AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0618290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618290 MtrunA17_Chr8g0380211 94.505 182 10 0 1 182 300 481 2.68e-93 279
Msa0618290 MtrunA17_Chr8g0380191 87.363 182 18 1 1 182 144 320 1.56e-92 272
Msa0618290 MtrunA17_Chr3g0139541 69.274 179 55 0 1 179 291 469 9.26e-79 242
Msa0618290 MtrunA17_Chr8g0373471 70.166 181 53 1 1 181 291 470 1.49e-73 228
Msa0618290 MtrunA17_Chr5g0403901 70.056 177 53 0 1 177 293 469 8.24e-63 201
Msa0618290 MtrunA17_Chr1g0157751 56.818 176 76 0 1 176 280 455 1.04e-54 179
Msa0618290 MtrunA17_Chr7g0265481 52.198 182 81 1 2 177 279 460 5.62e-43 149
Msa0618290 MtrunA17_Chr1g0179621 49.451 182 86 1 2 177 282 463 2.60e-39 139
Msa0618290 MtrunA17_Chr3g0139621 55.462 119 31 1 11 107 27 145 3.92e-28 102
Msa0618290 MtrunA17_Chr2g0280401 30.387 181 117 3 1 176 290 466 5.16e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618290 AT2G25737.1 71.508 179 51 0 1 179 297 475 9.51e-82 250
Msa0618290 AT2G36630.1 48.901 182 87 1 2 177 276 457 2.32e-39 139
Msa0618290 AT2G25737.2 69.333 75 23 0 1 75 297 371 1.17e-23 96.3
Msa0618290 AT1G11540.1 39.098 133 81 0 44 176 235 367 1.12e-13 68.6
Msa0618290 AT1G11540.2 39.098 133 81 0 44 176 318 450 1.86e-13 67.8
Msa0618290 AT1G61740.1 35.338 133 86 0 44 176 326 458 9.70e-12 63.2
Msa0618290 AT4G21250.1 33.540 161 103 1 20 176 289 449 3.18e-11 61.6

Find 53 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGCTTATTGCCATATT+AGG 0.163678 4_2:-69203668 Msa0618290:CDS
CTTGGTGGAGGGTTTATCTT+AGG 0.185544 4_2:-69204108 Msa0618290:CDS
TGCATTCAAACACACGTTTA+AGG 0.212293 4_2:+69202953 None:intergenic
TATATTACATGTTAGAAAAT+TGG 0.215788 4_2:+69202917 None:intergenic
ATTTGTTTGTTGATCTCCTT+TGG 0.293134 4_2:+69204203 None:intergenic
AATTGAAGCTCTCCCTAATA+TGG 0.302872 4_2:+69203655 None:intergenic
GCATATCAAATCTAATTAAA+AGG 0.306853 4_2:-69203174 Msa0618290:CDS
AAGGAGATCAACAAACAAAT+TGG 0.317337 4_2:-69204200 Msa0618290:CDS
GGCTTGCTTGGTCTTGGTGG+AGG 0.362627 4_2:-69204120 Msa0618290:CDS
GAAAGCTTATTGCCATATTA+GGG 0.364933 4_2:-69203667 Msa0618290:CDS
AACAAAGAGTACATGGGATT+TGG 0.369556 4_2:-69203145 Msa0618290:CDS
ATCTTAGGGCCACTATTCAT+TGG 0.374272 4_2:-69204093 Msa0618290:CDS
TGCACTTGTTGGGCAACATT+TGG 0.384364 4_2:-69203693 Msa0618290:CDS
ACTTATGTGCGTACGGGGTT+AGG 0.385870 4_2:-69203120 Msa0618290:CDS
GCAACTATTGCTGCACTTGT+TGG 0.388230 4_2:-69203704 Msa0618290:CDS
ATCGGTGGCTTGCTTGGTCT+TGG 0.395082 4_2:-69204126 Msa0618290:CDS
GCTTGCTTGGTCTTGGTGGA+GGG 0.410598 4_2:-69204119 Msa0618290:CDS
