AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070955


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070955.t1 MTR_1g102890 97.778 180 4 0 1 180 8 187 6.68e-131 364
MS.gene070955.t1 MTR_1g102890 95.070 142 7 0 1 142 8 149 2.62e-98 281
MS.gene070955.t1 MTR_1g102870 67.442 172 56 0 1 172 18 189 4.20e-89 259
MS.gene070955.t1 MTR_1g102870 69.784 139 42 0 1 139 18 156 6.89e-73 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070955.t1 AT5G43850 78.333 180 39 0 1 180 8 187 3.33e-105 300
MS.gene070955.t1 AT2G26400 66.092 174 59 0 1 174 33 206 1.88e-87 256
MS.gene070955.t1 AT4G14710 64.912 171 60 0 1 171 1 171 2.41e-87 254
MS.gene070955.t1 AT2G26400 66.092 174 59 0 1 174 18 191 2.61e-87 254
MS.gene070955.t1 AT4G14710 64.912 171 60 0 1 171 18 188 3.79e-87 254
MS.gene070955.t1 AT4G14710 64.912 171 60 0 1 171 18 188 3.79e-87 254
MS.gene070955.t1 AT4G14710 64.912 171 60 0 1 171 20 190 3.96e-87 254
MS.gene070955.t1 AT4G14716 65.663 166 57 0 1 166 1 166 9.30e-86 250
MS.gene070955.t1 AT4G14716 65.663 166 57 0 1 166 18 183 1.59e-85 250
MS.gene070955.t1 AT4G14710 64.535 172 60 1 1 171 18 189 2.74e-85 249
MS.gene070955.t1 AT2G26400 68.519 162 51 0 1 162 33 194 8.58e-85 249
MS.gene070955.t1 AT2G26400 68.519 162 51 0 1 162 18 179 9.53e-85 249
MS.gene070955.t1 AT4G14710 66.049 162 55 0 1 162 18 179 3.11e-84 246

Find 44 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTAGAAAGGAATACATTA+AGG 0.274694 1.4:-79568595 MS.gene070955:CDS
TGATCATTCTCATAGTCATT+AGG 0.279982 1.4:+79569812 None:intergenic
GATCATTCTCATAGTCATTA+GGG 0.297534 1.4:+79569813 None:intergenic
GCAGTTAATGAGGTTGTTTA+TGG 0.306900 1.4:-79568664 MS.gene070955:intron
CCCCAATGAATTTGTTTCCT+TGG 0.318267 1.4:-79569960 MS.gene070955:CDS
TGTTCCACTTGCAGCTCATT+AGG 0.322239 1.4:-79568553 None:intergenic
ATGATTAATAACCTGCTAAT+TGG 0.327623 1.4:+79569936 None:intergenic
GTCCAAGGAAACAAATTCAT+TGG 0.347689 1.4:+79569958 None:intergenic
TAGAATTGGGAGTCTTATAC+TGG 0.362640 1.4:-79569844 MS.gene070955:CDS
TGAAGAGATTCGTTACTGTT+TGG 0.363134 1.4:-79568996 MS.gene070955:CDS
ACGATCGCTGGATTCGCATT+TGG 0.379921 1.4:-79568940 MS.gene070955:CDS
TCCAAGGAAACAAATTCATT+GGG 0.393021 1.4:+79569959 None:intergenic
TACCATGTAATTGTATCCTC+TGG 0.413679 1.4:+79569766 None:intergenic
TTAATGTATTCCTTTCTAGC+AGG 0.416178 1.4:+79568597 None:intergenic
CTGGATTCGCATTTGGATCA+AGG 0.420712 1.4:-79568933 MS.gene070955:CDS
CAGTTAATGAGGTTGTTTAT+GGG 0.429331 1.4:-79568663 MS.gene070955:CDS
GCTAGAAAGGAATACATTAA+GGG 0.444455 1.4:-79568594 MS.gene070955:CDS
GGAAGATAACCCTGCTAGAA+AGG 0.448092 1.4:-79568607 MS.gene070955:CDS
GTTTCCTTGGACCAATTAGC+AGG 0.487915 1.4:-79569947 MS.gene070955:intron
CTACACTGAGCACATTCATC+AGG 0.491343 1.4:-79569020 MS.gene070955:CDS
