Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69798.t1 | XP_003596609.1 | 98.3 | 230 | 4 | 0 | 1 | 230 | 1 | 230 | 9.60E-120 | 439.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69798.t1 | Q9SGH4 | 55.4 | 224 | 94 | 3 | 1 | 223 | 1 | 219 | 6.6e-61 | 235.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69798.t1 | G7IHD6 | 98.3 | 230 | 4 | 0 | 1 | 230 | 1 | 230 | 6.9e-120 | 439.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene69798 | 0.896271 | 4.33E-76 | -1.69E-46 |
| MS.gene049351 | MS.gene69798 | 0.849664 | 2.80E-60 | -1.69E-46 |
| MS.gene049389 | MS.gene69798 | 0.81321 | 2.87E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene69798 | 0.815373 | 9.56E-52 | -1.69E-46 |
| MS.gene049491 | MS.gene69798 | 0.850065 | 2.16E-60 | -1.69E-46 |
| MS.gene049661 | MS.gene69798 | 0.871166 | 8.41E-67 | -1.69E-46 |
| MS.gene049662 | MS.gene69798 | 0.872059 | 4.25E-67 | -1.69E-46 |
| MS.gene049681 | MS.gene69798 | 0.800013 | 1.76E-48 | -1.69E-46 |
| MS.gene049758 | MS.gene69798 | 0.904801 | 8.40E-80 | -1.69E-46 |
| MS.gene049910 | MS.gene69798 | 0.835809 | 1.35E-56 | -1.69E-46 |
| MS.gene049915 | MS.gene69798 | 0.814642 | 1.39E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene69798 | 0.863243 | 2.86E-64 | -1.69E-46 |
| MS.gene050099 | MS.gene69798 | 0.85266 | 3.99E-61 | -1.69E-46 |
| MS.gene050128 | MS.gene69798 | 0.842492 | 2.50E-58 | -1.69E-46 |
| MS.gene050301 | MS.gene69798 | 0.91205 | 3.04E-83 | -1.69E-46 |
| MS.gene050397 | MS.gene69798 | 0.891143 | 5.20E-74 | -1.69E-46 |
| MS.gene050404 | MS.gene69798 | 0.866436 | 2.86E-65 | -1.69E-46 |
| MS.gene050406 | MS.gene69798 | 0.890061 | 1.38E-73 | -1.69E-46 |
| MS.gene050432 | MS.gene69798 | 0.865157 | 7.24E-65 | -1.69E-46 |
| MS.gene050464 | MS.gene69798 | 0.86998 | 2.06E-66 | -1.69E-46 |
| MS.gene050529 | MS.gene69798 | 0.868119 | 8.27E-66 | -1.69E-46 |
| MS.gene05053 | MS.gene69798 | 0.802409 | 5.69E-49 | -1.69E-46 |
| MS.gene050558 | MS.gene69798 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene69798 | 0.857085 | 2.08E-62 | -1.69E-46 |
| MS.gene050767 | MS.gene69798 | 0.832468 | 9.29E-56 | -1.69E-46 |
| MS.gene050770 | MS.gene69798 | 0.835165 | 1.97E-56 | -1.69E-46 |
| MS.gene050783 | MS.gene69798 | 0.81689 | 4.38E-52 | -1.69E-46 |
| MS.gene050812 | MS.gene69798 | 0.814742 | 1.32E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene69798 | 0.890516 | 9.18E-74 | -1.69E-46 |
| MS.gene05105 | MS.gene69798 | 0.855398 | 6.48E-62 | -1.69E-46 |
| MS.gene05119 | MS.gene69798 | -0.803157 | 3.99E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene69798 | 0.882642 | 8.78E-71 | -1.69E-46 |
| MS.gene051525 | MS.gene69798 | 0.805927 | 1.05E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene69798 | 0.866296 | 3.16E-65 | -1.69E-46 |
| MS.gene051662 | MS.gene69798 | 0.807245 | 5.55E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene69798 | 0.827496 | 1.52E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene69798 | 0.826748 | 2.29E-54 | -1.69E-46 |
| MS.gene051877 | MS.gene69798 | 0.815868 | 7.42E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene69798 | -0.807014 | 6.22E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene69798 | 0.840639 | 7.70E-58 | -1.69E-46 |
