Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08664.t1 | XP_013455971.1 | 96.6 | 117 | 4 | 0 | 1 | 117 | 1 | 117 | 4.40E-52 | 214.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08664.t1 | A0A072UJY4 | 96.6 | 117 | 4 | 0 | 1 | 117 | 1 | 117 | 3.1e-52 | 214.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene08664 | 0.803223 | 3.86E-49 | -1.69E-46 |
| MS.gene049151 | MS.gene08664 | 0.804537 | 2.06E-49 | -1.69E-46 |
| MS.gene049284 | MS.gene08664 | 0.850512 | 1.62E-60 | -1.69E-46 |
| MS.gene049346 | MS.gene08664 | 0.845436 | 4.07E-59 | -1.69E-46 |
| MS.gene049351 | MS.gene08664 | 0.840889 | 6.62E-58 | -1.69E-46 |
| MS.gene049389 | MS.gene08664 | 0.809714 | 1.65E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene08664 | 0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene049491 | MS.gene08664 | 0.849943 | 2.34E-60 | -1.69E-46 |
| MS.gene049599 | MS.gene08664 | -0.84126 | 5.29E-58 | -1.69E-46 |
| MS.gene049661 | MS.gene08664 | 0.876034 | 1.92E-68 | -1.69E-46 |
| MS.gene049662 | MS.gene08664 | 0.880156 | 6.92E-70 | -1.69E-46 |
| MS.gene049758 | MS.gene08664 | 0.900746 | 5.38E-78 | -1.69E-46 |
| MS.gene049910 | MS.gene08664 | 0.846038 | 2.79E-59 | -1.69E-46 |
| MS.gene049915 | MS.gene08664 | 0.817422 | 3.32E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene08664 | 0.86849 | 6.28E-66 | -1.69E-46 |
| MS.gene050099 | MS.gene08664 | 0.832559 | 8.82E-56 | -1.69E-46 |
| MS.gene050128 | MS.gene08664 | 0.860413 | 2.10E-63 | -1.69E-46 |
| MS.gene050301 | MS.gene08664 | 0.896637 | 3.05E-76 | -1.69E-46 |
| MS.gene050397 | MS.gene08664 | 0.874921 | 4.63E-68 | -1.69E-46 |
| MS.gene050404 | MS.gene08664 | 0.862848 | 3.79E-64 | -1.69E-46 |
| MS.gene050406 | MS.gene08664 | 0.866234 | 3.31E-65 | -1.69E-46 |
| MS.gene050432 | MS.gene08664 | 0.842927 | 1.92E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene08664 | 0.859878 | 3.05E-63 | -1.69E-46 |
| MS.gene050529 | MS.gene08664 | 0.892816 | 1.12E-74 | -1.69E-46 |
| MS.gene050558 | MS.gene08664 | 0.804657 | 1.94E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene08664 | 0.828704 | 7.75E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene08664 | 0.830271 | 3.23E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene08664 | 0.80432 | 2.29E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene08664 | 0.80749 | 4.93E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene08664 | 0.809766 | 1.61E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene08664 | 0.812985 | 3.22E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene08664 | 0.875458 | 3.03E-68 | -1.69E-46 |
| MS.gene05105 | MS.gene08664 | 0.819459 | 1.15E-52 | -1.69E-46 |
| MS.gene05119 | MS.gene08664 | -0.817202 | 3.73E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene08664 | 0.887594 | 1.24E-72 | -1.69E-46 |
| MS.gene051582 | MS.gene08664 | 0.811165 | 8.02E-51 | -1.69E-46 |
| MS.gene051662 | MS.gene08664 | 0.833997 | 3.87E-56 | -1.69E-46 |
| MS.gene051663 | MS.gene08664 | 0.845437 | 4.06E-59 | -1.69E-46 |
| MS.gene051860 | MS.gene08664 | 0.862006 | 6.88E-64 | -1.69E-46 |
| MS.gene051877 | MS.gene08664 | 0.827484 | 1.53E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene08664 | 0.81837 | 2.03E-52 | -1.69E-46 |
| MS.gene052077 | MS.gene08664 | 0.867422 | 1.38E-65 | -1.69E-46 |
| MS.gene052078 | MS.gene08664 | 0.900474 | 7.07E-78 | -1.69E-46 |
| MS.gene052178 | MS.gene08664 | 0.881261 | 2.78E-70 | -1.69E-46 |
| MS.gene052188 | MS.gene08664 | 0.888307 | 6.63E-73 | -1.69E-46 |
| MS.gene052207 | MS.gene08664 | 0.808566 | 2.91E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene08664 | 0.868623 | 5.69E-66 | -1.69E-46 |
| MS.gene052290 | MS.gene08664 | 0.823297 | 1.49E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene08664 | 0.900819 | 5.00E-78 | -1.69E-46 |
| MS.gene052322 | MS.gene08664 | 0.809282 | 2.04E-50 | -1.69E-46 |