CGTTACTGTTTGGAGGGAAG+TGG 0.493451 1.4:-79568986 MS.gene070955:CDS
AAGGGCTTGACTGAGAAAAC+TGG 0.495479 1.4:-79568576 MS.gene070955:CDS
TAGCCATGGTGAGGAAGTCT+TGG 0.507430 1.4:+79569983 None:intergenic
AGAGATTCGTTACTGTTTGG+AGG 0.508489 1.4:-79568993 MS.gene070955:CDS
TAATGTATTCCTTTCTAGCA+GGG 0.526328 1.4:+79568598 None:intergenic
ATTCGCATTTGGATCAAGGC+TGG 0.542481 1.4:-79568929 MS.gene070955:CDS
AACCGATGGTAGATTCCTGC+TGG 0.548643 1.4:+79568890 None:intergenic
GGATTTACTTGATTTATGCC+CGG 0.551505 1.4:-79569080 MS.gene070955:CDS
CAAATTCATTGGGGTAGCCA+TGG 0.553222 1.4:+79569969 None:intergenic
GAGATTCGTTACTGTTTGGA+GGG 0.561571 1.4:-79568992 MS.gene070955:CDS
TACCAGCAGGAATCTACCAT+CGG 0.565483 1.4:-79568892 MS.gene070955:CDS
GAGGTTGTTTATGGGTGAGC+CGG 0.570174 1.4:-79568655 MS.gene070955:CDS
CTAGCAGGGTTATCTTCCTG+TGG 0.575444 1.4:+79568612 None:intergenic
CGATTATATGCTGTCCACAC+CGG 0.591841 1.4:+79568636 None:intergenic
CGACACAAATAACTATGTGA+AGG 0.598188 1.4:-79568861 MS.gene070955:intron
ATAACCTGCTAATTGGTCCA+AGG 0.599536 1.4:+79569943 None:intergenic
TTGTGTCGAGAGTGAACCGA+TGG 0.605251 1.4:+79568876 None:intergenic
TGTTTATGGGTGAGCCGGTG+TGG 0.615047 1.4:-79568650 MS.gene070955:CDS
GGGTCCTAATGAGCTGCAAG+TGG 0.616526 1.4:+79568549 None:intergenic
AGCCAGAGGATACAATTACA+TGG 0.637470 1.4:-79569768 MS.gene070955:intron
GACAGCATATAATCGACCAC+AGG 0.646228 1.4:-79568628 MS.gene070955:CDS
TCATTGGGGTAGCCATGGTG+AGG 0.672207 1.4:+79569974 None:intergenic
GATCCAAGACTTCCTCACCA+TGG 0.679886 1.4:-79569986 MS.gene070955:CDS
CCAAGGAAACAAATTCATTG+GGG 0.686962 1.4:+79569960 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCTAATATATTGTAAA+CGG - chr1.4:79568988-79569007 MS.gene070955:CDS 10.0%
!! AATTAGTTGAAAATAACTTA+CGG - chr1.4:79569202-79569221 MS.gene070955:intron 15.0%
!!! AATTGTAATTTTTGTAGAAT+TGG - chr1.4:79568700-79568719 MS.gene070955:intron 15.0%
!!! ATTGTAATTTTTGTAGAATT+GGG - chr1.4:79568701-79568720 MS.gene070955:intron 15.0%
!! ACATGTTATAAGATTAAAAC+AGG - chr1.4:79568950-79568969 MS.gene070955:CDS 20.0%
!! TTACATCAACAAAAATTTGA+AGG + chr1.4:79569014-79569033 None:intergenic 20.0%
!! TTTGTTGATGTAAATTTCTT+AGG - chr1.4:79569021-79569040 MS.gene070955:CDS 20.0%
!!! ATTTTGATTGTACTAATTGA+AGG - chr1.4:79569374-79569393 MS.gene070955:intron 20.0%
!!! TCATAATTTTCAACTTTTTC+CGG + chr1.4:79569500-79569519 None:intergenic 20.0%
!!! TTTTGATTGTACTAATTGAA+GGG - chr1.4:79569375-79569394 MS.gene070955:intron 20.0%
! ACCTATAAATTTCTTGATCA+AGG - chr1.4:79569726-79569745 MS.gene070955:intron 25.0%
! ATAGCTTATGAAAACAACTT+TGG + chr1.4:79569233-79569252 None:intergenic 25.0%
! ATGATTAATAACCTGCTAAT+TGG + chr1.4:79568625-79568644 None:intergenic 25.0%
