AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06990.t1 MTR_7g108790 94.972 179 9 0 17 195 17 195 5.17e-124 348
MS.gene06990.t1 MTR_1g088540 69.536 151 46 0 48 198 1 151 6.19e-75 222
MS.gene06990.t1 MTR_5g012000 34.043 188 114 3 8 190 28 210 1.25e-31 114
MS.gene06990.t1 MTR_4g120825 32.338 201 123 4 5 192 6 206 2.75e-31 113
MS.gene06990.t1 MTR_4g120830 32.338 201 123 4 5 192 6 206 4.27e-31 112
MS.gene06990.t1 MTR_4g035905 31.282 195 124 3 3 190 4 195 7.80e-31 112
MS.gene06990.t1 MTR_3g008840 33.333 168 108 3 26 189 18 185 7.12e-30 108
MS.gene06990.t1 MTR_8g104765 33.140 172 103 3 34 193 45 216 1.22e-29 109
MS.gene06990.t1 MTR_4g035880 30.729 192 129 2 3 190 4 195 2.58e-29 107
MS.gene06990.t1 MTR_4g035870 29.688 192 131 2 3 190 4 195 1.13e-27 103
MS.gene06990.t1 MTR_3g008820 29.762 168 114 3 26 189 17 184 2.93e-26 99.8
MS.gene06990.t1 MTR_2g043560 32.768 177 107 4 19 190 20 189 2.26e-25 97.8
MS.gene06990.t1 MTR_4g101760 30.890 191 124 3 7 189 10 200 1.22e-24 95.9
MS.gene06990.t1 MTR_4g025550 29.858 211 124 6 1 196 5 206 5.73e-22 89.4
MS.gene06990.t1 MTR_4g124855 30.928 194 120 6 3 187 8 196 1.97e-21 87.8
MS.gene06990.t1 MTR_4g025590 30.178 169 103 4 34 189 4 170 2.45e-19 81.6
MS.gene06990.t1 MTR_4g025610 29.384 211 127 7 3 196 1 206 4.71e-19 81.6
MS.gene06990.t1 MTR_4g025620 29.412 204 122 7 3 189 1 199 5.08e-19 81.6
MS.gene06990.t1 MTR_7g112580 29.487 156 107 3 34 187 40 194 6.21e-17 78.6
MS.gene06990.t1 MTR_7g108905 30.366 191 120 3 7 188 15 201 1.41e-16 77.4
MS.gene06990.t1 MTR_8g023310 32.903 155 99 4 34 183 68 222 2.23e-15 73.9
MS.gene06990.t1 MTR_1g008140 28.804 184 106 5 17 183 67 242 8.24e-15 72.4
MS.gene06990.t1 MTR_4g120290 33.333 159 98 5 33 189 58 210 1.00e-14 72.0
MS.gene06990.t1 MTR_3g091380 27.273 165 106 2 29 183 73 233 2.05e-14 71.2
MS.gene06990.t1 MTR_3g008760 26.582 158 109 4 34 185 67 223 6.96e-14 68.2
MS.gene06990.t1 MTR_8g042900 29.487 156 102 3 34 184 76 228 9.51e-14 69.3
MS.gene06990.t1 MTR_2g436040 30.380 158 102 5 33 187 42 194 2.17e-13 68.2
MS.gene06990.t1 MTR_0036s0140 28.729 181 117 5 7 184 50 221 5.49e-13 65.5
MS.gene06990.t1 MTR_2g436070 28.125 160 105 3 29 185 51 203 8.23e-13 66.2
MS.gene06990.t1 MTR_4g120280 29.487 156 106 1 34 189 59 210 9.80e-13 66.2
MS.gene06990.t1 MTR_3g010780 28.205 156 107 3 32 184 69 222 2.13e-12 65.5
