AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26990.t1 MTR_1g022450 98.507 134 2 0 1 134 1 134 1.81e-96 273
MS.gene26990.t1 MTR_1g022450 97.059 136 2 1 1 134 1 136 1.30e-94 269
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26990.t1 AT1G75350 59.398 133 42 2 1 121 1 133 1.76e-52 162

Find 31 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAACCACATACTCTTTC+TGG 0.120517 1.3:+2489670 None:intergenic
TTAGCATCTTCGTAGAATTT+TGG 0.129378 1.3:+2489735 None:intergenic
CGGTGAATTGTCGCAGATTA+TGG 0.250630 1.3:-2489559 MS.gene26990:CDS
ATGGAGATTCCCGTGCTTAA+AGG 0.284752 1.3:-2489540 MS.gene26990:CDS
AGGTAACAGGTCTGCTCTTA+TGG 0.321423 1.3:-2489619 MS.gene26990:CDS
GACCTGTTACCTAGGTAAAA+TGG 0.333258 1.3:+2489630 None:intergenic
TTAATCCCTTTACGTCTAGA+AGG 0.369196 1.3:+2489504 None:intergenic
TTATTCCTTCTAGACGTAAA+GGG 0.415844 1.3:-2489509 MS.gene26990:CDS
ATCAACCACATACTCTTTCT+GGG 0.433365 1.3:+2489671 None:intergenic
ATAACCAATACCTTTGTTCA+AGG 0.436471 1.3:-2489972 MS.gene26990:CDS
GTTATTCCTTCTAGACGTAA+AGG 0.445368 1.3:-2489510 MS.gene26990:CDS
TATGTGGTTGATGTTTGGTC+TGG 0.461863 1.3:-2489660 MS.gene26990:CDS
TCTTATGGTGAGTGATGATC+AGG 0.479241 1.3:-2489604 MS.gene26990:CDS
AAGAGTATGTGGTTGATGTT+TGG 0.479372 1.3:-2489665 MS.gene26990:CDS
GAGAAGTTTCGTAAGAAGTT+CGG 0.493517 1.3:-2489579 MS.gene26990:CDS
AACGAGCTTGTGATGACAAC+TGG 0.499125 1.3:-2489699 MS.gene26990:CDS
ATGTGGTTGATGTTTGGTCT+GGG 0.499973 1.3:-2489659 MS.gene26990:CDS
TTTGTGGGAAGTGGAAGGAA+TGG 0.505762 1.3:+2489945 None:intergenic
AAAGGGATTAAAAGTGGTGG+TGG 0.510648 1.3:-2489492 MS.gene26990:CDS
AACAATCTCACCTTTAAGCA+CGG 0.530428 1.3:+2489530 None:intergenic
TAAGAGCAGACCTGTTACCT+AGG 0.535799 1.3:+2489622 None:intergenic
AAAATTCTACGAAGATGCTA+AGG 0.547901 1.3:-2489733 MS.gene26990:CDS
ACAATCTCACCTTTAAGCAC+GGG 0.566321 1.3:+2489531 None:intergenic
AGACGTAAAGGGATTAAAAG+TGG 0.579688 1.3:-2489498 MS.gene26990:CDS
GTTTGGTTGCAGAATCAACA+AGG 0.616245 1.3:-2489807 MS.gene26990:intron
AAGGGATTAAAAGTGGTGGT+GGG 0.621652 1.3:-2489491 MS.gene26990:CDS
CGTAAAGGGATTAAAAGTGG+TGG 0.637642 1.3:-2489495 MS.gene26990:CDS
TGTTACCTAGGTAAAATGGA+TGG 0.664203 1.3:+2489634 None:intergenic
TGGAACCCAGAAAGAGTATG+TGG 0.674951 1.3:-2489676 MS.gene26990:CDS
GAGCTTGTGATGACAACTGG+TGG 0.675964 1.3:-2489696 MS.gene26990:CDS
TATGGTGAGTGATGATCAGG+TGG 0.684535 1.3:-2489601 MS.gene26990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACAAAAAACATGT+TGG + chr1.3:2489617-2489636 None:intergenic 15.0%
!! GAAACAATAATATGAATAAT+TGG + chr1.3:2489581-2489600 None:intergenic 15.0%
!! ATGAATAATTGGAAATGATA+AGG + chr1.3:2489570-2489589 None:intergenic 20.0%
! AATCAACAAGGAAAAAAAGT+GGG - chr1.3:2489668-2489687 MS.gene26990:CDS 25.0%
AAAATTCTACGAAGATGCTA+AGG - chr1.3:2489730-2489749 MS.gene26990:CDS 30.0%
AATTGGAAATGATAAGGAAG+TGG + chr1.3:2489564-2489583 None:intergenic 30.0%
ATAACCAATACCTTTGTTCA+AGG - chr1.3:2489491-2489510 MS.gene26990:CDS 30.0%
GAATCAACAAGGAAAAAAAG+TGG - chr1.3:2489667-2489686 MS.gene26990:CDS 30.0%
TTATTCCTTCTAGACGTAAA+GGG - chr1.3:2489954-2489973 MS.gene26990:CDS 30.0%
! TAGCATCTTCGTAGAATTTT+GGG + chr1.3:2489730-2489749 None:intergenic 30.0%
! TTAGCATCTTCGTAGAATTT+TGG + chr1.3:2489731-2489750 None:intergenic 30.0%
! TTTTTGTGTGTGTTTGTGTT+TGG - chr1.3:2489639-2489658 MS.gene26990:CDS 30.0%
