AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0127660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127660 MtrunA17_Chr1g0191261 97.026 269 8 0 4 272 108 376 1.67e-179 502
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 132 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCCTAACCTTATTAAAA+TGG 0.144741 1_3:+57144663 Msa0127660:three_prime_UTR
CCAGAACTAGTTCCTGTTAA+TGG 0.166308 1_3:+57142818 Msa0127660:CDS
CTGCCACCTTGTCAAGATTT+TGG 0.169575 1_3:+57144013 Msa0127660:three_prime_UTR
GGCACTTATCTGTGTCTTCT+TGG 0.212884 1_3:+57145213 Msa0127660:three_prime_UTR
CGATACTTTGTATATAATAT+TGG 0.236274 1_3:+57144618 Msa0127660:three_prime_UTR
CCGTTTACACTCTTGATATC+TGG 0.242302 1_3:+57145240 Msa0127660:three_prime_UTR
ATACAAACGCAACGTGATTA+TGG 0.266988 1_3:-57145414 None:intergenic
GCAAGATAGTGAAATATAAT+TGG 0.268359 1_3:-57144232 None:intergenic
TTCAACCCTGCTTCTTCTCT+TGG 0.273735 1_3:-57143430 None:intergenic
GCTTGCTGTAAATAACTATT+AGG 0.282182 1_3:+57144117 Msa0127660:three_prime_UTR
GGACTTAAATTTGTCATGTT+AGG 0.286389 1_3:+57142303 None:intergenic
TGCCTCAAGTTTGTCATATT+AGG 0.288656 1_3:+57145300 Msa0127660:three_prime_UTR
TTCTTTGAGTCTCCATTAAC+AGG 0.295800 1_3:-57142830 None:intergenic
GTTGAAGGTAGAATATGATA+TGG 0.309168 1_3:+57143378 Msa0127660:CDS
TGCAGAGATTGAAGAGGATA+TGG 0.310428 1_3:+57143354 Msa0127660:CDS
TATGGGGTTGGATGCTATTA+AGG 0.319281 1_3:+57143396 Msa0127660:CDS
GGAGCCAGCAAAGAATCTTT+TGG 0.334724 1_3:+57144502 Msa0127660:three_prime_UTR
TCAAAATGTTAGTATCTTCC+TGG 0.337913 1_3:-57145275 None:intergenic
TTTCGTCTAACCATGGGTTC+AGG 0.347161 1_3:-57142911 None:intergenic
CCTCCTCTCCTCTGTTAACT+TGG 0.348926 1_3:-57144791 None:intergenic
GACTTAAATTTGTCATGTTA+GGG 0.349097 1_3:+57142304 None:intergenic
TTTCCTAACCTTATTAAAAT+GGG 0.349263 1_3:+57144664 Msa0127660:three_prime_UTR
CCATTAACAGGAACTAGTTC+TGG 0.359456 1_3:-57142818 None:intergenic
AGGGGAAAGTGGTGTGTATA+AGG 0.371875 1_3:-57144385 None:intergenic
GCACTTATCTGTGTCTTCTT+GGG 0.372916 1_3:+57145214 Msa0127660:three_prime_UTR
TTGAGATTTGAGATGTTAGT+TGG 0.374216 1_3:+57144084 Msa0127660:three_prime_UTR
TGAAGAGGATATGGAGTTGA+AGG 0.374389 1_3:+57143363 Msa0127660:CDS
CACATAGGATTGAGCCTGTA+TGG 0.375175 1_3:-57144584 None:intergenic
GATGAAGGTGTCAAAAGATC+TGG 0.376784 1_3:+57143597 Msa0127660:CDS
CAAATGCCATAGTTCCAAAA+AGG 0.385651 1_3:-57145731 None:intergenic
TGATGAGATTAGTGGAAAAC+TGG 0.389046 1_3:+57145501 Msa0127660:three_prime_UTR
ATTGAGCCTGTATGGATGTT+AGG 0.390986 1_3:-57144576 None:intergenic
GGAACTAGTTCTGGTGAATC+AGG 0.392780 1_3:-57142809 None:intergenic
TAACCATGGGTTCAGGGGAC+TGG 0.399998 1_3:-57142904 None:intergenic
TTGAAGGTAGAATATGATAT+GGG 0.407459 1_3:+57143379 Msa0127660:CDS
ACATCCAAAAGATTCTTTGC+TGG 0.411935 1_3:-57144506 None:intergenic
CGTTTACACTCTTGATATCT+GGG 0.413556 1_3:+57145241 Msa0127660:three_prime_UTR
ATATGAACCTTATTATGCTC+TGG 0.416859 1_3:-57143307 None:intergenic
GAGATGAAGTTAAAACTAGA+TGG 0.426084 1_3:+57143871 Msa0127660:CDS
CCAGATATCAAGAGTGTAAA+CGG 0.442402 1_3:-57145240 None:intergenic
CAGGCAGATCTCATTAATAC+AGG 0.447142 1_3:+57145142 Msa0127660:intron
AAGTTCATTCATAGTTCGTC+AGG 0.448784 1_3:-57145702 None:intergenic
GGTACAAGTCTCAAAAGATC+TGG 0.453038 1_3:+57143522 Msa0127660:CDS
TAATCGAAGTTTGTCAAGGA+TGG 0.457053 1_3:+57142737 Msa0127660:intron
AGCGGAGTCAAGAGTTTCAA+AGG 0.463926 1_3:+57143813 Msa0127660:CDS
TGATGAAGCAATTGAGCATA+TGG 0.464813 1_3:+57144641 Msa0127660:three_prime_UTR
AAGAGTTTCAAAGGGTATTG+AGG 0.466356 1_3:+57143822 Msa0127660:CDS
TTATTTACAGCAAGCGATGA+AGG 0.468627 1_3:-57144109 None:intergenic
GCAGAATTTCTCACTTGTAA+AGG 0.477676 1_3:+57145439 Msa0127660:three_prime_UTR
