AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0723210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723210 MtrunA17_Chr5g0414051 94.919 433 22 0 1 433 1 433 0.0 816
Msa0723210 MtrunA17_Chr5g0414091 67.464 418 116 6 24 424 21 435 0.0 555
Msa0723210 MtrunA17_Chr8g0391481 67.227 357 115 2 66 421 18 373 0.0 523
Msa0723210 MtrunA17_Chr5g0414061 60.369 434 145 7 6 424 21 442 0.0 518
Msa0723210 MtrunA17_Chr5g0414041 61.086 442 149 6 6 433 12 444 0.0 514
Msa0723210 MtrunA17_Chr3g0140371 67.507 357 113 3 66 422 9 362 0.0 511
Msa0723210 MtrunA17_Chr7g0226991 64.426 357 125 2 67 422 49 404 2.63e-175 496
Msa0723210 MtrunA17_Chr5g0414081 71.500 200 56 1 223 422 3 201 1.56e-107 316
Msa0723210 MtrunA17_Chr8g0387921 39.529 425 237 10 7 420 4 419 7.31e-99 301
Msa0723210 MtrunA17_Chr6g0456691 37.767 421 233 12 14 421 16 420 7.49e-94 289
Msa0723210 MtrunA17_Chr8g0347321 38.660 388 218 9 45 420 60 439 1.23e-93 289
Msa0723210 MtrunA17_Chr6g0456681 38.177 406 222 12 27 421 22 409 2.30e-85 267
Msa0723210 MtrunA17_Chr6g0451321 35.825 388 226 10 42 422 88 459 9.40e-81 256
Msa0723210 MtrunA17_Chr2g0283851 37.119 361 195 13 78 422 58 402 4.36e-75 240
Msa0723210 MtrunA17_Chr1g0188191 35.278 360 211 10 75 422 234 583 1.20e-67 226
Msa0723210 MtrunA17_Chr6g0476041 32.908 392 239 11 44 422 136 516 1.65e-65 218
Msa0723210 MtrunA17_Chr7g0259711 32.976 373 196 17 75 422 91 434 3.89e-52 181
Msa0723210 MtrunA17_Chr1g0175671 33.787 367 200 14 75 422 170 512 1.71e-50 178
Msa0723210 MtrunA17_Chr2g0287901 32.782 363 213 10 75 424 97 441 1.43e-49 174
Msa0723210 MtrunA17_Chr7g0258851 33.333 378 207 14 75 431 248 601 9.24e-49 175
Msa0723210 MtrunA17_Chr7g0221281 36.437 247 140 9 180 421 3 237 3.35e-47 163
Msa0723210 MtrunA17_Chr1g0213281 30.617 405 221 18 58 431 153 528 2.41e-45 164
Msa0723210 MtrunA17_Chr4g0071391 31.680 363 206 13 75 420 109 446 1.98e-44 160
Msa0723210 MtrunA17_Chr8g0384151 31.389 360 200 14 75 420 90 416 2.09e-44 160
Msa0723210 MtrunA17_Chr4g0006991 30.319 376 220 14 64 420 75 427 2.44e-44 160
Msa0723210 MtrunA17_Chr3g0105301 31.198 359 209 10 71 416 39 372 9.65e-43 154
Msa0723210 MtrunA17_Chr8g0368461 29.820 389 207 16 75 426 178 537 1.79e-41 154
Msa0723210 MtrunA17_Chr3g0126021 29.099 433 249 16 12 420 61 459 1.85e-41 153
Msa0723210 MtrunA17_Chr4g0070341 28.883 367 213 10 75 420 132 471 1.88e-41 153
Msa0723210 MtrunA17_Chr5g0430371 28.726 369 221 10 67 416 50 395 1.95e-41 151
Msa0723210 MtrunA17_Chr7g0269991 30.362 359 203 13 75 417 137 464 2.46e-41 152
Msa0723210 MtrunA17_Chr8g0360521 29.412 374 211 13 72 422 68 411 8.55e-41 150
