AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0224110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0224110 MtrunA17_Chr2g0329931 99.752 404 1 0 1 404 1 404 0.0 832
Msa0224110 MtrunA17_Chr8g0342861 94.059 404 24 0 1 404 1 404 0.0 789
Msa0224110 MtrunA17_Chr2g0299681 93.069 404 28 0 1 404 1 404 0.0 785
Msa0224110 MtrunA17_Chr4g0070981 91.584 404 34 0 1 404 1 404 0.0 765
Msa0224110 MtrunA17_Chr2g0297261 64.560 364 129 0 41 404 34 397 6.62e-178 502
Msa0224110 MtrunA17_Chr1g0169331 61.299 385 112 5 56 404 6 389 8.67e-160 456
Msa0224110 MtrunA17_Chr8g0342851 62.908 337 93 4 71 404 3 310 2.35e-138 398
Msa0224110 MtrunA17_Chr7g0247181 41.854 356 203 4 42 396 141 493 1.25e-87 275
Msa0224110 MtrunA17_Chr7g0247221 41.854 356 203 4 42 396 144 496 1.61e-87 275
Msa0224110 MtrunA17_Chr7g0247191 42.135 356 202 4 42 396 151 503 1.94e-87 275
Msa0224110 MtrunA17_Chr4g0003441 39.362 376 221 7 35 404 101 475 7.21e-74 239
Msa0224110 MtrunA17_Chr1g0149251 39.157 332 196 6 78 404 1 331 1.58e-68 221
Msa0224110 MtrunA17_Chr3g0107851 35.467 375 220 5 40 396 116 486 3.44e-67 223
Msa0224110 MtrunA17_Chr1g0179761 36.432 398 213 13 41 404 80 471 3.62e-65 216
Msa0224110 MtrunA17_Chr2g0330401 35.068 365 223 3 40 394 95 455 1.12e-64 215
Msa0224110 MtrunA17_Chr7g0271121 38.728 346 189 8 66 396 131 468 1.78e-62 214
Msa0224110 MtrunA17_Chr4g0076491 34.301 379 217 7 47 397 293 667 1.07e-61 211
Msa0224110 MtrunA17_Chr7g0270131 34.530 362 230 4 40 394 231 592 2.20e-61 212
Msa0224110 MtrunA17_Chr6g0449741 34.748 377 216 7 47 397 255 627 4.67e-61 209
Msa0224110 MtrunA17_Chr3g0095031 32.512 406 251 6 11 396 48 450 1.36e-59 203
Msa0224110 MtrunA17_Chr3g0121351 33.500 400 243 7 41 418 145 543 3.28e-58 201
Msa0224110 MtrunA17_Chr1g0193491 33.607 366 223 8 45 396 118 477 4.70e-58 201
Msa0224110 MtrunA17_Chr6g0483491 33.241 361 237 3 40 397 9 368 1.08e-57 195
Msa0224110 MtrunA17_Chr5g0442191 34.349 361 224 6 45 396 85 441 2.07e-57 198
Msa0224110 MtrunA17_Chr7g0236841 33.241 361 237 3 40 397 9 368 2.64e-57 194
Msa0224110 MtrunA17_Chr2g0310581 32.987 385 249 3 41 420 53 433 2.34e-56 193
Msa0224110 MtrunA17_Chr2g0277821 35.230 369 223 6 40 396 503 867 2.25e-55 197
Msa0224110 MtrunA17_Chr2g0293631 33.511 376 239 5 41 410 496 866 2.63e-55 197
Msa0224110 MtrunA17_Chr4g0063551 33.333 399 237 7 42 414 141 536 3.96e-55 193
Msa0224110 MtrunA17_Chr4g0040761 32.884 371 239 5 42 405 20 387 6.23e-55 194
Msa0224110 MtrunA17_Chr1g0176041 33.249 394 232 8 40 406 153 542 3.52e-54 189
Msa0224110 MtrunA17_Chr1g0157691 31.667 360 228 7 40 384 146 502 6.62e-53 186
Msa0224110 MtrunA17_Chr1g0200181 31.667 360 228 7 40 384 144 500 7.65e-52 183
Msa0224110 MtrunA17_Chr6g0449911 32.055 365 237 5 41 397 165 526 2.04e-50 181
Msa0224110 MtrunA17_Chr3g0098121 32.812 384 239 7 27 396 135 513 2.74e-50 181
Msa0224110 MtrunA17_Chr5g0409981 33.803 355 221 5 42 384 105 457 4.92e-50 177
Msa0224110 MtrunA17_Chr6g0461751 31.978 369 229 7 41 394 140 501 5.72e-48 171
Msa0224110 MtrunA17_Chr2g0300291 31.593 383 246 7 27 396 124 503 9.86e-46 168
Msa0224110 MtrunA17_Chr2g0307471 31.522 368 229 12 40 391 90 450 7.97e-43 158
Msa0224110 MtrunA17_Chr4g0062301 30.726 358 226 10 50 391 106 457 4.37e-42 156
Msa0224110 MtrunA17_Chr6g0475251 31.054 351 215 6 45 380 115 453 5.16e-42 157
Msa0224110 MtrunA17_Chr7g0263771 28.142 366 256 4 34 397 129 489 8.49e-41 153
Msa0224110 MtrunA17_Chr4g0058591 31.595 326 213 4 24 343 189 510 4.08e-40 150
Msa0224110 MtrunA17_Chr2g0309551 25.234 428 255 7 40 405 108 532 4.39e-40 150
Msa0224110 MtrunA17_Chr3g0114081 29.396 364 229 7 47 383 22 384 3.11e-38 145
Msa0224110 MtrunA17_Chr1g0204391 28.926 363 223 5 49 380 52 410 8.88e-38 144
Msa0224110 MtrunA17_Chr8g0347171 29.888 358 232 9 42 380 161 518 1.84e-37 144
Msa0224110 MtrunA17_Chr8g0347161 29.330 358 234 8 42 380 103 460 6.63e-37 141
Msa0224110 MtrunA17_Chr7g0236831 26.077 441 236 10 41 399 163 595 1.12e-36 142
Msa0224110 MtrunA17_Chr8g0347151 29.050 358 235 7 42 380 202 559 6.63e-35 137
Msa0224110 MtrunA17_Chr4g0025141 37.379 206 114 5 214 404 14 219 1.56e-34 128
Msa0224110 MtrunA17_Chr8g0386451 27.297 381 242 7 42 395 289 661 3.79e-34 135
Msa0224110 MtrunA17_Chr1g0192021 26.406 409 246 8 41 397 26 431 2.29e-33 132
Msa0224110 MtrunA17_Chr6g0466611 28.312 385 238 11 41 396 142 517 7.32e-33 131
Msa0224110 MtrunA17_Chr4g0034411 28.947 342 224 10 68 395 102 438 1.67e-32 130
Msa0224110 MtrunA17_Chr5g0443991 27.528 356 245 8 46 390 81 434 2.46e-31 126
Msa0224110 MtrunA17_Chr3g0137901 39.623 159 94 2 247 404 10 167 2.24e-30 115
Msa0224110 MtrunA17_Chr5g0429221 27.174 368 248 7 40 396 2 360 7.90e-28 114
Msa0224110 MtrunA17_Chr3g0137891 42.236 161 90 3 78 235 1 161 3.06e-27 108
Msa0224110 MtrunA17_Chr3g0145781 26.933 401 239 13 24 383 120 507 2.82e-25 108
Msa0224110 MtrunA17_Chr4g0039471 36.126 191 119 1 36 223 218 408 4.88e-24 103
Msa0224110 MtrunA17_Chr4g0074521 25.310 403 245 14 49 409 90 478 1.45e-23 102
Msa0224110 MtrunA17_Chr4g0025131 34.270 178 111 4 24 197 40 215 2.12e-23 99.4
Msa0224110 MtrunA17_Chr4g0004301 23.499 383 214 6 79 389 71 446 1.49e-22 99.8