| MS.gene052326 | MS.gene08664 | 0.853052 | 3.08E-61 | -1.69E-46 |
| MS.gene052340 | MS.gene08664 | 0.889952 | 1.53E-73 | -1.69E-46 |
| MS.gene052481 | MS.gene08664 | 0.812451 | 4.21E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene08664 | 0.836706 | 8.00E-57 | -1.69E-46 |
| MS.gene052917 | MS.gene08664 | -0.806537 | 7.84E-50 | -1.69E-46 |
| MS.gene053073 | MS.gene08664 | 0.81489 | 1.22E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene08664 | 0.817476 | 3.23E-52 | -1.69E-46 |
| MS.gene05316 | MS.gene08664 | 0.810254 | 1.26E-50 | -1.69E-46 |
| MS.gene053181 | MS.gene08664 | 0.812779 | 3.57E-51 | -1.69E-46 |
| MS.gene053209 | MS.gene08664 | 0.848278 | 6.79E-60 | -1.69E-46 |
| MS.gene053557 | MS.gene08664 | 0.871035 | 9.29E-67 | -1.69E-46 |
| MS.gene053559 | MS.gene08664 | 0.813249 | 2.82E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene08664 | 0.907916 | 3.02E-81 | -1.69E-46 |
| MS.gene053907 | MS.gene08664 | 0.820322 | 7.29E-53 | -1.69E-46 |
| MS.gene053928 | MS.gene08664 | 0.811356 | 7.29E-51 | -1.69E-46 |
| MS.gene053971 | MS.gene08664 | 0.835264 | 1.86E-56 | -1.69E-46 |
| MS.gene054010 | MS.gene08664 | 0.805626 | 1.22E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene08664 | 0.842546 | 2.42E-58 | -1.69E-46 |
| MS.gene054012 | MS.gene08664 | 0.830362 | 3.06E-55 | -1.69E-46 |
| MS.gene054013 | MS.gene08664 | 0.826142 | 3.19E-54 | -1.69E-46 |
| MS.gene054123 | MS.gene08664 | 0.864821 | 9.23E-65 | -1.69E-46 |
| MS.gene054214 | MS.gene08664 | 0.809734 | 1.63E-50 | -1.69E-46 |
| MS.gene054349 | MS.gene08664 | 0.848449 | 6.09E-60 | -1.69E-46 |
| MS.gene054376 | MS.gene08664 | 0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene08664 | 0.84998 | 2.28E-60 | -1.69E-46 |
| MS.gene054542 | MS.gene08664 | 0.813675 | 2.27E-51 | -1.69E-46 |
| MS.gene05455 | MS.gene08664 | 0.832054 | 1.18E-55 | -1.69E-46 |
| MS.gene054802 | MS.gene08664 | 0.813441 | 2.56E-51 | -1.69E-46 |
| MS.gene054803 | MS.gene08664 | 0.814674 | 1.37E-51 | -1.69E-46 |
| MS.gene054804 | MS.gene08664 | 0.813943 | 1.98E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene08664 | 0.827643 | 1.40E-54 | -1.69E-46 |
| MS.gene054955 | MS.gene08664 | 0.849998 | 2.26E-60 | -1.69E-46 |
| MS.gene054983 | MS.gene08664 | 0.858934 | 5.86E-63 | -1.69E-46 |
| MS.gene055116 | MS.gene08664 | 0.87 | 2.03E-66 | -1.69E-46 |
| MS.gene055254 | MS.gene08664 | 0.822315 | 2.53E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene08664 | 0.89981 | 1.37E-77 | -1.69E-46 |
| MS.gene055390 | MS.gene08664 | 0.854978 | 8.59E-62 | -1.69E-46 |
| MS.gene055422 | MS.gene08664 | 0.90254 | 8.73E-79 | -1.69E-46 |
| MS.gene055428 | MS.gene08664 | 0.898702 | 4.09E-77 | -1.69E-46 |
| MS.gene055533 | MS.gene08664 | 0.823087 | 1.67E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene08664 | 0.821689 | 3.53E-53 | -1.69E-46 |
| MS.gene055604 | MS.gene08664 | 0.845794 | 3.25E-59 | -1.69E-46 |
| MS.gene055712 | MS.gene08664 | 0.819571 | 1.08E-52 | -1.69E-46 |
| MS.gene055896 | MS.gene08664 | 0.821044 | 4.98E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene08664 | 0.847161 | 1.38E-59 | -1.69E-46 |
| MS.gene056314 | MS.gene08664 | 0.848922 | 4.50E-60 | -1.69E-46 |
| MS.gene056407 | MS.gene08664 | 0.832593 | 8.65E-56 | -1.69E-46 |
| MS.gene056410 | MS.gene08664 | 0.824392 | 8.27E-54 | -1.69E-46 |
| MS.gene056451 | MS.gene08664 | 0.812496 | 4.12E-51 | -1.69E-46 |
| MS.gene056609 | MS.gene08664 | 0.862009 | 6.86E-64 | -1.69E-46 |
| MS.gene056612 | MS.gene08664 | 0.902319 | 1.09E-78 | -1.69E-46 |
| MS.gene056659 | MS.gene08664 | 0.824736 | 6.87E-54 | -1.69E-46 |
| MS.gene056719 | MS.gene08664 | 0.818469 | 1.93E-52 | -1.69E-46 |
| MS.gene056772 | MS.gene08664 | 0.832029 | 1.19E-55 | -1.69E-46 |
