AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0078430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078430 MtrunA17_Chr1g0188931 98.684 76 1 0 4 79 31 106 4.04e-53 160
Msa0078430 MtrunA17_Chr3g0127211 74.667 75 19 0 4 78 31 105 1.19e-38 124
Msa0078430 MtrunA17_Chr8g0346501 68.056 72 23 0 4 75 31 102 1.96e-33 110
Msa0078430 MtrunA17_Chr1g0188951 65.079 63 22 0 17 79 1 63 4.91e-27 93.2
Msa0078430 MtrunA17_Chr2g0316921 48.611 72 37 0 6 77 37 108 5.77e-25 90.1
Msa0078430 MtrunA17_Chr8g0360291 48.571 70 36 0 8 77 39 108 9.61e-24 87.0
Msa0078430 MtrunA17_Chr4g0046851 47.143 70 37 0 8 77 39 108 6.70e-23 84.7
Msa0078430 MtrunA17_Chr5g0436971 46.479 71 38 0 4 74 35 105 5.41e-22 82.4
Msa0078430 MtrunA17_Chr2g0316891 46.154 65 35 0 13 77 42 106 2.31e-21 80.9
Msa0078430 MtrunA17_Chr3g0113291 45.070 71 39 0 4 74 58 128 2.63e-21 81.3
Msa0078430 MtrunA17_Chr7g0242231 48.571 70 36 0 6 75 37 106 2.98e-21 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078430 AT4G27745.2 67.568 74 24 0 4 77 31 104 1.72e-36 119
Msa0078430 AT4G27745.3 67.568 74 24 0 4 77 31 104 1.72e-36 119
Msa0078430 AT4G27745.1 67.568 74 24 0 4 77 31 104 1.72e-36 119
Msa0078430 AT4G27745.4 67.568 74 24 0 4 77 31 104 1.72e-36 119
Msa0078430 AT5G53940.1 45.833 72 39 0 6 77 37 108 4.76e-24 88.2
Msa0078430 AT4G27740.1 44.444 72 40 0 5 76 32 103 3.98e-22 82.4
Msa0078430 AT3G11230.1 49.296 71 36 0 4 74 35 105 5.75e-22 82.8
Msa0078430 AT3G08990.2 47.143 70 37 0 6 75 37 106 7.86e-22 82.4
Msa0078430 AT3G08990.1 47.143 70 37 0 6 75 37 106 7.86e-22 82.4
Msa0078430 AT3G08990.3 47.143 70 37 0 6 75 37 106 7.86e-22 82.4
Msa0078430 AT3G11230.2 49.296 71 36 0 4 74 68 138 1.22e-21 82.8
Msa0078430 AT3G11230.4 50.000 68 34 0 4 71 35 102 1.50e-21 80.9
Msa0078430 AT3G11230.3 50.000 68 34 0 4 71 68 135 3.59e-21 80.9
Msa0078430 AT3G55890.4 47.887 71 37 0 4 74 35 105 4.09e-21 80.5
Msa0078430 AT3G55890.1 47.887 71 37 0 4 74 35 105 4.09e-21 80.5
Msa0078430 AT3G55890.3 47.761 67 35 0 4 70 35 101 1.17e-20 78.6
Msa0078430 AT3G55890.2 47.761 67 35 0 4 70 35 101 1.17e-20 78.6
Msa0078430 AT2G40110.1 46.377 69 37 0 6 74 37 105 3.77e-20 78.2
Msa0078430 AT2G40110.2 45.455 66 36 0 6 71 37 102 2.44e-19 75.5

Find 18 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAAGTCTAAAATTGTT+AGG 0.250834 1_2:-55954809 Msa0078430:CDS
ATGAATTCCATGTCTACTTT+TGG 0.252538 1_2:+55954948 None:intergenic