TTCGTCTAACCATGGGTTCA+GGG 0.488202 1_3:-57142910 None:intergenic
TATATTTCACTATCTTGCTA+TGG 0.488375 1_3:+57144237 Msa0127660:three_prime_UTR
GTTAATGGAGACTCAAAGAA+TGG 0.489508 1_3:+57142833 Msa0127660:CDS
ATTGAAAACATATGACCAAA+AGG 0.489902 1_3:-57144060 None:intergenic
AATGCTCCATCTGATCTGTC+TGG 0.490201 1_3:+57145390 Msa0127660:three_prime_UTR
TACTGTCTTAATCACTGTAT+CGG 0.494218 1_3:-57143758 None:intergenic
GGAACTGAAACCAGGAAAAG+AGG 0.496214 1_3:+57143570 Msa0127660:CDS
TGAGGAGATGAACTCAGTAA+AGG 0.496472 1_3:+57143465 Msa0127660:CDS
GAGGAGATGAACTCAGTAAA+GGG 0.496692 1_3:+57143466 Msa0127660:CDS
AGGAAAAGAGGCAGAGATGA+AGG 0.499789 1_3:+57143582 Msa0127660:CDS
GTCCAAGAAAAGTTCAAGTC+GGG 0.504636 1_3:+57145164 Msa0127660:three_prime_UTR
CAATTGAGTTCACAGGGAAA+AGG 0.509750 1_3:-57145556 None:intergenic
TACTAATCAATTGAGTTCAC+AGG 0.522756 1_3:-57145563 None:intergenic
AAAGCAGAGGAACTGAAACC+TGG 0.523769 1_3:+57143637 Msa0127660:CDS
GGAGAGCCAAGAGAAGAAGC+AGG 0.526638 1_3:+57143424 Msa0127660:CDS
TGCCTTTCCATTGTTTACTG+TGG 0.527555 1_3:-57143698 None:intergenic
ACAGTAACTTGCGACTTGCT+AGG 0.530256 1_3:-57143981 None:intergenic
CGAACTATGAATGAACTTGA+GGG 0.530541 1_3:+57145707 Msa0127660:three_prime_UTR
TAGAGATTAGCATGAAAGTC+GGG 0.531474 1_3:-57144708 None:intergenic
TTGGATGCTATTAAGGATGT+AGG 0.531555 1_3:+57143403 Msa0127660:CDS
AAATGAAAACAAGGGGAAAG+TGG 0.541657 1_3:-57144396 None:intergenic
TCACGAACCTTAGTATGCTC+TGG 0.541662 1_3:-57143331 None:intergenic
CCCACTTCTATATGTCTGCA+TGG 0.542803 1_3:+57145189 Msa0127660:three_prime_UTR
CTAACCTTATTAAAATGGGG+TGG 0.544581 1_3:+57144668 Msa0127660:three_prime_UTR
ATGAAGGTGTCAAAAGATCT+GGG 0.554191 1_3:+57143598 Msa0127660:CDS
TCATATACCAGAGCATACTA+AGG 0.554759 1_3:+57143324 Msa0127660:CDS
TTCCTAACCTTATTAAAATG+GGG 0.556230 1_3:+57144665 Msa0127660:three_prime_UTR
TATTTACAGCAAGCGATGAA+GGG 0.558757 1_3:-57144108 None:intergenic
TATTGAGGAGCTGAAACTGC+TGG 0.560970 1_3:+57143837 Msa0127660:CDS
AGTACAAGGTATATAGCGGT+CGG 0.562262 1_3:+57145469 Msa0127660:three_prime_UTR
GTACAAGGTATATAGCGGTC+GGG 0.562608 1_3:+57145470 Msa0127660:three_prime_UTR
TTTATAATCGAAGTTTGTCA+AGG 0.566542 1_3:+57142733 Msa0127660:intron
AAAGCTCCTAACATCCATAC+AGG 0.567411 1_3:+57144570 Msa0127660:three_prime_UTR
GGTAGAATATGATATGGGGT+TGG 0.570375 1_3:+57143384 Msa0127660:CDS
ACGAACTATGAATGAACTTG+AGG 0.570667 1_3:+57145706 Msa0127660:three_prime_UTR
GCGGAGTCAAGAGTTTCAAA+GGG 0.572722 1_3:+57143814 Msa0127660:CDS
TAGATGACTGATGAGATTAG+TGG 0.575372 1_3:+57145493 Msa0127660:three_prime_UTR
TATGGACCAGACAGATCAGA+TGG 0.577642 1_3:-57145396 None:intergenic
GTTGAAAGTGTCAAGAGTTG+AGG 0.580023 1_3:+57143447 Msa0127660:CDS
AATTTAATCAAATGAAAACA+AGG 0.581727 1_3:-57144405 None:intergenic
AATCCAGTCCCCTGAACCCA+TGG 0.582271 1_3:+57142901 Msa0127660:CDS
ATTTAATCAAATGAAAACAA+GGG 0.588911 1_3:-57144404 None:intergenic
GTAGAGATTAGCATGAAAGT+CGG 0.592962 1_3:-57144709 None:intergenic
CCAAGTTAACAGAGGAGAGG+AGG 0.593009 1_3:+57144791 Msa0127660:three_prime_UTR
ATATAAGTTTGAATTAGAGA+AGG 0.594130 1_3:+57144472 Msa0127660:three_prime_UTR
ATTCTTTGCTGGCTCCTCTG+TGG 0.598825 1_3:-57144495 None:intergenic
AATGCAGAGGAACTGAAACC+AGG 0.603182 1_3:+57143562 Msa0127660:CDS
TTAATTATACTTTCAATGCG+TGG 0.605126 1_3:+57144307 Msa0127660:three_prime_UTR
ACTAGTTCTGGTGAATCAGG+TGG 0.606820 1_3:-57142806 None:intergenic
ACTTAAATTTGTCATGTTAG+GGG 0.607965 1_3:+57142305 None:intergenic
TATTGAAGAAAATAAGCTGA+AGG 0.613728 1_3:+57142769 Msa0127660:CDS
GGTCCAAGAAAAGTTCAAGT+CGG 0.614155 1_3:+57145163 Msa0127660:three_prime_UTR
GAGGAGGTTAAGCAATCAAG+AGG 0.616339 1_3:+57144807 Msa0127660:three_prime_UTR
