AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71604


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71604.t1 MTR_1g106075 98.485 132 1 1 1 132 1 131 1.41e-92 263
MS.gene71604.t1 MTR_3g008630 93.939 132 7 1 1 132 1 131 1.06e-87 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71604.t1 AT1G49975 62.687 134 43 3 1 132 1 129 5.90e-51 158

Find 35 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCACCACCAACATGGAATT+TGG 0.289275 1.4:+82080677 MS.gene71604:CDS
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1.4:+82080554 None:intergenic
GATTACTTTGAGTTCGTAGA+AGG 0.353863 1.4:+82081176 MS.gene71604:CDS
TATAGGGCAAACCATGTTAA+TGG 0.362210 1.4:+82080571 MS.gene71604:CDS
CAGAAAAGGGTATCCTTGAT+TGG 0.408398 1.4:+82081237 MS.gene71604:CDS
GTTTGCAAGGAAAGGAGGAG+GGG 0.424219 1.4:+82081201 MS.gene71604:CDS
GAAGCTTTCAGAAGCAGAAA+AGG 0.433331 1.4:+82081223 MS.gene71604:CDS
GGGTTTGCAAGGAAAGGAGG+AGG 0.434142 1.4:+82081199 MS.gene71604:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1.4:-82080681 None:intergenic
TTGGTGGTGCTTCTCAAAGT+TGG 0.441638 1.4:-82080665 None:intergenic
TGGTGGTGCTTCTCAAAGTT+GGG 0.449042 1.4:-82080664 None:intergenic
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1.4:-82080732 None:intergenic
TGTTAGCTTTAAGCCAATCA+AGG 0.469309 1.4:-82081250 None:intergenic
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1.4:+82080555 None:intergenic
AGAAGCAATGTCCTTGACTC+AGG 0.482163 1.4:-82080752 None:intergenic
GGTTTGCAAGGAAAGGAGGA+GGG 0.503240 1.4:+82081200 MS.gene71604:CDS
TTATTACAAGCGTAATTACA+AGG 0.507139 1.4:+82081154 MS.gene71604:CDS
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1.4:-82080684 None:intergenic
GTTAATGGCACTAACAGTTA+CGG 0.549686 1.4:+82080586 MS.gene71604:CDS
AACTGTTAGTGCCATTAACA+TGG 0.549756 1.4:-82080582 None:intergenic
GTATGCATCATCCAATGTGC+AGG 0.549946 1.4:+82080616 MS.gene71604:CDS
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1.4:-82080738 None:intergenic
TACTTTGAGTTCGTAGAAGG+GGG 0.574786 1.4:+82081179 MS.gene71604:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1.4:+82080686 MS.gene71604:CDS
ATTATGAACTGCCTGCACAT+TGG 0.588435 1.4:-82080627 None:intergenic
ATTACTTTGAGTTCGTAGAA+GGG 0.593520 1.4:+82081177 MS.gene71604:CDS
AAGCTTTCAGAAGCAGAAAA+GGG 0.600223 1.4:+82081224 MS.gene71604:CDS
GAAGAAAACAGAGGAAAACA+AGG 0.601206 1.4:+82081024 MS.gene71604:CDS
TTACTTTGAGTTCGTAGAAG+GGG 0.603502 1.4:+82081178 MS.gene71604:CDS
GAAGCAATGTCCTTGACTCA+GGG 0.617035 1.4:-82080751 None:intergenic
CCACCAACATGGAATTTGGA+AGG 0.619705 1.4:+82080681 MS.gene71604:CDS
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1.4:-82080720 None:intergenic
CAACGCAAGACCCTGAGTCA+AGG 0.623179 1.4:+82080741 MS.gene71604:CDS
TTTGAGAAGCACCACCAACA+TGG 0.627251 1.4:+82080670 MS.gene71604:CDS
AGAATATTTGAAGAAAACAG+AGG 0.670181 1.4:+82081015 MS.gene71604:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAATATTTGAAGAAAACAG+AGG + chr1.4:82081015-82081034 MS.gene71604:intron 25.0%
! TTATTACAAGCGTAATTACA+AGG + chr1.4:82081154-82081173 MS.gene71604:CDS 25.0%
AAAATGACAAAGAGGTAAGT+AGG + chr1.4:82081050-82081069 MS.gene71604:intron 30.0%
AAATGACAAAGAGGTAAGTA+GGG + chr1.4:82081051-82081070 MS.gene71604:intron 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG + chr1.4:82081177-82081196 MS.gene71604:CDS 30.0%
! TTAATCACAGTTTTTGTGTG+TGG + chr1.4:82081117-82081136 MS.gene71604:intron 30.0%
AAGCTTTCAGAAGCAGAAAA+GGG + chr1.4:82081224-82081243 MS.gene71604:CDS 35.0%
AATGACAAAGAGGTAAGTAG+GGG + chr1.4:82081052-82081071 MS.gene71604:intron 35.0%
CAAGGAGAAAAATGACAAAG+AGG + chr1.4:82081042-82081061 MS.gene71604:CDS 35.0%
GAAGAAAACAGAGGAAAACA+AGG + chr1.4:82081024-82081043 MS.gene71604:CDS 35.0%
GTTAATGGCACTAACAGTTA+CGG + chr1.4:82080586-82080605 MS.gene71604:CDS 35.0%
