AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91325


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91325.t1 MTR_1g106075 97.727 132 2 1 1 132 1 131 1.03e-91 261
MS.gene91325.t1 MTR_3g008630 93.182 132 8 1 1 132 1 131 8.91e-87 249
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91325.t1 AT1G49975 61.940 134 44 3 1 132 1 129 3.75e-50 156

Find 35 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCACCACCAACATGGAATT+TGG 0.289275 1.3:+72641642 MS.gene91325:CDS
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1.3:+72641519 None:intergenic
GATTACTTTGAGTTCGTAGA+AGG 0.353863 1.3:+72642156 MS.gene91325:CDS
TATAGGGCAAACCATGTTAA+TGG 0.362210 1.3:+72641536 MS.gene91325:CDS
CAGAAAAGGGTATCCTTGAT+TGG 0.408398 1.3:+72642217 MS.gene91325:CDS
GAAGCTTTCAGAAGCAGAAA+AGG 0.433331 1.3:+72642203 MS.gene91325:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1.3:-72641646 None:intergenic
TTGGTGGTGCTTCTCAAAGT+TGG 0.441638 1.3:-72641630 None:intergenic
GTTTGCAAGGAAAGGAAGAG+GGG 0.447267 1.3:+72642181 MS.gene91325:CDS
TGGTGGTGCTTCTCAAAGTT+GGG 0.449042 1.3:-72641629 None:intergenic
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1.3:-72641697 None:intergenic
GGGTTTGCAAGGAAAGGAAG+AGG 0.455002 1.3:+72642179 MS.gene91325:CDS
TGTTAGCTTTAAGCCAATCA+AGG 0.469309 1.3:-72642230 None:intergenic
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1.3:+72641520 None:intergenic
AGAAGCAATGTCCTTGACTC+AGG 0.482163 1.3:-72641717 None:intergenic
GGTTTGCAAGGAAAGGAAGA+GGG 0.496880 1.3:+72642180 MS.gene91325:CDS
TTATTACAAGCGTAATTACA+AGG 0.507139 1.3:+72642134 MS.gene91325:CDS
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1.3:-72641649 None:intergenic
GTTAATGGCACTAACAGTTA+CGG 0.549686 1.3:+72641551 MS.gene91325:CDS
AACTGTTAGTGCCATTAACA+TGG 0.549756 1.3:-72641547 None:intergenic
TTATGCATCATCCAATGTGC+AGG 0.549946 1.3:+72641581 MS.gene91325:CDS
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1.3:-72641703 None:intergenic
TACTTTGAGTTCGTAGAAGG+GGG 0.574786 1.3:+72642159 MS.gene91325:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1.3:+72641651 MS.gene91325:CDS
ATTATGAACTGCCTGCACAT+TGG 0.588435 1.3:-72641592 None:intergenic
ATTACTTTGAGTTCGTAGAA+GGG 0.593520 1.3:+72642157 MS.gene91325:CDS
AAGCTTTCAGAAGCAGAAAA+GGG 0.600223 1.3:+72642204 MS.gene91325:CDS
GAAGAAAACAGAGGAAAACA+AGG 0.601206 1.3:+72642005 MS.gene91325:CDS
TTACTTTGAGTTCGTAGAAG+GGG 0.603502 1.3:+72642158 MS.gene91325:CDS
GAAGCAATGTCCTTGACTCA+GGG 0.617035 1.3:-72641716 None:intergenic
CCACCAACATGGAATTTGGA+AGG 0.619705 1.3:+72641646 MS.gene91325:CDS
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1.3:-72641685 None:intergenic
CAACGCAAGACCCTGAGTCA+AGG 0.623179 1.3:+72641706 MS.gene91325:CDS
TTTGAGAAGCACCACCAACA+TGG 0.627251 1.3:+72641635 MS.gene91325:CDS
AGAATATTTGAAGAAAACAG+AGG 0.670181 1.3:+72641996 MS.gene91325:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAATATTTGAAGAAAACAG+AGG + chr1.3:72641996-72642015 MS.gene91325:intron 25.0%
! TTATTACAAGCGTAATTACA+AGG + chr1.3:72642134-72642153 MS.gene91325:CDS 25.0%
AAAATGACAAAGAGGTAAGT+AGG + chr1.3:72642031-72642050 MS.gene91325:intron 30.0%
AAATGACAAAGAGGTAAGTA+GGG + chr1.3:72642032-72642051 MS.gene91325:intron 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG + chr1.3:72642157-72642176 MS.gene91325:CDS 30.0%
!! TTTAATCACAGTTTTGTGTG+TGG + chr1.3:72642097-72642116 MS.gene91325:intron 30.0%
AAGCTTTCAGAAGCAGAAAA+GGG + chr1.3:72642204-72642223 MS.gene91325:CDS 35.0%
AATGACAAAGAGGTAAGTAG+GGG + chr1.3:72642033-72642052 MS.gene91325:intron 35.0%
CAAGGAGAAAAATGACAAAG+AGG + chr1.3:72642023-72642042 MS.gene91325:CDS 35.0%
GAAGAAAACAGAGGAAAACA+AGG + chr1.3:72642005-72642024 MS.gene91325:CDS 35.0%
GTTAATGGCACTAACAGTTA+CGG + chr1.3:72641551-72641570 MS.gene91325:CDS 35.0%
