Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54168.t1 | XP_003615753.2 | 95.8 | 744 | 19 | 1 | 1 | 732 | 1 | 744 | 0.00E+00 | 1406.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54168.t1 | Q9MB58 | 69.6 | 730 | 192 | 9 | 25 | 732 | 23 | 744 | 5.0e-280 | 964.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54168.t1 | G7K732 | 95.8 | 744 | 19 | 1 | 1 | 732 | 1 | 744 | 0.0e+00 | 1406.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene54168 | 0.80025 | 1.57E-48 | -1.69E-46 |
| MS.gene049217 | MS.gene54168 | 0.802596 | 5.21E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene54168 | 0.826049 | 3.36E-54 | -1.69E-46 |
| MS.gene049369 | MS.gene54168 | 0.808906 | 2.46E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene54168 | 0.88365 | 3.75E-71 | -1.69E-46 |
| MS.gene049491 | MS.gene54168 | 0.815764 | 7.82E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene54168 | 0.804328 | 2.28E-49 | -1.69E-46 |
| MS.gene049614 | MS.gene54168 | 0.82393 | 1.06E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene54168 | 0.858214 | 9.61E-63 | -1.69E-46 |
| MS.gene049662 | MS.gene54168 | 0.846015 | 2.83E-59 | -1.69E-46 |
| MS.gene049805 | MS.gene54168 | 0.81919 | 1.32E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene54168 | 0.848433 | 6.15E-60 | -1.69E-46 |
| MS.gene050163 | MS.gene54168 | 0.800863 | 1.18E-48 | -1.69E-46 |
| MS.gene050301 | MS.gene54168 | 0.809712 | 1.65E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene54168 | 0.819496 | 1.13E-52 | -1.69E-46 |
| MS.gene050432 | MS.gene54168 | 0.879428 | 1.26E-69 | -1.69E-46 |
| MS.gene050767 | MS.gene54168 | 0.845922 | 3.00E-59 | -1.69E-46 |
| MS.gene050770 | MS.gene54168 | 0.851298 | 9.72E-61 | -1.69E-46 |
| MS.gene050772 | MS.gene54168 | 0.812963 | 3.25E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene54168 | 0.838098 | 3.52E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene54168 | 0.833035 | 6.72E-56 | -1.69E-46 |
| MS.gene051228 | MS.gene54168 | 0.812419 | 4.28E-51 | -1.69E-46 |
| MS.gene051471 | MS.gene54168 | 0.819109 | 1.38E-52 | -1.69E-46 |
| MS.gene051692 | MS.gene54168 | 0.809426 | 1.90E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene54168 | 0.840559 | 8.08E-58 | -1.69E-46 |
| MS.gene052078 | MS.gene54168 | 0.819843 | 9.39E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene54168 | 0.826952 | 2.05E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene54168 | 0.808798 | 2.59E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene54168 | 0.81681 | 4.56E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene54168 | 0.83058 | 2.71E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene54168 | 0.852006 | 6.12E-61 | -1.69E-46 |
| MS.gene052322 | MS.gene54168 | 0.82138 | 4.16E-53 | -1.69E-46 |
| MS.gene052326 | MS.gene54168 | 0.830724 | 2.50E-55 | -1.69E-46 |
| MS.gene052340 | MS.gene54168 | 0.801389 | 9.22E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene54168 | 0.836502 | 9.01E-57 | -1.69E-46 |
| MS.gene052421 | MS.gene54168 | 0.811229 | 7.77E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene54168 | 0.811865 | 5.65E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene54168 | 0.831684 | 1.45E-55 | -1.69E-46 |
| MS.gene052485 | MS.gene54168 | 0.82908 | 6.29E-55 | -1.69E-46 |
| MS.gene052615 | MS.gene54168 | 0.836062 | 1.17E-56 | -1.69E-46 |