TCGTCTAACCATGGGTTCAG+GGG 0.618454 1_3:-57142909 None:intergenic
ACTTGAGTCAAAGCTAAATG+AGG 0.624176 1_3:+57143894 Msa0127660:CDS
ATCCTAATATGACAAACTTG+AGG 0.624607 1_3:-57145302 None:intergenic
GACCACAGTAAACAATGGAA+AGG 0.626884 1_3:+57143696 Msa0127660:CDS
TTTAATCAAATGAAAACAAG+GGG 0.626903 1_3:-57144403 None:intergenic
TCGTGATGCAGAGATTGAAG+AGG 0.628341 1_3:+57143348 Msa0127660:CDS
ACCATGCAGACATATAGAAG+TGG 0.631855 1_3:-57145190 None:intergenic
GTTTACACTCTTGATATCTG+GGG 0.632135 1_3:+57145242 Msa0127660:three_prime_UTR
GATGAACTCAGTAAAGGGTG+CGG 0.632166 1_3:+57143471 Msa0127660:CDS
GAGAGCCAAGAGAAGAAGCA+GGG 0.633793 1_3:+57143425 Msa0127660:CDS
TAAGACAGTAAAGAATGTTG+AGG 0.634008 1_3:+57143771 Msa0127660:CDS
TGAAGGTAGAATATGATATG+GGG 0.634862 1_3:+57143380 Msa0127660:CDS
AACAATATCCAAGTTAACAG+AGG 0.640525 1_3:+57144783 Msa0127660:three_prime_UTR
ACTAATCAATTGAGTTCACA+GGG 0.640770 1_3:-57145562 None:intergenic
TGAAGAAAATAAGCTGAAGG+TGG 0.641027 1_3:+57142772 Msa0127660:CDS
TTTACACTCTTGATATCTGG+GGG 0.641415 1_3:+57145243 Msa0127660:three_prime_UTR
CCATGCAGACATATAGAAGT+GGG 0.643537 1_3:-57145189 None:intergenic
GAACTCAGTAAAGGGTGCGG+AGG 0.643899 1_3:+57143474 Msa0127660:CDS
CTAGTTCTGGTGAATCAGGT+GGG 0.646582 1_3:-57142805 None:intergenic
CATTTGACCACAGTAAACAA+TGG 0.650155 1_3:+57143691 Msa0127660:CDS
TATCCAAGTTAACAGAGGAG+AGG 0.652511 1_3:+57144788 Msa0127660:three_prime_UTR
TGAATTAGAGAAGGCCACAG+AGG 0.653557 1_3:+57144481 Msa0127660:three_prime_UTR
GTAAAGGTACAGATAGTACA+AGG 0.666698 1_3:+57145455 Msa0127660:three_prime_UTR
TAACCAAAATCTTGACAAGG+TGG 0.668289 1_3:-57144016 None:intergenic
ACTTCTATATGTCTGCATGG+TGG 0.671243 1_3:+57145192 Msa0127660:three_prime_UTR
ATTGTTACTCCAGACTACAA+AGG 0.682862 1_3:-57144766 None:intergenic
AGATAGTACAAGGTATATAG+CGG 0.684320 1_3:+57145465 Msa0127660:three_prime_UTR
TAATAACCAAAATCTTGACA+AGG 0.687571 1_3:-57144019 None:intergenic
AATCACAGTAAAGAATGCAG+AGG 0.689548 1_3:+57143549 Msa0127660:CDS
AATCACAGTAAAGAAAGCAG+AGG 0.689548 1_3:+57143624 Msa0127660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAAATTTTTAAATTATA+TGG + chr1_3:57144331-57144350 Msa0127660:three_prime_UTR 0.0%
!! GTTAATATTCAAAAAACTTA+TGG - chr1_3:57142404-57142423 None:intergenic 15.0%
!! AAAAACAGAAATAAAATTAG+GGG - chr1_3:57142485-57142504 None:intergenic 15.0%
!! GAAAAACAGAAATAAAATTA+GGG - chr1_3:57142486-57142505 None:intergenic 15.0%
!! AGAAAAACAGAAATAAAATT+AGG - chr1_3:57142487-57142506 None:intergenic 15.0%
!!! CTTATTATATATTTTGTATC+TGG + chr1_3:57142661-57142680 Msa0127660:intron 15.0%
!! TAAATTTCAAGAACATTATA+TGG - chr1_3:57143271-57143290 None:intergenic 15.0%
!! AAAAAAAATTCACAATAGAA+TGG - chr1_3:57143931-57143950 None:intergenic 15.0%
!!! AAAAACTTGTTTAAATTAGT+AGG + chr1_3:57144150-57144169 Msa0127660:three_prime_UTR 15.0%
!! ATTTAATCAAATGAAAACAA+GGG - chr1_3:57144407-57144426 None:intergenic 15.0%
!! AATTTAATCAAATGAAAACA+AGG - chr1_3:57144408-57144427 None:intergenic 15.0%
!!! AAATGAAAACTAAATTTTAC+AGG - chr1_3:57145090-57145109 None:intergenic 15.0%
!!! AATTATCTTTCTAAAGGATT+TGG - chr1_3:57142999-57143018 None:intergenic 20.0%
!!! AAATCCTTTAGAAAGATAAT+TGG + chr1_3:57142998-57143017 Msa0127660:intron 20.0%
!! TAATGTTCTTGAAATTTATC+AGG + chr1_3:57143273-57143292 Msa0127660:intron 20.0%
!! TTTAATCAAATGAAAACAAG+GGG - chr1_3:57144406-57144425 None:intergenic 20.0%
!! ATATAAGTTTGAATTAGAGA+AGG + chr1_3:57144472-57144491 Msa0127660:three_prime_UTR 20.0%
!! CGATACTTTGTATATAATAT+TGG + chr1_3:57144618-57144637 Msa0127660:three_prime_UTR 20.0%
!! TTTCCTAACCTTATTAAAAT+GGG + chr1_3:57144664-57144683 Msa0127660:three_prime_UTR 20.0%
!!! AGTAAATAGTAATGTTTTTG+TGG + chr1_3:57144883-57144902 Msa0127660:intron 20.0%
