AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24476


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24476.t1 MTR_1g018030 94.330 194 9 2 2 195 1 192 1.61e-134 374
MS.gene24476.t1 MTR_7g073990 42.282 149 75 2 47 187 97 242 4.56e-31 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24476.t1 AT1G26220 60.591 203 65 4 2 195 1 197 9.18e-81 239
MS.gene24476.t1 AT1G32070 41.985 131 60 1 76 190 127 257 9.06e-29 108
MS.gene24476.t1 AT1G32070 41.667 132 60 1 76 190 126 257 2.82e-28 106
MS.gene24476.t1 AT1G32070 41.667 132 60 1 76 190 127 258 2.88e-28 106

Find 50 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTTTGTTAAGTGCTTCT+AGG 0.216082 1.1:+4636021 None:intergenic
TACTCTGAGCCAAGAGTTCT+TGG 0.233326 1.1:-4635735 MS.gene24476:CDS
GATCCTTCGTTTCAAGGGCT+TGG 0.286511 1.1:-4635828 MS.gene24476:CDS
AACAACAACATCCCATATTA+TGG 0.310068 1.1:+4635851 None:intergenic
GTTGTTGATCCTTCGTTTCA+AGG 0.353170 1.1:-4635834 MS.gene24476:CDS
CTCAGAGTAAAGAGCAATAT+TGG 0.361910 1.1:+4635749 None:intergenic
ATCCTTCGTTTCAAGGGCTT+GGG 0.363113 1.1:-4635827 MS.gene24476:CDS
TCGTTTCAAGGGCTTGGGCT+AGG 0.372956 1.1:-4635822 MS.gene24476:CDS
CTGTTGTGGAGAGGCTTATG+AGG 0.408843 1.1:-4635794 MS.gene24476:CDS
AAGTTAAGTCGTAGGGAGGA+TGG 0.419412 1.1:+4636179 None:intergenic
CGATAAAAGCCAAGAACTCT+TGG 0.431331 1.1:+4635726 None:intergenic
TAGGCTTGCTTGACAAGAAA+TGG 0.442809 1.1:+4636121 None:intergenic
CGTTTCAAGGGCTTGGGCTA+GGG 0.454257 1.1:-4635821 MS.gene24476:CDS
TTGTTGATCCTTCGTTTCAA+GGG 0.483590 1.1:-4635833 MS.gene24476:CDS
GTTGCATTTGCAAGAGCTAC+TGG 0.489343 1.1:-4635891 MS.gene24476:CDS
TTAACCCCTCTGAGGTTCTC+CGG 0.494474 1.1:+4636045 None:intergenic
TTTGAAGAAGTTAAGTCGTA+GGG 0.496530 1.1:+4636172 None:intergenic
CAAGAAATGGTGAGGTTTCG+TGG 0.507526 1.1:+4636134 None:intergenic
TGAGGGATCTTGTAGGAAGA+GGG 0.520334 1.1:-4635776 MS.gene24476:CDS
ACTCGAGCACACTGACTCAA+TGG 0.522170 1.1:-4635949 MS.gene24476:CDS
ATTTGAAGAAGTTAAGTCGT+AGG 0.522993 1.1:+4636171 None:intergenic
TGGGCTAGGGAAAGCTGTTG+TGG 0.530219 1.1:-4635808 MS.gene24476:CDS
ATGAGGGATCTTGTAGGAAG+AGG 0.534453 1.1:-4635777 MS.gene24476:CDS
AGTCGTAGGGAGGATGGAAG+AGG 0.540218 1.1:+4636185 None:intergenic
ATATTGAGAGTGGGTAGTCT+AGG 0.541441 1.1:+4636102 None:intergenic
ACCATTCCACGAATACCATC+AGG 0.547694 1.1:+4635681 None:intergenic
AGGCTTATGAGGGATCTTGT+AGG 0.549706 1.1:-4635783 MS.gene24476:CDS
AGCACACTGACTCAATGGTA+TGG 0.558828 1.1:-4635944 MS.gene24476:CDS
ACAAAGTATTCGTAGCAGTA+GGG 0.561588 1.1:-4636007 MS.gene24476:CDS
TCCTGATGGTATTCGTGGAA+TGG 0.563938 1.1:-4635682 MS.gene24476:CDS
AACAAAGTATTCGTAGCAGT+AGG 0.566118 1.1:-4636008 MS.gene24476:CDS
GAAGAAGTTAAGTCGTAGGG+AGG 0.570827 1.1:+4636175 None:intergenic
GCTGATCCTGATGGTATTCG+TGG 0.578470 1.1:-4635687 MS.gene24476:CDS
AGCCCAAGCCCTTGAAACGA+AGG 0.590595 1.1:+4635825 None:intergenic
GTTTCGTCGGATATTGAGAG+TGG 0.590666 1.1:+4636092 None:intergenic
