Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25171.t1 | XP_013466593.1 | 94.8 | 172 | 9 | 0 | 1 | 172 | 1 | 172 | 8.60E-89 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25171.t1 | A0A072VF21 | 94.8 | 172 | 9 | 0 | 1 | 172 | 1 | 172 | 6.2e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene25171 | 0.816919 | 4.31E-52 | -1.69E-46 |
| MS.gene049284 | MS.gene25171 | 0.84519 | 4.74E-59 | -1.69E-46 |
| MS.gene049346 | MS.gene25171 | 0.809463 | 1.87E-50 | -1.69E-46 |
| MS.gene049351 | MS.gene25171 | 0.841375 | 4.94E-58 | -1.69E-46 |
| MS.gene049427 | MS.gene25171 | 0.88877 | 4.39E-73 | -1.69E-46 |
| MS.gene049491 | MS.gene25171 | 0.86622 | 3.34E-65 | -1.69E-46 |
| MS.gene049614 | MS.gene25171 | 0.837987 | 3.76E-57 | -1.69E-46 |
| MS.gene049661 | MS.gene25171 | 0.90461 | 1.03E-79 | -1.69E-46 |
| MS.gene049662 | MS.gene25171 | 0.899591 | 1.70E-77 | -1.69E-46 |
| MS.gene049758 | MS.gene25171 | 0.826561 | 2.54E-54 | -1.69E-46 |
| MS.gene049760 | MS.gene25171 | 0.835085 | 2.06E-56 | -1.69E-46 |
| MS.gene049805 | MS.gene25171 | 0.814541 | 1.46E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene25171 | 0.879774 | 9.47E-70 | -1.69E-46 |
| MS.gene050099 | MS.gene25171 | 0.804036 | 2.62E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene25171 | 0.877984 | 4.05E-69 | -1.69E-46 |
| MS.gene050397 | MS.gene25171 | 0.881736 | 1.87E-70 | -1.69E-46 |
| MS.gene050404 | MS.gene25171 | 0.875342 | 3.32E-68 | -1.69E-46 |
| MS.gene050406 | MS.gene25171 | 0.86326 | 2.83E-64 | -1.69E-46 |
| MS.gene050432 | MS.gene25171 | 0.85382 | 1.86E-61 | -1.69E-46 |
| MS.gene050464 | MS.gene25171 | 0.832859 | 7.43E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene25171 | 0.880174 | 6.82E-70 | -1.69E-46 |
| MS.gene050670 | MS.gene25171 | 0.833583 | 4.91E-56 | -1.69E-46 |
| MS.gene050767 | MS.gene25171 | 0.845272 | 4.50E-59 | -1.69E-46 |
| MS.gene050770 | MS.gene25171 | 0.849532 | 3.04E-60 | -1.69E-46 |
| MS.gene050783 | MS.gene25171 | 0.808138 | 3.59E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene25171 | 0.839806 | 1.27E-57 | -1.69E-46 |
| MS.gene05101 | MS.gene25171 | 0.891421 | 4.03E-74 | -1.69E-46 |
| MS.gene05105 | MS.gene25171 | 0.861507 | 9.78E-64 | -1.69E-46 |
| MS.gene051387 | MS.gene25171 | 0.80092 | 1.15E-48 | -1.69E-46 |
| MS.gene051471 | MS.gene25171 | 0.896496 | 3.49E-76 | -1.69E-46 |
| MS.gene051582 | MS.gene25171 | 0.830096 | 3.56E-55 | -1.69E-46 |
| MS.gene051605 | MS.gene25171 | 0.82492 | 6.22E-54 | -1.69E-46 |
| MS.gene051666 | MS.gene25171 | 0.823121 | 1.64E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene25171 | 0.801332 | 9.46E-49 | -1.69E-46 |
