AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36048


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36048.t1 MTR_8g061350 95.954 173 7 0 1 173 1 173 4.86e-110 311
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36048.t1 AT3G15190 77.876 113 23 1 57 167 75 187 1.26e-56 177

Find 41 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTACTGCCTTCTTTCTTC+TGG 0.196704 8.1:-52588915 None:intergenic
ATAGCGGAATCAACTTTCTT+TGG 0.240354 8.1:-52587660 None:intergenic
CCTAACTTCATCATCAAATT+CGG 0.266189 8.1:+52587527 MS.gene36048:CDS
CCGAATTTGATGATGAAGTT+AGG 0.293575 8.1:-52587527 None:intergenic
CTCTAGACTGGGAAATGTTT+CGG 0.313050 8.1:-52587566 None:intergenic
TCTAGACTGGGAAATGTTTC+GGG 0.313830 8.1:-52587565 None:intergenic
TTACAGTTACGGTACCTTCT+TGG 0.353552 8.1:-52587754 None:intergenic
CTTGGTACGGGTTCTAATCT+CGG 0.388971 8.1:-52587736 None:intergenic
GCCTCAATTGAGAAGATGAT+TGG 0.400874 8.1:+52588809 MS.gene36048:CDS
CTAACTTCATCATCAAATTC+GGG 0.410171 8.1:+52587528 MS.gene36048:CDS
CGAATTTGATGATGAAGTTA+GGG 0.438285 8.1:-52587526 None:intergenic
CAGACACTGGCTTGAGGCAC+AGG 0.442721 8.1:-52588959 None:intergenic
AGGTATGTTATCTTTGAATA+CGG 0.445037 8.1:+52587599 MS.gene36048:CDS
TGAACCTTCCTTCGAAAATG+AGG 0.476736 8.1:+52587496 MS.gene36048:CDS
CTTGTGAAAGGACTCTAGAC+TGG 0.483441 8.1:-52587578 None:intergenic
GTTACGGTACCTTCTTGGTA+CGG 0.485051 8.1:-52587749 None:intergenic
CAGGGGCATACCAGCCATGA+TGG 0.502689 8.1:-52588940 None:intergenic
TCCAATCATCTTCTCAATTG+AGG 0.507360 8.1:-52588810 None:intergenic
TTACGGTACCTTCTTGGTAC+GGG 0.515322 8.1:-52587748 None:intergenic
TTGATGATGAAGTTAGGGTA+AGG 0.521257 8.1:-52587521 None:intergenic
AATCGACAAAGCAGTGAAAG+TGG 0.542695 8.1:+52588847 MS.gene36048:CDS
CAGTAGAGATCCATCATGGC+TGG 0.548246 8.1:+52588930 MS.gene36048:CDS
TCGGCTTGCCAGAAGAAAGA+AGG 0.559181 8.1:+52588907 MS.gene36048:CDS
AAGATAACATACCTTGTGAA+AGG 0.576623 8.1:-52587590 None:intergenic
CGGGAGCAAATCGCAAGTCT+CGG 0.578912 8.1:+52588888 MS.gene36048:CDS
AGACACTGGCTTGAGGCACA+GGG 0.582207 8.1:-52588958 None:intergenic
CAGTCTAGAGTCCTTTCACA+AGG 0.588356 8.1:+52587579 MS.gene36048:CDS
ATATAAAAGGATTCAGACAC+TGG 0.591833 8.1:-52588972 None:intergenic
GATTAGAACCCGTACCAAGA+AGG 0.607531 8.1:+52587740 MS.gene36048:CDS
ATCGACAAAGCAGTGAAAGT+GGG 0.608479 8.1:+52588848 MS.gene36048:CDS
AGAGAGCTCGTCAAGCTGAG+AGG 0.642101 8.1:+52587685 MS.gene36048:CDS
TTGTGAAAGGACTCTAGACT+GGG 0.643097 8.1:-52587577 None:intergenic
TTGAATACGGTGCAGAAGCG+TGG 0.643767 8.1:+52587612 MS.gene36048:CDS
GGGAACTTTGCATAGAAACA+CGG 0.644538 8.1:+52588868 MS.gene36048:CDS
AAGGCAGTAGAGATCCATCA+TGG 0.646488 8.1:+52588926 MS.gene36048:CDS
TGGTGTTGTTGTTGTTTGCG+AGG 0.647337 8.1:+52587632 MS.gene36048:CDS
