AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86932


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86932.t1 MTR_1g106075 97.727 132 2 1 1 132 1 131 1.61e-91 261
MS.gene86932.t1 MTR_3g008630 93.182 132 8 1 1 132 1 131 1.06e-86 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86932.t1 AT1G49975 61.940 134 44 3 1 132 1 129 7.08e-50 155

Find 35 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCACCACCAACATGGAATT+TGG 0.289275 1.2:+79068009 MS.gene86932:CDS
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1.2:+79067886 None:intergenic
GATTACTTTGAGTTCGTAGA+AGG 0.353863 1.2:+79068517 MS.gene86932:CDS
CAGAAAAGGGTATCCTTGAT+TGG 0.408398 1.2:+79068578 MS.gene86932:CDS
GAAGCTTTCAGAAGCAGAAA+AGG 0.433331 1.2:+79068564 MS.gene86932:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1.2:-79068013 None:intergenic
TTGGTGGTGCTTCTCAAAGT+TGG 0.441638 1.2:-79067997 None:intergenic
GTTTGCAAGGAAAGGAAGAG+GGG 0.447267 1.2:+79068542 MS.gene86932:CDS
TGGTGGTGCTTCTCAAAGTT+GGG 0.449042 1.2:-79067996 None:intergenic
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1.2:-79068064 None:intergenic
GGGTTTGCAAGGAAAGGAAG+AGG 0.455002 1.2:+79068540 MS.gene86932:CDS
TGTTAGCTTTAAGCCAATCA+AGG 0.469309 1.2:-79068591 None:intergenic
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1.2:+79067887 None:intergenic
AGAAGCAATGTCCTTGACTC+AGG 0.482163 1.2:-79068084 None:intergenic
GGTTTGCAAGGAAAGGAAGA+GGG 0.496880 1.2:+79068541 MS.gene86932:CDS
TATAGGGCAAACCATGTTAA+CGG 0.498969 1.2:+79067903 MS.gene86932:CDS
TTATTACAAGCGTAATTACA+AGG 0.507139 1.2:+79068495 MS.gene86932:CDS
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1.2:-79068016 None:intergenic
AACTGTTAGTGCCGTTAACA+TGG 0.531678 1.2:-79067914 None:intergenic
GTATGCATCATCCAATGTGC+AGG 0.549946 1.2:+79067948 MS.gene86932:CDS
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1.2:-79068070 None:intergenic
TACTTTGAGTTCGTAGAAGG+GGG 0.574786 1.2:+79068520 MS.gene86932:CDS
GTTAACGGCACTAACAGTTA+CGG 0.575863 1.2:+79067918 MS.gene86932:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1.2:+79068018 MS.gene86932:CDS
ATTACTTTGAGTTCGTAGAA+GGG 0.593520 1.2:+79068518 MS.gene86932:CDS
AAGCTTTCAGAAGCAGAAAA+GGG 0.600223 1.2:+79068565 MS.gene86932:CDS
GAAGAAAACAGAGGAAAACA+AGG 0.601206 1.2:+79068366 MS.gene86932:CDS
TTACTTTGAGTTCGTAGAAG+GGG 0.603502 1.2:+79068519 MS.gene86932:CDS
ATTGTGAACTGCCTGCACAT+TGG 0.608070 1.2:-79067959 None:intergenic
GAAGCAATGTCCTTGACTCA+GGG 0.617035 1.2:-79068083 None:intergenic
CCACCAACATGGAATTTGGA+AGG 0.619705 1.2:+79068013 MS.gene86932:CDS
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1.2:-79068052 None:intergenic
CAACGCAAGACCCTGAGTCA+AGG 0.623179 1.2:+79068073 MS.gene86932:CDS
TTTGAGAAGCACCACCAACA+TGG 0.627251 1.2:+79068002 MS.gene86932:CDS
AGAATATTTGAAGAAAACAG+AGG 0.670181 1.2:+79068357 MS.gene86932:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAATATTTGAAGAAAACAG+AGG + chr1.2:79068357-79068376 MS.gene86932:intron 25.0%
! TTATTACAAGCGTAATTACA+AGG + chr1.2:79068495-79068514 MS.gene86932:CDS 25.0%
AAAATGACAAAGAGGTAAGT+AGG + chr1.2:79068392-79068411 MS.gene86932:intron 30.0%
AAATGACAAAGAGGTAAGTA+GGG + chr1.2:79068393-79068412 MS.gene86932:intron 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG + chr1.2:79068518-79068537 MS.gene86932:CDS 30.0%
!! TTTAATCACAGTTTTGTGTG+TGG + chr1.2:79068458-79068477 MS.gene86932:intron 30.0%
AAGCTTTCAGAAGCAGAAAA+GGG + chr1.2:79068565-79068584 MS.gene86932:CDS 35.0%
AATGACAAAGAGGTAAGTAG+GGG + chr1.2:79068394-79068413 MS.gene86932:intron 35.0%
CAAGGAGAAAAATGACAAAG+AGG + chr1.2:79068384-79068403 MS.gene86932:CDS 35.0%
GAAGAAAACAGAGGAAAACA+AGG + chr1.2:79068366-79068385 MS.gene86932:CDS 35.0%
TAATCAAATGTGACTGTGTC+AGG - chr1.2:79068206-79068225 None:intergenic 35.0%
