AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029370.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029370.01.T01 MTR_5g084930 98.148 108 2 0 3 110 328 435 1.27e-70 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029370.01.T01 AT2G32920 71.818 110 28 3 3 110 332 440 3.29e-46 154
MsG0580029370.01.T01 AT1G04980 69.369 111 29 3 4 110 338 447 5.17e-45 151

Find 50 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATCAATTGTTCATTAA+TGG 0.149709 5:+96047869 None:intergenic
TACGAGAATACCTGGTTCTT+TGG 0.232463 5:-96047840 MsG0580029370.01.T01:three_prime_UTR
GATAGTATGTAGAATATTAA+AGG 0.283108 5:-96048062 MsG0580029370.01.T01:three_prime_UTR
TCAATCAATTGTTCATTAAT+GGG 0.300305 5:+96047870 None:intergenic
GTTTCCTTATAGTTCATCTT+TGG 0.305542 5:+96048146 None:intergenic
CTCAAGGTCTGGCTGCTTAC+CGG 0.356762 5:+96048526 None:intergenic
AACACCTTTCTTAACGTTCA+AGG 0.373568 5:+96048460 None:intergenic
TCAACCAAAGATGAACTATA+AGG 0.387123 5:-96048150 MsG0580029370.01.T01:exon
AAAGTTGGTGTTGGTGGGTA+TGG 0.390826 5:-96048500 MsG0580029370.01.T01:CDS
CATCTCCTATAGCATAAAAC+TGG 0.409206 5:+96048084 None:intergenic
GGGTCTTGAACAGCTATGTA+TGG 0.429572 5:-96048556 None:intergenic
AGAGAATTTGTGAAAGAAGC+TGG 0.439854 5:-96048309 MsG0580029370.01.T01:CDS
CCAACTTTCTGCTCAAGGTC+TGG 0.450805 5:+96048515 None:intergenic
TTATAGTTCATCTTTGGTTG+AGG 0.465932 5:+96048152 None:intergenic
ATAGTATGTAGAATATTAAA+GGG 0.475068 5:-96048061 MsG0580029370.01.T01:three_prime_UTR
ACGTTCAAGGCTACTAATGC+TGG 0.481617 5:+96048473 None:intergenic
GGTTCTGTCTTTACAATGGT+GGG 0.490337 5:+96048246 None:intergenic
GAACCATGGGATGGAAAAGA+TGG 0.495841 5:-96048228 MsG0580029370.01.T01:CDS
TGGTTCTGTCTTTACAATGG+TGG 0.496152 5:+96048245 None:intergenic
GAAGCTGGACGTGGAGGCAA+AGG 0.515570 5:-96048294 MsG0580029370.01.T01:CDS
TGGTGGGAGGAGTGTCACCT+AGG 0.522614 5:+96048262 None:intergenic
CCAGACCTTGAGCAGAAAGT+TGG 0.535182 5:-96048515 MsG0580029370.01.T01:CDS
GGTGGGAGGAGTGTCACCTA+GGG 0.535529 5:+96048263 None:intergenic
GAGCAGAAAGTTGGTGTTGG+TGG 0.549595 5:-96048506 MsG0580029370.01.T01:CDS
AACATTATTACGAGAATACC+TGG 0.552609 5:-96047848 MsG0580029370.01.T01:three_prime_UTR
CCATGGTTCTGTCTTTACAA+TGG 0.553296 5:+96048242 None:intergenic
GTAGCCTTGAACGTTAAGAA+AGG 0.556407 5:-96048464 MsG0580029370.01.T01:CDS
AACATACCTCAGGAAAATGT+TGG 0.556667 5:-96047914 MsG0580029370.01.T01:three_prime_UTR
AAAAGATGGAGAAATAATTG+AGG 0.562273 5:-96048214 MsG0580029370.01.T01:CDS
CAACACCAACTTTCTGCTCA+AGG 0.577087 5:+96048510 None:intergenic