! ATTTCATCTTTCAAATGTTC+TGG - chr1.4:79569400-79569419 MS.gene070955:intron 25.0%
! GTACTATTTGAATATGTTGT+AGG - chr1.4:79569457-79569476 MS.gene070955:intron 25.0%
! TTGAAATTGATCAAGATTCA+AGG - chr1.4:79569812-79569831 MS.gene070955:CDS 25.0%
! TTGATCAAGAAATTTATAGG+TGG + chr1.4:79569727-79569746 None:intergenic 25.0%
! TTTATTGTGACACAAGTTTA+AGG - chr1.4:79568674-79568693 MS.gene070955:intron 25.0%
! TTTCATCTTTCAAATGTTCT+GGG - chr1.4:79569401-79569420 MS.gene070955:intron 25.0%
!! AAGTTATCTTTGAGAGTTTA+TGG - chr1.4:79569178-79569197 MS.gene070955:intron 25.0%
!! TTTCTAGTGATACAATTACA+TGG - chr1.4:79568877-79568896 MS.gene070955:CDS 25.0%
!!! CATAATTTTCAACTTTTTCC+GGG + chr1.4:79569499-79569518 None:intergenic 25.0%
ATAAGTTGTTTACCTAACAG+TGG + chr1.4:79569075-79569094 None:intergenic 30.0%
CAGTTAATGAGGTTGTTTAT+GGG - chr1.4:79569895-79569914 MS.gene070955:intron 30.0%
GAGCTCAAATAAGTCAATTT+AGG - chr1.4:79569288-79569307 MS.gene070955:intron 30.0%
GATCATTCTCATAGTCATTA+GGG + chr1.4:79568748-79568767 None:intergenic 30.0%
GCCTTGATCAAGAAATTTAT+AGG + chr1.4:79569730-79569749 None:intergenic 30.0%
GCTAGAAAGGAATACATTAA+GGG - chr1.4:79569964-79569983 MS.gene070955:CDS 30.0%
GTACAATCAAAATGTTCAAC+AGG + chr1.4:79569367-79569386 None:intergenic 30.0%
TCCAAGGAAACAAATTCATT+GGG + chr1.4:79568602-79568621 None:intergenic 30.0%
TGATCATTCTCATAGTCATT+AGG + chr1.4:79568749-79568768 None:intergenic 30.0%
TGCTAGAAAGGAATACATTA+AGG - chr1.4:79569963-79569982 MS.gene070955:CDS 30.0%
TTACATGGTAAGATTGAAAG+TGG - chr1.4:79568892-79568911 MS.gene070955:CDS 30.0%
! TAATGTATTCCTTTCTAGCA+GGG + chr1.4:79569963-79569982 None:intergenic 30.0%
! TTAATGTATTCCTTTCTAGC+AGG + chr1.4:79569964-79569983 None:intergenic 30.0%
!! TTGATCAAGGCTTCATTTTT+GGG - chr1.4:79569739-79569758 MS.gene070955:intron 30.0%
!!! GAAAGTGGATTTTTTCTTGT+GGG - chr1.4:79568907-79568926 MS.gene070955:CDS 30.0%
!!! TGAAAGTGGATTTTTTCTTG+TGG - chr1.4:79568906-79568925 MS.gene070955:CDS 30.0%
AAAAACATTAGAGAAGCCAG+AGG - chr1.4:79568776-79568795 MS.gene070955:intron 35.0%
ACTTATTTGAGCTCATCTAC+CGG + chr1.4:79569282-79569301 None:intergenic 35.0%
CAAGATTCAAGGTGATATGT+TGG - chr1.4:79569823-79569842 MS.gene070955:CDS 35.0%
CCAAGGAAACAAATTCATTG+GGG + chr1.4:79568601-79568620 None:intergenic 35.0%
CGACACAAATAACTATGTGA+AGG - chr1.4:79569697-79569716 MS.gene070955:intron 35.0%
GCAGTTAATGAGGTTGTTTA+TGG - chr1.4:79569894-79569913 MS.gene070955:intron 35.0%
GGATTTACTTGATTTATGCC+CGG - chr1.4:79569478-79569497 MS.gene070955:intron 35.0%
GTCCAAGGAAACAAATTCAT+TGG + chr1.4:79568603-79568622 None:intergenic 35.0%
TAAGTATTTGCGAGACTGTT+CGG + chr1.4:79569257-79569276 None:intergenic 35.0%
TACCATGTAATTGTATCCTC+TGG + chr1.4:79568795-79568814 None:intergenic 35.0%