TAATCAAATGTGACTGTGTC+AGG - chr1.4:82080877-82080896 None:intergenic 35.0%
TATAGGGCAAACCATGTTAA+TGG + chr1.4:82080571-82080590 MS.gene71604:CDS 35.0%
! AACTGTTAGTGCCATTAACA+TGG - chr1.4:82080585-82080604 None:intergenic 35.0%
! GATTACTTTGAGTTCGTAGA+AGG + chr1.4:82081176-82081195 MS.gene71604:CDS 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG + chr1.4:82081178-82081197 MS.gene71604:CDS 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG - chr1.4:82081253-82081272 None:intergenic 35.0%
!!! CGACATGAACATGTTTTTAG+TGG + chr1.4:82080950-82080969 MS.gene71604:intron 35.0%
AACATGGAATTTGGAAGGAG+AGG + chr1.4:82080686-82080705 MS.gene71604:CDS 40.0%
ATTATGAACTGCCTGCACAT+TGG - chr1.4:82080630-82080649 None:intergenic 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG + chr1.4:82081223-82081242 MS.gene71604:CDS 40.0%
TCTCCTTCCAAATTCCATGT+TGG - chr1.4:82080687-82080706 None:intergenic 40.0%
! AGAAGTGATGACGCTACATA+GGG - chr1.4:82080823-82080842 None:intergenic 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG + chr1.4:82081179-82081198 MS.gene71604:CDS 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG + chr1.4:82081237-82081256 MS.gene71604:CDS 40.0%
AGAAGCAATGTCCTTGACTC+AGG - chr1.4:82080755-82080774 None:intergenic 45.0%
AGCACCACCAACATGGAATT+TGG + chr1.4:82080677-82080696 MS.gene71604:CDS 45.0%
CCACCAACATGGAATTTGGA+AGG + chr1.4:82080681-82080700 MS.gene71604:CDS 45.0%
CCTTCCAAATTCCATGTTGG+TGG - chr1.4:82080684-82080703 None:intergenic 45.0%
GAAGCAATGTCCTTGACTCA+GGG - chr1.4:82080754-82080773 None:intergenic 45.0%
GTATGCATCATCCAATGTGC+AGG + chr1.4:82080616-82080635 MS.gene71604:CDS 45.0%
TGACTGTGTCAGGTCAATGT+CGG - chr1.4:82080867-82080886 None:intergenic 45.0%
! ACAGTTTTTGTGTGTGGCAG+AGG + chr1.4:82081123-82081142 MS.gene71604:intron 45.0%
! CAGAAGTGATGACGCTACAT+AGG - chr1.4:82080824-82080843 None:intergenic 45.0%
! CAGTTTTTGTGTGTGGCAGA+GGG + chr1.4:82081124-82081143 MS.gene71604:intron 45.0%
! TGGTGGTGCTTCTCAAAGTT+GGG - chr1.4:82080667-82080686 None:intergenic 45.0%
!! TTGGTGGTGCTTCTCAAAGT+TGG - chr1.4:82080668-82080687 None:intergenic 45.0%
!! TTTGAGAAGCACCACCAACA+TGG + chr1.4:82080670-82080689 MS.gene71604:CDS 45.0%
AGAAGGGGGTTTGCAAGGAA+AGG + chr1.4:82081193-82081212 MS.gene71604:CDS 50.0%
GGTTTGCAAGGAAAGGAGGA+GGG + chr1.4:82081200-82081219 MS.gene71604:CDS 50.0%
GTTTGCAAGGAAAGGAGGAG+GGG + chr1.4:82081201-82081220 MS.gene71604:CDS 50.0%
TCAGCGTCGTGTTCTCTCAT+AGG + chr1.4:82080922-82080941 MS.gene71604:intron 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG + chr1.4:82081188-82081207 MS.gene71604:CDS 50.0%
AGGGGGTTTGCAAGGAAAGG+AGG + chr1.4:82081196-82081215 MS.gene71604:CDS 55.0%
CAACGCAAGACCCTGAGTCA+AGG + chr1.4:82080741-82080760 MS.gene71604:CDS 55.0%
GGGTTTGCAAGGAAAGGAGG+AGG + chr1.4:82081199-82081218 MS.gene71604:CDS 55.0%
TGACTCAGGGTCTTGCGTTG+CGG - chr1.4:82080741-82080760 None:intergenic 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG - chr1.4:82080735-82080754 None:intergenic 60.0%
TGCGGTTGCGGCGATAACAG+TGG - chr1.4:82080723-82080742 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 82080563 82081277 82080563 ID=MS.gene71604
chr1.4 mRNA 82080563 82081277 82080563 ID=MS.gene71604.t1;Parent=MS.gene71604
chr1.4 exon 82080563 82080779 82080563 ID=MS.gene71604.t1.exon1;Parent=MS.gene71604.t1
chr1.4 CDS 82080563 82080779 82080563 ID=cds.MS.gene71604.t1;Parent=MS.gene71604.t1
chr1.4 exon 82081017 82081063 82081017 ID=MS.gene71604.t1.exon2;Parent=MS.gene71604.t1
chr1.4 CDS 82081017 82081063 82081017 ID=cds.MS.gene71604.t1;Parent=MS.gene71604.t1
chr1.4 exon 82081143 82081277 82081143 ID=MS.gene71604.t1.exon3;Parent=MS.gene71604.t1
chr1.4 CDS 82081143 82081277 82081143 ID=cds.MS.gene71604.t1;Parent=MS.gene71604.t1
Gene Sequence

