Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0341910 | XP_003597750.1 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 635 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0341910 | G7II20 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 635 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001590 | Msa0341910 | 0.809215 | 2.112521e-50 | -8.615850e-47 |
| Msa0017510 | Msa0341910 | 0.821622 | 3.661120e-53 | -8.615850e-47 |
| Msa0034270 | Msa0341910 | 0.809368 | 1.958992e-50 | -8.615850e-47 |
| Msa0046100 | Msa0341910 | 0.813142 | 2.972953e-51 | -8.615850e-47 |
| Msa0093400 | Msa0341910 | 0.988191 | 4.619859e-173 | -8.615850e-47 |
| Msa0094850 | Msa0341910 | 0.831085 | 2.038541e-55 | -8.615850e-47 |
| Msa0108160 | Msa0341910 | 0.827543 | 1.476444e-54 | -8.615850e-47 |
| Msa0110970 | Msa0341910 | 0.811870 | 5.638199e-51 | -8.615850e-47 |
| Msa0127660 | Msa0341910 | 0.805017 | 1.635164e-49 | -8.615850e-47 |
| Msa0152400 | Msa0341910 | 0.801729 | 7.849098e-49 | -8.615850e-47 |
| Msa0152430 | Msa0341910 | 0.809612 | 1.736457e-50 | -8.615850e-47 |
| Msa0157290 | Msa0341910 | 0.821652 | 3.602332e-53 | -8.615850e-47 |
| Msa0165430 | Msa0341910 | 0.801898 | 7.246203e-49 | -8.615850e-47 |
| Msa0190690 | Msa0341910 | 0.809087 | 2.250647e-50 | -8.615850e-47 |
| Msa0211150 | Msa0341910 | 0.804086 | 2.556741e-49 | -8.615850e-47 |
| Msa0212990 | Msa0341910 | 0.808377 | 3.190639e-50 | -8.615850e-47 |
| Msa0225320 | Msa0341910 | 0.937300 | 4.331076e-98 | -8.615850e-47 |
| Msa0251050 | Msa0341910 | 0.818733 | 1.680562e-52 | -8.615850e-47 |
| Msa0297740 | Msa0341910 | 0.801911 | 7.199846e-49 | -8.615850e-47 |
| Msa0306300 | Msa0341910 | 0.831452 | 1.656487e-55 | -8.615850e-47 |
| Msa0311420 | Msa0341910 | 0.814742 | 1.319234e-51 | -8.615850e-47 |
| Msa0330340 | Msa0341910 | 0.800484 | 1.410183e-48 | -8.615850e-47 |
| Msa0334220 | Msa0341910 | 0.826168 | 3.144691e-54 | -8.615850e-47 |
| Msa0335040 | Msa0341910 | 0.807354 | 5.265271e-50 | -8.615850e-47 |
| Msa0337330 | Msa0341910 | 0.802661 | 5.046551e-49 | -8.615850e-47 |
| Msa0341910 | Msa0363350 | 0.805297 | 1.429153e-49 | -8.615850e-47 |
| Msa0341910 | Msa0369850 | 0.809775 | 1.601299e-50 | -8.615850e-47 |
| Msa0341910 | Msa0389340 | 0.812070 | 5.101286e-51 | -8.615850e-47 |
| Msa0341910 | Msa0417270 | 0.804709 | 1.896869e-49 | -8.615850e-47 |
| Msa0341910 | Msa0417880 | 0.815899 | 7.297997e-52 | -8.615850e-47 |
| Msa0341910 | Msa0452920 | 0.802751 | 4.836111e-49 | -8.615850e-47 |
| Msa0341910 | Msa0452960 | 0.828691 | 7.807944e-55 | -8.615850e-47 |
| Msa0341910 | Msa0457470 | 0.800503 | 1.397738e-48 | -8.615850e-47 |
| Msa0341910 | Msa0461730 | 0.815490 | 9.001714e-52 | -8.615850e-47 |
| Msa0341910 | Msa0467430 | 0.800365 | 1.491278e-48 | -8.615850e-47 |
| Msa0341910 | Msa0469160 | 0.815633 | 8.366208e-52 | -8.615850e-47 |
| Msa0341910 | Msa0484780 | 0.803371 | 3.599803e-49 | -8.615850e-47 |
| Msa0341910 | Msa0495320 | 0.829992 | 3.773717e-55 | -8.615850e-47 |
| Msa0341910 | Msa0498060 | 0.805943 | 1.045782e-49 | -8.615850e-47 |
| Msa0341910 | Msa0507680 | 0.814967 | 1.176391e-51 | -8.615850e-47 |
| Msa0341910 | Msa0509610 | 0.817246 | 3.641663e-52 | -8.615850e-47 |
| Msa0341910 | Msa0513160 | 0.815614 | 8.446587e-52 | -8.615850e-47 |
| Msa0341910 | Msa0545210 | 0.813683 | 2.260296e-51 | -8.615850e-47 |
| Msa0341910 | Msa0546680 | 0.815105 | 1.096490e-51 | -8.615850e-47 |
| Msa0341910 | Msa0616160 | 0.810099 | 1.363946e-50 | -8.615850e-47 |