| MS.gene051877 | MS.gene25171 | 0.809922 | 1.49E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene25171 | 0.884769 | 1.44E-71 | -1.69E-46 |
| MS.gene052178 | MS.gene25171 | 0.846156 | 2.59E-59 | -1.69E-46 |
| MS.gene052188 | MS.gene25171 | 0.8605 | 1.98E-63 | -1.69E-46 |
| MS.gene052253 | MS.gene25171 | 0.864934 | 8.50E-65 | -1.69E-46 |
| MS.gene052290 | MS.gene25171 | 0.853648 | 2.08E-61 | -1.69E-46 |
| MS.gene052293 | MS.gene25171 | 0.805562 | 1.26E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene25171 | 0.910551 | 1.65E-82 | -1.69E-46 |
| MS.gene052322 | MS.gene25171 | 0.855177 | 7.51E-62 | -1.69E-46 |
| MS.gene052326 | MS.gene25171 | 0.866685 | 2.38E-65 | -1.69E-46 |
| MS.gene052340 | MS.gene25171 | 0.873663 | 1.23E-67 | -1.69E-46 |
| MS.gene052379 | MS.gene25171 | 0.812672 | 3.77E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene25171 | 0.827942 | 1.18E-54 | -1.69E-46 |
| MS.gene052481 | MS.gene25171 | 0.853042 | 3.10E-61 | -1.69E-46 |
| MS.gene052484 | MS.gene25171 | 0.833754 | 4.45E-56 | -1.69E-46 |
| MS.gene052485 | MS.gene25171 | 0.844424 | 7.63E-59 | -1.69E-46 |
| MS.gene053009 | MS.gene25171 | 0.807105 | 5.95E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene25171 | 0.854181 | 1.46E-61 | -1.69E-46 |
| MS.gene053104 | MS.gene25171 | 0.825674 | 4.12E-54 | -1.69E-46 |
| MS.gene053107 | MS.gene25171 | 0.801909 | 7.21E-49 | -1.69E-46 |
| MS.gene053181 | MS.gene25171 | 0.8156 | 8.51E-52 | -1.69E-46 |
| MS.gene053209 | MS.gene25171 | 0.842078 | 3.22E-58 | -1.69E-46 |
| MS.gene053322 | MS.gene25171 | 0.866773 | 2.23E-65 | -1.69E-46 |
| MS.gene053557 | MS.gene25171 | 0.857169 | 1.96E-62 | -1.69E-46 |
| MS.gene053758 | MS.gene25171 | 0.809386 | 1.94E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene25171 | 0.864622 | 1.06E-64 | -1.69E-46 |
| MS.gene053907 | MS.gene25171 | 0.805605 | 1.23E-49 | -1.69E-46 |
| MS.gene053971 | MS.gene25171 | 0.838454 | 2.85E-57 | -1.69E-46 |
| MS.gene054123 | MS.gene25171 | 0.850607 | 1.52E-60 | -1.69E-46 |
| MS.gene054376 | MS.gene25171 | 0.855966 | 4.42E-62 | -1.69E-46 |
| MS.gene05455 | MS.gene25171 | 0.828143 | 1.06E-54 | -1.69E-46 |
| MS.gene054802 | MS.gene25171 | 0.854442 | 1.23E-61 | -1.69E-46 |
| MS.gene054803 | MS.gene25171 | 0.855498 | 6.06E-62 | -1.69E-46 |
| MS.gene054804 | MS.gene25171 | 0.855001 | 8.46E-62 | -1.69E-46 |
| MS.gene054955 | MS.gene25171 | 0.817606 | 3.02E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene25171 | 0.807247 | 5.55E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene25171 | 0.82036 | 7.15E-53 | -1.69E-46 |
| MS.gene055116 | MS.gene25171 | 0.880706 | 4.40E-70 | -1.69E-46 |
| MS.gene055254 | MS.gene25171 | 0.832621 | 8.51E-56 | -1.69E-46 |
| MS.gene055277 | MS.gene25171 | 0.887982 | 8.84E-73 | -1.69E-46 |