| MS.gene056796 | MS.gene08664 | 0.815609 | 8.47E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene08664 | -0.819471 | 1.14E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene08664 | 0.814444 | 1.54E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene08664 | 0.889296 | 2.75E-73 | -1.69E-46 |
| MS.gene057121 | MS.gene08664 | 0.85324 | 2.72E-61 | -1.69E-46 |
| MS.gene057123 | MS.gene08664 | 0.838283 | 3.15E-57 | -1.69E-46 |
| MS.gene057146 | MS.gene08664 | 0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene057245 | MS.gene08664 | 0.836852 | 7.34E-57 | -1.69E-46 |
| MS.gene057295 | MS.gene08664 | 0.83092 | 2.24E-55 | -1.69E-46 |
| MS.gene05733 | MS.gene08664 | 0.872808 | 2.39E-67 | -1.69E-46 |
| MS.gene057396 | MS.gene08664 | 0.813668 | 2.28E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene08664 | 0.849568 | 2.97E-60 | -1.69E-46 |
| MS.gene057448 | MS.gene08664 | 0.838442 | 2.87E-57 | -1.69E-46 |
| MS.gene057449 | MS.gene08664 | 0.824215 | 9.11E-54 | -1.69E-46 |
| MS.gene057450 | MS.gene08664 | 0.847673 | 9.97E-60 | -1.69E-46 |
| MS.gene057749 | MS.gene08664 | 0.828815 | 7.29E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene08664 | 0.810234 | 1.28E-50 | -1.69E-46 |
| MS.gene057779 | MS.gene08664 | 0.826933 | 2.07E-54 | -1.69E-46 |
| MS.gene057782 | MS.gene08664 | 0.811963 | 5.38E-51 | -1.69E-46 |
| MS.gene057794 | MS.gene08664 | 0.810319 | 1.22E-50 | -1.69E-46 |
| MS.gene057796 | MS.gene08664 | 0.838943 | 2.13E-57 | -1.69E-46 |
| MS.gene057823 | MS.gene08664 | 0.820246 | 7.59E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene08664 | 0.881757 | 1.84E-70 | -1.69E-46 |
| MS.gene058082 | MS.gene08664 | 0.855336 | 6.76E-62 | -1.69E-46 |
| MS.gene05811 | MS.gene08664 | 0.894639 | 2.04E-75 | -1.69E-46 |
| MS.gene058142 | MS.gene08664 | 0.848561 | 5.67E-60 | -1.69E-46 |
| MS.gene058233 | MS.gene08664 | 0.87066 | 1.23E-66 | -1.69E-46 |
| MS.gene058276 | MS.gene08664 | 0.830905 | 2.26E-55 | -1.69E-46 |
| MS.gene058293 | MS.gene08664 | 0.842916 | 1.93E-58 | -1.69E-46 |
| MS.gene058365 | MS.gene08664 | 0.845583 | 3.71E-59 | -1.69E-46 |
| MS.gene058660 | MS.gene08664 | 0.898982 | 3.11E-77 | -1.69E-46 |
| MS.gene058710 | MS.gene08664 | 0.827743 | 1.32E-54 | -1.69E-46 |
| MS.gene058749 | MS.gene08664 | 0.8456 | 3.67E-59 | -1.69E-46 |
| MS.gene058787 | MS.gene08664 | 0.836791 | 7.61E-57 | -1.69E-46 |
| MS.gene058845 | MS.gene08664 | 0.861871 | 7.57E-64 | -1.69E-46 |
| MS.gene05886 | MS.gene08664 | 0.804606 | 1.99E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene08664 | 0.869209 | 3.68E-66 | -1.69E-46 |
| MS.gene05907 | MS.gene08664 | 0.892328 | 1.76E-74 | -1.69E-46 |
| MS.gene059285 | MS.gene08664 | 0.863709 | 2.05E-64 | -1.69E-46 |
| MS.gene059294 | MS.gene08664 | 0.855249 | 7.16E-62 | -1.69E-46 |
| MS.gene059326 | MS.gene08664 | 0.830716 | 2.51E-55 | -1.69E-46 |
| MS.gene059342 | MS.gene08664 | 0.823617 | 1.26E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene08664 | 0.819657 | 1.04E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene08664 | 0.870269 | 1.66E-66 | -1.69E-46 |
| MS.gene059536 | MS.gene08664 | 0.804264 | 2.35E-49 | -1.69E-46 |
| MS.gene059597 | MS.gene08664 | 0.89965 | 1.61E-77 | -1.69E-46 |
| MS.gene05959 | MS.gene08664 | 0.825045 | 5.81E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene08664 | 0.856747 | 2.61E-62 | -1.69E-46 |
| MS.gene059688 | MS.gene08664 | 0.883315 | 4.98E-71 | -1.69E-46 |
| MS.gene059692 | MS.gene08664 | 0.89756 | 1.25E-76 | -1.69E-46 |
| MS.gene059695 | MS.gene08664 | 0.891284 | 4.57E-74 | -1.69E-46 |
| MS.gene059708 | MS.gene08664 | 0.836291 | 1.02E-56 | -1.69E-46 |
| MS.gene059807 | MS.gene08664 | 0.807025 | 6.18E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene08664 | 0.887428 | 1.44E-72 | -1.69E-46 |
| MS.gene059894 | MS.gene08664 | 0.915451 | 5.79E-85 | -1.69E-46 |
| MS.gene059899 | MS.gene08664 | 0.83039 | 3.02E-55 | -1.69E-46 |