ATATAATTTCAGGGAAGAAA+TGG 0.360217 1_2:-55955014 Msa0078430:intron
CATGCAATGAATGTCACAAT+AGG 0.409276 1_2:-55954972 Msa0078430:CDS
TGAGAAGTCTAAAATTGTTA+GGG 0.424108 1_2:-55954808 Msa0078430:CDS
AGTAAACATCAGCAATTGTA+TGG 0.440083 1_2:+55954916 None:intergenic
ATGCAATGAATGTCACAATA+GGG 0.486721 1_2:-55954971 Msa0078430:CDS
TGTGACTGCAATCAAGTGCT+TGG 0.515226 1_2:-55954891 Msa0078430:CDS
ACTGCAATCAAGTGCTTGGT+TGG 0.543372 1_2:-55954887 Msa0078430:CDS
TTTCAGGGAAGAAATGGAAG+AGG 0.547363 1_2:-55955008 Msa0078430:intron
GAAGTTTGAGAGAGTCTATG+AGG 0.548405 1_2:-55954865 Msa0078430:CDS
GGATATTTGCTTGTTGTGTA+CGG 0.556397 1_2:+55955780 None:intergenic
GTAGACATGGAATTCATGAC+AGG 0.597395 1_2:-55954942 Msa0078430:CDS
TGAGGAAACTCAAAAGTACA+AGG 0.598867 1_2:-55954847 Msa0078430:CDS
AATAGGGCCAAAAGTAGACA+TGG 0.602332 1_2:-55954955 Msa0078430:CDS
GAAACTCAAAAGTACAAGGA+AGG 0.606538 1_2:-55954843 Msa0078430:CDS
TGAAGACAATCAATCATCAA+CGG 0.627656 1_2:-55955736 Msa0078430:five_prime_UTR
CAACGGCTACATGAACAAGC+AGG 0.647684 1_2:-55955719 Msa0078430:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTGAAATTGAATTAT+TGG + chr1_2:55954899-55954918 None:intergenic 10.0%
!! AAATTATATATAAATGAACT+TGG + chr1_2:55955565-55955584 None:intergenic 10.0%
!! TTCATTTATATATAATTTCA+GGG - chr1_2:55955567-55955586 Msa0078430:intron 10.0%
!!! TAAATTTGAAATTGAATTAT+TGG + chr1_2:55954899-55954918 None:intergenic 10.0%
!! AAATTATATATAAATGAACT+TGG + chr1_2:55955565-55955584 None:intergenic 10.0%
!! TTCATTTATATATAATTTCA+GGG - chr1_2:55955567-55955586 Msa0078430:intron 10.0%
!!! GTATCAAAAATTCTTTAAAA+TGG + chr1_2:55955282-55955301 None:intergenic 15.0%
!!! TATAAGAACGTTATATATTT+TGG - chr1_2:55955487-55955506 Msa0078430:intron 15.0%
!! CAATTTATAATAACATGTTT+AGG - chr1_2:55955523-55955542 Msa0078430:intron 15.0%
!! GTTCATTTATATATAATTTC+AGG - chr1_2:55955566-55955585 Msa0078430:intron 15.0%
!!! GTATCAAAAATTCTTTAAAA+TGG + chr1_2:55955282-55955301 None:intergenic 15.0%
!!! TATAAGAACGTTATATATTT+TGG - chr1_2:55955487-55955506 Msa0078430:intron 15.0%
!! CAATTTATAATAACATGTTT+AGG - chr1_2:55955523-55955542 Msa0078430:intron 15.0%
!! GTTCATTTATATATAATTTC+AGG - chr1_2:55955566-55955585 Msa0078430:intron 15.0%
!! TGAAATAATATCATCATGAA+GGG + chr1_2:55955115-55955134 None:intergenic 20.0%
!! TTGAAATAATATCATCATGA+AGG + chr1_2:55955116-55955135 None:intergenic 20.0%