!!! GTAAATAGTAATGTTTTTGT+GGG + chr1_3:57144884-57144903 Msa0127660:intron 20.0%
!! TAAATTTACATGAAAAAGTG+CGG - chr1_3:57145038-57145057 None:intergenic 20.0%
!!! AAATTTTACAGGCTTAATAA+GGG - chr1_3:57145079-57145098 None:intergenic 20.0%
!!! TAAATTTTACAGGCTTAATA+AGG - chr1_3:57145080-57145099 None:intergenic 20.0%
! TTTATAATCGAAGTTTGTCA+AGG + chr1_3:57142733-57142752 Msa0127660:intron 25.0%
! TATTGAAGAAAATAAGCTGA+AGG + chr1_3:57142769-57142788 Msa0127660:CDS 25.0%
! ACTTCCAATTATCTTTCTAA+AGG - chr1_3:57143005-57143024 None:intergenic 25.0%
! ATTTGAAAATTACCTTGCTA+TGG + chr1_3:57143083-57143102 Msa0127660:intron 25.0%
! ATAATCCAACATATACACAA+AGG - chr1_3:57143218-57143237 None:intergenic 25.0%
! GTAATTGATAAGTAGAAGTT+GGG - chr1_3:57143247-57143266 None:intergenic 25.0%
! TAAAAAACCAGAGCATAATA+AGG + chr1_3:57143300-57143319 Msa0127660:CDS 25.0%
! TTGAAGGTAGAATATGATAT+GGG + chr1_3:57143379-57143398 Msa0127660:CDS 25.0%
!!! TTTGACTCTATTTCTTTTTC+TGG - chr1_3:57143730-57143749 None:intergenic 25.0%
!!! TTTTAACTTCATCTCTTCAA+TGG - chr1_3:57143866-57143885 None:intergenic 25.0%
! AAAAATTCACAATAGAATGG+TGG - chr1_3:57143928-57143947 None:intergenic 25.0%
! TAATAACCAAAATCTTGACA+AGG - chr1_3:57144022-57144041 None:intergenic 25.0%
! ATTGAAAACATATGACCAAA+AGG - chr1_3:57144063-57144082 None:intergenic 25.0%
! GCAAGATAGTGAAATATAAT+TGG - chr1_3:57144235-57144254 None:intergenic 25.0%
! TATATTTCACTATCTTGCTA+TGG + chr1_3:57144237-57144256 Msa0127660:three_prime_UTR 25.0%
! TTAATTATACTTTCAATGCG+TGG + chr1_3:57144307-57144326 Msa0127660:three_prime_UTR 25.0%
!! AGAATCTTTTGGATGTATAT+AGG + chr1_3:57144513-57144532 Msa0127660:three_prime_UTR 25.0%
!! GAATCTTTTGGATGTATATA+GGG + chr1_3:57144514-57144533 Msa0127660:three_prime_UTR 25.0%
!! AATCTTTTGGATGTATATAG+GGG + chr1_3:57144515-57144534 Msa0127660:three_prime_UTR 25.0%
!! ATCGATAAACATTTTCACAT+AGG - chr1_3:57144602-57144621 None:intergenic 25.0%
! GTTTCCTAACCTTATTAAAA+TGG + chr1_3:57144663-57144682 Msa0127660:three_prime_UTR 25.0%
! TTCCTAACCTTATTAAAATG+GGG + chr1_3:57144665-57144684 Msa0127660:three_prime_UTR 25.0%
! TAAGTTCATTTCAACATATG+CGG - chr1_3:57145011-57145030 None:intergenic 25.0%
! ATGTAAATTTAGTCCTCTTT+AGG + chr1_3:57145047-57145066 Msa0127660:intron 25.0%
! TGTAAATTTAGTCCTCTTTA+GGG + chr1_3:57145048-57145067 Msa0127660:intron 25.0%
!! AATTTTACAGGCTTAATAAG+GGG - chr1_3:57145078-57145097 None:intergenic 25.0%
!!! GTTTTCATTTTAGAAACTCA+AGG + chr1_3:57145100-57145119 Msa0127660:intron 25.0%
!! GTTTGTCATATTAGGATTTT+AGG + chr1_3:57145308-57145327 Msa0127660:three_prime_UTR 25.0%
CATGAAAAAGTTCAGATACA+TGG + chr1_3:57142863-57142882 Msa0127660:CDS 30.0%
AACGTCTATGAAAGAAAAAG+AGG - chr1_3:57142976-57142995 None:intergenic 30.0%
CGTAATTGATAAGTAGAAGT+TGG - chr1_3:57143248-57143267 None:intergenic 30.0%
ATATGAACCTTATTATGCTC+TGG - chr1_3:57143310-57143329 None:intergenic 30.0%
GTTGAAGGTAGAATATGATA+TGG + chr1_3:57143378-57143397 Msa0127660:CDS 30.0%
TGAAGGTAGAATATGATATG+GGG + chr1_3:57143380-57143399 Msa0127660:CDS 30.0%
GAAACCACAGAAAAAAATAG+AGG + chr1_3:57143501-57143520 Msa0127660:CDS 30.0%
!!! TGTACCTCTATTTTTTTCTG+TGG - chr1_3:57143508-57143527 None:intergenic 30.0%
TACTGTCTTAATCACTGTAT+CGG - chr1_3:57143761-57143780 None:intergenic 30.0%
TAAGACAGTAAAGAATGTTG+AGG + chr1_3:57143771-57143790 Msa0127660:CDS 30.0%
GAGATGAAGTTAAAACTAGA+TGG + chr1_3:57143871-57143890 Msa0127660:CDS 30.0%
! TTGAGATTTGAGATGTTAGT+TGG + chr1_3:57144084-57144103 Msa0127660:three_prime_UTR 30.0%
!! GCTTGCTGTAAATAACTATT+AGG + chr1_3:57144117-57144136 Msa0127660:three_prime_UTR 30.0%