| MS.gene052078 | MS.gene69798 | 0.891668 | 3.22E-74 | -1.69E-46 |
| MS.gene052178 | MS.gene69798 | 0.859282 | 4.61E-63 | -1.69E-46 |
| MS.gene052188 | MS.gene69798 | 0.875346 | 3.31E-68 | -1.69E-46 |
| MS.gene052207 | MS.gene69798 | 0.818224 | 2.19E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene69798 | 0.863884 | 1.81E-64 | -1.69E-46 |
| MS.gene052290 | MS.gene69798 | 0.823072 | 1.69E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene69798 | 0.90434 | 1.36E-79 | -1.69E-46 |
| MS.gene052322 | MS.gene69798 | 0.83796 | 3.82E-57 | -1.69E-46 |
| MS.gene052326 | MS.gene69798 | 0.858405 | 8.43E-63 | -1.69E-46 |
| MS.gene052340 | MS.gene69798 | 0.901458 | 2.62E-78 | -1.69E-46 |
| MS.gene052421 | MS.gene69798 | 0.815823 | 7.59E-52 | -1.69E-46 |
| MS.gene052481 | MS.gene69798 | 0.814955 | 1.18E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene69798 | 0.853568 | 2.19E-61 | -1.69E-46 |
| MS.gene053073 | MS.gene69798 | 0.841349 | 5.01E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene69798 | 0.823816 | 1.13E-53 | -1.69E-46 |
| MS.gene053181 | MS.gene69798 | 0.810878 | 9.26E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene69798 | 0.882607 | 9.04E-71 | -1.69E-46 |
| MS.gene053322 | MS.gene69798 | 0.809037 | 2.31E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene69798 | 0.884798 | 1.41E-71 | -1.69E-46 |
| MS.gene053836 | MS.gene69798 | 0.900404 | 7.58E-78 | -1.69E-46 |
| MS.gene053907 | MS.gene69798 | 0.824874 | 6.37E-54 | -1.69E-46 |
| MS.gene053928 | MS.gene69798 | 0.860126 | 2.57E-63 | -1.69E-46 |
| MS.gene053971 | MS.gene69798 | 0.835955 | 1.24E-56 | -1.69E-46 |
| MS.gene054010 | MS.gene69798 | 0.807356 | 5.26E-50 | -1.69E-46 |
| MS.gene054011 | MS.gene69798 | 0.817667 | 2.93E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene69798 | 0.803228 | 3.85E-49 | -1.69E-46 |
| MS.gene054013 | MS.gene69798 | 0.803105 | 4.09E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene69798 | 0.861338 | 1.10E-63 | -1.69E-46 |
| MS.gene054183 | MS.gene69798 | 0.80083 | 1.20E-48 | -1.69E-46 |
| MS.gene054349 | MS.gene69798 | 0.840181 | 1.02E-57 | -1.69E-46 |
| MS.gene054376 | MS.gene69798 | 0.857947 | 1.15E-62 | -1.69E-46 |
| MS.gene054473 | MS.gene69798 | 0.846585 | 1.98E-59 | -1.69E-46 |
| MS.gene054542 | MS.gene69798 | 0.818227 | 2.19E-52 | -1.69E-46 |
| MS.gene05455 | MS.gene69798 | 0.850843 | 1.31E-60 | -1.69E-46 |
| MS.gene054618 | MS.gene69798 | 0.816849 | 4.47E-52 | -1.69E-46 |
| MS.gene054802 | MS.gene69798 | 0.83702 | 6.65E-57 | -1.69E-46 |
| MS.gene054803 | MS.gene69798 | 0.834514 | 2.87E-56 | -1.69E-46 |
| MS.gene054804 | MS.gene69798 | 0.839807 | 1.27E-57 | -1.69E-46 |
| MS.gene054918 | MS.gene69798 | 0.807597 | 4.68E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene69798 | 0.826332 | 2.87E-54 | -1.69E-46 |
| MS.gene054955 | MS.gene69798 | 0.800014 | 1.76E-48 | -1.69E-46 |
| MS.gene054983 | MS.gene69798 | 0.843642 | 1.24E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene69798 | 0.909943 | 3.25E-82 | -1.69E-46 |
| MS.gene055254 | MS.gene69798 | 0.837843 | 4.09E-57 | -1.69E-46 |
| MS.gene055277 | MS.gene69798 | 0.892792 | 1.14E-74 | -1.69E-46 |