!! TATTATTTCAAAAACCTTTC+AGG - chr1_2:55955125-55955144 Msa0078430:intron 20.0%
!! AAAGAATACAAATCAAATGA+AGG + chr1_2:55955224-55955243 None:intergenic 20.0%
!!! ATACAATGAAAGTATGAAAA+TGG + chr1_2:55955308-55955327 None:intergenic 20.0%
!! TGAAATAATATCATCATGAA+GGG + chr1_2:55955115-55955134 None:intergenic 20.0%
!! TTGAAATAATATCATCATGA+AGG + chr1_2:55955116-55955135 None:intergenic 20.0%
!! TATTATTTCAAAAACCTTTC+AGG - chr1_2:55955125-55955144 Msa0078430:intron 20.0%
!! AAAGAATACAAATCAAATGA+AGG + chr1_2:55955224-55955243 None:intergenic 20.0%
!!! ATACAATGAAAGTATGAAAA+TGG + chr1_2:55955308-55955327 None:intergenic 20.0%
!!! ATTATGCAAGTTTTGTTTTG+AGG + chr1_2:55954834-55954853 None:intergenic 25.0%
! TTCCAAATCTTAATAGAGAA+AGG + chr1_2:55955165-55955184 None:intergenic 25.0%
! TCACAAAAGTTGTCTAAAAA+TGG - chr1_2:55955383-55955402 Msa0078430:intron 25.0%
! ATATAATTTCAGGGAAGAAA+TGG - chr1_2:55955576-55955595 Msa0078430:intron 25.0%
!! TTGAGAAGTCTAAAATTGTT+AGG - chr1_2:55955781-55955800 Msa0078430:five_prime_UTR 25.0%
!! TGAGAAGTCTAAAATTGTTA+GGG - chr1_2:55955782-55955801 Msa0078430:five_prime_UTR 25.0%
!!! ATTATGCAAGTTTTGTTTTG+AGG + chr1_2:55954834-55954853 None:intergenic 25.0%
! TTCCAAATCTTAATAGAGAA+AGG + chr1_2:55955165-55955184 None:intergenic 25.0%
! TCACAAAAGTTGTCTAAAAA+TGG - chr1_2:55955383-55955402 Msa0078430:intron 25.0%
! ATATAATTTCAGGGAAGAAA+TGG - chr1_2:55955576-55955595 Msa0078430:intron 25.0%
!! TTGAGAAGTCTAAAATTGTT+AGG - chr1_2:55955781-55955800 Msa0078430:five_prime_UTR 25.0%
!! TGAGAAGTCTAAAATTGTTA+GGG - chr1_2:55955782-55955801 Msa0078430:five_prime_UTR 25.0%
TGAAGACAATCAATCATCAA+CGG - chr1_2:55954854-55954873 Msa0078430:CDS 30.0%
ATATGATGATTATCTGCGTT+TGG - chr1_2:55954968-55954987 Msa0078430:CDS 30.0%
!! TTTTATAGCCACTTTGTGAA+GGG - chr1_2:55955009-55955028 Msa0078430:intron 30.0%
!! AGCTCATAAAAGCTTTGAAA+TGG - chr1_2:55955035-55955054 Msa0078430:intron 30.0%
! TTTGAAATGGAGAGTTTGAT+TGG - chr1_2:55955048-55955067 Msa0078430:intron 30.0%
CACCTTTCTCTATTAAGATT+TGG - chr1_2:55955160-55955179 Msa0078430:intron 30.0%
ATCAACACAGTATAAACATG+TGG - chr1_2:55955194-55955213 Msa0078430:intron 30.0%
! GCACATGTGTTAGAATTTTT+GGG + chr1_2:55955458-55955477 None:intergenic 30.0%
! TGCACATGTGTTAGAATTTT+TGG + chr1_2:55955459-55955478 None:intergenic 30.0%
ATGCAATGAATGTCACAATA+GGG - chr1_2:55955619-55955638 Msa0078430:intron 30.0%
