AlfalfaGEDB | Msa1269230

M. sativa cultivar zhongmu-4 / Msa1269230

KEGG: K12854
GO: GO:0000245 GO:0000354 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009506 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0046540 GO:0055044 GO:0065003 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904
PFAM: PF02889.19

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1269230	RHN79081.1	89.697	165	9	1	1	157	410	574	6.70e-100	309

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1269230	sp\|O48534\|DEXHD_ARATH	69.512	164	42	1	1	156	1976	2139	2.05e-74	244

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1269230	A0A396JQU6	89.697	165	9	1	1	157	410	574	3.20e-100	309

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa1269230	0.813335	2.697160e-51	-8.615850e-47
Msa0014710	Msa1269230	0.801986	6.951261e-49	-8.615850e-47
Msa0015390	Msa1269230	0.829583	4.746506e-55	-8.615850e-47
Msa0017510	Msa1269230	0.855887	4.664317e-62	-8.615850e-47
Msa0028640	Msa1269230	0.810078	1.378350e-50	-8.615850e-47
Msa0050390	Msa1269230	0.801221	9.974698e-49	-8.615850e-47
Msa0054480	Msa1269230	0.803134	4.029048e-49	-8.615850e-47
Msa0062600	Msa1269230	0.803179	3.943720e-49	-8.615850e-47
Msa0078900	Msa1269230	0.811834	5.741713e-51	-8.615850e-47
Msa0086760	Msa1269230	0.821304	4.336107e-53	-8.615850e-47
Msa0107120	Msa1269230	0.800916	1.151361e-48	-8.615850e-47
Msa0107180	Msa1269230	0.811913	5.519714e-51	-8.615850e-47
Msa0114060	Msa1269230	0.825499	4.533147e-54	-8.615850e-47
Msa0610430	Msa1269230	0.816699	4.832728e-52	-8.615850e-47
Msa0613440	Msa1269230	0.808078	3.696289e-50	-8.615850e-47
Msa0616160	Msa1269230	0.805334	1.403675e-49	-8.615850e-47
Msa0621490	Msa1269230	0.806556	7.766023e-50	-8.615850e-47
Msa0625720	Msa1269230	0.823438	1.384737e-53	-8.615850e-47
Msa0627450	Msa1269230	0.802382	5.761836e-49	-8.615850e-47
Msa0648880	Msa1269230	0.801983	6.959644e-49	-8.615850e-47
Msa0657970	Msa1269230	0.805564	1.256062e-49	-8.615850e-47
Msa0661190	Msa1269230	-0.813114	3.015023e-51	-8.615850e-47
Msa0661650	Msa1269230	0.821708	3.498162e-53	-8.615850e-47
Msa0663380	Msa1269230	0.810723	1.000318e-50	-8.615850e-47
Msa0663390	Msa1269230	0.811682	6.197269e-51	-8.615850e-47
Msa0669320	Msa1269230	0.804671	1.931614e-49	-8.615850e-47
Msa0681270	Msa1269230	0.806984	6.306944e-50	-8.615850e-47
Msa0688270	Msa1269230	0.800167	1.636428e-48	-8.615850e-47
Msa0688480	Msa1269230	0.807327	5.334926e-50	-8.615850e-47
Msa0691380	Msa1269230	0.806246	9.028398e-50	-8.615850e-47