Msa0224110 MtrunA17_Chr4g0025101 31.624 234 94 8 198 407 7 198 2.02e-22 94.7
Msa0224110 MtrunA17_Chr4g0062321 29.237 236 157 6 117 348 4 233 3.78e-22 94.7
Msa0224110 MtrunA17_Chr5g0434301 23.238 383 215 6 79 389 71 446 8.01e-22 97.4
Msa0224110 MtrunA17_Chr8g0349951 36.429 140 82 2 40 175 9 145 3.76e-19 84.0
Msa0224110 MtrunA17_Chr4g0029191 31.285 179 114 4 41 210 44 222 1.08e-17 85.5
Msa0224110 MtrunA17_Chr4g0029191 34.653 101 66 0 310 410 392 492 8.19e-14 73.2
Msa0224110 MtrunA17_Chr1g0155201 28.571 273 156 12 152 408 6 255 2.41e-17 82.8
Msa0224110 MtrunA17_Chr2g0301281 30.726 179 115 4 41 210 41 219 3.37e-17 84.0
Msa0224110 MtrunA17_Chr2g0301281 35.632 87 56 0 310 396 389 475 9.00e-14 73.2
Msa0224110 MtrunA17_Chr8g0354761 23.889 360 244 13 64 404 31 379 6.28e-17 82.4
Msa0224110 MtrunA17_Chr4g0058601 47.170 53 28 0 344 396 9 61 8.68e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0224110 AT1G54270.1 92.840 405 26 2 1 404 1 403 0.0 774
Msa0224110 AT3G13920.1 92.346 405 28 2 1 404 1 403 0.0 769
Msa0224110 AT1G54270.2 91.605 405 26 3 1 404 1 398 0.0 755
Msa0224110 AT3G13920.4 91.111 405 28 3 1 404 1 398 0.0 753
Msa0224110 AT1G72730.1 89.136 405 43 1 1 404 1 405 0.0 751
Msa0224110 AT3G13920.5 92.366 393 27 2 1 392 5 395 0.0 744
Msa0224110 AT3G13920.2 92.366 393 27 2 1 392 1 391 0.0 744
Msa0224110 AT3G13920.3 94.195 379 21 1 27 404 15 393 0.0 737
Msa0224110 AT3G19760.1 62.632 380 135 1 25 404 27 399 5.01e-176 498
Msa0224110 AT1G51380.1 58.630 365 151 0 40 404 22 386 2.15e-160 457
Msa0224110 AT1G51380.2 58.103 253 106 0 40 292 22 274 1.24e-107 319
Msa0224110 AT3G61240.2 42.363 347 196 4 42 387 126 469 7.21e-88 276
Msa0224110 AT3G61240.1 42.363 347 196 4 42 387 126 469 7.21e-88 276
Msa0224110 AT2G45810.1 42.651 347 195 4 42 387 156 499 1.57e-87 276
Msa0224110 AT4G00660.1 41.011 356 206 4 42 396 133 485 1.10e-86 273
Msa0224110 AT4G00660.2 41.011 356 206 4 42 396 133 485 1.10e-86 273
Msa0224110 AT1G51380.4 55.660 212 94 0 40 251 22 233 2.43e-82 255
Msa0224110 AT1G51380.3 55.660 212 94 0 40 251 22 233 4.38e-82 254
Msa0224110 AT5G11170.1 38.642 383 228 7 28 404 33 414 7.90e-75 240
Msa0224110 AT5G11200.1 38.642 383 228 7 28 404 33 414 7.90e-75 240
Msa0224110 AT5G11170.2 39.157 332 196 6 78 404 1 331 2.18e-67 218
Msa0224110 AT5G11200.3 34.906 424 228 8 28 404 33 455 1.68e-66 219
Msa0224110 AT3G53110.1 35.000 400 233 10 26 404 81 474 1.81e-66 220
Msa0224110 AT1G55150.2 34.626 361 230 2 40 394 124 484 1.47e-65 218
Msa0224110 AT1G55150.1 34.626 361 230 2 40 394 99 459 2.48e-65 217
Msa0224110 AT5G26742.1 35.323 402 232 9 9 396 80 467 3.06e-63 217
Msa0224110 AT5G63120.2 35.544 377 227 4 42 409 167 536 3.07e-63 214
Msa0224110 AT5G26742.2 35.323 402 232 9 9 396 80 467 3.10e-63 217
Msa0224110 AT2G33730.1 34.987 383 221 6 41 397 314 694 6.73e-63 216
Msa0224110 AT5G26742.3 36.612 366 201 9 45 396 26 374 2.18e-62 213
Msa0224110 AT3G22310.2 35.457 361 220 6 45 396 121 477 1.20e-60 207
Msa0224110 AT3G22310.1 35.457 361 220 6 45 396 121 477 1.56e-60 207
Msa0224110 AT3G22330.1 35.180 361 221 6 45 396 109 465 1.63e-60 207
Msa0224110 AT2G47330.1 35.215 372 223 7 37 394 222 589 1.14e-58 204
Msa0224110 AT1G77030.1 32.698 367 239 4 42 402 30 394 3.44e-58 204
Msa0224110 AT3G58510.1 32.746 397 247 7 41 418 152 547 4.36e-58 201
Msa0224110 AT3G58510.3 32.746 397 247 7 41 418 152 547 4.36e-58 201
Msa0224110 AT3G58510.2 32.746 397 247 7 41 418 152 547 4.36e-58 201
Msa0224110 AT2G42520.2 35.075 402 231 10 33 406 149 548 5.05e-58 201
Msa0224110 AT2G42520.1 35.075 402 231 10 33 406 149 548 5.05e-58 201
Msa0224110 AT2G42520.3 35.075 402 231 10 33 406 149 548 5.05e-58 201
Msa0224110 AT1G20920.2 33.915 401 244 8 9 396 163 555 2.70e-57 202
Msa0224110 AT3G58570.1 33.252 409 250 8 33 418 136 544 5.03e-57 198
Msa0224110 AT3G06480.1 35.870 368 217 6 41 396 436 796 8.64e-57 202
Msa0224110 AT1G20920.4 35.262 363 219 6 46 396 535 893 2.33e-55 197
Msa0224110 AT1G20920.6 35.262 363 219 6 46 396 535 893 2.33e-55 197
Msa0224110 AT1G20920.3 35.262 363 219 6 46 396 535 893 2.33e-55 197
Msa0224110 AT1G20920.7 35.262 363 219 6 46 396 535 893 2.33e-55 197
Msa0224110 AT1G20920.5 35.262 363 219 6 46 396 535 893 2.33e-55 197
Msa0224110 AT1G20920.1 35.262 363 219 6 46 396 535 893 2.33e-55 197
Msa0224110 AT5G14610.5 33.607 366 230 6 41 397 170 531 3.03e-55 194
Msa0224110 AT5G14610.6 34.153 366 228 8 41 397 162 523 3.15e-55 193
Msa0224110 AT5G14610.2 34.153 366 228 8 41 397 162 523 3.65e-55 193
Msa0224110 AT1G16280.1 32.891 377 243 3 30 396 43 419 3.72e-55 190
Msa0224110 AT3G01540.1 35.792 366 220 10 41 396 159 519 7.07e-55 192
Msa0224110 AT3G01540.4 35.792 366 220 10 41 396 159 519 8.13e-55 192
Msa0224110 AT3G01540.2 35.792 366 220 10 41 396 159 519 8.13e-55 192
Msa0224110 AT3G01540.3 35.792 366 220 10 41 396 159 519 8.13e-55 192
Msa0224110 AT5G60990.1 32.632 380 232 5 36 397 5 378 8.77e-55 188
Msa0224110 AT4G16630.1 33.687 377 241 4 29 396 153 529 1.31e-53 191
Msa0224110 AT3G02065.3 32.603 365 233 5 41 394 111 473 3.33e-52 182
