AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82543


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82543.t1 MTR_3g070960 95.238 231 11 0 1 231 1 231 2.34e-162 448
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82543.t1 AT3G56010 58.519 135 44 4 99 231 77 201 3.42e-48 157

Find 53 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTCATTAGTCATTAAAA+TGG 0.237690 3.1:-58224060 None:intergenic
AACAAATTACCAGGGGCTTC+TGG 0.243956 3.1:-58223890 None:intergenic
TTCAACAGATGCTAGTAATA+AGG 0.322512 3.1:+58223583 MS.gene82543:CDS
TGAGATGATCGTGTGGTTAC+AGG 0.325931 3.1:-58223725 None:intergenic
TTTGTCAAGGAGAAAAGATT+TGG 0.326672 3.1:+58223527 MS.gene82543:CDS
TTTATCAGCACAGTGATGTT+TGG 0.378545 3.1:+58224004 MS.gene82543:CDS
GTGTGCTCTGAGTGGCCTTC+GGG 0.390366 3.1:-58223475 None:intergenic
AGAAAGTGAGAGAGAGGATA+AGG 0.396564 3.1:+58223747 MS.gene82543:CDS
TCAAAAGTCCAACCTTCCTT+TGG 0.411926 3.1:-58223935 None:intergenic
AGTGTGCTCTGAGTGGCCTT+CGG 0.416107 3.1:-58223476 None:intergenic
GTCTGTGATTCTGCCTGAGC+AGG 0.422298 3.1:-58223659 None:intergenic
GTGGTTACAGGTTGCGACTT+AGG 0.426739 3.1:-58223713 None:intergenic
GGTAGATGTTAACTGATCAT+TGG 0.433318 3.1:-58223633 None:intergenic
CAAACTATCACGATGCATCT+AGG 0.448156 3.1:+58223432 MS.gene82543:CDS
CTGTCATCAAACAAATTACC+AGG 0.451100 3.1:-58223899 None:intergenic
GATGCTAGTAATAAGGATAC+TGG 0.451608 3.1:+58223590 MS.gene82543:CDS
GGCCTTCGGGAAAGAAGCTC+CGG 0.457076 3.1:-58223462 None:intergenic
GAAAGTGAGAGAGAGGATAA+GGG 0.460702 3.1:+58223748 MS.gene82543:CDS
TGATTCTGCCTGAGCAGGAT+TGG 0.469008 3.1:-58223654 None:intergenic
AAAACAGCTAAATCAGAAAT+GGG 0.480346 3.1:+58223803 MS.gene82543:CDS
CGTTGAGGTGAAGCTGATGA+TGG 0.495454 3.1:-58222478 None:intergenic
GCAGATCCATTGGAAACCTC+TGG 0.503333 3.1:-58223683 None:intergenic
AACATCTACCAATCCTGCTC+AGG 0.512154 3.1:+58223646 MS.gene82543:CDS
AGGAGATGCTGCAGATCCAT+TGG 0.516503 3.1:-58223693 None:intergenic
CTAGTAATAAGGATACTGGC+AGG 0.528322 3.1:+58223594 MS.gene82543:CDS
TGCCGGAGCTTCTTTCCCGA+AGG 0.532920 3.1:+58223460 MS.gene82543:CDS
ACTTACATTGTGTGGAAAGT+TGG 0.543190 3.1:+58224031 MS.gene82543:CDS
TTGGAACAACTTACATTGTG+TGG 0.560213 3.1:+58224023 MS.gene82543:CDS
GAGGGATGCCAAAGGAAGGT+TGG 0.568563 3.1:+58223927 MS.gene82543:CDS
CATCTCAGAAAGTGAGAGAG+AGG 0.580497 3.1:+58223741 MS.gene82543:CDS
GTTGAGGTGAAGCTGATGAT+GGG 0.590259 3.1:-58222477 None:intergenic