| MS.gene055390 | MS.gene69798 | 0.858987 | 5.65E-63 | -1.69E-46 |
| MS.gene055397 | MS.gene69798 | 0.805618 | 1.22E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene69798 | 0.897139 | 1.88E-76 | -1.69E-46 |
| MS.gene055428 | MS.gene69798 | 0.893371 | 6.69E-75 | -1.69E-46 |
| MS.gene055499 | MS.gene69798 | 0.80316 | 3.98E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene69798 | 0.859427 | 4.17E-63 | -1.69E-46 |
| MS.gene055564 | MS.gene69798 | 0.807764 | 4.31E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene69798 | 0.831154 | 1.96E-55 | -1.69E-46 |
| MS.gene055712 | MS.gene69798 | 0.870861 | 1.06E-66 | -1.69E-46 |
| MS.gene055859 | MS.gene69798 | 0.806057 | 9.90E-50 | -1.69E-46 |
| MS.gene055896 | MS.gene69798 | 0.81938 | 1.20E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene69798 | 0.809039 | 2.30E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene69798 | 0.880575 | 4.90E-70 | -1.69E-46 |
| MS.gene056314 | MS.gene69798 | 0.803909 | 2.78E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene69798 | 0.878822 | 2.06E-69 | -1.69E-46 |
| MS.gene056410 | MS.gene69798 | 0.837796 | 4.21E-57 | -1.69E-46 |
| MS.gene056451 | MS.gene69798 | 0.839948 | 1.17E-57 | -1.69E-46 |
| MS.gene056609 | MS.gene69798 | 0.854027 | 1.62E-61 | -1.69E-46 |
| MS.gene056612 | MS.gene69798 | 0.888638 | 4.95E-73 | -1.69E-46 |
| MS.gene056659 | MS.gene69798 | 0.834402 | 3.06E-56 | -1.69E-46 |
| MS.gene056719 | MS.gene69798 | 0.802781 | 4.77E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene69798 | 0.804135 | 2.50E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene69798 | 0.809194 | 2.13E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene69798 | -0.823207 | 1.57E-53 | -1.69E-46 |
| MS.gene05702 | MS.gene69798 | 0.918393 | 1.65E-86 | -1.69E-46 |
| MS.gene057121 | MS.gene69798 | 0.851365 | 9.31E-61 | -1.69E-46 |
| MS.gene057123 | MS.gene69798 | 0.833884 | 4.13E-56 | -1.69E-46 |
| MS.gene057124 | MS.gene69798 | 0.819765 | 9.78E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene69798 | 0.811503 | 6.78E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene69798 | 0.837991 | 3.75E-57 | -1.69E-46 |
| MS.gene057295 | MS.gene69798 | 0.844776 | 6.13E-59 | -1.69E-46 |
| MS.gene05733 | MS.gene69798 | 0.86439 | 1.26E-64 | -1.69E-46 |
| MS.gene057396 | MS.gene69798 | 0.818102 | 2.34E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene69798 | 0.849243 | 3.66E-60 | -1.69E-46 |
| MS.gene057448 | MS.gene69798 | 0.858318 | 8.94E-63 | -1.69E-46 |
| MS.gene057449 | MS.gene69798 | 0.850938 | 1.23E-60 | -1.69E-46 |
| MS.gene057450 | MS.gene69798 | 0.86974 | 2.47E-66 | -1.69E-46 |
| MS.gene057749 | MS.gene69798 | 0.836373 | 9.72E-57 | -1.69E-46 |
| MS.gene057754 | MS.gene69798 | 0.826708 | 2.34E-54 | -1.69E-46 |
| MS.gene057779 | MS.gene69798 | 0.803997 | 2.67E-49 | -1.69E-46 |
| MS.gene057782 | MS.gene69798 | 0.83362 | 4.80E-56 | -1.69E-46 |
| MS.gene057796 | MS.gene69798 | 0.837465 | 5.12E-57 | -1.69E-46 |
| MS.gene057823 | MS.gene69798 | 0.827422 | 1.58E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene69798 | 0.913099 | 9.11E-84 | -1.69E-46 |