AGGATTCAGACACTGGCTTG+AGG 0.656971 8.1:-52588965 None:intergenic
GGAACTTTGCATAGAAACAC+GGG 0.657374 8.1:+52588869 MS.gene36048:CDS
TTGACGAGCTCTCTTAATAG+CGG 0.659609 8.1:-52587676 None:intergenic
AATTGAGAAGATGATTGGAG+AGG 0.673219 8.1:+52588814 MS.gene36048:CDS
GACACTGGCTTGAGGCACAG+GGG 0.714773 8.1:-52588957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTCAAATTATTAT+TGG + chr8.1:52587818-52587837 MS.gene36048:intron 15.0%
!! AGAAATAGATGATAATATGA+TGG + chr8.1:52588248-52588267 MS.gene36048:intron 20.0%
!! ATAGAATAGCTATAATAACA+TGG + chr8.1:52587907-52587926 MS.gene36048:intron 20.0%
!! TGATAATATATGCAAGAAAA+GGG + chr8.1:52588217-52588236 MS.gene36048:intron 20.0%
!! TGTAAATAAAACTACAAGTT+GGG - chr8.1:52588692-52588711 None:intergenic 20.0%
!! TTATATGAGTTACACATTAT+GGG - chr8.1:52588173-52588192 None:intergenic 20.0%
!! TTTCTTGCATATATTATCAA+TGG - chr8.1:52588216-52588235 None:intergenic 20.0%
!!! GAAAACATGATTTGTATTTT+AGG + chr8.1:52588083-52588102 MS.gene36048:intron 20.0%
!!! TTGATAATATATGCAAGAAA+AGG + chr8.1:52588216-52588235 MS.gene36048:intron 20.0%
! AGGTATGTTATCTTTGAATA+CGG + chr8.1:52587599-52587618 MS.gene36048:CDS 25.0%
! ATAACTTTCCTTGAGAAATT+TGG + chr8.1:52588189-52588208 MS.gene36048:intron 25.0%
! CTGTAAATAAAACTACAAGT+TGG - chr8.1:52588693-52588712 None:intergenic 25.0%
! GTAAATAAAACTACAAGTTG+GGG - chr8.1:52588691-52588710 None:intergenic 25.0%
! GTTATATGAGTTACACATTA+TGG - chr8.1:52588174-52588193 None:intergenic 25.0%
! TAATTATATAACTTGAGCGA+AGG - chr8.1:52588033-52588052 None:intergenic 25.0%
! TCTCAAATTATTATTGGTGT+CGG + chr8.1:52587824-52587843 MS.gene36048:intron 25.0%
! TCTTACTTTCAATTGTTTGT+AGG + chr8.1:52588730-52588749 MS.gene36048:intron 25.0%
!! AAAATTGATTCCATAGCTTT+AGG + chr8.1:52587976-52587995 MS.gene36048:intron 25.0%
!! AAATTGATTCCATAGCTTTA+GGG + chr8.1:52587977-52587996 MS.gene36048:intron 25.0%
!! AATAGTTCAAGTTACAGTTA+CGG - chr8.1:52587768-52587787 None:intergenic 25.0%
!! ATGATAATATGATGGAGTAT+GGG + chr8.1:52588256-52588275 MS.gene36048:intron 25.0%
!! TTTCAATTGTTTGTAGGTTT+TGG + chr8.1:52588736-52588755 MS.gene36048:intron 25.0%
!!! CAACTTGTAGTTTTATTTAC+AGG + chr8.1:52588691-52588710 MS.gene36048:intron 25.0%
!!! CATGTTTTCAAGATGAAATT+GGG - chr8.1:52588072-52588091 None:intergenic 25.0%
!!! TCATGTTTTCAAGATGAAAT+TGG - chr8.1:52588073-52588092 None:intergenic 25.0%
AAGATAACATACCTTGTGAA+AGG - chr8.1:52587593-52587612 None:intergenic 30.0%
ATAGCTATTCTATGAAGAAC+TGG - chr8.1:52587900-52587919 None:intergenic 30.0%
CAGAAACTAGAACTAAAACA+TGG - chr8.1:52588421-52588440 None:intergenic 30.0%
CCTAACTTCATCATCAAATT+CGG + chr8.1:52587527-52587546 MS.gene36048:CDS 30.0%