Msa0224110 AT3G02065.2 32.603 365 233 5 41 394 111 473 3.33e-52 182
Msa0224110 AT5G14610.7 33.048 351 222 6 55 396 45 391 6.56e-52 182
Msa0224110 AT5G14610.3 33.048 351 222 6 55 396 45 391 6.56e-52 182
Msa0224110 AT1G31970.1 33.799 358 222 5 40 384 116 471 6.27e-51 180
Msa0224110 AT1G28180.1 35.207 338 199 7 79 405 12 340 8.40e-51 176
Msa0224110 AT5G14610.1 33.144 353 223 6 53 396 242 590 1.40e-50 182
Msa0224110 AT5G11200.2 31.900 442 235 11 28 404 33 473 1.69e-50 177
Msa0224110 AT4G33370.1 31.944 360 227 7 40 384 97 453 2.09e-50 179
Msa0224110 AT5G51280.1 31.667 360 228 6 40 384 146 502 2.48e-50 179
Msa0224110 AT3G18600.1 32.184 348 227 5 41 380 90 436 1.07e-48 174
Msa0224110 AT3G09720.2 30.303 363 246 4 34 394 138 495 1.20e-47 171
Msa0224110 AT3G09720.1 30.303 363 246 4 34 394 138 495 1.20e-47 171
Msa0224110 AT3G09620.2 33.058 363 198 7 46 396 403 732 1.81e-47 174
Msa0224110 AT3G09620.1 33.058 363 198 7 46 396 402 731 1.84e-47 174
Msa0224110 AT3G02065.1 32.628 331 210 5 75 394 8 336 9.92e-47 165
Msa0224110 AT1G12770.2 27.295 403 229 7 40 381 110 509 1.06e-46 169
Msa0224110 AT1G12770.1 27.295 403 229 7 40 381 110 509 1.06e-46 169
Msa0224110 AT5G65900.1 30.667 375 251 6 38 404 152 525 3.93e-45 166
Msa0224110 AT5G62190.1 32.955 352 210 8 55 389 111 453 6.73e-45 166
Msa0224110 AT5G63120.3 33.435 329 203 4 24 343 153 474 2.87e-44 160
Msa0224110 AT5G63120.4 33.435 329 203 4 24 343 153 474 2.87e-44 160
Msa0224110 AT5G63120.5 33.435 329 203 4 24 343 153 474 2.87e-44 160
Msa0224110 AT5G63120.1 33.435 329 203 4 24 343 153 474 3.72e-44 160
Msa0224110 AT3G06480.2 32.551 341 207 7 24 352 423 752 7.04e-41 155
Msa0224110 AT5G05450.1 30.168 358 225 7 50 383 26 382 1.00e-40 153
Msa0224110 AT1G71370.1 30.114 352 230 7 47 383 23 373 5.23e-40 150
Msa0224110 AT5G14610.4 31.024 332 216 6 41 363 170 497 1.07e-39 149
Msa0224110 AT5G63630.1 31.006 358 228 7 42 380 56 413 6.19e-38 144
Msa0224110 AT5G63630.2 31.006 358 228 7 42 380 250 607 7.93e-37 143
Msa0224110 AT5G08620.1 29.921 381 232 8 42 395 82 454 8.14e-37 141
Msa0224110 AT5G08610.1 30.809 383 225 10 28 380 369 741 1.19e-36 143
Msa0224110 AT2G07750.1 28.338 367 227 9 42 380 377 735 1.57e-34 136
Msa0224110 AT5G19210.2 27.500 400 240 11 49 410 85 472 2.50e-33 130
Msa0224110 AT1G63250.1 28.297 364 225 8 42 377 330 685 4.47e-33 132
Msa0224110 AT4G34910.1 26.098 387 229 8 40 372 46 429 1.73e-31 127
Msa0224110 AT2G40700.1 25.277 451 256 12 41 410 31 481 2.94e-31 126
Msa0224110 AT5G54910.1 27.342 395 262 12 17 395 49 434 3.61e-31 126
Msa0224110 AT4G09730.1 26.753 385 246 9 40 396 112 488 1.36e-30 124
Msa0224110 AT3G06980.1 28.500 400 239 14 21 383 360 749 2.74e-27 115
Msa0224110 AT1G71280.2 27.419 310 189 9 47 345 24 308 3.78e-26 108
Msa0224110 AT4G15850.1 25.648 386 211 13 79 394 74 453 6.74e-26 110
Msa0224110 AT1G71280.1 27.419 310 189 9 47 345 24 308 2.87e-25 107
Msa0224110 AT3G16840.1 32.821 195 107 4 41 211 191 385 2.49e-19 91.3
Msa0224110 AT5G19210.1 26.074 326 191 11 123 410 2 315 2.54e-19 88.6
Msa0224110 AT5G19210.3 26.074 326 191 11 123 410 2 315 2.54e-19 88.6
Msa0224110 AT3G16840.2 32.821 195 107 4 41 211 192 386 2.57e-19 90.9
Msa0224110 AT1G28180.2 37.273 110 55 2 79 177 12 118 1.06e-11 62.4

Find 119 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCATAAGGTTTAAATTTA+AGG 0.096044 2_1:-73869814 None:intergenic
TGTTTAATCACTTAATTATT+AGG 0.102047 2_1:+73869748 Msa0224110:three_prime_UTR
GAACACTCCAACCTGAATTT+TGG 0.146444 2_1:-73868997 None:intergenic
TCGAAGAAAGGTTGAAAATT+AGG 0.156789 2_1:-73866739 None:intergenic
GAAGAGTGAGAGAGAGATTT+AGG 0.185365 2_1:-73866676 None:intergenic
TGACATGTATGATATAAATA+AGG 0.246109 2_1:-73869842 None:intergenic
TCTCATTACCACTGACCTTT+TGG 0.272997 2_1:+73869358 Msa0224110:CDS
GTTTAATCACTTAATTATTA+GGG 0.282354 2_1:+73869749 Msa0224110:three_prime_UTR
CGAAGAAAGGTTGAAAATTA+GGG 0.290793 2_1:-73866738 None:intergenic
CGGCGCAGATCCGAGGAGTT+TGG 0.291726 2_1:+73866711 Msa0224110:five_prime_UTR
ACATCTGACTCTATTTATAA+TGG 0.292865 2_1:+73869712 Msa0224110:three_prime_UTR
ACATCGAGACCCTTGATAAA+AGG 0.302219 2_1:-73868327 None:intergenic
TGCTGTGCCAGCTCCCTTGT+TGG 0.308505 2_1:-73868456 None:intergenic
TCAGTGCCAGGCTTTGGTTT+TGG 0.312026 2_1:+73868431 Msa0224110:CDS
GTTGCTGCCACCCAAAATTC+AGG 0.315611 2_1:+73868986 Msa0224110:CDS
GCTGTGCCAGCTCCCTTGTT+GGG 0.317476 2_1:-73868455 None:intergenic
TTGCATTAAAATGTTTGTTT+TGG 0.318028 2_1:+73868653 Msa0224110:CDS
CGCTTTACCAGAATTCTAAC+TGG 0.324777 2_1:-73869074 None:intergenic
CAGATGTCTTTGAACTCTAA+TGG 0.325903 2_1:-73869696 None:intergenic
AAGACAGCGACGTTCTGTTC+TGG 0.339543 2_1:+73868378 Msa0224110:CDS
TTGTTCTTTGTTAAGTCATC+GGG 0.340229 2_1:+73869615 Msa0224110:exon
TGGTGATTACCTTGGTGTTA+AGG 0.345997 2_1:+73868503 Msa0224110:CDS
TGATGATATCACGAGTGTTT+TGG 0.362515 2_1:-73869304 None:intergenic
GAATAAGCCAGTTAGAATTC+TGG 0.365648 2_1:+73869067 Msa0224110:CDS
GGCGCAGATCCGAGGAGTTT+GGG 0.371205 2_1:+73866712 Msa0224110:five_prime_UTR
CTGAGAGGCATATATGCTTA+TGG 0.371983 2_1:+73867704 Msa0224110:CDS
GTATCAAATTGTGAACCTTC+CGG 0.388344 2_1:-73867487 None:intergenic