| MS.gene058006 | MS.gene69798 | 0.813657 | 2.29E-51 | -1.69E-46 |
| MS.gene058082 | MS.gene69798 | 0.844723 | 6.34E-59 | -1.69E-46 |
| MS.gene05811 | MS.gene69798 | 0.881768 | 1.82E-70 | -1.69E-46 |
| MS.gene058142 | MS.gene69798 | 0.84508 | 5.07E-59 | -1.69E-46 |
| MS.gene058233 | MS.gene69798 | 0.870978 | 9.70E-67 | -1.69E-46 |
| MS.gene058236 | MS.gene69798 | 0.809799 | 1.58E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene69798 | 0.854816 | 9.57E-62 | -1.69E-46 |
| MS.gene058293 | MS.gene69798 | 0.873418 | 1.49E-67 | -1.69E-46 |
| MS.gene058365 | MS.gene69798 | 0.848415 | 6.22E-60 | -1.69E-46 |
| MS.gene05842 | MS.gene69798 | 0.806799 | 6.90E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene69798 | 0.892087 | 2.19E-74 | -1.69E-46 |
| MS.gene058710 | MS.gene69798 | 0.816338 | 5.82E-52 | -1.69E-46 |
| MS.gene058749 | MS.gene69798 | 0.826912 | 2.09E-54 | -1.69E-46 |
| MS.gene058787 | MS.gene69798 | 0.848351 | 6.48E-60 | -1.69E-46 |
| MS.gene058845 | MS.gene69798 | 0.846101 | 2.68E-59 | -1.69E-46 |
| MS.gene05886 | MS.gene69798 | 0.84096 | 6.35E-58 | -1.69E-46 |
| MS.gene058928 | MS.gene69798 | 0.830953 | 2.20E-55 | -1.69E-46 |
| MS.gene059011 | MS.gene69798 | 0.817227 | 3.68E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene69798 | 0.901108 | 3.74E-78 | -1.69E-46 |
| MS.gene059228 | MS.gene69798 | 0.829324 | 5.49E-55 | -1.69E-46 |
| MS.gene059285 | MS.gene69798 | 0.821052 | 4.96E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene69798 | 0.83425 | 3.34E-56 | -1.69E-46 |
| MS.gene059326 | MS.gene69798 | 0.854462 | 1.21E-61 | -1.69E-46 |
| MS.gene059342 | MS.gene69798 | 0.801519 | 8.67E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene69798 | 0.827728 | 1.33E-54 | -1.69E-46 |
| MS.gene059518 | MS.gene69798 | 0.877401 | 6.47E-69 | -1.69E-46 |
| MS.gene059597 | MS.gene69798 | 0.882374 | 1.10E-70 | -1.69E-46 |
| MS.gene05959 | MS.gene69798 | 0.816586 | 5.12E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene69798 | 0.866867 | 2.08E-65 | -1.69E-46 |
| MS.gene059688 | MS.gene69798 | 0.901112 | 3.72E-78 | -1.69E-46 |
| MS.gene059692 | MS.gene69798 | 0.920489 | 1.20E-87 | -1.69E-46 |
| MS.gene059695 | MS.gene69798 | 0.918236 | 2.00E-86 | -1.69E-46 |
| MS.gene059708 | MS.gene69798 | 0.823637 | 1.24E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene69798 | 0.872011 | 4.41E-67 | -1.69E-46 |
| MS.gene059894 | MS.gene69798 | 0.91311 | 8.99E-84 | -1.69E-46 |
| MS.gene059899 | MS.gene69798 | 0.816419 | 5.58E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene69798 | 0.819661 | 1.03E-52 | -1.69E-46 |
| MS.gene059974 | MS.gene69798 | 0.828857 | 7.12E-55 | -1.69E-46 |
| MS.gene060005 | MS.gene69798 | 0.83027 | 3.23E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene69798 | 0.912719 | 1.41E-83 | -1.69E-46 |
| MS.gene060403 | MS.gene69798 | 0.84597 | 2.91E-59 | -1.69E-46 |
| MS.gene060407 | MS.gene69798 | 0.853982 | 1.67E-61 | -1.69E-46 |
| MS.gene060476 | MS.gene69798 | 0.833198 | 6.12E-56 | -1.69E-46 |
| MS.gene060495 | MS.gene69798 | 0.8177 | 2.88E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene69798 | 0.824994 | 5.97E-54 | -1.69E-46 |
