AlfalfaGEDB | Msa0653020

M. sativa cultivar zhongmu-4 / Msa0653020

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0653020	RHN62398.1	97.222	144	3	1	1	143	11	154	9.95e-96	283

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0653020	sp\|Q38741\|SBP1_ANTMA	57.554	139	49	2	1	139	1	129	5.53e-47	151

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0653020	A0A396IC84	97.222	144	3	1	1	143	11	154	4.75e-96	283

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TFs/TRs:

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Gene ID	Type	Classification
Msa0653020	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0653020	0.804542	2.054904e-49	-8.615850e-47
Msa0022640	Msa0653020	0.814381	1.585622e-51	-8.615850e-47
Msa0044100	Msa0653020	0.821343	4.247512e-53	-8.615850e-47
Msa0124110	Msa0653020	0.812122	4.969383e-51	-8.615850e-47
Msa0157290	Msa0653020	0.803879	2.824221e-49	-8.615850e-47
Msa0161680	Msa0653020	0.813281	2.771666e-51	-8.615850e-47
Msa0165430	Msa0653020	0.808046	3.753198e-50	-8.615850e-47
Msa0173990	Msa0653020	0.811419	7.069024e-51	-8.615850e-47
Msa0183750	Msa0653020	0.820992	5.115252e-53	-8.615850e-47
Msa0185640	Msa0653020	-0.824360	8.418469e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0653020	MtrunA17_Chr4g0046991	97.222	144	3	1	1	143	11	154	9.15e-100	283
Msa0653020	MtrunA17_Chr2g0282521	72.477	109	27	2	33	140	36	142	1.26e-49	155
Msa0653020	MtrunA17_Chr8g0360361	72.941	85	23	0	59	143	62	146	5.80e-43	140
Msa0653020	MtrunA17_Chr8g0334671	61.607	112	36	2	27	136	155	261	5.00e-38	131
Msa0653020	MtrunA17_Chr7g0231911	74.074	81	21	0	62	142	149	229	9.22e-38	130
Msa0653020	MtrunA17_Chr7g0258471	74.026	77	20	0	59	135	139	215	1.01e-36	129
Msa0653020	MtrunA17_Chr5g0420051	60.000	95	35	1	50	141	166	260	1.27e-35	127
Msa0653020	MtrunA17_Chr3g0122411	69.737	76	23	0	60	135	163	238	3.76e-34	123
Msa0653020	MtrunA17_Chr2g0302791	59.794	97	35	1	47	139	120	216	4.80e-34	125
Msa0653020	MtrunA17_Chr4g0061651	72.973	74	20	0	62	135	123	196	1.27e-33	122

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0653020	AT2G33810.1	60.284	141	44	3	1	139	1	131	2.31e-44	142
Msa0653020	AT1G53160.3	67.391	92	27	1	46	137	41	129	4.23e-38	127
Msa0653020	AT1G53160.2	67.391	92	27	1	46	137	41	129	4.27e-38	127
Msa0653020	AT1G53160.1	67.391	92	27	1	46	137	41	129	4.27e-38	127
Msa0653020	AT3G15270.1	69.512	82	25	0	62	143	63	144	9.08e-37	124
Msa0653020	AT1G69170.1	64.894	94	30	1	50	140	109	202	4.23e-36	128
Msa0653020	AT3G60030.1	62.500	96	36	0	47	142	112	207	4.43e-36	131
Msa0653020	AT1G02065.1	64.286	98	33	1	40	135	164	261	8.34e-36	126
Msa0653020	AT2G47070.1	72.368	76	21	0	61	136	105	180	2.53e-34	126
Msa0653020	AT1G27360.2	56.701	97	42	0	39	135	152	248	9.19e-33	119