| MS.gene055390 | MS.gene25171 | 0.838509 | 2.76E-57 | -1.69E-46 |
| MS.gene055397 | MS.gene25171 | 0.843789 | 1.13E-58 | -1.69E-46 |
| MS.gene055412 | MS.gene25171 | 0.846301 | 2.37E-59 | -1.69E-46 |
| MS.gene055422 | MS.gene25171 | 0.877102 | 8.22E-69 | -1.69E-46 |
| MS.gene055428 | MS.gene25171 | 0.88228 | 1.19E-70 | -1.69E-46 |
| MS.gene055499 | MS.gene25171 | 0.861748 | 8.25E-64 | -1.69E-46 |
| MS.gene055896 | MS.gene25171 | 0.857823 | 1.26E-62 | -1.69E-46 |
| MS.gene05592 | MS.gene25171 | 0.80316 | 3.98E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene25171 | 0.861154 | 1.25E-63 | -1.69E-46 |
| MS.gene056407 | MS.gene25171 | 0.838604 | 2.61E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene25171 | 0.825217 | 5.29E-54 | -1.69E-46 |
| MS.gene056451 | MS.gene25171 | 0.836579 | 8.62E-57 | -1.69E-46 |
| MS.gene056511 | MS.gene25171 | 0.809265 | 2.06E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene25171 | 0.864074 | 1.58E-64 | -1.69E-46 |
| MS.gene056612 | MS.gene25171 | 0.860444 | 2.06E-63 | -1.69E-46 |
| MS.gene056659 | MS.gene25171 | 0.826023 | 3.41E-54 | -1.69E-46 |
| MS.gene056796 | MS.gene25171 | 0.838748 | 2.39E-57 | -1.69E-46 |
| MS.gene056897 | MS.gene25171 | 0.828703 | 7.76E-55 | -1.69E-46 |
| MS.gene057011 | MS.gene25171 | 0.843089 | 1.74E-58 | -1.69E-46 |
| MS.gene05702 | MS.gene25171 | 0.894719 | 1.89E-75 | -1.69E-46 |
| MS.gene057121 | MS.gene25171 | 0.828737 | 7.61E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene25171 | 0.857742 | 1.33E-62 | -1.69E-46 |
| MS.gene057124 | MS.gene25171 | 0.807425 | 5.09E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene25171 | 0.850992 | 1.19E-60 | -1.69E-46 |
| MS.gene057146 | MS.gene25171 | 0.842767 | 2.12E-58 | -1.69E-46 |
| MS.gene057207 | MS.gene25171 | 0.80538 | 1.37E-49 | -1.69E-46 |
| MS.gene057245 | MS.gene25171 | 0.825885 | 3.67E-54 | -1.69E-46 |
| MS.gene057295 | MS.gene25171 | 0.850054 | 2.18E-60 | -1.69E-46 |
| MS.gene057310 | MS.gene25171 | 0.80142 | 9.08E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene25171 | 0.874608 | 5.91E-68 | -1.69E-46 |
| MS.gene057416 | MS.gene25171 | 0.804396 | 2.20E-49 | -1.69E-46 |
| MS.gene057449 | MS.gene25171 | 0.800441 | 1.44E-48 | -1.69E-46 |
| MS.gene057450 | MS.gene25171 | 0.804904 | 1.73E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene25171 | 0.825286 | 5.09E-54 | -1.69E-46 |
| MS.gene057782 | MS.gene25171 | 0.800473 | 1.42E-48 | -1.69E-46 |
| MS.gene057823 | MS.gene25171 | 0.813383 | 2.63E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene25171 | 0.860297 | 2.28E-63 | -1.69E-46 |
| MS.gene058019 | MS.gene25171 | 0.809934 | 1.48E-50 | -1.69E-46 |