AACAATATCCAAGTTAACAG+AGG + chr1_3:57144783-57144802 Msa0127660:three_prime_UTR 30.0%
ACTTAAAAATTCCTCCAAAC+TGG - chr1_3:57144937-57144956 None:intergenic 30.0%
!!! GAATTTTTAAGTGCACTTGT+AGG + chr1_3:57144945-57144964 Msa0127660:intron 30.0%
TCAAAATGTTAGTATCTTCC+TGG - chr1_3:57145278-57145297 None:intergenic 30.0%
ATCCTAATATGACAAACTTG+AGG - chr1_3:57145305-57145324 None:intergenic 30.0%
! CAGTGATTTTTCTATACTTG+AGG + chr1_3:57145362-57145381 Msa0127660:three_prime_UTR 30.0%
AGATAGTACAAGGTATATAG+CGG + chr1_3:57145465-57145484 Msa0127660:three_prime_UTR 30.0%
ACTAATCAATTGAGTTCACA+GGG - chr1_3:57145565-57145584 None:intergenic 30.0%
TACTAATCAATTGAGTTCAC+AGG - chr1_3:57145566-57145585 None:intergenic 30.0%
!!! TTAGTAGTTTTGTTGAGTTG+TGG + chr1_3:57145580-57145599 Msa0127660:three_prime_UTR 30.0%
!! CAAGTGCAACTATTTTTCAA+AGG - chr1_3:57145680-57145699 None:intergenic 30.0%
TCAAAAAACTTATGGCTGCA+AGG - chr1_3:57142396-57142415 None:intergenic 35.0%
TAATCGAAGTTTGTCAAGGA+TGG + chr1_3:57142737-57142756 Msa0127660:intron 35.0%
TGAAGAAAATAAGCTGAAGG+TGG + chr1_3:57142772-57142791 Msa0127660:CDS 35.0%
TTCTTTGAGTCTCCATTAAC+AGG - chr1_3:57142833-57142852 None:intergenic 35.0%
GTTAATGGAGACTCAAAGAA+TGG + chr1_3:57142833-57142852 Msa0127660:CDS 35.0%
ATGAAGTTTCGTCTAACCAT+GGG - chr1_3:57142920-57142939 None:intergenic 35.0%
AGACGAAACTTCATGATGTT+TGG + chr1_3:57142926-57142945 Msa0127660:intron 35.0%
GTCTATGAAAGAAAAAGAGG+CGG - chr1_3:57142973-57142992 None:intergenic 35.0%
! TGAATTTTCTTTAGTCCCCT+GGG + chr1_3:57143126-57143145 Msa0127660:intron 35.0%
TTCAAAGAATTTCATCCCAG+GGG - chr1_3:57143144-57143163 None:intergenic 35.0%
GTTCAAAGAATTTCATCCCA+GGG - chr1_3:57143145-57143164 None:intergenic 35.0%
TGTTGCCTTTGTGTATATGT+TGG + chr1_3:57143210-57143229 Msa0127660:intron 35.0%
TCATATACCAGAGCATACTA+AGG + chr1_3:57143324-57143343 Msa0127660:CDS 35.0%
TTGGATGCTATTAAGGATGT+AGG + chr1_3:57143403-57143422 Msa0127660:CDS 35.0%
AATCACAGTAAAGAATGCAG+AGG + chr1_3:57143549-57143568 Msa0127660:CDS 35.0%
!! ATGAAGGTGTCAAAAGATCT+GGG + chr1_3:57143598-57143617 Msa0127660:CDS 35.0%
AATCACAGTAAAGAAAGCAG+AGG + chr1_3:57143624-57143643 Msa0127660:CDS 35.0%
CATTTGACCACAGTAAACAA+TGG + chr1_3:57143691-57143710 Msa0127660:CDS 35.0%
!! AAGAGTTTCAAAGGGTATTG+AGG + chr1_3:57143822-57143841 Msa0127660:CDS 35.0%
ACTTGAGTCAAAGCTAAATG+AGG + chr1_3:57143894-57143913 Msa0127660:CDS 35.0%
TAACCAAAATCTTGACAAGG+TGG - chr1_3:57144019-57144038 None:intergenic 35.0%
! TTTTGTCTTAGCACTCCTTT+TGG + chr1_3:57144045-57144064 Msa0127660:three_prime_UTR 35.0%
TATTTACAGCAAGCGATGAA+GGG - chr1_3:57144111-57144130 None:intergenic 35.0%
TTATTTACAGCAAGCGATGA+AGG - chr1_3:57144112-57144131 None:intergenic 35.0%
AAATGAAAACAAGGGGAAAG+TGG - chr1_3:57144399-57144418 None:intergenic 35.0%
ACATCCAAAAGATTCTTTGC+TGG - chr1_3:57144509-57144528 None:intergenic 35.0%
TGATGAAGCAATTGAGCATA+TGG + chr1_3:57144641-57144660 Msa0127660:three_prime_UTR 35.0%
!! CACCCCATTTTAATAAGGTT+AGG - chr1_3:57144670-57144689 None:intergenic 35.0%
CTAACCTTATTAAAATGGGG+TGG + chr1_3:57144668-57144687 Msa0127660:three_prime_UTR 35.0%
!! ACATCCACCCCATTTTAATA+AGG - chr1_3:57144675-57144694 None:intergenic 35.0%
TAGAGATTAGCATGAAAGTC+GGG - chr1_3:57144711-57144730 None:intergenic 35.0%
GTAGAGATTAGCATGAAAGT+CGG - chr1_3:57144712-57144731 None:intergenic 35.0%
! TTTTTGCTTCCTTTGTAGTC+TGG + chr1_3:57144757-57144776 Msa0127660:three_prime_UTR 35.0%
ATTGTTACTCCAGACTACAA+AGG - chr1_3:57144769-57144788 None:intergenic 35.0%
TTCTTGCCATATTACCAGTT+TGG + chr1_3:57144920-57144939 Msa0127660:intron 35.0%
ATTCCTCCAAACTGGTAATA+TGG - chr1_3:57144929-57144948 None:intergenic 35.0%