TTATGGTTTGGTTCAGTGCC+AGG 0.388767 2_1:+73868419 Msa0224110:CDS
AAAACTATCTCCATCGTATT+GGG 0.393330 2_1:+73869444 Msa0224110:CDS
CAACAACATGGACACCAGTT+TGG 0.395614 2_1:-73868575 None:intergenic
CTTGTGATCTCCAGAGCCTC+TGG 0.397309 2_1:-73869035 None:intergenic
GCAGCAGCTTGATTATGGTT+TGG 0.397488 2_1:+73868407 Msa0224110:CDS
TTTGGTTCAGTGCCAGGCTT+TGG 0.398715 2_1:+73868425 Msa0224110:CDS
GAAAACTATCTCCATCGTAT+TGG 0.406801 2_1:+73869443 Msa0224110:CDS
ATTGGGCGAAGTGGGCGTTT+TGG 0.409374 2_1:+73869461 Msa0224110:CDS
AAATAAGGGTTGCTAGCATA+AGG 0.415054 2_1:-73869827 None:intergenic
AGATGTCTTTGAACTCTAAT+GGG 0.418418 2_1:-73869695 None:intergenic
CATGTTGTTGTTGGTACTCC+TGG 0.424537 2_1:+73868588 Msa0224110:CDS
AATGCTTTCACGTGGTTTCA+AGG 0.432515 2_1:+73868689 Msa0224110:CDS
CCCCTTTGCTGTATTGCAGA+TGG 0.433851 2_1:-73868297 None:intergenic
TTTGTTCTTTGTTAAGTCAT+CGG 0.435160 2_1:+73869614 Msa0224110:exon
GCCATCTGCAATACAGCAAA+GGG 0.435434 2_1:+73868296 Msa0224110:CDS
TTCATGAACTTCATCGTATG+AGG 0.438056 2_1:-73867648 None:intergenic
CGTGATGAATTGACCCTTGA+GGG 0.439342 2_1:+73869095 Msa0224110:CDS
GAGAGAATTGAAGAGAGAAT+AGG 0.441445 2_1:-73866763 None:intergenic
CATGGGTGGCTGAGACAGTA+TGG 0.446776 2_1:-73869271 None:intergenic
TGATGTTCAGCAAGTGTCTC+TGG 0.448249 2_1:+73869391 Msa0224110:CDS
CAGCAGGCTCAGTCAGGAAC+TGG 0.451120 2_1:+73868354 Msa0224110:CDS
GATCTGCGCCGTGGAGTTGA+AGG 0.453865 2_1:-73866699 None:intergenic
TACAATGCCTCCAGAGGCTC+TGG 0.459621 2_1:+73869025 Msa0224110:CDS
CATCAATACCACGGGCCAAA+AGG 0.460041 2_1:-73869373 None:intergenic
TTTGGCCCCAACAAGGGAGC+TGG 0.463851 2_1:+73868449 Msa0224110:CDS
AAGGTTCATGCTTGTGTTGG+TGG 0.466235 2_1:+73868522 Msa0224110:CDS
AGTAGATCAGCAACATTTGA+AGG 0.475461 2_1:-73869577 None:intergenic
TGCTGCGAAGACAGTCTCTT+CGG 0.488354 2_1:+73868625 Msa0224110:CDS
GTGATTCAGCAGGCTCAGTC+AGG 0.498167 2_1:+73868348 Msa0224110:CDS
CATCTTAGTGTCAAACTGCT+TGG 0.499196 2_1:-73867509 None:intergenic
CTCCATCGTATTGGGCGAAG+TGG 0.499221 2_1:+73869452 Msa0224110:CDS
ACTGGTGTCCATGTTGTTGT+TGG 0.499852 2_1:+73868579 Msa0224110:CDS
GATCAGCGTATTCTCCAAAC+TGG 0.501639 2_1:+73868561 Msa0224110:CDS
TTCACGTGGTTTCAAGGACC+AGG 0.504125 2_1:+73868695 Msa0224110:CDS
ACACAAGGCGCAAGGTGGAC+TGG 0.505122 2_1:+73869222 Msa0224110:CDS
GTTAAGGTTCATGCTTGTGT+TGG 0.507288 2_1:+73868519 Msa0224110:CDS
GTGTGATCTTTATGAGACTC+TGG 0.521304 2_1:+73869172 Msa0224110:CDS
ATCATGCGTGAATTCCGCTC+TGG 0.527239 2_1:+73869323 Msa0224110:CDS
GTGTATATTCTACTACTACT+AGG 0.530544 2_1:-73866621 None:intergenic
GAAGAATTCTTGTCCTTCAG+TGG 0.531706 2_1:-73867624 None:intergenic
GAGAAGGTTATGAGAGCACT+TGG 0.533228 2_1:+73868483 Msa0224110:CDS
AATTACAGGCTTTCCACTGA+AGG 0.540134 2_1:+73867611 Msa0224110:intron
GAGAGAATAGGATCGAAGAA+AGG 0.540291 2_1:-73866751 None:intergenic
CTGTGCCAGCTCCCTTGTTG+GGG 0.543113 2_1:-73868454 None:intergenic
TGCTATCAACTTCGTCACAT+TGG 0.543489 2_1:+73869496 Msa0224110:CDS
AGCATGTCAAACACACGTCC+AGG 0.544974 2_1:-73868606 None:intergenic
GGGTCTCGATGTGATTCAGC+AGG 0.549184 2_1:+73868338 Msa0224110:CDS
CTCTGCTACAATGCCTCCAG+AGG 0.551425 2_1:+73869019 Msa0224110:CDS
TTAGGGTTTCCCAAACTCCT+CGG 0.552128 2_1:-73866721 None:intergenic
GACATGTATGATATAAATAA+GGG 0.554033 2_1:-73869841 None:intergenic
TTGGGGCCAAAACCAAAGCC+TGG 0.554251 2_1:-73868437 None:intergenic
TCTTCGCTCACTCCACAAGC+AGG 0.561786 2_1:+73866787 Msa0224110:five_prime_UTR
CAGAGGCTCTGGAGATCACA+AGG 0.564855 2_1:+73869036 Msa0224110:CDS
CTCAGCCACCCATGGAGACA+TGG 0.565513 2_1:+73869280 Msa0224110:CDS
AACTCCACGGCGCAGATCCG+AGG 0.568103 2_1:+73866704 Msa0224110:five_prime_UTR
TCCATCGTATTGGGCGAAGT+GGG 0.570459 2_1:+73869453 Msa0224110:CDS
CTGCCACCCAAAATTCAGGT+TGG 0.574116 2_1:+73868990 Msa0224110:CDS
TTGGTCCATGTCTCCATGGG+TGG 0.574389 2_1:-73869285 None:intergenic
AGTCATGGCAGGTTTAGCTC+CGG 0.585504 2_1:+73867468 Msa0224110:intron
AGCCATCTGCAATACAGCAA+AGG 0.592072 2_1:+73868295 Msa0224110:CDS
CACTCTTCCCTTCAACTCCA+CGG 0.594775 2_1:+73866691 Msa0224110:five_prime_UTR
AACTCCTCGGATCTGCGCCG+TGG 0.597296 2_1:-73866708 None:intergenic
TTCTTTGTTAAGTCATCGGG+GGG 0.598798 2_1:+73869618 Msa0224110:exon
ATTGGAAGATGCAAGAATGC+TGG 0.599752 2_1:+73869514 Msa0224110:CDS
GCCCACTTCGCCCAATACGA+TGG 0.601432 2_1:-73869454 None:intergenic
AGAACTGCTTGATACCCTCA+AGG 0.605423 2_1:-73869109 None:intergenic
CAGGAGTACCAACAACAACA+TGG 0.608453 2_1:-73868587 None:intergenic
GTGATCTCCAGAGCCTCTGG+AGG 0.613832 2_1:-73869032 None:intergenic
ATCTGCGCCGTGGAGTTGAA+GGG 0.615084 2_1:-73866698 None:intergenic
TGAATGTTGACAAGGAAGAG+TGG 0.615932 2_1:+73869135 Msa0224110:CDS
GTTCTTTGTTAAGTCATCGG+GGG 0.616041 2_1:+73869617 Msa0224110:exon
TTGCTGAACATCAATACCAC+GGG 0.620577 2_1:-73869381 None:intergenic
GAGATACGTATACCTGCTTG+TGG 0.623092 2_1:-73866799 None:intergenic
GCTGGCACAGCAGATTGAGA+AGG 0.628451 2_1:+73868467 Msa0224110:CDS
CATACTGTCTCAGCCACCCA+TGG 0.631002 2_1:+73869272 Msa0224110:CDS
ATGGCAGGTTTAGCTCCGGA+AGG 0.641220 2_1:+73867472 Msa0224110:CDS
AGAGCACTTGGTGATTACCT+TGG 0.644049 2_1:+73868495 Msa0224110:CDS
TGTTCTTTGTTAAGTCATCG+GGG 0.645741 2_1:+73869616 Msa0224110:exon
GTTCTATAATGTGATCGTAG+AGG 0.657829 2_1:+73869550 Msa0224110:CDS
