AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33248


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33248.t1 MTR_0050s0180 91.525 118 9 1 1 118 57 173 1.53e-73 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33248.t1 AT5G51720 75.610 82 18 1 37 118 28 107 6.02e-42 134

Find 34 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCACTCTTCCTTATATC+TGG 0.212325 6.3:+59680977 None:intergenic
CTGAACCATACCTGCAATAA+GGG 0.297458 6.3:+59680906 None:intergenic
ACTGAACCATACCTGCAATA+AGG 0.297703 6.3:+59680905 None:intergenic
AAAGGAACTAGTGTTGAAAT+TGG 0.341338 6.3:+59681069 None:intergenic
AACACTAGTTCCTTTGGTAT+TGG 0.371065 6.3:-59681061 MS.gene33248:CDS
AATTTCAACACTAGTTCCTT+TGG 0.378332 6.3:-59681067 MS.gene33248:CDS
TCTCACCTAAACGTGGCTTT+TGG 0.382823 6.3:+59681113 None:intergenic
TTGGTATTGGTAAGAAAGTA+AGG 0.397456 6.3:-59681048 MS.gene33248:CDS
TGGCTTTGTTGTGCTTTACA+TGG 0.398604 6.3:+59679795 None:intergenic
TATAAGGAAGAGTGAAGAAA+AGG 0.402584 6.3:-59680972 MS.gene33248:CDS
AGGCTGTTACCCCTTATTGC+AGG 0.413134 6.3:-59680916 MS.gene33248:intron
GTAGCATAAATCCAGATATA+AGG 0.421707 6.3:-59680988 MS.gene33248:CDS
GTGGTGGTGAAAGCAGAGGC+AGG 0.428721 6.3:-59681019 MS.gene33248:CDS
TTGTGCCAAAAGCCACGTTT+AGG 0.443126 6.3:-59681118 MS.gene33248:CDS
TGATACAGATGCTGGAGATC+AGG 0.452783 6.3:-59679842 MS.gene33248:intron
GGCACAAAACTACACCAACT+TGG 0.466620 6.3:+59681134 None:intergenic
GGTGGTGAAAGCAGAGGCAG+GGG 0.494390 6.3:-59681017 MS.gene33248:CDS
AAGCCACTGGAGATAATGTT+GGG 0.514803 6.3:-59679778 MS.gene33248:CDS
GCAATAAGGGGTAACAGCCT+TGG 0.526502 6.3:+59680919 None:intergenic
AAAGCCACTGGAGATAATGT+TGG 0.533290 6.3:-59679779 MS.gene33248:CDS
GAAAGTAAGGAGTGTGGTGG+TGG 0.540229 6.3:-59681035 MS.gene33248:CDS
TGGTGGTGAAAGCAGAGGCA+GGG 0.560277 6.3:-59681018 MS.gene33248:CDS
GGAACCTTTCCTCTGTGTGA+TGG 0.562888 6.3:-59679821 MS.gene33248:CDS
TACTTTCTTACCAATACCAA+AGG 0.570043 6.3:+59681051 None:intergenic
GTAAAGCACAACAAAGCCAC+TGG 0.579483 6.3:-59679791 MS.gene33248:CDS
TGTGCTAAGTGAAGTGTCCA+AGG 0.598167 6.3:-59680936 MS.gene33248:CDS
GCTTCCATCACACAGAGGAA+AGG 0.616730 6.3:+59679817 None:intergenic
AGGCCCAACATTATCTCCAG+TGG 0.629442 6.3:+59679775 None:intergenic
TGGTAAGAAAGTAAGGAGTG+TGG 0.636519 6.3:-59681041 MS.gene33248:CDS
TGAACCATACCTGCAATAAG+GGG 0.646965 6.3:+59680907 None:intergenic
GTGGTGAAAGCAGAGGCAGG+GGG 0.668326 6.3:-59681016 MS.gene33248:CDS
TGTGGTGGTGGTGAAAGCAG+AGG 0.723118 6.3:-59681023 MS.gene33248:CDS
TAAGAAAGTAAGGAGTGTGG+TGG 0.754978 6.3:-59681038 MS.gene33248:CDS
ACATGGCTTCCATCACACAG+AGG 0.761842 6.3:+59679812 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTTTATCATTTATTTA+TGG - chr6.3:59680029-59680048 MS.gene33248:intron 10.0%
!! CAATAATCAAATTTCAATAA+TGG + chr6.3:59680120-59680139 None:intergenic 15.0%
!! CATTATTGAAATTTGATTAT+TGG - chr6.3:59680118-59680137 MS.gene33248:intron 15.0%
!! AAAACAAATCTTAATCTTTC+TGG - chr6.3:59681031-59681050 MS.gene33248:CDS 20.0%
!! ATTTAGTTGAAAACAACTTA+TGG - chr6.3:59680192-59680211 MS.gene33248:intron 20.0%
!! CTAAATTCAATAAACTATCT+AGG + chr6.3:59680178-59680197 None:intergenic 20.0%
!! TATCACAAATTAACAATGTT+TGG - chr6.3:59680521-59680540 MS.gene33248:intron 20.0%