>MS.gene71604

ATGAGTTCTATAGGGCAAACCATGTTAATGGCACTAACAGTTACGGTAAACAAGTATGCATCATCCAATGTGCAGGCAGTTCATAATAGAAAACAAGCAAACCCAACTTTGAGAAGCACCACCAACATGGAATTTGGAAGGAGAGGACTTGTCTTATCCACTGTTATCGCCGCAACCGCAACGCAAGACCCTGAGTCAAGGACATTGCTTCTTCAAAGTATATATGCATAACTTTTTTTTGTACTTATTATGTCTTGCCCTATGTAGCGTCATCACTTCTGAATGTATGTGTGTCTCGTGTCCGACATTGACCTGACACAGTCACATTTGATTACATTCAATTAATTGTTAAATTATTATCAGCGTCGTGTTCTCTCATAGGTCATGCGACATGAACATGTTTTTAGTGGTTTAATTGCTAACTAAAAAAATGTTGTAATGAAAATGTTATCAGAATATTTGAAGAAAACAGAGGAAAACAAGGAGAAAAATGACAAAGAGGTAAGTAGGGGTATTTAATTTTATGTTGTAGTATGTCATATTTAGTATTGAATTTAATCACAGTTTTTGTGTGTGGCAGAGGGTTGATAGTTATTACAAGCGTAATTACAAGGATTACTTTGAGTTCGTAGAAGGGGGTTTGCAAGGAAAGGAGGAGGGGAAGCTTTCAGAAGCAGAAAAGGGTATCCTTGATTGGCTTAAAGCTAACAAGTGA

Protein sequence

>MS.gene71604.t1

MSSIGQTMLMALTVTVNKYASSNVQAVHNRKQANPTLRSTTNMEFGRRGLVLSTVIAATATQDPESRTLLLQKYLKKTEENKEKNDKERVDSYYKRNYKDYFEFVEGGLQGKEEGKLSEAEKGILDWLKANK