ATTCTCCGGAGAACCTCAGA+GGG 0.594468 1.1:-4636050 MS.gene24476:CDS
TTGCTTGACAAGAAATGGTG+AGG 0.596575 1.1:+4636126 None:intergenic
TTCTAGGTTTAACCCCTCTG+AGG 0.598354 1.1:+4636037 None:intergenic
GATAATATGCTGCTCTTACG+TGG 0.618724 1.1:-4636221 None:intergenic
TGTTGTGGAGAGGCTTATGA+GGG 0.625783 1.1:-4635793 MS.gene24476:CDS
TAGGGAAAGCTGTTGTGGAG+AGG 0.629346 1.1:-4635803 MS.gene24476:CDS
AAATGGTGAGGTTTCGTGGT+GGG 0.635153 1.1:+4636138 None:intergenic
TTTGCAAGAGCTACTGGTGA+TGG 0.636078 1.1:-4635885 MS.gene24476:CDS
TTTCGTCGGATATTGAGAGT+GGG 0.646235 1.1:+4636093 None:intergenic
CATTCTCCGGAGAACCTCAG+AGG 0.650384 1.1:-4636051 MS.gene24476:CDS
TCGTAGCAGTAGGGTTTCCG+CGG 0.663293 1.1:-4635998 MS.gene24476:CDS
GGATCAGCAACAAAACCCAA+AGG 0.674227 1.1:+4635702 None:intergenic
GAAGAAGTCGCAGAATAATG+CGG 0.693298 1.1:-4635913 MS.gene24476:CDS
GAAATGGTGAGGTTTCGTGG+TGG 0.768689 1.1:+4636137 None:intergenic
TTCTCCGGAGAACCTCAGAG+GGG 0.790280 1.1:-4636049 MS.gene24476:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTTCAATGCCATAATAT+GGG - chr1.1:4636003-4636022 MS.gene24476:CDS 25.0%
AACAACAACATCCCATATTA+TGG + chr1.1:4636017-4636036 None:intergenic 30.0%
ATACTTTGTTAAGTGCTTCT+AGG + chr1.1:4635847-4635866 None:intergenic 30.0%
ATTTGAAGAAGTTAAGTCGT+AGG + chr1.1:4635697-4635716 None:intergenic 30.0%
TTTGAAGAAGTTAAGTCGTA+GGG + chr1.1:4635696-4635715 None:intergenic 30.0%
! GTGTTTTCAATGCCATAATA+TGG - chr1.1:4636002-4636021 MS.gene24476:CDS 30.0%
AAAAACGAGGTTTCATTCTC+CGG - chr1.1:4635801-4635820 MS.gene24476:CDS 35.0%
CTCAGAGTAAAGAGCAATAT+TGG + chr1.1:4636119-4636138 None:intergenic 35.0%
TTGTTGATCCTTCGTTTCAA+GGG - chr1.1:4636032-4636051 MS.gene24476:CDS 35.0%
! AACAAAGTATTCGTAGCAGT+AGG - chr1.1:4635857-4635876 MS.gene24476:CDS 35.0%
! ACAAAGTATTCGTAGCAGTA+GGG - chr1.1:4635858-4635877 MS.gene24476:CDS 35.0%
!! TCGTTTTTCTAGTGTTTCGT+CGG + chr1.1:4635789-4635808 None:intergenic 35.0%
ATATTGAGAGTGGGTAGTCT+AGG + chr1.1:4635766-4635785 None:intergenic 40.0%
CGATAAAAGCCAAGAACTCT+TGG + chr1.1:4636142-4636161 None:intergenic 40.0%
GAAGAAGTCGCAGAATAATG+CGG - chr1.1:4635952-4635971 MS.gene24476:CDS 40.0%
GACGAAACACTAGAAAAACG+AGG - chr1.1:4635788-4635807 MS.gene24476:CDS 40.0%
GTTGTTGATCCTTCGTTTCA+AGG - chr1.1:4636031-4636050 MS.gene24476:CDS 40.0%
TTGCTTGACAAGAAATGGTG+AGG + chr1.1:4635742-4635761 None:intergenic 40.0%
TTTCGTCGGATATTGAGAGT+GGG + chr1.1:4635775-4635794 None:intergenic 40.0%
! AGTGCAATGCGTATTTTCTC+AGG + chr1.1:4635899-4635918 None:intergenic 40.0%
! GTGCAATGCGTATTTTCTCA+GGG + chr1.1:4635898-4635917 None:intergenic 40.0%
! TAGGCTTGCTTGACAAGAAA+TGG + chr1.1:4635747-4635766 None:intergenic 40.0%
!! CTTGGCTTTTATCGACCTTT+GGG - chr1.1:4636148-4636167 MS.gene24476:CDS 40.0%
!! TCTTGGCTTTTATCGACCTT+TGG - chr1.1:4636147-4636166 MS.gene24476:CDS 40.0%
AAATGGTGAGGTTTCGTGGT+GGG + chr1.1:4635730-4635749 None:intergenic 45.0%