| MS.gene053009 | MS.gene54168 | 0.81121 | 7.85E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene54168 | 0.802916 | 4.47E-49 | -1.69E-46 |
| MS.gene053104 | MS.gene54168 | 0.816461 | 5.47E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene54168 | 0.821419 | 4.08E-53 | -1.69E-46 |
| MS.gene053209 | MS.gene54168 | 0.835096 | 2.05E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene54168 | 0.810798 | 9.64E-51 | -1.69E-46 |
| MS.gene053286 | MS.gene54168 | 0.80319 | 3.92E-49 | -1.69E-46 |
| MS.gene053322 | MS.gene54168 | 0.843388 | 1.45E-58 | -1.69E-46 |
| MS.gene053632 | MS.gene54168 | 0.811476 | 6.87E-51 | -1.69E-46 |
| MS.gene053758 | MS.gene54168 | 0.806545 | 7.81E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene54168 | 0.828711 | 7.72E-55 | -1.69E-46 |
| MS.gene054376 | MS.gene54168 | 0.84566 | 3.54E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene54168 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene054802 | MS.gene54168 | 0.83473 | 2.53E-56 | -1.69E-46 |
| MS.gene054803 | MS.gene54168 | 0.837449 | 5.16E-57 | -1.69E-46 |
| MS.gene054804 | MS.gene54168 | 0.839414 | 1.61E-57 | -1.69E-46 |
| MS.gene05505 | MS.gene54168 | 0.820237 | 7.63E-53 | -1.69E-46 |
| MS.gene055116 | MS.gene54168 | 0.813136 | 2.98E-51 | -1.69E-46 |
| MS.gene055254 | MS.gene54168 | 0.824609 | 7.36E-54 | -1.69E-46 |
| MS.gene055277 | MS.gene54168 | 0.851222 | 1.02E-60 | -1.69E-46 |
| MS.gene055390 | MS.gene54168 | 0.808218 | 3.45E-50 | -1.69E-46 |
| MS.gene055397 | MS.gene54168 | 0.872728 | 2.54E-67 | -1.69E-46 |
| MS.gene055412 | MS.gene54168 | 0.841502 | 4.57E-58 | -1.69E-46 |
| MS.gene055499 | MS.gene54168 | 0.87463 | 5.81E-68 | -1.69E-46 |
| MS.gene055647 | MS.gene54168 | 0.801094 | 1.06E-48 | -1.69E-46 |
| MS.gene055722 | MS.gene54168 | 0.802408 | 5.69E-49 | -1.69E-46 |
| MS.gene055896 | MS.gene54168 | 0.811583 | 6.51E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene54168 | 0.822628 | 2.14E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene54168 | 0.807619 | 4.63E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene54168 | 0.80289 | 4.53E-49 | -1.69E-46 |
| MS.gene056410 | MS.gene54168 | 0.801742 | 7.80E-49 | -1.69E-46 |
| MS.gene056609 | MS.gene54168 | 0.876572 | 1.26E-68 | -1.69E-46 |
| MS.gene056659 | MS.gene54168 | 0.823521 | 1.32E-53 | -1.69E-46 |
| MS.gene056897 | MS.gene54168 | 0.835458 | 1.66E-56 | -1.69E-46 |
| MS.gene057011 | MS.gene54168 | 0.846166 | 2.58E-59 | -1.69E-46 |
| MS.gene05702 | MS.gene54168 | 0.822643 | 2.12E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene54168 | 0.811436 | 7.01E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene54168 | 0.830988 | 2.15E-55 | -1.69E-46 |
| MS.gene057129 | MS.gene54168 | 0.859212 | 4.83E-63 | -1.69E-46 |
| MS.gene057146 | MS.gene54168 | 0.827753 | 1.31E-54 | -1.69E-46 |
| MS.gene05733 | MS.gene54168 | 0.820585 | 6.35E-53 | -1.69E-46 |
| MS.gene05735 | MS.gene54168 | 0.824006 | 1.02E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene54168 | 0.80809 | 3.67E-50 | -1.69E-46 |
| MS.gene057749 | MS.gene54168 | 0.802536 | 5.36E-49 | -1.69E-46 |
| MS.gene058006 | MS.gene54168 | 0.803869 | 2.84E-49 | -1.69E-46 |
| MS.gene058082 | MS.gene54168 | 0.805206 | 1.49E-49 | -1.69E-46 |
| MS.gene058130 | MS.gene54168 | 0.816185 | 6.30E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene54168 | 0.823379 | 1.43E-53 | -1.69E-46 |