TATAGGGCAAACCATGTTAA+CGG + chr1.2:79067903-79067922 MS.gene86932:CDS 35.0%
! GATTACTTTGAGTTCGTAGA+AGG + chr1.2:79068517-79068536 MS.gene86932:CDS 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG + chr1.2:79068519-79068538 MS.gene86932:CDS 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG - chr1.2:79068594-79068613 None:intergenic 35.0%
!!! CGACATGAACATGTTTTTAG+TGG + chr1.2:79068289-79068308 MS.gene86932:intron 35.0%
AACATGGAATTTGGAAGGAG+AGG + chr1.2:79068018-79068037 MS.gene86932:CDS 40.0%
CTTCTGAATATATGCGTGTC+TGG + chr1.2:79068167-79068186 MS.gene86932:intron 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG + chr1.2:79068564-79068583 MS.gene86932:CDS 40.0%
GTTAACGGCACTAACAGTTA+CGG + chr1.2:79067918-79067937 MS.gene86932:CDS 40.0%
TCTCCTTCCAAATTCCATGT+TGG - chr1.2:79068019-79068038 None:intergenic 40.0%
! AACTGTTAGTGCCGTTAACA+TGG - chr1.2:79067917-79067936 None:intergenic 40.0%
! AGAAGTGATGACGCTACATA+GGG - chr1.2:79068152-79068171 None:intergenic 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG + chr1.2:79068520-79068539 MS.gene86932:CDS 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG + chr1.2:79068578-79068597 MS.gene86932:CDS 40.0%
AGAAGCAATGTCCTTGACTC+AGG - chr1.2:79068087-79068106 None:intergenic 45.0%
AGCACCACCAACATGGAATT+TGG + chr1.2:79068009-79068028 MS.gene86932:CDS 45.0%
ATTGTGAACTGCCTGCACAT+TGG - chr1.2:79067962-79067981 None:intergenic 45.0%
CCACCAACATGGAATTTGGA+AGG + chr1.2:79068013-79068032 MS.gene86932:CDS 45.0%
CCTTCCAAATTCCATGTTGG+TGG - chr1.2:79068016-79068035 None:intergenic 45.0%
GAAGCAATGTCCTTGACTCA+GGG - chr1.2:79068086-79068105 None:intergenic 45.0%
GGTTTGCAAGGAAAGGAAGA+GGG + chr1.2:79068541-79068560 MS.gene86932:CDS 45.0%
GTATGCATCATCCAATGTGC+AGG + chr1.2:79067948-79067967 MS.gene86932:CDS 45.0%
GTTTGCAAGGAAAGGAAGAG+GGG + chr1.2:79068542-79068561 MS.gene86932:CDS 45.0%
TCAGCGTTGTGTTCTCTCAT+AGG + chr1.2:79068261-79068280 MS.gene86932:intron 45.0%
TGACTGTGTCAGGTCAATGT+CGG - chr1.2:79068196-79068215 None:intergenic 45.0%
! CAGAAGTGATGACGCTACAT+AGG - chr1.2:79068153-79068172 None:intergenic 45.0%
! TGGTGGTGCTTCTCAAAGTT+GGG - chr1.2:79067999-79068018 None:intergenic 45.0%
!! ACAGTTTTGTGTGTGGCAGA+GGG + chr1.2:79068465-79068484 MS.gene86932:intron 45.0%
!! TTGGTGGTGCTTCTCAAAGT+TGG - chr1.2:79068000-79068019 None:intergenic 45.0%
!! TTTGAGAAGCACCACCAACA+TGG + chr1.2:79068002-79068021 MS.gene86932:CDS 45.0%
AGAAGGGGGTTTGCAAGGAA+AGG + chr1.2:79068534-79068553 MS.gene86932:CDS 50.0%
GGGTTTGCAAGGAAAGGAAG+AGG + chr1.2:79068540-79068559 MS.gene86932:CDS 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG + chr1.2:79068529-79068548 MS.gene86932:CDS 50.0%
!! CACAGTTTTGTGTGTGGCAG+AGG + chr1.2:79068464-79068483 MS.gene86932:intron 50.0%
CAACGCAAGACCCTGAGTCA+AGG + chr1.2:79068073-79068092 MS.gene86932:CDS 55.0%
TGACTCAGGGTCTTGCGTTG+CGG - chr1.2:79068073-79068092 None:intergenic 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG - chr1.2:79068067-79068086 None:intergenic 60.0%
TGCGGTTGCGGCGATAACAG+TGG - chr1.2:79068055-79068074 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 79067895 79068618 79067895 ID=MS.gene86932
chr1.2 mRNA 79067895 79068618 79067895 ID=MS.gene86932.t1;Parent=MS.gene86932
chr1.2 exon 79067895 79068111 79067895 ID=MS.gene86932.t1.exon1;Parent=MS.gene86932.t1
chr1.2 CDS 79067895 79068111 79067895 ID=cds.MS.gene86932.t1;Parent=MS.gene86932.t1
chr1.2 exon 79068359 79068405 79068359 ID=MS.gene86932.t1.exon2;Parent=MS.gene86932.t1
chr1.2 CDS 79068359 79068405 79068359 ID=cds.MS.gene86932.t1;Parent=MS.gene86932.t1
chr1.2 exon 79068484 79068618 79068484 ID=MS.gene86932.t1.exon3;Parent=MS.gene86932.t1
chr1.2 CDS 79068484 79068618 79068484 ID=cds.MS.gene86932.t1;Parent=MS.gene86932.t1
Gene Sequence

