTTCTCCATCTTGTAGTATTTGTTACTTTAATGTGGGTTTGGGAGAGAACCTTCTCCTTGTTAGCAACACCAAAAAAAGTTTTTCTGATTAGATAATATATGCCTGTAAGGTACAAACTATTGTATATAACTGGGGAAGAAGGTGAAAAATATTTCCTTGATATAATGTCATGTCATAACCAACTGAAATCCAACAATTGGAAACTGATCCAGATATCTATGAAAAGGGAGCCCAAAATAAGTTTAACAAATAAATATTAAATAAACAATTAATTATAACAGTATAATACAATTAAAAAACTAATAATAAATTTTTAATGAACTCCATACATACTCAAAATATAGCAGCTCGAAACTCTAAATTCGTTAGGGAAGTGTGCAACGGAGGAAGGCTTAATGGGTGTTCTATTTTTTTCCCAATAGCACTTATATTATAAGTGCTGTGGTACTCTATTTCTAAAGTTAAAAAAGAGTTCATGTAATTAGGAGGAAAAAAATGGAAGTTGATTAATTTAACTCCCATGCAAAAGGCTAGTTTTGAATAAAATTAATAGATGCCAAAAGCTTGGATGAATTGAGCTAATGTATATTGTTATTCATACTCAAGCATATAAATCTGCATTCACTTCGTCAATTTGATTTTCTTCTAATTAGGATTATTCAAGAGATTGGCGTTGATAATTGTTAAAACAATAAAACCTAAAAATTAATTTATTCCTAATTAAATTAACCATTCAAAGGTAGCCCAATAACTGCTAAAACAAAAGTTGTCTGAGCAGGGACAGATTGGAAAGGGAAATGCACGGCCCCTTCCATCTCCCCTTTTCTCGTTTGCAGATACAAACCTCCTGGGTGAAAGGCGATATTCCTATTCTAGCAGCGGAATTTCTGCTTCAGATATAAAAGTCTAACTTTTCACACCCGGCTTGTGCGCTTTTGCTTTAGGAAACTCCACCAACGACAAAATCTTTATCAACTATTTAGAGGAGCACAAGCTTTTCACCTTTACCAACTCAGTAAAGCTCCAAAGTTCATCTGTTTCAACGGATGGCCCCCTAAATATTATTTAACTATTATAAATTAATATTCATGTGATATTTATAATCATATATTTTTTGACCTCTCATATGATATAGTTTTGGATCTGTCCGTGTTTGAGTGCAAACTATTCTGATGGTTATCCATATAACTTTAGTAATACCGACTGGAATGATATGCCATGTCATAGAAGTAGAATGACATGGGACAAGGGTAGGTTACACACATGCGTGTCCCCCCTCCCCTGGGTTCGTCTCTGCCGGAAAAATAGAAGCAGAGGTCTTGCACATCATTGATGTTCCCTCGAATATATTTTCTGTGAATGCTGCTGGCATAACTAGAACATGGGAGGATGTCTTTCATATGTGTATTTCCACGAAAGGGTGACTTGGTGCTAAAACTCCCGCACCCTTGTGGGTTTCAGGATAGAGTCAGACCCATTGTGAGTTTGATAAAGGGCAACCTAATCGTAGTGTGTTATAAAGCTCCCGCGGTGGGCTACGGGGGCTTGCACACGCACAGGTCTGTTGCAGGTAATCTTTCCTGGCACCTAACCTTTGACCTCGTAATCAACACTACAATAACTCTAACTATTGTACACAAACTTTCCTTCATTGTGCGTCTACTGTATATACAGGACTGGAAATGGAAAAGAAAAATTATGGTTGAAAATGTAATGATTGTATGGTCTTTAAGTTGATGCATCATTTTTATTTATTAATTATTGAAAATCTTGCAGGATACTAGTTGAGTGTTTGGATGATGTTGATGCGGAGTCACTTAAAAGTGCCGCAGAATATTTAATGGAAACATTAGCAGATCCAGCAGCTATTGTCCTGGGCTCATGTCCAGGTGAAGGGAAGGTTAGTCTAGTGGCTGCTTTTACTCCAGGGGTGGTTGGTCAGGGAATTCAGGCAGGTAAATTTATTGGACAGATAGCAAAATTATGTGGTGGTGGAGGAGGTGGAAGGTCCAACTTCGCTCAGGCTGGTGGGAGGAAGCCTGAAAATTTGGCTGGTGCCCTCGAGAAAGCTAAATCAGAGCTTATAGCTTATCTTTGTGAAAAAGGGAACTGA
Protein sequence

>MS.gene68723.t1

MNVTLAVAAAAGGFISFKQLHLRRSRRLLPFCTSSSQDNDNNNNNNNNNNNNNCVSGDSIRERFLSFYASRGHKVLPSASLVPDDPTVLLTIAGMLQFKPIFLGKLPRQVPRATTSQRCIRTNDINNVGHTSRHHTFFEMLGNFSFGDYFKKQAIQWAWDLSTIEFGLPPERLWISVYQDDHETFQLWSQQVGVPVDRIKRLGEEDNFWTSGVTGPCGPCSEIYYDFHPERGYADVDLGDDTRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQKAPNNYETDLILPIIEKASELANISYGISDDQTKRYLKIVGDHMRAIVFLISDGVVPSNVGRGYVVRRLIRRVVRTGRLLGVKGDGRGDPEGAFLPIIAQKVLELSTHIDADVKNRAPRILEELKREELRFVQTLERGEKLLEEKLVDAISSAERSGTVPCLAGEDVFILYDTYGFPMEITKEVAEERGVSIDMNGFDIEMEKQRRQSQAAHNTVKLAIGSGTNIADNVPDTEFIGYESLYCKAIVASLVVNGDPAVQVREGSNVEILLNKTPFYAESGGQIGDHGFLYISEGENQPKAVVEIIDVQKSFGNIFVHKGTVQKGVLEVGKQVEATVDVKLRQRAKVHHTATHLLQAALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLLDSELAEIELLINGWIEDAILLQTKVMPLADAKREGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVSNTSEIRGFKVISEQGIASGVRRIEAVAGEAFIEYVNARDLYLKQLCSTLKVKPEEVTVRIENLLEELRVARNENSAVRAKAAVYKASVIASRAFLVGHSKQYRILVECLDDVDAESLKSAAEYLMETLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRSNFAQAGGRKPENLAGALEKAKSELIAYLCEKGN