CCACTCACTCCTACAGCTAT+AGG 0.412332 4_2:+69204270 None:intergenic
CTTCAAATTACAGGTGGAAC+AGG 0.416473 4_2:-69203196 Msa0618290:intron
GGGCCACTATTCATTGGACT+TGG 0.419608 4_2:-69204087 Msa0618290:CDS
CAACTATTGCTGCACTTGTT+GGG 0.433867 4_2:-69203703 Msa0618290:CDS
GGAATAATCGGTGGCTTGCT+TGG 0.437494 4_2:-69204132 Msa0618290:CDS
TGTTCATGTGGCATAATTGC+TGG 0.437661 4_2:-69204153 Msa0618290:CDS
GTGGTTGGAAATCAGCATTT+AGG 0.446065 4_2:-69203000 Msa0618290:three_prime_UTR
CCTACCCTTACCATATGGAA+TGG 0.459474 4_2:+69203827 None:intergenic
GGCATAATTGCTGGAATAAT+CGG 0.460672 4_2:-69204144 Msa0618290:CDS
TTAGAAAATTGGAACTAAGT+TGG 0.463514 4_2:+69202928 None:intergenic
GGTGGCTTGCTTGGTCTTGG+TGG 0.463574 4_2:-69204123 Msa0618290:CDS
TTGGTGGAGGGTTTATCTTA+GGG 0.471899 4_2:-69204107 Msa0618290:CDS
TTTGGTAACTTATGTGCGTA+CGG 0.475564 4_2:-69203127 Msa0618290:CDS
TTGGTAACTTATGTGCGTAC+GGG 0.479683 4_2:-69203126 Msa0618290:CDS
CTACCCTTACCATATGGAAT+GGG 0.487368 4_2:+69203828 None:intergenic
GCTTGAAAACAAAGAGTACA+TGG 0.518457 4_2:-69203152 Msa0618290:CDS
TTCTGCATCTATGTCTGTGG+TGG 0.525724 4_2:-69203874 Msa0618290:CDS
TGTAGGAGTGAGTGGATATG+AGG 0.525980 4_2:-69204262 Msa0618290:CDS
ATCAATATGTGTAAAACTTG+AGG 0.531622 4_2:-69203085 Msa0618290:three_prime_UTR
TTTCAGGTACCTATAGCTGT+AGG 0.544579 4_2:-69204279 None:intergenic
AAAAGTACTACATGTGTGGT+TGG 0.547002 4_2:-69203015 Msa0618290:three_prime_UTR
TTAATTCTATATTGTTCATG+TGG 0.547087 4_2:-69204165 Msa0618290:CDS
TAGAATTAAGGTACCTGAGG+AGG 0.548268 4_2:+69204060 None:intergenic
TGGACTTGGAATCCCTCCTC+AGG 0.548917 4_2:-69204073 Msa0618290:intron
CCTATAGCTGTAGGAGTGAG+TGG 0.552521 4_2:-69204270 Msa0618290:CDS
AAATCAGCATTTAGGACATA+CGG 0.552529 4_2:-69202992 Msa0618290:three_prime_UTR
TTTGTGAGTGGTATATCACT+AGG 0.562499 4_2:-69203605 Msa0618290:intron
AGAAAATGTCATTGCAAAAG+TGG 0.570665 4_2:+69203893 None:intergenic
ATTCCAAGTCCAATGAATAG+TGG 0.572626 4_2:+69204084 None:intergenic
AAAAGAGTTATAGCTTCCAA+AGG 0.579010 4_2:-69204219 Msa0618290:CDS
GAATGGGAAAACGTTTCAGA+AGG 0.599938 4_2:+69203844 None:intergenic
AGAATTAAGGTACCTGAGGA+GGG 0.621634 4_2:+69204061 None:intergenic
TGGTAACTTATGTGCGTACG+GGG 0.625809 4_2:-69203125 Msa0618290:CDS
ATAATTGCTGGAATAATCGG+TGG 0.658335 4_2:-69204141 Msa0618290:CDS
CTTGAAAACAAAGAGTACAT+GGG 0.660526 4_2:-69203151 Msa0618290:CDS
TATCAAAAGTACTACATGTG+TGG 0.725348 4_2:-69203019 Msa0618290:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGTAAGTAATTATTTTT+TGG - chr4_2:69203890-69203909 Msa0618290:CDS 10.0%
!!! AAATGTAAGTAATTATTTTT+TGG - chr4_2:69203890-69203909 Msa0618290:CDS 10.0%
