AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062252


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062252.t1 MTR_1g076310 98.214 112 2 0 1 112 1 112 1.06e-79 230
MS.gene062252.t1 MTR_2g039750 59.048 105 36 1 1 98 1 105 2.36e-39 128
MS.gene062252.t1 MTR_5g014070 58.095 105 37 1 1 98 1 105 3.80e-38 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062252.t1 AT4G34620 65.854 82 28 0 1 82 1 82 4.62e-38 124
MS.gene062252.t1 AT5G56940 53.846 104 41 1 1 97 1 104 5.68e-38 125

Find 23 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACAACAACCCGATAAAA+TGG 0.179817 1.1:+57172254 None:intergenic
TGGATGGCTGCTCTTCGTTC+GGG 0.277399 1.1:+57170520 None:intergenic
TTGGATGGCTGCTCTTCGTT+CGG 0.292007 1.1:+57170519 None:intergenic
ACCATCAACTTCTTTGTCAT+TGG 0.341893 1.1:+57170500 None:intergenic
GGTTACTACAATCCTATTGC+AGG 0.364917 1.1:-57172188 MS.gene062252:intron
TCTTCGTTCGGGGTATCAAC+AGG 0.369294 1.1:+57170531 None:intergenic
ATTGATATTCAACCTGCAAT+AGG 0.373630 1.1:+57172176 None:intergenic
GGCAAGAACATTGAAGTTGT+TGG 0.440064 1.1:-57172209 MS.gene062252:CDS
TCCAATGACAAAGAAGTTGA+TGG 0.440759 1.1:-57170501 MS.gene062252:CDS
TTTAGGTATTGGCTTTCTGT+TGG 0.451329 1.1:-57170639 MS.gene062252:intron
AAACGGAAAGGTGGACTGTC+TGG 0.470726 1.1:-57170561 MS.gene062252:CDS
TCAACTTCTTTGTCATTGGA+TGG 0.482393 1.1:+57170504 None:intergenic
AGGCAGGTTTACAAATGAAA+CGG 0.502953 1.1:-57170578 MS.gene062252:CDS
AGTATTCGATTAGCGAGACT+TGG 0.516156 1.1:-57172290 MS.gene062252:CDS
TTACAACAACCCGATAAAAT+GGG 0.516278 1.1:+57172255 None:intergenic
AGACTCTCCACAGGTTCTGA+AGG 0.549004 1.1:+57170609 None:intergenic
GATAGCAAATGTGCAAGAGA+TGG 0.580660 1.1:-57172230 MS.gene062252:CDS
TGCTCAACCTTCAGAACCTG+TGG 0.580737 1.1:-57170616 MS.gene062252:CDS
TTGAATAAAAGACTCTCCAC+AGG 0.595461 1.1:+57170600 None:intergenic
GGATGGCTGCTCTTCGTTCG+GGG 0.597237 1.1:+57170521 None:intergenic
CTTCGTTCGGGGTATCAACA+GGG 0.597733 1.1:+57170532 None:intergenic
TTACAAATGAAACGGAAAGG+TGG 0.647965 1.1:-57170570 MS.gene062252:CDS
GGTTTACAAATGAAACGGAA+AGG 0.664042 1.1:-57170573 MS.gene062252:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTAAAAAAAATTAAA+TGG + chr1.1:57171427-57171446 None:intergenic 0.0%
!!! TTTAATTTTTTTTAAAATTA+CGG - chr1.1:57171427-57171446 MS.gene062252:intron 0.0%
!! AAAATTTCAGAAAATTATTA+TGG + chr1.1:57171334-57171353 None:intergenic 10.0%