!! TCATTTTAGAAACTCAAGGC+TGG + chr1_3:57145104-57145123 Msa0127660:intron 35.0%
!!! CTGGCTTGTTTTATATTTGC+AGG + chr1_3:57145123-57145142 Msa0127660:intron 35.0%
! CCAGATATCAAGAGTGTAAA+CGG - chr1_3:57145243-57145262 None:intergenic 35.0%
CGTTTACACTCTTGATATCT+GGG + chr1_3:57145241-57145260 Msa0127660:three_prime_UTR 35.0%
GTTTACACTCTTGATATCTG+GGG + chr1_3:57145242-57145261 Msa0127660:three_prime_UTR 35.0%
TTTACACTCTTGATATCTGG+GGG + chr1_3:57145243-57145262 Msa0127660:three_prime_UTR 35.0%
TGCCTCAAGTTTGTCATATT+AGG + chr1_3:57145300-57145319 Msa0127660:three_prime_UTR 35.0%
ATACAAACGCAACGTGATTA+TGG - chr1_3:57145417-57145436 None:intergenic 35.0%
GCAGAATTTCTCACTTGTAA+AGG + chr1_3:57145439-57145458 Msa0127660:three_prime_UTR 35.0%
! GTAAAGGTACAGATAGTACA+AGG + chr1_3:57145455-57145474 Msa0127660:three_prime_UTR 35.0%
TAGATGACTGATGAGATTAG+TGG + chr1_3:57145493-57145512 Msa0127660:three_prime_UTR 35.0%
TGATGAGATTAGTGGAAAAC+TGG + chr1_3:57145501-57145520 Msa0127660:three_prime_UTR 35.0%
AAGTTCATTCATAGTTCGTC+AGG - chr1_3:57145705-57145724 None:intergenic 35.0%
ACGAACTATGAATGAACTTG+AGG + chr1_3:57145706-57145725 Msa0127660:three_prime_UTR 35.0%
CGAACTATGAATGAACTTGA+GGG + chr1_3:57145707-57145726 Msa0127660:three_prime_UTR 35.0%
CAGTTACAATGCAAGCACAA+CGG + chr1_3:57142528-57142547 Msa0127660:intron 40.0%
AGTTACAATGCAAGCACAAC+GGG + chr1_3:57142529-57142548 Msa0127660:intron 40.0%
GTTCTTAGTCCAAAGCACAT+TGG + chr1_3:57142583-57142602 Msa0127660:intron 40.0%
! TGAAGATGTTACTTCCAGCT+TGG + chr1_3:57142625-57142644 Msa0127660:intron 40.0%
! ATGTTACTTCCAGCTTGGTA+TGG + chr1_3:57142630-57142649 Msa0127660:intron 40.0%
GAAGTATATCCATACCAAGC+TGG - chr1_3:57142642-57142661 None:intergenic 40.0%
CCATTAACAGGAACTAGTTC+TGG - chr1_3:57142821-57142840 None:intergenic 40.0%
CCAGAACTAGTTCCTGTTAA+TGG + chr1_3:57142818-57142837 Msa0127660:CDS 40.0%
CATGAAGTTTCGTCTAACCA+TGG - chr1_3:57142921-57142940 None:intergenic 40.0%
AGTCTTAGTCACACACTCTA+AGG + chr1_3:57143022-57143041 Msa0127660:intron 40.0%
GTCTTAGTCACACACTCTAA+GGG + chr1_3:57143023-57143042 Msa0127660:intron 40.0%
! GTGAATTTTCTTTAGTCCCC+TGG + chr1_3:57143125-57143144 Msa0127660:intron 40.0%
CGTTCAAAGAATTTCATCCC+AGG - chr1_3:57143146-57143165 None:intergenic 40.0%
TGCAGAGATTGAAGAGGATA+TGG + chr1_3:57143354-57143373 Msa0127660:CDS 40.0%
TGAAGAGGATATGGAGTTGA+AGG + chr1_3:57143363-57143382 Msa0127660:CDS 40.0%
GGTAGAATATGATATGGGGT+TGG + chr1_3:57143384-57143403 Msa0127660:CDS 40.0%
TATGGGGTTGGATGCTATTA+AGG + chr1_3:57143396-57143415 Msa0127660:CDS 40.0%
!! GTTGAAAGTGTCAAGAGTTG+AGG + chr1_3:57143447-57143466 Msa0127660:CDS 40.0%
TGAGGAGATGAACTCAGTAA+AGG + chr1_3:57143465-57143484 Msa0127660:CDS 40.0%
GAGGAGATGAACTCAGTAAA+GGG + chr1_3:57143466-57143485 Msa0127660:CDS 40.0%
GGTACAAGTCTCAAAAGATC+TGG + chr1_3:57143522-57143541 Msa0127660:CDS 40.0%
!! GATGAAGGTGTCAAAAGATC+TGG + chr1_3:57143597-57143616 Msa0127660:CDS 40.0%
! TTCAAGTCTGCTTCTTTTCC+AGG - chr1_3:57143658-57143677 None:intergenic 40.0%
GACCACAGTAAACAATGGAA+AGG + chr1_3:57143696-57143715 Msa0127660:CDS 40.0%
TGCCTTTCCATTGTTTACTG+TGG - chr1_3:57143701-57143720 None:intergenic 40.0%
ACTGAAGCCAGAAAAAGAAG+CGG + chr1_3:57143795-57143814 Msa0127660:CDS 40.0%
AAAGCTCCTAACATCCATAC+AGG + chr1_3:57144570-57144589 Msa0127660:three_prime_UTR 40.0%
ATTGAGCCTGTATGGATGTT+AGG - chr1_3:57144579-57144598 None:intergenic 40.0%
TATCCAAGTTAACAGAGGAG+AGG + chr1_3:57144788-57144807 Msa0127660:three_prime_UTR 40.0%
TTGCCATATTACCAGTTTGG+AGG + chr1_3:57144923-57144942 Msa0127660:intron 40.0%
CAGGCAGATCTCATTAATAC+AGG + chr1_3:57145142-57145161 Msa0127660:intron 40.0%