| Msa0341910 | Msa0626450 | 0.820525 | 6.551303e-53 | -8.615850e-47 |
| Msa0341910 | Msa0626490 | 0.822705 | 2.053296e-53 | -8.615850e-47 |
| Msa0341910 | Msa0633700 | 0.810259 | 1.260078e-50 | -8.615850e-47 |
| Msa0341910 | Msa0655560 | 0.800238 | 1.582563e-48 | -8.615850e-47 |
| Msa0341910 | Msa0664600 | 0.825046 | 5.801795e-54 | -8.615850e-47 |
| Msa0341910 | Msa0682550 | 0.804028 | 2.629155e-49 | -8.615850e-47 |
| Msa0341910 | Msa0693380 | 0.809848 | 1.544786e-50 | -8.615850e-47 |
| Msa0341910 | Msa0705870 | 0.805453 | 1.325121e-49 | -8.615850e-47 |
| Msa0341910 | Msa0708520 | 0.800050 | 1.728565e-48 | -8.615850e-47 |
| Msa0341910 | Msa0712590 | 0.811355 | 7.295902e-51 | -8.615850e-47 |
| Msa0341910 | Msa0759120 | 0.810288 | 1.241790e-50 | -8.615850e-47 |
| Msa0341910 | Msa0767300 | 0.805846 | 1.096036e-49 | -8.615850e-47 |
| Msa0341910 | Msa0795390 | 0.810129 | 1.343872e-50 | -8.615850e-47 |
| Msa0341910 | Msa0824660 | 0.802199 | 6.282963e-49 | -8.615850e-47 |
| Msa0341910 | Msa0844240 | 0.818041 | 2.410144e-52 | -8.615850e-47 |
| Msa0341910 | Msa0870530 | 0.812725 | 3.668785e-51 | -8.615850e-47 |
| Msa0341910 | Msa0875290 | 0.802127 | 6.501995e-49 | -8.615850e-47 |
| Msa0341910 | Msa0880140 | 0.820515 | 6.584001e-53 | -8.615850e-47 |
| Msa0341910 | Msa0903700 | 0.819313 | 1.240152e-52 | -8.615850e-47 |
| Msa0341910 | Msa0909120 | 0.821826 | 3.283719e-53 | -8.615850e-47 |
| Msa0341910 | Msa0973150 | 0.816979 | 4.183358e-52 | -8.615850e-47 |
| Msa0341910 | Msa0996680 | 0.822826 | 1.924062e-53 | -8.615850e-47 |
| Msa0341910 | Msa1007270 | 0.803412 | 3.529846e-49 | -8.615850e-47 |
| Msa0341910 | Msa1024740 | 0.813190 | 2.902096e-51 | -8.615850e-47 |
| Msa0341910 | Msa1031930 | 0.829285 | 5.607054e-55 | -8.615850e-47 |
| Msa0341910 | Msa1043920 | 0.830715 | 2.511684e-55 | -8.615850e-47 |
| Msa0341910 | Msa1082500 | 0.820630 | 6.197933e-53 | -8.615850e-47 |
| Msa0341910 | Msa1085200 | 0.801338 | 9.437427e-49 | -8.615850e-47 |
| Msa0341910 | Msa1085300 | 0.800038 | 1.738044e-48 | -8.615850e-47 |
| Msa0341910 | Msa1104000 | 0.815347 | 9.686882e-52 | -8.615850e-47 |
| Msa0341910 | Msa1108160 | 0.806317 | 8.723705e-50 | -8.615850e-47 |
| Msa0341910 | Msa1108790 | 0.825036 | 5.833624e-54 | -8.615850e-47 |
| Msa0341910 | Msa1151910 | 0.823447 | 1.378547e-53 | -8.615850e-47 |
| Msa0341910 | Msa1152510 | 0.817692 | 2.890956e-52 | -8.615850e-47 |
| Msa0341910 | Msa1179940 | 0.814852 | 1.247294e-51 | -8.615850e-47 |
| Msa0341910 | Msa1179970 | 0.814894 | 1.221107e-51 | -8.615850e-47 |
| Msa0341910 | Msa1182860 | 0.813387 | 2.626495e-51 | -8.615850e-47 |
| Msa0341910 | Msa1183120 | 0.823856 | 1.105753e-53 | -8.615850e-47 |
| Msa0341910 | Msa1188860 | 0.800724 | 1.260260e-48 | -8.615850e-47 |
| Msa0341910 | Msa1205290 | 0.817111 | 3.905583e-52 | -8.615850e-47 |
| Msa0341910 | Msa1211150 | -0.835123 | 2.014986e-56 | -8.615850e-47 |
| Msa0341910 | Msa1229500 | 0.828065 | 1.105523e-54 | -8.615850e-47 |
| Msa0341910 | Msa1236100 | 0.806908 | 6.546558e-50 | -8.615850e-47 |
| Msa0341910 | Msa1242220 | 0.812242 | 4.678339e-51 | -8.615850e-47 |
| Msa0341910 | Msa1270610 | 0.836153 | 1.105362e-56 | -8.615850e-47 |
| Msa0341910 | Msa1271300 | 0.800598 | 1.336859e-48 | -8.615850e-47 |
| Msa0341910 | Msa1271990 | 0.841435 | 4.761595e-58 | -8.615850e-47 |
| Msa0341910 | Msa1272900 | 0.813679 | 2.264951e-51 | -8.615850e-47 |
| Msa0341910 | Msa1283840 | 0.808818 | 2.569553e-50 | -8.615850e-47 |
| Msa0341910 | Msa1283860 | 0.815781 | 7.752343e-52 | -8.615850e-47 |