!!! AGATTTTATGAAAAAGAAAA+GGG + chr4_2:69203137-69203156 None:intergenic 15.0%
!!! TAGATTTTATGAAAAAGAAA+AGG + chr4_2:69203138-69203157 None:intergenic 15.0%
!! TATATTACATGTTAGAAAAT+TGG + chr4_2:69204253-69204272 None:intergenic 15.0%
!!! AGATTTTATGAAAAAGAAAA+GGG + chr4_2:69203137-69203156 None:intergenic 15.0%
!!! TAGATTTTATGAAAAAGAAA+AGG + chr4_2:69203138-69203157 None:intergenic 15.0%
!! TATATTACATGTTAGAAAAT+TGG + chr4_2:69204253-69204272 None:intergenic 15.0%
!! TTAATTCTATATTGTTCATG+TGG - chr4_2:69203002-69203021 Msa0618290:three_prime_UTR 20.0%
!!! GATTGAAGAATTTTCTATTA+AGG - chr4_2:69203236-69203255 Msa0618290:intron 20.0%
!!! TATGTCTTGTTTTGTTAAAA+TGG - chr4_2:69203630-69203649 Msa0618290:CDS 20.0%
!!! CTAAAATGAATTTGTTTAAG+TGG - chr4_2:69203707-69203726 Msa0618290:CDS 20.0%
!! GCATATCAAATCTAATTAAA+AGG - chr4_2:69203993-69204012 Msa0618290:intron 20.0%
!! TTAATTCTATATTGTTCATG+TGG - chr4_2:69203002-69203021 Msa0618290:three_prime_UTR 20.0%
!!! GATTGAAGAATTTTCTATTA+AGG - chr4_2:69203236-69203255 Msa0618290:intron 20.0%
!!! TATGTCTTGTTTTGTTAAAA+TGG - chr4_2:69203630-69203649 Msa0618290:CDS 20.0%
!!! CTAAAATGAATTTGTTTAAG+TGG - chr4_2:69203707-69203726 Msa0618290:CDS 20.0%
!! GCATATCAAATCTAATTAAA+AGG - chr4_2:69203993-69204012 Msa0618290:intron 20.0%
!! TGAAGAATTTTCTATTAAGG+TGG - chr4_2:69203239-69203258 Msa0618290:intron 25.0%
! TTGTTAAAATGGAGATACAA+AGG - chr4_2:69203641-69203660 Msa0618290:CDS 25.0%
!! ATAGTGAGAGAAAAAAGTTT+TGG - chr4_2:69203782-69203801 Msa0618290:intron 25.0%
!! ACATTTGAAGTAAACAAACA+AGG + chr4_2:69203876-69203895 None:intergenic 25.0%
!!! TTTTTTTCCCTTCAAATTAC+AGG - chr4_2:69203962-69203981 Msa0618290:intron 25.0%
! ATCAATATGTGTAAAACTTG+AGG - chr4_2:69204082-69204101 Msa0618290:CDS 25.0%
! TTAGAAAATTGGAACTAAGT+TGG + chr4_2:69204242-69204261 None:intergenic 25.0%
!! TGAAGAATTTTCTATTAAGG+TGG - chr4_2:69203239-69203258 Msa0618290:intron 25.0%
! TTGTTAAAATGGAGATACAA+AGG - chr4_2:69203641-69203660 Msa0618290:CDS 25.0%
!! ATAGTGAGAGAAAAAAGTTT+TGG - chr4_2:69203782-69203801 Msa0618290:intron 25.0%
!! ACATTTGAAGTAAACAAACA+AGG + chr4_2:69203876-69203895 None:intergenic 25.0%
!!! TTTTTTTCCCTTCAAATTAC+AGG - chr4_2:69203962-69203981 Msa0618290:intron 25.0%
! ATCAATATGTGTAAAACTTG+AGG - chr4_2:69204082-69204101 Msa0618290:CDS 25.0%
! TTAGAAAATTGGAACTAAGT+TGG + chr4_2:69204242-69204261 None:intergenic 25.0%
! GAGGCTATACTTTTATACAA+AGG - chr4_2:69202924-69202943 Msa0618290:three_prime_UTR 30.0%
AAAAGAGTTATAGCTTCCAA+AGG - chr4_2:69202948-69202967 Msa0618290:three_prime_UTR 30.0%