ACACTCGTTTCGATAACGTC+GGG 0.594355 3.1:+58223494 MS.gene82543:CDS
TTGTTTGATGACAGCAAACG+AGG 0.599920 3.1:+58223908 MS.gene82543:CDS
TCTGGACTTCCAGAAGCCCC+TGG 0.604678 3.1:+58223881 MS.gene82543:CDS
GATGCATCTAGGTATTCTGC+CGG 0.605736 3.1:+58223443 MS.gene82543:CDS
CACTTTCTGAGATGATCGTG+TGG 0.606839 3.1:-58223732 None:intergenic
AAACGAGGGATGCCAAAGGA+AGG 0.625366 3.1:+58223923 MS.gene82543:CDS
TCGAGTTCTTAACCAGTCCA+AGG 0.632326 3.1:+58223775 MS.gene82543:CDS
CATCATCAGCTTCACCTCAA+CGG 0.635332 3.1:+58222479 MS.gene82543:CDS
TAGTAATAAGGATACTGGCA+GGG 0.635455 3.1:+58223595 MS.gene82543:CDS
GGGAACAAGAATGTTTGTCA+AGG 0.643523 3.1:+58223514 MS.gene82543:CDS
CAGCAAACGAGGGATGCCAA+AGG 0.649716 3.1:+58223919 MS.gene82543:CDS
CAGACACCAGAGGTTTCCAA+TGG 0.649775 3.1:+58223677 MS.gene82543:CDS
AGAAAGAGATCAGAACCACC+AGG 0.655199 3.1:-58223962 None:intergenic
TGTTTGATGACAGCAAACGA+GGG 0.666281 3.1:+58223909 MS.gene82543:CDS
AGAAATGGGAAGTAGTGTGT+TGG 0.668878 3.1:+58223817 MS.gene82543:CDS
CACACTCGTTTCGATAACGT+CGG 0.679278 3.1:+58223493 MS.gene82543:CDS
GGCAGAATCACAGACACCAG+AGG 0.679651 3.1:+58223667 MS.gene82543:CDS
TGTCATCAAACAAATTACCA+GGG 0.683437 3.1:-58223898 None:intergenic
AGTGAGAGAGAGGATAAGGG+CGG 0.687543 3.1:+58223751 MS.gene82543:CDS
TTGAGGTGAAGCTGATGATG+GGG 0.696015 3.1:-58222476 None:intergenic
GTCATCAAACAAATTACCAG+GGG 0.699685 3.1:-58223897 None:intergenic
CGAAACGAGTGTGCTCTGAG+TGG 0.707929 3.1:-58223483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAATTGAATTATATTGA+AGG - chr3.1:58223347-58223366 None:intergenic 15.0%
!! ATTATCTTCTAATATGTAAT+AGG + chr3.1:58223287-58223306 MS.gene82543:intron 15.0%
!! AAGGAAATATGACTATTTAA+AGG - chr3.1:58223007-58223026 None:intergenic 20.0%
!! AGGAAATATGACTATTTAAA+GGG - chr3.1:58223006-58223025 None:intergenic 20.0%
!! ATACTAAACATTATTTGACT+TGG + chr3.1:58222673-58222692 MS.gene82543:intron 20.0%
!! GTAACAAGTTTATAGATTTA+AGG + chr3.1:58222582-58222601 MS.gene82543:intron 20.0%
!! TGTAAAAAGTAACAAATATG+TGG - chr3.1:58222560-58222579 None:intergenic 20.0%
!!! AGTTTATAGATTTAAGGTTA+TGG + chr3.1:58222588-58222607 MS.gene82543:intron 20.0%
!!! TACTAAACATTATTTGACTT+GGG + chr3.1:58222674-58222693 MS.gene82543:intron 20.0%
! AAAAACAGCTAAATCAGAAA+TGG + chr3.1:58223802-58223821 MS.gene82543:CDS 25.0%
! AAAACAGCTAAATCAGAAAT+GGG + chr3.1:58223803-58223822 MS.gene82543:CDS 25.0%
! CCCAAAATTGACATATAAAA+GGG - chr3.1:58223246-58223265 None:intergenic 25.0%