| Msa0341910 | Msa1284260 | 0.803262 | 3.790663e-49 | -8.615850e-47 |
| Msa0341910 | Msa1299300 | -0.812467 | 4.178395e-51 | -8.615850e-47 |
| Msa0341910 | Msa1308380 | 0.818091 | 2.348714e-52 | -8.615850e-47 |
| Msa0341910 | Msa1322890 | 0.826621 | 2.453083e-54 | -8.615850e-47 |
| Msa0341910 | Msa1327050 | 0.819771 | 9.747778e-53 | -8.615850e-47 |
| Msa0341910 | Msa1355800 | 0.998292 | 5.209057e-261 | -8.615850e-47 |
| Msa0341910 | Msa1384350 | 0.806793 | 6.920796e-50 | -8.615850e-47 |
| Msa0341910 | Msa1414740 | 0.804079 | 2.566295e-49 | -8.615850e-47 |
| Msa0341910 | Msa1431430 | 0.811605 | 6.438447e-51 | -8.615850e-47 |
| Msa0341910 | Msa1440960 | 0.816231 | 6.151002e-52 | -8.615850e-47 |
| Msa0341910 | Msa1445190 | 0.815210 | 1.038880e-51 | -8.615850e-47 |
| Msa0341910 | Msa1452120 | 0.813695 | 2.247642e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0341910 | MtrunA17_Chr2g0331671 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 635 |
| Msa0341910 | MtrunA17_Chr6g0486001 | 52.885 | 312 | 135 | 6 | 2 | 308 | 1 | 305 | 4.85e-103 | 303 |
| Msa0341910 | MtrunA17_Chr6g0483441 | 38.699 | 292 | 153 | 5 | 22 | 309 | 25 | 294 | 9.74e-62 | 197 |
| Msa0341910 | MtrunA17_Chr8g0392551 | 41.667 | 312 | 162 | 7 | 2 | 309 | 1 | 296 | 9.92e-61 | 195 |
| Msa0341910 | MtrunA17_Chr4g0053001 | 40.994 | 322 | 169 | 9 | 4 | 309 | 14 | 330 | 1.15e-59 | 194 |
| Msa0341910 | MtrunA17_Chr4g0038691 | 36.050 | 319 | 177 | 10 | 6 | 309 | 12 | 318 | 1.35e-49 | 167 |
| Msa0341910 | MtrunA17_Chr3g0091821 | 31.138 | 167 | 99 | 4 | 128 | 293 | 26 | 177 | 2.93e-18 | 81.3 |
| Msa0341910 | MtrunA17_Chr8g0379281 | 37.755 | 98 | 55 | 2 | 137 | 234 | 106 | 197 | 6.43e-16 | 75.1 |
| Msa0341910 | MtrunA17_Chr3g0090061 | 22.977 | 309 | 198 | 8 | 27 | 297 | 29 | 335 | 9.45e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0341910 | AT1G45688.1 | 43.614 | 321 | 147 | 7 | 2 | 289 | 1 | 320 | 1.69e-69 | 219 |
| Msa0341910 | AT5G42860.1 | 40.645 | 310 | 146 | 5 | 2 | 287 | 1 | 296 | 3.01e-65 | 208 |
| Msa0341910 | AT3G24605.1 | 40.129 | 309 | 160 | 7 | 5 | 306 | 6 | 296 | 3.63e-64 | 204 |
| Msa0341910 | AT3G24600.1 | 41.613 | 310 | 168 | 9 | 1 | 306 | 1 | 301 | 1.63e-56 | 185 |
| Msa0341910 | AT1G45688.2 | 45.382 | 249 | 119 | 8 | 2 | 235 | 1 | 247 | 2.67e-52 | 172 |
| Msa0341910 | AT4G35170.1 | 35.789 | 285 | 159 | 9 | 14 | 287 | 6 | 277 | 2.39e-42 | 148 |
| Msa0341910 | AT2G41990.1 | 35.117 | 299 | 170 | 8 | 2 | 293 | 1 | 282 | 1.97e-39 | 140 |
| Msa0341910 | AT3G08490.1 | 22.932 | 266 | 179 | 4 | 27 | 291 | 11 | 251 | 1.82e-13 | 69.7 |
Find 78 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAGTGCTTTGAAATTAAA+AGG | 0.150017 | 2_4:+63835414 | None:intergenic |
| GGTAATAAGATTCCCCTTTA+TGG | 0.167603 | 2_4:-63833472 | Msa0341910:CDS |
| GAATTCTTCTTGAATTTAAT+AGG | 0.271825 | 2_4:+63833286 | None:intergenic |
| TCTCCTTCACATCCTTCTTT+TGG | 0.272863 | 2_4:-63834877 | Msa0341910:CDS |
| TCCATAGGACTGTTTGATAT+TGG | 0.301627 | 2_4:+63834901 | None:intergenic |
| AGTAGAAAGAGGAATGATAA+AGG | 0.322695 | 2_4:-63834784 | Msa0341910:CDS |
| ACAATACGGTGACTCTTTCT+TGG | 0.322732 | 2_4:+63833511 | None:intergenic |
| ACATAGTATACAGGTCTCTT+TGG | 0.336582 | 2_4:+63834982 | None:intergenic |
| AATGGAAATAAATGAGATAA+TGG | 0.364937 | 2_4:+63835325 | None:intergenic |
| GGGTGCTAGCAGGCCTTTCA+AGG | 0.389621 | 2_4:-63834623 | Msa0341910:CDS |
| AGGTGATGTGGTGGGGAAAC+TGG | 0.391294 | 2_4:-63833371 | Msa0341910:CDS |
| TAAGGAAATCACTTGTCCTT+TGG | 0.400348 | 2_4:-63833332 | Msa0341910:CDS |