| MS.gene059960 | MS.gene08664 | 0.807323 | 5.35E-50 | -1.69E-46 |
| MS.gene059974 | MS.gene08664 | 0.802057 | 6.72E-49 | -1.69E-46 |
| MS.gene060005 | MS.gene08664 | 0.811034 | 8.57E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene08664 | 0.881803 | 1.77E-70 | -1.69E-46 |
| MS.gene060403 | MS.gene08664 | 0.817553 | 3.11E-52 | -1.69E-46 |
| MS.gene060407 | MS.gene08664 | 0.845928 | 2.99E-59 | -1.69E-46 |
| MS.gene060476 | MS.gene08664 | 0.860236 | 2.38E-63 | -1.69E-46 |
| MS.gene060495 | MS.gene08664 | 0.823334 | 1.46E-53 | -1.69E-46 |
| MS.gene060804 | MS.gene08664 | 0.818995 | 1.47E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene08664 | 0.801983 | 6.96E-49 | -1.69E-46 |
| MS.gene06090 | MS.gene08664 | 0.823394 | 1.42E-53 | -1.69E-46 |
| MS.gene060986 | MS.gene08664 | 0.802668 | 5.03E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene08664 | 0.817045 | 4.04E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene08664 | 0.93455 | 3.40E-96 | -1.69E-46 |
| MS.gene061051 | MS.gene08664 | -0.851194 | 1.04E-60 | -1.69E-46 |
| MS.gene061112 | MS.gene08664 | 0.827815 | 1.27E-54 | -1.69E-46 |
| MS.gene061265 | MS.gene08664 | 0.831435 | 1.67E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08664.t1 | MTR_4g057845 | 96.581 | 117 | 4 | 0 | 1 | 117 | 1 | 117 | 1.44e-78 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08664.t1 | AT3G45050 | 59.633 | 109 | 42 | 2 | 10 | 116 | 22 | 130 | 4.41e-35 | 119 |
| MS.gene08664.t1 | AT3G45050 | 59.633 | 109 | 42 | 2 | 11 | 117 | 23 | 131 | 3.31e-34 | 117 |
| MS.gene08664.t1 | AT3G45050 | 59.633 | 109 | 42 | 2 | 11 | 117 | 23 | 131 | 3.31e-34 | 117 |
| MS.gene08664.t1 | AT3G45050 | 59.633 | 109 | 42 | 2 | 11 | 117 | 23 | 131 | 3.31e-34 | 117 |
Find 29 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACGTATCCTTCACCTTTGC+AGG | 0.266782 | 4.3:-43202731 | None:intergenic |
| GGTAAGCAGCGAAGTGATAT+TGG | 0.298796 | 4.3:-43200304 | None:intergenic |
| GTGCAGTAGCTCCACTTCTT+TGG | 0.304778 | 4.3:-43202919 | None:intergenic |
| CTGTGTGGACTCAGAAGTTT+TGG | 0.321230 | 4.3:-43201806 | None:intergenic |
| TGCGAAGCTTGTGGAGGTTC+TGG | 0.323223 | 4.3:+43202697 | MS.gene08664:CDS |
| TACTGCACAAAATGCTCTTC+TGG | 0.383793 | 4.3:+43202934 | MS.gene08664:CDS |
| GTAAGCAGCGAAGTGATATT+GGG | 0.423565 | 4.3:-43200303 | None:intergenic |
| GCTCCACTTCTTTGGAAGCC+TGG | 0.444307 | 4.3:-43202911 | None:intergenic |
| TATCCTTCACCTTTGCAGGC+TGG | 0.492824 | 4.3:-43202727 | None:intergenic |
| AAACATGGCAACTCGATTCA+CGG | 0.496179 | 4.3:+43202804 | MS.gene08664:CDS |
| GCCACGGAAACTGCAGTCTC+AGG | 0.499889 | 4.3:-43201733 | None:intergenic |
| TGTTCAACATGTGGTGGAAG+TGG | 0.503858 | 4.3:+43202964 | MS.gene08664:CDS |
| TGAAGTCACAATTGCAAACA+AGG | 0.519947 | 4.3:+43201683 | MS.gene08664:CDS |
| TTCTGAAGAAAGTGCTGAGA+AGG | 0.545376 | 4.3:+43202765 | MS.gene08664:CDS |
| TTCAAAGAATGCGAAGCTTG+TGG | 0.553196 | 4.3:+43202688 | MS.gene08664:CDS |
| TGGCAACTCGATTCACGGAT+GGG | 0.558434 | 4.3:+43202809 | MS.gene08664:CDS |
| GCCTGAGACTGCAGTTTCCG+TGG | 0.567805 | 4.3:+43201732 | MS.gene08664:CDS |
| TGTCCAGCCTGCAAAGGTGA+AGG | 0.582605 | 4.3:+43202724 | MS.gene08664:CDS |
| ACACCAGGCTTCCAAAGAAG+TGG | 0.583227 | 4.3:+43202908 | MS.gene08664:CDS |
| GGTTCTCTGTACAAGAAGAG+TGG | 0.585572 | 4.3:-43201785 | None:intergenic |
| ATGGCAACTCGATTCACGGA+TGG | 0.587327 | 4.3:+43202808 | MS.gene08664:CDS |
| AAAAGTTGCTGCTACTGCCA+CGG | 0.603898 | 4.3:-43201749 | None:intergenic |
| ACTGAATTTCTACATACACC+AGG | 0.610431 | 4.3:+43202893 | MS.gene08664:CDS |
| GGTATATGTCCAGCCTGCAA+AGG | 0.614856 | 4.3:+43202718 | MS.gene08664:CDS |
| AAAGAATGCGAAGCTTGTGG+AGG | 0.643600 | 4.3:+43202691 | MS.gene08664:CDS |
| GGCAGAAACTGTTCAACATG+TGG | 0.690362 | 4.3:+43202955 | MS.gene08664:CDS |
| AGAAACTGTTCAACATGTGG+TGG | 0.691039 | 4.3:+43202958 | MS.gene08664:CDS |
