AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96705


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96705.t1 MTR_1g106075 98.485 132 1 1 1 132 1 131 1.41e-92 263
MS.gene96705.t1 MTR_3g008630 93.939 132 7 1 1 132 1 131 1.06e-87 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96705.t1 AT1G49975 62.687 134 43 3 1 132 1 129 5.90e-51 158

Find 35 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCACCACCAACATGGAATT+TGG 0.289275 1.1:-76218483 MS.gene96705:CDS
GAAGAGGCGATGAGTTCTAT+AGG 0.348824 1.1:-76218606 None:intergenic
GATTACTTTGAGTTCGTAGA+AGG 0.353863 1.1:-76217966 MS.gene96705:CDS
TATAGGGCAAACCATGTTAA+TGG 0.362210 1.1:-76218589 MS.gene96705:CDS
CAGAAAAGGGTATCCTTGAT+TGG 0.408398 1.1:-76217905 MS.gene96705:CDS
GAAGCTTTCAGAAGCAGAAA+AGG 0.433331 1.1:-76217919 MS.gene96705:CDS
CCTTCCAAATTCCATGTTGG+TGG 0.438290 1.1:+76218479 None:intergenic
TTGGTGGTGCTTCTCAAAGT+TGG 0.441638 1.1:+76218495 None:intergenic
GTTTGCAAGGAAAGGAAGAG+GGG 0.447267 1.1:-76217941 MS.gene96705:CDS
TGGTGGTGCTTCTCAAAGTT+GGG 0.449042 1.1:+76218496 None:intergenic
AGGGTCTTGCGTTGCGGTTG+CGG 0.451542 1.1:+76218428 None:intergenic
GGGTTTGCAAGGAAAGGAAG+AGG 0.455002 1.1:-76217943 MS.gene96705:CDS
TGTTAGCTTTAAGCCAATCA+AGG 0.469309 1.1:+76217892 None:intergenic
AAGAGGCGATGAGTTCTATA+GGG 0.479187 1.1:-76218605 None:intergenic
AGAAGCAATGTCCTTGACTC+AGG 0.482163 1.1:+76218408 None:intergenic
GGTTTGCAAGGAAAGGAAGA+GGG 0.496880 1.1:-76217942 MS.gene96705:CDS
TTATTACAAGCGTAATTACA+AGG 0.507139 1.1:-76217988 MS.gene96705:CDS
TCTCCTTCCAAATTCCATGT+TGG 0.526459 1.1:+76218476 None:intergenic
GTTAATGGCACTAACAGTTA+CGG 0.549686 1.1:-76218574 MS.gene96705:CDS
AACTGTTAGTGCCATTAACA+TGG 0.549756 1.1:+76218578 None:intergenic
GTATGCATCATCCAATGTGC+AGG 0.549946 1.1:-76218544 MS.gene96705:CDS
TGACTCAGGGTCTTGCGTTG+CGG 0.557938 1.1:+76218422 None:intergenic
TACTTTGAGTTCGTAGAAGG+GGG 0.574786 1.1:-76217963 MS.gene96705:CDS
AACATGGAATTTGGAAGGAG+AGG 0.586422 1.1:-76218474 MS.gene96705:CDS
ATTATGAACTGCCTGCACAT+TGG 0.588435 1.1:+76218533 None:intergenic
ATTACTTTGAGTTCGTAGAA+GGG 0.593520 1.1:-76217965 MS.gene96705:CDS
AAGCTTTCAGAAGCAGAAAA+GGG 0.600223 1.1:-76217918 MS.gene96705:CDS
GAAGAAAACAGAGGAAAACA+AGG 0.601206 1.1:-76218117 MS.gene96705:CDS
TTACTTTGAGTTCGTAGAAG+GGG 0.603502 1.1:-76217964 MS.gene96705:CDS
GAAGCAATGTCCTTGACTCA+GGG 0.617035 1.1:+76218409 None:intergenic
CCACCAACATGGAATTTGGA+AGG 0.619705 1.1:-76218479 MS.gene96705:CDS
TGCGGTTGCGGCGATAACAG+TGG 0.623047 1.1:+76218440 None:intergenic
CAACGCAAGACCCTGAGTCA+AGG 0.623179 1.1:-76218419 MS.gene96705:CDS
TTTGAGAAGCACCACCAACA+TGG 0.627251 1.1:-76218490 MS.gene96705:CDS
AGAATATTTGAAGAAAACAG+AGG 0.670181 1.1:-76218126 MS.gene96705:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAATATTTGAAGAAAACAG+AGG - chr1.1:76218358-76218377 MS.gene96705:intron 25.0%
! TTATTACAAGCGTAATTACA+AGG - chr1.1:76218496-76218515 MS.gene96705:CDS 25.0%
AAAATGACAAAGAGGTAAGT+AGG - chr1.1:76218393-76218412 MS.gene96705:intron 30.0%
AAATGACAAAGAGGTAAGTA+GGG - chr1.1:76218394-76218413 MS.gene96705:intron 30.0%
TAATCAAATGTGATTGTGTC+AGG + chr1.1:76218201-76218220 None:intergenic 30.0%
! ATTACTTTGAGTTCGTAGAA+GGG - chr1.1:76218519-76218538 MS.gene96705:CDS 30.0%
!! TTTAATCACAGTTTTGTGTG+TGG - chr1.1:76218459-76218478 MS.gene96705:CDS 30.0%
AAGAAGTGATGACACTACAT+AGG + chr1.1:76218148-76218167 None:intergenic 35.0%
AAGCTTTCAGAAGCAGAAAA+GGG - chr1.1:76218566-76218585 MS.gene96705:CDS 35.0%
AATGACAAAGAGGTAAGTAG+GGG - chr1.1:76218395-76218414 MS.gene96705:intron 35.0%
AGAAGTGATGACACTACATA+GGG + chr1.1:76218147-76218166 None:intergenic 35.0%