Msa0731940	Msa1269230	0.828751	7.553216e-55	-8.615850e-47
Msa0735170	Msa1269230	0.801895	7.255366e-49	-8.615850e-47
Msa0743720	Msa1269230	0.832744	7.935230e-56	-8.615850e-47
Msa0762970	Msa1269230	0.806479	8.063393e-50	-8.615850e-47
Msa0764570	Msa1269230	0.803221	3.865194e-49	-8.615850e-47
Msa0766630	Msa1269230	0.801389	9.215632e-49	-8.615850e-47
Msa0767760	Msa1269230	0.800822	1.203468e-48	-8.615850e-47
Msa0769920	Msa1269230	0.827676	1.371257e-54	-8.615850e-47
Msa0777850	Msa1269230	0.810558	1.086146e-50	-8.615850e-47
Msa0793800	Msa1269230	0.807912	4.007909e-50	-8.615850e-47
Msa0796700	Msa1269230	0.809626	1.724270e-50	-8.615850e-47
Msa0806660	Msa1269230	0.809736	1.632751e-50	-8.615850e-47
Msa0822870	Msa1269230	0.838256	3.203468e-57	-8.615850e-47
Msa0832340	Msa1269230	0.807083	6.009225e-50	-8.615850e-47
Msa0127660	Msa1269230	0.800731	1.255720e-48	-8.615850e-47
Msa0131770	Msa1269230	0.822017	2.965695e-53	-8.615850e-47
Msa0135380	Msa1269230	0.807842	4.147555e-50	-8.615850e-47
Msa0136070	Msa1269230	0.809961	1.460429e-50	-8.615850e-47
Msa0157290	Msa1269230	0.855219	7.305154e-62	-8.615850e-47
Msa0160550	Msa1269230	0.801211	1.001932e-48	-8.615850e-47
Msa0165210	Msa1269230	0.801117	1.047469e-48	-8.615850e-47
Msa0165220	Msa1269230	0.809127	2.206256e-50	-8.615850e-47
Msa0171540	Msa1269230	0.810121	1.348905e-50	-8.615850e-47
Msa0180500	Msa1269230	0.809201	2.126650e-50	-8.615850e-47
Msa0211150	Msa1269230	0.816978	4.183790e-52	-8.615850e-47
Msa0215320	Msa1269230	-0.804691	1.913075e-49	-8.615850e-47
Msa0215680	Msa1269230	0.813620	2.334737e-51	-8.615850e-47
Msa0220580	Msa1269230	0.800493	1.404821e-48	-8.615850e-47
Msa1226660	Msa1269230	0.803392	3.563207e-49	-8.615850e-47
Msa1242220	Msa1269230	0.808636	2.809519e-50	-8.615850e-47
Msa1242240	Msa1269230	0.805472	1.313123e-49	-8.615850e-47
Msa1242670	Msa1269230	0.817833	2.686652e-52	-8.615850e-47
Msa1244250	Msa1269230	0.835495	1.623340e-56	-8.615850e-47
Msa1252140	Msa1269230	0.820216	7.713074e-53	-8.615850e-47
Msa1269230	Msa1277660	0.836846	7.366109e-57	-8.615850e-47
Msa1269230	Msa1282580	-0.803340	3.652320e-49	-8.615850e-47
Msa1269230	Msa1283840	0.816610	5.060142e-52	-8.615850e-47
Msa1269230	Msa1283860	0.806186	9.297077e-50	-8.615850e-47
Msa1269230	Msa1284260	0.815573	8.628027e-52	-8.615850e-47
Msa1269230	Msa1284680	0.819658	1.034475e-52	-8.615850e-47
Msa1269230	Msa1286800	0.801630	8.224524e-49	-8.615850e-47
Msa1269230	Msa1287460	0.813145	2.968740e-51	-8.615850e-47