TGAAGAGATTCGTTACTGTT+TGG - chr1.4:79569562-79569581 MS.gene070955:intron 35.0%
TTGTTCGATGCATATGTATG+TGG - chr1.4:79569865-79569884 MS.gene070955:intron 35.0%
! CTTGATCAAGGCTTCATTTT+TGG - chr1.4:79569738-79569757 MS.gene070955:intron 35.0%
! GATCTTATCATTTTACCAGC+AGG - chr1.4:79569653-79569672 MS.gene070955:intron 35.0%
! TAGAATTGGGAGTCTTATAC+TGG - chr1.4:79568714-79568733 MS.gene070955:intron 35.0%
AGAGATTCGTTACTGTTTGG+AGG - chr1.4:79569565-79569584 MS.gene070955:intron 40.0%
AGCCAGAGGATACAATTACA+TGG - chr1.4:79568790-79568809 MS.gene070955:intron 40.0%
AGTCTCGCAAATACTTATGC+CGG - chr1.4:79569260-79569279 MS.gene070955:intron 40.0%
ATAACCTGCTAATTGGTCCA+AGG + chr1.4:79568618-79568637 None:intergenic 40.0%
GAGATTCGTTACTGTTTGGA+GGG - chr1.4:79569566-79569585 MS.gene070955:intron 40.0%
! CCCCAATGAATTTGTTTCCT+TGG - chr1.4:79568598-79568617 MS.gene070955:CDS 40.0%
!!! AAGGCTTCATTTTTGGGATG+TGG - chr1.4:79569745-79569764 MS.gene070955:intron 40.0%
!!! GTGGTTTTGTGCAGTTAATG+AGG - chr1.4:79569884-79569903 MS.gene070955:intron 40.0%
AAGGGCTTGACTGAGAAAAC+TGG - chr1.4:79569982-79570001 MS.gene070955:CDS 45.0%
ATTCGCATTTGGATCAAGGC+TGG - chr1.4:79569629-79569648 MS.gene070955:intron 45.0%
CAAATTCATTGGGGTAGCCA+TGG + chr1.4:79568592-79568611 None:intergenic 45.0%
CGATTATATGCTGTCCACAC+CGG + chr1.4:79569925-79569944 None:intergenic 45.0%
CTACACTGAGCACATTCATC+AGG - chr1.4:79569538-79569557 MS.gene070955:intron 45.0%
CTGGATTCGCATTTGGATCA+AGG - chr1.4:79569625-79569644 MS.gene070955:intron 45.0%
GACAGCATATAATCGACCAC+AGG - chr1.4:79569930-79569949 MS.gene070955:intron 45.0%
GGAAGATAACCCTGCTAGAA+AGG - chr1.4:79569951-79569970 MS.gene070955:CDS 45.0%
GTTTCCTTGGACCAATTAGC+AGG - chr1.4:79568611-79568630 MS.gene070955:CDS 45.0%
TACCAGCAGGAATCTACCAT+CGG - chr1.4:79569666-79569685 MS.gene070955:intron 45.0%
TTCGAGACAAAAACGATCGC+TGG - chr1.4:79569606-79569625 MS.gene070955:intron 45.0%
AACCGATGGTAGATTCCTGC+TGG + chr1.4:79569671-79569690 None:intergenic 50.0%
ACGATCGCTGGATTCGCATT+TGG - chr1.4:79569618-79569637 MS.gene070955:intron 50.0%
CGTTACTGTTTGGAGGGAAG+TGG - chr1.4:79569572-79569591 MS.gene070955:intron 50.0%
CTAGCAGGGTTATCTTCCTG+TGG + chr1.4:79569949-79569968 None:intergenic 50.0%
GAGGTTGTTTATGGGTGAGC+CGG - chr1.4:79569903-79569922 MS.gene070955:intron 50.0%
GATCCAAGACTTCCTCACCA+TGG - chr1.4:79568572-79568591 MS.gene070955:CDS 50.0%
TAGCCATGGTGAGGAAGTCT+TGG + chr1.4:79568578-79568597 None:intergenic 50.0%
TTAGCAGCAACGCCACTGTT+AGG - chr1.4:79569060-79569079 MS.gene070955:CDS 50.0%
TTGTGTCGAGAGTGAACCGA+TGG + chr1.4:79569685-79569704 None:intergenic 50.0%
TCATTGGGGTAGCCATGGTG+AGG + chr1.4:79568587-79568606 None:intergenic 55.0%