!! TTTATTGATATTGAATATAT+AGG - chr1.1:57171003-57171022 MS.gene062252:intron 10.0%
!!! TATATGCAATATTTTATTTT+TGG + chr1.1:57171173-57171192 None:intergenic 10.0%
!! AAAGTAAATCAAGTTAAAAA+AGG + chr1.1:57171953-57171972 None:intergenic 15.0%
!!! AAAGAATTTTTCTTTCATTT+GGG - chr1.1:57170735-57170754 MS.gene062252:intron 15.0%
!! AATCAAGTTAAAAAAGGATT+AGG + chr1.1:57171947-57171966 None:intergenic 20.0%
!! ATGATTTACAATTACATACA+TGG - chr1.1:57171461-57171480 MS.gene062252:intron 20.0%
!! CAAATGAAAGAAAAATTCTT+TGG + chr1.1:57170736-57170755 None:intergenic 20.0%
!! TATGAATATTGCATATAGAA+GGG - chr1.1:57171141-57171160 MS.gene062252:intron 20.0%
!! TGTTTATAAATGATAAAACC+AGG + chr1.1:57171754-57171773 None:intergenic 20.0%
!! TTATGAATATTGCATATAGA+AGG - chr1.1:57171140-57171159 MS.gene062252:intron 20.0%
!!! AAGAATTTTTCTTTCATTTG+GGG - chr1.1:57170736-57170755 MS.gene062252:intron 20.0%
!!! AGTTATTTGTACACAATTTT+GGG - chr1.1:57172058-57172077 MS.gene062252:intron 20.0%
!!! CAAAGAATTTTTCTTTCATT+TGG - chr1.1:57170734-57170753 MS.gene062252:intron 20.0%
!!! CATTTTTAATCCTTTTACTA+TGG + chr1.1:57171834-57171853 None:intergenic 20.0%
!!! GGTTTTATCATTTATAAACA+CGG - chr1.1:57171754-57171773 MS.gene062252:intron 20.0%
!!! GTTCAAATAACATTACTTTT+CGG - chr1.1:57170936-57170955 MS.gene062252:intron 20.0%
!!! TAGTTATTTGTACACAATTT+TGG - chr1.1:57172057-57172076 MS.gene062252:intron 20.0%
!!! TTAAAGCACAAAATTGTTTT+GGG - chr1.1:57170782-57170801 MS.gene062252:CDS 20.0%
!!! TTCAAATAACATTACTTTTC+GGG - chr1.1:57170937-57170956 MS.gene062252:intron 20.0%
! AACACATAGAATTACAGATA+AGG + chr1.1:57170632-57170651 None:intergenic 25.0%
! AACGAACTGATGTATTTAAA+CGG - chr1.1:57171618-57171637 MS.gene062252:intron 25.0%
! AGCATACAATAATGTTCTAA+AGG + chr1.1:57171286-57171305 None:intergenic 25.0%
! GAAAATTATTATGGCAAGTA+AGG + chr1.1:57171325-57171344 None:intergenic 25.0%
! GAGATAAAGATAAACGTATA+TGG - chr1.1:57170896-57170915 MS.gene062252:intron 25.0%
! TTACAATTACATACATGGTA+TGG - chr1.1:57171466-57171485 MS.gene062252:intron 25.0%
! TTATCATTTATAAACACGGA+GGG - chr1.1:57171758-57171777 MS.gene062252:intron 25.0%
! TTGTTAATATCTCTTGTTAG+TGG - chr1.1:57171256-57171275 MS.gene062252:intron 25.0%
! TTTATCATTTATAAACACGG+AGG - chr1.1:57171757-57171776 MS.gene062252:intron 25.0%
!! ATATATAGGACTTGTTTGAA+AGG - chr1.1:57171017-57171036 MS.gene062252:intron 25.0%