CAAGGAGAAAAATGACAAAG+AGG - chr1.1:76218385-76218404 MS.gene96705:intron 35.0%
CTTCTTAATGTATGCGTGTT+TGG - chr1.1:76218162-76218181 MS.gene96705:intron 35.0%
GAAGAAAACAGAGGAAAACA+AGG - chr1.1:76218367-76218386 MS.gene96705:intron 35.0%
GTTAATGGCACTAACAGTTA+CGG - chr1.1:76217910-76217929 MS.gene96705:CDS 35.0%
TATAGGGCAAACCATGTTAA+TGG - chr1.1:76217895-76217914 MS.gene96705:CDS 35.0%
! AACTGTTAGTGCCATTAACA+TGG + chr1.1:76217909-76217928 None:intergenic 35.0%
! GATTACTTTGAGTTCGTAGA+AGG - chr1.1:76218518-76218537 MS.gene96705:CDS 35.0%
! TTACTTTGAGTTCGTAGAAG+GGG - chr1.1:76218520-76218539 MS.gene96705:CDS 35.0%
!! TGTTAGCTTTAAGCCAATCA+AGG + chr1.1:76218595-76218614 None:intergenic 35.0%
!!! CGACATGAACATGTTTTTAG+TGG - chr1.1:76218289-76218308 MS.gene96705:intron 35.0%
AACATGGAATTTGGAAGGAG+AGG - chr1.1:76218010-76218029 MS.gene96705:intron 40.0%
ATTATGAACTGCCTGCACAT+TGG + chr1.1:76217954-76217973 None:intergenic 40.0%
GAAGCTTTCAGAAGCAGAAA+AGG - chr1.1:76218565-76218584 MS.gene96705:CDS 40.0%
TCTCCTTCCAAATTCCATGT+TGG + chr1.1:76218011-76218030 None:intergenic 40.0%
TGATTGTGTCAGGTCAATGT+CGG + chr1.1:76218191-76218210 None:intergenic 40.0%
! TACTTTGAGTTCGTAGAAGG+GGG - chr1.1:76218521-76218540 MS.gene96705:CDS 40.0%
!! CAGAAAAGGGTATCCTTGAT+TGG - chr1.1:76218579-76218598 MS.gene96705:CDS 40.0%
AGAAGCAATGTCCTTGACTC+AGG + chr1.1:76218079-76218098 None:intergenic 45.0%
AGCACCACCAACATGGAATT+TGG - chr1.1:76218001-76218020 MS.gene96705:CDS 45.0%
CCACCAACATGGAATTTGGA+AGG - chr1.1:76218005-76218024 MS.gene96705:intron 45.0%
CCTTCCAAATTCCATGTTGG+TGG + chr1.1:76218008-76218027 None:intergenic 45.0%
GAAGCAATGTCCTTGACTCA+GGG + chr1.1:76218078-76218097 None:intergenic 45.0%
GGTTTGCAAGGAAAGGAAGA+GGG - chr1.1:76218542-76218561 MS.gene96705:CDS 45.0%
GTATGCATCATCCAATGTGC+AGG - chr1.1:76217940-76217959 MS.gene96705:CDS 45.0%
GTTTGCAAGGAAAGGAAGAG+GGG - chr1.1:76218543-76218562 MS.gene96705:CDS 45.0%
! TGGTGGTGCTTCTCAAAGTT+GGG + chr1.1:76217991-76218010 None:intergenic 45.0%
!! ACAGTTTTGTGTGTGGCAGA+GGG - chr1.1:76218466-76218485 MS.gene96705:CDS 45.0%
!! TTGGTGGTGCTTCTCAAAGT+TGG + chr1.1:76217992-76218011 None:intergenic 45.0%
!! TTTGAGAAGCACCACCAACA+TGG - chr1.1:76217994-76218013 MS.gene96705:CDS 45.0%
AGAAGGGGGTTTGCAAGGAA+AGG - chr1.1:76218535-76218554 MS.gene96705:CDS 50.0%
GGGTTTGCAAGGAAAGGAAG+AGG - chr1.1:76218541-76218560 MS.gene96705:CDS 50.0%
TCAGCGTCGTGTTCTCTCAT+AGG - chr1.1:76218261-76218280 MS.gene96705:intron 50.0%
TTCGTAGAAGGGGGTTTGCA+AGG - chr1.1:76218530-76218549 MS.gene96705:CDS 50.0%
!! CACAGTTTTGTGTGTGGCAG+AGG - chr1.1:76218465-76218484 MS.gene96705:CDS 50.0%
CAACGCAAGACCCTGAGTCA+AGG - chr1.1:76218065-76218084 MS.gene96705:intron 55.0%
TGACTCAGGGTCTTGCGTTG+CGG + chr1.1:76218065-76218084 None:intergenic 55.0%
AGGGTCTTGCGTTGCGGTTG+CGG + chr1.1:76218059-76218078 None:intergenic 60.0%
TGCGGTTGCGGCGATAACAG+TGG + chr1.1:76218047-76218066 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 76217887 76218619 76217887 ID=MS.gene96705
chr1.1 mRNA 76217887 76218619 76217887 ID=MS.gene96705.t1;Parent=MS.gene96705
chr1.1 exon 76218403 76218619 76218403 ID=MS.gene96705.t1.exon1;Parent=MS.gene96705.t1
chr1.1 CDS 76218403 76218619 76218403 ID=cds.MS.gene96705.t1;Parent=MS.gene96705.t1
chr1.1 exon 76218100 76218146 76218100 ID=MS.gene96705.t1.exon2;Parent=MS.gene96705.t1
chr1.1 CDS 76218100 76218146 76218100 ID=cds.MS.gene96705.t1;Parent=MS.gene96705.t1
chr1.1 exon 76217887 76218021 76217887 ID=MS.gene96705.t1.exon3;Parent=MS.gene96705.t1
chr1.1 CDS 76217887 76218021 76217887 ID=cds.MS.gene96705.t1;Parent=MS.gene96705.t1
Gene Sequence