| TAACAAGAAACTCAAAGAAA+TGG | 0.408295 | 2_4:+63835307 | None:intergenic |
| GGTCTCTTTGGAGATCTTGA+TGG | 0.410924 | 2_4:+63834994 | None:intergenic |
| CCTTTGGTTATCAATTCTGC+TGG | 0.414114 | 2_4:-63833316 | Msa0341910:CDS |
| CCATCATGTGAATCTCTTGA+AGG | 0.414191 | 2_4:+63834952 | None:intergenic |
| TTTCAGCAGCCAGTGATGTT+TGG | 0.414699 | 2_4:-63835491 | Msa0341910:five_prime_UTR |
| CAGTACATAATCTATATGTT+TGG | 0.415741 | 2_4:-63833846 | Msa0341910:CDS |
| TCAGAAACTTCTCTTGCAAA+TGG | 0.415938 | 2_4:+63833148 | None:intergenic |
| TGATCATTTGGGGTGCTAGC+AGG | 0.416591 | 2_4:-63834633 | Msa0341910:CDS |
| AGTACATAATCTATATGTTT+GGG | 0.418371 | 2_4:-63833845 | Msa0341910:CDS |
| TCCGGTTTGTGAAACTGTTA+TGG | 0.424945 | 2_4:+63833438 | None:intergenic |
| GTTTGCACTTTGCAAGGAAA+TGG | 0.429304 | 2_4:+63835366 | None:intergenic |
| TGGTTGATCCAGCTCCATAA+AGG | 0.434697 | 2_4:+63833458 | None:intergenic |
| AAGATTCCCCTTTATGGAGC+TGG | 0.446069 | 2_4:-63833466 | Msa0341910:CDS |
| GGTTGATCCAGCTCCATAAA+GGG | 0.452029 | 2_4:+63833459 | None:intergenic |
| ACCATAACAGTTTCACAAAC+CGG | 0.452618 | 2_4:-63833439 | Msa0341910:CDS |
| AAGTGCTACTTCAACACTTC+CGG | 0.453891 | 2_4:+63833420 | None:intergenic |
| CCGTATTGTATCAGTGAACT+TGG | 0.454362 | 2_4:-63833497 | Msa0341910:CDS |
| TCCAATATCAAACAGTCCTA+TGG | 0.455908 | 2_4:-63834902 | Msa0341910:CDS |
| AGGCTTGGAACAAAACATAA+AGG | 0.468103 | 2_4:+63835459 | None:intergenic |
| GGAGATCTTGATGGTGAAGA+TGG | 0.477710 | 2_4:+63835003 | None:intergenic |
| GTAGAAAGAGGAATGATAAA+GGG | 0.480419 | 2_4:-63834783 | Msa0341910:CDS |
| GGTAAGGACAAGGCATCATA+AGG | 0.484890 | 2_4:-63833350 | Msa0341910:CDS |
| CTAGTGCTTTGAAATTAAAA+GGG | 0.488026 | 2_4:+63835415 | None:intergenic |
| GGACTTTGTACATAGTATAC+AGG | 0.503085 | 2_4:+63834973 | None:intergenic |
| CTCGAGTGACGGCCAAAAGA+AGG | 0.504004 | 2_4:+63834865 | None:intergenic |
| CCTTCAAGAGATTCACATGA+TGG | 0.519036 | 2_4:-63834952 | Msa0341910:CDS |
| GAAGGAAAGGTAGTAGAAAG+AGG | 0.523236 | 2_4:-63834795 | Msa0341910:CDS |
| ACAGTAACTTCTGCCTTGAA+AGG | 0.531838 | 2_4:+63834610 | None:intergenic |
| AAGTAAAGTAAGTGATTGAA+AGG | 0.534784 | 2_4:+63835439 | None:intergenic |
| TCAGATATCAGTATCGCAAC+CGG | 0.536332 | 2_4:-63833694 | Msa0341910:CDS |
| AAGTAAGTGATTGAAAGGCT+TGG | 0.546484 | 2_4:+63835444 | None:intergenic |
| CCAAGTTCACTGATACAATA+CGG | 0.554928 | 2_4:+63833497 | None:intergenic |
| CAAGGCAGAAGTTACTGTCA+AGG | 0.562919 | 2_4:-63834605 | Msa0341910:intron |
| GATCGCGAGGTGATGTGGTG+GGG | 0.563802 | 2_4:-63833378 | Msa0341910:CDS |
| CGATCGCGAGGTGATGTGGT+GGG | 0.564068 | 2_4:-63833379 | Msa0341910:CDS |
| TGATCGGTGAAGTGTGGACA+TGG | 0.570257 | 2_4:+63833728 | None:intergenic |
| AGGTCATCTTCAGGAAGGAA+AGG | 0.572600 | 2_4:-63834808 | Msa0341910:CDS |
| CGAGAAACTCTTCTGCTAGT+CGG | 0.574468 | 2_4:-63834846 | Msa0341910:CDS |
| TAAGAATGATCTTACCTCAC+CGG | 0.575350 | 2_4:+63833675 | None:intergenic |
| ATGTGGTGGGGAAACTGGTA+AGG | 0.575803 | 2_4:-63833366 | Msa0341910:CDS |
| TGTGGTGTTATACTTGAAGA+AGG | 0.580795 | 2_4:-63834751 | Msa0341910:CDS |
| GAGTTTGTTTGCACTTTGCA+AGG | 0.586534 | 2_4:+63835360 | None:intergenic |
| TCTGAGTAAACAAGATTGAT+CGG | 0.590368 | 2_4:+63833712 | None:intergenic |
| CTTTAAGAACCAAACATCAC+TGG | 0.592949 | 2_4:+63835482 | None:intergenic |
| CAGAAGAGTTTCTCGAGTGA+CGG | 0.597006 | 2_4:+63834854 | None:intergenic |
| AATCCGATCGCGAGGTGATG+TGG | 0.597666 | 2_4:-63833383 | Msa0341910:CDS |
| ATTGTATCAGTGAACTTGGA+AGG | 0.600518 | 2_4:-63833493 | Msa0341910:CDS |
