AlfalfaGEDB | Msa0942200

M. sativa cultivar zhongmu-4 / Msa0942200

KEGG: -
GO: -
PFAM: PF08646.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0942200	AES70473.1	33.520	358	201	6	35	360	166	518	8.64e-64	224

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa0942200	0.807146	5.827633e-50	-8.615850e-47
Msa0006110	Msa0942200	0.813845	2.082185e-51	-8.615850e-47
Msa0011170	Msa0942200	0.833228	6.016423e-56	-8.615850e-47
Msa0011660	Msa0942200	-0.820961	5.199766e-53	-8.615850e-47
Msa0012060	Msa0942200	0.815160	1.065820e-51	-8.615850e-47
Msa0014710	Msa0942200	0.811840	5.724643e-51	-8.615850e-47
Msa0015390	Msa0942200	0.802014	6.857064e-49	-8.615850e-47
Msa0017070	Msa0942200	0.810550	1.090028e-50	-8.615850e-47
Msa0017510	Msa0942200	0.861638	8.915620e-64	-8.615850e-47
Msa0018650	Msa0942200	0.810143	1.334506e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0942200	MtrunA17_Chr5g0421991	38.911	257	140	5	135	386	12	256	1.12e-46	161
Msa0942200	MtrunA17_Chr1g0167871	30.899	356	197	6	34	352	341	684	1.16e-44	164
Msa0942200	MtrunA17_Chr4g0044661	27.072	362	212	8	35	378	144	471	2.39e-34	133
Msa0942200	MtrunA17_Chr5g0393521	34.222	225	139	3	135	352	15	237	3.00e-32	123
Msa0942200	MtrunA17_Chr5g0417421	28.814	295	147	6	31	317	32	271	5.89e-30	117
Msa0942200	MtrunA17_Chr2g0304851	26.749	243	137	3	33	252	139	363	1.65e-22	98.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 78 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAATTACATGTTTCA+GGG	0.489492	6_3:-16670814	None:intergenic
AAAAGGGTTTCCATATTAGA+TGG	0.424805	6_3:+16670999	Msa0942200:CDS
AAAGCTGTTGAATTTAACTA+TGG	0.346330	6_3:+16671422	Msa0942200:CDS
AAATAAATTATACCAGTTGC+AGG	0.415112	6_3:-16670551	None:intergenic
AAGACGACAAATTGGGAAGA+AGG	0.515939	6_3:+16672214	Msa0942200:CDS
AAGAGAATCATATGCAATTT+TGG	0.149359	6_3:-16671640	None:intergenic
AAGGTGGTTACTTCAAGGAG+TGG	0.630951	6_3:+16670945	Msa0942200:CDS
AATTTCTCATGAGAGACACT+AGG	0.669361	6_3:+16670322	Msa0942200:CDS
ACTGAGTATGATCCAGAGAA+CGG	0.653132	6_3:+16671793	Msa0942200:CDS
ACTTGCTGAACTGTACTTTG+TGG	0.551094	6_3:+16670438	Msa0942200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATCTTCTAATGAGTCAAAT+GGG	-	chr6_3:16670034-16670053	None:intergenic	30.0%
AGATCTTCTAATGAGTCAAA+TGG	-	chr6_3:16670035-16670054	None:intergenic	30.0%
AGAAGATCTAAATGATTCCA+AGG	+	chr6_3:16670046-16670065	Msa0942200:CDS	30.0%
TAAATGATTCCAAGGAAACT+TGG	+	chr6_3:16670054-16670073	Msa0942200:CDS	30.0%
TCTGTTGTCTTAGATATCAT+TGG	+	chr6_3:16670243-16670262	Msa0942200:intron	30.0%
AAAAGGGTTTCCATATTAGA+TGG	+	chr6_3:16670999-16671018	Msa0942200:CDS	30.0%
CTTTAGTTGACCATCTAATA+TGG	-	chr6_3:16671012-16671031	None:intergenic	30.0%
GAGTAGGTTCATTATTAACA+TGG	-	chr6_3:16671046-16671065	None:intergenic	30.0%
TTAATAATGAACCTACTCCA+AGG	+	chr6_3:16671048-16671067	Msa0942200:CDS	30.0%
ATGTAGTTAAATGGTTAGTG+AGG	-	chr6_3:16671075-16671094	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	16670014	16672390	16670014	ID=Msa0942200;Name=Msa0942200
chr6_3	mRNA	16670014	16672390	16670014	ID=Msa0942200-mRNA-1;Parent=Msa0942200;Name=Msa0942200-mRNA-1;_AED=0.09;_eAED=0.10;_QI=0\|0.4\|0.36\|1\|0.9\|0.81\|11\|123\|413
chr6_3	exon	16670014	16670113	16670014	ID=Msa0942200-mRNA-1:exon:5065;Parent=Msa0942200-mRNA-1
chr6_3	exon	16670256	16670343	16670256	ID=Msa0942200-mRNA-1:exon:5066;Parent=Msa0942200-mRNA-1
chr6_3	exon	16670433	16670560	16670433	ID=Msa0942200-mRNA-1:exon:5067;Parent=Msa0942200-mRNA-1
chr6_3	exon	16670661	16670824	16670661	ID=Msa0942200-mRNA-1:exon:5068;Parent=Msa0942200-mRNA-1
chr6_3	exon	16670912	16671069	16670912	ID=Msa0942200-mRNA-1:exon:5069;Parent=Msa0942200-mRNA-1
chr6_3	exon	16671153	16671273	16671153	ID=Msa0942200-mRNA-1:exon:5070;Parent=Msa0942200-mRNA-1
chr6_3	exon	16671378	16671551	16671378	ID=Msa0942200-mRNA-1:exon:5071;Parent=Msa0942200-mRNA-1
chr6_3	exon	16671627	16671686	16671627	ID=Msa0942200-mRNA-1:exon:5072;Parent=Msa0942200-mRNA-1
chr6_3	exon	16671769	16671855	16671769	ID=Msa0942200-mRNA-1:exon:5073;Parent=Msa0942200-mRNA-1
chr6_3	exon	16672085	16672245	16672085	ID=Msa0942200-mRNA-1:exon:5074;Parent=Msa0942200-mRNA-1
chr6_3	exon	16672267	16672390	16672267	ID=Msa0942200-mRNA-1:exon:5075;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16670014	16670113	16670014	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16670256	16670343	16670256	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16670433	16670560	16670433	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16670661	16670824	16670661	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16670912	16671069	16670912	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16671153	16671273	16671153	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16671378	16671551	16671378	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16671627	16671686	16671627	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16671769	16671855	16671769	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16672085	16672245	16672085	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	CDS	16672267	16672267	16672267	ID=Msa0942200-mRNA-1:cds;Parent=Msa0942200-mRNA-1
chr6_3	three_prime_UTR	16672268	16672390	16672268	ID=Msa0942200-mRNA-1:three_prime_utr;Parent=Msa0942200-mRNA-1