! TAAATAGTCATATTTCCTTG+TGG + chr3.1:58223008-58223027 MS.gene82543:intron 25.0%
! TAACAACCTGTTATGTTAAT+CGG + chr3.1:58222744-58222763 MS.gene82543:intron 25.0%
! TATAAAATGCGCTATATCTA+TGG + chr3.1:58222831-58222850 MS.gene82543:intron 25.0%
! TATTTAAAGGGCTAATTCTA+CGG - chr3.1:58222994-58223013 None:intergenic 25.0%
! TCCCAAAATTGACATATAAA+AGG - chr3.1:58223247-58223266 None:intergenic 25.0%
! TTAACATAAAGCAATTTCAC+TGG - chr3.1:58222811-58222830 None:intergenic 25.0%
!! ATTTGCTTGTAATTGATTCT+TGG + chr3.1:58222644-58222663 MS.gene82543:intron 25.0%
!! CCCTTTTATATGTCAATTTT+GGG + chr3.1:58223243-58223262 MS.gene82543:intron 25.0%
!! TCCCTTTTATATGTCAATTT+TGG + chr3.1:58223242-58223261 MS.gene82543:intron 25.0%
!!! ATTTCTGATTTAGCTGTTTT+TGG - chr3.1:58223803-58223822 None:intergenic 25.0%
!!! TTGTTTGATGATGAAAATGT+TGG + chr3.1:58223195-58223214 MS.gene82543:intron 25.0%
ATCTCAATTGATCCAATTGA+AGG - chr3.1:58222919-58222938 None:intergenic 30.0%
CAATTGGATCAATTGAGATA+TGG + chr3.1:58222920-58222939 MS.gene82543:intron 30.0%
CACTATTTGCAACTGAAAAA+CGG - chr3.1:58223556-58223575 None:intergenic 30.0%
TGAAGGACTTATTGATCAAA+TGG - chr3.1:58222902-58222921 None:intergenic 30.0%
TGATCAATAAGTCCTTCAAT+TGG + chr3.1:58222904-58222923 MS.gene82543:intron 30.0%
TGTCATCAAACAAATTACCA+GGG - chr3.1:58223901-58223920 None:intergenic 30.0%
TTCAACAGATGCTAGTAATA+AGG + chr3.1:58223583-58223602 MS.gene82543:CDS 30.0%
TTTCATTATCCCTTAGAAGT+TGG + chr3.1:58223406-58223425 MS.gene82543:intron 30.0%
TTTGTCAAGGAGAAAAGATT+TGG + chr3.1:58223527-58223546 MS.gene82543:CDS 30.0%
! AGTTTGTTTCCAACTTCTAA+GGG - chr3.1:58223418-58223437 None:intergenic 30.0%
! TAAGAAGAGTGTGTAAGTTA+TGG + chr3.1:58222611-58222630 MS.gene82543:intron 30.0%
!! CTTTTAAAGAAAGTGACAGA+GGG + chr3.1:58223843-58223862 MS.gene82543:CDS 30.0%
!! GGTGAGATTTATTGATTCAA+TGG + chr3.1:58222941-58222960 MS.gene82543:intron 30.0%
!! TAGTTTGTTTCCAACTTCTA+AGG - chr3.1:58223419-58223438 None:intergenic 30.0%
ACTTACATTGTGTGGAAAGT+TGG + chr3.1:58224031-58224050 MS.gene82543:CDS 35.0%
CTGTCATCAAACAAATTACC+AGG - chr3.1:58223902-58223921 None:intergenic 35.0%
CTTTGCAGTTATGTTGTGAT+CGG + chr3.1:58223144-58223163 MS.gene82543:intron 35.0%
GAACTTAACAAAGATCCACA+AGG - chr3.1:58223026-58223045 None:intergenic 35.0%
GATGCTAGTAATAAGGATAC+TGG + chr3.1:58223590-58223609 MS.gene82543:CDS 35.0%
GGTAGATGTTAACTGATCAT+TGG - chr3.1:58223636-58223655 None:intergenic 35.0%