Msa1269230	Msa1322890	0.807159	5.790630e-50	-8.615850e-47
Msa1269230	Msa1327050	0.808159	3.551841e-50	-8.615850e-47
Msa1269230	Msa1327680	0.806473	8.087429e-50	-8.615850e-47
Msa1269230	Msa1336670	0.823681	1.214955e-53	-8.615850e-47
Msa1269230	Msa1340470	0.806482	8.050020e-50	-8.615850e-47
Msa1269230	Msa1342840	0.804599	1.999135e-49	-8.615850e-47
Msa1269230	Msa1348890	0.800847	1.189169e-48	-8.615850e-47
Msa1269230	Msa1353470	0.813922	2.002501e-51	-8.615850e-47
Msa1269230	Msa1359040	0.824783	6.693896e-54	-8.615850e-47
Msa1269230	Msa1372760	0.810406	1.170972e-50	-8.615850e-47
Msa1269230	Msa1373030	0.800542	1.372325e-48	-8.615850e-47
Msa1269230	Msa1379500	0.824866	6.399154e-54	-8.615850e-47
Msa1269230	Msa1392260	0.804099	2.540901e-49	-8.615850e-47
Msa1269230	Msa1402730	0.811349	7.319699e-51	-8.615850e-47
Msa1269230	Msa1409870	0.817325	3.497018e-52	-8.615850e-47
Msa1269230	Msa1410680	0.801991	6.932215e-49	-8.615850e-47
Msa1269230	Msa1411020	0.804762	1.848616e-49	-8.615850e-47
Msa1269230	Msa1429150	0.822710	2.048110e-53	-8.615850e-47
Msa1269230	Msa1434350	0.802567	5.277854e-49	-8.615850e-47
Msa1269230	Msa1439320	0.803243	3.826474e-49	-8.615850e-47
Msa1269230	Msa1452240	0.815792	7.710403e-52	-8.615850e-47
Msa1269230	Msa1464940	0.800776	1.229798e-48	-8.615850e-47
Msa1269230	Msa1466540	0.827495	1.515680e-54	-8.615850e-47
Msa0367870	Msa1269230	0.800159	1.642895e-48	-8.615850e-47
Msa0390400	Msa1269230	-0.801047	1.082550e-48	-8.615850e-47
Msa0395790	Msa1269230	0.823569	1.290425e-53	-8.615850e-47
Msa0397750	Msa1269230	0.836835	7.411414e-57	-8.615850e-47
Msa0398710	Msa1269230	0.806086	9.759663e-50	-8.615850e-47
Msa0410650	Msa1269230	0.810653	1.035654e-50	-8.615850e-47
Msa0440400	Msa1269230	0.802794	4.738068e-49	-8.615850e-47
Msa0440750	Msa1269230	0.827119	1.865174e-54	-8.615850e-47
Msa0443260	Msa1269230	0.806052	9.917399e-50	-8.615850e-47
Msa0464680	Msa1269230	0.816643	4.974789e-52	-8.615850e-47
Msa0481530	Msa1269230	0.801336	9.447008e-49	-8.615850e-47
Msa0993290	Msa1269230	0.823747	1.172877e-53	-8.615850e-47
Msa0993600	Msa1269230	0.821393	4.136333e-53	-8.615850e-47
Msa0998690	Msa1269230	0.809987	1.442047e-50	-8.615850e-47
Msa1003620	Msa1269230	0.809043	2.298957e-50	-8.615850e-47
Msa1014540	Msa1269230	0.857830	1.249101e-62	-8.615850e-47
Msa1030970	Msa1269230	0.804435	2.163220e-49	-8.615850e-47
Msa1052830	Msa1269230	0.801088	1.061941e-48	-8.615850e-47
Msa1075220	Msa1269230	-0.820074	8.309406e-53	-8.615850e-47