! ATTTGTTTGTTGATCTCCTT+TGG + chr4_2:69202967-69202986 None:intergenic 30.0%
AAGGAGATCAACAAACAAAT+TGG - chr4_2:69202967-69202986 Msa0618290:three_prime_UTR 30.0%
AGAAAATGTCATTGCAAAAG+TGG + chr4_2:69203277-69203296 None:intergenic 30.0%
CACTAAAAGAAGGCAATAAT+TGG - chr4_2:69203378-69203397 Msa0618290:intron 30.0%
AGAAAGCTTATTGCCATATT+AGG - chr4_2:69203499-69203518 Msa0618290:intron 30.0%
GAAAGCTTATTGCCATATTA+GGG - chr4_2:69203500-69203519 Msa0618290:intron 30.0%
TGCTAGTCATAATTACAAAG+AGG + chr4_2:69203607-69203626 None:intergenic 30.0%
! CTTGAAAACAAAGAGTACAT+GGG - chr4_2:69204016-69204035 Msa0618290:intron 30.0%
!!! ATAGTTTTGTGTTGTCAAGT+TGG - chr4_2:69204125-69204144 Msa0618290:CDS 30.0%
!! TATCAAAAGTACTACATGTG+TGG - chr4_2:69204148-69204167 Msa0618290:CDS 30.0%
! AAATCAGCATTTAGGACATA+CGG - chr4_2:69204175-69204194 Msa0618290:CDS 30.0%
! GAGGCTATACTTTTATACAA+AGG - chr4_2:69202924-69202943 Msa0618290:three_prime_UTR 30.0%
AAAAGAGTTATAGCTTCCAA+AGG - chr4_2:69202948-69202967 Msa0618290:three_prime_UTR 30.0%
! ATTTGTTTGTTGATCTCCTT+TGG + chr4_2:69202967-69202986 None:intergenic 30.0%
AAGGAGATCAACAAACAAAT+TGG - chr4_2:69202967-69202986 Msa0618290:three_prime_UTR 30.0%
AGAAAATGTCATTGCAAAAG+TGG + chr4_2:69203277-69203296 None:intergenic 30.0%
CACTAAAAGAAGGCAATAAT+TGG - chr4_2:69203378-69203397 Msa0618290:intron 30.0%
AGAAAGCTTATTGCCATATT+AGG - chr4_2:69203499-69203518 Msa0618290:intron 30.0%
GAAAGCTTATTGCCATATTA+GGG - chr4_2:69203500-69203519 Msa0618290:intron 30.0%
TGCTAGTCATAATTACAAAG+AGG + chr4_2:69203607-69203626 None:intergenic 30.0%
! CTTGAAAACAAAGAGTACAT+GGG - chr4_2:69204016-69204035 Msa0618290:intron 30.0%
!!! ATAGTTTTGTGTTGTCAAGT+TGG - chr4_2:69204125-69204144 Msa0618290:CDS 30.0%
!! TATCAAAAGTACTACATGTG+TGG - chr4_2:69204148-69204167 Msa0618290:CDS 30.0%
! AAATCAGCATTTAGGACATA+CGG - chr4_2:69204175-69204194 Msa0618290:CDS 30.0%
GGCATAATTGCTGGAATAAT+CGG - chr4_2:69203023-69203042 Msa0618290:three_prime_UTR 35.0%
ATAATTGCTGGAATAATCGG+TGG - chr4_2:69203026-69203045 Msa0618290:three_prime_UTR 35.0%
ATTCCAAGTCCAATGAATAG+TGG + chr4_2:69203086-69203105 None:intergenic 35.0%
! GAAAAGGGTACGTAGAATTA+AGG + chr4_2:69203122-69203141 None:intergenic 35.0%
! ATTTTCTGCATCTATGTCTG+TGG - chr4_2:69203290-69203309 Msa0618290:intron 35.0%
! AAACGTTTTCCCATTCCATA+TGG - chr4_2:69203330-69203349 Msa0618290:intron 35.0%
! TTTTCCCATTCCATATGGTA+AGG - chr4_2:69203335-69203354 Msa0618290:intron 35.0%
TTTCCCATTCCATATGGTAA+GGG - chr4_2:69203336-69203355 Msa0618290:intron 35.0%