!! TATGCTTCTATAATATATGT+TGG - chr6.3:59680844-59680863 MS.gene33248:intron 20.0%
!!! AGAAAGATTAAGATTTGTTT+TGG + chr6.3:59681032-59681051 None:intergenic 20.0%
!!! TGATTATTGGTTAATTTATG+TGG - chr6.3:59680131-59680150 MS.gene33248:intron 20.0%
! CCACATAAAGAACTTATTAA+TGG - chr6.3:59680806-59680825 MS.gene33248:intron 25.0%
! CCATTAATAAGTTCTTTATG+TGG + chr6.3:59680809-59680828 None:intergenic 25.0%
! GAGAGAAAAAATGGAATTAT+AGG - chr6.3:59679814-59679833 MS.gene33248:CDS 25.0%
! TGATATTAGATGAATGATTC+TGG - chr6.3:59680368-59680387 MS.gene33248:intron 25.0%
!! ATGATAATCTGGAATAATGT+CGG - chr6.3:59680575-59680594 MS.gene33248:intron 25.0%
!! GCATTTTGTATATGATAATC+TGG - chr6.3:59680564-59680583 MS.gene33248:intron 25.0%
!!! AATCTTAATCTTTCTGGTTT+TGG - chr6.3:59681037-59681056 MS.gene33248:CDS 25.0%
!!! ATCTTAATCTTTCTGGTTTT+GGG - chr6.3:59681038-59681057 MS.gene33248:CDS 25.0%
AATTTCAACACTAGTTCCTT+TGG - chr6.3:59679841-59679860 MS.gene33248:intron 30.0%
GAATCTCTTGTTGAGAAATT+AGG - chr6.3:59680618-59680637 MS.gene33248:intron 30.0%
GCTTCTATAATATATGTTGG+TGG - chr6.3:59680847-59680866 MS.gene33248:intron 30.0%
GTAGCATAAATCCAGATATA+AGG - chr6.3:59679920-59679939 MS.gene33248:intron 30.0%
TACTTTCTTACCAATACCAA+AGG + chr6.3:59679860-59679879 None:intergenic 30.0%
TATAAGGAAGAGTGAAGAAA+AGG - chr6.3:59679936-59679955 MS.gene33248:intron 30.0%
TATGGTTAAGTGTATCATTG+TGG - chr6.3:59680047-59680066 MS.gene33248:intron 30.0%
TGAGAAATTAGGATGTTGAT+AGG - chr6.3:59680629-59680648 MS.gene33248:intron 30.0%
TGATTGATTACATCTGTTTC+TGG - chr6.3:59680446-59680465 MS.gene33248:intron 30.0%
TTATGTGGAATCTACTAACT+GGG + chr6.3:59680794-59680813 None:intergenic 30.0%
TTTATGTGGAATCTACTAAC+TGG + chr6.3:59680795-59680814 None:intergenic 30.0%
! AAAGGAACTAGTGTTGAAAT+TGG + chr6.3:59679842-59679861 None:intergenic 30.0%
! TGATAATCTGGAATAATGTC+GGG - chr6.3:59680576-59680595 MS.gene33248:intron 30.0%
!! TTGGTATTGGTAAGAAAGTA+AGG - chr6.3:59679860-59679879 MS.gene33248:intron 30.0%
AACACTAGTTCCTTTGGTAT+TGG - chr6.3:59679847-59679866 MS.gene33248:intron 35.0%
CGTTTAGGTGAGAGAAAAAA+TGG - chr6.3:59679805-59679824 MS.gene33248:CDS 35.0%
CTCTCAAATAGTTTCACAAG+TGG - chr6.3:59680241-59680260 MS.gene33248:intron 35.0%
TCTTCACTCTTCCTTATATC+TGG + chr6.3:59679934-59679953 None:intergenic 35.0%
TTTGTCGAATCTCTATAACG+TGG - chr6.3:59680751-59680770 MS.gene33248:intron 35.0%
! ACATAAGCAGGATTTTTACG+TGG + chr6.3:59680657-59680676 None:intergenic 35.0%
! GATAATCTGGAATAATGTCG+GGG - chr6.3:59680577-59680596 MS.gene33248:intron 35.0%
!! ATTACATCTGTTTCTGGAAG+TGG - chr6.3:59680452-59680471 MS.gene33248:intron 35.0%
!!! TTTTTTCTCTCACCTAAACG+TGG + chr6.3:59679805-59679824 None:intergenic 35.0%
AAAGCCACTGGAGATAATGT+TGG - chr6.3:59681129-59681148 MS.gene33248:CDS 40.0%
AAGCCACTGGAGATAATGTT+GGG - chr6.3:59681130-59681149 MS.gene33248:CDS 40.0%
ACTGAACCATACCTGCAATA+AGG + chr6.3:59680006-59680025 None:intergenic 40.0%
CTGAACCATACCTGCAATAA+GGG + chr6.3:59680005-59680024 None:intergenic 40.0%
TAAAAGGAGAGGGTTGTGTT+AGG - chr6.3:59680707-59680726 MS.gene33248:intron 40.0%
TAACGAGGCTTAACATAAGC+AGG + chr6.3:59680669-59680688 None:intergenic 40.0%
TAAGCCTCGTTATACATTCC+CGG - chr6.3:59680677-59680696 MS.gene33248:intron 40.0%