| GGATGTGAAGGAGACTCCAT+AGG | 0.604242 | 2_4:+63834886 | None:intergenic |
| CGGCCAAAAGAAGGATGTGA+AGG | 0.605614 | 2_4:+63834874 | None:intergenic |
| ACATAATCTATATGTTTGGG+AGG | 0.606954 | 2_4:-63833842 | Msa0341910:CDS |
| TGATATTGGAGTAGCTTGCA+TGG | 0.609840 | 2_4:+63834915 | None:intergenic |
| CAAGATTGATCGGTGAAGTG+TGG | 0.610457 | 2_4:+63833722 | None:intergenic |
| CCACCACATCACCTCGCGAT+CGG | 0.610908 | 2_4:+63833380 | None:intergenic |
| AGGTTCTTATCGTGAATTCG+AGG | 0.611273 | 2_4:-63834731 | Msa0341910:CDS |
| CCGATCGCGAGGTGATGTGG+TGG | 0.611667 | 2_4:-63833380 | Msa0341910:CDS |
| TGGGGAAACTGGTAAGGACA+AGG | 0.623482 | 2_4:-63833360 | Msa0341910:CDS |
| CCAGCAGAATTGATAACCAA+AGG | 0.632275 | 2_4:+63833316 | None:intergenic |
| AAGTTTGAAATCCGATCGCG+AGG | 0.634220 | 2_4:-63833391 | Msa0341910:CDS |
| GTTGATCCAGCTCCATAAAG+GGG | 0.651471 | 2_4:+63833460 | None:intergenic |
| TCAAGTATAACACCACACTC+AGG | 0.656313 | 2_4:+63834757 | None:intergenic |
| GATAAAGGGTGGCCTGAGTG+TGG | 0.658555 | 2_4:-63834769 | Msa0341910:CDS |
| CATAATCTATATGTTTGGGA+GGG | 0.659908 | 2_4:-63833841 | Msa0341910:CDS |
| TATCAGTATCGCAACCGGTG+AGG | 0.671558 | 2_4:-63833689 | Msa0341910:intron |
| GAAAGAGGAATGATAAAGGG+TGG | 0.678134 | 2_4:-63834780 | Msa0341910:CDS |
| TCACTGGCTGCTGAAAGTGT+CGG | 0.729310 | 2_4:+63835498 | None:intergenic |
| TGGGAGGGTACAGACCACAC+AGG | 0.759316 | 2_4:-63833826 | Msa0341910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACTATTATAAAATCAGTT+TGG | - | chr2_4:63834115-63834134 | Msa0341910:intron | 15.0% |
| !! | AATGGAAATAAATGAGATAA+TGG | + | chr2_4:63833205-63833224 | None:intergenic | 20.0% |
| !! | AGACTTAAGAATAACAAAAT+TGG | + | chr2_4:63833951-63833970 | None:intergenic | 20.0% |
| !! | AACTTAAGCTTAATGTATAA+AGG | + | chr2_4:63834183-63834202 | None:intergenic | 20.0% |
| !!! | AATAGTTTAACTTTTCTTTG+TGG | - | chr2_4:63834222-63834241 | Msa0341910:intron | 20.0% |
| !!! | AACTGTATTTTTAACTTTCA+AGG | + | chr2_4:63834389-63834408 | None:intergenic | 20.0% |
| !! | TTGAAAGTTAAAAATACAGT+TGG | - | chr2_4:63834388-63834407 | Msa0341910:intron | 20.0% |
| !! | AGAATAATATAGAAACATAC+AGG | + | chr2_4:63834495-63834514 | None:intergenic | 20.0% |
| !!! | AATTTTGTTTCAATCACTTT+GGG | - | chr2_4:63834536-63834555 | Msa0341910:intron | 20.0% |
| !! | TTAATTAATAATGCAGAGTT+TGG | - | chr2_4:63834658-63834677 | Msa0341910:CDS | 20.0% |
| !! | AGTACATAATCTATATGTTT+GGG | - | chr2_4:63834682-63834701 | Msa0341910:CDS | 20.0% |
| !!! | TAATTGTCAGAATTTTTGTT+GGG | + | chr2_4:63834730-63834749 | None:intergenic | 20.0% |
| !!! | TTAATTGTCAGAATTTTTGT+TGG | + | chr2_4:63834731-63834750 | None:intergenic | 20.0% |
| !!! | AATTCAAGTAACTTTTTTCT+TGG | - | chr2_4:63834931-63834950 | Msa0341910:CDS | 20.0% |
| !! | GAATTCTTCTTGAATTTAAT+AGG | + | chr2_4:63835244-63835263 | None:intergenic | 20.0% |
| ! | AAGTAAAGTAAGTGATTGAA+AGG | + | chr2_4:63833091-63833110 | None:intergenic | 25.0% |
| !!! | CTAGTGCTTTGAAATTAAAA+GGG | + | chr2_4:63833115-63833134 | None:intergenic | 25.0% |
| ! | TAACAAGAAACTCAAAGAAA+TGG | + | chr2_4:63833223-63833242 | None:intergenic | 25.0% |
| !!! | TGTTTTCTAGTACTATTTAC+TGG | - | chr2_4:63833331-63833350 | Msa0341910:CDS | 25.0% |
| !!! | TGTGTTTTGTTTGATCATTT+GGG | - | chr2_4:63833883-63833902 | Msa0341910:intron | 25.0% |
| ! | TTAATATCAGCCAACTAAAA+TGG | + | chr2_4:63834344-63834363 | None:intergenic | 25.0% |
| ! | CAAAGTGATTGAAACAAAAT+TGG | + | chr2_4:63834537-63834556 | None:intergenic | 25.0% |