! TTTTTCCTACCCTTACCATA+TGG + chr4_2:69203348-69203367 None:intergenic 35.0%
ATATGGTAAGGGTAGGAAAA+AGG - chr4_2:69203347-69203366 Msa0618290:intron 35.0%
TATGGTAAGGGTAGGAAAAA+GGG - chr4_2:69203348-69203367 Msa0618290:intron 35.0%
GGGATTGTTACACTAAAAGA+AGG - chr4_2:69203368-69203387 Msa0618290:intron 35.0%
AATTGAAGCTCTCCCTAATA+TGG + chr4_2:69203515-69203534 None:intergenic 35.0%
TTTGTGAGTGGTATATCACT+AGG - chr4_2:69203562-69203581 Msa0618290:intron 35.0%
! TTTTCCCTTCAAATTACAGG+TGG - chr4_2:69203965-69203984 Msa0618290:intron 35.0%
TGTTCCACCTGTAATTTGAA+GGG + chr4_2:69203972-69203991 None:intergenic 35.0%
! GCTTGAAAACAAAGAGTACA+TGG - chr4_2:69204015-69204034 Msa0618290:intron 35.0%
! AACAAAGAGTACATGGGATT+TGG - chr4_2:69204022-69204041 Msa0618290:intron 35.0%
! TTTGGTAACTTATGTGCGTA+CGG - chr4_2:69204040-69204059 Msa0618290:intron 35.0%
!! AAAAGTACTACATGTGTGGT+TGG - chr4_2:69204152-69204171 Msa0618290:CDS 35.0%
TGCATTCAAACACACGTTTA+AGG + chr4_2:69204217-69204236 None:intergenic 35.0%
GGCATAATTGCTGGAATAAT+CGG - chr4_2:69203023-69203042 Msa0618290:three_prime_UTR 35.0%
ATAATTGCTGGAATAATCGG+TGG - chr4_2:69203026-69203045 Msa0618290:three_prime_UTR 35.0%
ATTCCAAGTCCAATGAATAG+TGG + chr4_2:69203086-69203105 None:intergenic 35.0%
! GAAAAGGGTACGTAGAATTA+AGG + chr4_2:69203122-69203141 None:intergenic 35.0%
! ATTTTCTGCATCTATGTCTG+TGG - chr4_2:69203290-69203309 Msa0618290:intron 35.0%
! AAACGTTTTCCCATTCCATA+TGG - chr4_2:69203330-69203349 Msa0618290:intron 35.0%
! TTTTCCCATTCCATATGGTA+AGG - chr4_2:69203335-69203354 Msa0618290:intron 35.0%
TTTCCCATTCCATATGGTAA+GGG - chr4_2:69203336-69203355 Msa0618290:intron 35.0%
! TTTTTCCTACCCTTACCATA+TGG + chr4_2:69203348-69203367 None:intergenic 35.0%
ATATGGTAAGGGTAGGAAAA+AGG - chr4_2:69203347-69203366 Msa0618290:intron 35.0%
TATGGTAAGGGTAGGAAAAA+GGG - chr4_2:69203348-69203367 Msa0618290:intron 35.0%
GGGATTGTTACACTAAAAGA+AGG - chr4_2:69203368-69203387 Msa0618290:intron 35.0%
AATTGAAGCTCTCCCTAATA+TGG + chr4_2:69203515-69203534 None:intergenic 35.0%
TTTGTGAGTGGTATATCACT+AGG - chr4_2:69203562-69203581 Msa0618290:intron 35.0%
! TTTTCCCTTCAAATTACAGG+TGG - chr4_2:69203965-69203984 Msa0618290:intron 35.0%
TGTTCCACCTGTAATTTGAA+GGG + chr4_2:69203972-69203991 None:intergenic 35.0%
! GCTTGAAAACAAAGAGTACA+TGG - chr4_2:69204015-69204034 Msa0618290:intron 35.0%
! AACAAAGAGTACATGGGATT+TGG - chr4_2:69204022-69204041 Msa0618290:intron 35.0%
! TTTGGTAACTTATGTGCGTA+CGG - chr4_2:69204040-69204059 Msa0618290:intron 35.0%
!! AAAAGTACTACATGTGTGGT+TGG - chr4_2:69204152-69204171 Msa0618290:CDS 35.0%