AGCAGAAAGTTGGTGTTGGT+GGG 0.581032 5:-96048505 MsG0580029370.01.T01:CDS
AAGCTGGACGTGGAGGCAAA+GGG 0.583780 5:-96048293 MsG0580029370.01.T01:CDS
TTTGTGAAAGAAGCTGGACG+TGG 0.587104 5:-96048303 MsG0580029370.01.T01:CDS
AGCTATGTATGGGCAGCTGC+CGG 0.590014 5:-96048545 MsG0580029370.01.T01:CDS
TTCATGTCATTCTCATTCAG+TGG 0.591892 5:-96047940 MsG0580029370.01.T01:three_prime_UTR
GGTCTTGAACAGCTATGTAT+GGG 0.597619 5:-96048555 None:intergenic
TCTGTCTTTACAATGGTGGG+AGG 0.605063 5:+96048249 None:intergenic
AGTTCAAAAGCACTCTTGAG+AGG 0.621890 5:+96048431 None:intergenic
TCAGTGGTACAACATACCTC+AGG 0.622601 5:-96047924 MsG0580029370.01.T01:three_prime_UTR
CATTGTAAAGACAGAACCAT+GGG 0.623172 5:-96048241 MsG0580029370.01.T01:CDS
GGCAAAGGGAACTTGCCCCT+AGG 0.627464 5:-96048279 MsG0580029370.01.T01:CDS
CTTGAGCAGAAAGTTGGTGT+TGG 0.628583 5:-96048509 MsG0580029370.01.T01:CDS
GTAAAGACAGAACCATGGGA+TGG 0.640244 5:-96048237 MsG0580029370.01.T01:CDS
GAGGAACGATCCAAAGAACC+AGG 0.646887 5:+96047830 None:intergenic
GTGAAAGAAGCTGGACGTGG+AGG 0.650513 5:-96048300 MsG0580029370.01.T01:CDS
AAGACTAGTATAGTGATGAG+TGG 0.668123 5:-96047976 MsG0580029370.01.T01:three_prime_UTR
GTGGGAGGAGTGTCACCTAG+GGG 0.673404 5:+96048264 None:intergenic
GAATATTAAAGGGAAATGTG+TGG 0.677310 5:-96048051 MsG0580029370.01.T01:three_prime_UTR
ACAACCGGCTATGGTCAATG+AGG 0.692842 5:+96047811 None:intergenic
CCATTGTAAAGACAGAACCA+TGG 0.708421 5:-96048242 MsG0580029370.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAGACTTACATAATT+TGG + Chr5:96047972-96047991 None:intergenic 15.0%
!! ATAGTATGTAGAATATTAAA+GGG - Chr5:96048313-96048332 MsG0580029370.01.T01:intron 15.0%
!! GATAGTATGTAGAATATTAA+AGG - Chr5:96048312-96048331 MsG0580029370.01.T01:intron 20.0%
!! TCAATCAATTGTTCATTAAT+GGG + Chr5:96048507-96048526 None:intergenic 20.0%
!! TTCAATCAATTGTTCATTAA+TGG + Chr5:96048508-96048527 None:intergenic 20.0%
!!! CTAATTTTATGCTAAATAGT+TGG - Chr5:96047992-96048011 MsG0580029370.01.T01:three_prime_UTR 20.0%
!!! TTTTTCAGATTTTTCTTGTT+CGG - Chr5:96048370-96048389 MsG0580029370.01.T01:intron 20.0%
! AAAAGATGGAGAAATAATTG+AGG - Chr5:96048160-96048179 MsG0580029370.01.T01:CDS 25.0%
AACATTATTACGAGAATACC+TGG - Chr5:96048526-96048545 MsG0580029370.01.T01:CDS 30.0%
GAATATTAAAGGGAAATGTG+TGG - Chr5:96048323-96048342 MsG0580029370.01.T01:intron 30.0%
GTTTCCTTATAGTTCATCTT+TGG + Chr5:96048231-96048250 None:intergenic 30.0%
TCAACCAAAGATGAACTATA+AGG - Chr5:96048224-96048243 MsG0580029370.01.T01:CDS 30.0%
TTATAGTTCATCTTTGGTTG+AGG + Chr5:96048225-96048244 None:intergenic 30.0%
