AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0556430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556430 MtrunA17_Chr5g0393501 47.264 201 106 0 5 205 41 241 3.76e-60 189
Msa0556430 MtrunA17_Chr4g0034781 26.178 191 134 2 5 193 18 203 5.93e-12 62.8
Msa0556430 MtrunA17_Chr4g0044671 25.654 191 135 2 5 193 24 209 1.65e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCTAATATTGATGAATT+TGG 0.071532 4_1:+54635991 Msa0556430:CDS
TATAAGGCATTGTTAAAATT+TGG 0.194239 4_1:-54635780 None:intergenic
AAGAATTCCGCAATGAAAAT+TGG 0.202897 4_1:-54635691 None:intergenic
TGATGAATTTGGGACAAAAT+TGG 0.234698 4_1:+54636002 Msa0556430:CDS
TACTATGAAATCAATGATTA+TGG 0.240311 4_1:+54635832 Msa0556430:CDS
CATCTTCCTTTCATTCATAA+TGG 0.274505 4_1:+54635528 Msa0556430:five_prime_UTR
GTCCCAAATTCATCAATATT+AGG 0.280924 4_1:-54635994 None:intergenic
GTTTAAGTGTGACTCAATTA+TGG 0.303074 4_1:+54636306 Msa0556430:CDS
TGCCTAATATTGATGAATTT+GGG 0.308974 4_1:+54635992 Msa0556430:CDS
CTAAATATGTAAAAGATATT+AGG 0.309400 4_1:-54635855 None:intergenic
ACCGTGGAAATTCGTTTGTT+TGG 0.318850 4_1:-54635918 None:intergenic
TCCCAACAAAAGGTAATATA+AGG 0.319342 4_1:-54635796 None:intergenic
TTGCATACAGTGATGAAATA+AGG 0.320941 4_1:-54635562 None:intergenic
GGAAATTCGTTTGTTTGGAT+TGG 0.329638 4_1:-54635913 None:intergenic
GAAATTCGTTTGTTTGGATT+GGG 0.339538 4_1:-54635912 None:intergenic
TGCGGAATTCTTCAATGAAC+TGG 0.345003 4_1:+54635702 Msa0556430:CDS
TTCCACATACACGTGTTTCA+TGG 0.348799 4_1:+54635889 Msa0556430:CDS
TTAAACTCTAAAATATCATT+TGG 0.369978 4_1:-54636289 None:intergenic
CAGATCTTACCAAATGACTT+TGG 0.384038 4_1:+54636103 Msa0556430:intron
TGCGAGTTATAGATGTAGTT+TGG 0.385150 4_1:-54635948 None:intergenic
CAATGAACTGGATGAACAAT+GGG 0.420513 4_1:+54635714 Msa0556430:CDS
ACACAAACTATTGTTAAGAT+AGG 0.424067 4_1:+54636226 Msa0556430:CDS
AATATTACAATACTTAAAAC+AGG 0.460074 4_1:-54636375 None:intergenic
TCCCAATGATTCCCAACAAA+AGG 0.467567 4_1:-54635806 None:intergenic
CATTCTCATTTACGATTGTG+TGG 0.506907 4_1:+54636148 Msa0556430:CDS
AGTGATGAAATAAGGTCTGT+TGG 0.517513 4_1:-54635554 None:intergenic
CATTATGAATGAAAGGAAGA+TGG 0.523264 4_1:-54635527 None:intergenic
TTGTTAAGATAGGACGTAGC+TGG 0.529092 4_1:+54636236 Msa0556430:CDS
GGTTAAATACAACAATGTGT+TGG 0.550805 4_1:-54635759 None:intergenic
TCAATGAACTGGATGAACAA+TGG 0.558244 4_1:+54635713 Msa0556430:CDS
GGCCATGAAACACGTGTATG+TGG 0.560867 4_1:-54635891 None:intergenic
TGGAGTCCATTATGAATGAA+AGG 0.569698 4_1:-54635534 None:intergenic
GGAATGGTGATAATTAGTAA+TGG 0.583941 4_1:-54636435 None:intergenic
TCCAAACAAACGAATTTCCA+CGG 0.596587 4_1:+54635917 Msa0556430:CDS
GTAGTTTGGCTATGATACCG+TGG 0.697972 4_1:-54635934 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTATTTTTTAGCATAA+AGG - chr4_1:54636266-54636285 None:intergenic 10.0%
!!! AATATTATTTTTTAGCATAA+AGG - chr4_1:54636266-54636285 None:intergenic 10.0%
!! CTAAATATGTAAAAGATATT+AGG - chr4_1:54635858-54635877 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG + chr4_1:54635856-54635875 Msa0556430:CDS 15.0%
!!! TTAAACTCTAAAATATCATT+TGG - chr4_1:54636292-54636311 None:intergenic 15.0%
!! AATATTACAATACTTAAAAC+AGG - chr4_1:54636378-54636397 None:intergenic 15.0%