TGCATTCAAACACACGTTTA+AGG + chr4_2:69204217-69204236 None:intergenic 35.0%
TGTTCATGTGGCATAATTGC+TGG - chr4_2:69203014-69203033 Msa0618290:three_prime_UTR 40.0%
TTGGTGGAGGGTTTATCTTA+GGG - chr4_2:69203060-69203079 Msa0618290:three_prime_UTR 40.0%
ATCTTAGGGCCACTATTCAT+TGG - chr4_2:69203074-69203093 Msa0618290:three_prime_UTR 40.0%
! AGAATTAAGGTACCTGAGGA+GGG + chr4_2:69203109-69203128 None:intergenic 40.0%
! TAGAATTAAGGTACCTGAGG+AGG + chr4_2:69203110-69203129 None:intergenic 40.0%
! ACGTAGAATTAAGGTACCTG+AGG + chr4_2:69203113-69203132 None:intergenic 40.0%
GAATGGGAAAACGTTTCAGA+AGG + chr4_2:69203326-69203345 None:intergenic 40.0%
CTACCCTTACCATATGGAAT+GGG + chr4_2:69203342-69203361 None:intergenic 40.0%
CAACTATTGCTGCACTTGTT+GGG - chr4_2:69203464-69203483 Msa0618290:intron 40.0%
ACCACTCACAAAAACTGTGA+GGG + chr4_2:69203554-69203573 None:intergenic 40.0%
TACCACTCACAAAAACTGTG+AGG + chr4_2:69203555-69203574 None:intergenic 40.0%
! GGTGCTCATCTTAATTTGAC+TGG - chr4_2:69203803-69203822 Msa0618290:intron 40.0%
CTGTTCCACCTGTAATTTGA+AGG + chr4_2:69203973-69203992 None:intergenic 40.0%
CTTCAAATTACAGGTGGAAC+AGG - chr4_2:69203971-69203990 Msa0618290:intron 40.0%
! TTGGTAACTTATGTGCGTAC+GGG - chr4_2:69204041-69204060 Msa0618290:intron 40.0%
GTGGTTGGAAATCAGCATTT+AGG - chr4_2:69204167-69204186 Msa0618290:CDS 40.0%
TGTTCATGTGGCATAATTGC+TGG - chr4_2:69203014-69203033 Msa0618290:three_prime_UTR 40.0%
TTGGTGGAGGGTTTATCTTA+GGG - chr4_2:69203060-69203079 Msa0618290:three_prime_UTR 40.0%
ATCTTAGGGCCACTATTCAT+TGG - chr4_2:69203074-69203093 Msa0618290:three_prime_UTR 40.0%
! AGAATTAAGGTACCTGAGGA+GGG + chr4_2:69203109-69203128 None:intergenic 40.0%
! TAGAATTAAGGTACCTGAGG+AGG + chr4_2:69203110-69203129 None:intergenic 40.0%
! ACGTAGAATTAAGGTACCTG+AGG + chr4_2:69203113-69203132 None:intergenic 40.0%
GAATGGGAAAACGTTTCAGA+AGG + chr4_2:69203326-69203345 None:intergenic 40.0%
CTACCCTTACCATATGGAAT+GGG + chr4_2:69203342-69203361 None:intergenic 40.0%
CAACTATTGCTGCACTTGTT+GGG - chr4_2:69203464-69203483 Msa0618290:intron 40.0%
ACCACTCACAAAAACTGTGA+GGG + chr4_2:69203554-69203573 None:intergenic 40.0%
TACCACTCACAAAAACTGTG+AGG + chr4_2:69203555-69203574 None:intergenic 40.0%
! GGTGCTCATCTTAATTTGAC+TGG - chr4_2:69203803-69203822 Msa0618290:intron 40.0%
CTGTTCCACCTGTAATTTGA+AGG + chr4_2:69203973-69203992 None:intergenic 40.0%
CTTCAAATTACAGGTGGAAC+AGG - chr4_2:69203971-69203990 Msa0618290:intron 40.0%
! TTGGTAACTTATGTGCGTAC+GGG - chr4_2:69204041-69204060 Msa0618290:intron 40.0%
GTGGTTGGAAATCAGCATTT+AGG - chr4_2:69204167-69204186 Msa0618290:CDS 40.0%