GTCATCAAACAAATTACCAG+GGG - chr3.1:58223900-58223919 None:intergenic 35.0%
TAATCGGACACAATATTCCT+GGG + chr3.1:58222760-58222779 MS.gene82543:intron 35.0%
TAGTAATAAGGATACTGGCA+GGG + chr3.1:58223595-58223614 MS.gene82543:CDS 35.0%
TTAATCGGACACAATATTCC+TGG + chr3.1:58222759-58222778 MS.gene82543:intron 35.0%
TTGGAACAACTTACATTGTG+TGG + chr3.1:58224023-58224042 MS.gene82543:CDS 35.0%
TTGTGTCCGATTAACATAAC+AGG - chr3.1:58222753-58222772 None:intergenic 35.0%
TTTATCAGCACAGTGATGTT+TGG + chr3.1:58224004-58224023 MS.gene82543:CDS 35.0%
!! GCTTTTAAAGAAAGTGACAG+AGG + chr3.1:58223842-58223861 MS.gene82543:CDS 35.0%
!!! TTTAGCTGTTTTTGGTTCCT+TGG - chr3.1:58223795-58223814 None:intergenic 35.0%
AAAGTGACAGAGGGAAAAAG+AGG + chr3.1:58223852-58223871 MS.gene82543:CDS 40.0%
AGAAAGTGAGAGAGAGGATA+AGG + chr3.1:58223747-58223766 MS.gene82543:CDS 40.0%
AGAAATGGGAAGTAGTGTGT+TGG + chr3.1:58223817-58223836 MS.gene82543:CDS 40.0%
CAAACTATCACGATGCATCT+AGG + chr3.1:58223432-58223451 MS.gene82543:CDS 40.0%
CATTAAACCAAAGTTCCAGG+TGG + chr3.1:58223062-58223081 MS.gene82543:intron 40.0%
CTAGTAATAAGGATACTGGC+AGG + chr3.1:58223594-58223613 MS.gene82543:CDS 40.0%
CTATCATAAGAAGTTCACCC+AGG - chr3.1:58222780-58222799 None:intergenic 40.0%
CTATGGTGCACATGTTAAGT+AGG + chr3.1:58222848-58222867 MS.gene82543:intron 40.0%
GAAAGTGAGAGAGAGGATAA+GGG + chr3.1:58223748-58223767 MS.gene82543:CDS 40.0%
GATAGAAAAACGTACCGTTG+AGG - chr3.1:58222496-58222515 None:intergenic 40.0%
GGGAACAAGAATGTTTGTCA+AGG + chr3.1:58223514-58223533 MS.gene82543:CDS 40.0%
GTGCATTAAACCAAAGTTCC+AGG + chr3.1:58223059-58223078 MS.gene82543:intron 40.0%
TCAAAAGTCCAACCTTCCTT+TGG - chr3.1:58223938-58223957 None:intergenic 40.0%
! GTTAAGTAGGTGCTGTACTA+TGG + chr3.1:58222861-58222880 MS.gene82543:intron 40.0%
! TGTTTGATGACAGCAAACGA+GGG + chr3.1:58223909-58223928 MS.gene82543:CDS 40.0%
! TTGTTTGATGACAGCAAACG+AGG + chr3.1:58223908-58223927 MS.gene82543:CDS 40.0%
!!! GGTTGGACTTTTGATTTTCC+TGG + chr3.1:58223944-58223963 MS.gene82543:CDS 40.0%
!!! TGGACTTTTGATTTTCCTGG+TGG + chr3.1:58223947-58223966 MS.gene82543:CDS 40.0%
AACAAATTACCAGGGGCTTC+TGG - chr3.1:58223893-58223912 None:intergenic 45.0%
AACATCTACCAATCCTGCTC+AGG + chr3.1:58223646-58223665 MS.gene82543:CDS 45.0%
ACACTCGTTTCGATAACGTC+GGG + chr3.1:58223494-58223513 MS.gene82543:CDS 45.0%
AGAAAGAGATCAGAACCACC+AGG - chr3.1:58223965-58223984 None:intergenic 45.0%