!! CTAAATATGTAAAAGATATT+AGG - chr4_1:54635858-54635877 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG + chr4_1:54635856-54635875 Msa0556430:CDS 15.0%
!!! TTAAACTCTAAAATATCATT+TGG - chr4_1:54636292-54636311 None:intergenic 15.0%
!! AATATTACAATACTTAAAAC+AGG - chr4_1:54636378-54636397 None:intergenic 15.0%
!!! TATAAGGCATTGTTAAAATT+TGG - chr4_1:54635783-54635802 None:intergenic 20.0%
!! TACTATGAAATCAATGATTA+TGG + chr4_1:54635832-54635851 Msa0556430:CDS 20.0%
!!! TATAAGGCATTGTTAAAATT+TGG - chr4_1:54635783-54635802 None:intergenic 20.0%
!! TACTATGAAATCAATGATTA+TGG + chr4_1:54635832-54635851 Msa0556430:CDS 20.0%
! ATGCCTAATATTGATGAATT+TGG + chr4_1:54635991-54636010 Msa0556430:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG + chr4_1:54635992-54636011 Msa0556430:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG + chr4_1:54636034-54636053 Msa0556430:intron 25.0%
! AAAAAAGATCCAAAGTCATT+TGG - chr4_1:54636115-54636134 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG + chr4_1:54636226-54636245 Msa0556430:CDS 25.0%
! ATGCCTAATATTGATGAATT+TGG + chr4_1:54635991-54636010 Msa0556430:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG + chr4_1:54635992-54636011 Msa0556430:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG + chr4_1:54636034-54636053 Msa0556430:intron 25.0%
! AAAAAAGATCCAAAGTCATT+TGG - chr4_1:54636115-54636134 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG + chr4_1:54636226-54636245 Msa0556430:CDS 25.0%
CATTATGAATGAAAGGAAGA+TGG - chr4_1:54635530-54635549 None:intergenic 30.0%
CATCTTCCTTTCATTCATAA+TGG + chr4_1:54635528-54635547 Msa0556430:five_prime_UTR 30.0%
TTGCATACAGTGATGAAATA+AGG - chr4_1:54635565-54635584 None:intergenic 30.0%
TCACTGTATGCAAAAAAATC+AGG + chr4_1:54635572-54635591 Msa0556430:exon 30.0%
AAGAATTCCGCAATGAAAAT+TGG - chr4_1:54635694-54635713 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG - chr4_1:54635762-54635781 None:intergenic 30.0%
! TGCCTTATATTACCTTTTGT+TGG + chr4_1:54635794-54635813 Msa0556430:CDS 30.0%
! GCCTTATATTACCTTTTGTT+GGG + chr4_1:54635795-54635814 Msa0556430:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG - chr4_1:54635799-54635818 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG - chr4_1:54635915-54635934 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG - chr4_1:54635975-54635994 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG - chr4_1:54635997-54636016 None:intergenic 30.0%
! TGATGAATTTGGGACAAAAT+TGG + chr4_1:54636002-54636021 Msa0556430:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG + chr4_1:54636118-54636137 Msa0556430:CDS 30.0%
GTTTAAGTGTGACTCAATTA+TGG + chr4_1:54636306-54636325 Msa0556430:CDS 30.0%
CATTATGAATGAAAGGAAGA+TGG - chr4_1:54635530-54635549 None:intergenic 30.0%
CATCTTCCTTTCATTCATAA+TGG + chr4_1:54635528-54635547 Msa0556430:five_prime_UTR 30.0%
TTGCATACAGTGATGAAATA+AGG - chr4_1:54635565-54635584 None:intergenic 30.0%
TCACTGTATGCAAAAAAATC+AGG + chr4_1:54635572-54635591 Msa0556430:exon 30.0%
AAGAATTCCGCAATGAAAAT+TGG - chr4_1:54635694-54635713 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG - chr4_1:54635762-54635781 None:intergenic 30.0%
! TGCCTTATATTACCTTTTGT+TGG + chr4_1:54635794-54635813 Msa0556430:CDS 30.0%