Msa1075540	Msa1269230	0.819489	1.130803e-52	-8.615850e-47
Msa1075650	Msa1269230	0.806472	8.091420e-50	-8.615850e-47
Msa1082500	Msa1269230	0.828155	1.052269e-54	-8.615850e-47
Msa1085200	Msa1269230	0.817802	2.729301e-52	-8.615850e-47
Msa1100050	Msa1269230	0.800984	1.115322e-48	-8.615850e-47
Msa1103980	Msa1269230	0.813830	2.098125e-51	-8.615850e-47
Msa1104090	Msa1269230	0.806126	9.571361e-50	-8.615850e-47
Msa1104370	Msa1269230	0.803248	3.816759e-49	-8.615850e-47
Msa0243100	Msa1269230	0.812977	3.230506e-51	-8.615850e-47
Msa0244860	Msa1269230	0.806557	7.764432e-50	-8.615850e-47
Msa0257460	Msa1269230	0.812493	4.124129e-51	-8.615850e-47
Msa0261340	Msa1269230	0.817252	3.630672e-52	-8.615850e-47
Msa0272690	Msa1269230	0.808590	2.873887e-50	-8.615850e-47
Msa0280090	Msa1269230	0.802279	6.049461e-49	-8.615850e-47
Msa0283450	Msa1269230	0.816329	5.849534e-52	-8.615850e-47
Msa0283520	Msa1269230	0.800055	1.724112e-48	-8.615850e-47
Msa0287650	Msa1269230	0.810943	8.963698e-51	-8.615850e-47
Msa0291540	Msa1269230	0.802322	5.927399e-49	-8.615850e-47
Msa0332000	Msa1269230	0.804678	1.925391e-49	-8.615850e-47
Msa0340450	Msa1269230	0.823503	1.337455e-53	-8.615850e-47
Msa0345970	Msa1269230	0.820240	7.616809e-53	-8.615850e-47
Msa1108160	Msa1269230	0.801260	9.794225e-49	-8.615850e-47
Msa1113170	Msa1269230	0.839023	2.029993e-57	-8.615850e-47
Msa1116130	Msa1269230	-0.801945	7.086875e-49	-8.615850e-47
Msa1121490	Msa1269230	0.817080	3.969244e-52	-8.615850e-47
Msa1131220	Msa1269230	0.815228	1.029299e-51	-8.615850e-47
Msa1137570	Msa1269230	0.801940	7.102645e-49	-8.615850e-47
Msa1154280	Msa1269230	0.805159	1.527404e-49	-8.615850e-47
Msa1162550	Msa1269230	-0.813381	2.634735e-51	-8.615850e-47
Msa1209460	Msa1269230	0.806678	7.319122e-50	-8.615850e-47
Msa0507680	Msa1269230	0.813200	2.887084e-51	-8.615850e-47
Msa0512900	Msa1269230	0.805755	1.145467e-49	-8.615850e-47
Msa0519000	Msa1269230	0.803465	3.441685e-49	-8.615850e-47
Msa0529040	Msa1269230	0.806335	8.647591e-50	-8.615850e-47
Msa0529890	Msa1269230	0.825687	4.092300e-54	-8.615850e-47
Msa0529930	Msa1269230	0.808912	2.453227e-50	-8.615850e-47
Msa0530940	Msa1269230	0.804283	2.327210e-49	-8.615850e-47
Msa0532350	Msa1269230	0.810536	1.097663e-50	-8.615850e-47
Msa0548170	Msa1269230	0.806350	8.582905e-50	-8.615850e-47
Msa0585300	Msa1269230	0.823596	1.271863e-53	-8.615850e-47
Msa0585560	Msa1269230	0.810800	9.627655e-51	-8.615850e-47
Msa0593650	Msa1269230	0.819917	9.029504e-53	-8.615850e-47