! ATGAATTCCATGTCTACTTT+TGG + chr1_2:55955645-55955664 None:intergenic 30.0%
AGTAAACATCAGCAATTGTA+TGG + chr1_2:55955677-55955696 None:intergenic 30.0%
TGAAGACAATCAATCATCAA+CGG - chr1_2:55954854-55954873 Msa0078430:CDS 30.0%
ATATGATGATTATCTGCGTT+TGG - chr1_2:55954968-55954987 Msa0078430:CDS 30.0%
!! TTTTATAGCCACTTTGTGAA+GGG - chr1_2:55955009-55955028 Msa0078430:intron 30.0%
!! AGCTCATAAAAGCTTTGAAA+TGG - chr1_2:55955035-55955054 Msa0078430:intron 30.0%
! TTTGAAATGGAGAGTTTGAT+TGG - chr1_2:55955048-55955067 Msa0078430:intron 30.0%
CACCTTTCTCTATTAAGATT+TGG - chr1_2:55955160-55955179 Msa0078430:intron 30.0%
ATCAACACAGTATAAACATG+TGG - chr1_2:55955194-55955213 Msa0078430:intron 30.0%
! GCACATGTGTTAGAATTTTT+GGG + chr1_2:55955458-55955477 None:intergenic 30.0%
! TGCACATGTGTTAGAATTTT+TGG + chr1_2:55955459-55955478 None:intergenic 30.0%
ATGCAATGAATGTCACAATA+GGG - chr1_2:55955619-55955638 Msa0078430:intron 30.0%
! ATGAATTCCATGTCTACTTT+TGG + chr1_2:55955645-55955664 None:intergenic 30.0%
AGTAAACATCAGCAATTGTA+TGG + chr1_2:55955677-55955696 None:intergenic 30.0%
!! GGATATTTGCTTGTTGTGTA+CGG + chr1_2:55954813-55954832 None:intergenic 35.0%
TCACAAAGTGGCTATAAAAC+TGG + chr1_2:55955008-55955027 None:intergenic 35.0%
!! GTTTTATAGCCACTTTGTGA+AGG - chr1_2:55955008-55955027 Msa0078430:intron 35.0%
CATGCAATGAATGTCACAAT+AGG - chr1_2:55955618-55955637 Msa0078430:intron 35.0%
! TGAGGAAACTCAAAAGTACA+AGG - chr1_2:55955743-55955762 Msa0078430:five_prime_UTR 35.0%
! GAAACTCAAAAGTACAAGGA+AGG - chr1_2:55955747-55955766 Msa0078430:five_prime_UTR 35.0%
ATTGTTAGGGAAAGCTTGTA+AGG - chr1_2:55955795-55955814 Msa0078430:five_prime_UTR 35.0%
!! GGATATTTGCTTGTTGTGTA+CGG + chr1_2:55954813-55954832 None:intergenic 35.0%
TCACAAAGTGGCTATAAAAC+TGG + chr1_2:55955008-55955027 None:intergenic 35.0%
!! GTTTTATAGCCACTTTGTGA+AGG - chr1_2:55955008-55955027 Msa0078430:intron 35.0%
CATGCAATGAATGTCACAAT+AGG - chr1_2:55955618-55955637 Msa0078430:intron 35.0%
! TGAGGAAACTCAAAAGTACA+AGG - chr1_2:55955743-55955762 Msa0078430:five_prime_UTR 35.0%
! GAAACTCAAAAGTACAAGGA+AGG - chr1_2:55955747-55955766 Msa0078430:five_prime_UTR 35.0%
TATCATCATGAAGGGCAACA+TGG + chr1_2:55955107-55955126 None:intergenic 40.0%
TTTCAGGGAAGAAATGGAAG+AGG - chr1_2:55955582-55955601 Msa0078430:intron 40.0%
AATAGGGCCAAAAGTAGACA+TGG - chr1_2:55955635-55955654 Msa0078430:intron 40.0%