! GCCTTATATTACCTTTTGTT+GGG + chr4_1:54635795-54635814 Msa0556430:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG - chr4_1:54635799-54635818 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG - chr4_1:54635915-54635934 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG - chr4_1:54635975-54635994 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG - chr4_1:54635997-54636016 None:intergenic 30.0%
! TGATGAATTTGGGACAAAAT+TGG + chr4_1:54636002-54636021 Msa0556430:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG + chr4_1:54636118-54636137 Msa0556430:CDS 30.0%
GTTTAAGTGTGACTCAATTA+TGG + chr4_1:54636306-54636325 Msa0556430:CDS 30.0%
TGGAGTCCATTATGAATGAA+AGG - chr4_1:54635537-54635556 None:intergenic 35.0%
AGTGATGAAATAAGGTCTGT+TGG - chr4_1:54635557-54635576 None:intergenic 35.0%
! GCTTGATCCAATTTTCATTG+CGG + chr4_1:54635684-54635703 Msa0556430:CDS 35.0%
TCAATGAACTGGATGAACAA+TGG + chr4_1:54635713-54635732 Msa0556430:CDS 35.0%
CAATGAACTGGATGAACAAT+GGG + chr4_1:54635714-54635733 Msa0556430:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG + chr4_1:54635804-54635823 Msa0556430:CDS 35.0%
! ACCTTTTGTTGGGAATCATT+GGG + chr4_1:54635805-54635824 Msa0556430:CDS 35.0%
GGAAATTCGTTTGTTTGGAT+TGG - chr4_1:54635916-54635935 None:intergenic 35.0%
TCCAAACAAACGAATTTCCA+CGG + chr4_1:54635917-54635936 Msa0556430:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG - chr4_1:54635951-54635970 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG - chr4_1:54635976-54635995 None:intergenic 35.0%
GATCTGCAAGAAGGAAAAAA+TGG - chr4_1:54636089-54636108 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG - chr4_1:54636098-54636117 None:intergenic 35.0%
CAGATCTTACCAAATGACTT+TGG + chr4_1:54636103-54636122 Msa0556430:intron 35.0%
CATTCTCATTTACGATTGTG+TGG + chr4_1:54636148-54636167 Msa0556430:CDS 35.0%
TGGAGTCCATTATGAATGAA+AGG - chr4_1:54635537-54635556 None:intergenic 35.0%
AGTGATGAAATAAGGTCTGT+TGG - chr4_1:54635557-54635576 None:intergenic 35.0%
! GCTTGATCCAATTTTCATTG+CGG + chr4_1:54635684-54635703 Msa0556430:CDS 35.0%
TCAATGAACTGGATGAACAA+TGG + chr4_1:54635713-54635732 Msa0556430:CDS 35.0%
CAATGAACTGGATGAACAAT+GGG + chr4_1:54635714-54635733 Msa0556430:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG + chr4_1:54635804-54635823 Msa0556430:CDS 35.0%
! ACCTTTTGTTGGGAATCATT+GGG + chr4_1:54635805-54635824 Msa0556430:CDS 35.0%
GGAAATTCGTTTGTTTGGAT+TGG - chr4_1:54635916-54635935 None:intergenic 35.0%
TCCAAACAAACGAATTTCCA+CGG + chr4_1:54635917-54635936 Msa0556430:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG - chr4_1:54635951-54635970 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG - chr4_1:54635976-54635995 None:intergenic 35.0%
GATCTGCAAGAAGGAAAAAA+TGG - chr4_1:54636089-54636108 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG - chr4_1:54636098-54636117 None:intergenic 35.0%
CAGATCTTACCAAATGACTT+TGG + chr4_1:54636103-54636122 Msa0556430:intron 35.0%
CATTCTCATTTACGATTGTG+TGG + chr4_1:54636148-54636167 Msa0556430:CDS 35.0%
TGCGGAATTCTTCAATGAAC+TGG + chr4_1:54635702-54635721 Msa0556430:CDS 40.0%
TCCCAATGATTCCCAACAAA+AGG - chr4_1:54635809-54635828 None:intergenic 40.0%