>MS.gene86932

ATGAGTTCTATAGGGCAAACCATGTTAACGGCACTAACAGTTACGGTAAACAAGTATGCATCATCCAATGTGCAGGCAGTTCACAATAGAAAACAAGCAAACCCAACTTTGAGAAGCACCACCAACATGGAATTTGGAAGGAGAGGACTTGTCTTATCCACTGTTATCGCCGCAACCGCAACGCAAGACCCTGAGTCAAGGACATTGCTTCTTCAAAGTATATATGCATAACTCTTTTTTGTACTTATGTCTTGCCCTATGTAGCGTCATCACTTCTGAATATATGCGTGTCTGGTGTCCGACATTGACCTGACACAGTCACATTTGATTACATTCAATTATTTCAGTTTTTTTTTTGAATTATTATCAGCGTTGTGTTCTCTCATAGGTCATGCGACATGAACATGTTTTTAGTGGTTTAATTGCTAACTAAAAAAAATGTTGTAATGAAAATGTGTTATCAGAATATTTGAAGAAAACAGAGGAAAACAAGGAGAAAAATGACAAAGAGGTAAGTAGGGGTATTTAATTTTATGTTGTAGTATGTCATATTTAGTATTGAATTTAATCACAGTTTTGTGTGTGGCAGAGGGTTGATAGTTATTACAAGCGTAATTACAAGGATTACTTTGAGTTCGTAGAAGGGGGTTTGCAAGGAAAGGAAGAGGGGAAGCTTTCAGAAGCAGAAAAGGGTATCCTTGATTGGCTTAAAGCTAACAAGTGA

Protein sequence

>MS.gene86932.t1

MSSIGQTMLTALTVTVNKYASSNVQAVHNRKQANPTLRSTTNMEFGRRGLVLSTVIAATATQDPESRTLLLQKYLKKTEENKEKNDKERVDSYYKRNYKDYFEFVEGGLQGKEEGKLSEAEKGILDWLKANK