TGTAGGAGTGAGTGGATATG+AGG - chr4_2:69202905-69202924 Msa0618290:three_prime_UTR 45.0%
CTTGGTGGAGGGTTTATCTT+AGG - chr4_2:69203059-69203078 Msa0618290:three_prime_UTR 45.0%
TTCTGCATCTATGTCTGTGG+TGG - chr4_2:69203293-69203312 Msa0618290:intron 45.0%
CCTACCCTTACCATATGGAA+TGG + chr4_2:69203343-69203362 None:intergenic 45.0%
CCATTCCATATGGTAAGGGT+AGG - chr4_2:69203340-69203359 Msa0618290:intron 45.0%
GCAACTATTGCTGCACTTGT+TGG - chr4_2:69203463-69203482 Msa0618290:intron 45.0%
! TGCACTTGTTGGGCAACATT+TGG - chr4_2:69203474-69203493 Msa0618290:intron 45.0%
TGGTAACTTATGTGCGTACG+GGG - chr4_2:69204042-69204061 Msa0618290:intron 45.0%
TGTAGGAGTGAGTGGATATG+AGG - chr4_2:69202905-69202924 Msa0618290:three_prime_UTR 45.0%
CTTGGTGGAGGGTTTATCTT+AGG - chr4_2:69203059-69203078 Msa0618290:three_prime_UTR 45.0%
TTCTGCATCTATGTCTGTGG+TGG - chr4_2:69203293-69203312 Msa0618290:intron 45.0%
CCTACCCTTACCATATGGAA+TGG + chr4_2:69203343-69203362 None:intergenic 45.0%
CCATTCCATATGGTAAGGGT+AGG - chr4_2:69203340-69203359 Msa0618290:intron 45.0%
GCAACTATTGCTGCACTTGT+TGG - chr4_2:69203463-69203482 Msa0618290:intron 45.0%
! TGCACTTGTTGGGCAACATT+TGG - chr4_2:69203474-69203493 Msa0618290:intron 45.0%
TGGTAACTTATGTGCGTACG+GGG - chr4_2:69204042-69204061 Msa0618290:intron 45.0%
CCACTCACTCCTACAGCTAT+AGG + chr4_2:69202900-69202919 None:intergenic 50.0%
CCTATAGCTGTAGGAGTGAG+TGG - chr4_2:69202897-69202916 Msa0618290:three_prime_UTR 50.0%
!! GGAATAATCGGTGGCTTGCT+TGG - chr4_2:69203035-69203054 Msa0618290:three_prime_UTR 50.0%
GGGCCACTATTCATTGGACT+TGG - chr4_2:69203080-69203099 Msa0618290:three_prime_UTR 50.0%
! GCCCTCACAGTTTTTGTGAG+TGG - chr4_2:69203550-69203569 Msa0618290:intron 50.0%
ACTTATGTGCGTACGGGGTT+AGG - chr4_2:69204047-69204066 Msa0618290:intron 50.0%
CCACTCACTCCTACAGCTAT+AGG + chr4_2:69202900-69202919 None:intergenic 50.0%
CCTATAGCTGTAGGAGTGAG+TGG - chr4_2:69202897-69202916 Msa0618290:three_prime_UTR 50.0%
!! GGAATAATCGGTGGCTTGCT+TGG - chr4_2:69203035-69203054 Msa0618290:three_prime_UTR 50.0%
GGGCCACTATTCATTGGACT+TGG - chr4_2:69203080-69203099 Msa0618290:three_prime_UTR 50.0%
! GCCCTCACAGTTTTTGTGAG+TGG - chr4_2:69203550-69203569 Msa0618290:intron 50.0%
ACTTATGTGCGTACGGGGTT+AGG - chr4_2:69204047-69204066 Msa0618290:intron 50.0%
!! ATCGGTGGCTTGCTTGGTCT+TGG - chr4_2:69203041-69203060 Msa0618290:three_prime_UTR 55.0%
!! GCTTGCTTGGTCTTGGTGGA+GGG - chr4_2:69203048-69203067 Msa0618290:three_prime_UTR 55.0%
TGGACTTGGAATCCCTCCTC+AGG - chr4_2:69203094-69203113 Msa0618290:three_prime_UTR 55.0%