AGAAAGTCCACCTGGAACTT+TGG - chr3.1:58223072-58223091 None:intergenic 45.0%
CACACTCGTTTCGATAACGT+CGG + chr3.1:58223493-58223512 MS.gene82543:CDS 45.0%
CATCATCAGCTTCACCTCAA+CGG + chr3.1:58222479-58222498 MS.gene82543:CDS 45.0%
CATCTCAGAAAGTGAGAGAG+AGG + chr3.1:58223741-58223760 MS.gene82543:CDS 45.0%
GATGCATCTAGGTATTCTGC+CGG + chr3.1:58223443-58223462 MS.gene82543:CDS 45.0%
GGGAAAAAGAGGAGAAGATC+TGG + chr3.1:58223863-58223882 MS.gene82543:CDS 45.0%
GTTGAGGTGAAGCTGATGAT+GGG - chr3.1:58222480-58222499 None:intergenic 45.0%
TCGAGTTCTTAACCAGTCCA+AGG + chr3.1:58223775-58223794 MS.gene82543:CDS 45.0%
TGAGATGATCGTGTGGTTAC+AGG - chr3.1:58223728-58223747 None:intergenic 45.0%
! CATTCTAGAGAAAGTCCACC+TGG - chr3.1:58223080-58223099 None:intergenic 45.0%
! TTGAGGTGAAGCTGATGATG+GGG - chr3.1:58222479-58222498 None:intergenic 45.0%
!! CACTTTCTGAGATGATCGTG+TGG - chr3.1:58223735-58223754 None:intergenic 45.0%
!!! CTGTTTTTGGTTCCTTGGAC+TGG - chr3.1:58223790-58223809 None:intergenic 45.0%
AAACGAGGGATGCCAAAGGA+AGG + chr3.1:58223923-58223942 MS.gene82543:CDS 50.0%
AGGAGATGCTGCAGATCCAT+TGG - chr3.1:58223696-58223715 None:intergenic 50.0%
AGTGAGAGAGAGGATAAGGG+CGG + chr3.1:58223751-58223770 MS.gene82543:CDS 50.0%
CAGACACCAGAGGTTTCCAA+TGG + chr3.1:58223677-58223696 MS.gene82543:CDS 50.0%
CGTTGAGGTGAAGCTGATGA+TGG - chr3.1:58222481-58222500 None:intergenic 50.0%
GCAGATCCATTGGAAACCTC+TGG - chr3.1:58223686-58223705 None:intergenic 50.0%
GTGGTTACAGGTTGCGACTT+AGG - chr3.1:58223716-58223735 None:intergenic 50.0%
TGATTCTGCCTGAGCAGGAT+TGG - chr3.1:58223657-58223676 None:intergenic 50.0%
CAGCAAACGAGGGATGCCAA+AGG + chr3.1:58223919-58223938 MS.gene82543:CDS 55.0%
GAGGGATGCCAAAGGAAGGT+TGG + chr3.1:58223927-58223946 MS.gene82543:CDS 55.0%
GGCAGAATCACAGACACCAG+AGG + chr3.1:58223667-58223686 MS.gene82543:CDS 55.0%
GTCTGTGATTCTGCCTGAGC+AGG - chr3.1:58223662-58223681 None:intergenic 55.0%
! AGTGTGCTCTGAGTGGCCTT+CGG - chr3.1:58223479-58223498 None:intergenic 55.0%
! CGAAACGAGTGTGCTCTGAG+TGG - chr3.1:58223486-58223505 None:intergenic 55.0%
GGCCTTCGGGAAAGAAGCTC+CGG - chr3.1:58223465-58223484 None:intergenic 60.0%
TCTGGACTTCCAGAAGCCCC+TGG + chr3.1:58223881-58223900 MS.gene82543:CDS 60.0%
TGCCGGAGCTTCTTTCCCGA+AGG + chr3.1:58223460-58223479 MS.gene82543:CDS 60.0%
! GTGTGCTCTGAGTGGCCTTC+GGG - chr3.1:58223478-58223497 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 58222459 58224075 58222459 ID=MS.gene82543