| MS.gene06090 | MS.gene69798 | 0.802051 | 6.74E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene69798 | 0.935709 | 5.53E-97 | -1.69E-46 |
| MS.gene061051 | MS.gene69798 | -0.855782 | 5.01E-62 | -1.69E-46 |
| MS.gene061112 | MS.gene69798 | 0.850806 | 1.34E-60 | -1.69E-46 |
| MS.gene061154 | MS.gene69798 | 0.827615 | 1.42E-54 | -1.69E-46 |
| MS.gene061159 | MS.gene69798 | 0.827595 | 1.43E-54 | -1.69E-46 |
| MS.gene061200 | MS.gene69798 | 0.80412 | 2.52E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene69798 | 0.848153 | 7.35E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69798.t1 | MTR_2g082580 | 98.261 | 230 | 4 | 0 | 1 | 230 | 1 | 230 | 2.78e-170 | 468 |
| MS.gene69798.t1 | MTR_5g041910 | 29.358 | 109 | 77 | 0 | 104 | 212 | 141 | 249 | 1.37e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69798.t1 | AT3G01440 | 55.357 | 224 | 94 | 3 | 1 | 223 | 1 | 219 | 4.72e-83 | 247 |
| MS.gene69798.t1 | AT4G21280 | 25.664 | 226 | 161 | 4 | 1 | 223 | 1 | 222 | 1.19e-17 | 79.0 |
| MS.gene69798.t1 | AT4G21280 | 25.551 | 227 | 161 | 5 | 1 | 223 | 1 | 223 | 5.84e-17 | 77.0 |
| MS.gene69798.t1 | AT4G05180 | 28.249 | 177 | 123 | 2 | 50 | 223 | 54 | 229 | 2.92e-15 | 72.8 |
| MS.gene69798.t1 | AT4G05180 | 28.249 | 177 | 123 | 2 | 50 | 223 | 54 | 229 | 2.92e-15 | 72.8 |
| MS.gene69798.t1 | AT1G14150 | 29.032 | 124 | 88 | 0 | 98 | 221 | 64 | 187 | 4.03e-13 | 65.9 |
| MS.gene69798.t1 | AT1G14150 | 31.250 | 96 | 66 | 0 | 98 | 193 | 64 | 159 | 2.98e-11 | 60.5 |
Find 37 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTGCTTGTCCTGTCTTAA+AGG | 0.122519 | 2.4:-16606269 | None:intergenic |
| TAGCTAAGGATAATGGATTT+TGG | 0.177277 | 2.4:+16606419 | MS.gene69798:CDS |
| CTTGTTTCTCTAGTTCTTAC+AGG | 0.240422 | 2.4:+16606373 | MS.gene69798:CDS |
| TTAAAGGTTCTTTGTGCATT+TGG | 0.275452 | 2.4:-16606253 | None:intergenic |
| CAAGCAGTAGTTGGATTTCT+TGG | 0.280285 | 2.4:+16606283 | MS.gene69798:CDS |
| TTTGGAACAAGGCATGTAAT+TGG | 0.297323 | 2.4:-16606235 | None:intergenic |
| CTATTAGTGACAGTTGGTTC+TGG | 0.310416 | 2.4:-16606457 | None:intergenic |
| AAACTCTACATGGCAAATAT+TGG | 0.316818 | 2.4:+16607230 | MS.gene69798:CDS |
| GTGACAGTTGGTTCTGGAAT+AGG | 0.317646 | 2.4:-16606451 | None:intergenic |
| ATTTCACTAGCTAAGGATAA+TGG | 0.333626 | 2.4:+16606412 | MS.gene69798:CDS |
| TGAAAGTAGACTTGAGTCTT+AGG | 0.346421 | 2.4:-16607352 | None:intergenic |
| TGTAATTGGAGTTAAAGTTT+TGG | 0.348907 | 2.4:-16606221 | None:intergenic |
| GATCCTTAAAACACTTCCTT+TGG | 0.359342 | 2.4:-16607255 | None:intergenic |
| GATATTGCAAATGAGAAAAC+GGG | 0.400999 | 2.4:+16607185 | MS.gene69798:intron |
| TGAAACACAAGCATGCTATA+AGG | 0.409707 | 2.4:+16607804 | MS.gene69798:CDS |
| AATTGCTGCTTATCTTCAGC+TGG | 0.415937 | 2.4:-16607410 | None:intergenic |
| ACTCCATGCAAATTTGTTAG+AGG | 0.418986 | 2.4:-16606196 | None:intergenic |
| GACAGCTTGAAGAAGCTTCA+AGG | 0.421927 | 2.4:+16607767 | MS.gene69798:intron |
| AAGACACACTCAACTATGTT+AGG | 0.422431 | 2.4:+16607324 | MS.gene69798:CDS |
| AGATATTGCAAATGAGAAAA+CGG | 0.438542 | 2.4:+16607184 | MS.gene69798:intron |
| AAGACAGGACAAGCAGTAGT+TGG | 0.469412 | 2.4:+16606274 | MS.gene69798:CDS |
| ATCTTACTATTAGTGACAGT+TGG | 0.488173 | 2.4:-16606463 | None:intergenic |
| TTCTTTGTGCATTTGGAACA+AGG | 0.495526 | 2.4:-16606246 | None:intergenic |