CTAACTTCATCATCAAATTC+GGG + chr8.1:52587528-52587547 MS.gene36048:CDS 30.0%
TGACAATTCAACAGTTTAGA+AGG + chr8.1:52588387-52588406 MS.gene36048:intron 30.0%
! AAGCTATGGAATCAATTTTC+AGG - chr8.1:52587975-52587994 None:intergenic 30.0%
! ATCATCAGAAAATGTTGCTT+CGG - chr8.1:52588312-52588331 None:intergenic 30.0%
! GATGATAATATGATGGAGTA+TGG + chr8.1:52588255-52588274 MS.gene36048:intron 30.0%
!! CGAATTTGATGATGAAGTTA+GGG - chr8.1:52587529-52587548 None:intergenic 30.0%
AAAAACAAGAACACAACACC+TGG - chr8.1:52588117-52588136 None:intergenic 35.0%
AAAACAAGAACACAACACCT+GGG - chr8.1:52588116-52588135 None:intergenic 35.0%
AACAATAAGAATCCCTGACA+TGG + chr8.1:52588578-52588597 MS.gene36048:intron 35.0%
AATTGAGAAGATGATTGGAG+AGG + chr8.1:52588814-52588833 MS.gene36048:CDS 35.0%
ATGAAATTGGGTGATGACAA+TGG - chr8.1:52588060-52588079 None:intergenic 35.0%
GTAGCTGATGTTATAATTGC+AGG + chr8.1:52588600-52588619 MS.gene36048:intron 35.0%
GTGGAGTTTCATTATCTTAC+AGG - chr8.1:52588557-52588576 None:intergenic 35.0%
TCAATGGTCCAAATTTCTCA+AGG - chr8.1:52588200-52588219 None:intergenic 35.0%
TCAGGGATTCTTATTGTTTG+TGG - chr8.1:52588576-52588595 None:intergenic 35.0%
TCCAATCATCTTCTCAATTG+AGG - chr8.1:52588813-52588832 None:intergenic 35.0%
! ATAAAACTACAAGTTGGGGT+TGG - chr8.1:52588687-52588706 None:intergenic 35.0%
! ATAGCGGAATCAACTTTCTT+TGG - chr8.1:52587663-52587682 None:intergenic 35.0%
!! CCGAATTTGATGATGAAGTT+AGG - chr8.1:52587530-52587549 None:intergenic 35.0%
!! TTGATGATGAAGTTAGGGTA+AGG - chr8.1:52587524-52587543 None:intergenic 35.0%
AATCGACAAAGCAGTGAAAG+TGG + chr8.1:52588847-52588866 MS.gene36048:CDS 40.0%
AGAGGGAAGTTTGAGTAACA+TGG + chr8.1:52588003-52588022 MS.gene36048:intron 40.0%
ATAACATCAGCTACCATGTC+AGG - chr8.1:52588594-52588613 None:intergenic 40.0%
ATCGACAAAGCAGTGAAAGT+GGG + chr8.1:52588848-52588867 MS.gene36048:CDS 40.0%
CTCTAGACTGGGAAATGTTT+CGG - chr8.1:52587569-52587588 None:intergenic 40.0%
GCCTCAATTGAGAAGATGAT+TGG + chr8.1:52588809-52588828 MS.gene36048:CDS 40.0%
GGAACTTTGCATAGAAACAC+GGG + chr8.1:52588869-52588888 MS.gene36048:CDS 40.0%
GGGAACTTTGCATAGAAACA+CGG + chr8.1:52588868-52588887 MS.gene36048:CDS 40.0%
TAACATCAGCTACCATGTCA+GGG - chr8.1:52588593-52588612 None:intergenic 40.0%
TCTAGACTGGGAAATGTTTC+GGG - chr8.1:52587568-52587587 None:intergenic 40.0%
TGAACCTTCCTTCGAAAATG+AGG + chr8.1:52587496-52587515 MS.gene36048:CDS 40.0%
TTACAGTTACGGTACCTTCT+TGG - chr8.1:52587757-52587776 None:intergenic 40.0%
TTGACGAGCTCTCTTAATAG+CGG - chr8.1:52587679-52587698 None:intergenic 40.0%
TTGTGAAAGGACTCTAGACT+GGG - chr8.1:52587580-52587599 None:intergenic 40.0%
! ATGATGGAGTATGGGATAGT+GGG + chr8.1:52588264-52588283 MS.gene36048:intron 40.0%