!! ATCCTTTTACTATGGAATTA+TGG + chr1.1:57171826-57171845 None:intergenic 25.0%
!! CTTAAAGCACAAAATTGTTT+TGG - chr1.1:57170781-57170800 MS.gene062252:CDS 25.0%
!! GTTATTTGTACACAATTTTG+GGG - chr1.1:57172059-57172078 MS.gene062252:intron 25.0%
!! TTCTACTTTGTAAAGTATCA+TGG - chr1.1:57171671-57171690 MS.gene062252:intron 25.0%
AAATGACCATAATGAAACAC+AGG - chr1.1:57171217-57171236 MS.gene062252:intron 30.0%
AATTGCAATGTATACCAGTT+TGG - chr1.1:57171888-57171907 MS.gene062252:intron 30.0%
ATTAAATGGCAGATTGACAT+AGG + chr1.1:57171413-57171432 None:intergenic 30.0%
ATTGATATTCAACCTGCAAT+AGG + chr1.1:57170602-57170621 None:intergenic 30.0%
ATTGCAATGTATACCAGTTT+GGG - chr1.1:57171889-57171908 MS.gene062252:intron 30.0%
CTTATCTGTTTGCATCTTAA+TGG - chr1.1:57171544-57171563 MS.gene062252:intron 30.0%
GCAATTCACAGTAACAAATA+GGG + chr1.1:57171874-57171893 None:intergenic 30.0%
GGAAATTAGTGATAATTGTG+GGG - chr1.1:57172080-57172099 MS.gene062252:intron 30.0%
GGGAAATTAGTGATAATTGT+GGG - chr1.1:57172079-57172098 MS.gene062252:intron 30.0%
TCAACTTGAAATGAAAACAC+CGG + chr1.1:57171375-57171394 None:intergenic 30.0%
TGCAATTCACAGTAACAAAT+AGG + chr1.1:57171875-57171894 None:intergenic 30.0%
TTACAACAACCCGATAAAAT+GGG + chr1.1:57170523-57170542 None:intergenic 30.0%
TTGTATGCTTGATTTCACTT+TGG - chr1.1:57171297-57171316 MS.gene062252:intron 30.0%
! GAATTTTCTGTGTTTACGAT+AGG - chr1.1:57171488-57171507 MS.gene062252:intron 30.0%
! TTCATTTGGGGTATACTTTA+TGG - chr1.1:57170748-57170767 MS.gene062252:intron 30.0%
!!! TTTTTTGTTCCTAGCTCTAA+GGG + chr1.1:57171115-57171134 None:intergenic 30.0%
AAACACCGGAAAAACTTACA+CGG + chr1.1:57171361-57171380 None:intergenic 35.0%
ACCATAATGAAACACAGGTT+TGG - chr1.1:57171222-57171241 MS.gene062252:intron 35.0%
ACCATCAACTTCTTTGTCAT+TGG + chr1.1:57172278-57172297 None:intergenic 35.0%
AGGCAGGTTTACAAATGAAA+CGG - chr1.1:57172197-57172216 MS.gene062252:CDS 35.0%
CAAATATAGTGTACTCCGTT+TGG - chr1.1:57170842-57170861 MS.gene062252:intron 35.0%
CAATACCTAAAGAACATGAG+GGG + chr1.1:57172127-57172146 None:intergenic 35.0%
CCAATACCTAAAGAACATGA+GGG + chr1.1:57172128-57172147 None:intergenic 35.0%
CTGATTTCTGTTCTCAGTAT+GGG - chr1.1:57171520-57171539 MS.gene062252:intron 35.0%
GGCCATAATTCCATAGTAAA+AGG - chr1.1:57171821-57171840 MS.gene062252:intron 35.0%
GGGGAAATTAGTGATAATTG+TGG - chr1.1:57172078-57172097 MS.gene062252:intron 35.0%
