AlfalfaGEDB | MS.gene057809

M. sativa cultivar XinJiangDaYe / MS.gene057809

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057809.t1	AES89342.1	95.6	91	4	0	1	91	1	91	1.40E-42	181.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057809.t1	P33083	75.6	90	22	0	1	90	1	90	3.0e-33	142.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057809.t1	G7JM99	95.6	91	4	0	1	91	1	91	1.0e-42	181.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054184	MS.gene057809	0.801217	9.99E-49	-1.69E-46
MS.gene054185	MS.gene057809	0.803225	3.86E-49	-1.69E-46
MS.gene057809	MS.gene063503	0.800064	1.72E-48	-1.69E-46
MS.gene057809	MS.gene38528	0.819274	1.27E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene057809	MS.gene68761	PPI
MS.gene057809	MS.gene40325	PPI
MS.gene057809	MS.gene68165	PPI
MS.gene057809	MS.gene75677	PPI
MS.gene057809	MS.gene50677	PPI
MS.gene057809	MS.gene79890	PPI
MS.gene057809	MS.gene50684	PPI
MS.gene057809	MS.gene057797	PPI
MS.gene057809	MS.gene045362	PPI
MS.gene057809	MS.gene23372	PPI
MS.gene057809	MS.gene014668	PPI
MS.gene057809	MS.gene79886	PPI
MS.gene057809	MS.gene50765	PPI
MS.gene057809	MS.gene014667	PPI
MS.gene057809	MS.gene062988	PPI
MS.gene057809	MS.gene003624	PPI
MS.gene057809	MS.gene003586	PPI
MS.gene003602	MS.gene057809	PPI
MS.gene057809	MS.gene003583	PPI
MS.gene057809	MS.gene003608	PPI
MS.gene057809	MS.gene77527	PPI
MS.gene057809	MS.gene003591	PPI
MS.gene057809	MS.gene79891	PPI
MS.gene057809	MS.gene045367	PPI
MS.gene057809	MS.gene045364	PPI
MS.gene057809	MS.gene045363	PPI
MS.gene057809	MS.gene062973	PPI
MS.gene062984	MS.gene057809	PPI
MS.gene057809	MS.gene50652	PPI
MS.gene057809	MS.gene014669	PPI
MS.gene057809	MS.gene057808	PPI
MS.gene057809	MS.gene002380	PPI
MS.gene057809	MS.gene77526	PPI
MS.gene057809	MS.gene77523	PPI
MS.gene057809	MS.gene045366	PPI
MS.gene003587	MS.gene057809	PPI
MS.gene057809	MS.gene68163	PPI
MS.gene057809	MS.gene057790	PPI
MS.gene057809	MS.gene75315	PPI
MS.gene003590	MS.gene057809	PPI
MS.gene057809	MS.gene057818	PPI
MS.gene057809	MS.gene68164	PPI
MS.gene057809	MS.gene062979	PPI
MS.gene057809	MS.gene68759	PPI
MS.gene057809	MS.gene79889	PPI
MS.gene057809	MS.gene003627	PPI
MS.gene057809	MS.gene031200	PPI
MS.gene057809	MS.gene045360	PPI
MS.gene057809	MS.gene014222	PPI
MS.gene057809	MS.gene79888	PPI
MS.gene057809	MS.gene75676	PPI
MS.gene057809	MS.gene40326	PPI
MS.gene057809	MS.gene75678	PPI
MS.gene057809	MS.gene031199	PPI
MS.gene057809	MS.gene50687	PPI