! GTAAGGTTCCTCATTTTCGA+AGG - chr8.1:52587507-52587526 None:intergenic 40.0%
! TATGATGGAGTATGGGATAG+TGG + chr8.1:52588263-52588282 MS.gene36048:intron 40.0%
!! CCATAGCTTTAGGGATTAGA+GGG + chr8.1:52587986-52588005 MS.gene36048:intron 40.0%
!! TATTATTGGTGTCGGCATGT+TGG + chr8.1:52587832-52587851 MS.gene36048:intron 40.0%
!! TCCATAGCTTTAGGGATTAG+AGG + chr8.1:52587985-52588004 MS.gene36048:intron 40.0%
!! TGTTTCAAGTTACTGAACCC+TGG + chr8.1:52588332-52588351 MS.gene36048:intron 40.0%
!!! TTTGTAGGTTTTGGAAGCAC+TGG + chr8.1:52588745-52588764 MS.gene36048:intron 40.0%
AAGGCAGTAGAGATCCATCA+TGG + chr8.1:52588926-52588945 MS.gene36048:CDS 45.0%
CCCTCTAATCCCTAAAGCTA+TGG - chr8.1:52587989-52588008 None:intergenic 45.0%
CTCTACTGCCTTCTTTCTTC+TGG - chr8.1:52588918-52588937 None:intergenic 45.0%
CTTGTGAAAGGACTCTAGAC+TGG - chr8.1:52587581-52587600 None:intergenic 45.0%
GATTAGAACCCGTACCAAGA+AGG + chr8.1:52587740-52587759 MS.gene36048:CDS 45.0%
GTTACGGTACCTTCTTGGTA+CGG - chr8.1:52587752-52587771 None:intergenic 45.0%
TTACGGTACCTTCTTGGTAC+GGG - chr8.1:52587751-52587770 None:intergenic 45.0%
! CAGTCTAGAGTCCTTTCACA+AGG + chr8.1:52587579-52587598 MS.gene36048:CDS 45.0%
! CTTGGTACGGGTTCTAATCT+CGG - chr8.1:52587739-52587758 None:intergenic 45.0%
! GGTTCCTCATTTTCGAAGGA+AGG - chr8.1:52587503-52587522 None:intergenic 45.0%
! TTCAAGTTACTGAACCCTGG+AGG + chr8.1:52588335-52588354 MS.gene36048:intron 45.0%
!! GTATTTTAGGTGTCAGACCC+AGG + chr8.1:52588096-52588115 MS.gene36048:intron 45.0%
!! TGGGATAGTGGGAGAGAAAA+TGG + chr8.1:52588275-52588294 MS.gene36048:intron 45.0%
!! TGGTGTTGTTGTTGTTTGCG+AGG + chr8.1:52587632-52587651 MS.gene36048:CDS 45.0%
ACTGGCACAAACACAGACAC+CGG - chr8.1:52587882-52587901 None:intergenic 50.0%
AGGCGCAAATTAGGTGGTAC+TGG + chr8.1:52588355-52588374 MS.gene36048:intron 50.0%
CAGTAGAGATCCATCATGGC+TGG + chr8.1:52588930-52588949 MS.gene36048:CDS 50.0%
TTGAATACGGTGCAGAAGCG+TGG + chr8.1:52587612-52587631 MS.gene36048:CDS 50.0%
! TCGGCTTGCCAGAAGAAAGA+AGG + chr8.1:52588907-52588926 MS.gene36048:CDS 50.0%
ACAGACACCGGACACAACAC+TGG - chr8.1:52587870-52587889 None:intergenic 55.0%
AGAGAGCTCGTCAAGCTGAG+AGG + chr8.1:52587685-52587704 MS.gene36048:CDS 55.0%
CGGGAGCAAATCGCAAGTCT+CGG + chr8.1:52588888-52588907 MS.gene36048:CDS 55.0%
! ACCACCTAATTTGCGCCTCC+AGG - chr8.1:52588353-52588372 None:intergenic 55.0%
! CCACCTAATTTGCGCCTCCA+GGG - chr8.1:52588352-52588371 None:intergenic 55.0%
! GAACCCTGGAGGCGCAAATT+AGG + chr8.1:52588346-52588365 MS.gene36048:intron 55.0%
!! AGACACTGGCTTGAGGCACA+GGG - chr8.1:52588961-52588980 None:intergenic 55.0%
!! GTCGGCATGTTGGTGTTGGT+TGG + chr8.1:52587842-52587861 MS.gene36048:intron 55.0%
!! GTTGGTGCCAGTGTTGTGTC+CGG + chr8.1:52587860-52587879 MS.gene36048:intron 55.0%