Msa0601750	Msa1269230	0.818487	1.910906e-52	-8.615850e-47
Msa0602240	Msa1269230	0.801684	8.016413e-49	-8.615850e-47
Msa0602390	Msa1269230	0.805284	1.438059e-49	-8.615850e-47
Msa0844490	Msa1269230	0.806190	9.277815e-50	-8.615850e-47
Msa0857640	Msa1269230	0.806457	8.149948e-50	-8.615850e-47
Msa0861870	Msa1269230	0.800683	1.284675e-48	-8.615850e-47
Msa0871270	Msa1269230	0.812310	4.521569e-51	-8.615850e-47
Msa0875290	Msa1269230	0.813500	2.480972e-51	-8.615850e-47
Msa0880140	Msa1269230	0.851605	7.957981e-61	-8.615850e-47
Msa0897330	Msa1269230	0.849202	3.761087e-60	-8.615850e-47
Msa0935670	Msa1269230	0.800093	1.694009e-48	-8.615850e-47
Msa0938220	Msa1269230	0.819701	1.011642e-52	-8.615850e-47
Msa0944190	Msa1269230	0.800291	1.543742e-48	-8.615850e-47
Msa0947660	Msa1269230	0.837927	3.893780e-57	-8.615850e-47
Msa0959360	Msa1269230	0.804125	2.510115e-49	-8.615850e-47
Msa0966750	Msa1269230	0.803119	4.057817e-49	-8.615850e-47
Msa0969450	Msa1269230	0.806653	7.411121e-50	-8.615850e-47
Msa0969520	Msa1269230	0.847253	1.299522e-59	-8.615850e-47
Msa0969810	Msa1269230	0.804104	2.535778e-49	-8.615850e-47
Msa0974070	Msa1269230	0.819884	9.185856e-53	-8.615850e-47
Msa0974080	Msa1269230	0.806430	8.257626e-50	-8.615850e-47
Msa0976670	Msa1269230	0.822542	2.240160e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1269230	MtrunA17_Chr1g0173011	89.697	165	9	1	1	157	410	574	6.16e-104	309
Msa1269230	MtrunA17_Chr4g0003841	89.091	165	10	1	1	157	1948	2112	8.22e-96	302
Msa1269230	MtrunA17_Chr2g0312441	80.606	165	24	1	1	157	1994	2158	5.72e-87	277
Msa1269230	MtrunA17_Chr2g0292871	34.545	165	92	8	1	156	778	935	1.26e-15	73.2
Msa1269230	MtrunA17_Chr2g0312351	33.065	124	70	3	1	118	999	1115	1.43e-14	70.1
Msa1269230	MtrunA17_Chr3g0086711	32.515	163	87	7	1	150	1906	2058	6.42e-14	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1269230	AT2G42270.1	69.512	164	42	1	1	156	1976	2139	2.08e-75	244
Msa1269230	AT1G20960.1	68.293	164	44	1	1	156	1975	2138	3.48e-74	241
Msa1269230	AT1G20960.2	68.293	164	44	1	1	156	1975	2138	3.48e-74	241
Msa1269230	AT5G61140.2	30.247	162	81	6	1	145	1976	2122	7.25e-14	68.6
Msa1269230	AT5G61140.1	30.247	162	81	6	1	145	1965	2111	7.32e-14	68.6

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAACCATCTTTGATTTAT+TGG	0.149036	8_3:-2452859	Msa1269230:CDS
CATGCCAAATTCATACTTAT+TGG	0.152145	8_3:-2452802	Msa1269230:CDS
CATCCCTCCTCCTTAGCTTT+TGG	0.188606	8_3:+2452618	None:intergenic
TCCTCATTTCACAAAGGATT+TGG	0.292168	8_3:-2452916	Msa1269230:CDS
