AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038321.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038321.01.T01 MTR_7g051210 100.000 172 0 0 1 172 25 196 9.00e-121 339
MsG0780038321.01.T01 MTR_3g069580 48.538 171 81 3 3 171 4 169 2.08e-38 129
MsG0780038321.01.T01 MTR_4g064923 42.778 180 89 2 5 172 10 187 7.63e-34 118
MsG0780038321.01.T01 MTR_7g005460 75.510 49 12 0 117 165 1 49 9.98e-19 76.6
MsG0780038321.01.T01 MTR_6g092500 79.545 44 9 0 117 160 1 44 1.19e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038321.01.T01 AT3G07510 85.366 164 24 0 9 172 26 189 2.06e-88 257
MsG0780038321.01.T01 AT3G07510 85.366 164 24 0 9 172 26 189 2.06e-88 257
MsG0780038321.01.T01 AT3G07510 85.366 164 24 0 9 172 36 199 2.22e-88 257
MsG0780038321.01.T01 AT2G01580 61.006 159 62 0 12 170 31 189 3.82e-59 183
MsG0780038321.01.T01 AT5G05950 47.126 174 85 4 6 172 2 175 6.96e-43 141
MsG0780038321.01.T01 AT3G46890 40.000 190 90 7 5 172 17 204 7.91e-25 95.9