| TGCATTTGATCTTCTTGCAA+TGG | 0.504301 | 2.4:+16607286 | MS.gene69798:CDS |
| TGAGAAGATTTCACTAGCTA+AGG | 0.522106 | 2.4:+16606405 | MS.gene69798:CDS |
| TGCTTAAAGATGTCATGGAC+AGG | 0.522204 | 2.4:+16607839 | MS.gene69798:CDS |
| ATTTGATCTTCTTGCAATGG+CGG | 0.551344 | 2.4:+16607289 | MS.gene69798:CDS |
| ATTGCTGCTTATCTTCAGCT+GGG | 0.551798 | 2.4:-16607409 | None:intergenic |
| ATGGCAAATATTGGAGCCAA+AGG | 0.555680 | 2.4:+16607239 | MS.gene69798:CDS |
| GCACAAAGAACCTTTAAGAC+AGG | 0.560597 | 2.4:+16606259 | MS.gene69798:CDS |
| TAAGCAGCAATTAACTGATG+TGG | 0.578245 | 2.4:+16607421 | MS.gene69798:CDS |
| AGTTATGCTTAAAGATGTCA+TGG | 0.580022 | 2.4:+16607834 | MS.gene69798:CDS |
| GCTCCTCTAACAAATTTGCA+TGG | 0.591211 | 2.4:+16606193 | MS.gene69798:CDS |
| TATCAAGAAGAAACTCTACA+TGG | 0.592066 | 2.4:+16607220 | MS.gene69798:CDS |
| ACACACTCAACTATGTTAGG+AGG | 0.598523 | 2.4:+16607327 | MS.gene69798:CDS |
| CTGTAAGAACTAGAGAAACA+AGG | 0.599334 | 2.4:-16606372 | None:intergenic |
| ATATTGCAAATGAGAAAACG+GGG | 0.743479 | 2.4:+16607186 | MS.gene69798:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATAATAAACAAATGTAA+TGG | - | chr2.4:16606828-16606847 | None:intergenic | 10.0% |
| !! | AAATATTAATTGTACTATCA+TGG | + | chr2.4:16606692-16606711 | MS.gene69798:intron | 15.0% |
| !! | ATATTCTATTATTCAAACTT+AGG | - | chr2.4:16607026-16607045 | None:intergenic | 15.0% |
| !! | ATTCACATAATATTAAATTG+CGG | - | chr2.4:16607710-16607729 | None:intergenic | 15.0% |
| !! | TATAGCTAATTAATTTACAT+AGG | + | chr2.4:16606496-16606515 | MS.gene69798:intron | 15.0% |
| !! | TTATTAATAAACAAATGCAA+TGG | + | chr2.4:16606739-16606758 | MS.gene69798:intron | 15.0% |
| !! | TTTGTTTATTATTAAATCGA+TGG | + | chr2.4:16606833-16606852 | MS.gene69798:intron | 15.0% |
| !!! | ATTGTTTGATAATTTTGAAA+AGG | + | chr2.4:16607451-16607470 | MS.gene69798:CDS | 15.0% |
| !! | AATATTATGTGAATAGATGT+TGG | + | chr2.4:16607716-16607735 | MS.gene69798:intron | 20.0% |
| !! | CAATGTAAATGATGATTATA+TGG | + | chr2.4:16607159-16607178 | MS.gene69798:intron | 20.0% |
| !! | TATAATTAACTTCATCATGA+TGG | + | chr2.4:16606857-16606876 | MS.gene69798:intron | 20.0% |
| !! | TTTATGTTTCAATTGACATT+TGG | + | chr2.4:16606587-16606606 | MS.gene69798:intron | 20.0% |
| ! | AGATATTGCAAATGAGAAAA+CGG | + | chr2.4:16607184-16607203 | MS.gene69798:intron | 25.0% |
| ! | GTTATGATTGAAATTTAGCA+TGG | + | chr2.4:16606660-16606679 | MS.gene69798:intron | 25.0% |
| ! | TATGTTATAGTCCAATTTAC+AGG | - | chr2.4:16606980-16606999 | None:intergenic | 25.0% |
| !! | ACATTTGGAACAATTATGAT+TGG | + | chr2.4:16606602-16606621 | MS.gene69798:intron | 25.0% |
| !! | CAATATTTTATCACAGAGTT+CGG | - | chr2.4:16607079-16607098 | None:intergenic | 25.0% |
| !! | TGTAATTGGAGTTAAAGTTT+TGG | - | chr2.4:16606224-16606243 | None:intergenic | 25.0% |
| !!! | ATCTGTTTACTTTTGAACTA+GGG | + | chr2.4:16607124-16607143 | MS.gene69798:intron | 25.0% |
| !!! | GTATGCTCTTTTTTAAATTG+CGG | + | chr2.4:16607473-16607492 | MS.gene69798:intron | 25.0% |
| !!! | TATCTGTTTACTTTTGAACT+AGG | + | chr2.4:16607123-16607142 | MS.gene69798:intron | 25.0% |
| AAACTCTACATGGCAAATAT+TGG | + | chr2.4:16607230-16607249 | MS.gene69798:CDS | 30.0% | |