TGCAAGTAACGAGTTGGTTT+TGG	0.306839	8_3:+2452575	None:intergenic
ATGTCCAATAAGTATGAATT+TGG	0.325930	8_3:+2452798	None:intergenic
ATGGTTTCTATACTCTTCCC+TGG	0.420890	8_3:+2452873	None:intergenic
TCATACGACAAATCAACATT+AGG	0.425562	8_3:+2452753	None:intergenic
CATCTTTGATTTATTGGAGA+TGG	0.435428	8_3:-2452853	Msa1269230:CDS
TTTGCTGCACCTGCTGATGC+TGG	0.442977	8_3:-2452507	Msa1269230:CDS
CATCTCCAATAAATCAAAGA+TGG	0.462471	8_3:+2452854	None:intergenic
GGCTATTAATTTCCGCTGCA+AGG	0.473607	8_3:+2452539	None:intergenic
TGGTTTCTATACTCTTCCCT+GGG	0.488358	8_3:+2452874	None:intergenic
AGGTGCAGCAAACTGCAGTT+TGG	0.488681	8_3:+2452518	None:intergenic
GCCAAATCCTTTGTGAAATG+AGG	0.495506	8_3:+2452915	None:intergenic
GGATACCTAGGAGCATCAAC+AGG	0.495924	8_3:+2452639	None:intergenic
GATTTCTTTCCAGCATCAGC+AGG	0.500461	8_3:+2452498	None:intergenic
CTCGTTACTTGCAATTAAGC+GGG	0.507205	8_3:-2452565	Msa1269230:CDS
AAATGGTGACACAAGGATTG+TGG	0.517959	8_3:-2452965	Msa1269230:CDS
AATGGTGACACAAGGATTGT+GGG	0.523565	8_3:-2452964	Msa1269230:CDS
CTTAATTGCAAGTAACGAGT+TGG	0.531343	8_3:+2452569	None:intergenic
ATTTCACAAAGGATTTGGCT+AGG	0.535343	8_3:-2452911	Msa1269230:CDS
TTGTTATATCCTCACCAGTC+CGG	0.537433	8_3:+2452709	None:intergenic
TATAACAATATGTGTCACTC+TGG	0.546972	8_3:-2452694	Msa1269230:CDS
ATCGATGGTAAGACAGAAGT+AGG	0.566913	8_3:-2452663	Msa1269230:CDS
TAGGACCTGTTGATGCTCCT+AGG	0.567274	8_3:-2452644	Msa1269230:CDS
GTATCCAAAAGCTAAGGAGG+AGG	0.574559	8_3:-2452622	Msa1269230:CDS
TCCTAGGTATCCAAAAGCTA+AGG	0.587586	8_3:-2452628	Msa1269230:CDS
AGGAAATGTGAGGAGAACCC+AGG	0.594896	8_3:-2452891	Msa1269230:CDS
ACTCGTTACTTGCAATTAAG+CGG	0.599217	8_3:-2452566	Msa1269230:CDS
AATATGTGTCACTCTGGAGA+GGG	0.599575	8_3:-2452688	Msa1269230:CDS
CACAATGACAGTGTCCGGAC+TGG	0.599754	8_3:-2452723	Msa1269230:intron
TAGGTATCCAAAAGCTAAGG+AGG	0.605710	8_3:-2452625	Msa1269230:CDS
ACAACTTCCTCATTTCACAA+AGG	0.617834	8_3:-2452922	Msa1269230:CDS
CAATATGTGTCACTCTGGAG+AGG	0.618178	8_3:-2452689	Msa1269230:CDS
TATCCAAAAGCTAAGGAGGA+GGG	0.622215	8_3:-2452621	Msa1269230:CDS
CAAAAGCTAAGGAGGAGGGA+TGG	0.631038	8_3:-2452617	Msa1269230:CDS
AAGCTAAGGAGGAGGGATGG+TGG	0.635031	8_3:-2452614	Msa1269230:CDS
ATGGTGACACAAGGATTGTG+GGG	0.640000	8_3:-2452963	Msa1269230:CDS
GGAAATGTGAGGAGAACCCA+GGG	0.653582	8_3:-2452890	Msa1269230:CDS
GTTAGTCAAATGGTGACACA+AGG	0.665050	8_3:-2452972	None:intergenic
TGACAGTGTCCGGACTGGTG+AGG	0.670526	8_3:-2452718	Msa1269230:intron
TGGAGATGGAAGATGTTGCG+AGG	0.674311	8_3:-2452839	Msa1269230:CDS