TAATCAAATGTGACTGTGTC+AGG - chr1.3:72641839-72641858 None:intergenic 35.0%
TATAGGGCAAACCATGTTAA+TGG + chr1.3:72641536-72641555 MS.gene91325:CDS 35.0%
! AACTGTTAGTGCCATTAACA+TGG - chr1.3:72641550-72641569 None:intergenic 35.0%
! GATTACTTTGAGTTCGTAGA+AGG + chr1.3:72642156-72642175 MS.gene91325:CDS 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG + chr1.3:72642158-72642177 MS.gene91325:CDS 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG - chr1.3:72642233-72642252 None:intergenic 35.0%
!!! CGACATGAACATGTTTTTAG+TGG + chr1.3:72641928-72641947 MS.gene91325:intron 35.0%
AACATGGAATTTGGAAGGAG+AGG + chr1.3:72641651-72641670 MS.gene91325:CDS 40.0%
ATTATGAACTGCCTGCACAT+TGG - chr1.3:72641595-72641614 None:intergenic 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG + chr1.3:72642203-72642222 MS.gene91325:CDS 40.0%
TCTCCTTCCAAATTCCATGT+TGG - chr1.3:72641652-72641671 None:intergenic 40.0%
TTATGCATCATCCAATGTGC+AGG + chr1.3:72641581-72641600 MS.gene91325:CDS 40.0%
! AGAAGTGATGACGCTACATA+GGG - chr1.3:72641785-72641804 None:intergenic 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG + chr1.3:72642159-72642178 MS.gene91325:CDS 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG + chr1.3:72642217-72642236 MS.gene91325:CDS 40.0%
AGAAGCAATGTCCTTGACTC+AGG - chr1.3:72641720-72641739 None:intergenic 45.0%
AGCACCACCAACATGGAATT+TGG + chr1.3:72641642-72641661 MS.gene91325:CDS 45.0%
CCACCAACATGGAATTTGGA+AGG + chr1.3:72641646-72641665 MS.gene91325:CDS 45.0%
CCTTCCAAATTCCATGTTGG+TGG - chr1.3:72641649-72641668 None:intergenic 45.0%
CTTCTGAATGTATGCGTGTC+TGG + chr1.3:72641800-72641819 MS.gene91325:intron 45.0%
GAAGCAATGTCCTTGACTCA+GGG - chr1.3:72641719-72641738 None:intergenic 45.0%
GGTTTGCAAGGAAAGGAAGA+GGG + chr1.3:72642180-72642199 MS.gene91325:CDS 45.0%
GTTTGCAAGGAAAGGAAGAG+GGG + chr1.3:72642181-72642200 MS.gene91325:CDS 45.0%
TCAGCATCGTGTTCTCTCAT+AGG + chr1.3:72641900-72641919 MS.gene91325:intron 45.0%
TGACTGTGTCAGGTCAATGT+CGG - chr1.3:72641829-72641848 None:intergenic 45.0%
! CAGAAGTGATGACGCTACAT+AGG - chr1.3:72641786-72641805 None:intergenic 45.0%
! TGGTGGTGCTTCTCAAAGTT+GGG - chr1.3:72641632-72641651 None:intergenic 45.0%
!! ACAGTTTTGTGTGTGGCAGA+GGG + chr1.3:72642104-72642123 MS.gene91325:intron 45.0%
!! TTGGTGGTGCTTCTCAAAGT+TGG - chr1.3:72641633-72641652 None:intergenic 45.0%
!! TTTGAGAAGCACCACCAACA+TGG + chr1.3:72641635-72641654 MS.gene91325:CDS 45.0%
AGAAGGGGGTTTGCAAGGAA+AGG + chr1.3:72642173-72642192 MS.gene91325:CDS 50.0%
GGGTTTGCAAGGAAAGGAAG+AGG + chr1.3:72642179-72642198 MS.gene91325:CDS 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG + chr1.3:72642168-72642187 MS.gene91325:CDS 50.0%
!! CACAGTTTTGTGTGTGGCAG+AGG + chr1.3:72642103-72642122 MS.gene91325:intron 50.0%
CAACGCAAGACCCTGAGTCA+AGG + chr1.3:72641706-72641725 MS.gene91325:CDS 55.0%
TGACTCAGGGTCTTGCGTTG+CGG - chr1.3:72641706-72641725 None:intergenic 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG - chr1.3:72641700-72641719 None:intergenic 60.0%
TGCGGTTGCGGCGATAACAG+TGG - chr1.3:72641688-72641707 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 72641528 72642257 72641528 ID=MS.gene91325
chr1.3 mRNA 72641528 72642257 72641528 ID=MS.gene91325.t1;Parent=MS.gene91325
chr1.3 exon 72641528 72641744 72641528 ID=MS.gene91325.t1.exon1;Parent=MS.gene91325.t1
chr1.3 CDS 72641528 72641744 72641528 ID=cds.MS.gene91325.t1;Parent=MS.gene91325.t1
chr1.3 exon 72641998 72642044 72641998 ID=MS.gene91325.t1.exon2;Parent=MS.gene91325.t1
chr1.3 CDS 72641998 72642044 72641998 ID=cds.MS.gene91325.t1;Parent=MS.gene91325.t1
chr1.3 exon 72642123 72642257 72642123 ID=MS.gene91325.t1.exon3;Parent=MS.gene91325.t1
chr1.3 CDS 72642123 72642257 72642123 ID=cds.MS.gene91325.t1;Parent=MS.gene91325.t1
Gene Sequence