| MS.gene058082 | MS.gene25171 | 0.843208 | 1.61E-58 | -1.69E-46 |
| MS.gene05811 | MS.gene25171 | 0.846678 | 1.87E-59 | -1.69E-46 |
| MS.gene058142 | MS.gene25171 | 0.806579 | 7.68E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene25171 | 0.90134 | 2.96E-78 | -1.69E-46 |
| MS.gene058276 | MS.gene25171 | 0.824725 | 6.91E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene25171 | 0.85062 | 1.51E-60 | -1.69E-46 |
| MS.gene058365 | MS.gene25171 | 0.829084 | 6.27E-55 | -1.69E-46 |
| MS.gene058376 | MS.gene25171 | 0.802135 | 6.48E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene25171 | 0.915072 | 9.08E-85 | -1.69E-46 |
| MS.gene058710 | MS.gene25171 | 0.800126 | 1.67E-48 | -1.69E-46 |
| MS.gene058787 | MS.gene25171 | 0.819977 | 8.75E-53 | -1.69E-46 |
| MS.gene058845 | MS.gene25171 | 0.82054 | 6.50E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene25171 | 0.864295 | 1.35E-64 | -1.69E-46 |
| MS.gene05907 | MS.gene25171 | 0.863967 | 1.70E-64 | -1.69E-46 |
| MS.gene059141 | MS.gene25171 | 0.828392 | 9.22E-55 | -1.69E-46 |
| MS.gene059285 | MS.gene25171 | 0.810972 | 8.83E-51 | -1.69E-46 |
| MS.gene059294 | MS.gene25171 | 0.813064 | 3.09E-51 | -1.69E-46 |
| MS.gene059326 | MS.gene25171 | 0.839783 | 1.29E-57 | -1.69E-46 |
| MS.gene059342 | MS.gene25171 | 0.805086 | 1.58E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene25171 | 0.802698 | 4.96E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene25171 | 0.824006 | 1.02E-53 | -1.69E-46 |
| MS.gene059536 | MS.gene25171 | 0.875296 | 3.45E-68 | -1.69E-46 |
| MS.gene059597 | MS.gene25171 | 0.885114 | 1.07E-71 | -1.69E-46 |
| MS.gene05959 | MS.gene25171 | 0.81293 | 3.31E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene25171 | 0.851559 | 8.20E-61 | -1.69E-46 |
| MS.gene059688 | MS.gene25171 | 0.898384 | 5.59E-77 | -1.69E-46 |
| MS.gene059692 | MS.gene25171 | 0.909014 | 9.09E-82 | -1.69E-46 |
| MS.gene059695 | MS.gene25171 | 0.905672 | 3.35E-80 | -1.69E-46 |
| MS.gene059708 | MS.gene25171 | 0.844256 | 8.46E-59 | -1.69E-46 |
| MS.gene05984 | MS.gene25171 | 0.8524 | 4.73E-61 | -1.69E-46 |
| MS.gene059858 | MS.gene25171 | 0.804362 | 2.24E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene25171 | 0.888445 | 5.87E-73 | -1.69E-46 |
| MS.gene059960 | MS.gene25171 | 0.868438 | 6.53E-66 | -1.69E-46 |
| MS.gene06037 | MS.gene25171 | 0.839105 | 1.93E-57 | -1.69E-46 |
| MS.gene060403 | MS.gene25171 | 0.825459 | 4.64E-54 | -1.69E-46 |
| MS.gene060407 | MS.gene25171 | 0.841181 | 5.55E-58 | -1.69E-46 |
| MS.gene060804 | MS.gene25171 | 0.859574 | 3.76E-63 | -1.69E-46 |
| MS.gene060818 | MS.gene25171 | 0.828016 | 1.14E-54 | -1.69E-46 |