| CAAAACTTCTGAGTCCACAC+AGG | 0.698030 | 4.3:+43201807 | MS.gene08664:CDS |
| AGATGGGTTACTGACCTGTG+TGG | 0.759513 | 4.3:-43201821 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCAAAATTAAAAATTTTAG+AGG | - | chr4.3:43202862-43202881 | None:intergenic | 10.0% |
| !!! | AATTAAAAATTTTAGAGGAA+AGG | - | chr4.3:43202857-43202876 | None:intergenic | 15.0% |
| !!! | TAATTATGGTAACTTAATTT+TGG | - | chr4.3:43200613-43200632 | None:intergenic | 15.0% |
| !!! | TCATTTTCTCAAATTATTAT+TGG | + | chr4.3:43201961-43201980 | MS.gene08664:intron | 15.0% |
| !! | AAGAAAACTATCTAAAAACA+TGG | + | chr4.3:43202789-43202808 | MS.gene08664:CDS | 20.0% |
| !! | ATAGTTTCACATGAAAAATT+TGG | - | chr4.3:43202657-43202676 | None:intergenic | 20.0% |
| !! | CAATTCAATCATATTATGAA+TGG | - | chr4.3:43202630-43202649 | None:intergenic | 20.0% |
| !! | TACTTCATAATTTAAACTGA+GGG | - | chr4.3:43201519-43201538 | None:intergenic | 20.0% |
| !! | TCTCAAATTATTAATGATGT+CGG | + | chr4.3:43202152-43202171 | MS.gene08664:intron | 20.0% |
| !! | TTACTTCATAATTTAAACTG+AGG | - | chr4.3:43201520-43201539 | None:intergenic | 20.0% |
| !! | TTGATGTTGTAAAATGAAAA+GGG | + | chr4.3:43201617-43201636 | MS.gene08664:intron | 20.0% |
| !!! | AAAAGATTTTGTAGAATTTG+AGG | + | chr4.3:43201400-43201419 | MS.gene08664:intron | 20.0% |
| !!! | AAGATTTTTATGTTCTTGTT+TGG | - | chr4.3:43202248-43202267 | None:intergenic | 20.0% |
| !!! | ACTATGATGATTTTTAACAT+AGG | - | chr4.3:43200515-43200534 | None:intergenic | 20.0% |
| !!! | AGTTTTAGGTACTAAATAAT+TGG | + | chr4.3:43200809-43200828 | MS.gene08664:intron | 20.0% |
| !!! | TTTTTAACATAGGAATGAAT+TGG | - | chr4.3:43200505-43200524 | None:intergenic | 20.0% |
| ! | AAAACTAACTACTAAGCTTT+AGG | - | chr4.3:43200417-43200436 | None:intergenic | 25.0% |
| ! | ATGATTTATGACTAGACATT+TGG | - | chr4.3:43201014-43201033 | None:intergenic | 25.0% |
| ! | ATTGATACTAACAATAGAGA+TGG | - | chr4.3:43201841-43201860 | None:intergenic | 25.0% |
| ! | CTACAGTAAAACTTAATCAT+TGG | + | chr4.3:43201152-43201171 | MS.gene08664:intron | 25.0% |
| ! | CTTGATGTTGTAAAATGAAA+AGG | + | chr4.3:43201616-43201635 | MS.gene08664:intron | 25.0% |
| ! | GCTTCTTCAAAAAAAATCAT+TGG | + | chr4.3:43201287-43201306 | MS.gene08664:intron | 25.0% |
| ! | TAAATTGAAATCTCACTTTG+AGG | - | chr4.3:43200328-43200347 | None:intergenic | 25.0% |
| ! | TATGTTTATGACTAGACATT+TGG | - | chr4.3:43201062-43201081 | None:intergenic | 25.0% |
| ! | TTATCAAACATTTGAACTAG+AGG | - | chr4.3:43202281-43202300 | None:intergenic | 25.0% |
| ! | TTCAATGTAATCATTTGTGT+CGG | - | chr4.3:43201936-43201955 | None:intergenic | 25.0% |
| ! | TTGATACTAACAATAGAGAT+GGG | - | chr4.3:43201840-43201859 | None:intergenic | 25.0% |
| !! | ACCATCTTAAAATTTGAGTT+TGG | - | chr4.3:43200933-43200952 | None:intergenic | 25.0% |
| !! | CAATGATTAAGTTTTACTGT+AGG | - | chr4.3:43201154-43201173 | None:intergenic | 25.0% |
| !! | CTTTGATGATAAGTTTCAAA+AGG | + | chr4.3:43201432-43201451 | MS.gene08664:intron | 25.0% |
| !! | TCATTAATTTTCAGAACAGT+AGG | + | chr4.3:43200556-43200575 | MS.gene08664:intron | 25.0% |
| !!! | AAAATTTTAGAGGAAAGGAA+AGG | - | chr4.3:43202852-43202871 | None:intergenic | 25.0% |
| !!! | ACTGTTCTGAAAATTAATGA+AGG | - | chr4.3:43200556-43200575 | None:intergenic | 25.0% |
| !!! | ATGTGGTTTTTACTTTTTAC+TGG | + | chr4.3:43200449-43200468 | MS.gene08664:intron | 25.0% |
| !!! | TAAAGCTTAGTAGTTAGTTT+TGG | + | chr4.3:43200416-43200435 | MS.gene08664:intron | 25.0% |
| !!! | TCCAAACTCAAATTTTAAGA+TGG | + | chr4.3:43200929-43200948 | MS.gene08664:intron | 25.0% |
| !!! | TGTGGTTTTTACTTTTTACT+GGG | + | chr4.3:43200450-43200469 | MS.gene08664:intron | 25.0% |
| !!! | TTTCTTTTCAAGTTTGTGTT+TGG | + | chr4.3:43200725-43200744 | MS.gene08664:intron | 25.0% |
| AAAATCATCATAGTCACGTT+AGG | + | chr4.3:43200521-43200540 | MS.gene08664:intron | 30.0% | |