!! TTTTCCTTGAACAAAGGTAT+TGG + chr1.3:2489498-2489517 None:intergenic 30.0%
!!! TACTACAGTACGTTTTTTGT+GGG + chr1.3:2489536-2489555 None:intergenic 30.0%
!!! TTACTACAGTACGTTTTTTG+TGG + chr1.3:2489537-2489556 None:intergenic 30.0%
AACAATCTCACCTTTAAGCA+CGG + chr1.3:2489936-2489955 None:intergenic 35.0%
AGACGTAAAGGGATTAAAAG+TGG - chr1.3:2489965-2489984 MS.gene26990:CDS 35.0%
ATCAACCACATACTCTTTCT+GGG + chr1.3:2489795-2489814 None:intergenic 35.0%
GAGAAGTTTCGTAAGAAGTT+CGG - chr1.3:2489884-2489903 MS.gene26990:intron 35.0%
GGAAAAAAAGTGGGCAATTT+CGG - chr1.3:2489677-2489696 MS.gene26990:CDS 35.0%
GTTATTCCTTCTAGACGTAA+AGG - chr1.3:2489953-2489972 MS.gene26990:CDS 35.0%
TGTTACCTAGGTAAAATGGA+TGG + chr1.3:2489832-2489851 None:intergenic 35.0%
TTAATCCCTTTACGTCTAGA+AGG + chr1.3:2489962-2489981 None:intergenic 35.0%
! AAGAGTATGTGGTTGATGTT+TGG - chr1.3:2489798-2489817 MS.gene26990:CDS 35.0%
! ATCCATTTTACCTAGGTAAC+AGG - chr1.3:2489831-2489850 MS.gene26990:intron 35.0%
! GAATGGTTTTCCTTGAACAA+AGG + chr1.3:2489504-2489523 None:intergenic 35.0%
AAAGGGATTAAAAGTGGTGG+TGG - chr1.3:2489971-2489990 MS.gene26990:CDS 40.0%
AAGGGATTAAAAGTGGTGGT+GGG - chr1.3:2489972-2489991 MS.gene26990:CDS 40.0%
ACAATCTCACCTTTAAGCAC+GGG + chr1.3:2489935-2489954 None:intergenic 40.0%
CATCAACCACATACTCTTTC+TGG + chr1.3:2489796-2489815 None:intergenic 40.0%
CGTAAAGGGATTAAAAGTGG+TGG - chr1.3:2489968-2489987 MS.gene26990:CDS 40.0%
GACCTGTTACCTAGGTAAAA+TGG + chr1.3:2489836-2489855 None:intergenic 40.0%
GGAAATGATAAGGAAGTGGT+TGG + chr1.3:2489560-2489579 None:intergenic 40.0%
GTTTGGTTGCAGAATCAACA+AGG - chr1.3:2489656-2489675 MS.gene26990:CDS 40.0%
TCTTATGGTGAGTGATGATC+AGG - chr1.3:2489859-2489878 MS.gene26990:intron 40.0%
! ATGTGGTTGATGTTTGGTCT+GGG - chr1.3:2489804-2489823 MS.gene26990:intron 40.0%
! TATGTGGTTGATGTTTGGTC+TGG - chr1.3:2489803-2489822 MS.gene26990:intron 40.0%
!!! AGTACGTTTTTTGTGGGAAG+TGG + chr1.3:2489530-2489549 None:intergenic 40.0%
AACGAGCTTGTGATGACAAC+TGG - chr1.3:2489764-2489783 MS.gene26990:CDS 45.0%
AGGTAACAGGTCTGCTCTTA+TGG - chr1.3:2489844-2489863 MS.gene26990:intron 45.0%
ATGGAGATTCCCGTGCTTAA+AGG - chr1.3:2489923-2489942 MS.gene26990:intron 45.0%
CGGTGAATTGTCGCAGATTA+TGG - chr1.3:2489904-2489923 MS.gene26990:intron 45.0%
TAAGAGCAGACCTGTTACCT+AGG + chr1.3:2489844-2489863 None:intergenic 45.0%
TATGGTGAGTGATGATCAGG+TGG - chr1.3:2489862-2489881 MS.gene26990:intron 45.0%
TTTGTGGGAAGTGGAAGGAA+TGG + chr1.3:2489521-2489540 None:intergenic 45.0%
! GGGAACCATCCATTTTACCT+AGG - chr1.3:2489824-2489843 MS.gene26990:intron 45.0%
! TGGAACCCAGAAAGAGTATG+TGG - chr1.3:2489787-2489806 MS.gene26990:CDS 45.0%
!!! CGTTTTTTGTGGGAAGTGGA+AGG + chr1.3:2489526-2489545 None:intergenic 45.0%
GAGCTTGTGATGACAACTGG+TGG - chr1.3:2489767-2489786 MS.gene26990:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 2489479 2490006 2489479 ID=MS.gene26990
chr1.3 mRNA 2489479 2490006 2489479 ID=MS.gene26990.t1;Parent=MS.gene26990
chr1.3 exon 2489941 2490006 2489941 ID=MS.gene26990.t1.exon1;Parent=MS.gene26990.t1
chr1.3 CDS 2489941 2490006 2489941 ID=cds.MS.gene26990.t1;Parent=MS.gene26990.t1
chr1.3 exon 2489479 2489817 2489479 ID=MS.gene26990.t1.exon2;Parent=MS.gene26990.t1
chr1.3 CDS 2489479 2489817 2489479 ID=cds.MS.gene26990.t1;Parent=MS.gene26990.t1
Gene Sequence