CTTGCTGAACATCAATACCA+CGG 0.663030 2_1:-73869382 None:intergenic
GCGTGATGAATTGACCCTTG+AGG 0.663843 2_1:+73869094 Msa0224110:CDS
ACCACGGGCCAAAAGGTCAG+TGG 0.665992 2_1:-73869366 None:intergenic
TTGATAGAAAATCTTCTGAG+AGG 0.671045 2_1:+73867689 Msa0224110:CDS
GAACTGCTTGATACCCTCAA+GGG 0.677590 2_1:-73869108 None:intergenic
TGGCTCACTGACAAGATGCG+AGG 0.683723 2_1:+73869242 Msa0224110:CDS
TGGTGGCACTAGTGTTCGTG+AGG 0.685653 2_1:+73868539 Msa0224110:CDS
GTTCTACGTGAATGTTGACA+AGG 0.687466 2_1:+73869127 Msa0224110:CDS
GAACTCGAGAAGAGCCAGAG+CGG 0.689835 2_1:-73869337 None:intergenic
TGTGAACACAAGGCGCAAGG+TGG 0.709342 2_1:+73869217 Msa0224110:CDS
AAGCATGAACCTTAACACCA+AGG 0.715621 2_1:-73868512 None:intergenic
GCTGATGAAATGCTTTCACG+TGG 0.720414 2_1:+73868681 Msa0224110:CDS
CCATCTGCAATACAGCAAAG+GGG 0.725959 2_1:+73868297 Msa0224110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTTTTTTAAATCA+TGG + chr2_1:73867287-73867306 Msa0224110:intron 10.0%
!! CAAAAGTTTAAAAATTAATT+GGG + chr2_1:73867844-73867863 Msa0224110:intron 10.0%
!! AATCTAAAAGAATGAAAATT+GGG - chr2_1:73866840-73866859 None:intergenic 15.0%
!! AAATCTAAAAGAATGAAAAT+TGG - chr2_1:73866841-73866860 None:intergenic 15.0%
!!! TTTTTTTTTTGTAAATTTGG+AGG + chr2_1:73867245-73867264 Msa0224110:intron 15.0%
!!! TAATGCATAATTTTAATTAC+AGG + chr2_1:73867597-73867616 Msa0224110:intron 15.0%
!! GCAAAAGTTTAAAAATTAAT+TGG + chr2_1:73867843-73867862 Msa0224110:intron 15.0%
!! TGTTTAATCACTTAATTATT+AGG + chr2_1:73869748-73869767 Msa0224110:three_prime_UTR 15.0%
!! GTTTAATCACTTAATTATTA+GGG + chr2_1:73869749-73869768 Msa0224110:three_prime_UTR 15.0%
!! ATCTAAAAGAATGAAAATTG+GGG - chr2_1:73866839-73866858 None:intergenic 20.0%
!!! TATTAGTTTGTTTTGATTTG+TGG + chr2_1:73866994-73867013 Msa0224110:intron 20.0%
!!! ATTGATTTTTGCTTTTGTTT+TGG - chr2_1:73867083-73867102 None:intergenic 20.0%
!! TAATAAGGCTTAAAGAAAAA+AGG - chr2_1:73867746-73867765 None:intergenic 20.0%
!! AAGGATTAAAAAATTGTGAT+AGG - chr2_1:73867820-73867839 None:intergenic 20.0%
!!! TCACAATTTTTTAATCCTTT+TGG + chr2_1:73867821-73867840 Msa0224110:intron 20.0%
!!! ATTTTTAAACTTTTGCCAAA+AGG - chr2_1:73867839-73867858 None:intergenic 20.0%
!!! TGAAAACTCTTTAAAAATCA+TGG + chr2_1:73867886-73867905 Msa0224110:intron 20.0%
!! GCAATTAATTAATTAACTCA+AGG + chr2_1:73867935-73867954 Msa0224110:intron 20.0%
!! ATTAACTCAAGGTTATATAA+TGG + chr2_1:73867946-73867965 Msa0224110:intron 20.0%
!! TTAACTCAAGGTTATATAAT+GGG + chr2_1:73867947-73867966 Msa0224110:intron 20.0%
!! GTATATATAAACAATTTCGA+GGG + chr2_1:73868022-73868041 Msa0224110:intron 20.0%
!!! TTGCATTAAAATGTTTGTTT+TGG + chr2_1:73868653-73868672 Msa0224110:CDS 20.0%
!! CACAAAACAGAAATAAAAAA+AGG - chr2_1:73869784-73869803 None:intergenic 20.0%
!! TAGCATAAGGTTTAAATTTA+AGG - chr2_1:73869817-73869836 None:intergenic 20.0%
!! GACATGTATGATATAAATAA+GGG - chr2_1:73869844-73869863 None:intergenic 20.0%
!! TGACATGTATGATATAAATA+AGG - chr2_1:73869845-73869864 None:intergenic 20.0%
! TGATTTCAAAAATCAGAACT+TGG - chr2_1:73866943-73866962 None:intergenic 25.0%
! ATGAGATGATTCAAATCTTA+AGG - chr2_1:73867149-73867168 None:intergenic 25.0%
! TTAAGATTTGAATCATCTCA+TGG + chr2_1:73867148-73867167 Msa0224110:intron 25.0%
! CAAAAATAAACCAACAACAA+AGG - chr2_1:73867383-73867402 None:intergenic 25.0%
! AGATGAATGAATTGTAAGTA+AGG + chr2_1:73867527-73867546 Msa0224110:intron 25.0%
! GTTATCATACATGCTTAATA+AGG - chr2_1:73867761-73867780 None:intergenic 25.0%
! TTTAAAAATTAATTGGGTGC+TGG + chr2_1:73867850-73867869 Msa0224110:intron 25.0%
!!! ATGGACATTTATTTTTAGAC+AGG + chr2_1:73867905-73867924 Msa0224110:intron 25.0%
!!! TGGACATTTATTTTTAGACA+GGG + chr2_1:73867906-73867925 Msa0224110:intron 25.0%
! ATATAGTGACAACTATCAAA+GGG - chr2_1:73867983-73868002 None:intergenic 25.0%
! TATATAGTGACAACTATCAA+AGG - chr2_1:73867984-73868003 None:intergenic 25.0%
! GGTATATATAAACAATTTCG+AGG + chr2_1:73868021-73868040 Msa0224110:intron 25.0%
! AGGTTAAAACGTAATAAGAA+CGG - chr2_1:73868152-73868171 None:intergenic 25.0%
!! TTGTTTGTTAGAACTGAATT+TGG + chr2_1:73868771-73868790 Msa0224110:intron 25.0%
! TAAAACAAGATCAATTCATC+TGG - chr2_1:73868907-73868926 None:intergenic 25.0%
! TTTGTTCTTTGTTAAGTCAT+CGG + chr2_1:73869614-73869633 Msa0224110:exon 25.0%
! ACATCTGACTCTATTTATAA+TGG + chr2_1:73869712-73869731 Msa0224110:three_prime_UTR 25.0%
GTGTATATTCTACTACTACT+AGG - chr2_1:73866624-73866643 None:intergenic 30.0%
CGAAGAAAGGTTGAAAATTA+GGG - chr2_1:73866741-73866760 None:intergenic 30.0%
TCGAAGAAAGGTTGAAAATT+AGG - chr2_1:73866742-73866761 None:intergenic 30.0%
!!! GTTTGTTTTGATTTGTGGTA+AGG + chr2_1:73866999-73867018 Msa0224110:intron 30.0%
!!! AGGATTTGTTGTTTTCTGAT+TGG + chr2_1:73867171-73867190 Msa0224110:intron 30.0%
CAAGAAAACACATAACACAA+GGG - chr2_1:73867342-73867361 None:intergenic 30.0%
AGAACAAAACAGTACACTTT+AGG - chr2_1:73867446-73867465 None:intergenic 30.0%
!! ACTGTTTTGTTCTATAGTCA+TGG + chr2_1:73867453-73867472 Msa0224110:intron 30.0%
! TCATGAAACTTTTGATGCAA+TGG + chr2_1:73867664-73867683 Msa0224110:CDS 30.0%
! CATGAAACTTTTGATGCAAT+GGG + chr2_1:73867665-73867684 Msa0224110:CDS 30.0%
