AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91319


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91319.t1 MTR_1g106075 98.485 132 1 1 1 132 1 131 1.41e-92 263
MS.gene91319.t1 MTR_3g008630 93.939 132 7 1 1 132 1 131 1.06e-87 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91319.t1 AT1G49975 62.687 134 43 3 1 132 1 129 5.90e-51 158

Find 35 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCACCACCAACATGGAATT+TGG 0.289275 1.3:+72837465 MS.gene91319:CDS
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1.3:+72837342 None:intergenic
GATTACTTTGAGTTCGTAGA+AGG 0.353863 1.3:+72837979 MS.gene91319:CDS
TATAGGGCAAACCATGTTAA+TGG 0.362210 1.3:+72837359 MS.gene91319:CDS
CAGAAAAGGGTATCCTTGAT+TGG 0.408398 1.3:+72838040 MS.gene91319:CDS
GAAGCTTTCAGAAGCAGAAA+AGG 0.433331 1.3:+72838026 MS.gene91319:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1.3:-72837469 None:intergenic
TTGGTGGTGCTTCTCAAAGT+TGG 0.441638 1.3:-72837453 None:intergenic
GTTTGCAAGGAAAGGAAGAG+GGG 0.447267 1.3:+72838004 MS.gene91319:CDS
TGGTGGTGCTTCTCAAAGTT+GGG 0.449042 1.3:-72837452 None:intergenic
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1.3:-72837520 None:intergenic
GGGTTTGCAAGGAAAGGAAG+AGG 0.455002 1.3:+72838002 MS.gene91319:CDS
TGTTAGCTTTAAGCCAATCA+AGG 0.469309 1.3:-72838053 None:intergenic
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1.3:+72837343 None:intergenic
AGAAGCAATGTCCTTGACTC+AGG 0.482163 1.3:-72837540 None:intergenic
GGTTTGCAAGGAAAGGAAGA+GGG 0.496880 1.3:+72838003 MS.gene91319:CDS
TTATTACAAGCGTAATTACA+AGG 0.507139 1.3:+72837957 MS.gene91319:CDS
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1.3:-72837472 None:intergenic
GTTAATGGCACTAACAGTTA+CGG 0.549686 1.3:+72837374 MS.gene91319:CDS
AACTGTTAGTGCCATTAACA+TGG 0.549756 1.3:-72837370 None:intergenic
GTATGCATCATCCAATGTGC+AGG 0.549946 1.3:+72837404 MS.gene91319:CDS
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1.3:-72837526 None:intergenic
TACTTTGAGTTCGTAGAAGG+GGG 0.574786 1.3:+72837982 MS.gene91319:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1.3:+72837474 MS.gene91319:CDS
ATTATGAACTGCCTGCACAT+TGG 0.588435 1.3:-72837415 None:intergenic
ATTACTTTGAGTTCGTAGAA+GGG 0.593520 1.3:+72837980 MS.gene91319:CDS
AAGCTTTCAGAAGCAGAAAA+GGG 0.600223 1.3:+72838027 MS.gene91319:CDS
GAAGAAAACAGAGGAAAACA+AGG 0.601206 1.3:+72837828 MS.gene91319:CDS
TTACTTTGAGTTCGTAGAAG+GGG 0.603502 1.3:+72837981 MS.gene91319:CDS
GAAGCAATGTCCTTGACTCA+GGG 0.617035 1.3:-72837539 None:intergenic
CCACCAACATGGAATTTGGA+AGG 0.619705 1.3:+72837469 MS.gene91319:CDS
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1.3:-72837508 None:intergenic
CAACGCAAGACCCTGAGTCA+AGG 0.623179 1.3:+72837529 MS.gene91319:CDS
TTTGAGAAGCACCACCAACA+TGG 0.627251 1.3:+72837458 MS.gene91319:CDS
AGAATATTTGAAGAAAACAG+AGG 0.670181 1.3:+72837819 MS.gene91319:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAATATTTGAAGAAAACAG+AGG + chr1.3:72837819-72837838 MS.gene91319:intron 25.0%
! TTATTACAAGCGTAATTACA+AGG + chr1.3:72837957-72837976 MS.gene91319:CDS 25.0%
AAAATGACAAAGAGGTAAGT+AGG + chr1.3:72837854-72837873 MS.gene91319:intron 30.0%
AAATGACAAAGAGGTAAGTA+GGG + chr1.3:72837855-72837874 MS.gene91319:intron 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG + chr1.3:72837980-72837999 MS.gene91319:CDS 30.0%
!! TTTAATCACAGTTTTGTGTG+TGG + chr1.3:72837920-72837939 MS.gene91319:intron 30.0%
AAGCTTTCAGAAGCAGAAAA+GGG + chr1.3:72838027-72838046 MS.gene91319:CDS 35.0%
AATGACAAAGAGGTAAGTAG+GGG + chr1.3:72837856-72837875 MS.gene91319:intron 35.0%
CAAGGAGAAAAATGACAAAG+AGG + chr1.3:72837846-72837865 MS.gene91319:CDS 35.0%
GAAGAAAACAGAGGAAAACA+AGG + chr1.3:72837828-72837847 MS.gene91319:CDS 35.0%
GTTAATGGCACTAACAGTTA+CGG + chr1.3:72837374-72837393 MS.gene91319:CDS 35.0%