>MS.gene91325

ATGAGTTCTATAGGGCAAACCATGTTAATGGCACTAACAGTTACGGTAAACAATTATGCATCATCCAATGTGCAGGCAGTTCATAATAGAAAACAAGCAAACCCAACTTTGAGAAGCACCACCAACATGGAATTTGGAAGGAGAGGACTTGTCTTATCCACTGTTATCGCCGCAACCGCAACGCAAGACCCTGAGTCAAGGACATTGCTTCTTCAAAGTATATATGCATAACTCTTTTTTGTACTTATGTCTTGCCCTATGTAGCGTCATCACTTCTGAATGTATGCGTGTCTGGTGTCCGACATTGACCTGACACAGTCACATTTGATTACATTTAATTAATTCAGTTTTTTTTTTTTTTTTGAATTATTATCAGCATCGTGTTCTCTCATAGGTCATGCGACATGAACATGTTTTTAGTGGTTTAATTGCTAACTAAAAAAAATGTTGTAATGAAAATGTGTTATCAGAATATTTGAAGAAAACAGAGGAAAACAAGGAGAAAAATGACAAAGAGGTAAGTAGGGGTATTTAATTTTATGTTGTAGTATGTCATATTTAGTATTGAATTTAATCACAGTTTTGTGTGTGGCAGAGGGTTGATAGTTATTACAAGCGTAATTACAAGGATTACTTTGAGTTCGTAGAAGGGGGTTTGCAAGGAAAGGAAGAGGGGAAGCTTTCAGAAGCAGAAAAGGGTATCCTTGATTGGCTTAAAGCTAACAAGTGA

Protein sequence

>MS.gene91325.t1

MSSIGQTMLMALTVTVNNYASSNVQAVHNRKQANPTLRSTTNMEFGRRGLVLSTVIAATATQDPESRTLLLQKYLKKTEENKEKNDKERVDSYYKRNYKDYFEFVEGGLQGKEEGKLSEAEKGILDWLKANK