Gene Sequence

>Msa0942200

GATGAAGACTTGGAACGCCCATTTGACTCATTAGAAGATCTAAATGATTCCAAGGAAACTTGGAAATTTGCTGTCCGAATTGTTAATCTGTGGACTGCTCATATCATTGGAGCATTTCATGAAATGAAGTTTACCCAAACAAATAGTTATAAGAAAGCAAAGATCAAATTTCTCATGAGAGACACTAGTGGGAACTTGCTGAACTGTACTTTGTGGGACAAGTACGCTAGATCCTTTCGCAGTTTGACCAATCAGACAGGGGGACAATACCCTATTGTTTTCATTATTAGGAATGCAGCCATTCAGCCTGCAACTGAAACATATCCGGTATGTGTTACAAATGCATGGAGTGGAACCAGAATCATTACCAATCAAGATTTACAAGAAATCAAAGAATTTAAGAATAAATTCTCTAATGAGGATAAGATCATATTCAGAGCTGAAGTGCTTACACTCCCACAAATCAATGCCCTGAAACATGAAACCTGTTGCGTGACCGTTGCAAAAACTAACAAGGTGGTTACTTCAAGGAGTGGATGGACTTACGATGGATGTTACATGTGCACCAAAAGGGTTTCCATATTAGATGGTCAACTAAAGTGTGAAAATGGCCATGTTAATAATGAACCTACTCCAAGGTATAAGGTGGATATAGAAGTTGTGTATCGAAATGCTAAATCAAGATTTGTGTTCTTTGATGGAGATTGCACCCAACTCTTAGGAATCTCCGCATCAGACCTTAGGAAAAAAATGATTGCTGCTGGTCAAAAAGATAAGAGAGCTTATCCATACCTGTTCGAAAAAAAGCTGTTGAATTTAACTATGGTATTCAGGGTGAAATGGAGTCCAACATTCAAAACAAGTTCTGTAATGAGGTTCTCGACTGACGAGACTTTCCTTAGCTCAGTTGAGGACAAACTGCTGAAAGAAGAGACAACTGGAGTACCCAAAATTGCATATGATTCTCTTCAGCAATCACAGAATGATCATGTGCAATCAGTCTACCCCTCTGCTACAACTGAGTATGATCCAGAGAACGGTTCGAATACAACTCCTGCAAAAAGGTTGTCTTCAAAAGAGGAGTTGACATCTGATGTTGTGGAGGAAACTCCTACCAGTAAACCCGGAAGAAAAAGATTTGCTGACAATTTTCAACCATGTGCCGTGAATGATTTTTGTGGGACTGAGACATCTTGCACTAAGAAGAGAAAGACGACAAATTGGGAAGAAGGAACCAAATAA

Protein sequence

>Msa0942200

DEDLERPFDSLEDLNDSKETWKFAVRIVNLWTAHIIGAFHEMKFTQTNSYKKAKIKFLMRDTSGNLLNCTLWDKYARSFRSLTNQTGGQYPIVFIIRNAAIQPATETYPVCVTNAWSGTRIITNQDLQEIKEFKNKFSNEDKIIFRAEVLTLPQINALKHETCCVTVAKTNKVVTSRSGWTYDGCYMCTKRVSILDGQLKCENGHVNNEPTPRYKVDIEVVYRNAKSRFVFFDGDCTQLLGISASDLRKKMIAAGQKDKRAYPYLFEKKLLNLTMVFRVKWSPTFKTSSVMRFSTDETFLSSVEDKLLKEETTGVPKIAYDSLQQSQNDHVQSVYPSATTEYDPENGSNTTPAKRLSSKEELTSDVVEETPTSKPGRKRFADNFQPCAVNDFCGTETSCTKKRKTTNWEEGTK*