| MS.gene06090 | MS.gene25171 | 0.814921 | 1.20E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene25171 | 0.840246 | 9.76E-58 | -1.69E-46 |
| MS.gene061049 | MS.gene25171 | 0.902576 | 8.42E-79 | -1.69E-46 |
| MS.gene061051 | MS.gene25171 | -0.842344 | 2.74E-58 | -1.69E-46 |
| MS.gene061200 | MS.gene25171 | 0.845456 | 4.02E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25171.t1 | MTR_1g032560 | 94.767 | 172 | 9 | 0 | 1 | 172 | 1 | 172 | 1.65e-113 | 320 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25171.t1 | AT2G01755 | 57.627 | 118 | 50 | 0 | 54 | 171 | 56 | 173 | 2.87e-42 | 139 |
| MS.gene25171.t1 | AT2G01755 | 57.627 | 118 | 50 | 0 | 54 | 171 | 62 | 179 | 3.82e-42 | 139 |
Find 51 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTCTATAGATAGAATAAA+AGG | 0.213144 | 1.3:-17458772 | MS.gene25171:CDS |
| ACCAAAGGCTTATACCTCTT+TGG | 0.304276 | 1.3:+17459283 | None:intergenic |
| TAAGTACTTCTACTTCATTT+TGG | 0.333138 | 1.3:+17459118 | None:intergenic |
| GGAAAGTTGTCAATTGATAA+TGG | 0.357903 | 1.3:+17459357 | None:intergenic |
| CCATGGCATCATTGTCCATT+TGG | 0.363669 | 1.3:+17459395 | None:intergenic |
| AGAATGCTTCTTGTTGGGTC+AGG | 0.383377 | 1.3:+17458814 | None:intergenic |
| GAAGATAGAGATGATGAGTA+TGG | 0.389990 | 1.3:-17459222 | MS.gene25171:CDS |
| GCTGGAAGAATGCTTCTTGT+TGG | 0.394629 | 1.3:+17458808 | None:intergenic |
| CTTCTGCAGATACTCAATAT+TGG | 0.406034 | 1.3:+17458735 | None:intergenic |
| CTGGAAGAATGCTTCTTGTT+GGG | 0.406470 | 1.3:+17458809 | None:intergenic |
| GACTTTATCACTGCCCTGGC+TGG | 0.412026 | 1.3:+17458790 | None:intergenic |
| TGTCCATTTGGCCATACCCT+TGG | 0.430122 | 1.3:+17459407 | None:intergenic |
| TCAATATTGGCAGGGTCAAA+AGG | 0.439860 | 1.3:+17458748 | None:intergenic |
| CAACGAGGAGATTTCAAAGT+TGG | 0.440151 | 1.3:-17459196 | MS.gene25171:CDS |
| GTCCATTTGGCCATACCCTT+GGG | 0.442034 | 1.3:+17459408 | None:intergenic |
| CTCTATCTTCATCCTCATCT+TGG | 0.448503 | 1.3:+17459235 | None:intergenic |
| TTGTCAATTGATAATGGTAT+AGG | 0.449082 | 1.3:+17459363 | None:intergenic |
| TGCAGATACTCAATATTGGC+AGG | 0.450870 | 1.3:+17458739 | None:intergenic |
| AGTAGAAGTACTTATCTTCA+AGG | 0.451782 | 1.3:-17459109 | MS.gene25171:intron |
| TTCCCAAGGGTATGGCCAAA+TGG | 0.469349 | 1.3:-17459410 | MS.gene25171:CDS |
| ACCAAAGAGGTATAAGCCTT+TGG | 0.474169 | 1.3:-17459284 | MS.gene25171:CDS |
| ATACCCTTGGGAAGTGTAGT+AGG | 0.478990 | 1.3:+17459420 | None:intergenic |
| GGACAATGATGCCATGGCCT+AGG | 0.481592 | 1.3:-17459389 | MS.gene25171:CDS |
| CAAGAAGCATTCTTCCAGCC+AGG | 0.483504 | 1.3:-17458804 | MS.gene25171:CDS |
| GAATGCTTCTTGTTGGGTCA+GGG | 0.497042 | 1.3:+17458815 | None:intergenic |
| CACAAGCTTACATTGTGAGT+TGG | 0.506810 | 1.3:+17459336 | None:intergenic |
| AGTCTATAGATAGAATAAAA+GGG | 0.523947 | 1.3:-17458771 | MS.gene25171:CDS |