TAATCAAATGTGACTGTGTC+AGG - chr1.3:72837662-72837681 None:intergenic 35.0%
TATAGGGCAAACCATGTTAA+TGG + chr1.3:72837359-72837378 MS.gene91319:CDS 35.0%
! AACTGTTAGTGCCATTAACA+TGG - chr1.3:72837373-72837392 None:intergenic 35.0%
! GATTACTTTGAGTTCGTAGA+AGG + chr1.3:72837979-72837998 MS.gene91319:CDS 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG + chr1.3:72837981-72838000 MS.gene91319:CDS 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG - chr1.3:72838056-72838075 None:intergenic 35.0%
!!! CGACATGAACATGTTTTTAG+TGG + chr1.3:72837751-72837770 MS.gene91319:intron 35.0%
AACATGGAATTTGGAAGGAG+AGG + chr1.3:72837474-72837493 MS.gene91319:CDS 40.0%
ATTATGAACTGCCTGCACAT+TGG - chr1.3:72837418-72837437 None:intergenic 40.0%
CTTCTGAATGTATGTGTGTC+TGG + chr1.3:72837623-72837642 MS.gene91319:intron 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG + chr1.3:72838026-72838045 MS.gene91319:CDS 40.0%
TCTCCTTCCAAATTCCATGT+TGG - chr1.3:72837475-72837494 None:intergenic 40.0%
! AGAAGTGATGACGCTACATA+GGG - chr1.3:72837608-72837627 None:intergenic 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG + chr1.3:72837982-72838001 MS.gene91319:CDS 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG + chr1.3:72838040-72838059 MS.gene91319:CDS 40.0%
AGAAGCAATGTCCTTGACTC+AGG - chr1.3:72837543-72837562 None:intergenic 45.0%
AGCACCACCAACATGGAATT+TGG + chr1.3:72837465-72837484 MS.gene91319:CDS 45.0%
CCACCAACATGGAATTTGGA+AGG + chr1.3:72837469-72837488 MS.gene91319:CDS 45.0%
CCTTCCAAATTCCATGTTGG+TGG - chr1.3:72837472-72837491 None:intergenic 45.0%
GAAGCAATGTCCTTGACTCA+GGG - chr1.3:72837542-72837561 None:intergenic 45.0%
GGTTTGCAAGGAAAGGAAGA+GGG + chr1.3:72838003-72838022 MS.gene91319:CDS 45.0%
GTATGCATCATCCAATGTGC+AGG + chr1.3:72837404-72837423 MS.gene91319:CDS 45.0%
GTTTGCAAGGAAAGGAAGAG+GGG + chr1.3:72838004-72838023 MS.gene91319:CDS 45.0%
TCAGCATCGTGTTCTCTCAT+AGG + chr1.3:72837723-72837742 MS.gene91319:intron 45.0%
TGACTGTGTCAGGTCAATGT+CGG - chr1.3:72837652-72837671 None:intergenic 45.0%
! CAGAAGTGATGACGCTACAT+AGG - chr1.3:72837609-72837628 None:intergenic 45.0%
! TGGTGGTGCTTCTCAAAGTT+GGG - chr1.3:72837455-72837474 None:intergenic 45.0%
!! ACAGTTTTGTGTGTGGCAGA+GGG + chr1.3:72837927-72837946 MS.gene91319:intron 45.0%
!! TTGGTGGTGCTTCTCAAAGT+TGG - chr1.3:72837456-72837475 None:intergenic 45.0%
!! TTTGAGAAGCACCACCAACA+TGG + chr1.3:72837458-72837477 MS.gene91319:CDS 45.0%
AGAAGGGGGTTTGCAAGGAA+AGG + chr1.3:72837996-72838015 MS.gene91319:CDS 50.0%
GGGTTTGCAAGGAAAGGAAG+AGG + chr1.3:72838002-72838021 MS.gene91319:CDS 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG + chr1.3:72837991-72838010 MS.gene91319:CDS 50.0%
!! CACAGTTTTGTGTGTGGCAG+AGG + chr1.3:72837926-72837945 MS.gene91319:intron 50.0%
CAACGCAAGACCCTGAGTCA+AGG + chr1.3:72837529-72837548 MS.gene91319:CDS 55.0%
TGACTCAGGGTCTTGCGTTG+CGG - chr1.3:72837529-72837548 None:intergenic 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG - chr1.3:72837523-72837542 None:intergenic 60.0%
TGCGGTTGCGGCGATAACAG+TGG - chr1.3:72837511-72837530 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 72837351 72838080 72837351 ID=MS.gene91319
chr1.3 mRNA 72837351 72838080 72837351 ID=MS.gene91319.t1;Parent=MS.gene91319
chr1.3 exon 72837351 72837567 72837351 ID=MS.gene91319.t1.exon1;Parent=MS.gene91319.t1
chr1.3 CDS 72837351 72837567 72837351 ID=cds.MS.gene91319.t1;Parent=MS.gene91319.t1
chr1.3 exon 72837821 72837867 72837821 ID=MS.gene91319.t1.exon2;Parent=MS.gene91319.t1
chr1.3 CDS 72837821 72837867 72837821 ID=cds.MS.gene91319.t1;Parent=MS.gene91319.t1
chr1.3 exon 72837946 72838080 72837946 ID=MS.gene91319.t1.exon3;Parent=MS.gene91319.t1
chr1.3 CDS 72837946 72838080 72837946 ID=cds.MS.gene91319.t1;Parent=MS.gene91319.t1
Gene Sequence