MS.gene057809	MS.gene014223	PPI
MS.gene057809	MS.gene79885	PPI
MS.gene003625	MS.gene057809	PPI
MS.gene057809	MS.gene047517	PPI
MS.gene057809	MS.gene01358	PPI
MS.gene057809	MS.gene062963	PPI
MS.gene057809	MS.gene063112	PPI
MS.gene057809	MS.gene50675	PPI
MS.gene057809	MS.gene045368	PPI
MS.gene057809	MS.gene40327	PPI
MS.gene057809	MS.gene045361	PPI
MS.gene062952	MS.gene057809	PPI
MS.gene057809	MS.gene75314	PPI
MS.gene057809	MS.gene01764	PPI
MS.gene057809	MS.gene75679	PPI
MS.gene23370	MS.gene057809	PPI
MS.gene50766	MS.gene057809	PPI
MS.gene77529	MS.gene057809	PPI
MS.gene003594	MS.gene057809	PPI
MS.gene057809	MS.gene75673	PPI
MS.gene057809	MS.gene057778	PPI
MS.gene50665	MS.gene057809	PPI
MS.gene057809	MS.gene68760	PPI
MS.gene057809	MS.gene031198	PPI
MS.gene057809	MS.gene50676	PPI
MS.gene057809	MS.gene003614	PPI
MS.gene057809	MS.gene50764	PPI
MS.gene057809	MS.gene77528	PPI
MS.gene057809	MS.gene75316	PPI
MS.gene057809	MS.gene014671	PPI
MS.gene057809	MS.gene003596	PPI
MS.gene057809	MS.gene003615	PPI
MS.gene057809	MS.gene003577	PPI
MS.gene057809	MS.gene23371	PPI
MS.gene057809	MS.gene75672	PPI
MS.gene50763	MS.gene057809	PPI
MS.gene057809	MS.gene75674	PPI
MS.gene045365	MS.gene057809	PPI
MS.gene057809	MS.gene50654	PPI
MS.gene057809	MS.gene062974	PPI
MS.gene50659	MS.gene057809	PPI
MS.gene057809	MS.gene014670	PPI
MS.gene057809	MS.gene062943	PPI
MS.gene057809	MS.gene062951	PPI
MS.gene057809	MS.gene057801	PPI
MS.gene057809	MS.gene77524	PPI
MS.gene057809	MS.gene057795	PPI
MS.gene057809	MS.gene68162	PPI
MS.gene057809	MS.gene75675	PPI
MS.gene057809	MS.gene79887	PPI
MS.gene031201	MS.gene057809	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057809.t1	MTR_4g072730	95.604	91	4	0	1	91	1	91	1.69e-62	184
MS.gene057809.t1	MTR_4g072620	89.130	92	10	0	1	92	1	92	8.41e-59	175
MS.gene057809.t1	MTR_4g072480	88.043	92	11	0	1	92	1	92	6.55e-58	172
MS.gene057809.t1	MTR_4g072660	89.130	92	5	1	1	92	1	87	6.60e-56	167
MS.gene057809.t1	MTR_4g072680	84.783	92	14	0	1	92	1	92	1.49e-55	167
MS.gene057809.t1	MTR_4g072460	82.609	92	16	0	1	92	1	92	1.02e-53	162
MS.gene057809.t1	MTR_4g072500	80.435	92	18	0	1	92	1	92	6.81e-52	157
MS.gene057809.t1	MTR_4g072640	77.778	90	20	0	1	90	1	90	1.72e-49	151
MS.gene057809.t1	MTR_4g072570	76.087	92	22	0	1	92	1	92	1.05e-48	149
MS.gene057809.t1	MTR_4g072530	76.667	90	21	0	1	90	1	90	1.28e-48	149
MS.gene057809.t1	MTR_3g084150	71.739	92	26	0	1	92	1	92	2.30e-46	144