MS.gene06990.t1 MTR_7g112590 26.506 166 117 4 34 198 59 220 3.42e-12 64.7
MS.gene06990.t1 MTR_2g436050 27.778 162 103 4 29 185 51 203 3.78e-12 64.7
MS.gene06990.t1 MTR_1g083510 27.329 161 113 3 34 193 56 213 6.08e-12 63.9
MS.gene06990.t1 MTR_1g083470 26.994 163 107 3 34 190 58 214 2.72e-11 62.0
MS.gene06990.t1 MTR_1g083500 26.994 163 107 3 34 190 58 214 2.72e-11 62.0
MS.gene06990.t1 MTR_4g124550 31.325 166 102 6 34 195 81 238 4.87e-11 61.2
MS.gene06990.t1 MTR_8g023150 27.500 160 107 4 33 187 76 231 5.27e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06990.t1 AT3G62820 44.560 193 101 3 3 192 5 194 5.37e-53 168
MS.gene06990.t1 AT2G47670 47.531 162 80 3 34 192 49 208 3.61e-39 133
MS.gene06990.t1 AT5G62360 34.177 158 101 3 34 189 44 200 4.01e-30 110
MS.gene06990.t1 AT5G20740 33.684 190 118 4 9 190 7 196 6.33e-30 109
MS.gene06990.t1 AT5G20740 33.684 190 118 4 9 190 41 230 1.37e-29 109
MS.gene06990.t1 AT4G12390 35.758 165 99 2 32 189 38 202 1.97e-28 105
MS.gene06990.t1 AT1G62770 32.632 190 119 5 8 188 7 196 1.70e-27 103
MS.gene06990.t1 AT5G62350 32.121 165 106 3 32 190 37 201 6.70e-27 102
MS.gene06990.t1 AT4G25260 31.795 195 117 5 7 189 9 199 3.10e-26 100
MS.gene06990.t1 AT2G01610 31.818 176 102 4 34 191 45 220 1.32e-24 96.7
MS.gene06990.t1 AT1G62760 31.953 169 112 1 25 190 139 307 1.52e-24 98.2
MS.gene06990.t1 AT1G14890 33.333 168 94 4 34 191 59 218 4.75e-24 95.1
MS.gene06990.t1 AT1G70720 32.990 194 114 5 11 192 9 198 1.66e-22 90.5
MS.gene06990.t1 AT1G70720 32.353 204 120 6 1 192 13 210 2.20e-22 90.5
MS.gene06990.t1 AT1G23205 31.053 190 119 4 9 186 9 198 3.25e-22 89.7
MS.gene06990.t1 AT3G47380 30.061 163 108 3 32 188 37 199 7.24e-22 89.0
MS.gene06990.t1 AT3G14310 35.542 166 97 6 34 192 59 221 3.92e-21 90.9
MS.gene06990.t1 AT4G25250 27.660 188 122 5 8 184 9 193 7.21e-17 75.5
MS.gene06990.t1 AT3G05620 28.079 203 128 5 1 187 1 201 7.29e-17 78.6
MS.gene06990.t1 AT5G51520 30.256 195 120 8 9 187 8 202 3.02e-16 73.9
MS.gene06990.t1 AT3G05610 29.487 156 106 3 34 188 58 210 9.38e-16 75.1
MS.gene06990.t1 AT1G53830 32.530 166 102 6 34 192 62 224 3.65e-15 73.6
MS.gene06990.t1 AT4G00080 28.221 163 105 5 34 185 36 197 5.49e-15 70.9
MS.gene06990.t1 AT3G49220 30.921 152 97 4 34 183 132 277 2.07e-14 71.2
MS.gene06990.t1 AT5G04960 30.065 153 95 3 33 184 67 208 3.49e-14 70.5