>MS.gene91319

ATGAGTTCTATAGGGCAAACCATGTTAATGGCACTAACAGTTACGGTAAACAAGTATGCATCATCCAATGTGCAGGCAGTTCATAATAGAAAACAAGCAAACCCAACTTTGAGAAGCACCACCAACATGGAATTTGGAAGGAGAGGACTTGTCTTATCCACTGTTATCGCCGCAACCGCAACGCAAGACCCTGAGTCAAGGACATTGCTTCTTCAAAGTATATATGCATAACTCTTTTTTGTACTTATGTCTTGCCCTATGTAGCGTCATCACTTCTGAATGTATGTGTGTCTGGTGTCCGACATTGACCTGACACAGTCACATTTGATTACATTTAATTAATTCAGTTTTTTTTTTTTTTTTGAATTATTATCAGCATCGTGTTCTCTCATAGGTCATGCGACATGAACATGTTTTTAGTGGTTTAATTGCTAACTAAAAAAAATGTTGTAATGAAAATGTGTTATCAGAATATTTGAAGAAAACAGAGGAAAACAAGGAGAAAAATGACAAAGAGGTAAGTAGGGGTATTTAATTTTATGTTGTAGTATGTCATATTTAGTATTGAATTTAATCACAGTTTTGTGTGTGGCAGAGGGTTGATAGTTATTACAAGCGTAATTACAAGGATTACTTTGAGTTCGTAGAAGGGGGTTTGCAAGGAAAGGAAGAGGGGAAGCTTTCAGAAGCAGAAAAGGGTATCCTTGATTGGCTTAAAGCTAACAAGTGA

Protein sequence

>MS.gene91319.t1

MSSIGQTMLMALTVTVNKYASSNVQAVHNRKQANPTLRSTTNMEFGRRGLVLSTVIAATATQDPESRTLLLQKYLKKTEENKEKNDKERVDSYYKRNYKDYFEFVEGGLQGKEEGKLSEAEKGILDWLKANK