GGTTTACAAATGAAACGGAA+AGG - chr1.1:57172202-57172221 MS.gene062252:CDS 35.0%
GTGAATGTCTAAGAGAGTTT+GGG - chr1.1:57171056-57171075 MS.gene062252:intron 35.0%
GTTACAACAACCCGATAAAA+TGG + chr1.1:57170524-57170543 None:intergenic 35.0%
TAATCAATGACCCAAACTGT+TGG - chr1.1:57171800-57171819 MS.gene062252:intron 35.0%
TATCTTCTGCAACATCCAAA+CGG + chr1.1:57170860-57170879 None:intergenic 35.0%
TCAACTTCTTTGTCATTGGA+TGG + chr1.1:57172274-57172293 None:intergenic 35.0%
TCCAAACCTGTGTTTCATTA+TGG + chr1.1:57171226-57171245 None:intergenic 35.0%
TCCAATGACAAAGAAGTTGA+TGG - chr1.1:57172274-57172293 MS.gene062252:CDS 35.0%
TGTGAATGTCTAAGAGAGTT+TGG - chr1.1:57171055-57171074 MS.gene062252:intron 35.0%
TTACAAATGAAACGGAAAGG+TGG - chr1.1:57172205-57172224 MS.gene062252:CDS 35.0%
TTGAATAAAAGACTCTCCAC+AGG + chr1.1:57172178-57172197 None:intergenic 35.0%
! ATTTGGGGTATACTTTATGG+TGG - chr1.1:57170751-57170770 MS.gene062252:intron 35.0%
! TGTGGAGAGTCTTTTATTCA+AGG - chr1.1:57172177-57172196 MS.gene062252:intron 35.0%
! TTGTTCCGTGTAAGTTTTTC+CGG - chr1.1:57171353-57171372 MS.gene062252:intron 35.0%
!! CTTTTTCGCTAATGAGTAGT+GGG - chr1.1:57170980-57170999 MS.gene062252:intron 35.0%
!! TTTAGGTATTGGCTTTCTGT+TGG - chr1.1:57172136-57172155 MS.gene062252:intron 35.0%
!!! GGGAGAAACACGTTTTTTTA+TGG - chr1.1:57170957-57170976 MS.gene062252:intron 35.0%
!!! GTTTTTTGTTCCTAGCTCTA+AGG + chr1.1:57171116-57171135 None:intergenic 35.0%
AGTATTCGATTAGCGAGACT+TGG - chr1.1:57170485-57170504 MS.gene062252:CDS 40.0%
CCATACTGAGAACAGAAATC+AGG + chr1.1:57171522-57171541 None:intergenic 40.0%
CCCTCATGTTCTTTAGGTAT+TGG - chr1.1:57172125-57172144 MS.gene062252:intron 40.0%
CCTGATTTCTGTTCTCAGTA+TGG - chr1.1:57171519-57171538 MS.gene062252:intron 40.0%
CTAGAAAGTGACCTGATAGA+GGG - chr1.1:57171727-57171746 MS.gene062252:intron 40.0%
GATAGCAAATGTGCAAGAGA+TGG - chr1.1:57170545-57170564 MS.gene062252:CDS 40.0%
GCCAATACCTAAAGAACATG+AGG + chr1.1:57172129-57172148 None:intergenic 40.0%
GGAATTATGGCCAACAGTTT+GGG + chr1.1:57171813-57171832 None:intergenic 40.0%
GGCAAGAACATTGAAGTTGT+TGG - chr1.1:57170566-57170585 MS.gene062252:CDS 40.0%
GGCAGATTGACATAGGTTAT+AGG + chr1.1:57171406-57171425 None:intergenic 40.0%
GGTTACTACAATCCTATTGC+AGG - chr1.1:57170587-57170606 MS.gene062252:CDS 40.0%
GTGATAATTGTGGGGATCAT+TGG - chr1.1:57172088-57172107 MS.gene062252:intron 40.0%
TAGAAAGTGACCTGATAGAG+GGG - chr1.1:57171728-57171747 MS.gene062252:intron 40.0%