!! ATCGGTGGCTTGCTTGGTCT+TGG - chr4_2:69203041-69203060 Msa0618290:three_prime_UTR 55.0%
!! GCTTGCTTGGTCTTGGTGGA+GGG - chr4_2:69203048-69203067 Msa0618290:three_prime_UTR 55.0%
TGGACTTGGAATCCCTCCTC+AGG - chr4_2:69203094-69203113 Msa0618290:three_prime_UTR 55.0%
!! GGTGGCTTGCTTGGTCTTGG+TGG - chr4_2:69203044-69203063 Msa0618290:three_prime_UTR 60.0%
!! GGCTTGCTTGGTCTTGGTGG+AGG - chr4_2:69203047-69203066 Msa0618290:three_prime_UTR 60.0%
!! GGTGGCTTGCTTGGTCTTGG+TGG - chr4_2:69203044-69203063 Msa0618290:three_prime_UTR 60.0%
!! GGCTTGCTTGGTCTTGGTGG+AGG - chr4_2:69203047-69203066 Msa0618290:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 69202894 69204295 69202894 ID=Msa0618290;Name=Msa0618290
chr4_2 mRNA 69202894 69204295 69202894 ID=Msa0618290-mRNA-1;Parent=Msa0618290;Name=Msa0618290-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|1|0.75|1|1|1|4|220|182
chr4_2 exon 69202894 69203205 69202894 ID=Msa0618290-mRNA-1:exon:20031;Parent=Msa0618290-mRNA-1
chr4_2 exon 69203606 69203746 69203606 ID=Msa0618290-mRNA-1:exon:20030;Parent=Msa0618290-mRNA-1
chr4_2 exon 69203838 69203931 69203838 ID=Msa0618290-mRNA-1:exon:20029;Parent=Msa0618290-mRNA-1
chr4_2 exon 69204074 69204295 69204074 ID=Msa0618290-mRNA-1:exon:20028;Parent=Msa0618290-mRNA-1
chr4_2 CDS 69204074 69204295 69204074 ID=Msa0618290-mRNA-1:cds;Parent=Msa0618290-mRNA-1
chr4_2 CDS 69203838 69203931 69203838 ID=Msa0618290-mRNA-1:cds;Parent=Msa0618290-mRNA-1
chr4_2 CDS 69203606 69203746 69203606 ID=Msa0618290-mRNA-1:cds;Parent=Msa0618290-mRNA-1
chr4_2 CDS 69203114 69203205 69203114 ID=Msa0618290-mRNA-1:cds;Parent=Msa0618290-mRNA-1
chr4_2 three_prime_UTR 69202894 69203113 69202894 ID=Msa0618290-mRNA-1:three_prime_utr;Parent=Msa0618290-mRNA-1
Gene Sequence

>Msa0618290

GTACCTATAGCTGTAGGAGTGAGTGGATATGAGGCTATACTTTTATACAAAGGAAAAAGAGTTATAGCTTCCAAAGGAGATCAACAAACAAATTGGAGTGTGAAGCAGTTAATTCTATATTGTTCATGTGGCATAATTGCTGGAATAATCGGTGGCTTGCTTGGTCTTGGTGGAGGGTTTATCTTAGGGCCACTATTCATTGGACTTGGAATCCCTCCTCAGGTGGCAAGTGCTACATCCACTTTTGCAATGACATTTTCTGCATCTATGTCTGTGGTGGAATATTACCTTCTGAAACGTTTTCCCATTCCATATGCTCTTTACTTTGTAGCTGTAGCAACTATTGCTGCACTTGTTGGGCAACATTTGGTGAGAAAGCTTATTGCCATATTAGGGAGAGCTTCAATTATCATTTTCATCTTAGCCCTCACAGTTTTTGTGAGTGGTATATCACTAGGTGGAACAGGCATATCAAATCTAATTAAAAGGCTTGAAAACAAAGAGTACATGGGATTTGGTAACTTATGTGCGTACGGGGTTAGGAATTAA
Protein sequence

>Msa0618290

VPIAVGVSGYEAILLYKGKRVIASKGDQQTNWSVKQLILYCSCGIIAGIIGGLLGLGGGFILGPLFIGLGIPPQVASATSTFAMTFSASMSVVEYYLLKRFPIPYALYFVAVATIAALVGQHLVRKLIAILGRASIIIFILALTVFVSGISLGGTGISNLIKRLENKEYMGFGNLCAYGVRN*