MS.gene06990.t1 AT3G14300 27.807 187 123 5 13 188 250 435 4.97e-14 70.5
MS.gene06990.t1 AT3G49220 30.921 152 97 4 34 183 132 277 5.35e-14 70.1
MS.gene06990.t1 AT3G49220 30.921 152 97 4 34 183 87 232 5.94e-14 70.1
MS.gene06990.t1 AT5G27870 25.806 155 113 2 34 188 57 209 2.21e-13 68.6
MS.gene06990.t1 AT5G53370 29.412 153 101 3 34 183 111 259 3.30e-12 65.1
MS.gene06990.t1 AT5G53370 29.412 153 101 3 34 183 75 223 4.65e-12 64.3
MS.gene06990.t1 AT4G15980 27.273 154 105 3 38 187 52 202 7.74e-12 63.9
MS.gene06990.t1 AT4G15980 27.273 154 105 3 38 187 62 212 8.95e-12 63.5
MS.gene06990.t1 AT1G53840 28.144 167 109 4 33 189 72 237 5.48e-11 61.2
MS.gene06990.t1 AT3G10710 27.551 196 122 7 1 188 33 216 8.29e-11 60.8
MS.gene06990.t1 AT5G49180 28.497 193 124 6 2 184 17 205 8.99e-11 60.8

Find 34 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAAATTGCTGCAAAATA+AGG 0.253916 7.3:-6259023 MS.gene06990:CDS
CGGCACGTGCCCATTTGCTT+GGG 0.271742 7.3:+6259326 None:intergenic
TAAACTTGCTTCTACGGGTT+TGG 0.307810 7.3:-6258954 MS.gene06990:CDS
CCGGCACGTGCCCATTTGCT+TGG 0.317654 7.3:+6259325 None:intergenic
TCAGATTGCATAGAAACTTT+TGG 0.319992 7.3:-6259205 MS.gene06990:CDS
GAAATACTAATAGTAAAATA+AGG 0.351810 7.3:+6259491 None:intergenic
AATGAAGACACTTGCCTTGA+TGG 0.365286 7.3:-6259070 MS.gene06990:CDS
CAAGCAAATGGGCACGTGCC+GGG 0.387799 7.3:-6259324 MS.gene06990:CDS
CAGGAAGAACCCCAAGCAAA+TGG 0.388875 7.3:-6259336 MS.gene06990:CDS
AAACACATTTAGTACACAAA+TGG 0.389035 7.3:-6259128 MS.gene06990:CDS
TGTCGATAAACTTGCTTCTA+CGG 0.397220 7.3:-6258960 MS.gene06990:CDS
GTGTGTGGATGCAAAGGTTT+TGG 0.415849 7.3:+6259380 None:intergenic
TGGGGTTCTTCCTGCTGTGT+TGG 0.420102 7.3:+6259345 None:intergenic
ATGCACTTGATGATCTTCAT+AGG 0.431401 7.3:-6259180 MS.gene06990:CDS
ACTTGCCTTGATGGGTTTGA+AGG 0.437348 7.3:-6259061 MS.gene06990:CDS
AAACAAGAACAACAATGTTA+AGG 0.453205 7.3:-6259425 MS.gene06990:CDS
GTCGATAAACTTGCTTCTAC+GGG 0.454014 7.3:-6258959 MS.gene06990:CDS
AGCCAAATTGTATATGCAAA+AGG 0.474317 7.3:-6259448 MS.gene06990:CDS
ATAGGTCACTTGGTGTGCTT+AGG 0.475642 7.3:-6259162 MS.gene06990:CDS
AACACATTTAGTACACAAAT+GGG 0.486915 7.3:-6259127 MS.gene06990:CDS
CCAAGCAAATGGGCACGTGC+CGG 0.491123 7.3:-6259325 MS.gene06990:CDS
AGGAAGAACCCCAAGCAAAT+GGG 0.506154 7.3:-6259335 MS.gene06990:CDS
AAGCAAATGGGCACGTGCCG+GGG 0.507156 7.3:-6259323 MS.gene06990:CDS
