AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92135


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92135.t1 MTR_8g028180 98.630 146 2 0 1 146 1 146 3.24e-101 286
MS.gene92135.t1 MTR_3g005520 83.333 150 21 1 1 146 1 150 2.32e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92135.t1 AT2G04520 87.671 146 17 1 1 146 1 145 8.27e-85 245
MS.gene92135.t1 AT5G35680 86.986 146 18 1 1 146 1 145 7.68e-80 232
MS.gene92135.t1 AT5G35680 86.986 146 18 1 1 146 1 145 7.68e-80 232
MS.gene92135.t1 AT5G35680 93.277 119 8 0 1 119 1 119 6.39e-78 228

Find 48 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGGTTGCGTGATTATC+AGG 0.308178 8.3:+65471290 MS.gene92135:CDS
TCATTAAGCCTCACACCTTC+AGG 0.319525 8.3:-65471384 None:intergenic
CCTCCCTTTCCCTTGTTCTT+CGG 0.331257 8.3:-65471060 None:intergenic
GCTGGGGATATTATTCTTGT+TGG 0.331915 8.3:+65471273 MS.gene92135:CDS
GGAATTGGTGGTGGGATTGA+TGG 0.360212 8.3:+65471408 MS.gene92135:CDS
CTGGGGATATTATTCTTGTT+GGG 0.363705 8.3:+65471274 MS.gene92135:CDS
GAGGATATTGATAAGATTTG+AGG 0.399236 8.3:+65471477 MS.gene92135:CDS
GAAAGATGCACAAGAAGGTT+TGG 0.408452 8.3:+65471244 MS.gene92135:CDS
CTTTCCCTTGTTCTTCGGCA+TGG 0.425552 8.3:-65471055 None:intergenic
GAATTGGTGGTGGGATTGAT+GGG 0.434528 8.3:+65471409 MS.gene92135:CDS
AAGAAGGTTTGGATTGCTGC+TGG 0.450047 8.3:+65471255 MS.gene92135:CDS
TTCAACAACCTCGCCTCATC+CGG 0.452149 8.3:-65471345 None:intergenic
TTATATTGAGTTTGAGGATG+AGG 0.463979 8.3:+65471458 MS.gene92135:CDS
GCACAGGTTCTTCGTATGCT+TGG 0.465282 8.3:+65471162 MS.gene92135:CDS
GGGGATGATGATGAGGGTGA+TGG 0.471229 8.3:+65471429 MS.gene92135:CDS
GGGATGATGATGAGGGTGAT+GGG 0.477444 8.3:+65471430 MS.gene92135:CDS
AGGATATTGATAAGATTTGA+GGG 0.478420 8.3:+65471478 MS.gene92135:CDS
GAATGATTATATTGAGTTTG+AGG 0.491210 8.3:+65471452 MS.gene92135:CDS
GTGAGGCTTAATGAAGGAAT+TGG 0.513375 8.3:+65471393 MS.gene92135:CDS
CTTAATGAAGGAATTGGTGG+TGG 0.513624 8.3:+65471399 MS.gene92135:CDS
GAAGGTTTGGATTGCTGCTG+GGG 0.517248 8.3:+65471257 MS.gene92135:CDS
GCGTGATTATCAGGATGATA+AGG 0.518535 8.3:+65471299 MS.gene92135:CDS
GGATGAGGCGAGGTTGTTGA+AGG 0.519284 8.3:+65471347 MS.gene92135:CDS
AGAAGGTTTGGATTGCTGCT+GGG 0.519846 8.3:+65471256 MS.gene92135:CDS
CCTTCTTGTGCATCTTTCCA+CGG 0.526928 8.3:-65471239 None:intergenic
ATGCCGAAGAACAAGGGAAA+GGG 0.538255 8.3:+65471057 MS.gene92135:CDS
GATTGATGGGGATGATGATG+AGG 0.545936 8.3:+65471422 MS.gene92135:CDS
TTCAACCATGCCGAAGAACA+AGG 0.546102 8.3:+65471050 None:intergenic
GTTCTTCGTATGCTTGGAAA+CGG 0.546331 8.3:+65471168 MS.gene92135:CDS
CTTGAAGTATATGCCGGATG+AGG 0.557673 8.3:+65471332 MS.gene92135:CDS
ATTGATGGGGATGATGATGA+GGG 0.568262 8.3:+65471423 MS.gene92135:CDS
CTTTGTTCCATCGATACACA+TGG 0.573074 8.3:-65471202 None:intergenic