MS.gene057809.t1	MTR_4g072270	70.968	93	26	1	1	92	1	93	8.29e-46	142
MS.gene057809.t1	MTR_4g072310	69.892	93	27	1	1	92	1	93	1.99e-45	141
MS.gene057809.t1	MTR_3g084180	70.652	92	27	0	1	92	1	92	3.82e-45	140
MS.gene057809.t1	MTR_4g072880	68.817	93	28	1	1	92	1	93	4.74e-45	140
MS.gene057809.t1	MTR_8g461410	69.565	92	28	0	1	92	1	92	5.67e-45	140
MS.gene057809.t1	MTR_4g072860	72.043	93	25	1	1	92	1	93	1.29e-44	139
MS.gene057809.t1	MTR_4g072240	70.330	91	26	1	1	90	1	91	2.17e-44	138
MS.gene057809.t1	MTR_4g072230	69.892	93	25	2	1	92	1	91	1.53e-43	136
MS.gene057809.t1	MTR_4g072250	70.968	93	25	2	1	92	1	92	6.85e-43	135
MS.gene057809.t1	MTR_4g072870	68.132	91	28	1	1	90	1	91	8.85e-43	134
MS.gene057809.t1	MTR_3g084240	67.391	92	30	0	1	92	1	92	1.56e-42	134
MS.gene057809.t1	MTR_4g072220	65.591	93	31	1	1	92	1	93	1.79e-42	134
MS.gene057809.t1	MTR_4g072300	65.934	91	30	1	1	90	1	91	2.18e-42	133
MS.gene057809.t1	MTR_3g084170	67.391	92	30	0	1	92	1	92	3.15e-42	133
MS.gene057809.t1	MTR_3g117630	73.256	86	23	0	7	92	7	92	4.67e-42	132
MS.gene057809.t1	MTR_3g110042	71.264	87	25	0	1	87	1	87	2.92e-41	130
MS.gene057809.t1	MTR_4g072330	67.778	90	27	1	1	90	1	88	8.33e-41	129
MS.gene057809.t1	MTR_3g110038	65.217	92	32	0	1	92	1	92	1.99e-40	129
MS.gene057809.t1	MTR_3g084220	66.304	92	31	0	1	92	1	92	2.15e-40	128
MS.gene057809.t1	MTR_4g072370	66.667	90	28	1	1	90	1	88	5.81e-40	127
MS.gene057809.t1	MTR_4g050700	65.591	93	31	1	1	92	1	93	5.81e-40	127
MS.gene057809.t1	MTR_2g012410	65.591	93	31	1	1	92	1	93	5.81e-40	127
MS.gene057809.t1	MTR_5g008360	75.000	80	20	0	13	92	4	83	9.24e-40	126
MS.gene057809.t1	MTR_3g084250	64.130	92	33	0	1	92	1	92	9.55e-40	127
MS.gene057809.t1	MTR_3g110032	65.591	93	31	1	1	92	1	93	1.35e-39	126
MS.gene057809.t1	MTR_4g072890	62.637	91	33	1	1	90	1	91	1.41e-39	126
MS.gene057809.t1	MTR_4g072260	61.290	93	35	1	1	92	1	93	1.51e-39	126
MS.gene057809.t1	MTR_4g072830	67.416	89	27	1	1	89	1	87	3.36e-39	125
MS.gene057809.t1	MTR_5g008370	78.378	74	16	0	18	91	18	91	4.04e-39	127
MS.gene057809.t1	MTR_3g084210	65.217	92	32	0	1	92	1	92	9.77e-39	124
MS.gene057809.t1	MTR_4g072550	79.452	73	15	0	20	92	31	103	1.06e-38	124
MS.gene057809.t1	MTR_4g072490	65.556	90	26	1	1	90	1	85	1.09e-38	124
MS.gene057809.t1	MTR_4g072630	65.556	90	27	1	1	90	1	86	1.33e-38	124
MS.gene057809.t1	MTR_4g072580	64.444	90	27	1	1	90	1	85	2.91e-38	123
MS.gene057809.t1	MTR_4g072740	66.667	90	25	1	1	90	1	85	3.35e-38	122
MS.gene057809.t1	MTR_4g072600	65.556	90	26	1	1	90	1	85	4.32e-38	122