!! TGGTGTCGGCATGTTGGTGT+TGG + chr8.1:52587838-52587857 MS.gene36048:intron 55.0%
! CAGGGGCATACCAGCCATGA+TGG - chr8.1:52588943-52588962 None:intergenic 60.0%
! CCCTGGAGGCGCAAATTAGG+TGG + chr8.1:52588349-52588368 MS.gene36048:intron 60.0%
!! CAGACACTGGCTTGAGGCAC+AGG - chr8.1:52588962-52588981 None:intergenic 60.0%
!! GACACTGGCTTGAGGCACAG+GGG - chr8.1:52588960-52588979 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 52587471 52588982 52587471 ID=MS.gene36048
chr8.1 mRNA 52587471 52588982 52587471 ID=MS.gene36048.t1;Parent=MS.gene36048
chr8.1 exon 52587471 52587761 52587471 ID=MS.gene36048.t1.exon1;Parent=MS.gene36048.t1
chr8.1 CDS 52587471 52587761 52587471 ID=cds.MS.gene36048.t1;Parent=MS.gene36048.t1
chr8.1 exon 52588752 52588982 52588752 ID=MS.gene36048.t1.exon2;Parent=MS.gene36048.t1
chr8.1 CDS 52588752 52588982 52588752 ID=cds.MS.gene36048.t1;Parent=MS.gene36048.t1
Gene Sequence

>MS.gene36048

ATGGCTTCAGTTTCATGTTCATTGTTGAACCTTCCTTCGAAAATGAGGAACCTTACCCTAACTTCATCATCAAATTCGGGTTCTCTAAGTTTCTCCCGAAACATTTCCCAGTCTAGAGTCCTTTCACAAGGTATGTTATCTTTGAATACGGTGCAGAAGCGTGGTGTTGTTGTTGTTTGCGAGGCTGCTCCAAAGAAAGTTGATTCCGCTATTAAGAGAGCTCGTCAAGCTGAGAGGCGTCGCGTTTACAACAAAGCTCGTAAATCCGAGATTAGAACCCGTACCAAGAAGGTACCGTAACTGTAACTTGAACTATTTTATTTTTTTATTACTATATCATAGCTATGTCATTTTCTCAAATTATTATTGGTGTCGGCATGTTGGTGTTGGTTGGTGCCAGTGTTGTGTCCGGTGTCTGTGTTTGTGCCAGTTCTTCATAGAATAGCTATAATAACATGGTTTTAACTTTTAAGCGTCTTTTAATATGAAATGTTATGTGTGCCTGAAAATTGATTCCATAGCTTTAGGGATTAGAGGGAAGTTTGAGTAACATGGTTTTCCTTCGCTCAAGTTATATAATTATTCGCCATTGTCATCACCCAATTTCATCTTGAAAACATGATTTGTATTTTAGGTGTCAGACCCAGGTGTTGTGTTCTTGTTTTTGTTATAGAGTGATGTTAATCATAATCATAATCACCCATAATGTGTAACTCATATAACTTTCCTTGAGAAATTTGGACCATTGATAATATATGCAAGAAAAGGGAGTGAAAGAGAAATAGATGATAATATGATGGAGTATGGGATAGTGGGAGAGAAAATGGAAAATGTTATGCCGAAGCAACATTTTCTGATGATTGTTTCAAGTTACTGAACCCTGGAGGCGCAAATTAGGTGGTACTGGAAACATGTTTGACAATTCAACAGTTTAGAAGGAGAAGTGTCCATGTTTTAGTTCTAGTTTCTGACGATTATATACGATAATAATATTTTAGTGTGACTGCTGATGTAATATACTATGATAGTGCTTTTCTAAGTCAAATTGTTATACAATTAATATTGAATTTAAAAATGAGTTAACCTGTAAGATAATGAAACTCCACAAACAATAAGAATCCCTGACATGGTAGCTGATGTTATAATTGCAGGTGCTTATACTGATTATTCTTGTCAAAACTCAAGTTAACATGCGTTTTTATTATCTATACAACCAACCCCAACTTGTAGTTTTATTTACAGGAATGATTTTTTAGTTATCTTACTTTCAATTGTTTGTAGGTTTTGGAAGCACTGGAGTTGCTTAAAAAGAAATCTGATGCACAAGCAGAAGAAATTGCCTCAATTGAGAAGATGATTGGAGAGGCATACTCTATAATCGACAAAGCAGTGAAAGTGGGAACTTTGCATAGAAACACGGGAGCAAATCGCAAGTCTCGGCTTGCCAGAAGAAAGAAGGCAGTAGAGATCCATCATGGCTGGTATGCCCCTGTGCCTCAAGCCAGTGTCTGA

Protein sequence

>MS.gene36048.t1

MASVSCSLLNLPSKMRNLTLTSSSNSGSLSFSRNISQSRVLSQGMLSLNTVQKRGVVVVCEAAPKKVDSAIKRARQAERRRVYNKARKSEIRTRTKKVLEALELLKKKSDAQAEEIASIEKMIGEAYSIIDKAVKVGTLHRNTGANRKSRLARRKKAVEIHHGWYAPVPQASV