| !!! | CAATTTTGTTTCAATCACTT+TGG | - | chr2_4:63834535-63834554 | Msa0341910:intron | 25.0% |
| !!! | TTCTTTTTTGGTACAAAAGA+GGG | + | chr2_4:63834567-63834586 | None:intergenic | 25.0% |
| !!! | TTTCTTTTTTGGTACAAAAG+AGG | + | chr2_4:63834568-63834587 | None:intergenic | 25.0% |
| !! | CTTTTGTACCAAAAAAGAAA+TGG | - | chr2_4:63834568-63834587 | Msa0341910:intron | 25.0% |
| !!! | TTGCTATTTATAATGTTCCT+TGG | - | chr2_4:63834602-63834621 | Msa0341910:intron | 25.0% |
| !! | CAATACTACTTAATTTTCCA+AGG | + | chr2_4:63834622-63834641 | None:intergenic | 25.0% |
| ! | CAGTACATAATCTATATGTT+TGG | - | chr2_4:63834681-63834700 | Msa0341910:CDS | 25.0% |
| !!! | TTTGAGTATTTTTGAGATTG+AGG | - | chr2_4:63835352-63835371 | Msa0341910:five_prime_UTR | 25.0% |
| !! | GCTAGTGCTTTGAAATTAAA+AGG | + | chr2_4:63833116-63833135 | None:intergenic | 30.0% |
| AGTAGAAAGAGGAATGATAA+AGG | - | chr2_4:63833743-63833762 | Msa0341910:CDS | 30.0% | |
| GTAGAAAGAGGAATGATAAA+GGG | - | chr2_4:63833744-63833763 | Msa0341910:CDS | 30.0% | |
| !!! | CTGTGTTTTGTTTGATCATT+TGG | - | chr2_4:63833882-63833901 | Msa0341910:intron | 30.0% |
| !!! | GTGTTTTGTTTGATCATTTG+GGG | - | chr2_4:63833884-63833903 | Msa0341910:intron | 30.0% |
| !! | TTATTTCTACAAGCTCTCTT+GGG | - | chr2_4:63834071-63834090 | Msa0341910:intron | 30.0% |
| AACTAAAATGGATGCATAGT+AGG | + | chr2_4:63834332-63834351 | None:intergenic | 30.0% | |
| !! | TACTATGCATCCATTTTAGT+TGG | - | chr2_4:63834331-63834350 | Msa0341910:intron | 30.0% |
| TGATATTAACACTGACAGAA+AGG | - | chr2_4:63834355-63834374 | Msa0341910:intron | 30.0% | |
| !!! | TGCTACTACCATTTCTTTTT+TGG | + | chr2_4:63834579-63834598 | None:intergenic | 30.0% |
| ACATAATCTATATGTTTGGG+AGG | - | chr2_4:63834685-63834704 | Msa0341910:CDS | 30.0% | |
| CATAATCTATATGTTTGGGA+GGG | - | chr2_4:63834686-63834705 | Msa0341910:CDS | 30.0% | |
| TCTGAGTAAACAAGATTGAT+CGG | + | chr2_4:63834818-63834837 | None:intergenic | 30.0% | |
| ! | CTTTAAGAACCAAACATCAC+TGG | + | chr2_4:63833048-63833067 | None:intergenic | 35.0% |
| AGGCTTGGAACAAAACATAA+AGG | + | chr2_4:63833071-63833090 | None:intergenic | 35.0% | |
| AAGTAAGTGATTGAAAGGCT+TGG | + | chr2_4:63833086-63833105 | None:intergenic | 35.0% | |
| ACATAGTATACAGGTCTCTT+TGG | + | chr2_4:63833548-63833567 | None:intergenic | 35.0% | |
| GGACTTTGTACATAGTATAC+AGG | + | chr2_4:63833557-63833576 | None:intergenic | 35.0% | |
| TCCAATATCAAACAGTCCTA+TGG | - | chr2_4:63833625-63833644 | Msa0341910:intron | 35.0% | |
| TCCATAGGACTGTTTGATAT+TGG | + | chr2_4:63833629-63833648 | None:intergenic | 35.0% | |
| !!! | TTTTTAGGTCATCTTCAGGA+AGG | - | chr2_4:63833714-63833733 | Msa0341910:CDS | 35.0% |
| !! | TGTGGTGTTATACTTGAAGA+AGG | - | chr2_4:63833776-63833795 | Msa0341910:CDS | 35.0% |
| !! | GATTGCTGTGTTTACTTTTC+TGG | - | chr2_4:63833850-63833869 | Msa0341910:CDS | 35.0% |
| !! | CTTATTTCTACAAGCTCTCT+TGG | - | chr2_4:63834070-63834089 | Msa0341910:intron | 35.0% |
| TGATGCCTTCTTCAAAAAGA+TGG | + | chr2_4:63834249-63834268 | None:intergenic | 35.0% | |
| !!! | TACAACCCTGCTACATTTTT+TGG | - | chr2_4:63834773-63834792 | Msa0341910:CDS | 35.0% |
| TAAGAATGATCTTACCTCAC+CGG | + | chr2_4:63834855-63834874 | None:intergenic | 35.0% | |
| CCAAGTTCACTGATACAATA+CGG | + | chr2_4:63835033-63835052 | None:intergenic | 35.0% | |
| ATTGTATCAGTGAACTTGGA+AGG | - | chr2_4:63835034-63835053 | Msa0341910:CDS | 35.0% | |
| GGTAATAAGATTCCCCTTTA+TGG | - | chr2_4:63835055-63835074 | Msa0341910:exon | 35.0% | |
| ACCATAACAGTTTCACAAAC+CGG | - | chr2_4:63835088-63835107 | Msa0341910:five_prime_UTR | 35.0% | |