! ATTTTCTCTTGAAGAACTCA+TGG - Chr5:96048190-96048209 MsG0580029370.01.T01:CDS 30.0%
! TTTTCTCTTGAAGAACTCAT+GGG - Chr5:96048191-96048210 MsG0580029370.01.T01:CDS 30.0%
!! CATTACCAGTTTTATGCTAT+AGG - Chr5:96048285-96048304 MsG0580029370.01.T01:CDS 30.0%
AACACCTTTCTTAACGTTCA+AGG + Chr5:96047917-96047936 None:intergenic 35.0%
AACATACCTCAGGAAAATGT+TGG - Chr5:96048460-96048479 MsG0580029370.01.T01:CDS 35.0%
AAGACTAGTATAGTGATGAG+TGG - Chr5:96048398-96048417 MsG0580029370.01.T01:intron 35.0%
AGAGAATTTGTGAAAGAAGC+TGG - Chr5:96048065-96048084 MsG0580029370.01.T01:three_prime_UTR 35.0%
CATCTCCTATAGCATAAAAC+TGG + Chr5:96048293-96048312 None:intergenic 35.0%
CATTGTAAAGACAGAACCAT+GGG - Chr5:96048133-96048152 MsG0580029370.01.T01:exon 35.0%
GAAAAATCTGAAAAACAGCG+AGG + Chr5:96048365-96048384 None:intergenic 35.0%
TTCATGTCATTCTCATTCAG+TGG - Chr5:96048434-96048453 MsG0580029370.01.T01:CDS 35.0%
CCATTGTAAAGACAGAACCA+TGG - Chr5:96048132-96048151 MsG0580029370.01.T01:three_prime_UTR 40.0%
GGTTCTGTCTTTACAATGGT+GGG + Chr5:96048131-96048150 None:intergenic 40.0%
! AACATGCCAACATTTTCCTG+AGG + Chr5:96048469-96048488 None:intergenic 40.0%
! CCATGGTTCTGTCTTTACAA+TGG + Chr5:96048135-96048154 None:intergenic 40.0%
! GTAGCCTTGAACGTTAAGAA+AGG - Chr5:96047910-96047929 MsG0580029370.01.T01:three_prime_UTR 40.0%
! TACGAGAATACCTGGTTCTT+TGG - Chr5:96048534-96048553 MsG0580029370.01.T01:CDS 40.0%
! TAGTGATGAGTGGTCTTTTG+AGG - Chr5:96048408-96048427 MsG0580029370.01.T01:intron 40.0%
! TGGTTCTGTCTTTACAATGG+TGG + Chr5:96048132-96048151 None:intergenic 40.0%
!! AGTTCAAAAGCACTCTTGAG+AGG + Chr5:96047946-96047965 None:intergenic 40.0%
ACGTTCAAGGCTACTAATGC+TGG + Chr5:96047904-96047923 None:intergenic 45.0%
CAACACCAACTTTCTGCTCA+AGG + Chr5:96047867-96047886 None:intergenic 45.0%
GAACCATGGGATGGAAAAGA+TGG - Chr5:96048146-96048165 MsG0580029370.01.T01:exon 45.0%
GTAAAGACAGAACCATGGGA+TGG - Chr5:96048137-96048156 MsG0580029370.01.T01:exon 45.0%
TCAGTGGTACAACATACCTC+AGG - Chr5:96048450-96048469 MsG0580029370.01.T01:CDS 45.0%
TGCCCATACATAGCTGTTCA+AGG + Chr5:96047824-96047843 None:intergenic 45.0%
TTTGTGAAAGAAGCTGGACG+TGG - Chr5:96048071-96048090 MsG0580029370.01.T01:three_prime_UTR 45.0%
! TCTCCATCTTTTCCATCCCA+TGG + Chr5:96048152-96048171 None:intergenic 45.0%
! TCTGTCTTTACAATGGTGGG+AGG + Chr5:96048128-96048147 None:intergenic 45.0%
!! AAAGTTGGTGTTGGTGGGTA+TGG - Chr5:96047874-96047893 MsG0580029370.01.T01:three_prime_UTR 45.0%
!! AGCAGAAAGTTGGTGTTGGT+GGG - Chr5:96047869-96047888 MsG0580029370.01.T01:three_prime_UTR 45.0%