GGAGGAAAGAATCGCAAGAG+AGG 0.578624 8.3:+65471078 MS.gene92135:CDS
CCGTGGAAAGATGCACAAGA+AGG 0.600822 8.3:+65471239 MS.gene92135:CDS
CATGCCGAAGAACAAGGGAA+AGG 0.602323 8.3:+65471056 None:intergenic
TTAATGAAGGAATTGGTGGT+GGG 0.613970 8.3:+65471400 MS.gene92135:CDS
AAGCGTCTCTGTCATATCCG+TGG 0.616715 8.3:+65471222 MS.gene92135:CDS
GCGTATAGCGAGCTTCCTGA+AGG 0.618073 8.3:+65471369 MS.gene92135:CDS
GAAGGTGTGAGGCTTAATGA+AGG 0.621479 8.3:+65471387 MS.gene92135:CDS
TGTGAAGCCATGTGTATCGA+TGG 0.627821 8.3:+65471195 MS.gene92135:CDS
CCGAAGAACAAGGGAAAGGG+AGG 0.634335 8.3:+65471060 MS.gene92135:CDS
AATTGGTGGTGGGATTGATG+GGG 0.634571 8.3:+65471410 MS.gene92135:CDS
AGGCTTAATGAAGGAATTGG+TGG 0.634691 8.3:+65471396 MS.gene92135:CDS
TCAACCATGCCGAAGAACAA+GGG 0.635918 8.3:+65471051 None:intergenic
GCGAGCTTCCTGAAGGTGTG+AGG 0.643888 8.3:+65471376 MS.gene92135:CDS
AGATGGTCAAGAATACGCAC+AGG 0.647023 8.3:+65471146 MS.gene92135:CDS
TGTGATCTTGAAGTATATGC+CGG 0.651550 8.3:+65471326 MS.gene92135:CDS
AGTATATGCCGGATGAGGCG+AGG 0.665508 8.3:+65471337 MS.gene92135:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATGATTATATTGAGTTTG+AGG + chr8.3:65471452-65471471 MS.gene92135:CDS 25.0%
TTATATTGAGTTTGAGGATG+AGG + chr8.3:65471458-65471477 MS.gene92135:CDS 30.0%
CTGGGGATATTATTCTTGTT+GGG + chr8.3:65471274-65471293 MS.gene92135:CDS 35.0%
TGTGATCTTGAAGTATATGC+CGG + chr8.3:65471326-65471345 MS.gene92135:CDS 35.0%
! TTAATGAAGGAATTGGTGGT+GGG + chr8.3:65471400-65471419 MS.gene92135:CDS 35.0%
AGGCTTAATGAAGGAATTGG+TGG + chr8.3:65471396-65471415 MS.gene92135:CDS 40.0%
CTTAATGAAGGAATTGGTGG+TGG + chr8.3:65471399-65471418 MS.gene92135:CDS 40.0%
CTTTGTTCCATCGATACACA+TGG - chr8.3:65471205-65471224 None:intergenic 40.0%
GAAAGATGCACAAGAAGGTT+TGG + chr8.3:65471244-65471263 MS.gene92135:CDS 40.0%
GCGTGATTATCAGGATGATA+AGG + chr8.3:65471299-65471318 MS.gene92135:CDS 40.0%
GCTGGGGATATTATTCTTGT+TGG + chr8.3:65471273-65471292 MS.gene92135:CDS 40.0%
GTGAGGCTTAATGAAGGAAT+TGG + chr8.3:65471393-65471412 MS.gene92135:CDS 40.0%
GTTCTTCGTATGCTTGGAAA+CGG + chr8.3:65471168-65471187 MS.gene92135:CDS 40.0%
! ATTGATGGGGATGATGATGA+GGG + chr8.3:65471423-65471442 MS.gene92135:CDS 40.0%
!! AAAGCGTGAGCTTGTTTTCA+AGG + chr8.3:65471122-65471141 MS.gene92135:CDS 40.0%
!! GAGCTTGTTTTCAAGGAAGA+TGG + chr8.3:65471129-65471148 MS.gene92135:CDS 40.0%
AAGAAGGTTTGGATTGCTGC+TGG + chr8.3:65471255-65471274 MS.gene92135:CDS 45.0%
AGAAGGTTTGGATTGCTGCT+GGG + chr8.3:65471256-65471275 MS.gene92135:CDS 45.0%
AGATGGTCAAGAATACGCAC+AGG + chr8.3:65471146-65471165 MS.gene92135:CDS 45.0%
ATGCCGAAGAACAAGGGAAA+GGG + chr8.3:65471057-65471076 MS.gene92135:CDS 45.0%
CCTTCTTGTGCATCTTTCCA+CGG - chr8.3:65471242-65471261 None:intergenic 45.0%
CTTGAAGTATATGCCGGATG+AGG + chr8.3:65471332-65471351 MS.gene92135:CDS 45.0%