| AATTAATGCTGCAGGAAAAT+AGG | + | chr4.3:43200628-43200647 | MS.gene08664:intron | 30.0% | |
| ACAGACACGAATATAAATCA+TGG | - | chr4.3:43202194-43202213 | None:intergenic | 30.0% | |
| GTCTTAATTTAACTCTTGTG+TGG | + | chr4.3:43200662-43200681 | MS.gene08664:intron | 30.0% | |
| GTGTTTGGATTAAATGAGTT+TGG | + | chr4.3:43200740-43200759 | MS.gene08664:intron | 30.0% | |
| TATGCTAATACTAGTAAAGC+TGG | - | chr4.3:43201111-43201130 | None:intergenic | 30.0% | |
| TCTATCTCTAGCATAGAAAA+TGG | + | chr4.3:43201334-43201353 | MS.gene08664:intron | 30.0% | |
| TTAGTACCTAAAACTACTGA+AGG | - | chr4.3:43200804-43200823 | None:intergenic | 30.0% | |
| TTGGATTAAATGAGTTTGGT+AGG | + | chr4.3:43200744-43200763 | MS.gene08664:intron | 30.0% | |
| ! | AATTTTCAGAACAGTAGGAT+TGG | + | chr4.3:43200561-43200580 | MS.gene08664:intron | 30.0% |
| ! | TACAATACAAGAAGCATGAT+TGG | - | chr4.3:43200358-43200377 | None:intergenic | 30.0% |
| ! | TGTTCTTGTTTGGAAAAGTA+AGG | - | chr4.3:43202238-43202257 | None:intergenic | 30.0% |
| ! | TTTTCCTGCAGCATTAATTA+TGG | - | chr4.3:43200627-43200646 | None:intergenic | 30.0% |
| !! | CCTGAAAATGTGTTTTAAGT+GGG | + | chr4.3:43200901-43200920 | MS.gene08664:intron | 30.0% |
| AACATAGGAATGAATTGGCA+CGG | - | chr4.3:43200500-43200519 | None:intergenic | 35.0% | |
| ACTCTTGTGTGGTTTCTATA+TGG | + | chr4.3:43200673-43200692 | MS.gene08664:intron | 35.0% | |
| ACTGAATTTCTACATACACC+AGG | + | chr4.3:43202893-43202912 | MS.gene08664:CDS | 35.0% | |
| ATCAGTGGATCATTTGCTTA+GGG | - | chr4.3:43201580-43201599 | None:intergenic | 35.0% | |
| CAACTTCATGTGAGAATAGT+CGG | - | chr4.3:43201546-43201565 | None:intergenic | 35.0% | |
| CAAGATAAGTATCCAATACC+AGG | - | chr4.3:43201906-43201925 | None:intergenic | 35.0% | |
| GATCCACTGATAAGAACTTA+TGG | + | chr4.3:43201589-43201608 | MS.gene08664:intron | 35.0% | |
| GTTACCATAATTAATGCTGC+AGG | + | chr4.3:43200620-43200639 | MS.gene08664:intron | 35.0% | |
| TAGCCATAAGTTCTTATCAG+TGG | - | chr4.3:43201595-43201614 | None:intergenic | 35.0% | |
| TATAATGTAACCATGTGTGC+TGG | - | chr4.3:43202121-43202140 | None:intergenic | 35.0% | |
| TATCAGTGGATCATTTGCTT+AGG | - | chr4.3:43201581-43201600 | None:intergenic | 35.0% | |
| TATTACCTGCATCTACATGT+TGG | + | chr4.3:43202369-43202388 | MS.gene08664:intron | 35.0% | |
| TGAAGTCACAATTGCAAACA+AGG | + | chr4.3:43201683-43201702 | MS.gene08664:CDS | 35.0% | |
| TGTCTCTCTTCTACATAAGT+GGG | - | chr4.3:43202066-43202085 | None:intergenic | 35.0% | |
| ! | CCCACTTAAAACACATTTTC+AGG | - | chr4.3:43200904-43200923 | None:intergenic | 35.0% |
| ! | GTGATTCCTTCAGTAGTTTT+AGG | + | chr4.3:43200795-43200814 | MS.gene08664:intron | 35.0% |
| ! | TACAAGAAGCATGATTGGTT+AGG | - | chr4.3:43200353-43200372 | None:intergenic | 35.0% |
| ! | TCTTGTTTGGAAAAGTAAGG+CGG | - | chr4.3:43202235-43202254 | None:intergenic | 35.0% |
| ! | TTGGGAGTTGACTTAATTGT+TGG | + | chr4.3:43200871-43200890 | MS.gene08664:intron | 35.0% |
| !! | GAAAATGTGTTTTAAGTGGG+AGG | + | chr4.3:43200904-43200923 | MS.gene08664:intron | 35.0% |
| !! | GCCTGAAAATGTGTTTTAAG+TGG | + | chr4.3:43200900-43200919 | MS.gene08664:intron | 35.0% |
| AAACATGGCAACTCGATTCA+CGG | + | chr4.3:43202804-43202823 | MS.gene08664:CDS | 40.0% | |
| AGAAACTGTTCAACATGTGG+TGG | + | chr4.3:43202958-43202977 | MS.gene08664:CDS | 40.0% | |
| AGCACAAACTAGCGACATAT+AGG | + | chr4.3:43202468-43202487 | MS.gene08664:intron | 40.0% | |
| ATCCACATAGACACAAACAC+AGG | - | chr4.3:43202403-43202422 | None:intergenic | 40.0% | |
| CAAATGCGGTTTGTGTACTT+GGG | + | chr4.3:43200853-43200872 | MS.gene08664:intron | 40.0% | |
| CTTGTGTGGTTTCTATATGG+TGG | + | chr4.3:43200676-43200695 | MS.gene08664:intron | 40.0% | |
| GATTAGGTATGTCCTGGTAT+TGG | + | chr4.3:43201891-43201910 | MS.gene08664:intron | 40.0% | |
| GTAAGCAGCGAAGTGATATT+GGG | - | chr4.3:43200306-43200325 | None:intergenic | 40.0% | |
