AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061679


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061679.t1 MTR_1g034260 99.213 127 1 0 1 127 1 127 4.05e-87 249
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061679.t1 AT2G26500 60.769 130 41 4 1 127 1 123 1.62e-43 139
MS.gene061679.t1 AT2G26500 60.769 130 41 4 1 127 1 123 1.62e-43 139
MS.gene061679.t1 AT2G26500 42.188 128 36 4 1 127 1 91 2.75e-21 82.0

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTCTTCCTCCATTTGA+TGG 0.243886 1.3:-19009058 None:intergenic
CTTCTTAGTACTCCTTGATT+TGG 0.303862 1.3:-19008900 None:intergenic
GTTGTAGGAAGCTCAGTTAT+TGG 0.363386 1.3:+19008875 MS.gene061679:CDS
CCATCTACAATCACTGTTGT+AGG 0.380548 1.3:+19008860 MS.gene061679:CDS
CAGATTGCAGCTATCATGAA+TGG 0.408360 1.3:+19009124 MS.gene061679:CDS
ACTCTTGTTGGTGTTGCTGT+TGG 0.440680 1.3:+19009151 MS.gene061679:CDS
AACAGCACTGTGACATCATT+AGG 0.444506 1.3:+19008980 MS.gene061679:CDS
CCTACAACAGTGATTGTAGA+TGG 0.469531 1.3:-19008860 None:intergenic
ATGAATGGACTTACTCTTGT+TGG 0.471407 1.3:+19009139 MS.gene061679:CDS
GGAGGAAGAAGAGGAGGTGC+AGG 0.478425 1.3:+19009067 MS.gene061679:CDS
CAAATCAAGGAGTACTAAGA+AGG 0.488070 1.3:+19008901 MS.gene061679:CDS
TCATTGAAATATCCATCAAA+TGG 0.499884 1.3:+19009046 MS.gene061679:CDS
ACAGGACTGAATTCATTCAA+TGG 0.502154 1.3:+19008944 MS.gene061679:CDS
CAGTTATTGGTACCAAATCA+AGG 0.536139 1.3:+19008888 MS.gene061679:CDS
TCTTCAACATGTAATGCTGC+AGG 0.547233 1.3:+19009091 MS.gene061679:CDS
CCATCAAATGGAGGAAGAAG+AGG 0.556781 1.3:+19009058 MS.gene061679:CDS
AAATCAAGGAGTACTAAGAA+GGG 0.580162 1.3:+19008902 MS.gene061679:CDS
AACAATGTGAAGTTTATCAC+AGG 0.583726 1.3:+19008926 MS.gene061679:CDS
TTGAAATATCCATCAAATGG+AGG 0.607875 1.3:+19009049 MS.gene061679:CDS
AAAGCTTGTTCAGTGCACAT+AGG 0.635805 1.3:-19009007 None:intergenic
TCAAATGGAGGAAGAAGAGG+AGG 0.645022 1.3:+19009061 MS.gene061679:CDS
TGTAGATGGAGTTGTTGCTG+AGG 0.669064 1.3:-19008846 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCATTGAAATATCCATCAAA+TGG + chr1.3:19009046-19009065 MS.gene061679:CDS 25.0%
AACAATGTGAAGTTTATCAC+AGG + chr1.3:19008926-19008945 MS.gene061679:CDS 30.0%
TTGAAATATCCATCAAATGG+AGG + chr1.3:19009049-19009068 MS.gene061679:CDS 30.0%
!! AAATCAAGGAGTACTAAGAA+GGG + chr1.3:19008902-19008921 MS.gene061679:CDS 30.0%
ACAGGACTGAATTCATTCAA+TGG + chr1.3:19008944-19008963 MS.gene061679:CDS 35.0%
CAGTTATTGGTACCAAATCA+AGG + chr1.3:19008888-19008907 MS.gene061679:CDS 35.0%
CTTCTTAGTACTCCTTGATT+TGG - chr1.3:19008903-19008922 None:intergenic 35.0%
! ATGAATGGACTTACTCTTGT+TGG + chr1.3:19009139-19009158 MS.gene061679:CDS 35.0%
!! CAAATCAAGGAGTACTAAGA+AGG + chr1.3:19008901-19008920 MS.gene061679:CDS 35.0%
AACAGCACTGTGACATCATT+AGG + chr1.3:19008980-19008999 MS.gene061679:CDS 40.0%
CAGATTGCAGCTATCATGAA+TGG + chr1.3:19009124-19009143 MS.gene061679:CDS 40.0%
CCATCTACAATCACTGTTGT+AGG + chr1.3:19008860-19008879 MS.gene061679:CDS 40.0%
CCTACAACAGTGATTGTAGA+TGG - chr1.3:19008863-19008882 None:intergenic 40.0%
GTTGTAGGAAGCTCAGTTAT+TGG + chr1.3:19008875-19008894 MS.gene061679:CDS 40.0%
TCTTCAACATGTAATGCTGC+AGG + chr1.3:19009091-19009110 MS.gene061679:CDS 40.0%
! AAAGCTTGTTCAGTGCACAT+AGG - chr1.3:19009010-19009029 None:intergenic 40.0%
CCATCAAATGGAGGAAGAAG+AGG + chr1.3:19009058-19009077 MS.gene061679:CDS 45.0%
CCTCTTCTTCCTCCATTTGA+TGG - chr1.3:19009061-19009080 None:intergenic 45.0%
TCAAATGGAGGAAGAAGAGG+AGG + chr1.3:19009061-19009080 MS.gene061679:CDS 45.0%
TGTAGATGGAGTTGTTGCTG+AGG - chr1.3:19008849-19008868 None:intergenic 45.0%
!! ACTCTTGTTGGTGTTGCTGT+TGG + chr1.3:19009151-19009170 MS.gene061679:CDS 45.0%
! GGAGGAAGAAGAGGAGGTGC+AGG + chr1.3:19009067-19009086 MS.gene061679:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 19008836 19009219 19008836 ID=MS.gene061679
chr1.3 mRNA 19008836 19009219 19008836 ID=MS.gene061679.t1;Parent=MS.gene061679
chr1.3 exon 19008836 19009219 19008836 ID=MS.gene061679.t1.exon1;Parent=MS.gene061679.t1
chr1.3 CDS 19008836 19009219 19008836 ID=cds.MS.gene061679.t1;Parent=MS.gene061679.t1
Gene Sequence

>MS.gene061679

ATGGCTACAGCCTCAGCAACAACTCCATCTACAATCACTGTTGTAGGAAGCTCAGTTATTGGTACCAAATCAAGGAGTACTAAGAAGGGTAACAATGTGAAGTTTATCACAGGACTGAATTCATTCAATGGATTGAAAGCACAGAACAGCACTGTGACATCATTAGGAGTTCCTATGTGCACTGAACAAGCTTTTGCAAAAGTGATGAGTTCATTGAAATATCCATCAAATGGAGGAAGAAGAGGAGGTGCAGGTTCTTCAACATGTAATGCTGCAGGTGAGATTTTTCAGATTGCAGCTATCATGAATGGACTTACTCTTGTTGGTGTTGCTGTTGGATTTGTTCTTCTTCGAATTGAAGCTGCTGTTGAAGAAGCTGAGTAA

Protein sequence

>MS.gene061679.t1

MATASATTPSTITVVGSSVIGTKSRSTKKGNNVKFITGLNSFNGLKAQNSTVTSLGVPMCTEQAFAKVMSSLKYPSNGGRRGGAGSSTCNAAGEIFQIAAIMNGLTLVGVAVGFVLLRIEAAVEEAE