MS.gene057809.t1	MTR_4g072850	65.957	94	30	1	1	92	1	94	6.90e-38	122
MS.gene057809.t1	MTR_3g084190	65.217	92	32	0	1	92	1	92	1.33e-37	121
MS.gene057809.t1	MTR_3g084260	62.366	93	34	1	1	92	1	93	2.05e-37	121
MS.gene057809.t1	MTR_4g072540	66.667	90	26	1	1	90	1	86	2.22e-37	120
MS.gene057809.t1	MTR_8g461420	64.444	90	32	0	1	90	1	90	6.10e-37	120
MS.gene057809.t1	MTR_4g072470	64.444	90	27	1	1	90	1	85	7.55e-37	119
MS.gene057809.t1	MTR_3g084160	64.130	92	33	0	1	92	1	92	1.75e-36	119
MS.gene057809.t1	MTR_3g084230	64.444	90	32	0	1	90	1	90	2.35e-36	118
MS.gene057809.t1	MTR_4g072465	60.000	90	32	1	1	90	1	86	6.74e-36	117
MS.gene057809.t1	MTR_4g072670	64.444	90	28	1	1	90	1	86	2.54e-35	115
MS.gene057809.t1	MTR_4g072720	64.444	90	28	1	1	90	1	86	3.20e-35	115
MS.gene057809.t1	MTR_4g072590	63.333	90	28	1	1	90	1	85	3.43e-35	115
MS.gene057809.t1	MTR_4g072610	64.444	90	26	1	1	90	1	84	4.25e-35	115
MS.gene057809.t1	MTR_4g072510	64.444	90	28	1	1	90	1	86	1.58e-34	113
MS.gene057809.t1	MTR_3g110045	63.441	93	32	1	2	92	59	151	2.51e-34	115
MS.gene057809.t1	MTR_4g072820	63.736	91	26	2	1	90	1	85	2.99e-34	113
MS.gene057809.t1	MTR_4g072940	72.222	72	20	0	21	92	53	124	3.23e-34	114
MS.gene057809.t1	MTR_4g072380	61.111	90	31	1	1	90	1	86	7.64e-34	112
MS.gene057809.t1	MTR_4g072650	60.000	90	30	1	1	90	1	84	1.42e-32	108
MS.gene057809.t1	MTR_4g072690	62.222	90	29	1	1	90	1	85	1.55e-31	106
MS.gene057809.t1	MTR_4g072290	56.989	93	25	2	1	92	1	79	2.22e-31	105
MS.gene057809.t1	MTR_5g008350	62.025	79	25	1	1	79	1	74	9.44e-31	103
MS.gene057809.t1	MTR_4g072450	58.889	90	32	1	1	90	1	85	1.18e-29	101
MS.gene057809.t1	MTR_4g072770	56.667	90	29	1	1	90	1	80	1.60e-29	100
MS.gene057809.t1	MTR_4g072980	52.688	93	38	2	1	92	8	95	1.68e-28	99.0
MS.gene057809.t1	MTR_4g072910	62.687	67	25	0	24	90	34	100	4.79e-28	97.8
MS.gene057809.t1	MTR_3g117640	67.647	68	21	1	25	91	34	101	5.10e-28	97.8
MS.gene057809.t1	MTR_4g072750	56.471	85	33	2	1	81	1	85	5.83e-28	97.1
MS.gene057809.t1	MTR_3g084200	59.722	72	29	0	1	72	1	72	6.77e-28	97.4
MS.gene057809.t1	MTR_4g072560	52.577	97	38	3	1	89	1	97	1.06e-27	97.1
MS.gene057809.t1	MTR_3g110035	66.667	66	22	0	27	92	73	138	1.19e-27	97.8
MS.gene057809.t1	MTR_4g072280	56.000	75	32	1	1	74	1	75	1.06e-25	91.3
MS.gene057809.t1	MTR_6g077850	53.731	67	31	0	24	90	33	99	7.24e-25	89.7
MS.gene057809.t1	MTR_3g113310	55.882	68	29	1	23	90	40	106	1.52e-24	89.0
MS.gene057809.t1	MTR_4g072190	51.163	86	40	2	5	90	17	100	1.43e-23	86.3