Find 34 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGATGTTGGGATCTGCTT+TGG 0.256332 7:-44187315 MsG0780038321.01.T01:CDS
TCTGCTTTGGGATCTGTAAT+GGG 0.285528 7:-44187302 MsG0780038321.01.T01:CDS
GGGATGTTGGGATCTGCTTT+GGG 0.300771 7:-44187314 MsG0780038321.01.T01:CDS
TGCTCATGCTGTTGCTGTTA+TGG 0.304958 7:-44187201 MsG0780038321.01.T01:CDS
TTCTCCAGGCTGCGTTGGCC+TGG 0.319684 7:+44187524 None:intergenic
ATCTGCTTTGGGATCTGTAA+TGG 0.352823 7:-44187303 MsG0780038321.01.T01:CDS
AATGTTATTGAGATTAGGTT+GGG 0.375138 7:-44187251 MsG0780038321.01.T01:CDS
CGAATACGGCGATTGTGAAA+AGG 0.429453 7:+44187567 None:intergenic
TCATCTTCCCTTGTTGCTCA+AGG 0.433862 7:-44187422 MsG0780038321.01.T01:CDS
TAGAGATAGGCCGACGAATA+CGG 0.437603 7:+44187553 None:intergenic
GAATACGGCGATTGTGAAAA+GGG 0.443002 7:+44187568 None:intergenic
AACTCCAGGCCAACGCAGCC+TGG 0.450154 7:-44187528 MsG0780038321.01.T01:CDS
CTTCTTTGCGACGTCGATGC+CGG 0.473260 7:+44187484 None:intergenic
GAATGTTATTGAGATTAGGT+TGG 0.491944 7:-44187252 MsG0780038321.01.T01:CDS
TGGTGAATGTTATTGAGATT+AGG 0.494523 7:-44187256 MsG0780038321.01.T01:CDS
TCGTGCACATATTAATCTGA+AGG 0.516683 7:-44187348 MsG0780038321.01.T01:CDS
TTCACAATCGCCGTATTCGT+CGG 0.523383 7:-44187563 MsG0780038321.01.T01:CDS
CGCAAGAGGAGCGATTCTCC+AGG 0.526259 7:+44187510 None:intergenic
GGCCTGGAGTTGTTAGAGAT+AGG 0.531373 7:+44187540 None:intergenic
AATCGCTCCTCTTGCGACGC+CGG 0.533788 7:-44187503 MsG0780038321.01.T01:CDS
TCTTCTCAACAGTAAGGATG+TGG 0.538914 7:-44187381 MsG0780038321.01.T01:CDS
GGCCTATCTCTAACAACTCC+AGG 0.544683 7:-44187542 MsG0780038321.01.T01:CDS
AGCGATTCTCCAGGCTGCGT+TGG 0.549775 7:+44187519 None:intergenic
GTCGATGCCGGCGTCGCAAG+AGG 0.558642 7:+44187496 None:intergenic
TTTAAGACCTTGAGCAACAA+GGG 0.559838 7:+44187415 None:intergenic
ACTCAATCTTCTCAACAGTA+AGG 0.560977 7:-44187387 MsG0780038321.01.T01:CDS
CTGCTTTGGGATCTGTAATG+GGG 0.592904 7:-44187301 MsG0780038321.01.T01:CDS
AGGTTGGGGATGTTGTCTTG+TGG 0.593579 7:-44187236 MsG0780038321.01.T01:CDS
GTTTAAGACCTTGAGCAACA+AGG 0.593709 7:+44187414 None:intergenic
AGATGAGAGAAAGAAGCTGA+AGG 0.598891 7:+44187439 None:intergenic
ATGTTATTGAGATTAGGTTG+GGG 0.603102 7:-44187250 MsG0780038321.01.T01:CDS
GTACACGTCACCGCATTAGA+CGG 0.618139 7:-44187608 MsG0780038321.01.T01:CDS
GTTGACTAGTCCGTCTAATG+CGG 0.635569 7:+44187598 None:intergenic
GTGTACGCTTGTTGTTGATG+AGG 0.652825 7:+44187625 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATGTTATTGAGATTAGGTT+GGG - Chr7:44187517-44187536 MsG0780038321.01.T01:CDS 25.0%
GAATGTTATTGAGATTAGGT+TGG - Chr7:44187516-44187535 MsG0780038321.01.T01:CDS 30.0%
TGGTGAATGTTATTGAGATT+AGG - Chr7:44187512-44187531 MsG0780038321.01.T01:CDS 30.0%
! ATGTTATTGAGATTAGGTTG+GGG - Chr7:44187518-44187537 MsG0780038321.01.T01:CDS 30.0%
!!! TAATCTGAAGGTTTTGAGAT+GGG - Chr7:44187432-44187451 MsG0780038321.01.T01:CDS 30.0%
!!! TTAATCTGAAGGTTTTGAGA+TGG - Chr7:44187431-44187450 MsG0780038321.01.T01:CDS 30.0%
!!! TTTGTTTTTGATGCTTTCGA+TGG - Chr7:44187492-44187511 MsG0780038321.01.T01:CDS 30.0%
ACTCAATCTTCTCAACAGTA+AGG - Chr7:44187381-44187400 MsG0780038321.01.T01:CDS 35.0%
TCGTGCACATATTAATCTGA+AGG - Chr7:44187420-44187439 MsG0780038321.01.T01:CDS 35.0%
! TTTAAGACCTTGAGCAACAA+GGG + Chr7:44187356-44187375 None:intergenic 35.0%
!!! AATCTGAAGGTTTTGAGATG+GGG - Chr7:44187433-44187452 MsG0780038321.01.T01:CDS 35.0%
AGATGAGAGAAAGAAGCTGA+AGG + Chr7:44187332-44187351 None:intergenic 40.0%
GAATACGGCGATTGTGAAAA+GGG + Chr7:44187203-44187222 None:intergenic 40.0%
TCTTCTCAACAGTAAGGATG+TGG - Chr7:44187387-44187406 MsG0780038321.01.T01:CDS 40.0%
! GTTTAAGACCTTGAGCAACA+AGG + Chr7:44187357-44187376 None:intergenic 40.0%
!! ATCTGCTTTGGGATCTGTAA+TGG - Chr7:44187465-44187484 MsG0780038321.01.T01:CDS 40.0%
!! TCTGCTTTGGGATCTGTAAT+GGG - Chr7:44187466-44187485 MsG0780038321.01.T01:CDS 40.0%
!!! ATCTGAAGGTTTTGAGATGG+GGG - Chr7:44187434-44187453 MsG0780038321.01.T01:CDS 40.0%
CGAATACGGCGATTGTGAAA+AGG + Chr7:44187204-44187223 None:intergenic 45.0%
TAGAGATAGGCCGACGAATA+CGG + Chr7:44187218-44187237 None:intergenic 45.0%
TCATCTTCCCTTGTTGCTCA+AGG - Chr7:44187346-44187365 MsG0780038321.01.T01:CDS 45.0%
TTCACAATCGCCGTATTCGT+CGG - Chr7:44187205-44187224 MsG0780038321.01.T01:CDS 45.0%
! GTTGACTAGTCCGTCTAATG+CGG + Chr7:44187173-44187192 None:intergenic 45.0%
! TGCTCATGCTGTTGCTGTTA+TGG - Chr7:44187567-44187586 MsG0780038321.01.T01:CDS 45.0%
!! CTGCTTTGGGATCTGTAATG+GGG - Chr7:44187467-44187486 MsG0780038321.01.T01:CDS 45.0%
!! GTGTACGCTTGTTGTTGATG+AGG + Chr7:44187146-44187165 None:intergenic 45.0%
!!! GTTTTGAGATGGGGGATGTT+GGG - Chr7:44187442-44187461 MsG0780038321.01.T01:CDS 45.0%
AGGTTGGGGATGTTGTCTTG+TGG - Chr7:44187532-44187551 MsG0780038321.01.T01:CDS 50.0%
GGCCTATCTCTAACAACTCC+AGG - Chr7:44187226-44187245 MsG0780038321.01.T01:CDS 50.0%
GGCCTGGAGTTGTTAGAGAT+AGG + Chr7:44187231-44187250 None:intergenic 50.0%
GGGATGTTGGGATCTGCTTT+GGG - Chr7:44187454-44187473 MsG0780038321.01.T01:CDS 50.0%
GTACACGTCACCGCATTAGA+CGG - Chr7:44187160-44187179 MsG0780038321.01.T01:CDS 50.0%
!!! GGTTTTGAGATGGGGGATGT+TGG - Chr7:44187441-44187460 MsG0780038321.01.T01:CDS 50.0%
CTTCTTTGCGACGTCGATGC+CGG + Chr7:44187287-44187306 None:intergenic 55.0%
GGGGATGTTGGGATCTGCTT+TGG - Chr7:44187453-44187472 MsG0780038321.01.T01:CDS 55.0%
AATCGCTCCTCTTGCGACGC+CGG - Chr7:44187265-44187284 MsG0780038321.01.T01:CDS 60.0%
AGCGATTCTCCAGGCTGCGT+TGG + Chr7:44187252-44187271 None:intergenic 60.0%
CGCAAGAGGAGCGATTCTCC+AGG + Chr7:44187261-44187280 None:intergenic 60.0%
AACTCCAGGCCAACGCAGCC+TGG - Chr7:44187240-44187259 MsG0780038321.01.T01:CDS 65.0%
! TTCTCCAGGCTGCGTTGGCC+TGG + Chr7:44187247-44187266 None:intergenic 65.0%
GTCGATGCCGGCGTCGCAAG+AGG + Chr7:44187275-44187294 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 44187136 44187654 44187136 ID=MsG0780038321.01;Name=MsG0780038321.01
Chr7 mRNA 44187136 44187654 44187136 ID=MsG0780038321.01.T01;Parent=MsG0780038321.01;Name=MsG0780038321.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|172
Chr7 exon 44187136 44187654 44187136 ID=MsG0780038321.01.T01:exon:4806;Parent=MsG0780038321.01.T01
Chr7 CDS 44187136 44187654 44187136 ID=MsG0780038321.01.T01:cds;Parent=MsG0780038321.01.T01
Gene Sequence