>MS.gene26990

ATGGCGCAGTGCATAACCAATACCTTTGTTCAAGGAAAACCATTCCTTCCACTTCCCACAAAAAACGTACTGTAGTAACCAACCACTTCCTTATCATTTCCAATTATTCATATTATTGTTTCTGTATTAAACTAACCAACATGTTTTTTGTTTTTTTTTTTTTTTGTGTGTGTTTGTGTTTGGTTGCAGAATCAACAAGGAAAAAAAGTGGGCAATTTCGGAGTGTATTGCAAGAAGAAAGACATACACCCAAAATTCTACGAAGATGCTAAGGTTTACTGTAACAACGAGCTTGTGATGACAACTGGTGGAACCCAGAAAGAGTATGTGGTTGATGTTTGGTCTGGGAACCATCCATTTTACCTAGGTAACAGGTCTGCTCTTATGGTGAGTGATGATCAGGTGGAGAAGTTTCGTAAGAAGTTCGGTGAATTGTCGCAGATTATGGAGATTCCCGTGCTTAAAGGTGAGATTGTTATTCCTTCTAGACGTAAAGGGATTAAAAGTGGTGGTGGGAAGAAGAAATGA

Protein sequence

>MS.gene26990.t1

MAQCITNTFVQGKPFLPLPTKNNQQGKKVGNFGVYCKKKDIHPKFYEDAKVYCNNELVMTTGGTQKEYVVDVWSGNHPFYLGNRSALMVSDDQVEKFRKKFGELSQIMEIPVLKGEIVIPSRRKGIKSGGGKKK