| GCCTCCTTGCACCACACCAA+AGG | 0.530257 | 1.3:+17459268 | None:intergenic |
| AATGCTTCTTGTTGGGTCAG+GGG | 0.544161 | 1.3:+17458816 | None:intergenic |
| TACTACACTTCCCAAGGGTA+TGG | 0.545556 | 1.3:-17459418 | MS.gene25171:CDS |
| TTTGTCTTGAATTCTTCCCA+TGG | 0.554335 | 1.3:+17458706 | None:intergenic |
| ATCTGCAGAAGGATGTTCCA+TGG | 0.555118 | 1.3:-17458723 | MS.gene25171:CDS |
| GATAATGGTATAGGTAGCCT+AGG | 0.559854 | 1.3:+17459372 | None:intergenic |
| TATAGACTTTATCACTGCCC+TGG | 0.571801 | 1.3:+17458786 | None:intergenic |
| TAAGCCTTTGGTGTGGTGCA+AGG | 0.575947 | 1.3:-17459272 | MS.gene25171:CDS |
| ACTCATTGTTCATGCACTTG+TGG | 0.576626 | 1.3:-17459142 | MS.gene25171:CDS |
| GCATCCTACTACACTTCCCA+AGG | 0.594031 | 1.3:-17459424 | MS.gene25171:CDS |
| AAGAAGCATTCTTCCAGCCA+GGG | 0.602074 | 1.3:-17458803 | MS.gene25171:CDS |
| TTCTATAGTCAATCAGCAAG+AGG | 0.607447 | 1.3:-17459171 | MS.gene25171:CDS |
| AGGGGAAGTACCATAGCTCA+AGG | 0.608247 | 1.3:+17458834 | None:intergenic |
| TCTGCAGAAGGATGTTCCAT+GGG | 0.613447 | 1.3:-17458722 | MS.gene25171:CDS |
| CAATATTGAGTATCTGCAGA+AGG | 0.617485 | 1.3:-17458734 | MS.gene25171:CDS |
| CATCCTACTACACTTCCCAA+GGG | 0.625588 | 1.3:-17459423 | MS.gene25171:CDS |
| CCAAATGGACAATGATGCCA+TGG | 0.626699 | 1.3:-17459395 | MS.gene25171:CDS |
| GGTATAGGTAGCCTAGGCCA+TGG | 0.632885 | 1.3:+17459378 | None:intergenic |
| GCAAATTAGATACCAAGATG+AGG | 0.635629 | 1.3:-17459247 | MS.gene25171:CDS |
| GCAGATACTCAATATTGGCA+GGG | 0.648369 | 1.3:+17458740 | None:intergenic |
| TTAGATTTGAGCAACCAAAG+AGG | 0.657562 | 1.3:-17459297 | MS.gene25171:CDS |
| GCCTTTGGTGTGGTGCAAGG+AGG | 0.677190 | 1.3:-17459269 | MS.gene25171:CDS |
| AGAGGTATAAGCCTTTGGTG+TGG | 0.717085 | 1.3:-17459279 | MS.gene25171:CDS |
| TGATGAGTATGGACACAACG+AGG | 0.766111 | 1.3:-17459211 | MS.gene25171:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATTTGTTTGTTTTAT+TGG | + | chr1.3:17459086-17459105 | None:intergenic | 10.0% |
| !!! | TTTTTTTTCTAATTAGAAAA+AGG | - | chr1.3:17459128-17459147 | MS.gene25171:CDS | 10.0% |
| !! | AAGTCTATAGATAGAATAAA+AGG | - | chr1.3:17459344-17459363 | MS.gene25171:CDS | 20.0% |
| !! | AGTCTATAGATAGAATAAAA+GGG | - | chr1.3:17459345-17459364 | MS.gene25171:CDS | 20.0% |
| !!! | TTGTTTGTTTTATTGGTTAT+TGG | + | chr1.3:17459079-17459098 | None:intergenic | 20.0% |
| ! | CCATTTGCAAAACAAAAATT+TGG | + | chr1.3:17459185-17459204 | None:intergenic | 25.0% |
| !! | TAAGTACTTCTACTTCATTT+TGG | + | chr1.3:17459001-17459020 | None:intergenic | 25.0% |
| !!! | CCAAATTTTTGTTTTGCAAA+TGG | - | chr1.3:17459182-17459201 | MS.gene25171:CDS | 25.0% |
| !!! | TTGTCAATTGATAATGGTAT+AGG | + | chr1.3:17458756-17458775 | None:intergenic | 25.0% |