| AGTTATGCTTAAAGATGTCA+TGG | + | chr2.4:16607834-16607853 | MS.gene69798:CDS | 30.0% | |
| ATATTGCAAATGAGAAAACG+GGG | + | chr2.4:16607186-16607205 | MS.gene69798:CDS | 30.0% | |
| ATCTTACTATTAGTGACAGT+TGG | - | chr2.4:16606466-16606485 | None:intergenic | 30.0% | |
| ATTTCACTAGCTAAGGATAA+TGG | + | chr2.4:16606412-16606431 | MS.gene69798:CDS | 30.0% | |
| CAAAATATTGCAGACAAATG+TGG | + | chr2.4:16607559-16607578 | MS.gene69798:intron | 30.0% | |
| CATAAAGTTGCAAACAAATG+TGG | + | chr2.4:16607632-16607651 | MS.gene69798:intron | 30.0% | |
| GATATTGCAAATGAGAAAAC+GGG | + | chr2.4:16607185-16607204 | MS.gene69798:intron | 30.0% | |
| TACCTATTAGTGAAGTACAA+TGG | + | chr2.4:16606780-16606799 | MS.gene69798:intron | 30.0% | |
| TATCAAGAAGAAACTCTACA+TGG | + | chr2.4:16607220-16607239 | MS.gene69798:CDS | 30.0% | |
| TTAAAGGTTCTTTGTGCATT+TGG | - | chr2.4:16606256-16606275 | None:intergenic | 30.0% | |
| ! | CACATTTGTCTGCAATATTT+TGG | - | chr2.4:16607561-16607580 | None:intergenic | 30.0% |
| !! | ATTTTAGTCCTTGATATTGC+AGG | + | chr2.4:16607510-16607529 | MS.gene69798:intron | 30.0% |
| !! | CATGTACTTTGATTTTGACA+AGG | + | chr2.4:16607373-16607392 | MS.gene69798:CDS | 30.0% |
| !!! | GATAATGGATTTTGGATTGA+TGG | + | chr2.4:16606427-16606446 | MS.gene69798:CDS | 30.0% |
| !!! | TAGCTAAGGATAATGGATTT+TGG | + | chr2.4:16606419-16606438 | MS.gene69798:CDS | 30.0% |
| AAGACACACTCAACTATGTT+AGG | + | chr2.4:16607324-16607343 | MS.gene69798:CDS | 35.0% | |
| ATTTGATCTTCTTGCAATGG+CGG | + | chr2.4:16607289-16607308 | MS.gene69798:CDS | 35.0% | |
| CTGTAAGAACTAGAGAAACA+AGG | - | chr2.4:16606375-16606394 | None:intergenic | 35.0% | |
| CTTGATATTGCAGGAAAAAG+TGG | + | chr2.4:16607519-16607538 | MS.gene69798:intron | 35.0% | |
| GATCCTTAAAACACTTCCTT+TGG | - | chr2.4:16607258-16607277 | None:intergenic | 35.0% | |
| TAAGCAGCAATTAACTGATG+TGG | + | chr2.4:16607421-16607440 | MS.gene69798:CDS | 35.0% | |
| TGAAACACAAGCATGCTATA+AGG | + | chr2.4:16607804-16607823 | MS.gene69798:CDS | 35.0% | |
| TGAAAGTAGACTTGAGTCTT+AGG | - | chr2.4:16607355-16607374 | None:intergenic | 35.0% | |
| TGAGAAGATTTCACTAGCTA+AGG | + | chr2.4:16606405-16606424 | MS.gene69798:CDS | 35.0% | |
| TGCATTTGATCTTCTTGCAA+TGG | + | chr2.4:16607286-16607305 | MS.gene69798:CDS | 35.0% | |
| TGCCATTGTACTTCACTAAT+AGG | - | chr2.4:16606785-16606804 | None:intergenic | 35.0% | |
| TTAGGGGTAATCCTGTAAAT+TGG | + | chr2.4:16606966-16606985 | MS.gene69798:intron | 35.0% | |
| ! | TTCTTTGTGCATTTGGAACA+AGG | - | chr2.4:16606249-16606268 | None:intergenic | 35.0% |
| ! | TTTGGAACAAGGCATGTAAT+TGG | - | chr2.4:16606238-16606257 | None:intergenic | 35.0% |
| !! | ACTCCATGCAAATTTGTTAG+AGG | - | chr2.4:16606199-16606218 | None:intergenic | 35.0% |
| !! | CTTGTTTCTCTAGTTCTTAC+AGG | + | chr2.4:16606373-16606392 | MS.gene69798:CDS | 35.0% |
| !!! | CACTTTTTCCTGCAATATCA+AGG | - | chr2.4:16607521-16607540 | None:intergenic | 35.0% |
| AATTGTGGCACAAATGTCGT+TGG | - | chr2.4:16607676-16607695 | None:intergenic | 40.0% | |
| ACACACTCAACTATGTTAGG+AGG | + | chr2.4:16607327-16607346 | MS.gene69798:CDS | 40.0% | |
| AGACCATACTAAGTGGCTTA+GGG | + | chr2.4:16606949-16606968 | MS.gene69798:intron | 40.0% | |