>MS.gene96705

ATGAGTTCTATAGGGCAAACCATGTTAATGGCACTAACAGTTACGGTAAACAAGTATGCATCATCCAATGTGCAGGCAGTTCATAATAGAAAACAAGCAAACCCAACTTTGAGAAGCACCACCAACATGGAATTTGGAAGGAGAGGACTTGTCTTATCCACTGTTATCGCCGCAACCGCAACGCAAGACCCTGAGTCAAGGACATTGCTTCTTCAAAGTATATATGCATAACTCTTTTTTGTACTTGTTATGTCTTGCCCTATGTAGTGTCATCACTTCTTAATGTATGCGTGTTTGGTGTCCGACATTGACCTGACACAATCACATTTGATTACATTCAATTAATTCAGTTTTTTTTTTTTTTTGAATTATTATCAGCGTCGTGTTCTCTCATAGGTCATGCGACATGAACATGTTTTTAGTGGTTTAATTGCTAACTGAAAAAAAATGTTGTAATGAAAATGTGTTATCAGAATATTTGAAGAAAACAGAGGAAAACAAGGAGAAAAATGACAAAGAGGTAAGTAGGGGTATTTAATTTTATGTTGTAGTATGTCATATTTAGTATTGAATTTAATCACAGTTTTGTGTGTGGCAGAGGGTTGATAGTTATTACAAGCGTAATTACAAGGATTACTTTGAGTTCGTAGAAGGGGGTTTGCAAGGAAAGGAAGAGGGGAAGCTTTCAGAAGCAGAAAAGGGTATCCTTGATTGGCTTAAAGCTAACAAGTGA

Protein sequence

>MS.gene96705.t1

MSSIGQTMLMALTVTVNKYASSNVQAVHNRKQANPTLRSTTNMEFGRRGLVLSTVIAATATQDPESRTLLLQKYLKKTEENKEKNDKERVDSYYKRNYKDYFEFVEGGLQGKEEGKLSEAEKGILDWLKANK