TGGAATTATGGCCAACAGTT+TGG + chr1.1:57171814-57171833 None:intergenic 40.0%
TTTAGCTATCCCTTAGAGCT+AGG - chr1.1:57171103-57171122 MS.gene062252:intron 40.0%
! CACACACAACCCATTTTATC+GGG - chr1.1:57170511-57170530 MS.gene062252:CDS 40.0%
! GAGAGTCTTTTATTCAAGGC+AGG - chr1.1:57172181-57172200 MS.gene062252:intron 40.0%
! GCACACACAACCCATTTTAT+CGG - chr1.1:57170510-57170529 MS.gene062252:CDS 40.0%
! TATACCAGTTTGGGATGAGA+AGG - chr1.1:57171898-57171917 MS.gene062252:intron 40.0%
!! AAACGGATTGAAGTCACTTG+AGG - chr1.1:57171635-57171654 MS.gene062252:intron 40.0%
!!! GCTTTTTCGCTAATGAGTAG+TGG - chr1.1:57170979-57170998 MS.gene062252:intron 40.0%
AACATGAGGGGAGGTTAAGA+TGG + chr1.1:57172115-57172134 None:intergenic 45.0%
AACCTCCCCTCATGTTCTTT+AGG - chr1.1:57172119-57172138 MS.gene062252:intron 45.0%
GCTAGAAAGTGACCTGATAG+AGG - chr1.1:57171726-57171745 MS.gene062252:intron 45.0%
TACCTAAAGAACATGAGGGG+AGG + chr1.1:57172124-57172143 None:intergenic 45.0%
TAAAACCAGGCCCCTCTATC+AGG + chr1.1:57171741-57171760 None:intergenic 50.0%
TGCTCAACCTTCAGAACCTG+TGG - chr1.1:57172159-57172178 MS.gene062252:intron 50.0%
TTGGATGGCTGCTCTTCGTT+CGG + chr1.1:57172259-57172278 None:intergenic 50.0%
! ACCAGTTTGGGATGAGAAGG+AGG - chr1.1:57171901-57171920 MS.gene062252:intron 50.0%
! AGACTCTCCACAGGTTCTGA+AGG + chr1.1:57172169-57172188 None:intergenic 50.0%
! CCAGTTTGGGATGAGAAGGA+GGG - chr1.1:57171902-57171921 MS.gene062252:intron 50.0%
! CTTCGTTCGGGGTATCAACA+GGG + chr1.1:57172246-57172265 None:intergenic 50.0%
! TCTTCGTTCGGGGTATCAAC+AGG + chr1.1:57172247-57172266 None:intergenic 50.0%
!! AAACGGAAAGGTGGACTGTC+TGG - chr1.1:57172214-57172233 MS.gene062252:CDS 50.0%
CCCTCCTTCTCATCCCAAAC+TGG + chr1.1:57171905-57171924 None:intergenic 55.0%
TGGATGGCTGCTCTTCGTTC+GGG + chr1.1:57172258-57172277 None:intergenic 55.0%
AGTGACCTGATAGAGGGGCC+TGG - chr1.1:57171733-57171752 MS.gene062252:intron 60.0%
GGATGGCTGCTCTTCGTTCG+GGG + chr1.1:57172257-57172276 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 57170476 57172321 57170476 ID=MS.gene062252
chr1.1 mRNA 57170476 57172321 57170476 ID=MS.gene062252.t1;Parent=MS.gene062252
chr1.1 exon 57172189 57172321 57172189 ID=MS.gene062252.t1.exon1;Parent=MS.gene062252.t1
chr1.1 CDS 57172189 57172321 57172189 ID=cds.MS.gene062252.t1;Parent=MS.gene062252.t1
chr1.1 exon 57170772 57170817 57170772 ID=MS.gene062252.t1.exon2;Parent=MS.gene062252.t1