| ATGGCAAATATTGGAGCCAA+AGG | + | chr2.4:16607239-16607258 | MS.gene69798:CDS | 40.0% | |
| CAAGCAGTAGTTGGATTTCT+TGG | + | chr2.4:16606283-16606302 | MS.gene69798:CDS | 40.0% | |
| CATAATCATGACCGCAATTG+TGG | - | chr2.4:16607594-16607613 | None:intergenic | 40.0% | |
| GACTTGTGCACTTATCAGTA+AGG | - | chr2.4:16606916-16606935 | None:intergenic | 40.0% | |
| GCAAACAAATGTGGCTAATG+CGG | + | chr2.4:16607641-16607660 | MS.gene69798:intron | 40.0% | |
| GCTCCTCTAACAAATTTGCA+TGG | + | chr2.4:16606193-16606212 | MS.gene69798:CDS | 40.0% | |
| TACTGCTTGTCCTGTCTTAA+AGG | - | chr2.4:16606272-16606291 | None:intergenic | 40.0% | |
| TAGACCATACTAAGTGGCTT+AGG | + | chr2.4:16606948-16606967 | MS.gene69798:intron | 40.0% | |
| TGCTTAAAGATGTCATGGAC+AGG | + | chr2.4:16607839-16607858 | MS.gene69798:CDS | 40.0% | |
| TTGCGGTCATGATTATGTTG+TGG | + | chr2.4:16607597-16607616 | MS.gene69798:intron | 40.0% | |
| ! | AATTGCTGCTTATCTTCAGC+TGG | - | chr2.4:16607413-16607432 | None:intergenic | 40.0% |
| ! | ATTGCTGCTTATCTTCAGCT+GGG | - | chr2.4:16607412-16607431 | None:intergenic | 40.0% |
| ! | CTATTAGTGACAGTTGGTTC+TGG | - | chr2.4:16606460-16606479 | None:intergenic | 40.0% |
| !! | GCACAAAGAACCTTTAAGAC+AGG | + | chr2.4:16606259-16606278 | MS.gene69798:CDS | 40.0% |
| !!! | GAGCCAAAGGAAGTGTTTTA+AGG | + | chr2.4:16607252-16607271 | MS.gene69798:CDS | 40.0% |
| AAGACAGGACAAGCAGTAGT+TGG | + | chr2.4:16606274-16606293 | MS.gene69798:CDS | 45.0% | |
| GACAGCTTGAAGAAGCTTCA+AGG | + | chr2.4:16607767-16607786 | MS.gene69798:intron | 45.0% | |
| GACCATACTAAGTGGCTTAG+GGG | + | chr2.4:16606950-16606969 | MS.gene69798:intron | 45.0% | |
| GCTGACTAGACCATACTAAG+TGG | + | chr2.4:16606942-16606961 | MS.gene69798:intron | 45.0% | |
| GTCAGAGACGTAGACACAAT+TGG | + | chr2.4:16607053-16607072 | MS.gene69798:intron | 45.0% | |
| TACCCCTAAGCCACTTAGTA+TGG | - | chr2.4:16606955-16606974 | None:intergenic | 45.0% | |
| !! | GTGACAGTTGGTTCTGGAAT+AGG | - | chr2.4:16606454-16606473 | None:intergenic | 45.0% |
| ACAAATGTCGTTGGACCACG+CGG | - | chr2.4:16607667-16607686 | None:intergenic | 50.0% | |
| GCGGTCAACAACTGCAATTG+TGG | - | chr2.4:16607691-16607710 | None:intergenic | 50.0% | |
| GGCTGATGCGACCACAATTG+CGG | + | chr2.4:16607580-16607599 | MS.gene69798:intron | 55.0% | |
| ATGTGGCTAATGCGGCCGCG+TGG | + | chr2.4:16607649-16607668 | MS.gene69798:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 16606190 | 16607888 | 16606190 | ID=MS.gene69798 |
| chr2.4 | mRNA | 16606190 | 16607888 | 16606190 | ID=MS.gene69798.t1;Parent=MS.gene69798 |
| chr2.4 | exon | 16606190 | 16606478 | 16606190 | ID=MS.gene69798.t1.exon1;Parent=MS.gene69798.t1 |
| chr2.4 | CDS | 16606190 | 16606478 | 16606190 | ID=cds.MS.gene69798.t1;Parent=MS.gene69798.t1 |
| chr2.4 | exon | 16607186 | 16607472 | 16607186 | ID=MS.gene69798.t1.exon2;Parent=MS.gene69798.t1 |
| chr2.4 | CDS | 16607186 | 16607472 | 16607186 | ID=cds.MS.gene69798.t1;Parent=MS.gene69798.t1 |
| chr2.4 | exon | 16607772 | 16607888 | 16607772 | ID=MS.gene69798.t1.exon3;Parent=MS.gene69798.t1 |
| chr2.4 | CDS | 16607772 | 16607888 | 16607772 | ID=cds.MS.gene69798.t1;Parent=MS.gene69798.t1 |
| Gene Sequence |
| Protein sequence |