| ACTTATCTTCAAGGTAAGTT+TGG | - | chr1.3:17459016-17459035 | MS.gene25171:intron | 30.0% | |
| GGAAAGTTGTCAATTGATAA+TGG | + | chr1.3:17458762-17458781 | None:intergenic | 30.0% | |
| TGACAAACCATTTCATTTCA+GGG | - | chr1.3:17459248-17459267 | MS.gene25171:CDS | 30.0% | |
| !! | AGTAGAAGTACTTATCTTCA+AGG | - | chr1.3:17459007-17459026 | MS.gene25171:intron | 30.0% |
| AGAAAACCCCTGAAATGAAA+TGG | + | chr1.3:17459258-17459277 | None:intergenic | 35.0% | |
| CAATATTGAGTATCTGCAGA+AGG | - | chr1.3:17459382-17459401 | MS.gene25171:CDS | 35.0% | |
| CTTCTGCAGATACTCAATAT+TGG | + | chr1.3:17459384-17459403 | None:intergenic | 35.0% | |
| GAAGATAGAGATGATGAGTA+TGG | - | chr1.3:17458894-17458913 | MS.gene25171:intron | 35.0% | |
| GACAAACCATTTCATTTCAG+GGG | - | chr1.3:17459249-17459268 | MS.gene25171:CDS | 35.0% | |
| GCAAATTAGATACCAAGATG+AGG | - | chr1.3:17458869-17458888 | MS.gene25171:intron | 35.0% | |
| GTGACAAACCATTTCATTTC+AGG | - | chr1.3:17459247-17459266 | MS.gene25171:CDS | 35.0% | |
| TTAGATTTGAGCAACCAAAG+AGG | - | chr1.3:17458819-17458838 | MS.gene25171:CDS | 35.0% | |
| TTCTATAGTCAATCAGCAAG+AGG | - | chr1.3:17458945-17458964 | MS.gene25171:intron | 35.0% | |
| TTTGTCTTGAATTCTTCCCA+TGG | + | chr1.3:17459413-17459432 | None:intergenic | 35.0% | |
| ! | TTTTCTTCATCCTTGAGCTA+TGG | - | chr1.3:17459272-17459291 | MS.gene25171:CDS | 35.0% |
| ACCAAAGGCTTATACCTCTT+TGG | + | chr1.3:17458836-17458855 | None:intergenic | 40.0% | |
| ACTCATTGTTCATGCACTTG+TGG | - | chr1.3:17458974-17458993 | MS.gene25171:intron | 40.0% | |
| CAACGAGGAGATTTCAAAGT+TGG | - | chr1.3:17458920-17458939 | MS.gene25171:intron | 40.0% | |
| CACAAGCTTACATTGTGAGT+TGG | + | chr1.3:17458783-17458802 | None:intergenic | 40.0% | |
| CTCTATCTTCATCCTCATCT+TGG | + | chr1.3:17458884-17458903 | None:intergenic | 40.0% | |
| CTGGAAGAATGCTTCTTGTT+GGG | + | chr1.3:17459310-17459329 | None:intergenic | 40.0% | |
| GCAGATACTCAATATTGGCA+GGG | + | chr1.3:17459379-17459398 | None:intergenic | 40.0% | |
| TATAGACTTTATCACTGCCC+TGG | + | chr1.3:17459333-17459352 | None:intergenic | 40.0% | |
| TCAATATTGGCAGGGTCAAA+AGG | + | chr1.3:17459371-17459390 | None:intergenic | 40.0% | |
| TGCAGATACTCAATATTGGC+AGG | + | chr1.3:17459380-17459399 | None:intergenic | 40.0% | |
| ! | ACCAAAGAGGTATAAGCCTT+TGG | - | chr1.3:17458832-17458851 | MS.gene25171:CDS | 40.0% |
| ! | GATAATGGTATAGGTAGCCT+AGG | + | chr1.3:17458747-17458766 | None:intergenic | 40.0% |
| AATGCTTCTTGTTGGGTCAG+GGG | + | chr1.3:17459303-17459322 | None:intergenic | 45.0% | |
| AGAATGCTTCTTGTTGGGTC+AGG | + | chr1.3:17459305-17459324 | None:intergenic | 45.0% | |
| ATCTGCAGAAGGATGTTCCA+TGG | - | chr1.3:17459393-17459412 | MS.gene25171:CDS | 45.0% | |