GGATTTGGCTAGGAAATGTG+AGG	0.680853	8_3:-2452901	Msa1269230:CDS
TTAAGCGGGTTTCCTTGCAG+CGG	0.683267	8_3:-2452551	Msa1269230:CDS
ACTCTGGAGAGGGATATCGA+TGG	0.739537	8_3:-2452678	Msa1269230:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAACCATCTTTGATTTAT+TGG	-	chr8_3:2452595-2452614	Msa1269230:CDS	25.0%
!	ATGTCCAATAAGTATGAATT+TGG	+	chr8_3:2452659-2452678	None:intergenic	25.0%
	CATCTCCAATAAATCAAAGA+TGG	+	chr8_3:2452603-2452622	None:intergenic	30.0%
!	CATCTTTGATTTATTGGAGA+TGG	-	chr8_3:2452601-2452620	Msa1269230:CDS	30.0%
	CATGCCAAATTCATACTTAT+TGG	-	chr8_3:2452652-2452671	Msa1269230:CDS	30.0%
	TCATACGACAAATCAACATT+AGG	+	chr8_3:2452704-2452723	None:intergenic	30.0%
!	TATAACAATATGTGTCACTC+TGG	-	chr8_3:2452760-2452779	Msa1269230:CDS	30.0%
	ACAACTTCCTCATTTCACAA+AGG	-	chr8_3:2452532-2452551	Msa1269230:CDS	35.0%
	TCCTCATTTCACAAAGGATT+TGG	-	chr8_3:2452538-2452557	Msa1269230:CDS	35.0%
	ATTTCACAAAGGATTTGGCT+AGG	-	chr8_3:2452543-2452562	Msa1269230:CDS	35.0%
	CTTAATTGCAAGTAACGAGT+TGG	+	chr8_3:2452888-2452907	None:intergenic	35.0%
	ACTCGTTACTTGCAATTAAG+CGG	-	chr8_3:2452888-2452907	Msa1269230:CDS	35.0%
	GCCAAATCCTTTGTGAAATG+AGG	+	chr8_3:2452542-2452561	None:intergenic	40.0%
	TGGTTTCTATACTCTTCCCT+GGG	+	chr8_3:2452583-2452602	None:intergenic	40.0%
	ATGGTTTCTATACTCTTCCC+TGG	+	chr8_3:2452584-2452603	None:intergenic	40.0%
	TTGTTATATCCTCACCAGTC+CGG	+	chr8_3:2452748-2452767	None:intergenic	40.0%
!	AATATGTGTCACTCTGGAGA+GGG	-	chr8_3:2452766-2452785	Msa1269230:CDS	40.0%
	ATCGATGGTAAGACAGAAGT+AGG	-	chr8_3:2452791-2452810	Msa1269230:CDS	40.0%
	TCCTAGGTATCCAAAAGCTA+AGG	-	chr8_3:2452826-2452845	Msa1269230:CDS	40.0%
!!	TCCTTAGCTTTTGGATACCT+AGG	+	chr8_3:2452830-2452849	None:intergenic	40.0%
	TAGGTATCCAAAAGCTAAGG+AGG	-	chr8_3:2452829-2452848	Msa1269230:CDS	40.0%
	TATCCAAAAGCTAAGGAGGA+GGG	-	chr8_3:2452833-2452852	Msa1269230:CDS	40.0%
!!!	TGCAAGTAACGAGTTGGTTT+TGG	+	chr8_3:2452882-2452901	None:intergenic	40.0%
	CTCGTTACTTGCAATTAAGC+GGG	-	chr8_3:2452889-2452908	Msa1269230:CDS	40.0%
	ATGGTGACACAAGGATTGTG+GGG	-	chr8_3:2452491-2452510	Msa1269230:CDS	45.0%
	GGATTTGGCTAGGAAATGTG+AGG	-	chr8_3:2452553-2452572	Msa1269230:CDS	45.0%
	TACTGCACAATGACAGTGTC+CGG	-	chr8_3:2452726-2452745	Msa1269230:intron	45.0%
!	CAATATGTGTCACTCTGGAG+AGG	-	chr8_3:2452765-2452784	Msa1269230:CDS	45.0%
	GTATCCAAAAGCTAAGGAGG+AGG	-	chr8_3:2452832-2452851	Msa1269230:CDS	45.0%
	GGCTATTAATTTCCGCTGCA+AGG	+	chr8_3:2452918-2452937	None:intergenic	45.0%