| GTGTCTCTCTTCTACATAAG+TGG | - | chr4.3:43202067-43202086 | None:intergenic | 40.0% | |
| TACACAAACCGCATTTGAGA+TGG | - | chr4.3:43200850-43200869 | None:intergenic | 40.0% | |
| TACTGCACAAAATGCTCTTC+TGG | + | chr4.3:43202934-43202953 | MS.gene08664:CDS | 40.0% | |
| TACTGGGCTGTTCTTTAGTT+TGG | + | chr4.3:43200466-43200485 | MS.gene08664:intron | 40.0% | |
| TATGAAGCACAGATACTCCT+CGG | + | chr4.3:43201869-43201888 | MS.gene08664:intron | 40.0% | |
| TCAAATGCGGTTTGTGTACT+TGG | + | chr4.3:43200852-43200871 | MS.gene08664:intron | 40.0% | |
| TGGAGTTCTTAAACCCACTT+GGG | + | chr4.3:43201172-43201191 | MS.gene08664:intron | 40.0% | |
| TTCAAAGAATGCGAAGCTTG+TGG | + | chr4.3:43202688-43202707 | MS.gene08664:CDS | 40.0% | |
| TTGGAGTTCTTAAACCCACT+TGG | + | chr4.3:43201171-43201190 | MS.gene08664:intron | 40.0% | |
| ! | AGTAGCAGCAACTTTTGTTG+GGG | + | chr4.3:43201756-43201775 | MS.gene08664:CDS | 40.0% |
| ! | CAGTAGCAGCAACTTTTGTT+GGG | + | chr4.3:43201755-43201774 | MS.gene08664:CDS | 40.0% |
| ! | GTAATCATTTGTGTCGGTGT+CGG | - | chr4.3:43201930-43201949 | None:intergenic | 40.0% |
| ! | GTCAATTTAGGTGGCTAGTT+TGG | + | chr4.3:43201265-43201284 | MS.gene08664:intron | 40.0% |
| !! | TGCTGTTTCCATCTCAAATG+CGG | + | chr4.3:43200839-43200858 | MS.gene08664:intron | 40.0% |
| !! | TTCTGAAGAAAGTGCTGAGA+AGG | + | chr4.3:43202765-43202784 | MS.gene08664:CDS | 40.0% |
| AAAAGTTGCTGCTACTGCCA+CGG | - | chr4.3:43201752-43201771 | None:intergenic | 45.0% | |
| AAAGAATGCGAAGCTTGTGG+AGG | + | chr4.3:43202691-43202710 | MS.gene08664:CDS | 45.0% | |
| ATGAAGCACAGTCTCAGACA+CGG | - | chr4.3:43201998-43202017 | None:intergenic | 45.0% | |
| CAAAACTTCTGAGTCCACAC+AGG | + | chr4.3:43201807-43201826 | MS.gene08664:CDS | 45.0% | |
| CTAGCCACCTAAATTGACAC+CGG | - | chr4.3:43201263-43201282 | None:intergenic | 45.0% | |
| GGCAGAAACTGTTCAACATG+TGG | + | chr4.3:43202955-43202974 | MS.gene08664:CDS | 45.0% | |
| GGTAAGCAGCGAAGTGATAT+TGG | - | chr4.3:43200307-43200326 | None:intergenic | 45.0% | |
| GGTTCTCTGTACAAGAAGAG+TGG | - | chr4.3:43201788-43201807 | None:intergenic | 45.0% | |
| GTCCTGTGTTTGTGTCTATG+TGG | + | chr4.3:43202398-43202417 | MS.gene08664:intron | 45.0% | |
| GTTCTTAAACCCACTTGGGT+TGG | + | chr4.3:43201176-43201195 | MS.gene08664:intron | 45.0% | |
| TGACACCAACATGTAGATGC+AGG | - | chr4.3:43202377-43202396 | None:intergenic | 45.0% | |
| TGGTTCAACGGAACTTGGAT+AGG | - | chr4.3:43200963-43200982 | None:intergenic | 45.0% | |
| ! | CGATTCTCCGGTGTCAATTT+AGG | + | chr4.3:43201253-43201272 | MS.gene08664:intron | 45.0% |
| ! | CTGTGTGGACTCAGAAGTTT+TGG | - | chr4.3:43201809-43201828 | None:intergenic | 45.0% |
| ! | GCAGTAGCAGCAACTTTTGT+TGG | + | chr4.3:43201754-43201773 | MS.gene08664:CDS | 45.0% |
| ! | TGTCTGAGACTGTGCTTCAT+AGG | + | chr4.3:43201998-43202017 | MS.gene08664:intron | 45.0% |
| ! | TGTTCAACATGTGGTGGAAG+TGG | + | chr4.3:43202964-43202983 | MS.gene08664:CDS | 45.0% |
| ! | TTCTCCGGTGTCAATTTAGG+TGG | + | chr4.3:43201256-43201275 | MS.gene08664:intron | 45.0% |
| !! | GTTTTGGTGTGCATGCAATG+TGG | + | chr4.3:43200432-43200451 | MS.gene08664:intron | 45.0% |
| !! | TGGGCTGTTCTTTAGTTTGG+TGG | + | chr4.3:43200469-43200488 | MS.gene08664:intron | 45.0% |
| !! | TGTAACCATGTGTGCTGGTT+TGG | - | chr4.3:43202116-43202135 | None:intergenic | 45.0% |
| ACACCAGGCTTCCAAAGAAG+TGG | + | chr4.3:43202908-43202927 | MS.gene08664:CDS | 50.0% | |
| ACAGGTGGTTCAACGGAACT+TGG | - | chr4.3:43200968-43200987 | None:intergenic | 50.0% | |
| ACCAGGACATACCTAATCCG+AGG | - | chr4.3:43201889-43201908 | None:intergenic | 50.0% | |
| ACCATCAGACCAACCCAAGT+GGG | - | chr4.3:43201188-43201207 | None:intergenic | 50.0% | |
| AGGTCTCAGGTTCGATTCTC+CGG | + | chr4.3:43201241-43201260 | MS.gene08664:intron | 50.0% | |
| ATCGAACCTGAGACCTTGAG+AGG | - | chr4.3:43201237-43201256 | None:intergenic | 50.0% | |