| CATCCTACTACACTTCCCAA+GGG | - | chr1.3:17458693-17458712 | MS.gene25171:CDS | 45.0% | |
| CCAAATGGACAATGATGCCA+TGG | - | chr1.3:17458721-17458740 | MS.gene25171:CDS | 45.0% | |
| CCATGGCATCATTGTCCATT+TGG | + | chr1.3:17458724-17458743 | None:intergenic | 45.0% | |
| GAATGCTTCTTGTTGGGTCA+GGG | + | chr1.3:17459304-17459323 | None:intergenic | 45.0% | |
| GCTGGAAGAATGCTTCTTGT+TGG | + | chr1.3:17459311-17459330 | None:intergenic | 45.0% | |
| TACTACACTTCCCAAGGGTA+TGG | - | chr1.3:17458698-17458717 | MS.gene25171:CDS | 45.0% | |
| TCTGCAGAAGGATGTTCCAT+GGG | - | chr1.3:17459394-17459413 | MS.gene25171:CDS | 45.0% | |
| TGATGAGTATGGACACAACG+AGG | - | chr1.3:17458905-17458924 | MS.gene25171:intron | 45.0% | |
| ! | AGAGGTATAAGCCTTTGGTG+TGG | - | chr1.3:17458837-17458856 | MS.gene25171:CDS | 45.0% |
| ! | ATACCCTTGGGAAGTGTAGT+AGG | + | chr1.3:17458699-17458718 | None:intergenic | 45.0% |
| !! | AAGAAGCATTCTTCCAGCCA+GGG | - | chr1.3:17459313-17459332 | MS.gene25171:CDS | 45.0% |
| GCATCCTACTACACTTCCCA+AGG | - | chr1.3:17458692-17458711 | MS.gene25171:CDS | 50.0% | |
| GTCCATTTGGCCATACCCTT+GGG | + | chr1.3:17458711-17458730 | None:intergenic | 50.0% | |
| TGTCCATTTGGCCATACCCT+TGG | + | chr1.3:17458712-17458731 | None:intergenic | 50.0% | |
| ! | TAAGCCTTTGGTGTGGTGCA+AGG | - | chr1.3:17458844-17458863 | MS.gene25171:CDS | 50.0% |
| ! | TTCCCAAGGGTATGGCCAAA+TGG | - | chr1.3:17458706-17458725 | MS.gene25171:CDS | 50.0% |
| !! | AGGGGAAGTACCATAGCTCA+AGG | + | chr1.3:17459285-17459304 | None:intergenic | 50.0% |
| !! | CAAGAAGCATTCTTCCAGCC+AGG | - | chr1.3:17459312-17459331 | MS.gene25171:CDS | 50.0% |
| GACTTTATCACTGCCCTGGC+TGG | + | chr1.3:17459329-17459348 | None:intergenic | 55.0% | |
| GGACAATGATGCCATGGCCT+AGG | - | chr1.3:17458727-17458746 | MS.gene25171:CDS | 55.0% | |
| GGTATAGGTAGCCTAGGCCA+TGG | + | chr1.3:17458741-17458760 | None:intergenic | 55.0% | |
| GCCTCCTTGCACCACACCAA+AGG | + | chr1.3:17458851-17458870 | None:intergenic | 60.0% | |
| ! | GCCTTTGGTGTGGTGCAAGG+AGG | - | chr1.3:17458847-17458866 | MS.gene25171:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 17458690 | 17459448 | 17458690 | ID=MS.gene25171 |
| chr1.3 | mRNA | 17458690 | 17459448 | 17458690 | ID=MS.gene25171.t1;Parent=MS.gene25171 |
| chr1.3 | exon | 17459110 | 17459448 | 17459110 | ID=MS.gene25171.t1.exon1;Parent=MS.gene25171.t1 |
| chr1.3 | CDS | 17459110 | 17459448 | 17459110 | ID=cds.MS.gene25171.t1;Parent=MS.gene25171.t1 |
| chr1.3 | exon | 17458690 | 17458869 | 17458690 | ID=MS.gene25171.t1.exon2;Parent=MS.gene25171.t1 |
| chr1.3 | CDS | 17458690 | 17458869 | 17458690 | ID=cds.MS.gene25171.t1;Parent=MS.gene25171.t1 |
| Gene Sequence |
| Protein sequence |