MS.gene057809.t1	MTR_3g117610	58.209	67	27	1	23	89	43	108	1.55e-23	86.7
MS.gene057809.t1	MTR_8g076040	47.500	80	41	1	11	90	27	105	1.22e-20	79.3
MS.gene057809.t1	MTR_4g072930	50.617	81	34	2	16	90	30	110	1.83e-20	79.0
MS.gene057809.t1	MTR_5g021820	50.746	67	32	1	24	90	39	104	3.22e-20	78.2
MS.gene057809.t1	MTR_8g461400	45.652	92	24	1	1	92	1	66	3.33e-19	74.3
MS.gene057809.t1	MTR_2g043960	45.333	75	40	1	15	89	66	139	8.88e-18	73.6
MS.gene057809.t1	MTR_4g124750	48.485	66	33	1	24	89	68	132	3.48e-17	71.6
MS.gene057809.t1	MTR_8g461210	40.698	86	50	1	4	89	14	98	3.57e-16	68.2
MS.gene057809.t1	MTR_4g072840	46.154	91	17	3	1	90	1	60	4.47e-16	66.2
MS.gene057809.t1	MTR_1g024085	45.783	83	41	2	4	83	13	94	8.08e-16	67.8
MS.gene057809.t1	MTR_4g005310	45.455	66	35	1	21	85	58	123	4.75e-15	66.2
MS.gene057809.t1	MTR_1g009150	50.820	61	29	1	25	85	71	130	2.26e-14	65.1
MS.gene057809.t1	MTR_7g118010	48.529	68	32	2	25	90	23	89	6.85e-14	62.0
MS.gene057809.t1	MTR_4g072160	48.571	70	33	2	17	85	29	96	7.43e-14	62.8
MS.gene057809.t1	MTR_1g094750	45.333	75	37	2	14	85	36	109	8.76e-14	62.0
MS.gene057809.t1	MTR_0388s0020	42.466	73	38	2	20	89	70	141	1.99e-13	62.0
MS.gene057809.t1	MTR_3g109160	47.761	67	34	1	25	91	69	134	2.24e-13	62.0
MS.gene057809.t1	MTR_4g025830	45.455	66	32	2	25	87	79	143	3.05e-13	61.6
MS.gene057809.t1	MTR_1g061570	44.118	68	37	1	24	91	18	84	6.15e-13	60.1
MS.gene057809.t1	MTR_4g005320	45.902	61	32	1	25	85	70	129	6.74e-13	60.8
MS.gene057809.t1	MTR_8g026730	45.588	68	34	2	25	90	27	93	6.78e-13	59.7
MS.gene057809.t1	MTR_3g092220	37.647	85	47	3	1	83	12	92	7.77e-13	59.7
MS.gene057809.t1	MTR_4g021810	42.857	77	39	3	10	85	14	86	2.55e-12	57.8
MS.gene057809.t1	MTR_1g059770	44.776	67	33	2	28	91	52	117	4.36e-12	57.8
MS.gene057809.t1	MTR_2g066830	37.079	89	49	2	2	90	41	122	5.70e-12	57.8
MS.gene057809.t1	MTR_3g098970	34.615	78	50	1	15	92	43	119	1.31e-11	57.0
MS.gene057809.t1	MTR_7g104940	41.935	62	35	1	28	89	53	113	2.56e-11	55.8
MS.gene057809.t1	MTR_3g097120	40.000	75	36	2	19	85	3	76	3.39e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057809.t1	AT4G38840	56.566	99	34	3	1	90	1	99	2.35e-31	106
MS.gene057809.t1	AT4G34770	56.667	90	37	1	4	91	14	103	3.59e-30	103
MS.gene057809.t1	AT4G38825	55.422	83	36	1	9	90	7	89	1.41e-28	99.0
MS.gene057809.t1	AT5G18020	52.273	88	40	2	1	87	1	87	2.41e-28	98.2
MS.gene057809.t1	AT5G18080	67.692	65	20	1	27	90	26	90	3.26e-28	97.8
MS.gene057809.t1	AT2G21210	50.526	95	42	2	1	90	1	95	5.14e-28	97.8