	GATTTCTTTCCAGCATCAGC+AGG	+	chr8_3:2452959-2452978	None:intergenic	45.0%
	AGGAAATGTGAGGAGAACCC+AGG	-	chr8_3:2452563-2452582	Msa1269230:CDS	50.0%
	GGAAATGTGAGGAGAACCCA+GGG	-	chr8_3:2452564-2452583	Msa1269230:CDS	50.0%
	TGGAGATGGAAGATGTTGCG+AGG	-	chr8_3:2452615-2452634	Msa1269230:CDS	50.0%
!	ACTCTGGAGAGGGATATCGA+TGG	-	chr8_3:2452776-2452795	Msa1269230:CDS	50.0%
	TAGGACCTGTTGATGCTCCT+AGG	-	chr8_3:2452810-2452829	Msa1269230:CDS	50.0%
!!	GGATACCTAGGAGCATCAAC+AGG	+	chr8_3:2452818-2452837	None:intergenic	50.0%
!!	CATCCCTCCTCCTTAGCTTT+TGG	+	chr8_3:2452839-2452858	None:intergenic	50.0%
	CAAAAGCTAAGGAGGAGGGA+TGG	-	chr8_3:2452837-2452856	Msa1269230:CDS	50.0%
!!!	GAGGGATGGTGGCTTGTTTT+TGG	-	chr8_3:2452851-2452870	Msa1269230:CDS	50.0%
!	TTAAGCGGGTTTCCTTGCAG+CGG	-	chr8_3:2452903-2452922	Msa1269230:CDS	50.0%
	AGGTGCAGCAAACTGCAGTT+TGG	+	chr8_3:2452939-2452958	None:intergenic	50.0%
	CACAATGACAGTGTCCGGAC+TGG	-	chr8_3:2452731-2452750	Msa1269230:intron	55.0%
	AAGCTAAGGAGGAGGGATGG+TGG	-	chr8_3:2452840-2452859	Msa1269230:CDS	55.0%
	TTTGCTGCACCTGCTGATGC+TGG	-	chr8_3:2452947-2452966	Msa1269230:CDS	55.0%
!!	TGACAGTGTCCGGACTGGTG+AGG	-	chr8_3:2452736-2452755	Msa1269230:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	2452491	2452985	2452491	ID=Msa1269230;Name=Msa1269230
chr8_3	mRNA	2452491	2452985	2452491	ID=Msa1269230-mRNA-1;Parent=Msa1269230;Name=Msa1269230-mRNA-1;_AED=0.43;_eAED=0.77;_QI=0\|0\|0\|1\|0\|0\|2\|0\|157
chr8_3	exon	2452759	2452985	2452759	ID=Msa1269230-mRNA-1:exon:769;Parent=Msa1269230-mRNA-1
chr8_3	exon	2452491	2452734	2452491	ID=Msa1269230-mRNA-1:exon:768;Parent=Msa1269230-mRNA-1
chr8_3	CDS	2452759	2452985	2452759	ID=Msa1269230-mRNA-1:cds;Parent=Msa1269230-mRNA-1
chr8_3	CDS	2452491	2452734	2452491	ID=Msa1269230-mRNA-1:cds;Parent=Msa1269230-mRNA-1

Gene Sequence

>Msa1269230

ATGGTGACACAAGGATTGTGGGGACGTGACTCTATGCTGCTACAACTTCCTCATTTCACAAAGGATTTGGCTAGGAAATGTGAGGAGAACCCAGGGAAGAGTATAGAAACCATCTTTGATTTATTGGAGATGGAAGATGTTGCGAGGCGTGAATTGTTAAACATGCCAAATTCATACTTATTGGACATTGCTCGCTTTTGTAATCGCTTTCCTAATGTTGATTTGTCTGTCCGGACTGGTGAGGATATAACAATATGTGTCACTCTGGAGAGGGATATCGATGGTAAGACAGAAGTAGGACCTGTTGATGCTCCTAGGTATCCAAAAGCTAAGGAGGAGGGATGGTGGCTTGTTTTTGGTGATGCCAAAACCAACTCGTTACTTGCAATTAAGCGGGTTTCCTTGCAGCGGAAATTAATAGCCAAACTGCAGTTTGCTGCACCTGCTGATGCTGGAAAGAAATCTTACATA

Protein sequence

>Msa1269230

MVTQGLWGRDSMLLQLPHFTKDLARKCEENPGKSIETIFDLLEMEDVARRELLNMPNSYLLDIARFCNRFPNVDLSVRTGEDITICVTLERDIDGKTEVGPVDAPRYPKAKEEGWWLVFGDAKTNSLLAIKRVSLQRKLIAKLQFAAPADAGKKSYI