AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69174


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69174.t1 MTR_4g037505 97.222 108 2 1 1 107 1 108 5.56e-71 207
MS.gene69174.t1 MTR_3g030850 69.159 107 30 3 1 106 1 105 2.39e-41 132
MS.gene69174.t1 MTR_3g030860 68.519 108 30 4 1 107 1 105 3.22e-33 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69174.t1 AT1G51400 49.541 109 48 3 1 106 1 105 1.46e-23 87.4
MS.gene69174.t1 AT3G21055 50.909 110 44 4 1 107 1 103 1.31e-22 84.7
MS.gene69174.t1 AT3G21055 50.909 110 44 4 1 107 41 143 2.43e-22 85.1

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTTGCTGGAATGGCTT+TGG 0.303558 4.2:+65970423 MS.gene69174:CDS
GCAGCTGTTTGTTCTGTTGC+TGG 0.330654 4.2:+65970412 MS.gene69174:CDS
GCTTCAGGTGAACCACGTTT+TGG 0.335997 4.2:-65970454 None:intergenic
TTCAACTGCTTTGCCAGCAT+TGG 0.361259 4.2:-65970308 None:intergenic
GCATAGGCTTTCTTAGCTTC+AGG 0.364633 4.2:-65970469 None:intergenic
TGTTTGTTCTGTTGCTGGAA+TGG 0.390336 4.2:+65970417 MS.gene69174:CDS
GGTGGCAACTCAGAGGAAAC+TGG 0.431564 4.2:+65970279 MS.gene69174:CDS
GTTGGCATGGTGACACAAAC+AGG 0.454036 4.2:-65970493 None:intergenic
GAAGCTCAGCCATCGCGAAC+CGG 0.486000 4.2:+65970251 MS.gene69174:CDS
AGCCATCGCGAACCGGTCTC+CGG 0.486104 4.2:+65970258 MS.gene69174:CDS
TCTGAGTTGCCACCGGAGAC+CGG 0.487218 4.2:-65970270 None:intergenic
ACCATGACAGCAGCATTCTT+CGG 0.487676 4.2:+65970226 MS.gene69174:CDS
AAACTGGTGGTGGCCAATGC+TGG 0.497236 4.2:+65970295 MS.gene69174:CDS
CATGATGTTCCTCCTTCCAT+TGG 0.502517 4.2:-65970374 None:intergenic
GGTGAGTTATGACAATGAAA+AGG 0.503070 4.2:+65970345 MS.gene69174:CDS
TAGCGACAAATTCTAGCTGT+TGG 0.507947 4.2:-65970511 None:intergenic
AATGCTGGCAAAGCAGTTGA+AGG 0.545817 4.2:+65970310 MS.gene69174:CDS
CACCGGAGACCGGTTCGCGA+TGG 0.568043 4.2:-65970260 None:intergenic
GCCGAAGAATGCTGCTGTCA+TGG 0.580952 4.2:-65970227 None:intergenic
AGTTTCCTCTGAGTTGCCAC+CGG 0.580967 4.2:-65970277 None:intergenic
AACTCAGAGGAAACTGGTGG+TGG 0.588596 4.2:+65970285 MS.gene69174:CDS
GGTGACACAAACAGGAGCAT+AGG 0.599674 4.2:-65970485 None:intergenic
AATGAAAAGGAAGAGACCAA+TGG 0.609364 4.2:+65970358 MS.gene69174:CDS
AGTTGAAGGAGGGAAAATGA+AGG 0.612928 4.2:+65970324 MS.gene69174:CDS
CATCGCGAACCGGTCTCCGG+TGG 0.615926 4.2:+65970261 MS.gene69174:CDS
ACAAATTCTAGCTGTTGGCA+TGG 0.617400 4.2:-65970506 None:intergenic
GCTGGCAAAGCAGTTGAAGG+AGG 0.624856 4.2:+65970313 MS.gene69174:CDS
CTGGCAAAGCAGTTGAAGGA+GGG 0.628956 4.2:+65970314 MS.gene69174:CDS
AGGAAGAGACCAATGGAAGG+AGG 0.639763 4.2:+65970365 MS.gene69174:CDS
AAAAGGAAGAGACCAATGGA+AGG 0.647549 4.2:+65970362 MS.gene69174:CDS
TTGGCTGATGAGCCAAAACG+TGG 0.652108 4.2:+65970442 MS.gene69174:CDS
GGTCTCCGGTGGCAACTCAG+AGG 0.684571 4.2:+65970272 MS.gene69174:CDS
ATGACAGCAGCATTCTTCGG+CGG 0.688662 4.2:+65970229 MS.gene69174:CDS
GGCAACTCAGAGGAAACTGG+TGG 0.709130 4.2:+65970282 MS.gene69174:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATGAAAAGGAAGAGACCAA+TGG + chr4.2:65970358-65970377 MS.gene69174:CDS 35.0%
GGTGAGTTATGACAATGAAA+AGG + chr4.2:65970345-65970364 MS.gene69174:CDS 35.0%
AAAAGGAAGAGACCAATGGA+AGG + chr4.2:65970362-65970381 MS.gene69174:CDS 40.0%