TTGATAGAAAATCTTCTGAG+AGG + chr2_1:73867689-73867708 Msa0224110:CDS 30.0%
AATTGTGATAGGCACTAATT+GGG - chr2_1:73867809-73867828 None:intergenic 30.0%
AAATTGTGATAGGCACTAAT+TGG - chr2_1:73867810-73867829 None:intergenic 30.0%
!!! GGACATTTATTTTTAGACAG+GGG + chr2_1:73867907-73867926 Msa0224110:intron 30.0%
!!! GACATTTATTTTTAGACAGG+GGG + chr2_1:73867908-73867927 Msa0224110:intron 30.0%
TATATACCAATGACAAGCAT+GGG - chr2_1:73868009-73868028 None:intergenic 30.0%
ATATATACCAATGACAAGCA+TGG - chr2_1:73868010-73868029 None:intergenic 30.0%
! CTTTTCTTTAATCGCATGTT+CGG + chr2_1:73868054-73868073 Msa0224110:intron 30.0%
CTACGTGTATAGATGTATTT+AGG - chr2_1:73868172-73868191 None:intergenic 30.0%
! GGAATTGTTCCTTTTATCAA+GGG + chr2_1:73868318-73868337 Msa0224110:CDS 30.0%
TGTGTTAGATTTCTGATTGT+TGG + chr2_1:73868718-73868737 Msa0224110:intron 30.0%
! AAGGTAAAGGGTATAGAATA+TGG - chr2_1:73868866-73868885 None:intergenic 30.0%
AAAACTATCTCCATCGTATT+GGG + chr2_1:73869444-73869463 Msa0224110:CDS 30.0%
TTGTTCTTTGTTAAGTCATC+GGG + chr2_1:73869615-73869634 Msa0224110:exon 30.0%
AGATGTCTTTGAACTCTAAT+GGG - chr2_1:73869698-73869717 None:intergenic 30.0%
GAGAGAATTGAAGAGAGAAT+AGG - chr2_1:73866766-73866785 None:intergenic 35.0%
AGATTTGAATCATCTCATGG+AGG + chr2_1:73867151-73867170 Msa0224110:intron 35.0%
GCAAGAAAACACATAACACA+AGG - chr2_1:73867343-73867362 None:intergenic 35.0%
! TGTTTTCTTGCAGTGATATC+TGG + chr2_1:73867352-73867371 Msa0224110:intron 35.0%
! GTTTTCTTGCAGTGATATCT+GGG + chr2_1:73867353-73867372 Msa0224110:intron 35.0%
! TTTTCTTGCAGTGATATCTG+GGG + chr2_1:73867354-73867373 Msa0224110:intron 35.0%
! TTTTGTTCTATAGTCATGGC+AGG + chr2_1:73867457-73867476 Msa0224110:intron 35.0%
GTATCAAATTGTGAACCTTC+CGG - chr2_1:73867490-73867509 None:intergenic 35.0%
TTCATGAACTTCATCGTATG+AGG - chr2_1:73867651-73867670 None:intergenic 35.0%
ATATACCAATGACAAGCATG+GGG - chr2_1:73868008-73868027 None:intergenic 35.0%
ATGTTCTGTTCTTGTGCTTA+AGG + chr2_1:73868264-73868283 Msa0224110:intron 35.0%
! GGGAATTGTTCCTTTTATCA+AGG + chr2_1:73868317-73868336 Msa0224110:CDS 35.0%
! ATTTTGCAGCAGCTTGATTA+TGG + chr2_1:73868402-73868421 Msa0224110:CDS 35.0%
AATCAGAAATCTAACACACC+TGG - chr2_1:73868716-73868735 None:intergenic 35.0%
CTGATTGTTGGAACTGAATT+TGG + chr2_1:73868730-73868749 Msa0224110:intron 35.0%
TTGTTGGAACTGAATTTGGT+TGG + chr2_1:73868734-73868753 Msa0224110:intron 35.0%
AACTGAATTTGGTTGGAGTT+GGG + chr2_1:73868741-73868760 Msa0224110:intron 35.0%
GTAGGTCAAATGAAGGTAAA+GGG - chr2_1:73868878-73868897 None:intergenic 35.0%
AGTAGGTCAAATGAAGGTAA+AGG - chr2_1:73868879-73868898 None:intergenic 35.0%
! AACACTCCAACCTGAATTTT+GGG - chr2_1:73868999-73869018 None:intergenic 35.0%
GAATAAGCCAGTTAGAATTC+TGG + chr2_1:73869067-73869086 Msa0224110:CDS 35.0%
AAAAATGACACTCTGAGTGA+TGG - chr2_1:73869198-73869217 None:intergenic 35.0%
!!! GTGTCATTTTTGTGAACACA+AGG + chr2_1:73869207-73869226 Msa0224110:CDS 35.0%
! TGATGATATCACGAGTGTTT+TGG - chr2_1:73869307-73869326 None:intergenic 35.0%
!!! ATGGAGATAGTTTTCAGGTT+GGG - chr2_1:73869438-73869457 None:intergenic 35.0%
! ATACGATGGAGATAGTTTTC+AGG - chr2_1:73869443-73869462 None:intergenic 35.0%
GAAAACTATCTCCATCGTAT+TGG + chr2_1:73869443-73869462 Msa0224110:CDS 35.0%
GTTCTATAATGTGATCGTAG+AGG + chr2_1:73869550-73869569 Msa0224110:CDS 35.0%
AGTAGATCAGCAACATTTGA+AGG - chr2_1:73869580-73869599 None:intergenic 35.0%
TGTTCTTTGTTAAGTCATCG+GGG + chr2_1:73869616-73869635 Msa0224110:exon 35.0%
CAGATGTCTTTGAACTCTAA+TGG - chr2_1:73869699-73869718 None:intergenic 35.0%
AAATAAGGGTTGCTAGCATA+AGG - chr2_1:73869830-73869849 None:intergenic 35.0%
GAAGAGTGAGAGAGAGATTT+AGG - chr2_1:73866679-73866698 None:intergenic 40.0%
GAGAGAATAGGATCGAAGAA+AGG - chr2_1:73866754-73866773 None:intergenic 40.0%
CATCTTAGTGTCAAACTGCT+TGG - chr2_1:73867512-73867531 None:intergenic 40.0%
AATTACAGGCTTTCCACTGA+AGG + chr2_1:73867611-73867630 Msa0224110:intron 40.0%
GAAGAATTCTTGTCCTTCAG+TGG - chr2_1:73867627-73867646 None:intergenic 40.0%
! CTGAGAGGCATATATGCTTA+TGG + chr2_1:73867704-73867723 Msa0224110:CDS 40.0%
!!! TTATTTTTAGACAGGGGGCT+TGG + chr2_1:73867913-73867932 Msa0224110:intron 40.0%
CAAGAACAGAACATCACACA+TGG - chr2_1:73868258-73868277 None:intergenic 40.0%
ACATCGAGACCCTTGATAAA+AGG - chr2_1:73868330-73868349 None:intergenic 40.0%
!! TGGTGATTACCTTGGTGTTA+AGG + chr2_1:73868503-73868522 Msa0224110:CDS 40.0%
AAGCATGAACCTTAACACCA+AGG - chr2_1:73868515-73868534 None:intergenic 40.0%
GTTAAGGTTCATGCTTGTGT+TGG + chr2_1:73868519-73868538 Msa0224110:CDS 40.0%
AATGCTTTCACGTGGTTTCA+AGG + chr2_1:73868689-73868708 Msa0224110:CDS 40.0%
GAACTGAATTTGGTTGGAGT+TGG + chr2_1:73868740-73868759 Msa0224110:intron 40.0%
GTGAGAAGTAGGTCAAATGA+AGG - chr2_1:73868885-73868904 None:intergenic 40.0%
CAATTCATCTGGTGAGAAGT+AGG - chr2_1:73868896-73868915 None:intergenic 40.0%
! GAACACTCCAACCTGAATTT+TGG - chr2_1:73869000-73869019 None:intergenic 40.0%
CGCTTTACCAGAATTCTAAC+TGG - chr2_1:73869077-73869096 None:intergenic 40.0%
GTTCTACGTGAATGTTGACA+AGG + chr2_1:73869127-73869146 Msa0224110:CDS 40.0%
TGAATGTTGACAAGGAAGAG+TGG + chr2_1:73869135-73869154 Msa0224110:CDS 40.0%
GTGTGATCTTTATGAGACTC+TGG + chr2_1:73869172-73869191 Msa0224110:CDS 40.0%
! TCTCATTACCACTGACCTTT+TGG + chr2_1:73869358-73869377 Msa0224110:CDS 40.0%
TTGCTGAACATCAATACCAC+GGG - chr2_1:73869384-73869403 None:intergenic 40.0%
CTTGCTGAACATCAATACCA+CGG - chr2_1:73869385-73869404 None:intergenic 40.0%
!!! AGATAGTTTTCAGGTTGGGT+AGG - chr2_1:73869434-73869453 None:intergenic 40.0%
!!! GATGGAGATAGTTTTCAGGT+TGG - chr2_1:73869439-73869458 None:intergenic 40.0%
TGCTATCAACTTCGTCACAT+TGG + chr2_1:73869496-73869515 Msa0224110:CDS 40.0%
ATTGGAAGATGCAAGAATGC+TGG + chr2_1:73869514-73869533 Msa0224110:CDS 40.0%
GTTCTTTGTTAAGTCATCGG+GGG + chr2_1:73869617-73869636 Msa0224110:exon 40.0%
TTCTTTGTTAAGTCATCGGG+GGG + chr2_1:73869618-73869637 Msa0224110:exon 40.0%
TTAGGGTTTCCCAAACTCCT+CGG - chr2_1:73866724-73866743 None:intergenic 45.0%
GAGATACGTATACCTGCTTG+TGG - chr2_1:73866802-73866821 None:intergenic 45.0%
TATAGCCCCATGCTTGTCAT+TGG + chr2_1:73868000-73868019 Msa0224110:intron 45.0%
TCACACATGGTATGCTAGTG+AGG - chr2_1:73868245-73868264 None:intergenic 45.0%
AGCCATCTGCAATACAGCAA+AGG + chr2_1:73868295-73868314 Msa0224110:CDS 45.0%
GCCATCTGCAATACAGCAAA+GGG + chr2_1:73868296-73868315 Msa0224110:CDS 45.0%
CCATCTGCAATACAGCAAAG+GGG + chr2_1:73868297-73868316 Msa0224110:CDS 45.0%
GCAGCAGCTTGATTATGGTT+TGG + chr2_1:73868407-73868426 Msa0224110:CDS 45.0%
!! TTATGGTTTGGTTCAGTGCC+AGG + chr2_1:73868419-73868438 Msa0224110:CDS 45.0%
!!! TTTGGTTTTGGCCCCAACAA+GGG + chr2_1:73868443-73868462 Msa0224110:CDS 45.0%
!! GAGAAGGTTATGAGAGCACT+TGG + chr2_1:73868483-73868502 Msa0224110:CDS 45.0%
! AGAGCACTTGGTGATTACCT+TGG + chr2_1:73868495-73868514 Msa0224110:CDS 45.0%
AAGGTTCATGCTTGTGTTGG+TGG + chr2_1:73868522-73868541 Msa0224110:CDS 45.0%
GATCAGCGTATTCTCCAAAC+TGG + chr2_1:73868561-73868580 Msa0224110:CDS 45.0%
CAACAACATGGACACCAGTT+TGG - chr2_1:73868578-73868597 None:intergenic 45.0%
ACTGGTGTCCATGTTGTTGT+TGG + chr2_1:73868579-73868598 Msa0224110:CDS 45.0%
!! CAGGAGTACCAACAACAACA+TGG - chr2_1:73868590-73868609 None:intergenic 45.0%
! CATGTTGTTGTTGGTACTCC+TGG + chr2_1:73868588-73868607 Msa0224110:CDS 45.0%
GCTGATGAAATGCTTTCACG+TGG + chr2_1:73868681-73868700 Msa0224110:CDS 45.0%
! ACTCCAACCTGAATTTTGGG+TGG - chr2_1:73868996-73869015 None:intergenic 45.0%
CGTGATGAATTGACCCTTGA+GGG + chr2_1:73869095-73869114 Msa0224110:CDS 45.0%
GAACTGCTTGATACCCTCAA+GGG - chr2_1:73869111-73869130 None:intergenic 45.0%
AGAACTGCTTGATACCCTCA+AGG - chr2_1:73869112-73869131 None:intergenic 45.0%
! TTTTGTGAACACAAGGCGCA+AGG + chr2_1:73869214-73869233 Msa0224110:CDS 45.0%
!! GTTTTGGTCCATGTCTCCAT+GGG - chr2_1:73869291-73869310 None:intergenic 45.0%
!!! TGTTTTGGTCCATGTCTCCA+TGG - chr2_1:73869292-73869311 None:intergenic 45.0%
TGATGTTCAGCAAGTGTCTC+TGG + chr2_1:73869391-73869410 Msa0224110:CDS 45.0%
!!! AGTGGGCGTTTTGGAAGAAA+AGG + chr2_1:73869470-73869489 Msa0224110:CDS 45.0%
!!! TTTTTTTTTTTTTGTAAATT+TGG + chr2_1:73867242-73867261 Msa0224110:intron 5.0%
CACTCTTCCCTTCAACTCCA+CGG + chr2_1:73866691-73866710 Msa0224110:five_prime_UTR 50.0%
! TCTGGGGATGCCTTTGTTGT+TGG + chr2_1:73867370-73867389 Msa0224110:intron 50.0%
! AGTCATGGCAGGTTTAGCTC+CGG + chr2_1:73867468-73867487 Msa0224110:intron 50.0%
! CCCCTTTGCTGTATTGCAGA+TGG - chr2_1:73868300-73868319 None:intergenic 50.0%
AAGACAGCGACGTTCTGTTC+TGG + chr2_1:73868378-73868397 Msa0224110:CDS 50.0%
! TTTGGTTCAGTGCCAGGCTT+TGG + chr2_1:73868425-73868444 Msa0224110:CDS 50.0%
!! TCAGTGCCAGGCTTTGGTTT+TGG + chr2_1:73868431-73868450 Msa0224110:CDS 50.0%
!!! CTTTGGTTTTGGCCCCAACA+AGG + chr2_1:73868442-73868461 Msa0224110:CDS 50.0%
AGCATGTCAAACACACGTCC+AGG - chr2_1:73868609-73868628 None:intergenic 50.0%
TGCTGCGAAGACAGTCTCTT+CGG + chr2_1:73868625-73868644 Msa0224110:CDS 50.0%
TTCACGTGGTTTCAAGGACC+AGG + chr2_1:73868695-73868714 Msa0224110:CDS 50.0%
!! GAATTTTGGGTGGCAGCAAC+TGG - chr2_1:73868986-73869005 None:intergenic 50.0%
GTTGCTGCCACCCAAAATTC+AGG + chr2_1:73868986-73869005 Msa0224110:CDS 50.0%
CTGCCACCCAAAATTCAGGT+TGG + chr2_1:73868990-73869009 Msa0224110:CDS 50.0%
GCGTGATGAATTGACCCTTG+AGG + chr2_1:73869094-73869113 Msa0224110:CDS 50.0%
ATCATGCGTGAATTCCGCTC+TGG + chr2_1:73869323-73869342 Msa0224110:CDS 50.0%
CATCAATACCACGGGCCAAA+AGG - chr2_1:73869376-73869395 None:intergenic 50.0%
TCCATCGTATTGGGCGAAGT+GGG + chr2_1:73869453-73869472 Msa0224110:CDS 50.0%
ATCTGCGCCGTGGAGTTGAA+GGG - chr2_1:73866701-73866720 None:intergenic 55.0%
TCTTCGCTCACTCCACAAGC+AGG + chr2_1:73866787-73866806 Msa0224110:five_prime_UTR 55.0%
! ATGGCAGGTTTAGCTCCGGA+AGG + chr2_1:73867472-73867491 Msa0224110:CDS 55.0%
GGGTCTCGATGTGATTCAGC+AGG + chr2_1:73868338-73868357 Msa0224110:CDS 55.0%
GTGATTCAGCAGGCTCAGTC+AGG + chr2_1:73868348-73868367 Msa0224110:CDS 55.0%
TTGGGGCCAAAACCAAAGCC+TGG - chr2_1:73868440-73868459 None:intergenic 55.0%
GCTGGCACAGCAGATTGAGA+AGG + chr2_1:73868467-73868486 Msa0224110:CDS 55.0%
TGGTGGCACTAGTGTTCGTG+AGG + chr2_1:73868539-73868558 Msa0224110:CDS 55.0%
CTCTGCTACAATGCCTCCAG+AGG + chr2_1:73869019-73869038 Msa0224110:CDS 55.0%
TACAATGCCTCCAGAGGCTC+TGG + chr2_1:73869025-73869044 Msa0224110:CDS 55.0%