| MS.gene058293 | MS.gene54168 | 0.818681 | 1.73E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene54168 | 0.832305 | 1.02E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene54168 | 0.87311 | 1.90E-67 | -1.69E-46 |
| MS.gene058723 | MS.gene54168 | 0.805894 | 1.07E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene54168 | 0.820817 | 5.61E-53 | -1.69E-46 |
| MS.gene059029 | MS.gene54168 | 0.82041 | 6.96E-53 | -1.69E-46 |
| MS.gene05934 | MS.gene54168 | 0.805233 | 1.47E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene54168 | 0.844357 | 7.95E-59 | -1.69E-46 |
| MS.gene059597 | MS.gene54168 | 0.811871 | 5.64E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene54168 | 0.824085 | 9.77E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene54168 | 0.854864 | 9.26E-62 | -1.69E-46 |
| MS.gene059692 | MS.gene54168 | 0.858357 | 8.71E-63 | -1.69E-46 |
| MS.gene059695 | MS.gene54168 | 0.856702 | 2.69E-62 | -1.69E-46 |
| MS.gene059708 | MS.gene54168 | 0.834039 | 3.77E-56 | -1.69E-46 |
| MS.gene059858 | MS.gene54168 | 0.801839 | 7.45E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene54168 | 0.817548 | 3.11E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene54168 | 0.856821 | 2.48E-62 | -1.69E-46 |
| MS.gene06037 | MS.gene54168 | 0.814228 | 1.71E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene54168 | 0.855677 | 5.37E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene54168 | 0.841905 | 3.58E-58 | -1.69E-46 |
| MS.gene061041 | MS.gene54168 | 0.840484 | 8.46E-58 | -1.69E-46 |
| MS.gene061049 | MS.gene54168 | 0.822232 | 2.64E-53 | -1.69E-46 |
| MS.gene061051 | MS.gene54168 | -0.822695 | 2.06E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene54168 | 0.845518 | 3.86E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54168.t1 | MTR_5g071900 | 95.833 | 744 | 19 | 1 | 1 | 732 | 1 | 744 | 0.0 | 1474 |
| MS.gene54168.t1 | MTR_3g464770 | 74.167 | 720 | 160 | 5 | 25 | 732 | 31 | 736 | 0.0 | 1048 |
| MS.gene54168.t1 | MTR_3g464770 | 73.889 | 720 | 160 | 6 | 25 | 732 | 31 | 734 | 0.0 | 1038 |
| MS.gene54168.t1 | MTR_3g464770 | 77.259 | 664 | 140 | 2 | 69 | 732 | 2 | 654 | 0.0 | 1028 |
| MS.gene54168.t1 | MTR_3g464770 | 69.550 | 578 | 150 | 5 | 25 | 590 | 31 | 594 | 0.0 | 764 |
| MS.gene54168.t1 | MTR_8g066450 | 81.132 | 159 | 29 | 1 | 366 | 523 | 1 | 159 | 1.68e-87 | 273 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54168.t1 | AT1G07110 | 69.589 | 730 | 192 | 9 | 25 | 732 | 23 | 744 | 0.0 | 988 |
| MS.gene54168.t1 | AT3G30200 | 64.103 | 78 | 26 | 1 | 132 | 209 | 50 | 125 | 3.80e-20 | 95.1 |
Find 174 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACAAAATCTTCTAGTTTA+AGG | 0.070887 | 5.4:-61904892 | MS.gene54168:CDS |
| GTTATTGCAGATTATCTTTC+TGG | 0.111386 | 5.4:-61901733 | MS.gene54168:intron |
| GCATGTTCTTCTTGATTTCT+TGG | 0.127562 | 5.4:+61896586 | None:intergenic |
| AGTTAAATATTTACGGTTTC+AGG | 0.168472 | 5.4:+61908114 | None:intergenic |
| AAGAATTGCCTTCTCATAAT+TGG | 0.211422 | 5.4:+61901509 | None:intergenic |
| TCCTCGTGGGGAGTCTTATT+TGG | 0.222816 | 5.4:-61896362 | MS.gene54168:CDS |
| CTACAAATAAACCAGTAAAA+AGG | 0.237459 | 5.4:+61910784 | None:intergenic |
| GAGATAGAGTTGGGAGTTAC+AGG | 0.246273 | 5.4:-61895675 | MS.gene54168:CDS |
| TGCATTGCTTGGGGATTGTT+TGG | 0.252838 | 5.4:-61910838 | MS.gene54168:CDS |
| CAAGGTGAATTGTCGTCTTT+TGG | 0.260445 | 5.4:+61909566 | None:intergenic |