TCATTAAGCCTCACACCTTC+AGG - chr8.3:65471387-65471406 None:intergenic 45.0%
TGTGAAGCCATGTGTATCGA+TGG + chr8.3:65471195-65471214 MS.gene92135:CDS 45.0%
! AAGCTCACGCTTTTCGTCAT+CGG - chr8.3:65471115-65471134 None:intergenic 45.0%
! GAAGGTGTGAGGCTTAATGA+AGG + chr8.3:65471387-65471406 MS.gene92135:CDS 45.0%
! GATTGATGGGGATGATGATG+AGG + chr8.3:65471422-65471441 MS.gene92135:CDS 45.0%
! TGTTGGGTTGCGTGATTATC+AGG + chr8.3:65471290-65471309 MS.gene92135:CDS 45.0%
!! AATTGGTGGTGGGATTGATG+GGG + chr8.3:65471410-65471429 MS.gene92135:CDS 45.0%
!! GAATTGGTGGTGGGATTGAT+GGG + chr8.3:65471409-65471428 MS.gene92135:CDS 45.0%
AAGCGTCTCTGTCATATCCG+TGG + chr8.3:65471222-65471241 MS.gene92135:CDS 50.0%
CCGTGGAAAGATGCACAAGA+AGG + chr8.3:65471239-65471258 MS.gene92135:CDS 50.0%
CCTCCCTTTCCCTTGTTCTT+CGG - chr8.3:65471063-65471082 None:intergenic 50.0%
GAAGGTTTGGATTGCTGCTG+GGG + chr8.3:65471257-65471276 MS.gene92135:CDS 50.0%
GCACAGGTTCTTCGTATGCT+TGG + chr8.3:65471162-65471181 MS.gene92135:CDS 50.0%
GGAGGAAAGAATCGCAAGAG+AGG + chr8.3:65471078-65471097 MS.gene92135:CDS 50.0%
TTCAACAACCTCGCCTCATC+CGG - chr8.3:65471348-65471367 None:intergenic 50.0%
! GGGATGATGATGAGGGTGAT+GGG + chr8.3:65471430-65471449 MS.gene92135:CDS 50.0%
!! GGAATTGGTGGTGGGATTGA+TGG + chr8.3:65471408-65471427 MS.gene92135:CDS 50.0%
AGTATATGCCGGATGAGGCG+AGG + chr8.3:65471337-65471356 MS.gene92135:CDS 55.0%
CCGAAGAACAAGGGAAAGGG+AGG + chr8.3:65471060-65471079 MS.gene92135:CDS 55.0%
GCGTATAGCGAGCTTCCTGA+AGG + chr8.3:65471369-65471388 MS.gene92135:CDS 55.0%
GGATGAGGCGAGGTTGTTGA+AGG + chr8.3:65471347-65471366 MS.gene92135:CDS 55.0%
! GGGGATGATGATGAGGGTGA+TGG + chr8.3:65471429-65471448 MS.gene92135:CDS 55.0%
! GCGAGCTTCCTGAAGGTGTG+AGG + chr8.3:65471376-65471395 MS.gene92135:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 65471057 65471497 65471057 ID=MS.gene92135
chr8.3 mRNA 65471057 65471497 65471057 ID=MS.gene92135.t1;Parent=MS.gene92135
chr8.3 exon 65471057 65471497 65471057 ID=MS.gene92135.t1.exon1;Parent=MS.gene92135.t1
chr8.3 CDS 65471057 65471497 65471057 ID=cds.MS.gene92135.t1;Parent=MS.gene92135.t1
Gene Sequence

>MS.gene92135

ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGCAAGAGAGGAAAGAACGAAGCCGATGACGAAAAGCGTGAGCTTGTTTTCAAGGAAGATGGTCAAGAATACGCACAGGTTCTTCGTATGCTTGGAAACGGTCGTTGTGAAGCCATGTGTATCGATGGAACAAAGCGTCTCTGTCATATCCGTGGAAAGATGCACAAGAAGGTTTGGATTGCTGCTGGGGATATTATTCTTGTTGGGTTGCGTGATTATCAGGATGATAAGGCTGATGTGATCTTGAAGTATATGCCGGATGAGGCGAGGTTGTTGAAGGCGTATAGCGAGCTTCCTGAAGGTGTGAGGCTTAATGAAGGAATTGGTGGTGGGATTGATGGGGATGATGATGAGGGTGATGGGAATGATTATATTGAGTTTGAGGATGAGGATATTGATAAGATTTGA

Protein sequence

>MS.gene92135.t1

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYSELPEGVRLNEGIGGGIDGDDDEGDGNDYIEFEDEDIDKI