GGTCCAAGAAAAGTTCAAGT+CGG + chr1_3:57145163-57145182 Msa0127660:three_prime_UTR 40.0%
GTCCAAGAAAAGTTCAAGTC+GGG + chr1_3:57145164-57145183 Msa0127660:three_prime_UTR 40.0%
!! AACCCGACTTGAACTTTTCT+TGG - chr1_3:57145169-57145188 None:intergenic 40.0%
CCATGCAGACATATAGAAGT+GGG - chr1_3:57145192-57145211 None:intergenic 40.0%
ACCATGCAGACATATAGAAG+TGG - chr1_3:57145193-57145212 None:intergenic 40.0%
ACTTCTATATGTCTGCATGG+TGG + chr1_3:57145192-57145211 Msa0127660:three_prime_UTR 40.0%
GCACTTATCTGTGTCTTCTT+GGG + chr1_3:57145214-57145233 Msa0127660:three_prime_UTR 40.0%
CCGTTTACACTCTTGATATC+TGG + chr1_3:57145240-57145259 Msa0127660:three_prime_UTR 40.0%
AGTACAAGGTATATAGCGGT+CGG + chr1_3:57145469-57145488 Msa0127660:three_prime_UTR 40.0%
CAATTGAGTTCACAGGGAAA+AGG - chr1_3:57145559-57145578 None:intergenic 40.0%
! ATGAACTTGAGGGTCCTTTT+TGG + chr1_3:57145717-57145736 Msa0127660:three_prime_UTR 40.0%
CGTTGTGCTTGCATTGTAAC+TGG - chr1_3:57142530-57142549 None:intergenic 45.0%
CTTGCACACCAAATCTTCAG+GGG - chr1_3:57142558-57142577 None:intergenic 45.0%
CCTTGCACACCAAATCTTCA+GGG - chr1_3:57142559-57142578 None:intergenic 45.0%
TCCTTGCACACCAAATCTTC+AGG - chr1_3:57142560-57142579 None:intergenic 45.0%
CTGCATCAACCAATGTGCTT+TGG - chr1_3:57142595-57142614 None:intergenic 45.0%
GCTGGAAGTAACATCTTCAG+TGG - chr1_3:57142624-57142643 None:intergenic 45.0%
!! CTAGTTCTGGTGAATCAGGT+GGG - chr1_3:57142808-57142827 None:intergenic 45.0%
!! ACTAGTTCTGGTGAATCAGG+TGG - chr1_3:57142809-57142828 None:intergenic 45.0%
!! GGAACTAGTTCTGGTGAATC+AGG - chr1_3:57142812-57142831 None:intergenic 45.0%
TTCGTCTAACCATGGGTTCA+GGG - chr1_3:57142913-57142932 None:intergenic 45.0%
TTTCGTCTAACCATGGGTTC+AGG - chr1_3:57142914-57142933 None:intergenic 45.0%
AGATCTGCAACTCCATAGCA+AGG - chr1_3:57143098-57143117 None:intergenic 45.0%
TCACGAACCTTAGTATGCTC+TGG - chr1_3:57143334-57143353 None:intergenic 45.0%
TCGTGATGCAGAGATTGAAG+AGG + chr1_3:57143348-57143367 Msa0127660:CDS 45.0%
TTCAACCCTGCTTCTTCTCT+TGG - chr1_3:57143433-57143452 None:intergenic 45.0%
GATGAACTCAGTAAAGGGTG+CGG + chr1_3:57143471-57143490 Msa0127660:CDS 45.0%
AATGCAGAGGAACTGAAACC+AGG + chr1_3:57143562-57143581 Msa0127660:CDS 45.0%
GGAACTGAAACCAGGAAAAG+AGG + chr1_3:57143570-57143589 Msa0127660:CDS 45.0%
! TTCATCTCTGCCTCTTTTCC+TGG - chr1_3:57143583-57143602 None:intergenic 45.0%
AGGAAAAGAGGCAGAGATGA+AGG + chr1_3:57143582-57143601 Msa0127660:CDS 45.0%
AAAGCAGAGGAACTGAAACC+TGG + chr1_3:57143637-57143656 Msa0127660:CDS 45.0%
! CTTGACTCCGCTTCTTTTTC+TGG - chr1_3:57143805-57143824 None:intergenic 45.0%
AGCGGAGTCAAGAGTTTCAA+AGG + chr1_3:57143813-57143832 Msa0127660:CDS 45.0%
GCGGAGTCAAGAGTTTCAAA+GGG + chr1_3:57143814-57143833 Msa0127660:CDS 45.0%
TATTGAGGAGCTGAAACTGC+TGG + chr1_3:57143837-57143856 Msa0127660:CDS 45.0%
! ACAGTAACTTGCGACTTGCT+AGG - chr1_3:57143984-57144003 None:intergenic 45.0%
! CTGCCACCTTGTCAAGATTT+TGG + chr1_3:57144013-57144032 Msa0127660:three_prime_UTR 45.0%
AGGGGAAAGTGGTGTGTATA+AGG - chr1_3:57144388-57144407 None:intergenic 45.0%
TGAATTAGAGAAGGCCACAG+AGG + chr1_3:57144481-57144500 Msa0127660:three_prime_UTR 45.0%
! GGAGCCAGCAAAGAATCTTT+TGG + chr1_3:57144502-57144521 Msa0127660:three_prime_UTR 45.0%
CACATAGGATTGAGCCTGTA+TGG - chr1_3:57144587-57144606 None:intergenic 45.0%
GAGGAGGTTAAGCAATCAAG+AGG + chr1_3:57144807-57144826 Msa0127660:three_prime_UTR 45.0%
ATAAGGGGACATCCCTAAAG+AGG - chr1_3:57145063-57145082 None:intergenic 45.0%
CCCACTTCTATATGTCTGCA+TGG + chr1_3:57145189-57145208 Msa0127660:three_prime_UTR 45.0%
GGCACTTATCTGTGTCTTCT+TGG + chr1_3:57145213-57145232 Msa0127660:three_prime_UTR 45.0%
AATGCTCCATCTGATCTGTC+TGG + chr1_3:57145390-57145409 Msa0127660:three_prime_UTR 45.0%