MS.gene057809.t1	AT5G18060	66.154	65	21	1	27	90	26	90	6.94e-28	97.1
MS.gene057809.t1	AT3G03840	54.945	91	36	2	3	91	8	95	7.40e-28	97.1
MS.gene057809.t1	AT4G34800	54.118	85	32	2	1	82	1	81	7.60e-28	97.1
MS.gene057809.t1	AT3G03820	57.471	87	31	2	8	91	13	96	1.18e-27	96.7
MS.gene057809.t1	AT2G21200	63.380	71	25	1	20	89	16	86	1.24e-27	96.3
MS.gene057809.t1	AT5G18030	58.108	74	30	1	18	90	15	88	1.82e-27	95.9
MS.gene057809.t1	AT3G03850	58.696	92	29	3	3	91	8	93	2.72e-27	95.9
MS.gene057809.t1	AT2G21210	50.526	95	42	2	1	90	68	162	2.90e-27	97.8
MS.gene057809.t1	AT5G18050	64.615	65	22	1	27	90	26	90	2.98e-27	95.5
MS.gene057809.t1	AT4G38850	61.111	72	25	1	19	87	15	86	1.43e-26	93.6
MS.gene057809.t1	AT5G18010	49.451	91	44	2	1	90	1	90	4.70e-26	92.4
MS.gene057809.t1	AT4G34810	64.516	62	18	1	25	82	48	109	1.65e-25	92.0
MS.gene057809.t1	AT3G03830	53.409	88	32	2	3	87	8	89	3.32e-24	87.8
MS.gene057809.t1	AT4G13790	55.714	70	29	1	20	87	20	89	1.22e-23	86.3
MS.gene057809.t1	AT4G38860	57.353	68	29	0	23	90	38	105	2.12e-23	86.3
MS.gene057809.t1	AT1G75580	58.824	68	27	1	23	90	42	108	1.64e-22	84.0
MS.gene057809.t1	AT2G21220	56.716	67	28	1	23	89	38	103	2.04e-22	83.6
MS.gene057809.t1	AT4G34790	58.462	65	25	1	25	87	39	103	2.22e-22	83.6
MS.gene057809.t1	AT4G34760	55.882	68	29	1	23	90	41	107	2.33e-22	83.6
MS.gene057809.t1	AT4G34780	57.746	71	27	1	23	90	26	96	4.62e-22	82.8
MS.gene057809.t1	AT2G16580	51.471	68	32	1	23	90	42	108	5.17e-19	75.1
MS.gene057809.t1	AT1G19830	54.930	71	30	2	20	89	43	112	9.31e-19	74.7
MS.gene057809.t1	AT3G43120	40.230	87	51	1	5	91	59	144	1.19e-18	75.5
MS.gene057809.t1	AT4G36110	53.125	64	29	1	24	87	39	101	3.78e-18	72.8
MS.gene057809.t1	AT2G18010	51.562	64	30	1	24	87	47	109	2.24e-17	71.2
MS.gene057809.t1	AT5G20810	48.485	66	33	1	24	89	78	142	9.47e-17	70.9
MS.gene057809.t1	AT5G20810	45.205	73	39	1	17	89	71	142	1.00e-16	71.2
MS.gene057809.t1	AT5G66260	45.679	81	38	2	15	90	19	98	3.75e-16	67.8
MS.gene057809.t1	AT3G20220	50.000	62	29	2	25	85	47	107	3.79e-16	68.2
MS.gene057809.t1	AT1G75590	43.529	85	42	2	4	83	16	99	1.93e-15	67.4
MS.gene057809.t1	AT5G10990	49.275	69	34	1	15	83	31	98	8.54e-15	65.5
MS.gene057809.t1	AT3G12830	40.230	87	50	2	4	90	30	114	1.60e-14	64.3
MS.gene057809.t1	AT3G51200	48.214	56	28	1	27	82	42	96	4.66e-14	62.4
MS.gene057809.t1	AT1G19840	49.275	69	34	1	15	83	31	98	7.26e-14	63.2
MS.gene057809.t1	AT3G61900	43.590	78	40	2	16	90	21	97	1.97e-13	61.6
MS.gene057809.t1	AT5G53590	45.833	72	33	2	24	90	45	115	2.35e-13	61.6