| CCATGCCGAGTGAGTAATAT+TGG | 0.285665 | 5.4:+61898310 | None:intergenic |
| CGCTCAAGCTCAATAATTAC+AGG | 0.286406 | 5.4:+61896233 | None:intergenic |
| GAATGTCTCTTCTGCGAGTT+TGG | 0.290246 | 5.4:-61908295 | MS.gene54168:CDS |
| AGCTAGTTGGAGGGCTTATA+AGG | 0.303691 | 5.4:-61909396 | MS.gene54168:CDS |
| CAGAAGCTTGCCAAATTTGT+TGG | 0.309090 | 5.4:-61897400 | MS.gene54168:CDS |
| AAGAAATATGCTGATGAAAT+TGG | 0.310175 | 5.4:-61901871 | MS.gene54168:CDS |
| CGCCAAATGTTTATCCTCCT+TGG | 0.313778 | 5.4:+61904634 | None:intergenic |
| GCATATACAGCAGATGAATT+TGG | 0.316849 | 5.4:+61908228 | None:intergenic |
| TTTGTTCTTCTGCATTGCTT+GGG | 0.321000 | 5.4:-61910848 | MS.gene54168:CDS |
| GCAGCTGGAGCTGTTGCAGC+TGG | 0.326927 | 5.4:-61904683 | MS.gene54168:CDS |
| TTTGAGAATTTCAGAAGCTT+TGG | 0.329623 | 5.4:-61909609 | MS.gene54168:intron |
| ATTTACTGTTGATCTTAGTT+TGG | 0.338847 | 5.4:-61904867 | MS.gene54168:CDS |
| ACAGAAACATAGAGCAATTC+AGG | 0.344331 | 5.4:+61910924 | None:intergenic |
| TTACGTGCCTCTATGCCTTC+AGG | 0.365144 | 5.4:+61902755 | None:intergenic |
| CGTAGCCCAGCCTCAAAATC+TGG | 0.366497 | 5.4:+61901552 | None:intergenic |
| GCAACAGCTCCAGCTGCTGC+TGG | 0.372798 | 5.4:+61904689 | None:intergenic |
| CAAAGTTTCTTCCTTTGATC+TGG | 0.373266 | 5.4:-61909420 | MS.gene54168:CDS |
| CTGACAGCTGGCCCCATTGT+TGG | 0.374142 | 5.4:-61896761 | MS.gene54168:CDS |
| TGCCGTTGCTGATCAGATGC+TGG | 0.374741 | 5.4:-61904660 | MS.gene54168:CDS |
| TCGCTGCTTGCTACTGTTTG+TGG | 0.379212 | 5.4:+61901893 | None:intergenic |
| GTACACCCAGGTAGCAAAAC+TGG | 0.384927 | 5.4:-61902055 | MS.gene54168:intron |
| TTGGAGGAGATCCTGCATTA+AGG | 0.386451 | 5.4:-61898258 | MS.gene54168:intron |
| TCAATACCCTGGAGCCATCT+AGG | 0.388149 | 5.4:-61905030 | MS.gene54168:CDS |
| CCGAATCGTGTACTCGTTGC+GGG | 0.392031 | 5.4:-61909527 | MS.gene54168:CDS |
| TTTGAAAGAAATTCCACATA+TGG | 0.394607 | 5.4:-61896038 | MS.gene54168:CDS |
| CCTCAAGCACGGAGCTAGTC+AGG | 0.398596 | 5.4:-61903872 | MS.gene54168:intron |
| ACCTTAGGAAATCCAACAAT+GGG | 0.399597 | 5.4:+61896749 | None:intergenic |
| ATACCCACTATTCCGTGATC+TGG | 0.402871 | 5.4:+61908185 | None:intergenic |
| TAAGGAGAACTTTCATCCTT+CGG | 0.403360 | 5.4:-61909378 | MS.gene54168:CDS |
| GATAATGTGAGAGGCAGAAT+TGG | 0.406339 | 5.4:-61898277 | MS.gene54168:CDS |
| CCTTTGATCTGGCAGCTAGT+TGG | 0.408429 | 5.4:-61909409 | MS.gene54168:CDS |
| CCCCATTGTTGGATTTCCTA+AGG | 0.411262 | 5.4:-61896750 | MS.gene54168:intron |
| CGACATTCAGACACCCTAGA+TGG | 0.411807 | 5.4:+61905016 | None:intergenic |
| CTATTGCAGATCCCGCAAAA+AGG | 0.420982 | 5.4:-61896404 | MS.gene54168:intron |
| AATCCAATCCGCGCTATCGC+TGG | 0.428193 | 5.4:-61910891 | MS.gene54168:CDS |
| GCCAAATGTTTATCCTCCTT+GGG | 0.434723 | 5.4:+61904635 | None:intergenic |
| AGAGAAAAGATACAAACTAA+TGG | 0.435041 | 5.4:-61895646 | MS.gene54168:CDS |
| TTTGGAGAACTTGAGGGAGC+TGG | 0.436441 | 5.4:+61908246 | None:intergenic |
| GTTTAAGCTTGATAATGATC+AGG | 0.436859 | 5.4:-61909474 | MS.gene54168:CDS |
| TTGATCTGGCAGCTAGTTGG+AGG | 0.444690 | 5.4:-61909406 | MS.gene54168:CDS |
| TCCCAAGGAGGATAAACATT+TGG | 0.450552 | 5.4:-61904636 | MS.gene54168:CDS |
| AATTGTTTCAACAGTGAAGC+TGG | 0.453800 | 5.4:-61897436 | MS.gene54168:intron |
| AATTCATTTCCAGCAGCAGC+TGG | 0.462347 | 5.4:-61904698 | MS.gene54168:CDS |
| TGGTGAGATACCACAACAAC+AGG | 0.467671 | 5.4:+61896200 | None:intergenic |
| TCTGAATTTATGCTGACGTC+GGG | 0.470133 | 5.4:+61909341 | None:intergenic |