!! CTTGAGCAGAAAGTTGGTGT+TGG - Chr5:96047865-96047884 MsG0580029370.01.T01:three_prime_UTR 45.0%
CCAACTTTCTGCTCAAGGTC+TGG + Chr5:96047862-96047881 None:intergenic 50.0%
CCAGACCTTGAGCAGAAAGT+TGG - Chr5:96047859-96047878 MsG0580029370.01.T01:three_prime_UTR 50.0%
GAGGAACGATCCAAAGAACC+AGG + Chr5:96048547-96048566 None:intergenic 50.0%
!! GAGCAGAAAGTTGGTGTTGG+TGG - Chr5:96047868-96047887 MsG0580029370.01.T01:three_prime_UTR 50.0%
AAGCTGGACGTGGAGGCAAA+GGG - Chr5:96048081-96048100 MsG0580029370.01.T01:three_prime_UTR 55.0%
AGCTATGTATGGGCAGCTGC+CGG - Chr5:96047829-96047848 MsG0580029370.01.T01:three_prime_UTR 55.0%
CTCAAGGTCTGGCTGCTTAC+CGG + Chr5:96047851-96047870 None:intergenic 55.0%
GTGAAAGAAGCTGGACGTGG+AGG - Chr5:96048074-96048093 MsG0580029370.01.T01:three_prime_UTR 55.0%
GAAGCTGGACGTGGAGGCAA+AGG - Chr5:96048080-96048099 MsG0580029370.01.T01:three_prime_UTR 60.0%
GGCAAAGGGAACTTGCCCCT+AGG - Chr5:96048095-96048114 MsG0580029370.01.T01:three_prime_UTR 60.0%
!! GGTGGGAGGAGTGTCACCTA+GGG + Chr5:96048114-96048133 None:intergenic 60.0%
!! GTGGGAGGAGTGTCACCTAG+GGG + Chr5:96048113-96048132 None:intergenic 60.0%
!! TGGTGGGAGGAGTGTCACCT+AGG + Chr5:96048115-96048134 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 96047823 96048573 96047823 ID=MsG0580029370.01;Name=MsG0580029370.01
Chr5 mRNA 96047823 96048573 96047823 ID=MsG0580029370.01.T01;Parent=MsG0580029370.01;Name=MsG0580029370.01.T01;_AED=0.50;_eAED=0.50;_QI=0|1|0.5|1|1|1|2|329|110
Chr5 exon 96047823 96048329 96047823 ID=MsG0580029370.01.T01:exon:36113;Parent=MsG0580029370.01.T01
Chr5 exon 96048419 96048573 96048419 ID=MsG0580029370.01.T01:exon:36112;Parent=MsG0580029370.01.T01
Chr5 CDS 96048419 96048573 96048419 ID=MsG0580029370.01.T01:cds;Parent=MsG0580029370.01.T01
Chr5 CDS 96048152 96048329 96048152 ID=MsG0580029370.01.T01:cds;Parent=MsG0580029370.01.T01
Chr5 three_prime_UTR 96047823 96048151 96047823 ID=MsG0580029370.01.T01:three_prime_utr;Parent=MsG0580029370.01.T01
Gene Sequence

>MsG0580029370.01.T01

TTGAACAGCTATGTATGGGCAGCTGCCGGTAAGCAGCCAGACCTTGAGCAGAAAGTTGGTGTTGGTGGGTATGGTTATCCAGCATTAGTAGCCTTGAACGTTAAGAAAGGTGTTTATGCTCCTCTCAAGAGTGCTTTTGAACTTGACCAAATTATAGAATTTGTGAAAGAAGCTGGACGTGGAGGCAAAGGGAACTTGCCCCTAGGTGACACTCCTCCCACCATTGTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAAGATGAATTTTCTCTTGAAGAACTCATGGGAGATGATGCCTCAACCAAAGATGAACTATAA

Protein sequence

>MsG0580029370.01.T01

LNSYVWAAAGKQPDLEQKVGVGGYGYPALVALNVKKGVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLGDTPPTIVKTEPWDGKDGEIIEEDEFSLEELMGDDASTKDEL*