>MsG0780038321.01.T01

ATGTCATCCTCATCAACAACAAGCGTACACGTCACCGCATTAGACGGACTAGTCAACGTAAACTCCCTTTTCACAATCGCCGTATTCGTCGGCCTATCTCTAACAACTCCAGGCCAACGCAGCCTGGAGAATCGCTCCTCTTGCGACGCCGGCATCGACGTCGCAAAGAAGCTTCTAGTCTTCGAAGTTGTTTCCTTCAGCTTCTTTCTCTCATCTTCCCTTGTTGCTCAAGGTCTTAAACTTGCACTCAATCTTCTCAACAGTAAGGATGTGGATGAAGCTTTTCGTGCACATATTAATCTGAAGGTTTTGAGATGGGGGATGTTGGGATCTGCTTTGGGATCTGTAATGGGGTGTTTGTTTTTGATGCTTTCGATGGTGAATGTTATTGAGATTAGGTTGGGGATGTTGTCTTGTGGAAGTAAATCTGCTGCTCATGCTGTTGCTGTTATGGCTGTTCTTGTTACTTCTGCTCTTGTTGTTTATATTTCTACTGCTATTTATGCTTTTACTCACTAG

Protein sequence

>MsG0780038321.01.T01

MSSSSTTSVHVTALDGLVNVNSLFTIAVFVGLSLTTPGQRSLENRSSCDAGIDVAKKLLVFEVVSFSFFLSSSLVAQGLKLALNLLNSKDVDEAFRAHINLKVLRWGMLGSALGSVMGCLFLMLSMVNVIEIRLGMLSCGSKSAAHAVAVMAVLVTSALVVYISTAIYAFTH*