| CACTCGGCATGGAGAAAGTC+AGG | 0.470560 | 5.4:-61898299 | MS.gene54168:CDS |
| CATGCCGAGTGAGTAATATT+GGG | 0.473038 | 5.4:+61898311 | None:intergenic |
| AATCCCAGATCACGGAATAG+TGG | 0.473281 | 5.4:-61908189 | MS.gene54168:CDS |
| TTTCCAGCGATAGCGCGGAT+TGG | 0.477364 | 5.4:+61910888 | None:intergenic |
| AATGTGAGAGGCAGAATTGG+AGG | 0.484111 | 5.4:-61898274 | MS.gene54168:CDS |
| GCTCTATGTTTCTGTGAAGA+TGG | 0.484266 | 5.4:-61910916 | MS.gene54168:CDS |
| CATTTGAAGATATGATAACT+TGG | 0.486436 | 5.4:-61902032 | MS.gene54168:CDS |
| TATTTGCCCACCACGTCCAC+TGG | 0.487089 | 5.4:+61901365 | None:intergenic |
| TACAAGATATCTACGCTGGT+TGG | 0.487840 | 5.4:-61904333 | MS.gene54168:CDS |
| TTGGAGAACTTGAGGGAGCT+GGG | 0.488568 | 5.4:+61908247 | None:intergenic |
| ATATGTGGAATTTCTTTCAA+AGG | 0.489523 | 5.4:+61896040 | None:intergenic |
| ACAAGATATCTACGCTGGTT+GGG | 0.493040 | 5.4:-61904332 | MS.gene54168:CDS |
| TTGTTCTTCTGCATTGCTTG+GGG | 0.498129 | 5.4:-61910847 | MS.gene54168:CDS |
| AATTGTTCGCTGTAGTGTAC+TGG | 0.501882 | 5.4:+61896784 | None:intergenic |
| TTGGATGCAAGAAGGTGGTC+AGG | 0.501888 | 5.4:-61902013 | MS.gene54168:intron |
| AACATAACCACTGTCATCTC+CGG | 0.505169 | 5.4:+61908143 | None:intergenic |
| CAGACACCCTAGATGGCTCC+AGG | 0.507459 | 5.4:+61905023 | None:intergenic |
| GATGAATTTGGAGAACTTGA+GGG | 0.507843 | 5.4:+61908240 | None:intergenic |
| AAAACACTTCAACGTTGGTA+AGG | 0.507923 | 5.4:-61904300 | MS.gene54168:intron |
| ACTATAATAGAGATAGAGTT+GGG | 0.510896 | 5.4:-61895684 | MS.gene54168:CDS |
| TTCATCCTTCGGCTGTACGC+GGG | 0.512130 | 5.4:-61909367 | MS.gene54168:CDS |
| TACCCACTATTCCGTGATCT+GGG | 0.513559 | 5.4:+61908186 | None:intergenic |
| TCATATATTACCTCCATATG+TGG | 0.516009 | 5.4:+61896025 | None:intergenic |
| ACATACACTTCTGCATAGTC+AGG | 0.517603 | 5.4:+61901648 | None:intergenic |
| CAATATCAGTGGCTACCTAC+CGG | 0.518287 | 5.4:-61899317 | MS.gene54168:CDS |
| TTTCATCCTTCGGCTGTACG+CGG | 0.520108 | 5.4:-61909368 | MS.gene54168:CDS |
| TAGTTTGGACACTGATACCG+AGG | 0.525861 | 5.4:-61904852 | MS.gene54168:intron |
| GCACTTGATGAGATAGATGC+CGG | 0.529329 | 5.4:-61896630 | MS.gene54168:CDS |
| CCAACACCCCTATCAACAAA+AGG | 0.533668 | 5.4:+61904926 | None:intergenic |
| AGATGAATTTGGAGAACTTG+AGG | 0.533944 | 5.4:+61908239 | None:intergenic |
| CCTGACTAGCTCCGTGCTTG+AGG | 0.534365 | 5.4:+61903872 | None:intergenic |
| TTCACCTTGTTATCTTGAGG+AGG | 0.535263 | 5.4:-61909552 | MS.gene54168:CDS |
| CAGAAACATAGAGCAATTCA+GGG | 0.535845 | 5.4:+61910925 | None:intergenic |
| AGATCACGGAATAGTGGGTA+TGG | 0.539500 | 5.4:-61908183 | MS.gene54168:CDS |
| CGCGCCCAATATTACTCACT+CGG | 0.539592 | 5.4:-61898315 | MS.gene54168:CDS |
| GCCGTTGCTGATCAGATGCT+GGG | 0.539654 | 5.4:-61904659 | MS.gene54168:CDS |
| CAATTCACCTTGTTATCTTG+AGG | 0.541525 | 5.4:-61909555 | MS.gene54168:CDS |
| CTTATTTGGACGTTATTCAA+AGG | 0.542609 | 5.4:-61896348 | MS.gene54168:intron |
| TGATCAGATGCTGGGTCCCA+AGG | 0.542618 | 5.4:-61904651 | MS.gene54168:CDS |
| CAATACCCTGGAGCCATCTA+GGG | 0.545381 | 5.4:-61905029 | MS.gene54168:CDS |
| GAGACATACTCGTACTCCTC+TGG | 0.546373 | 5.4:+61896564 | None:intergenic |
| TGATCTGGCAGCTAGTTGGA+GGG | 0.547032 | 5.4:-61909405 | MS.gene54168:CDS |
| GAGCATTGCAAGATGGTGCT+AGG | 0.549652 | 5.4:-61909505 | MS.gene54168:CDS |
| TCCGAATCGTGTACTCGTTG+CGG | 0.550051 | 5.4:-61909528 | MS.gene54168:CDS |
| AGGCATAGAGGCACGTAATG+AGG | 0.551759 | 5.4:-61902750 | MS.gene54168:intron |