TTCCACATACACGTGTTTCA+TGG + chr4_1:54635889-54635908 Msa0556430:CDS 40.0%
ACCGTGGAAATTCGTTTGTT+TGG - chr4_1:54635921-54635940 None:intergenic 40.0%
TTGTTAAGATAGGACGTAGC+TGG + chr4_1:54636236-54636255 Msa0556430:CDS 40.0%
TGCGGAATTCTTCAATGAAC+TGG + chr4_1:54635702-54635721 Msa0556430:CDS 40.0%
TCCCAATGATTCCCAACAAA+AGG - chr4_1:54635809-54635828 None:intergenic 40.0%
TTCCACATACACGTGTTTCA+TGG + chr4_1:54635889-54635908 Msa0556430:CDS 40.0%
ACCGTGGAAATTCGTTTGTT+TGG - chr4_1:54635921-54635940 None:intergenic 40.0%
TTGTTAAGATAGGACGTAGC+TGG + chr4_1:54636236-54636255 Msa0556430:CDS 40.0%
GTAGTTTGGCTATGATACCG+TGG - chr4_1:54635937-54635956 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG + chr4_1:54635963-54635982 Msa0556430:CDS 45.0%
GTAGTTTGGCTATGATACCG+TGG - chr4_1:54635937-54635956 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG + chr4_1:54635963-54635982 Msa0556430:CDS 45.0%
GATCAAGCTCAGCACTCATC+TGG - chr4_1:54635672-54635691 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG - chr4_1:54635894-54635913 None:intergenic 50.0%
GATCAAGCTCAGCACTCATC+TGG - chr4_1:54635672-54635691 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG - chr4_1:54635894-54635913 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 54635510 54636443 54635510 ID=Msa0556430;Name=Msa0556430
chr4_1 mRNA 54635510 54636443 54635510 ID=Msa0556430-mRNA-1;Parent=Msa0556430;Name=Msa0556430-mRNA-1;_AED=0.05;_eAED=0.05;_QI=69|1|1|1|0|0|3|59|214
chr4_1 exon 54635510 54635593 54635510 ID=Msa0556430-mRNA-1:exon:16088;Parent=Msa0556430-mRNA-1
chr4_1 exon 54635673 54636023 54635673 ID=Msa0556430-mRNA-1:exon:16089;Parent=Msa0556430-mRNA-1
chr4_1 exon 54636106 54636443 54636106 ID=Msa0556430-mRNA-1:exon:16090;Parent=Msa0556430-mRNA-1
chr4_1 five_prime_UTR 54635510 54635578 54635510 ID=Msa0556430-mRNA-1:five_prime_utr;Parent=Msa0556430-mRNA-1
chr4_1 CDS 54635579 54635593 54635579 ID=Msa0556430-mRNA-1:cds;Parent=Msa0556430-mRNA-1
chr4_1 CDS 54635673 54636023 54635673 ID=Msa0556430-mRNA-1:cds;Parent=Msa0556430-mRNA-1
chr4_1 CDS 54636106 54636384 54636106 ID=Msa0556430-mRNA-1:cds;Parent=Msa0556430-mRNA-1
chr4_1 three_prime_UTR 54636385 54636443 54636385 ID=Msa0556430-mRNA-1:three_prime_utr;Parent=Msa0556430-mRNA-1
Gene Sequence

>Msa0556430

ATGCAAAAAAATCAGATGAGTGCTGAGCTTGATCCAATTTTCATTGCGGAATTCTTCAATGAACTGGATGAACAATGGGTCTTGATTGATGAAAATGAGAACCAACACATTGTTGTATTTAACCAAATTTTAACAATGCCTTATATTACCTTTTGTTGGGAATCATTGGGAATTTACTATGAAATCAATGATTATGGCCTAATATCTTTTACATATTTAGGTGAAAGTAAGTTCCACATACACGTGTTTCATGGCCCAATCCAAACAAACGAATTTCCACGGTATCATAGCCAAACTACATCTATAACTCGCAACCCAACTTTTGTGGTTTTTATGCCTAATATTGATGAATTTGGGACAAAATTGATCTTACCAAATGACTTTGGATCTTTTTTGAAAGGAAAAAAACATTCTCATTTACGATTGTGTGGATCGTCAAATTCTATCACAACTTGCAAGTTGTACTTTGAAGATAATCAACAACAAACACAAACTATTGTTAAGATAGGACGTAGCTGGACTACCTTTATGCTAAAAAATAATATTTATCCAAATGATATTTTAGAGTTTAAGTGTGACTCAATTATGGCAACAAATATTGTCATTGTAATCAAGTATGATGAAAGTTCAAGACACCTGTTTTAA

Protein sequence

>Msa0556430

MQKNQMSAELDPIFIAEFFNELDEQWVLIDENENQHIVVFNQILTMPYITFCWESLGIYYEINDYGLISFTYLGESKFHIHVFHGPIQTNEFPRYHSQTTSITRNPTFVVFMPNIDEFGTKLILPNDFGSFLKGKKHSHLRLCGSSNSITTCKLYFEDNQQQTQTIVKIGRSWTTFMLKNNIYPNDILEFKCDSIMATNIVIVIKYDESSRHLF*