MS.gene057809.t1	AT4G34750	45.312	64	34	1	24	87	36	98	4.96e-13	60.8
MS.gene057809.t1	AT4G34750	45.312	64	34	1	24	87	36	98	4.96e-13	60.8
MS.gene057809.t1	AT2G46690	38.372	86	49	2	9	91	8	92	8.25e-13	59.7
MS.gene057809.t1	AT3G60690	41.667	72	38	2	19	87	82	152	8.44e-13	60.8
MS.gene057809.t1	AT3G09870	48.438	64	30	2	24	85	44	106	2.32e-12	58.2
MS.gene057809.t1	AT2G45210	40.845	71	38	2	20	87	74	143	5.14e-12	58.5
MS.gene057809.t1	AT3G03847	42.105	76	41	3	8	82	54	127	5.77e-12	57.8
MS.gene057809.t1	AT2G37030	40.000	65	38	1	24	88	48	111	1.03e-11	57.0
MS.gene057809.t1	AT1G56150	42.105	76	42	2	15	90	29	102	1.12e-11	56.6
MS.gene057809.t1	AT4G00880	43.478	69	36	2	24	90	27	94	2.18e-11	55.8
MS.gene057809.t1	AT4G09530	39.706	68	38	2	27	91	30	97	2.76e-11	55.5
MS.gene057809.t1	AT1G16510	42.424	66	37	1	25	90	54	118	5.53e-11	55.5
MS.gene057809.t1	AT4G31320	44.262	61	33	1	25	85	80	139	6.08e-11	56.2

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAACTTCTTGCTTGAA+TGG	0.302470	4.4:+36718564	MS.gene057809:CDS
ATTTCAAGACTTGTTGAATC+AGG	0.319811	4.4:+36718470	MS.gene057809:CDS
AATCAGGCTGAAGAAGAGTT+TGG	0.367604	4.4:+36718486	MS.gene057809:CDS
AAATCTGTAGAAGTGATAAA+AGG	0.369929	4.4:+36718375	MS.gene057809:CDS
CGTTTACACACTATTCTAAA+GGG	0.375968	4.4:+36718324	MS.gene057809:CDS
GGTTGGTTTAAGTATGAAAC+AGG	0.401861	4.4:-36718444	None:intergenic
CCGTTTACACACTATTCTAA+AGG	0.405819	4.4:+36718323	MS.gene057809:CDS
TTCAACAAGTCTTGAAATGA+AGG	0.413456	4.4:-36718465	None:intergenic
ATATAACTTCTTGCTTGAAT+GGG	0.446395	4.4:+36718565	MS.gene057809:CDS
CCTTTAGAATAGTGTGTAAA+CGG	0.450829	4.4:-36718323	None:intergenic
GGCTATGTTGCAGCGTATGT+TGG	0.486291	4.4:+36718396	MS.gene057809:CDS
ACAAGTCTTGAAATGAAGGT+TGG	0.490149	4.4:-36718461	None:intergenic
TCAAGCAAGAAGTTATATGT+TGG	0.522465	4.4:-36718560	None:intergenic
TTTGGATATGATCATCCAAT+GGG	0.545525	4.4:+36718504	MS.gene057809:CDS
GACATCTTCGGAGCAAGGAA+TGG	0.562238	4.4:-36718535	None:intergenic
GGAATGGTGAGGCCACCCAT+TGG	0.562484	4.4:-36718519	None:intergenic
TATATGTTGGAAGACATCTT+CGG	0.572640	4.4:-36718547	None:intergenic
GTTTGGATATGATCATCCAA+TGG	0.573812	4.4:+36718503	MS.gene057809:CDS
TGGAAGACATCTTCGGAGCA+AGG	0.586129	4.4:-36718540	None:intergenic
GGATATGATCATCCAATGGG+TGG	0.648071	4.4:+36718507	MS.gene057809:CDS
CTTCGGAGCAAGGAATGGTG+AGG	0.674250	4.4:-36718530	None:intergenic
ATGTTGGAGAGAAACTGACA+CGG	0.761624	4.4:+36718412	MS.gene057809:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATCTGTAGAAGTGATAAA+AGG	+	chr4.4:36718375-36718394	MS.gene057809:CDS	25.0%