| CAGCGAACAATTCTGACAGC+TGG | 0.553060 | 5.4:-61896773 | MS.gene54168:CDS |
| ATGAGAATATACCTTAATGC+AGG | 0.554934 | 5.4:+61898247 | None:intergenic |
| TAAACATTTGGCGATTGTCA+TGG | 0.557424 | 5.4:-61904624 | MS.gene54168:intron |
| AATACAAAACACTTCAACGT+TGG | 0.558740 | 5.4:-61904305 | MS.gene54168:CDS |
| AACAATGTCAGCACTGCGAA+AGG | 0.559133 | 5.4:-61904966 | MS.gene54168:CDS |
| TGTTGTTGTGGTATCTCACC+AGG | 0.561725 | 5.4:-61896198 | MS.gene54168:intron |
| TTAGGTATCGTTATCCTCGT+GGG | 0.562483 | 5.4:-61896375 | MS.gene54168:CDS |
| GAAAGAAATTCCACATATGG+AGG | 0.564239 | 5.4:-61896035 | MS.gene54168:intron |
| TCAGCACTGCGAAAGGAAGA+TGG | 0.564714 | 5.4:-61904959 | MS.gene54168:CDS |
| TACTATAATAGAGATAGAGT+TGG | 0.565226 | 5.4:-61895685 | MS.gene54168:CDS |
| TCACCTTGTTATCTTGAGGA+GGG | 0.565452 | 5.4:-61909551 | MS.gene54168:CDS |
| CCCGCAACGAGTACACGATT+CGG | 0.568886 | 5.4:+61909527 | None:intergenic |
| AAAGATTTCCAGCGATAGCG+CGG | 0.570884 | 5.4:+61910883 | None:intergenic |
| TTCTGAATTTATGCTGACGT+CGG | 0.571325 | 5.4:+61909340 | None:intergenic |
| CCAACTAGCTGCCAGATCAA+AGG | 0.574144 | 5.4:+61909409 | None:intergenic |
| ATTGACATGGCCAGTGGACG+TGG | 0.575757 | 5.4:-61901375 | MS.gene54168:CDS |
| ACTTGTTAAATCTATGTCAG+TGG | 0.577772 | 5.4:-61904990 | MS.gene54168:CDS |
| CTTGTTAAATCTATGTCAGT+GGG | 0.579027 | 5.4:-61904989 | MS.gene54168:CDS |
| TTTCGAGCTGACAATCCTGA+AGG | 0.580736 | 5.4:-61902770 | MS.gene54168:CDS |
| CACTTGATGAGATAGATGCC+GGG | 0.581155 | 5.4:-61896629 | MS.gene54168:CDS |
| GGCAGCAATTCATATTCCGC+CGG | 0.582198 | 5.4:-61908162 | MS.gene54168:CDS |
| ATAGATGCCGGGGTGTGTGA+TGG | 0.582570 | 5.4:-61896618 | MS.gene54168:CDS |
| ATCTCTATTATAGTATGAAG+TGG | 0.583438 | 5.4:+61895693 | None:intergenic |
| ATCCCAGATCACGGAATAGT+GGG | 0.585832 | 5.4:-61908188 | MS.gene54168:CDS |
| GACGAAGAACACGACGGTAA+CGG | 0.588194 | 5.4:-61910972 | MS.gene54168:CDS |
| GCAACGAGCACCTGTTGTTG+TGG | 0.589016 | 5.4:-61896210 | MS.gene54168:CDS |
| CAGTATCGTCGCCTCAAGCA+CGG | 0.589203 | 5.4:-61903883 | MS.gene54168:CDS |
| GGTTTATGAGACGGTAGAAG+AGG | 0.591074 | 5.4:-61901415 | MS.gene54168:CDS |
| AATATCAGTGGCTACCTACC+GGG | 0.595215 | 5.4:-61899316 | MS.gene54168:CDS |
| ACCCAGCATCTGATCAGCAA+CGG | 0.597212 | 5.4:+61904658 | None:intergenic |
| ATGATAACTTGGATGCAAGA+AGG | 0.600170 | 5.4:-61902021 | MS.gene54168:CDS |
| TGACAATCCTGAAGGCATAG+AGG | 0.600699 | 5.4:-61902762 | MS.gene54168:CDS |
| CTGTAATTATTGAGCTTGAG+CGG | 0.602405 | 5.4:-61896232 | MS.gene54168:CDS |
| AAAAGACAATTCTGCCCGGT+AGG | 0.604777 | 5.4:+61899302 | None:intergenic |
| AACCTTAGGAAATCCAACAA+TGG | 0.605128 | 5.4:+61896748 | None:intergenic |
| ATGTTCCAATTAGGAGATGG+AGG | 0.605793 | 5.4:-61905053 | MS.gene54168:intron |
| GCGAAAGGAAGATGGTCAGA+GGG | 0.609079 | 5.4:-61904951 | MS.gene54168:CDS |
| CTTAGGTATCGTTATCCTCG+TGG | 0.609423 | 5.4:-61896376 | MS.gene54168:CDS |
| GGAGATGGAGGTCAATACCC+TGG | 0.612275 | 5.4:-61905041 | MS.gene54168:CDS |
| GTTTATGAGACGGTAGAAGA+GGG | 0.613100 | 5.4:-61901414 | MS.gene54168:CDS |
| TCGTGTAGCCAATTATGAGA+AGG | 0.618596 | 5.4:-61901517 | MS.gene54168:intron |
| TGCGAAAGGAAGATGGTCAG+AGG | 0.619804 | 5.4:-61904952 | MS.gene54168:CDS |
| ATGATGTAGACATGATTGAA+AGG | 0.619961 | 5.4:-61901698 | MS.gene54168:CDS |
| GGTATCCCGCGTACAGCCGA+AGG | 0.621735 | 5.4:+61909362 | None:intergenic |
| GTTGCGGGAGCATTGCAAGA+TGG | 0.623684 | 5.4:-61909512 | MS.gene54168:CDS |