| ATGGCAACTCGATTCACGGA+TGG | + | chr4.3:43202808-43202827 | MS.gene08664:CDS | 50.0% | |
| CACGTATCCTTCACCTTTGC+AGG | - | chr4.3:43202734-43202753 | None:intergenic | 50.0% | |
| CTCGACAACAGGTGGTTCAA+CGG | - | chr4.3:43200975-43200994 | None:intergenic | 50.0% | |
| CTGCTACACGTGACACAACA+CGG | + | chr4.3:43202523-43202542 | MS.gene08664:intron | 50.0% | |
| GCACAGATACTCCTCGGATT+AGG | + | chr4.3:43201875-43201894 | MS.gene08664:intron | 50.0% | |
| GGTATATGTCCAGCCTGCAA+AGG | + | chr4.3:43202718-43202737 | MS.gene08664:CDS | 50.0% | |
| GTGCAGTAGCTCCACTTCTT+TGG | - | chr4.3:43202922-43202941 | None:intergenic | 50.0% | |
| GTTGTGTCACGTGTAGCAGT+TGG | - | chr4.3:43202522-43202541 | None:intergenic | 50.0% | |
| TATCCTTCACCTTTGCAGGC+TGG | - | chr4.3:43202730-43202749 | None:intergenic | 50.0% | |
| TGGCAACTCGATTCACGGAT+GGG | + | chr4.3:43202809-43202828 | MS.gene08664:CDS | 50.0% | |
| ! | TCCTCGGATTAGGTATGTCC+TGG | + | chr4.3:43201885-43201904 | MS.gene08664:intron | 50.0% |
| !! | AGATGGGTTACTGACCTGTG+TGG | - | chr4.3:43201824-43201843 | None:intergenic | 50.0% |
| !! | TGGTCTGATGGTGTTGGCTT+GGG | + | chr4.3:43201196-43201215 | MS.gene08664:intron | 50.0% |
| !! | TTGGGTTGGTCTGATGGTGT+TGG | + | chr4.3:43201190-43201209 | MS.gene08664:intron | 50.0% |
| !! | TTGGTCTGATGGTGTTGGCT+TGG | + | chr4.3:43201195-43201214 | MS.gene08664:intron | 50.0% |
| CACCATCAGACCAACCCAAG+TGG | - | chr4.3:43201189-43201208 | None:intergenic | 55.0% | |
| CACCTGTTGTCGAGTCACTC+GGG | + | chr4.3:43200981-43201000 | MS.gene08664:intron | 55.0% | |
| CCACCTGTTGTCGAGTCACT+CGG | + | chr4.3:43200980-43200999 | MS.gene08664:intron | 55.0% | |
| CTTGGAGTGTGCTCCTCTCA+AGG | + | chr4.3:43201221-43201240 | MS.gene08664:intron | 55.0% | |
| GTGTGCCAAACCAGCACACA+TGG | + | chr4.3:43202108-43202127 | MS.gene08664:intron | 55.0% | |
| TGCGAAGCTTGTGGAGGTTC+TGG | + | chr4.3:43202697-43202716 | MS.gene08664:CDS | 55.0% | |
| TGTCCAGCCTGCAAAGGTGA+AGG | + | chr4.3:43202724-43202743 | MS.gene08664:CDS | 55.0% | |
| TGTGCTCCTCTCAAGGTCTC+AGG | + | chr4.3:43201228-43201247 | MS.gene08664:intron | 55.0% | |
| ! | CTTGAGAGGAGCACACTCCA+AGG | - | chr4.3:43201223-43201242 | None:intergenic | 55.0% |
| ! | GCTCCACTTCTTTGGAAGCC+TGG | - | chr4.3:43202914-43202933 | None:intergenic | 55.0% |
| !! | ACCCACTTGGGTTGGTCTGA+TGG | + | chr4.3:43201184-43201203 | MS.gene08664:intron | 55.0% |
| !! | ATGGTGTTGGCTTGGGACCT+TGG | + | chr4.3:43201203-43201222 | MS.gene08664:intron | 55.0% |
| CCGAGTGACTCGACAACAGG+TGG | - | chr4.3:43200983-43201002 | None:intergenic | 60.0% | |
| GACCCGAGTGACTCGACAAC+AGG | - | chr4.3:43200986-43201005 | None:intergenic | 60.0% | |
| GCCACGGAAACTGCAGTCTC+AGG | - | chr4.3:43201736-43201755 | None:intergenic | 60.0% | |
| GCCTGAGACTGCAGTTTCCG+TGG | + | chr4.3:43201732-43201751 | MS.gene08664:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 43200283 | 43202998 | 43200283 | ID=MS.gene08664 |
| chr4.3 | mRNA | 43200283 | 43202998 | 43200283 | ID=MS.gene08664.t1;Parent=MS.gene08664 |
| chr4.3 | exon | 43200283 | 43200331 | 43200283 | ID=MS.gene08664.t1.exon1;Parent=MS.gene08664.t1 |
| chr4.3 | CDS | 43200283 | 43200331 | 43200283 | ID=cds.MS.gene08664.t1;Parent=MS.gene08664.t1 |
| chr4.3 | exon | 43201680 | 43201828 | 43201680 | ID=MS.gene08664.t1.exon2;Parent=MS.gene08664.t1 |
| chr4.3 | CDS | 43201680 | 43201828 | 43201680 | ID=cds.MS.gene08664.t1;Parent=MS.gene08664.t1 |
| chr4.3 | exon | 43202682 | 43202830 | 43202682 | ID=MS.gene08664.t1.exon3;Parent=MS.gene08664.t1 |
| chr4.3 | CDS | 43202682 | 43202830 | 43202682 | ID=cds.MS.gene08664.t1;Parent=MS.gene08664.t1 |
| chr4.3 | exon | 43202887 | 43202998 | 43202887 | ID=MS.gene08664.t1.exon4;Parent=MS.gene08664.t1 |
| chr4.3 | CDS | 43202887 | 43202998 | 43202887 | ID=cds.MS.gene08664.t1;Parent=MS.gene08664.t1 |
| Gene Sequence |
| Protein sequence |