TGTTTATGGGTGAGCCGGTG+TGG - chr1.4:79569908-79569927 MS.gene070955:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 79568554 79570026 79568554 ID=MS.gene070955
chr1.4 mRNA 79568554 79570026 79568554 ID=MS.gene070955.t1;Parent=MS.gene070955
chr1.4 exon 79569948 79570026 79569948 ID=MS.gene070955.t1.exon1;Parent=MS.gene070955.t1
chr1.4 CDS 79569948 79570026 79569948 ID=cds.MS.gene070955.t1;Parent=MS.gene070955.t1
chr1.4 exon 79569769 79569863 79569769 ID=MS.gene070955.t1.exon2;Parent=MS.gene070955.t1
chr1.4 CDS 79569769 79569863 79569769 ID=cds.MS.gene070955.t1;Parent=MS.gene070955.t1
chr1.4 exon 79568862 79569101 79568862 ID=MS.gene070955.t1.exon3;Parent=MS.gene070955.t1
chr1.4 CDS 79568862 79569101 79568862 ID=cds.MS.gene070955.t1;Parent=MS.gene070955.t1
chr1.4 exon 79568554 79568682 79568554 ID=MS.gene070955.t1.exon4;Parent=MS.gene070955.t1
chr1.4 CDS 79568554 79568682 79568554 ID=cds.MS.gene070955.t1;Parent=MS.gene070955.t1
Gene Sequence

>MS.gene070955

ATGGACAATAGCAATGAAGATCCAAGACTTCCTCACCATGGCTACCCCAATGAATTTGTTTCCTTGGACCAATTAGCAGGTTATTAATCATCTTTCTGTCTATGTAATATATAGTTTTAATTTATTGTGACACAAGTTTAAGGAAAAATTGTAATTTTTGTAGAATTGGGAGTCTTATACTGGAAGCTGAACCCTAATGACTATGAGAATGATCAAGAATTGAAAAACATTAGAGAAGCCAGAGGATACAATTACATGGTAATAATATTGTTGTCTTTTTTATTTTTATTTTTTTTTATTAAATGCAATTATTCATATTCAGTTTTCTAGTGATACAATTACATGGTAAGATTGAAAGTGGATTTTTTCTTGTGGGTTTAACATGTAAAAGAAAAAACATGTTATAAGATTAAAACAGGAACATATGACTTACTATTATCTAATATATTGTAAACGGCCTTCAAATTTTTGTTGATGTAAATTTCTTAGGACATGCTGCACAGAGCTTAGCAGCAACGCCACTGTTAGGTAAACAACTTATTAAGTGTTTGTCTATAAACTATTTTTATAACAAAAGATAAAATTAAGTCAATTATTTATATATAAGTTGTAAGTTGTATTCATAAGTTATCTTTGAGAGTTTATGGAAATTAGTTGAAAATAACTTACGGAGATGCCAAAGTTGTTTTCATAAGCTATACCGAACAGTCTCGCAAATACTTATGCCGGTAGATGAGCTCAAATAAGTCAATTTAGGAACACTTGCACTAGAGTTGTTAGAGATATGACGATTTCAAGATTAATTTTTGACCTGTTGAACATTTTGATTGTACTAATTGAAGGGAAATTTCATCTTTCAAATGTTCTGGGTGTTGATAACATCTTCGTATCTTGAATATTTCTGTACTATTTGAATATGTTGTAGGATTTACTTGATTTATGCCCGGAAAAAGTTGAAAATTATGAAGAGAAGTTGAAGAACTTCTACACTGAGCACATTCATCAGGATGAAGAGATTCGTTACTGTTTGGAGGGAAGTGGCTACTTCGATGTTCGAGACAAAAACGATCGCTGGATTCGCATTTGGATCAAGGCTGGTGATCTTATCATTTTACCAGCAGGAATCTACCATCGGTTCACTCTCGACACAAATAACTATGTGAAGGTAATCCACCTATAAATTTCTTGATCAAGGCTTCATTTTTGGGATGTGGAATGTAGAAAGTAATGCAGTTTTAACTTTAAGCATGTTTAAAACTTGAAATTGATCAAGATTCAAGGTGATATGTTGGATGATTAGAATTAATTGTTTTGTTCGATGCATATGTATGTGGTTTTGTGCAGTTAATGAGGTTGTTTATGGGTGAGCCGGTGTGGACAGCATATAATCGACCACAGGAAGATAACCCTGCTAGAAAGGAATACATTAAGGGCTTGACTGAGAAAACTGGTGTTCCACTTGCAGCTCATTAG

Protein sequence

>MS.gene070955.t1

MDNSNEDPRLPHHGYPNEFVSLDQLAELGVLYWKLNPNDYENDQELKNIREARGYNYMDLLDLCPEKVENYEEKLKNFYTEHIHQDEEIRYCLEGSGYFDVRDKNDRWIRIWIKAGDLIILPAGIYHRFTLDTNNYVKLMRLFMGEPVWTAYNRPQEDNPARKEYIKGLTEKTGVPLAAH