AAGTTAAGTCGTAGGGAGGA+TGG + chr1.1:4635689-4635708 None:intergenic 45.0%
ACCATTCCACGAATACCATC+AGG + chr1.1:4636187-4636206 None:intergenic 45.0%
AGGCTTATGAGGGATCTTGT+AGG - chr1.1:4636082-4636101 MS.gene24476:CDS 45.0%
ATCCTTCGTTTCAAGGGCTT+GGG - chr1.1:4636038-4636057 MS.gene24476:CDS 45.0%
ATGAGGGATCTTGTAGGAAG+AGG - chr1.1:4636088-4636107 MS.gene24476:CDS 45.0%
CAAGAAATGGTGAGGTTTCG+TGG + chr1.1:4635734-4635753 None:intergenic 45.0%
GAAGAAGTTAAGTCGTAGGG+AGG + chr1.1:4635693-4635712 None:intergenic 45.0%
GGATCAGCAACAAAACCCAA+AGG + chr1.1:4636166-4636185 None:intergenic 45.0%
GTTGCATTTGCAAGAGCTAC+TGG - chr1.1:4635974-4635993 MS.gene24476:CDS 45.0%
GTTTCGTCGGATATTGAGAG+TGG + chr1.1:4635776-4635795 None:intergenic 45.0%
TACTCTGAGCCAAGAGTTCT+TGG - chr1.1:4636130-4636149 MS.gene24476:CDS 45.0%
TCCTGATGGTATTCGTGGAA+TGG - chr1.1:4636183-4636202 MS.gene24476:CDS 45.0%
TGAGGGATCTTGTAGGAAGA+GGG - chr1.1:4636089-4636108 MS.gene24476:CDS 45.0%
TGTTGTGGAGAGGCTTATGA+GGG - chr1.1:4636072-4636091 MS.gene24476:CDS 45.0%
TTCTAGGTTTAACCCCTCTG+AGG + chr1.1:4635831-4635850 None:intergenic 45.0%
TTTGCAAGAGCTACTGGTGA+TGG - chr1.1:4635980-4635999 MS.gene24476:CDS 45.0%
!! AGCACACTGACTCAATGGTA+TGG - chr1.1:4635921-4635940 MS.gene24476:CDS 45.0%
!!! GGTTTTGTTGCTGATCCTGA+TGG - chr1.1:4636169-4636188 MS.gene24476:CDS 45.0%
ATTCTCCGGAGAACCTCAGA+GGG - chr1.1:4635815-4635834 MS.gene24476:CDS 50.0%
CTGTTGTGGAGAGGCTTATG+AGG - chr1.1:4636071-4636090 MS.gene24476:CDS 50.0%
GAAATGGTGAGGTTTCGTGG+TGG + chr1.1:4635731-4635750 None:intergenic 50.0%
GATCCTTCGTTTCAAGGGCT+TGG - chr1.1:4636037-4636056 MS.gene24476:CDS 50.0%
GCTGATCCTGATGGTATTCG+TGG - chr1.1:4636178-4636197 MS.gene24476:CDS 50.0%
TAGGGAAAGCTGTTGTGGAG+AGG - chr1.1:4636062-4636081 MS.gene24476:CDS 50.0%
!! ACTCGAGCACACTGACTCAA+TGG - chr1.1:4635916-4635935 MS.gene24476:CDS 50.0%
!! TTAACCCCTCTGAGGTTCTC+CGG + chr1.1:4635823-4635842 None:intergenic 50.0%
AGCCCAAGCCCTTGAAACGA+AGG + chr1.1:4636043-4636062 None:intergenic 55.0%
AGTCGTAGGGAGGATGGAAG+AGG + chr1.1:4635683-4635702 None:intergenic 55.0%
CATTCTCCGGAGAACCTCAG+AGG - chr1.1:4635814-4635833 MS.gene24476:CDS 55.0%
CGTTTCAAGGGCTTGGGCTA+GGG - chr1.1:4636044-4636063 MS.gene24476:CDS 55.0%
TCGTAGCAGTAGGGTTTCCG+CGG - chr1.1:4635867-4635886 MS.gene24476:CDS 55.0%
TCGTTTCAAGGGCTTGGGCT+AGG - chr1.1:4636043-4636062 MS.gene24476:CDS 55.0%
TTCTCCGGAGAACCTCAGAG+GGG - chr1.1:4635816-4635835 MS.gene24476:CDS 55.0%
! TGGGCTAGGGAAAGCTGTTG+TGG - chr1.1:4636057-4636076 MS.gene24476:CDS 55.0%
! ATTTTCTCAGGGTCGCGCCG+CGG + chr1.1:4635887-4635906 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 4635650 4636237 4635650 ID=MS.gene24476
chr1.1 mRNA 4635650 4636237 4635650 ID=MS.gene24476.t1;Parent=MS.gene24476
chr1.1 exon 4635650 4636237 4635650 ID=MS.gene24476.t1.exon1;Parent=MS.gene24476.t1
chr1.1 CDS 4635650 4636237 4635650 ID=cds.MS.gene24476.t1;Parent=MS.gene24476.t1
Gene Sequence