TGAACCATACCTGCAATAAG+GGG + chr6.3:59680004-59680023 None:intergenic 40.0%
TGGAATCTACTAACTGGGAT+CGG + chr6.3:59680789-59680808 None:intergenic 40.0%
TGGTAAGAAAGTAAGGAGTG+TGG - chr6.3:59679867-59679886 MS.gene33248:intron 40.0%
! ACAACCCTCTCCTTTTACTT+GGG + chr6.3:59680704-59680723 None:intergenic 40.0%
! TAAGAAAGTAAGGAGTGTGG+TGG - chr6.3:59679870-59679889 MS.gene33248:intron 40.0%
!! AAGTTTTACGTTGACTGTCG+AGG + chr6.3:59680733-59680752 None:intergenic 40.0%
!! GCATCTGTTTTAAGCCAAGT+TGG - chr6.3:59679760-59679779 MS.gene33248:CDS 40.0%
!! TGGCTTTGTTGTGCTTTACA+TGG + chr6.3:59681116-59681135 None:intergenic 40.0%
GGCACAAAACTACACCAACT+TGG + chr6.3:59679777-59679796 None:intergenic 45.0%
GTAAAGCACAACAAAGCCAC+TGG - chr6.3:59681117-59681136 MS.gene33248:CDS 45.0%
GTTACCCCTTATTGCAGGTA+TGG - chr6.3:59679997-59680016 MS.gene33248:intron 45.0%
TGATACAGATGCTGGAGATC+AGG - chr6.3:59681066-59681085 MS.gene33248:CDS 45.0%
TTGTGCCAAAAGCCACGTTT+AGG - chr6.3:59679790-59679809 MS.gene33248:CDS 45.0%
! CACAACCCTCTCCTTTTACT+TGG + chr6.3:59680705-59680724 None:intergenic 45.0%
! TCTCACCTAAACGTGGCTTT+TGG + chr6.3:59679798-59679817 None:intergenic 45.0%
! TGTGCTAAGTGAAGTGTCCA+AGG - chr6.3:59679972-59679991 MS.gene33248:intron 45.0%
ACATGGCTTCCATCACACAG+AGG + chr6.3:59681099-59681118 None:intergenic 50.0%
AGGCCCAACATTATCTCCAG+TGG + chr6.3:59681136-59681155 None:intergenic 50.0%
AGGCTGTTACCCCTTATTGC+AGG - chr6.3:59679992-59680011 MS.gene33248:intron 50.0%
CATTCCCGGTCCCAAGTAAA+AGG - chr6.3:59680691-59680710 MS.gene33248:intron 50.0%
CGGTCCCAAGTAAAAGGAGA+GGG - chr6.3:59680697-59680716 MS.gene33248:intron 50.0%
GCAATAAGGGGTAACAGCCT+TGG + chr6.3:59679992-59680011 None:intergenic 50.0%
GCTTCCATCACACAGAGGAA+AGG + chr6.3:59681094-59681113 None:intergenic 50.0%
GGAACCTTTCCTCTGTGTGA+TGG - chr6.3:59681087-59681106 MS.gene33248:CDS 50.0%
GGGTGCTTTGATACAGATGC+TGG - chr6.3:59681058-59681077 MS.gene33248:CDS 50.0%
! CCTCTCCTTTTACTTGGGAC+CGG + chr6.3:59680699-59680718 None:intergenic 50.0%
! CTCTCCTTTTACTTGGGACC+GGG + chr6.3:59680698-59680717 None:intergenic 50.0%
! GAAAGTAAGGAGTGTGGTGG+TGG - chr6.3:59679873-59679892 MS.gene33248:intron 50.0%
CCGGTCCCAAGTAAAAGGAG+AGG - chr6.3:59680696-59680715 MS.gene33248:intron 55.0%
GGGACCGGGAATGTATAACG+AGG + chr6.3:59680684-59680703 None:intergenic 55.0%
! TGGTGGTGAAAGCAGAGGCA+GGG - chr6.3:59679890-59679909 MS.gene33248:intron 55.0%
!! TGTGGTGGTGGTGAAAGCAG+AGG - chr6.3:59679885-59679904 MS.gene33248:intron 55.0%
GGTGGTGAAAGCAGAGGCAG+GGG - chr6.3:59679891-59679910 MS.gene33248:intron 60.0%
GTGGTGAAAGCAGAGGCAGG+GGG - chr6.3:59679892-59679911 MS.gene33248:intron 60.0%
! GTGGTGGTGAAAGCAGAGGC+AGG - chr6.3:59679889-59679908 MS.gene33248:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 59679757 59681173 59679757 ID=MS.gene33248
chr6.3 mRNA 59679757 59681173 59679757 ID=MS.gene33248.t1;Parent=MS.gene33248
chr6.3 exon 59680917 59681173 59680917 ID=MS.gene33248.t1.exon1;Parent=MS.gene33248.t1
chr6.3 CDS 59680917 59681173 59680917 ID=cds.MS.gene33248.t1;Parent=MS.gene33248.t1
chr6.3 exon 59679757 59679856 59679757 ID=MS.gene33248.t1.exon2;Parent=MS.gene33248.t1
chr6.3 CDS 59679757 59679856 59679757 ID=cds.MS.gene33248.t1;Parent=MS.gene33248.t1
Gene Sequence