ATGAAGACACTTGCCTTGAT+GGG 0.520738 7.3:-6259069 MS.gene06990:CDS
ATGGGCACGTGCCGGGGTGT+CGG 0.523182 7.3:-6259317 MS.gene06990:CDS
AAGATGAACGAGAAGATGAA+AGG 0.537634 7.3:-6259247 MS.gene06990:CDS
GGCACGTGCCCATTTGCTTG+GGG 0.543135 7.3:+6259327 None:intergenic
GCACAATTCTCCAACACAGC+AGG 0.560783 7.3:-6259355 MS.gene06990:CDS
GATGATCTTCATAGGTCACT+TGG 0.570132 7.3:-6259172 MS.gene06990:CDS
TGGAGAATTGTGCTAGTGTG+TGG 0.572339 7.3:+6259365 None:intergenic
GTGCTAGTGTGTGGATGCAA+AGG 0.620695 7.3:+6259374 None:intergenic
AGGAAGCTTGCAGTGTCACA+AGG 0.623585 7.3:-6259405 MS.gene06990:CDS
AAATGGGAGATCTCAACACA+TGG 0.662660 7.3:-6259111 MS.gene06990:CDS
GCTTATCGTTACCGACACCC+CGG 0.696996 7.3:+6259306 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATACTAATAGTAAAATA+AGG + chr7.3:6258954-6258973 None:intergenic 15.0%
!! AATTAAATTGCTGCAAAATA+AGG - chr7.3:6259419-6259438 MS.gene06990:CDS 20.0%
! AAACAAGAACAACAATGTTA+AGG - chr7.3:6259017-6259036 MS.gene06990:CDS 25.0%
! AAACACATTTAGTACACAAA+TGG - chr7.3:6259314-6259333 MS.gene06990:CDS 25.0%
! AACACATTTAGTACACAAAT+GGG - chr7.3:6259315-6259334 MS.gene06990:CDS 25.0%
!! TTCCTTTTGCATATACAATT+TGG + chr7.3:6258999-6259018 None:intergenic 25.0%
AGCCAAATTGTATATGCAAA+AGG - chr7.3:6258994-6259013 MS.gene06990:CDS 30.0%
! TCAGATTGCATAGAAACTTT+TGG - chr7.3:6259237-6259256 MS.gene06990:CDS 30.0%
AAGATGAACGAGAAGATGAA+AGG - chr7.3:6259195-6259214 MS.gene06990:CDS 35.0%
! ATGCACTTGATGATCTTCAT+AGG - chr7.3:6259262-6259281 MS.gene06990:CDS 35.0%
! TGTCGATAAACTTGCTTCTA+CGG - chr7.3:6259482-6259501 MS.gene06990:CDS 35.0%
! TTTTTCCTTCAAACCCATCA+AGG + chr7.3:6259389-6259408 None:intergenic 35.0%
!! TGATGGGTTTGAAGGAAAAA+CGG - chr7.3:6259389-6259408 MS.gene06990:CDS 35.0%
AAATGGGAGATCTCAACACA+TGG - chr7.3:6259331-6259350 MS.gene06990:CDS 40.0%
AATGAAGACACTTGCCTTGA+TGG - chr7.3:6259372-6259391 MS.gene06990:CDS 40.0%
ATGAAGACACTTGCCTTGAT+GGG - chr7.3:6259373-6259392 MS.gene06990:CDS 40.0%
GATGATCTTCATAGGTCACT+TGG - chr7.3:6259270-6259289 MS.gene06990:CDS 40.0%
! GTCGATAAACTTGCTTCTAC+GGG - chr7.3:6259483-6259502 MS.gene06990:CDS 40.0%
! TAAACTTGCTTCTACGGGTT+TGG - chr7.3:6259488-6259507 MS.gene06990:CDS 40.0%
AGGAAGAACCCCAAGCAAAT+GGG - chr7.3:6259107-6259126 MS.gene06990:CDS 45.0%
TGGAGAATTGTGCTAGTGTG+TGG + chr7.3:6259080-6259099 None:intergenic 45.0%
! GTGTGTGGATGCAAAGGTTT+TGG + chr7.3:6259065-6259084 None:intergenic 45.0%