GTAGACATGGAATTCATGAC+AGG - chr1_2:55955648-55955667 Msa0078430:intron 40.0%
GAAGTTTGAGAGAGTCTATG+AGG - chr1_2:55955725-55955744 Msa0078430:exon 40.0%
TATCATCATGAAGGGCAACA+TGG + chr1_2:55955107-55955126 None:intergenic 40.0%
TTTCAGGGAAGAAATGGAAG+AGG - chr1_2:55955582-55955601 Msa0078430:intron 40.0%
AATAGGGCCAAAAGTAGACA+TGG - chr1_2:55955635-55955654 Msa0078430:intron 40.0%
GTAGACATGGAATTCATGAC+AGG - chr1_2:55955648-55955667 Msa0078430:intron 40.0%
GAAGTTTGAGAGAGTCTATG+AGG - chr1_2:55955725-55955744 Msa0078430:exon 40.0%
!! TGAGAGAGTGTTCACCTGAA+AGG + chr1_2:55955142-55955161 None:intergenic 45.0%
TGTGACTGCAATCAAGTGCT+TGG - chr1_2:55955699-55955718 Msa0078430:intron 45.0%
ACTGCAATCAAGTGCTTGGT+TGG - chr1_2:55955703-55955722 Msa0078430:intron 45.0%
!! TGAGAGAGTGTTCACCTGAA+AGG + chr1_2:55955142-55955161 None:intergenic 45.0%
TGTGACTGCAATCAAGTGCT+TGG - chr1_2:55955699-55955718 Msa0078430:intron 45.0%
ACTGCAATCAAGTGCTTGGT+TGG - chr1_2:55955703-55955722 Msa0078430:intron 45.0%
CAACGGCTACATGAACAAGC+AGG - chr1_2:55954871-55954890 Msa0078430:CDS 50.0%
GAGCTCAACCCTTCACAAAG+TGG + chr1_2:55955020-55955039 None:intergenic 50.0%
CAACGGCTACATGAACAAGC+AGG - chr1_2:55954871-55954890 Msa0078430:CDS 50.0%
GAGCTCAACCCTTCACAAAG+TGG + chr1_2:55955020-55955039 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 55954797 55955815 55954797 ID=Msa0078430;Name=Msa0078430
chr1_2 mRNA 55954797 55955815 55954797 ID=Msa0078430-mRNA-1;Parent=Msa0078430;Name=Msa0078430-mRNA-1;_AED=0.32;_eAED=0.32;_QI=84|0|0.5|1|1|1|2|0|79
chr1_2 exon 55954797 55955024 55954797 ID=Msa0078430-mRNA-1:exon:17458;Parent=Msa0078430-mRNA-1
chr1_2 exon 55955720 55955815 55955720 ID=Msa0078430-mRNA-1:exon:17457;Parent=Msa0078430-mRNA-1
chr1_2 five_prime_UTR 55955732 55955815 55955732 ID=Msa0078430-mRNA-1:five_prime_utr;Parent=Msa0078430-mRNA-1
chr1_2 CDS 55955720 55955731 55955720 ID=Msa0078430-mRNA-1:cds;Parent=Msa0078430-mRNA-1
chr1_2 CDS 55954797 55955024 55954797 ID=Msa0078430-mRNA-1:cds;Parent=Msa0078430-mRNA-1
Gene Sequence

>Msa0078430

ATGAACAAGCAGGGAAGAAATGGAAGAGGTTTTCTGTTTTCACATGCAATGAATGTCACAATAGGGCCAAAAGTAGACATGGAATTCATGACAGGTCTCCATACAATTGCTGATGTTTACTGTTGTGACTGCAATCAAGTGCTTGGTTGGAAGTTTGAGAGAGTCTATGAGGAAACTCAAAAGTACAAGGAAGGCAAATACATTCTTGAGAAGTCTAAAATTGTTAGGGAAAGCTTGTAA

Protein sequence

>Msa0078430

MNKQGRNGRGFLFSHAMNVTIGPKVDMEFMTGLHTIADVYCCDCNQVLGWKFERVYEETQKYKEGKYILEKSKIVRESL*