chr1.1 CDS 57170772 57170817 57170772 ID=cds.MS.gene062252.t1;Parent=MS.gene062252.t1
chr1.1 exon 57170476 57170656 57170476 ID=MS.gene062252.t1.exon3;Parent=MS.gene062252.t1
chr1.1 CDS 57170476 57170656 57170476 ID=cds.MS.gene062252.t1;Parent=MS.gene062252.t1
Gene Sequence

>MS.gene062252

ATGGTGGTGAGTATTCGATTAGCGAGACTTGGATGCACACACAACCCATTTTATCGGGTTGTTGTAACTGATAGCAAATGTGCAAGAGATGGCAAGAACATTGAAGTTGTTGGTTACTACAATCCTATTGCAGGTTGAATATCAATTCTCTAACCTTATCTGTAATTCTATGTGTTTTTATGCTCAAATTTTACTTGTTCAAAAGAAATTTTGTTAGTTATGTTATTAAAAATTGCATTTTTATTACTTTGTGAAAGCCAAAGAATTTTTCTTTCATTTGGGGTATACTTTATGGTGGATTAGTACTTAAAGCACAAAATTGTTTTGGGATAGAGAAATGATATTTTTACATTAAAATTTTGATGTCAAATATAGTGTACTCCGTTTGGATGTTGCAGAAGATAAAACATAAGAGAGAAAGAGATAAAGATAAACGTATATGGTGTGTCTGTATTTTGATGTTCAAATAACATTACTTTTCGGGAGAAACACGTTTTTTTATGGCTTTTTCGCTAATGAGTAGTGGGTTTATTGATATTGAATATATAGGACTTGTTTGAAAGGATATAGAATTAGTGTTGTGAATGTCTAAGAGAGTTTGGGATGTAATGAAATAGTAAGAATCACTTTAGCTATCCCTTAGAGCTAGGAACAAAAAACACAGTTATGAATATTGCATATAGAAGGGTCAACACCAAAAATAAAATATTGCATATAGAAACTATCTTGAATTTTTCGCTCAAATGACCATAATGAAACACAGGTTTGGATCATCGATCGTTGTTAATATCTCTTGTTAGTGGCGTTCCTTTAGAACATTATTGTATGCTTGATTTCACTTTGGTTCCTTACTTGCCATAATAATTTTCTGAAATTTTTGTTCCGTGTAAGTTTTTCCGGTGTTTTCATTTCAAGTTGAAACATGTACCTATAACCTATGTCAATCTGCCATTTAATTTTTTTTAAAATTACGGTGATGTGACTAATGATTTACAATTACATACATGGTATGGAATTTTCTGTGTTTACGATAGGTTCATTAACCTGATTTCTGTTCTCAGTATGGGACTTATCTGTTTGCATCTTAATGGACTTAGTAGTGACTTTTATCTATATATTAAGCGAGTGACATATTTAATTATAACGAACTGATGTATTTAAACGGATTGAAGTCACTTGAGGTTTCATTCAGATGTTCTACTTTGTAAAGTATCATGGCGCATAAATTGTTATCTGCATCACTATTTTTTGCTAGAAAGTGACCTGATAGAGGGGCCTGGTTTTATCATTTATAAACACGGAGGGATTTGATTGTTTCAAAAATTAATCAATGACCCAAACTGTTGGCCATAATTCCATAGTAAAAGGATTAAAAATGCAAGTTGCAAATGTCATCCCTATTTGTTACTGTGAATTGCAATGTATACCAGTTTGGGATGAGAAGGAGGGTGATTCAAGTTGATACACACCTAATCCTTTTTTAACTTGATTTACTTTTGTACAGCTAAAGATGATGAGAAAAGAGTGAACATCAAACTTGAAAGAGTGAAGTAAGTAACTTTTATTTAATTCTAATTTATCTAGTTATTTGTACACAATTTTGGGGAAATTAGTGATAATTGTGGGGATCATTGGTCCATCTTAACCTCCCCTCATGTTCTTTAGGTATTGGCTTTCTGTTGGTGCTCAACCTTCAGAACCTGTGGAGAGTCTTTTATTCAAGGCAGGTTTACAAATGAAACGGAAAGGTGGACTGTCTGGTAGCTACCCTGTTGATACCCCGAACGAAGAGCAGCCATCCAATGACAAAGAAGTTGATGGTAAACTGACACTCCTTCACTTTTGA

Protein sequence

>MS.gene062252.t1

MVVSIRLARLGCTHNPFYRVVVTDSKCARDGKNIEVVGYYNPIAAKDDEKRVNIKLERVKYWLSVGAQPSEPVESLLFKAGLQMKRKGGLSGSYPVDTPNEEQPSNDKEVDGKLTLLHF