!	ATATAACTTCTTGCTTGAAT+GGG	+	chr4.4:36718565-36718584	MS.gene057809:CDS	25.0%
	CATATAACTTCTTGCTTGAA+TGG	+	chr4.4:36718564-36718583	MS.gene057809:CDS	30.0%
	CGTTTACACACTATTCTAAA+GGG	+	chr4.4:36718324-36718343	MS.gene057809:CDS	30.0%
	TATATGTTGGAAGACATCTT+CGG	-	chr4.4:36718550-36718569	None:intergenic	30.0%
	TCAAGCAAGAAGTTATATGT+TGG	-	chr4.4:36718563-36718582	None:intergenic	30.0%
	TTCAACAAGTCTTGAAATGA+AGG	-	chr4.4:36718468-36718487	None:intergenic	30.0%
	TTTGGATATGATCATCCAAT+GGG	+	chr4.4:36718504-36718523	MS.gene057809:CDS	30.0%
!!	ATTTCAAGACTTGTTGAATC+AGG	+	chr4.4:36718470-36718489	MS.gene057809:CDS	30.0%
!!	CCTTTAGAATAGTGTGTAAA+CGG	-	chr4.4:36718326-36718345	None:intergenic	30.0%
	ACAAGTCTTGAAATGAAGGT+TGG	-	chr4.4:36718464-36718483	None:intergenic	35.0%
	CCGTTTACACACTATTCTAA+AGG	+	chr4.4:36718323-36718342	MS.gene057809:CDS	35.0%
	GTTTGGATATGATCATCCAA+TGG	+	chr4.4:36718503-36718522	MS.gene057809:CDS	35.0%
!!	GGTTGGTTTAAGTATGAAAC+AGG	-	chr4.4:36718447-36718466	None:intergenic	35.0%
	AATCAGGCTGAAGAAGAGTT+TGG	+	chr4.4:36718486-36718505	MS.gene057809:CDS	40.0%
	ATGTTGGAGAGAAACTGACA+CGG	+	chr4.4:36718412-36718431	MS.gene057809:CDS	40.0%
	GGATATGATCATCCAATGGG+TGG	+	chr4.4:36718507-36718526	MS.gene057809:CDS	45.0%
	GACATCTTCGGAGCAAGGAA+TGG	-	chr4.4:36718538-36718557	None:intergenic	50.0%
	GGCTATGTTGCAGCGTATGT+TGG	+	chr4.4:36718396-36718415	MS.gene057809:CDS	50.0%
	TGGAAGACATCTTCGGAGCA+AGG	-	chr4.4:36718543-36718562	None:intergenic	50.0%
	CTTCGGAGCAAGGAATGGTG+AGG	-	chr4.4:36718533-36718552	None:intergenic	55.0%
	GGAATGGTGAGGCCACCCAT+TGG	-	chr4.4:36718522-36718541	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36718315	36718593	36718315	ID=MS.gene057809
chr4.4	mRNA	36718315	36718593	36718315	ID=MS.gene057809.t1;Parent=MS.gene057809
chr4.4	exon	36718315	36718593	36718315	ID=MS.gene057809.t1.exon1;Parent=MS.gene057809.t1
chr4.4	CDS	36718315	36718593	36718315	ID=cds.MS.gene057809.t1;Parent=MS.gene057809.t1

Gene Sequence	>MS.gene057809 ATGGGTTTCCGTTTACACACTATTCTAAAGGGTTCAGTAACTTCTAGTCAAGCAAAATCAAAATCTGTAGAAGTGATAAAAGGCTATGTTGCAGCGTATGTTGGAGAGAAACTGACACGGTTTGTAGTTCCTGTTTCATACTTAAACCAACCTTCATTTCAAGACTTGTTGAATCAGGCTGAAGAAGAGTTTGGATATGATCATCCAATGGGTGGCCTCACCATTCCTTGCTCCGAAGATGTCTTCCAACATATAACTTCTTGCTTGAATGGGCTATGA
Protein sequence	>MS.gene057809.t1 MGFRLHTILKGSVTSSQAKSKSVEVIKGYVAAYVGEKLTRFVVPVSYLNQPSFQDLLNQAEEEFGYDHPMGGLTIPCSEDVFQHITSCLNGL