>MS.gene33248

ATGGCATCTGTTTTAAGCCAAGTTGGTGTAGTTTTGTGCCAAAAGCCACGTTTAGGTGAGAGAAAAAATGGAATTATAGGTACCAATTTCAACACTAGTTCCTTTGGTATTGGTAAGAAAGTAAGGAGTGTGGTGGTGGTGAAAGCAGAGGCAGGGGGTGTGAGTAGCATAAATCCAGATATAAGGAAGAGTGAAGAAAAGGTTGTTGATTCTGTTGTGCTAAGTGAAGTGTCCAAGGCTGTTACCCCTTATTGCAGGTATGGTTCAGTTTTTTATGTTTATCATTTATTTATGGTTAAGTGTATCATTGTGGCTCTTAATTTATGTGATTTTAGTATGTTAAATTTTTAAAATCTTGATCCATTATTGAAATTTGATTATTGGTTAATTTATGTGGTTTTAGTGTTAGTGAAGCTATCCTAGATAGTTTATTGAATTTAGTTGAAAACAACTTATGGACAAGTCGTAGCTGTTTTCATAAGTTCTCTCAAATAGTTTCACAAGTGGCTATGTCGCACAAAGATCGAATCGAAAATCAAGAGCTAGCGTTGATATTATTAATAGTTGAGATCGAAATTATGAAACTCTAGTTTGTTATATTAGCATTTGCTTGATATTAGATGAATGATTCTGGTTTCTTTCAAGCTTGAAAACTAGTTAAGAATATACATTTCATGTCAAACTATGATTGATTGATTACATCTGTTTCTGGAAGTGGTTCAAAAAAAATGTCATTTGTGATGTTAATTTCGTTTCATCTATTTTATCACAAATTAACAATGTTTGGTTAAATTGTGTTAGTTGAATGCATTTTGTATATGATAATCTGGAATAATGTCGGGGTAGCATACAAAAGAGAGTGAATCTCTTGTTGAGAAATTAGGATGTTGATAGGTTCCACGTAAAAATCCTGCTTATGTTAAGCCTCGTTATACATTCCCGGTCCCAAGTAAAAGGAGAGGGTTGTGTTAGGCCTCGACAGTCAACGTAAAACTTTGTCGAATCTCTATAACGTGGATCAAATACGAGCCGATCCCAGTTAGTAGATTCCACATAAAGAACTTATTAATGGAAAGTTTCTTTATGATATGCTTCTATAATATATGTTGGTGGATGTAGTTCTAAATTTTATTATTATTATTATAATTACTCAAGAAAGCTTGTTCTTCATGTTTTGAAGTTATGATCTTAGCAAGTTAACGATGACATGAAAAGCAACATTTACATATTCTTTCTGCAATCTTCTTGAATATTAGTTTTCATGTTTAAATTCCAAAACAAATCTTAATCTTTCTGGTTTTGGGTGCTTTGATACAGATGCTGGAGATCAGGAACCTTTCCTCTGTGTGATGGAAGCCATGTAAAGCACAACAAAGCCACTGGAGATAATGTTGGGCCTCTTCTTTTGAAAAAATAG

Protein sequence

>MS.gene33248.t1

MASVLSQVGVVLCQKPRLGERKNGIIGTNFNTSSFGIGKKVRSVVVVKAEAGGVSSINPDIRKSEEKVVDSVVLSEVSKAVTPYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKK