Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34231.t1 | XP_003628554.1 | 95.6 | 159 | 7 | 0 | 1 | 159 | 15 | 173 | 2.20E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34231.t1 | Q9ASV6 | 67.6 | 148 | 44 | 2 | 8 | 153 | 42 | 187 | 8.1e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34231.t1 | G7LDS2 | 95.6 | 159 | 7 | 0 | 1 | 159 | 15 | 173 | 1.6e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene34231 | 0.830278 | 3.21E-55 | -1.69E-46 |
| MS.gene049351 | MS.gene34231 | 0.855604 | 5.64E-62 | -1.69E-46 |
| MS.gene049389 | MS.gene34231 | 0.860158 | 2.51E-63 | -1.69E-46 |
| MS.gene049427 | MS.gene34231 | 0.846684 | 1.86E-59 | -1.69E-46 |
| MS.gene049491 | MS.gene34231 | 0.873975 | 9.69E-68 | -1.69E-46 |
| MS.gene049614 | MS.gene34231 | 0.864433 | 1.22E-64 | -1.69E-46 |
| MS.gene049661 | MS.gene34231 | 0.856377 | 3.35E-62 | -1.69E-46 |
| MS.gene049662 | MS.gene34231 | 0.848129 | 7.46E-60 | -1.69E-46 |
| MS.gene049758 | MS.gene34231 | 0.865805 | 4.52E-65 | -1.69E-46 |
| MS.gene049760 | MS.gene34231 | 0.833606 | 4.84E-56 | -1.69E-46 |
| MS.gene049805 | MS.gene34231 | 0.811342 | 7.34E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene34231 | 0.892998 | 9.46E-75 | -1.69E-46 |
| MS.gene050099 | MS.gene34231 | 0.861996 | 6.92E-64 | -1.69E-46 |
| MS.gene050128 | MS.gene34231 | 0.862604 | 4.51E-64 | -1.69E-46 |
| MS.gene050301 | MS.gene34231 | 0.898008 | 8.08E-77 | -1.69E-46 |
| MS.gene050397 | MS.gene34231 | 0.849744 | 2.66E-60 | -1.69E-46 |
| MS.gene050404 | MS.gene34231 | 0.841824 | 3.76E-58 | -1.69E-46 |
| MS.gene050406 | MS.gene34231 | 0.836182 | 1.09E-56 | -1.69E-46 |
| MS.gene050432 | MS.gene34231 | 0.865284 | 6.60E-65 | -1.69E-46 |
| MS.gene050464 | MS.gene34231 | 0.852568 | 4.24E-61 | -1.69E-46 |
| MS.gene050529 | MS.gene34231 | 0.87329 | 1.65E-67 | -1.69E-46 |
| MS.gene05053 | MS.gene34231 | 0.808296 | 3.32E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene34231 | 0.812686 | 3.74E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene34231 | 0.89426 | 2.92E-75 | -1.69E-46 |
| MS.gene050770 | MS.gene34231 | 0.896624 | 3.09E-76 | -1.69E-46 |
| MS.gene050783 | MS.gene34231 | 0.819497 | 1.13E-52 | -1.69E-46 |
| MS.gene050812 | MS.gene34231 | 0.823841 | 1.11E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene34231 | 0.924548 | 6.08E-90 | -1.69E-46 |
| MS.gene05105 | MS.gene34231 | 0.822561 | 2.22E-53 | -1.69E-46 |
| MS.gene051228 | MS.gene34231 | 0.830134 | 3.48E-55 | -1.69E-46 |
| MS.gene051471 | MS.gene34231 | 0.906055 | 2.23E-80 | -1.69E-46 |
| MS.gene051662 | MS.gene34231 | 0.822075 | 2.88E-53 | -1.69E-46 |
| MS.gene051663 | MS.gene34231 | 0.823127 | 1.64E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene34231 | 0.818498 | 1.90E-52 | -1.69E-46 |
| MS.gene051692 | MS.gene34231 | 0.817339 | 3.47E-52 | -1.69E-46 |
| MS.gene051877 | MS.gene34231 | 0.821666 | 3.58E-53 | -1.69E-46 |
| MS.gene052078 | MS.gene34231 | 0.91418 | 2.59E-84 | -1.69E-46 |
| MS.gene052178 | MS.gene34231 | 0.874506 | 6.40E-68 | -1.69E-46 |
| MS.gene052188 | MS.gene34231 | 0.867755 | 1.08E-65 | -1.69E-46 |
| MS.gene052207 | MS.gene34231 | 0.806676 | 7.33E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene34231 | 0.80734 | 5.30E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene34231 | 0.817422 | 3.33E-52 | -1.69E-46 |
| MS.gene052287 | MS.gene34231 | 0.824008 | 1.02E-53 | -1.69E-46 |
| MS.gene052290 | MS.gene34231 | 0.880225 | 6.54E-70 | -1.69E-46 |
| MS.gene052293 | MS.gene34231 | 0.81893 | 1.52E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene34231 | 0.893502 | 5.92E-75 | -1.69E-46 |
| MS.gene052322 | MS.gene34231 | 0.823944 | 1.05E-53 | -1.69E-46 |
| MS.gene052326 | MS.gene34231 | 0.900924 | 4.50E-78 | -1.69E-46 |
| MS.gene052340 | MS.gene34231 | 0.855094 | 7.95E-62 | -1.69E-46 |
| MS.gene052379 | MS.gene34231 | 0.828935 | 6.82E-55 | -1.69E-46 |
| MS.gene052484 | MS.gene34231 | 0.816342 | 5.81E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene34231 | 0.819822 | 9.49E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene34231 | 0.818735 | 1.68E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene34231 | 0.851259 | 9.97E-61 | -1.69E-46 |
| MS.gene053104 | MS.gene34231 | 0.827345 | 1.65E-54 | -1.69E-46 |
| MS.gene053181 | MS.gene34231 | 0.824312 | 8.64E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene34231 | 0.875629 | 2.65E-68 | -1.69E-46 |
| MS.gene053322 | MS.gene34231 | 0.87917 | 1.55E-69 | -1.69E-46 |
| MS.gene053384 | MS.gene34231 | 0.808896 | 2.47E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene34231 | 0.859906 | 2.99E-63 | -1.69E-46 |
| MS.gene053559 | MS.gene34231 | 0.805371 | 1.38E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene34231 | 0.861239 | 1.18E-63 | -1.69E-46 |
| MS.gene053907 | MS.gene34231 | 0.812211 | 4.75E-51 | -1.69E-46 |
| MS.gene053928 | MS.gene34231 | 0.805722 | 1.16E-49 | -1.69E-46 |
| MS.gene053971 | MS.gene34231 | 0.802995 | 4.31E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene34231 | 0.886036 | 4.85E-72 | -1.69E-46 |
| MS.gene054376 | MS.gene34231 | 0.901415 | 2.74E-78 | -1.69E-46 |
| MS.gene05455 | MS.gene34231 | 0.860299 | 2.28E-63 | -1.69E-46 |
| MS.gene054802 | MS.gene34231 | 0.871131 | 8.63E-67 | -1.69E-46 |
| MS.gene054803 | MS.gene34231 | 0.869923 | 2.15E-66 | -1.69E-46 |
| MS.gene054804 | MS.gene34231 | 0.873659 | 1.24E-67 | -1.69E-46 |
| MS.gene054955 | MS.gene34231 | 0.834097 | 3.65E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene34231 | 0.840737 | 7.26E-58 | -1.69E-46 |
| MS.gene055254 | MS.gene34231 | 0.887986 | 8.81E-73 | -1.69E-46 |
| MS.gene055277 | MS.gene34231 | 0.897568 | 1.24E-76 | -1.69E-46 |
| MS.gene055390 | MS.gene34231 | 0.888658 | 4.86E-73 | -1.69E-46 |
| MS.gene055397 | MS.gene34231 | 0.872467 | 3.11E-67 | -1.69E-46 |
| MS.gene055412 | MS.gene34231 | 0.840684 | 7.50E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene34231 | 0.901061 | 3.92E-78 | -1.69E-46 |
| MS.gene055428 | MS.gene34231 | 0.875153 | 3.86E-68 | -1.69E-46 |
| MS.gene055499 | MS.gene34231 | 0.867238 | 1.59E-65 | -1.69E-46 |
| MS.gene055533 | MS.gene34231 | 0.802937 | 4.43E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene34231 | 0.8102 | 1.30E-50 | -1.69E-46 |
| MS.gene055647 | MS.gene34231 | 0.815943 | 7.13E-52 | -1.69E-46 |
| MS.gene055712 | MS.gene34231 | 0.832318 | 1.01E-55 | -1.69E-46 |
| MS.gene055896 | MS.gene34231 | 0.867572 | 1.24E-65 | -1.69E-46 |
| MS.gene05592 | MS.gene34231 | 0.828983 | 6.64E-55 | -1.69E-46 |
| MS.gene055930 | MS.gene34231 | 0.801125 | 1.04E-48 | -1.69E-46 |
| MS.gene056191 | MS.gene34231 | 0.851174 | 1.05E-60 | -1.69E-46 |
| MS.gene056314 | MS.gene34231 | 0.808101 | 3.65E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene34231 | 0.848069 | 7.75E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene34231 | 0.86774 | 1.10E-65 | -1.69E-46 |
| MS.gene056609 | MS.gene34231 | 0.906689 | 1.13E-80 | -1.69E-46 |
| MS.gene056612 | MS.gene34231 | 0.87918 | 1.54E-69 | -1.69E-46 |
| MS.gene056719 | MS.gene34231 | 0.807683 | 4.48E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene34231 | 0.841836 | 3.73E-58 | -1.69E-46 |
| MS.gene056897 | MS.gene34231 | 0.830303 | 3.17E-55 | -1.69E-46 |
| MS.gene056931 | MS.gene34231 | 0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene056933 | MS.gene34231 | 0.812953 | 3.27E-51 | -1.69E-46 |
| MS.gene056935 | MS.gene34231 | 0.806874 | 6.66E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene34231 | 0.837354 | 5.46E-57 | -1.69E-46 |
| MS.gene05702 | MS.gene34231 | 0.844477 | 7.38E-59 | -1.69E-46 |
| MS.gene057121 | MS.gene34231 | 0.815725 | 7.98E-52 | -1.69E-46 |
| MS.gene057123 | MS.gene34231 | 0.841181 | 5.55E-58 | -1.69E-46 |
| MS.gene057124 | MS.gene34231 | 0.800793 | 1.22E-48 | -1.69E-46 |
| MS.gene057129 | MS.gene34231 | 0.8804 | 5.66E-70 | -1.69E-46 |
| MS.gene057146 | MS.gene34231 | 0.825519 | 4.49E-54 | -1.69E-46 |
| MS.gene057245 | MS.gene34231 | 0.821927 | 3.11E-53 | -1.69E-46 |
| MS.gene057295 | MS.gene34231 | 0.90842 | 1.74E-81 | -1.69E-46 |
| MS.gene05733 | MS.gene34231 | 0.865275 | 6.65E-65 | -1.69E-46 |
| MS.gene05735 | MS.gene34231 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene057439 | MS.gene34231 | 0.861806 | 7.92E-64 | -1.69E-46 |
| MS.gene057448 | MS.gene34231 | 0.830797 | 2.40E-55 | -1.69E-46 |
| MS.gene057449 | MS.gene34231 | 0.827924 | 1.20E-54 | -1.69E-46 |
| MS.gene057450 | MS.gene34231 | 0.846912 | 1.61E-59 | -1.69E-46 |
| MS.gene057796 | MS.gene34231 | 0.81173 | 6.05E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene34231 | 0.863233 | 2.88E-64 | -1.69E-46 |
| MS.gene058019 | MS.gene34231 | 0.816743 | 4.72E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene34231 | 0.869564 | 2.82E-66 | -1.69E-46 |
| MS.gene05811 | MS.gene34231 | 0.86823 | 7.62E-66 | -1.69E-46 |
| MS.gene058233 | MS.gene34231 | 0.850035 | 2.20E-60 | -1.69E-46 |
| MS.gene058276 | MS.gene34231 | 0.826836 | 2.18E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene34231 | 0.91306 | 9.53E-84 | -1.69E-46 |
| MS.gene058365 | MS.gene34231 | 0.843016 | 1.82E-58 | -1.69E-46 |
| MS.gene058537 | MS.gene34231 | -0.817323 | 3.50E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene34231 | 0.924052 | 1.18E-89 | -1.69E-46 |
| MS.gene058748 | MS.gene34231 | 0.813115 | 3.01E-51 | -1.69E-46 |
| MS.gene058787 | MS.gene34231 | 0.872664 | 2.67E-67 | -1.69E-46 |
| MS.gene058845 | MS.gene34231 | 0.845631 | 3.60E-59 | -1.69E-46 |
| MS.gene058903 | MS.gene34231 | 0.812151 | 4.90E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene34231 | 0.840807 | 6.96E-58 | -1.69E-46 |
| MS.gene058993 | MS.gene34231 | 0.811555 | 6.60E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene34231 | 0.867874 | 9.92E-66 | -1.69E-46 |
| MS.gene059228 | MS.gene34231 | 0.818021 | 2.44E-52 | -1.69E-46 |
| MS.gene059294 | MS.gene34231 | 0.816226 | 6.17E-52 | -1.69E-46 |
| MS.gene059326 | MS.gene34231 | 0.880859 | 3.88E-70 | -1.69E-46 |
| MS.gene059342 | MS.gene34231 | 0.849563 | 2.98E-60 | -1.69E-46 |
| MS.gene059366 | MS.gene34231 | 0.804043 | 2.61E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene34231 | 0.858122 | 1.02E-62 | -1.69E-46 |
| MS.gene059536 | MS.gene34231 | 0.863985 | 1.68E-64 | -1.69E-46 |
| MS.gene059597 | MS.gene34231 | 0.89545 | 9.48E-76 | -1.69E-46 |
| MS.gene05959 | MS.gene34231 | 0.859131 | 5.11E-63 | -1.69E-46 |
| MS.gene059676 | MS.gene34231 | 0.8964 | 3.83E-76 | -1.69E-46 |
| MS.gene059688 | MS.gene34231 | 0.876861 | 9.97E-69 | -1.69E-46 |
| MS.gene059692 | MS.gene34231 | 0.898056 | 7.71E-77 | -1.69E-46 |
| MS.gene059695 | MS.gene34231 | 0.89576 | 7.05E-76 | -1.69E-46 |
| MS.gene059708 | MS.gene34231 | 0.858077 | 1.06E-62 | -1.69E-46 |
| MS.gene05984 | MS.gene34231 | 0.843351 | 1.48E-58 | -1.69E-46 |
| MS.gene059858 | MS.gene34231 | 0.814287 | 1.66E-51 | -1.69E-46 |
| MS.gene059894 | MS.gene34231 | 0.903519 | 3.19E-79 | -1.69E-46 |
| MS.gene059899 | MS.gene34231 | 0.808029 | 3.79E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene34231 | 0.891741 | 3.01E-74 | -1.69E-46 |
| MS.gene059974 | MS.gene34231 | 0.840333 | 9.26E-58 | -1.69E-46 |
| MS.gene060005 | MS.gene34231 | 0.853029 | 3.13E-61 | -1.69E-46 |
| MS.gene06037 | MS.gene34231 | 0.87136 | 7.25E-67 | -1.69E-46 |
| MS.gene060407 | MS.gene34231 | 0.82896 | 6.72E-55 | -1.69E-46 |
| MS.gene060476 | MS.gene34231 | 0.804209 | 2.41E-49 | -1.69E-46 |
| MS.gene060496 | MS.gene34231 | 0.801975 | 6.99E-49 | -1.69E-46 |
| MS.gene060559 | MS.gene34231 | 0.819395 | 1.19E-52 | -1.69E-46 |
| MS.gene060800 | MS.gene34231 | 0.808749 | 2.66E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene34231 | 0.831506 | 1.61E-55 | -1.69E-46 |
| MS.gene060818 | MS.gene34231 | 0.842192 | 3.00E-58 | -1.69E-46 |
| MS.gene06090 | MS.gene34231 | 0.817524 | 3.15E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene34231 | 0.830376 | 3.04E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene34231 | 0.890935 | 6.28E-74 | -1.69E-46 |
| MS.gene061051 | MS.gene34231 | -0.876446 | 1.39E-68 | -1.69E-46 |
| MS.gene061112 | MS.gene34231 | 0.814992 | 1.16E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene34231 | 0.881062 | 3.28E-70 | -1.69E-46 |
| MS.gene061265 | MS.gene34231 | 0.810084 | 1.37E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34231.t1 | MTR_8g061350 | 95.597 | 159 | 7 | 0 | 1 | 159 | 15 | 173 | 4.81e-99 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34231.t1 | AT3G15190 | 77.876 | 113 | 23 | 1 | 43 | 153 | 75 | 187 | 8.95e-57 | 176 |
Find 42 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTACTGCCTTCTTTCTTC+TGG | 0.196704 | 8.4:-47617171 | None:intergenic |
| ATGGCGGAATCAACCTTCTT+TGG | 0.235795 | 8.4:-47615918 | None:intergenic |
| CCTAACTTCATCATCAAATT+CGG | 0.266189 | 8.4:+47615785 | MS.gene34231:CDS |
| CCGAATTTGATGATGAAGTT+AGG | 0.293575 | 8.4:-47615785 | None:intergenic |
| CTCTAGACTGGGAAATGTTT+CGG | 0.313050 | 8.4:-47615824 | None:intergenic |
| TCTAGACTGGGAAATGTTTC+GGG | 0.313830 | 8.4:-47615823 | None:intergenic |
| TTACAGTTACGGTACCTTCT+TGG | 0.353552 | 8.4:-47616012 | None:intergenic |
| CTTGGTACGGGTTCTAATCT+CGG | 0.388971 | 8.4:-47615994 | None:intergenic |
| CGCCATTAAGAGAGCTCGTC+AGG | 0.398567 | 8.4:+47615935 | MS.gene34231:CDS |
| GCCTCAATTGAGAAGATGAT+TGG | 0.400874 | 8.4:+47617065 | MS.gene34231:CDS |
| CTAACTTCATCATCAAATTC+GGG | 0.410171 | 8.4:+47615786 | MS.gene34231:CDS |
| CGAATTTGATGATGAAGTTA+GGG | 0.438285 | 8.4:-47615784 | None:intergenic |
| CAGACACTGGCTTGAGGCAC+AGG | 0.442721 | 8.4:-47617215 | None:intergenic |
| AGGTATGTTATCTTTGAATA+CGG | 0.445037 | 8.4:+47615857 | MS.gene34231:CDS |
| AGCCTGACGAGCTCTCTTAA+TGG | 0.465591 | 8.4:-47615937 | None:intergenic |
| CTTGTGAAAGGACTCTAGAC+TGG | 0.483441 | 8.4:-47615836 | None:intergenic |
| GTTACGGTACCTTCTTGGTA+CGG | 0.485051 | 8.4:-47616007 | None:intergenic |
| TTGCGAGGCTGCTCCAAAGA+AGG | 0.502462 | 8.4:+47615905 | MS.gene34231:CDS |
| CAGGGGCATACCAGCCATGA+TGG | 0.502689 | 8.4:-47617196 | None:intergenic |
| TCCAATCATCTTCTCAATTG+AGG | 0.507360 | 8.4:-47617066 | None:intergenic |
| TTACGGTACCTTCTTGGTAC+GGG | 0.515322 | 8.4:-47616006 | None:intergenic |
| TTGATGATGAAGTTAGGGTA+AGG | 0.521257 | 8.4:-47615779 | None:intergenic |
| AATTGACAAAGCAGTGAAAG+TGG | 0.540256 | 8.4:+47617103 | MS.gene34231:CDS |
| CAGTAGAGATCCATCATGGC+TGG | 0.548246 | 8.4:+47617186 | MS.gene34231:CDS |
| TCGGCTTGCCAGAAGAAAGA+AGG | 0.559181 | 8.4:+47617163 | MS.gene34231:CDS |
| CAGGAGCAAATCGCAAGTCT+CGG | 0.565582 | 8.4:+47617144 | MS.gene34231:CDS |
| AAGATAACATACCTTGTGAA+AGG | 0.576623 | 8.4:-47615848 | None:intergenic |
| AGACACTGGCTTGAGGCACA+GGG | 0.582207 | 8.4:-47617214 | None:intergenic |
| CAGTCTAGAGTCCTTTCACA+AGG | 0.588356 | 8.4:+47615837 | MS.gene34231:CDS |
| ATATAAAAGGATTCAGACAC+TGG | 0.591833 | 8.4:-47617228 | None:intergenic |
| AGAGAGCTCGTCAGGCTGAG+AGG | 0.604628 | 8.4:+47615943 | MS.gene34231:CDS |
| GATTAGAACCCGTACCAAGA+AGG | 0.607531 | 8.4:+47615998 | MS.gene34231:CDS |
| ATTGACAAAGCAGTGAAAGT+GGG | 0.623402 | 8.4:+47617104 | MS.gene34231:CDS |
| TTGTGAAAGGACTCTAGACT+GGG | 0.643097 | 8.4:-47615835 | None:intergenic |
| TTGAATACGGTGCAGAAGCG+TGG | 0.643767 | 8.4:+47615870 | MS.gene34231:CDS |
| AAGGCAGTAGAGATCCATCA+TGG | 0.646488 | 8.4:+47617182 | MS.gene34231:CDS |
| TGGTGTTGTTGTTGTTTGCG+AGG | 0.647337 | 8.4:+47615890 | MS.gene34231:CDS |
| GGAACTTTGCATAGAAACAC+AGG | 0.653414 | 8.4:+47617125 | MS.gene34231:CDS |
| AGGATTCAGACACTGGCTTG+AGG | 0.656971 | 8.4:-47617221 | None:intergenic |
| AATTGAGAAGATGATTGGAG+AGG | 0.673219 | 8.4:+47617070 | MS.gene34231:CDS |
| CTGACGAGCTCTCTTAATGG+CGG | 0.689854 | 8.4:-47615934 | None:intergenic |
| GACACTGGCTTGAGGCACAG+GGG | 0.714773 | 8.4:-47617213 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCATTTTCTCAAATTATTAT+TGG | + | chr8.4:47616075-47616094 | MS.gene34231:intron | 15.0% |
| !! | AGAAATAGATGATAATATGA+TGG | + | chr8.4:47616504-47616523 | MS.gene34231:intron | 20.0% |
| !! | ATAGAATAGCTATAATAACA+TGG | + | chr8.4:47616164-47616183 | MS.gene34231:intron | 20.0% |
| !! | TTATATGAGTTACACATTAT+GGG | - | chr8.4:47616430-47616449 | None:intergenic | 20.0% |
| !!! | ATTGATAATATGCAAGAAAA+GGG | + | chr8.4:47616473-47616492 | MS.gene34231:intron | 20.0% |
| !!! | GAAAACATGATTTGTATTTT+AGG | + | chr8.4:47616340-47616359 | MS.gene34231:intron | 20.0% |
| ! | AGGTATGTTATCTTTGAATA+CGG | + | chr8.4:47615857-47615876 | MS.gene34231:CDS | 25.0% |
| ! | GTTATATGAGTTACACATTA+TGG | - | chr8.4:47616431-47616450 | None:intergenic | 25.0% |
| ! | TAACATTCCTTGAGAATATT+TGG | + | chr8.4:47616447-47616466 | MS.gene34231:intron | 25.0% |
| ! | TAATTATATAACTTGAGCGA+AGG | - | chr8.4:47616290-47616309 | None:intergenic | 25.0% |
| ! | TCTCAAATTATTATTGGTGT+CGG | + | chr8.4:47616081-47616100 | MS.gene34231:intron | 25.0% |
| ! | TCTTACTTTCAATTGTTTGT+AGG | + | chr8.4:47616986-47617005 | MS.gene34231:intron | 25.0% |
| !! | AAAATTGATTCCATAGCTTT+AGG | + | chr8.4:47616233-47616252 | MS.gene34231:intron | 25.0% |
| !! | AAATTGATTCCATAGCTTTA+GGG | + | chr8.4:47616234-47616253 | MS.gene34231:intron | 25.0% |
| !! | AATAGTTCAAGTTACAGTTA+CGG | - | chr8.4:47616026-47616045 | None:intergenic | 25.0% |
| !! | ATGATAATATGATGGAGTAT+GGG | + | chr8.4:47616512-47616531 | MS.gene34231:intron | 25.0% |
| !! | CATTGATAATATGCAAGAAA+AGG | + | chr8.4:47616472-47616491 | MS.gene34231:intron | 25.0% |
| !! | CTTTTCTTGCATATTATCAA+TGG | - | chr8.4:47616474-47616493 | None:intergenic | 25.0% |
| !! | TTTCAATTGTTTGTAGGTTT+TGG | + | chr8.4:47616992-47617011 | MS.gene34231:intron | 25.0% |
| !!! | CATGTTTTCAAGATGAAATT+GGG | - | chr8.4:47616329-47616348 | None:intergenic | 25.0% |
| !!! | TCATGTTTTCAAGATGAAAT+TGG | - | chr8.4:47616330-47616349 | None:intergenic | 25.0% |
| AAGATAACATACCTTGTGAA+AGG | - | chr8.4:47615851-47615870 | None:intergenic | 30.0% | |
| ATAGCTATTCTATGAAGAAC+TGG | - | chr8.4:47616157-47616176 | None:intergenic | 30.0% | |
| CAGAAACTAGAACTAAAACA+TGG | - | chr8.4:47616677-47616696 | None:intergenic | 30.0% | |
| CCTAACTTCATCATCAAATT+CGG | + | chr8.4:47615785-47615804 | MS.gene34231:CDS | 30.0% | |
| CTAACTTCATCATCAAATTC+GGG | + | chr8.4:47615786-47615805 | MS.gene34231:CDS | 30.0% | |
| TGACAATTCAACAGTTTAGA+AGG | + | chr8.4:47616643-47616662 | MS.gene34231:intron | 30.0% | |
| TGCAAATAAAACTACACGTT+GGG | - | chr8.4:47616948-47616967 | None:intergenic | 30.0% | |
| ! | ATCATCAGAAAATGTTGCTT+CGG | - | chr8.4:47616568-47616587 | None:intergenic | 30.0% |
| ! | GATGATAATATGATGGAGTA+TGG | + | chr8.4:47616511-47616530 | MS.gene34231:intron | 30.0% |
| !! | CGAATTTGATGATGAAGTTA+GGG | - | chr8.4:47615787-47615806 | None:intergenic | 30.0% |
| AAAAACAAGAACACAACACC+TGG | - | chr8.4:47616374-47616393 | None:intergenic | 35.0% | |
| AAAACAAGAACACAACACCT+GGG | - | chr8.4:47616373-47616392 | None:intergenic | 35.0% | |
| AACAATAAGAATCCCTGACA+TGG | + | chr8.4:47616834-47616853 | MS.gene34231:intron | 35.0% | |
| AATTGACAAAGCAGTGAAAG+TGG | + | chr8.4:47617103-47617122 | MS.gene34231:CDS | 35.0% | |
| AATTGAGAAGATGATTGGAG+AGG | + | chr8.4:47617070-47617089 | MS.gene34231:CDS | 35.0% | |
| ATGAAATTGGGTGATGACAA+TGG | - | chr8.4:47616317-47616336 | None:intergenic | 35.0% | |
| ATTGACAAAGCAGTGAAAGT+GGG | + | chr8.4:47617104-47617123 | MS.gene34231:CDS | 35.0% | |
| CAATGGTCCAAATATTCTCA+AGG | - | chr8.4:47616457-47616476 | None:intergenic | 35.0% | |
| CTGCAAATAAAACTACACGT+TGG | - | chr8.4:47616949-47616968 | None:intergenic | 35.0% | |
| GCAAATAAAACTACACGTTG+GGG | - | chr8.4:47616947-47616966 | None:intergenic | 35.0% | |
| GTAGCTGATGTTATAATTGC+AGG | + | chr8.4:47616856-47616875 | MS.gene34231:intron | 35.0% | |
| GTGGAGTTTCATTATCTTAC+AGG | - | chr8.4:47616813-47616832 | None:intergenic | 35.0% | |
| TCAGGGATTCTTATTGTTTG+TGG | - | chr8.4:47616832-47616851 | None:intergenic | 35.0% | |
| TCCAATCATCTTCTCAATTG+AGG | - | chr8.4:47617069-47617088 | None:intergenic | 35.0% | |
| !! | CCGAATTTGATGATGAAGTT+AGG | - | chr8.4:47615788-47615807 | None:intergenic | 35.0% |
| !! | TTGATGATGAAGTTAGGGTA+AGG | - | chr8.4:47615782-47615801 | None:intergenic | 35.0% |
| !!! | CAACGTGTAGTTTTATTTGC+AGG | + | chr8.4:47616947-47616966 | MS.gene34231:intron | 35.0% |
| AGAGGGAAGTTTGAGTAACA+TGG | + | chr8.4:47616260-47616279 | MS.gene34231:intron | 40.0% | |
| ATAAAACTACACGTTGGGGT+TGG | - | chr8.4:47616943-47616962 | None:intergenic | 40.0% | |
| ATAACATCAGCTACCATGTC+AGG | - | chr8.4:47616850-47616869 | None:intergenic | 40.0% | |
| CTCTAGACTGGGAAATGTTT+CGG | - | chr8.4:47615827-47615846 | None:intergenic | 40.0% | |
| GCCTCAATTGAGAAGATGAT+TGG | + | chr8.4:47617065-47617084 | MS.gene34231:CDS | 40.0% | |
| GGAACTTTGCATAGAAACAC+AGG | + | chr8.4:47617125-47617144 | MS.gene34231:CDS | 40.0% | |
| TAACATCAGCTACCATGTCA+GGG | - | chr8.4:47616849-47616868 | None:intergenic | 40.0% | |
| TCTAGACTGGGAAATGTTTC+GGG | - | chr8.4:47615826-47615845 | None:intergenic | 40.0% | |
| TTACAGTTACGGTACCTTCT+TGG | - | chr8.4:47616015-47616034 | None:intergenic | 40.0% | |
| TTGTGAAAGGACTCTAGACT+GGG | - | chr8.4:47615838-47615857 | None:intergenic | 40.0% | |
| ! | ATGATGGAGTATGGGATAGT+GGG | + | chr8.4:47616520-47616539 | MS.gene34231:intron | 40.0% |
| ! | TATGATGGAGTATGGGATAG+TGG | + | chr8.4:47616519-47616538 | MS.gene34231:intron | 40.0% |
| !! | CCATAGCTTTAGGGATTAGA+GGG | + | chr8.4:47616243-47616262 | MS.gene34231:intron | 40.0% |
| !! | TATTATTGGTGTCGGCATGT+TGG | + | chr8.4:47616089-47616108 | MS.gene34231:intron | 40.0% |
| !! | TCCATAGCTTTAGGGATTAG+AGG | + | chr8.4:47616242-47616261 | MS.gene34231:intron | 40.0% |
| !! | TGTTTCAAGTTACTGAACCC+TGG | + | chr8.4:47616588-47616607 | MS.gene34231:intron | 40.0% |
| !!! | TTTGTAGGTTTTGGAAGCAC+TGG | + | chr8.4:47617001-47617020 | MS.gene34231:intron | 40.0% |
| AAGGCAGTAGAGATCCATCA+TGG | + | chr8.4:47617182-47617201 | MS.gene34231:CDS | 45.0% | |
| CCCTCTAATCCCTAAAGCTA+TGG | - | chr8.4:47616246-47616265 | None:intergenic | 45.0% | |
| CTCTACTGCCTTCTTTCTTC+TGG | - | chr8.4:47617174-47617193 | None:intergenic | 45.0% | |
| CTTGTGAAAGGACTCTAGAC+TGG | - | chr8.4:47615839-47615858 | None:intergenic | 45.0% | |
| GATTAGAACCCGTACCAAGA+AGG | + | chr8.4:47615998-47616017 | MS.gene34231:CDS | 45.0% | |
| GTTACGGTACCTTCTTGGTA+CGG | - | chr8.4:47616010-47616029 | None:intergenic | 45.0% | |
| TTACGGTACCTTCTTGGTAC+GGG | - | chr8.4:47616009-47616028 | None:intergenic | 45.0% | |
| ! | ATGGCGGAATCAACCTTCTT+TGG | - | chr8.4:47615921-47615940 | None:intergenic | 45.0% |
| ! | CAGTCTAGAGTCCTTTCACA+AGG | + | chr8.4:47615837-47615856 | MS.gene34231:CDS | 45.0% |
| ! | CTTGGTACGGGTTCTAATCT+CGG | - | chr8.4:47615997-47616016 | None:intergenic | 45.0% |
| ! | TTCAAGTTACTGAACCCTGG+AGG | + | chr8.4:47616591-47616610 | MS.gene34231:intron | 45.0% |
| !! | GTATTTTAGGTGTCAGACCC+AGG | + | chr8.4:47616353-47616372 | MS.gene34231:intron | 45.0% |
| !! | TGGGATAGTGGGAGAGAAAA+TGG | + | chr8.4:47616531-47616550 | MS.gene34231:intron | 45.0% |
| !! | TGGTGTTGTTGTTGTTTGCG+AGG | + | chr8.4:47615890-47615909 | MS.gene34231:CDS | 45.0% |
| ACTGGCACAAACACAGACAC+CGG | - | chr8.4:47616139-47616158 | None:intergenic | 50.0% | |
| AGCCTGACGAGCTCTCTTAA+TGG | - | chr8.4:47615940-47615959 | None:intergenic | 50.0% | |
| AGGCGCAAATTAGGTGGTAC+TGG | + | chr8.4:47616611-47616630 | MS.gene34231:intron | 50.0% | |
| CAGGAGCAAATCGCAAGTCT+CGG | + | chr8.4:47617144-47617163 | MS.gene34231:CDS | 50.0% | |
| CAGTAGAGATCCATCATGGC+TGG | + | chr8.4:47617186-47617205 | MS.gene34231:CDS | 50.0% | |
| CTGACGAGCTCTCTTAATGG+CGG | - | chr8.4:47615937-47615956 | None:intergenic | 50.0% | |
| TTGAATACGGTGCAGAAGCG+TGG | + | chr8.4:47615870-47615889 | MS.gene34231:CDS | 50.0% | |
| ! | TCGGCTTGCCAGAAGAAAGA+AGG | + | chr8.4:47617163-47617182 | MS.gene34231:CDS | 50.0% |
| ACAGACACCGGACACAACAC+TGG | - | chr8.4:47616127-47616146 | None:intergenic | 55.0% | |
| CGCCATTAAGAGAGCTCGTC+AGG | + | chr8.4:47615935-47615954 | MS.gene34231:CDS | 55.0% | |
| TTGCGAGGCTGCTCCAAAGA+AGG | + | chr8.4:47615905-47615924 | MS.gene34231:CDS | 55.0% | |
| ! | ACCACCTAATTTGCGCCTCC+AGG | - | chr8.4:47616609-47616628 | None:intergenic | 55.0% |
| ! | CCACCTAATTTGCGCCTCCA+GGG | - | chr8.4:47616608-47616627 | None:intergenic | 55.0% |
| ! | GAACCCTGGAGGCGCAAATT+AGG | + | chr8.4:47616602-47616621 | MS.gene34231:intron | 55.0% |
| !! | AGACACTGGCTTGAGGCACA+GGG | - | chr8.4:47617217-47617236 | None:intergenic | 55.0% |
| !! | GTCGGCATGTTGGTGTTGGT+TGG | + | chr8.4:47616099-47616118 | MS.gene34231:intron | 55.0% |
| !! | GTTGGTGCCAGTGTTGTGTC+CGG | + | chr8.4:47616117-47616136 | MS.gene34231:intron | 55.0% |
| !! | TGGTGTCGGCATGTTGGTGT+TGG | + | chr8.4:47616095-47616114 | MS.gene34231:intron | 55.0% |
| AGAGAGCTCGTCAGGCTGAG+AGG | + | chr8.4:47615943-47615962 | MS.gene34231:CDS | 60.0% | |
| ! | CAGGGGCATACCAGCCATGA+TGG | - | chr8.4:47617199-47617218 | None:intergenic | 60.0% |
| ! | CCCTGGAGGCGCAAATTAGG+TGG | + | chr8.4:47616605-47616624 | MS.gene34231:intron | 60.0% |
| !! | CAGACACTGGCTTGAGGCAC+AGG | - | chr8.4:47617218-47617237 | None:intergenic | 60.0% |
| !! | GACACTGGCTTGAGGCACAG+GGG | - | chr8.4:47617216-47617235 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 47615771 | 47617238 | 47615771 | ID=MS.gene34231 |
| chr8.4 | mRNA | 47615771 | 47617238 | 47615771 | ID=MS.gene34231.t1;Parent=MS.gene34231 |
| chr8.4 | exon | 47615771 | 47616019 | 47615771 | ID=MS.gene34231.t1.exon1;Parent=MS.gene34231.t1 |
| chr8.4 | CDS | 47615771 | 47616019 | 47615771 | ID=cds.MS.gene34231.t1;Parent=MS.gene34231.t1 |
| chr8.4 | exon | 47617008 | 47617238 | 47617008 | ID=MS.gene34231.t1.exon2;Parent=MS.gene34231.t1 |
| chr8.4 | CDS | 47617008 | 47617238 | 47617008 | ID=cds.MS.gene34231.t1;Parent=MS.gene34231.t1 |
| Gene Sequence |
| Protein sequence |