| TAAGGAAATCACTTGTCCTT+TGG | - | chr2_4:63835195-63835214 | Msa0341910:five_prime_UTR | 35.0% | |
| !!! | GAGTATTTTTGAGATTGAGG+AGG | - | chr2_4:63835355-63835374 | Msa0341910:five_prime_UTR | 35.0% |
| TCAGAAACTTCTCTTGCAAA+TGG | + | chr2_4:63835382-63835401 | None:intergenic | 35.0% | |
| GTTTGCACTTTGCAAGGAAA+TGG | + | chr2_4:63833164-63833183 | None:intergenic | 40.0% | |
| ! | GAGTTTGTTTGCACTTTGCA+AGG | + | chr2_4:63833170-63833189 | None:intergenic | 40.0% |
| CCATCATGTGAATCTCTTGA+AGG | + | chr2_4:63833578-63833597 | None:intergenic | 40.0% | |
| CCTTCAAGAGATTCACATGA+TGG | - | chr2_4:63833575-63833594 | Msa0341910:intron | 40.0% | |
| ! | TGATATTGGAGTAGCTTGCA+TGG | + | chr2_4:63833615-63833634 | None:intergenic | 40.0% |
| ! | TCTCCTTCACATCCTTCTTT+TGG | - | chr2_4:63833650-63833669 | Msa0341910:intron | 40.0% |
| !!! | GGGATTTTTAGGTCATCTTC+AGG | - | chr2_4:63833710-63833729 | Msa0341910:CDS | 40.0% |
| GAAGGAAAGGTAGTAGAAAG+AGG | - | chr2_4:63833732-63833751 | Msa0341910:CDS | 40.0% | |
| GAAAGAGGAATGATAAAGGG+TGG | - | chr2_4:63833747-63833766 | Msa0341910:CDS | 40.0% | |
| TCAAGTATAACACCACACTC+AGG | + | chr2_4:63833773-63833792 | None:intergenic | 40.0% | |
| AGGTTCTTATCGTGAATTCG+AGG | - | chr2_4:63833796-63833815 | Msa0341910:CDS | 40.0% | |
| ACAGTAACTTCTGCCTTGAA+AGG | + | chr2_4:63833920-63833939 | None:intergenic | 40.0% | |
| !! | GTGGACCATCTTTTTGAAGA+AGG | - | chr2_4:63834241-63834260 | Msa0341910:intron | 40.0% |
| ATAGTAGGTGCAAGTGCAAA+TGG | + | chr2_4:63834317-63834336 | None:intergenic | 40.0% | |
| GGATGCCAAAAAATGTAGCA+GGG | + | chr2_4:63834781-63834800 | None:intergenic | 40.0% | |
| TGGATGCCAAAAAATGTAGC+AGG | + | chr2_4:63834782-63834801 | None:intergenic | 40.0% | |
| TCAGATATCAGTATCGCAAC+CGG | - | chr2_4:63834833-63834852 | Msa0341910:CDS | 40.0% | |
| ACAATACGGTGACTCTTTCT+TGG | + | chr2_4:63835019-63835038 | None:intergenic | 40.0% | |
| CCGTATTGTATCAGTGAACT+TGG | - | chr2_4:63835030-63835049 | Msa0341910:CDS | 40.0% | |
| ! | TCCGGTTTGTGAAACTGTTA+TGG | + | chr2_4:63835092-63835111 | None:intergenic | 40.0% |
| !! | AAGTGCTACTTCAACACTTC+CGG | + | chr2_4:63835110-63835129 | None:intergenic | 40.0% |
| ! | CCAGCAGAATTGATAACCAA+AGG | + | chr2_4:63835214-63835233 | None:intergenic | 40.0% |
| CCTTTGGTTATCAATTCTGC+TGG | - | chr2_4:63835211-63835230 | Msa0341910:five_prime_UTR | 40.0% | |
| TTTCAGCAGCCAGTGATGTT+TGG | - | chr2_4:63833036-63833055 | Msa0341910:three_prime_UTR | 45.0% | |
| !! | GGAGATCTTGATGGTGAAGA+TGG | + | chr2_4:63833527-63833546 | None:intergenic | 45.0% |
| ! | GGTCTCTTTGGAGATCTTGA+TGG | + | chr2_4:63833536-63833555 | None:intergenic | 45.0% |
| CAGAAGAGTTTCTCGAGTGA+CGG | + | chr2_4:63833676-63833695 | None:intergenic | 45.0% | |
| CGAGAAACTCTTCTGCTAGT+CGG | - | chr2_4:63833681-63833700 | Msa0341910:intron | 45.0% | |
| !! | CTCTTCTGCTAGTCGGTTTT+CGG | - | chr2_4:63833688-63833707 | Msa0341910:intron | 45.0% |
| !! | TCTTCTGCTAGTCGGTTTTC+GGG | - | chr2_4:63833689-63833708 | Msa0341910:intron | 45.0% |
| !! | GTCGGTTTTCGGGGATTTTT+AGG | - | chr2_4:63833699-63833718 | Msa0341910:CDS | 45.0% |
| AGGTCATCTTCAGGAAGGAA+AGG | - | chr2_4:63833719-63833738 | Msa0341910:CDS | 45.0% | |
| CAAGGCAGAAGTTACTGTCA+AGG | - | chr2_4:63833922-63833941 | Msa0341910:intron | 45.0% | |
| ! | TTTTTGTTGGGACTCCTGTG+TGG | + | chr2_4:63834718-63834737 | None:intergenic | 45.0% |
| CAAGATTGATCGGTGAAGTG+TGG | + | chr2_4:63834808-63834827 | None:intergenic | 45.0% | |