AGTTGAAGGAGGGAAAATGA+AGG + chr4.2:65970324-65970343 MS.gene69174:CDS 40.0%
TGTTTGTTCTGTTGCTGGAA+TGG + chr4.2:65970417-65970436 MS.gene69174:CDS 40.0%
! ACAAATTCTAGCTGTTGGCA+TGG - chr4.2:65970509-65970528 None:intergenic 40.0%
! TAGCGACAAATTCTAGCTGT+TGG - chr4.2:65970514-65970533 None:intergenic 40.0%
AATGCTGGCAAAGCAGTTGA+AGG + chr4.2:65970310-65970329 MS.gene69174:CDS 45.0%
ACCATGACAGCAGCATTCTT+CGG + chr4.2:65970226-65970245 MS.gene69174:CDS 45.0%
CATGATGTTCCTCCTTCCAT+TGG - chr4.2:65970377-65970396 None:intergenic 45.0%
GCATAGGCTTTCTTAGCTTC+AGG - chr4.2:65970472-65970491 None:intergenic 45.0%
TTCAACTGCTTTGCCAGCAT+TGG - chr4.2:65970311-65970330 None:intergenic 45.0%
TTCTGTTGCTGGAATGGCTT+TGG + chr4.2:65970423-65970442 MS.gene69174:CDS 45.0%
AACTCAGAGGAAACTGGTGG+TGG + chr4.2:65970285-65970304 MS.gene69174:CDS 50.0%
AGGAAGAGACCAATGGAAGG+AGG + chr4.2:65970365-65970384 MS.gene69174:CDS 50.0%
AGTTTCCTCTGAGTTGCCAC+CGG - chr4.2:65970280-65970299 None:intergenic 50.0%
ATGACAGCAGCATTCTTCGG+CGG + chr4.2:65970229-65970248 MS.gene69174:CDS 50.0%
CTGGCAAAGCAGTTGAAGGA+GGG + chr4.2:65970314-65970333 MS.gene69174:CDS 50.0%
GCAGCTGTTTGTTCTGTTGC+TGG + chr4.2:65970412-65970431 MS.gene69174:CDS 50.0%
GTTGGCATGGTGACACAAAC+AGG - chr4.2:65970496-65970515 None:intergenic 50.0%
TTGGCTGATGAGCCAAAACG+TGG + chr4.2:65970442-65970461 MS.gene69174:CDS 50.0%
! GCTTCAGGTGAACCACGTTT+TGG - chr4.2:65970457-65970476 None:intergenic 50.0%
! GGTGACACAAACAGGAGCAT+AGG - chr4.2:65970488-65970507 None:intergenic 50.0%
GCCGAAGAATGCTGCTGTCA+TGG - chr4.2:65970230-65970249 None:intergenic 55.0%
GCTGGCAAAGCAGTTGAAGG+AGG + chr4.2:65970313-65970332 MS.gene69174:CDS 55.0%
GGCAACTCAGAGGAAACTGG+TGG + chr4.2:65970282-65970301 MS.gene69174:CDS 55.0%
GGTGGCAACTCAGAGGAAAC+TGG + chr4.2:65970279-65970298 MS.gene69174:CDS 55.0%
!! AAACTGGTGGTGGCCAATGC+TGG + chr4.2:65970295-65970314 MS.gene69174:CDS 55.0%
GAAGCTCAGCCATCGCGAAC+CGG + chr4.2:65970251-65970270 MS.gene69174:CDS 60.0%
TCTGAGTTGCCACCGGAGAC+CGG - chr4.2:65970273-65970292 None:intergenic 60.0%
AGCCATCGCGAACCGGTCTC+CGG + chr4.2:65970258-65970277 MS.gene69174:CDS 65.0%
GGTCTCCGGTGGCAACTCAG+AGG + chr4.2:65970272-65970291 MS.gene69174:CDS 65.0%
CATCGCGAACCGGTCTCCGG+TGG + chr4.2:65970261-65970280 MS.gene69174:CDS 70.0%
!! CACCGGAGACCGGTTCGCGA+TGG - chr4.2:65970263-65970282 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 65970214 65970537 65970214 ID=MS.gene69174
chr4.2 mRNA 65970214 65970537 65970214 ID=MS.gene69174.t1;Parent=MS.gene69174
chr4.2 exon 65970214 65970537 65970214 ID=MS.gene69174.t1.exon1;Parent=MS.gene69174.t1
chr4.2 CDS 65970214 65970537 65970214 ID=cds.MS.gene69174.t1;Parent=MS.gene69174.t1
Gene Sequence

>MS.gene69174

ATGGCATCGATCACCATGACAGCAGCATTCTTCGGCGGAAGCTCAGCCATCGCGAACCGGTCTCCGGTGGCAACTCAGAGGAAACTGGTGGTGGCCAATGCTGGCAAAGCAGTTGAAGGAGGGAAAATGAAGGTGAGTTATGACAATGAAAAGGAAGAGACCAATGGAAGGAGGAACATCATGTTTGCTGCTGCAGCAGCAGCTGTTTGTTCTGTTGCTGGAATGGCTTTGGCTGATGAGCCAAAACGTGGTTCACCTGAAGCTAAGAAAGCCTATGCTCCTGTTTGTGTCACCATGCCAACAGCTAGAATTTGTCGCTACTAA

Protein sequence

>MS.gene69174.t1

MASITMTAAFFGGSSAIANRSPVATQRKLVVANAGKAVEGGKMKVSYDNEKEETNGRRNIMFAAAAAAVCSVAGMALADEPKRGSPEAKKAYAPVCVTMPTARICRY