>MS.gene24476

ATGCTGCTCTTACGTGGCAACATCTATGCTCCTCTTCCATCCTCCCTACGACTTAACTTCTTCAAATCACAAACTCACCCACCACGAAACCTCACCATTTCTTGTCAAGCAAGCCTAGACTACCCACTCTCAATATCCGACGAAACACTAGAAAAACGAGGTTTCATTCTCCGGAGAACCTCAGAGGGGTTAAACCTAGAAGCACTTAACAAAGTATTCGTAGCAGTAGGGTTTCCGCGGCGCGACCCTGAGAAAATACGCATTGCACTCGAGCACACTGACTCAATGGTATGGATACAACAGAAGAAGTCGCAGAATAATGCGGTTGCATTTGCAAGAGCTACTGGTGATGGTGTTTTCAATGCCATAATATGGGATGTTGTTGTTGATCCTTCGTTTCAAGGGCTTGGGCTAGGGAAAGCTGTTGTGGAGAGGCTTATGAGGGATCTTGTAGGAAGAGGGATTACCAATATTGCTCTTTACTCTGAGCCAAGAGTTCTTGGCTTTTATCGACCTTTGGGTTTTGTTGCTGATCCTGATGGTATTCGTGGAATGGTTTACTCGACAAAAAACAAAAGAAAAAAATAG

Protein sequence

>MS.gene24476.t1

MLLLRGNIYAPLPSSLRLNFFKSQTHPPRNLTISCQASLDYPLSISDETLEKRGFILRRTSEGLNLEALNKVFVAVGFPRRDPEKIRIALEHTDSMVWIQQKKSQNNAVAFARATGDGVFNAIIWDVVVDPSFQGLGLGKAVVERLMRDLVGRGITNIALYSEPRVLGFYRPLGFVADPDGIRGMVYSTKNKRKK