CTTGTGATCTCCAGAGCCTC+TGG - chr2_1:73869038-73869057 None:intergenic 55.0%
!! CAGAGGCTCTGGAGATCACA+AGG + chr2_1:73869036-73869055 Msa0224110:CDS 55.0%
TGTGAACACAAGGCGCAAGG+TGG + chr2_1:73869217-73869236 Msa0224110:CDS 55.0%
TGGCTCACTGACAAGATGCG+AGG + chr2_1:73869242-73869261 Msa0224110:CDS 55.0%
CATGGGTGGCTGAGACAGTA+TGG - chr2_1:73869274-73869293 None:intergenic 55.0%
CATACTGTCTCAGCCACCCA+TGG + chr2_1:73869272-73869291 Msa0224110:CDS 55.0%
TTGGTCCATGTCTCCATGGG+TGG - chr2_1:73869288-73869307 None:intergenic 55.0%
GAACTCGAGAAGAGCCAGAG+CGG - chr2_1:73869340-73869359 None:intergenic 55.0%
CTCCATCGTATTGGGCGAAG+TGG + chr2_1:73869452-73869471 Msa0224110:CDS 55.0%
! ATTGGGCGAAGTGGGCGTTT+TGG + chr2_1:73869461-73869480 Msa0224110:CDS 55.0%
GATCTGCGCCGTGGAGTTGA+AGG - chr2_1:73866702-73866721 None:intergenic 60.0%
GGCGCAGATCCGAGGAGTTT+GGG + chr2_1:73866712-73866731 Msa0224110:five_prime_UTR 60.0%
CAGCAGGCTCAGTCAGGAAC+TGG + chr2_1:73868354-73868373 Msa0224110:CDS 60.0%
TTTGGCCCCAACAAGGGAGC+TGG + chr2_1:73868449-73868468 Msa0224110:CDS 60.0%
CTGTGCCAGCTCCCTTGTTG+GGG - chr2_1:73868457-73868476 None:intergenic 60.0%
GCTGTGCCAGCTCCCTTGTT+GGG - chr2_1:73868458-73868477 None:intergenic 60.0%
TGCTGTGCCAGCTCCCTTGT+TGG - chr2_1:73868459-73868478 None:intergenic 60.0%
GTGATCTCCAGAGCCTCTGG+AGG - chr2_1:73869035-73869054 None:intergenic 60.0%
ACACAAGGCGCAAGGTGGAC+TGG + chr2_1:73869222-73869241 Msa0224110:CDS 60.0%
CTCAGCCACCCATGGAGACA+TGG + chr2_1:73869280-73869299 Msa0224110:CDS 60.0%
! ACCACTGACCTTTTGGCCCG+TGG + chr2_1:73869365-73869384 Msa0224110:CDS 60.0%
ACCACGGGCCAAAAGGTCAG+TGG - chr2_1:73869369-73869388 None:intergenic 60.0%
GCCCACTTCGCCCAATACGA+TGG - chr2_1:73869457-73869476 None:intergenic 60.0%
AACTCCACGGCGCAGATCCG+AGG + chr2_1:73866704-73866723 Msa0224110:five_prime_UTR 65.0%
AACTCCTCGGATCTGCGCCG+TGG - chr2_1:73866711-73866730 None:intergenic 65.0%
CGGCGCAGATCCGAGGAGTT+TGG + chr2_1:73866711-73866730 Msa0224110:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 73866615 73869905 73866615 ID=Msa0224110;Name=Msa0224110
chr2_1 mRNA 73866615 73869905 73866615 ID=Msa0224110-mRNA-1;Parent=Msa0224110;Name=Msa0224110-mRNA-1;_AED=0.06;_eAED=0.06;_QI=196|1|1|1|0.75|0.6|5|277|421
chr2_1 exon 73866615 73866808 73866615 ID=Msa0224110-mRNA-1:exon:21727;Parent=Msa0224110-mRNA-1
chr2_1 exon 73867470 73867539 73867470 ID=Msa0224110-mRNA-1:exon:21728;Parent=Msa0224110-mRNA-1
chr2_1 exon 73867619 73867725 73867619 ID=Msa0224110-mRNA-1:exon:21729;Parent=Msa0224110-mRNA-1
chr2_1 exon 73868286 73868716 73868286 ID=Msa0224110-mRNA-1:exon:21730;Parent=Msa0224110-mRNA-1
chr2_1 exon 73868969 73869905 73868969 ID=Msa0224110-mRNA-1:exon:21731;Parent=Msa0224110-mRNA-1
chr2_1 five_prime_UTR 73866615 73866808 73866615 ID=Msa0224110-mRNA-1:five_prime_utr;Parent=Msa0224110-mRNA-1
chr2_1 five_prime_UTR 73867470 73867471 73867470 ID=Msa0224110-mRNA-1:five_prime_utr;Parent=Msa0224110-mRNA-1
chr2_1 CDS 73867472 73867539 73867472 ID=Msa0224110-mRNA-1:cds;Parent=Msa0224110-mRNA-1
chr2_1 CDS 73867619 73867725 73867619 ID=Msa0224110-mRNA-1:cds;Parent=Msa0224110-mRNA-1
chr2_1 CDS 73868286 73868716 73868286 ID=Msa0224110-mRNA-1:cds;Parent=Msa0224110-mRNA-1
chr2_1 CDS 73868969 73869628 73868969 ID=Msa0224110-mRNA-1:cds;Parent=Msa0224110-mRNA-1
chr2_1 three_prime_UTR 73869629 73869905 73869629 ID=Msa0224110-mRNA-1:three_prime_utr;Parent=Msa0224110-mRNA-1
Gene Sequence

>Msa0224110

ATGGCAGGTTTAGCTCCGGAAGGTTCACAATTTGATACCAAGCAGTTTGACACTAAGATGAATGAATTGCTTTCCACTGAAGGACAAGAATTCTTCACCTCATACGATGAAGTTCATGAAACTTTTGATGCAATGGGATTGATAGAAAATCTTCTGAGAGGCATATATGCTTATGGTTTTGAGAAGCCATCTGCAATACAGCAAAGGGGAATTGTTCCTTTTATCAAGGGTCTCGATGTGATTCAGCAGGCTCAGTCAGGAACTGGAAAGACAGCGACGTTCTGTTCTGGAATTTTGCAGCAGCTTGATTATGGTTTGGTTCAGTGCCAGGCTTTGGTTTTGGCCCCAACAAGGGAGCTGGCACAGCAGATTGAGAAGGTTATGAGAGCACTTGGTGATTACCTTGGTGTTAAGGTTCATGCTTGTGTTGGTGGCACTAGTGTTCGTGAGGATCAGCGTATTCTCCAAACTGGTGTCCATGTTGTTGTTGGTACTCCTGGACGTGTGTTTGACATGCTGCGAAGACAGTCTCTTCGGTCAGATTGCATTAAAATGTTTGTTTTGGATGAAGCTGATGAAATGCTTTCACGTGGTTTCAAGGACCAGATCTACGACATCTTCCAGTTGCTGCCACCCAAAATTCAGGTTGGAGTGTTCTCTGCTACAATGCCTCCAGAGGCTCTGGAGATCACAAGGAAGTTCATGAATAAGCCAGTTAGAATTCTGGTAAAGCGTGATGAATTGACCCTTGAGGGTATCAAGCAGTTCTACGTGAATGTTGACAAGGAAGAGTGGAAACTAGAGACATTGTGTGATCTTTATGAGACTCTGGCCATCACTCAGAGTGTCATTTTTGTGAACACAAGGCGCAAGGTGGACTGGCTCACTGACAAGATGCGAGGCAGAGACCATACTGTCTCAGCCACCCATGGAGACATGGACCAAAACACTCGTGATATCATCATGCGTGAATTCCGCTCTGGCTCTTCTCGAGTTCTCATTACCACTGACCTTTTGGCCCGTGGTATTGATGTTCAGCAAGTGTCTCTGGTCATAAATTATGATTTGCCTACCCAACCTGAAAACTATCTCCATCGTATTGGGCGAAGTGGGCGTTTTGGAAGAAAAGGTGTTGCTATCAACTTCGTCACATTGGAAGATGCAAGAATGCTGGCTGATATTCAGAAGTTCTATAATGTGATCGTAGAGGAATGCCTTCAAATGTTGCTGATCTACTCTGATGAGTTAATTTTTGTTCTTTGTTAA
Protein sequence

>Msa0224110

MAGLAPEGSQFDTKQFDTKMNELLSTEGQEFFTSYDEVHETFDAMGLIENLLRGIYAYGFEKPSAIQQRGIVPFIKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQTGVHVVVGTPGRVFDMLRRQSLRSDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRGRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTLEDARMLADIQKFYNVIVEECLQMLLIYSDELIFVLC*