| AAGATTCCCCTTTATGGAGC+TGG | - | chr2_4:63835061-63835080 | Msa0341910:exon | 45.0% | |
| GTTGATCCAGCTCCATAAAG+GGG | + | chr2_4:63835070-63835089 | None:intergenic | 45.0% | |
| GGTTGATCCAGCTCCATAAA+GGG | + | chr2_4:63835071-63835090 | None:intergenic | 45.0% | |
| TGGTTGATCCAGCTCCATAA+AGG | + | chr2_4:63835072-63835091 | None:intergenic | 45.0% | |
| ! | AAGTTTGAAATCCGATCGCG+AGG | - | chr2_4:63835136-63835155 | Msa0341910:five_prime_UTR | 45.0% |
| ! | GGTAAGGACAAGGCATCATA+AGG | - | chr2_4:63835177-63835196 | Msa0341910:five_prime_UTR | 45.0% |
| ! | TCACTGGCTGCTGAAAGTGT+CGG | + | chr2_4:63833032-63833051 | None:intergenic | 50.0% |
| GGATGTGAAGGAGACTCCAT+AGG | + | chr2_4:63833644-63833663 | None:intergenic | 50.0% | |
| CGGCCAAAAGAAGGATGTGA+AGG | + | chr2_4:63833656-63833675 | None:intergenic | 50.0% | |
| !! | CTTCTGCTAGTCGGTTTTCG+GGG | - | chr2_4:63833690-63833709 | Msa0341910:CDS | 50.0% |
| ! | TGATCATTTGGGGTGCTAGC+AGG | - | chr2_4:63833894-63833913 | Msa0341910:intron | 50.0% |
| TGATCGGTGAAGTGTGGACA+TGG | + | chr2_4:63834802-63834821 | None:intergenic | 50.0% | |
| TATCAGTATCGCAACCGGTG+AGG | - | chr2_4:63834838-63834857 | Msa0341910:CDS | 50.0% | |
| ! | ATGTGGTGGGGAAACTGGTA+AGG | - | chr2_4:63835161-63835180 | Msa0341910:five_prime_UTR | 50.0% |
| TGGGGAAACTGGTAAGGACA+AGG | - | chr2_4:63835167-63835186 | Msa0341910:five_prime_UTR | 50.0% | |
| CTCGAGTGACGGCCAAAAGA+AGG | + | chr2_4:63833665-63833684 | None:intergenic | 55.0% | |
| GATAAAGGGTGGCCTGAGTG+TGG | - | chr2_4:63833758-63833777 | Msa0341910:CDS | 55.0% | |
| AATCCGATCGCGAGGTGATG+TGG | - | chr2_4:63835144-63835163 | Msa0341910:five_prime_UTR | 55.0% | |
| ! | AGGTGATGTGGTGGGGAAAC+TGG | - | chr2_4:63835156-63835175 | Msa0341910:five_prime_UTR | 55.0% |
| GGGTGCTAGCAGGCCTTTCA+AGG | - | chr2_4:63833904-63833923 | Msa0341910:intron | 60.0% | |
| ! | TGGGAGGGTACAGACCACAC+AGG | - | chr2_4:63834701-63834720 | Msa0341910:CDS | 60.0% |
| CCACCACATCACCTCGCGAT+CGG | + | chr2_4:63835150-63835169 | None:intergenic | 60.0% | |
| CGATCGCGAGGTGATGTGGT+GGG | - | chr2_4:63835148-63835167 | Msa0341910:five_prime_UTR | 60.0% | |
| GATCGCGAGGTGATGTGGTG+GGG | - | chr2_4:63835149-63835168 | Msa0341910:five_prime_UTR | 60.0% | |
| CCGATCGCGAGGTGATGTGG+TGG | - | chr2_4:63835147-63835166 | Msa0341910:five_prime_UTR | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 63833019 | 63835530 | 63833019 | ID=Msa0341910;Name=Msa0341910 |
| chr2_4 | mRNA | 63833019 | 63835530 | 63833019 | ID=Msa0341910-mRNA-1;Parent=Msa0341910;Name=Msa0341910-mRNA-1;_AED=0.04;_eAED=0.04;_QI=463|1|1|1|1|1|3|251|309 |
| chr2_4 | exon | 63833019 | 63833551 | 63833019 | ID=Msa0341910-mRNA-1:exon:20457;Parent=Msa0341910-mRNA-1 |
| chr2_4 | exon | 63833690 | 63833875 | 63833690 | ID=Msa0341910-mRNA-1:exon:20456;Parent=Msa0341910-mRNA-1 |
| chr2_4 | exon | 63834606 | 63835530 | 63834606 | ID=Msa0341910-mRNA-1:exon:20455;Parent=Msa0341910-mRNA-1 |
| chr2_4 | five_prime_UTR | 63835068 | 63835530 | 63835068 | ID=Msa0341910-mRNA-1:five_prime_utr;Parent=Msa0341910-mRNA-1 |
| chr2_4 | CDS | 63834606 | 63835067 | 63834606 | ID=Msa0341910-mRNA-1:cds;Parent=Msa0341910-mRNA-1 |
| chr2_4 | CDS | 63833690 | 63833875 | 63833690 | ID=Msa0341910-mRNA-1:cds;Parent=Msa0341910-mRNA-1 |
| chr2_4 | CDS | 63833270 | 63833551 | 63833270 | ID=Msa0341910-mRNA-1:cds;Parent=Msa0341910-mRNA-1 |
| chr2_4 | three_prime_UTR | 63833019 | 63833269 | 63833019 | ID=Msa0341910-mRNA-1:three_prime_utr;Parent=Msa0341910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