| AGAAGTTCAGACAGAAAGCG+AGG | 0.629548 | 5.4:-61909321 | MS.gene54168:intron |
| TGACAGAGAATCCCAGATCA+CGG | 0.634764 | 5.4:-61908197 | MS.gene54168:CDS |
| CCAATATTACTCACTCGGCA+TGG | 0.636693 | 5.4:-61898310 | MS.gene54168:CDS |
| GTTTAGGTGAACAATATCAG+TGG | 0.636959 | 5.4:-61899328 | MS.gene54168:intron |
| AAAATGATTGACATGGCCAG+TGG | 0.637498 | 5.4:-61901381 | MS.gene54168:CDS |
| GAAAGTCAGGATAATGTGAG+AGG | 0.637995 | 5.4:-61898286 | MS.gene54168:CDS |
| ATTGGCTGAAGGAAGATGCA+AGG | 0.643465 | 5.4:-61901853 | MS.gene54168:intron |
| AACTTACAAGATATCTACGC+TGG | 0.644186 | 5.4:-61904337 | MS.gene54168:CDS |
| TGTCATTCCATCACACACCC+CGG | 0.646684 | 5.4:+61896611 | None:intergenic |
| CGAGTGAGTAATATTGGGCG+CGG | 0.647921 | 5.4:+61898316 | None:intergenic |
| TAGGTGAGTCTGCCAGCTCG+AGG | 0.649671 | 5.4:-61904380 | MS.gene54168:CDS |
| AGCAATTCAGGGATTCGTAA+CGG | 0.650543 | 5.4:+61910936 | None:intergenic |
| GACATGGCCAGTGGACGTGG+TGG | 0.651270 | 5.4:-61901372 | MS.gene54168:CDS |
| ATATTACTAGGTTTATGAGA+CGG | 0.659393 | 5.4:-61901424 | MS.gene54168:intron |
| AATCAAGAAGAACATGCCAG+AGG | 0.659584 | 5.4:-61896580 | MS.gene54168:CDS |
| ATGCTGATGAAATTGGCTGA+AGG | 0.659984 | 5.4:-61901864 | MS.gene54168:CDS |
| GGACCCTCCTCAAGATAACA+AGG | 0.663997 | 5.4:+61909548 | None:intergenic |
| AGACACCCTAGATGGCTCCA+GGG | 0.667988 | 5.4:+61905024 | None:intergenic |
| TAGGTATCGTTATCCTCGTG+GGG | 0.669051 | 5.4:-61896374 | MS.gene54168:CDS |
| ATAACTTGGATGCAAGAAGG+TGG | 0.670683 | 5.4:-61902018 | MS.gene54168:CDS |
| ACATGGCCAGTGGACGTGGT+GGG | 0.683504 | 5.4:-61901371 | MS.gene54168:CDS |
| TATTCCGCCGGAGATGACAG+TGG | 0.687762 | 5.4:-61908150 | MS.gene54168:CDS |
| TCCAAATAAGACTCCCCACG+AGG | 0.688783 | 5.4:+61896361 | None:intergenic |
| ATAACCACTGTCATCTCCGG+CGG | 0.696409 | 5.4:+61908146 | None:intergenic |
| TCAGATGCTGGGTCCCAAGG+AGG | 0.702698 | 5.4:-61904648 | MS.gene54168:CDS |
| ACTTGATGAGATAGATGCCG+GGG | 0.731577 | 5.4:-61896628 | MS.gene54168:CDS |
| CGAAAGGAAGATGGTCAGAG+GGG | 0.735126 | 5.4:-61904950 | MS.gene54168:CDS |
| CCTTAGGAAATCCAACAATG+GGG | 0.750097 | 5.4:+61896750 | None:intergenic |
| ATTAGCGACGAAGAACACGA+CGG | 0.789281 | 5.4:-61910978 | MS.gene54168:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 61895641 | 61911009 | 61895641 | ID=MS.gene54168 |
| chr5.4 | mRNA | 61895641 | 61911009 | 61895641 | ID=MS.gene54168.t1;Parent=MS.gene54168 |
| chr5.4 | exon | 61910796 | 61911009 | 61910796 | ID=MS.gene54168.t1.exon1;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61910796 | 61911009 | 61910796 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61909322 | 61909617 | 61909322 | ID=MS.gene54168.t1.exon2;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61909322 | 61909617 | 61909322 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61908128 | 61908316 | 61908128 | ID=MS.gene54168.t1.exon3;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61908128 | 61908316 | 61908128 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61904853 | 61905062 | 61904853 | ID=MS.gene54168.t1.exon4;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61904853 | 61905062 | 61904853 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61904625 | 61904720 | 61904625 | ID=MS.gene54168.t1.exon5;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61904625 | 61904720 | 61904625 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61904301 | 61904399 | 61904301 | ID=MS.gene54168.t1.exon6;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61904301 | 61904399 | 61904301 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61903873 | 61903902 | 61903873 | ID=MS.gene54168.t1.exon7;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61903873 | 61903902 | 61903873 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61902751 | 61902804 | 61902751 | ID=MS.gene54168.t1.exon8;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61902751 | 61902804 | 61902751 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61902014 | 61902067 | 61902014 | ID=MS.gene54168.t1.exon9;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61902014 | 61902067 | 61902014 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61901854 | 61901931 | 61901854 | ID=MS.gene54168.t1.exon10;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61901854 | 61901931 | 61901854 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61901654 | 61901745 | 61901654 | ID=MS.gene54168.t1.exon11;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61901654 | 61901745 | 61901654 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61901518 | 61901575 | 61901518 | ID=MS.gene54168.t1.exon12;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61901518 | 61901575 | 61901518 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61901362 | 61901436 | 61901362 | ID=MS.gene54168.t1.exon13;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61901362 | 61901436 | 61901362 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61899297 | 61899344 | 61899297 | ID=MS.gene54168.t1.exon14;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61899297 | 61899344 | 61899297 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61898259 | 61898356 | 61898259 | ID=MS.gene54168.t1.exon15;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61898259 | 61898356 | 61898259 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61897369 | 61897444 | 61897369 | ID=MS.gene54168.t1.exon16;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61897369 | 61897444 | 61897369 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61896751 | 61896813 | 61896751 | ID=MS.gene54168.t1.exon17;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61896751 | 61896813 | 61896751 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61896573 | 61896664 | 61896573 | ID=MS.gene54168.t1.exon18;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61896573 | 61896664 | 61896573 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61896349 | 61896417 | 61896349 | ID=MS.gene54168.t1.exon19;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61896349 | 61896417 | 61896349 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61896199 | 61896262 | 61896199 | ID=MS.gene54168.t1.exon20;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61896199 | 61896262 | 61896199 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61896036 | 61896101 | 61896036 | ID=MS.gene54168.t1.exon21;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61896036 | 61896101 | 61896036 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| chr5.4 | exon | 61895641 | 61895718 | 61895641 | ID=MS.gene54168.t1.exon22;Parent=MS.gene54168.t1 |
| chr5.4 | CDS | 61895641 | 61895718 | 61895641 | ID=cds.MS.gene54168.t1;Parent=MS.gene54168.t1 |
| Gene Sequence |
| Protein sequence |