!! ACTTGCCTTGATGGGTTTGA+AGG - chr7.3:6259381-6259400 MS.gene06990:CDS 45.0%
!! ATAGGTCACTTGGTGTGCTT+AGG - chr7.3:6259280-6259299 MS.gene06990:CDS 45.0%
AGGAAGCTTGCAGTGTCACA+AGG - chr7.3:6259037-6259056 MS.gene06990:CDS 50.0%
CAGGAAGAACCCCAAGCAAA+TGG - chr7.3:6259106-6259125 MS.gene06990:CDS 50.0%
GCACAATTCTCCAACACAGC+AGG - chr7.3:6259087-6259106 MS.gene06990:CDS 50.0%
GTGCTAGTGTGTGGATGCAA+AGG + chr7.3:6259071-6259090 None:intergenic 50.0%
GCTTATCGTTACCGACACCC+CGG + chr7.3:6259139-6259158 None:intergenic 55.0%
TGGGGTTCTTCCTGCTGTGT+TGG + chr7.3:6259100-6259119 None:intergenic 55.0%
AAGCAAATGGGCACGTGCCG+GGG - chr7.3:6259119-6259138 MS.gene06990:CDS 60.0%
CAAGCAAATGGGCACGTGCC+GGG - chr7.3:6259118-6259137 MS.gene06990:CDS 60.0%
CCAAGCAAATGGGCACGTGC+CGG - chr7.3:6259117-6259136 MS.gene06990:CDS 60.0%
CGGCACGTGCCCATTTGCTT+GGG + chr7.3:6259119-6259138 None:intergenic 60.0%
GGCACGTGCCCATTTGCTTG+GGG + chr7.3:6259118-6259137 None:intergenic 60.0%
CCGGCACGTGCCCATTTGCT+TGG + chr7.3:6259120-6259139 None:intergenic 65.0%
ATGGGCACGTGCCGGGGTGT+CGG - chr7.3:6259125-6259144 MS.gene06990:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 6258934 6259530 6258934 ID=MS.gene06990
chr7.3 mRNA 6258934 6259530 6258934 ID=MS.gene06990.t1;Parent=MS.gene06990
chr7.3 exon 6258934 6259530 6258934 ID=MS.gene06990.t1.exon1;Parent=MS.gene06990.t1
chr7.3 CDS 6258934 6259530 6258934 ID=cds.MS.gene06990.t1;Parent=MS.gene06990.t1
Gene Sequence

>MS.gene06990

ATGATTATAACATCACGCCTTATTTTACTATTAGTATTTCTTTCATTCTCAACAAATACTAGCCAAATTGTATATGCAAAAGGAAACAAGAACAACAATGTTAAGGAAGCTTGCAGTGTCACAAGGTACCAAAACCTTTGCATCCACACACTAGCACAATTCTCCAACACAGCAGGAAGAACCCCAAGCAAATGGGCACGTGCCGGGGTGTCGGTAACGATAAGCGAAGTTAAGAATGTTCAAGCATATCTCACAAAATTAAAGATGAACGAGAAGATGAAAGGAAGAAACAGAGTTGCACTTTCAGATTGCATAGAAACTTTTGGCTATGCACTTGATGATCTTCATAGGTCACTTGGTGTGCTTAGGAAACTAAGCAAAAACACATTTAGTACACAAATGGGAGATCTCAACACATGGATTAGTGCAGCTCTTACTAATGAAGACACTTGCCTTGATGGGTTTGAAGGAAAAACGGAGAAGCAAATTAAATTGCTGCAAAATAAGGTTAAGAACGTGTCTTACATAACAAGTAACGCACTTGCTCTTGTCGATAAACTTGCTTCTACGGGTTTGGAAAGCATCACTGATCCATAG

Protein sequence

>MS.gene06990.t1

MIITSRLILLLVFLSFSTNTSQIVYAKGNKNNNVKEACSVTRYQNLCIHTLAQFSNTAGRTPSKWARAGVSVTISEVKNVQAYLTKLKMNEKMKGRNRVALSDCIETFGYALDDLHRSLGVLRKLSKNTFSTQMGDLNTWISAALTNEDTCLDGFEGKTEKQIKLLQNKVKNVSYITSNALALVDKLASTGLESITDP