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Find 51 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGCAATTGACTATCA+AGG	0.509871	4_3:-60824123	Msa0653020:CDS
AAACTCAAACTATTCATATG+AGG	0.504948	4_3:+60828354	None:intergenic
AAGAAGTTGCAGAAGGCGTT+TGG	0.399194	4_3:-60824169	Msa0653020:CDS
AAGAAGTTTGAGGTACAAAG+AGG	0.636165	4_3:-60828243	Msa0653020:CDS
AAGAGGAAGAGGATGGTAGA+AGG	0.346008	4_3:-60828181	Msa0653020:CDS
AAGAGGATGGTAGAAGGAAG+AGG	0.452598	4_3:-60828175	Msa0653020:CDS
AATCCTGCAATGTGTACGGT+AGG	0.674434	4_3:+60827999	None:intergenic
AATGTGTACGGTAGGTGCTT+TGG	0.528660	4_3:+60828007	None:intergenic
ACATTGCAGGATTGCAGCAA+AGG	0.514685	4_3:-60827989	Msa0653020:CDS
ACCTTGTTTCTAGATCTCTC+TGG	0.509860	4_3:-60828309	Msa0653020:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGTGTGAGAGAATGTATT+AGG	+	chr4_3:60823845-60823864	None:intergenic	30.0%
AGGTTCTCTCAAAAAAAAAG+TGG	+	chr4_3:60823961-60823980	None:intergenic	30.0%
CTCCATAATTTCAAGGAAAA+AGG	+	chr4_3:60824003-60824022	None:intergenic	30.0%
TTCTTGTCTCCATAATTTCA+AGG	+	chr4_3:60824010-60824029	None:intergenic	30.0%
TTGAAATTATGGAGACAAGA+AGG	-	chr4_3:60824009-60824028	Msa0653020:three_prime_UTR	30.0%
GATGTGAAATATTCTTGTTG+AGG	-	chr4_3:60824436-60824455	Msa0653020:intron	30.0%
GACAATGCATAAAATATGCA+AGG	+	chr4_3:60824617-60824636	None:intergenic	30.0%
GTTGATGTTGGCATAATAAA+AGG	+	chr4_3:60824679-60824698	None:intergenic	30.0%
AATAAGCAGAGGAAAAATCT+TGG	+	chr4_3:60824860-60824879	None:intergenic	30.0%
ACTTGAAACCCAAATTAAGT+AGG	+	chr4_3:60825027-60825046	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	60823780	60828528	60823780	ID=Msa0653020;Name=Msa0653020
chr4_3	mRNA	60823780	60828528	60823780	ID=Msa0653020-mRNA-1;Parent=Msa0653020;Name=Msa0653020-mRNA-1;_AED=0.01;_eAED=0.01;_QI=237\|1\|1\|1\|1\|1\|2\|336\|143
chr4_3	exon	60823780	60824224	60823780	ID=Msa0653020-mRNA-1:exon:16577;Parent=Msa0653020-mRNA-1
chr4_3	exon	60827969	60828528	60827969	ID=Msa0653020-mRNA-1:exon:16576;Parent=Msa0653020-mRNA-1
chr4_3	five_prime_UTR	60828292	60828528	60828292	ID=Msa0653020-mRNA-1:five_prime_utr;Parent=Msa0653020-mRNA-1
chr4_3	CDS	60827969	60828291	60827969	ID=Msa0653020-mRNA-1:cds;Parent=Msa0653020-mRNA-1
chr4_3	CDS	60824116	60824224	60824116	ID=Msa0653020-mRNA-1:cds;Parent=Msa0653020-mRNA-1
chr4_3	three_prime_UTR	60823780	60824115	60823780	ID=Msa0653020-mRNA-1:three_prime_utr;Parent=Msa0653020-mRNA-1

Gene Sequence

>Msa0653020

ATGGAGACAAGAAGGTCAGAGGGAAAAAGAAGTTTGAGGTACAAAGAGGATCATGAGGAGGAAGAAGAGGAAGAGGATACGGATTTTGAAGAGGAAGAGGATGGTAGAAGGAAGAGGGTAGTGACAGATCTCTATAGCAAGAGAAGTTCCAAAAAAGCTGGAAGCTCAAATATTCCACCTTGTTGTCAAGTGGAGAATTGTGATGCTGATCTTAGTGAAGCTAAGCAATATCACCGGAGACATAAGGTCTGTGAGTATCATGCCAAAGCACCTACCGTACACATTGCAGGATTGCAGCAAAGGTTTTGTCAGCAATGTAGCAGATTCCATGGACTATCTGAATTTGATGACTCAAAAAGAAGTTGCAGAAGGCGTTTGGCTGGGCATAATGAGAGGCGCCGCAAAAGTGCAATTGACTATCAAGGAGAATGA

Protein sequence

>Msa0653020

METRRSEGKRSLRYKEDHEEEEEEEDTDFEEEEDGRRKRVVTDLYSKRSSKKAGSSNIPPCCQVENCDADLSEAKQYHRRHKVCEYHAKAPTVHIAGLQQRFCQQCSRFHGLSEFDDSKRSCRRRLAGHNERRRKSAIDYQGE*