TATGGACCAGACAGATCAGA+TGG - chr1_3:57145399-57145418 None:intergenic 45.0%
GTACAAGGTATATAGCGGTC+GGG + chr1_3:57145470-57145489 Msa0127660:three_prime_UTR 45.0%
!! CCCTGAAGATTTGGTGTGCA+AGG + chr1_3:57142556-57142575 Msa0127660:intron 50.0%
TCGTCTAACCATGGGTTCAG+GGG - chr1_3:57142912-57142931 None:intergenic 50.0%
ACACAAAGGCAACATCAGCG+AGG - chr1_3:57143204-57143223 None:intergenic 50.0%
GAGAGCCAAGAGAAGAAGCA+GGG + chr1_3:57143425-57143444 Msa0127660:CDS 50.0%
!! ATTCTTTGCTGGCTCCTCTG+TGG - chr1_3:57144498-57144517 None:intergenic 50.0%
CCTCCTCTCCTCTGTTAACT+TGG - chr1_3:57144794-57144813 None:intergenic 50.0%
CCAAGTTAACAGAGGAGAGG+AGG + chr1_3:57144791-57144810 Msa0127660:three_prime_UTR 50.0%
ACGGGTAGCCCCTGAAGATT+TGG + chr1_3:57142547-57142566 Msa0127660:intron 55.0%
AATCCAGTCCCCTGAACCCA+TGG + chr1_3:57142901-57142920 Msa0127660:CDS 55.0%
TAACCATGGGTTCAGGGGAC+TGG - chr1_3:57142907-57142926 None:intergenic 55.0%
GGAGAGCCAAGAGAAGAAGC+AGG + chr1_3:57143424-57143443 Msa0127660:CDS 55.0%
! GAACTCAGTAAAGGGTGCGG+AGG + chr1_3:57143474-57143493 Msa0127660:CDS 55.0%
ATCTGGGGGATCAGTCGTCC+AGG + chr1_3:57145257-57145276 Msa0127660:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 57142318 57145746 57142318 ID=Msa0127660;Name=Msa0127660
chr1_3 mRNA 57142318 57145746 57142318 ID=Msa0127660-mRNA-1;Parent=Msa0127660;Name=Msa0127660-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0.66|0.75|0.75|1|0.75|4|1449|298
chr1_3 exon 57142318 57142326 57142318 ID=Msa0127660-mRNA-1:exon:18164;Parent=Msa0127660-mRNA-1
chr1_3 exon 57142746 57142922 57142746 ID=Msa0127660-mRNA-1:exon:18165;Parent=Msa0127660-mRNA-1
chr1_3 exon 57143295 57144852 57143295 ID=Msa0127660-mRNA-1:exon:18166;Parent=Msa0127660-mRNA-1
chr1_3 exon 57145145 57145746 57145145 ID=Msa0127660-mRNA-1:exon:18167;Parent=Msa0127660-mRNA-1
chr1_3 CDS 57142318 57142326 57142318 ID=Msa0127660-mRNA-1:cds;Parent=Msa0127660-mRNA-1
chr1_3 CDS 57142746 57142922 57142746 ID=Msa0127660-mRNA-1:cds;Parent=Msa0127660-mRNA-1
chr1_3 CDS 57143295 57144005 57143295 ID=Msa0127660-mRNA-1:cds;Parent=Msa0127660-mRNA-1
chr1_3 three_prime_UTR 57144006 57144852 57144006 ID=Msa0127660-mRNA-1:three_prime_utr;Parent=Msa0127660-mRNA-1
chr1_3 three_prime_UTR 57145145 57145746 57145145 ID=Msa0127660-mRNA-1:three_prime_utr;Parent=Msa0127660-mRNA-1
Gene Sequence

>Msa0127660

ATGTTAGGGTTTGTCAAGGATGGACTGCGATATATTGAAGAAAATAAGCTGAAGGTGGCTCTGATTAGCCCACCTGATTCACCAGAACTAGTTCCTGTTAATGGAGACTCAAAGAATGGATTTCATCATGAAAAAGTTCAGATACATGGCAAAGACGAAATTGAAATCCAGTCCCCTGAACCCATGGTTCTTAAAAAACCAGAGCATAATAAGGTTCATATACCAGAGCATACTAAGGTTCGTGATGCAGAGATTGAAGAGGATATGGAGTTGAAGGTAGAATATGATATGGGGTTGGATGCTATTAAGGATGTAGGAGAGCCAAGAGAAGAAGCAGGGTTGAAAGTGTCAAGAGTTGAGGAGATGAACTCAGTAAAGGGTGCGGAGGAACTGAAACCACAGAAAAAAATAGAGGTACAAGTCTCAAAAGATCTGGATTCAATCACAGTAAAGAATGCAGAGGAACTGAAACCAGGAAAAGAGGCAGAGATGAAGGTGTCAAAAGATCTGGGTTCAATCACAGTAAAGAAAGCAGAGGAACTGAAACCTGGAAAAGAAGCAGACTTGAATGTGTTAAAAGATCATTTGACCACAGTAAACAATGGAAAGGCACTGACGCCAGAAAAAGAAATAGAGTCAAAAGTGTCAACCGATACAGTGATTAAGACAGTAAAGAATGTTGAGGAACTGAAGCCAGAAAAAGAAGCGGAGTCAAGAGTTTCAAAGGGTATTGAGGAGCTGAAACTGCTGGAAGCCATTGAAGAGATGAAGTTAAAACTAGATGGACTTGAGTCAAAGCTAAATGAGGTCAGCATTCCACCATTCTATTGTGAATTTTTTTTTATCTGCTTGCTGTTACATGTGACATGCTTCCTAGCAAGTCGCAAGTTACTGTAA
Protein sequence

>Msa0127660

MLGFVKDGLRYIEENKLKVALISPPDSPELVPVNGDSKNGFHHEKVQIHGKDEIEIQSPEPMVLKKPEHNKVHIPEHTKVRDAEIEEDMELKVEYDMGLDAIKDVGEPREEAGLKVSRVEEMNSVKGAEELKPQKKIEVQVSKDLDSITVKNAEELKPGKEAEMKVSKDLGSITVKKAEELKPGKEADLNVLKDHLTTVNNGKALTPEKEIESKVSTDTVIKTVKNVEELKPEKEAESRVSKGIEELKLLEAIEEMKLKLDGLESKLNEVSIPPFYCEFFFICLLLHVTCFLASRKLL*