chr3.1 mRNA 58222459 58224075 58222459 ID=MS.gene82543.t1;Parent=MS.gene82543
chr3.1 exon 58222459 58222500 58222459 ID=MS.gene82543.t1.exon1;Parent=MS.gene82543.t1
chr3.1 CDS 58222459 58222500 58222459 ID=cds.MS.gene82543.t1;Parent=MS.gene82543.t1
chr3.1 exon 58223422 58224075 58223422 ID=MS.gene82543.t1.exon2;Parent=MS.gene82543.t1
chr3.1 CDS 58223422 58224075 58223422 ID=cds.MS.gene82543.t1;Parent=MS.gene82543.t1
Gene Sequence

>MS.gene82543

ATGGCTATATCTCTAAACCCCATCATCAGCTTCACCTCAACGGTACGTTTTTCTATCTTCTCTTACTTTCTAGTATTCTCTAATCACTTTAGTTTCTTCCACATATTTGTTACTTTTTACAGTGTAACAAGTTTATAGATTTAAGGTTATGGTAAGAAGAGTGTGTAAGTTATGGAAATTTACGTATTTGCTTGTAATTGATTCTTGGTTCTGCATACTAAACATTATTTGACTTGGGTTTTTTAGTAATTATATGTGTTGAGAAGCACATATATTTAACAAACTTAACAACCTGTTATGTTAATCGGACACAATATTCCTGGGTGAACTTCTTATGATAGTAATTGTTCCAGTGAAATTGCTTTATGTTAATATAAAATGCGCTATATCTATGGTGCACATGTTAAGTAGGTGCTGTACTATGGAAAAGTTTGTTTCTACCATTTGATCAATAAGTCCTTCAATTGGATCAATTGAGATATGGTGAGATTTATTGATTCAATGGTAAAAAAACTCGTGTTTTACTTGTGCACCGTAGAATTAGCCCTTTAAATAGTCATATTTCCTTGTGGATCTTTGTTAAGTTCATGTTTTGTCAAAGTGCATTAAACCAAAGTTCCAGGTGGACTTTCTCTAGAATGTATGTAGTACTGTAATTCTGTGAAGACAAATTTATTGATTGTAGCTTTGCAGTTATGTTGTGATCGGTTCTTAAGTTATGTATTTGTTAAGAGAGTTGTTTGATGATGAAAATGTTGGTTTAGCTATTGATCAGATTCAGAGTCCCTTTTATATGTCAATTTTGGGATTATTACTCTGAAAAATAAAATTATCTTCTAATATGTAATAGGTTATTAATAACTGTTATTGTGTTTAACATCAATTCCTTCAATATAATTCAATTATGTTAATTTTAACTCTCATTTTTCTGATCACTCACACAAGTCTTTCATTATCCCTTAGAAGTTGGAAACAAACTATCACGATGCATCTAGGTATTCTGCCGGAGCTTCTTTCCCGAAGGCCACTCAGAGCACACTCGTTTCGATAACGTCGGGAACAAGAATGTTTGTCAAGGAGAAAAGATTTGGTCACCGTTTTTCAGTTGCAAATAGTGATCAACTTTCAACAGATGCTAGTAATAAGGATACTGGCAGGGCTGCAGATTCATCTGCCAATGATCAGTTAACATCTACCAATCCTGCTCAGGCAGAATCACAGACACCAGAGGTTTCCAATGGATCTGCAGCATCTCCTAAGTCGCAACCTGTAACCACACGATCATCTCAGAAAGTGAGAGAGAGGATAAGGGCGGCTCGAGTTCTTAACCAGTCCAAGGAACCAAAAACAGCTAAATCAGAAATGGGAAGTAGTGTGTTGGCTGCTTTTAAAGAAAGTGACAGAGGGAAAAAGAGGAGAAGATCTGGACTTCCAGAAGCCCCTGGTAATTTGTTTGATGACAGCAAACGAGGGATGCCAAAGGAAGGTTGGACTTTTGATTTTCCTGGTGGTTCTGATCTCTTTCTGATTATATTTTCTTTTGTCTTTATCAGCACAGTGATGTTTGGAACAACTTACATTGTGTGGAAAGTTGGTGCAATCCATTTTAATGACTAA

Protein sequence

>MS.gene82543.t1

MAISLNPIISFTSTKLETNYHDASRYSAGASFPKATQSTLVSITSGTRMFVKEKRFGHRFSVANSDQLSTDASNKDTGRAADSSANDQLTSTNPAQAESQTPEVSNGSAASPKSQPVTTRSSQKVRERIRAARVLNQSKEPKTAKSEMGSSVLAAFKESDRGKKRRRSGLPEAPGNLFDDSKRGMPKEGWTFDFPGGSDLFLIIFSFVFISTVMFGTTYIVWKVGAIHFND