Msa0723210 MtrunA17_Chr5g0413271 30.220 364 219 14 69 422 140 478 8.90e-41 151
Msa0723210 MtrunA17_Chr4g0068111 29.076 368 219 11 71 420 110 453 1.54e-39 147
Msa0723210 MtrunA17_Chr8g0384421 28.191 376 204 14 75 420 133 472 2.67e-39 147
Msa0723210 MtrunA17_Chr3g0113671 28.809 361 223 13 72 416 103 445 3.02e-39 146
Msa0723210 MtrunA17_Chr1g0171171 27.612 402 247 12 29 420 8 375 2.14e-38 142
Msa0723210 MtrunA17_Chr2g0325481 31.728 353 190 15 75 416 50 362 3.46e-38 142
Msa0723210 MtrunA17_Chr7g0242701 28.906 384 215 17 64 420 75 427 3.72e-38 143
Msa0723210 MtrunA17_Chr7g0221271 35.870 184 109 2 24 198 22 205 6.64e-38 136
Msa0723210 MtrunA17_Chr3g0097891 29.799 349 205 11 75 416 31 346 1.48e-36 137
Msa0723210 MtrunA17_Chr3g0085081 30.769 351 197 15 75 416 31 344 2.39e-36 136
Msa0723210 MtrunA17_Chr3g0091031 30.337 356 192 15 75 416 31 344 8.39e-36 135
Msa0723210 MtrunA17_Chr4g0005491 27.546 432 268 18 1 416 1 403 1.47e-35 135
Msa0723210 MtrunA17_Chr4g0041011 29.975 407 228 18 24 420 4 363 3.86e-35 133
Msa0723210 MtrunA17_Chr3g0085071 30.484 351 198 15 75 416 46 359 5.56e-35 133
Msa0723210 MtrunA17_Chr7g0243301 28.415 366 214 16 75 416 64 405 1.27e-34 133
Msa0723210 MtrunA17_Chr4g0045951 28.205 390 228 12 34 413 8 355 4.53e-33 127
Msa0723210 MtrunA17_Chr6g0455901 26.484 438 247 18 22 420 15 416 4.72e-33 129
Msa0723210 MtrunA17_Chr2g0314801 29.775 356 211 14 72 420 30 353 4.81e-33 127
Msa0723210 MtrunA17_Chr5g0413851 29.888 358 207 12 67 416 34 355 5.29e-33 127
Msa0723210 MtrunA17_Chr3g0114101 27.446 368 213 16 75 417 89 427 1.99e-32 127
Msa0723210 MtrunA17_Chr5g0437601 27.778 360 228 13 72 417 80 421 3.25e-32 127
Msa0723210 MtrunA17_Chr2g0314791 28.219 365 201 15 72 420 26 345 1.23e-31 123
Msa0723210 MtrunA17_Chr4g0045941 25.424 413 228 13 17 416 1 346 2.90e-31 122
Msa0723210 MtrunA17_Chr7g0221291 35.625 160 93 2 13 166 15 170 1.61e-30 115
Msa0723210 MtrunA17_Chr2g0283581 27.681 401 232 17 30 420 6 358 1.96e-30 120
Msa0723210 MtrunA17_Chr4g0005481 26.790 377 219 16 69 420 102 446 3.85e-30 121
Msa0723210 MtrunA17_Chr5g0437291 26.503 366 222 13 75 416 165 507 8.18e-30 121
Msa0723210 MtrunA17_Chr4g0049421 26.596 376 215 13 75 420 48 392 3.57e-27 112
Msa0723210 MtrunA17_Chr1g0178081 32.411 253 149 10 23 259 5 251 5.78e-25 105
Msa0723210 MtrunA17_Chr1g0178081 33.913 115 62 5 310 422 242 344 3.44e-12 67.4
Msa0723210 MtrunA17_Chr1g0210771 33.136 169 105 4 75 240 59 222 1.14e-24 104
Msa0723210 MtrunA17_Chr1g0209921 44.898 98 52 2 68 163 37 134 1.89e-21 93.6
Msa0723210 MtrunA17_Chr7g0275501 50.000 68 31 1 352 419 4 68 2.83e-16 73.2
Msa0723210 MtrunA17_Chr4g0057171 40.217 92 46 3 213 304 1 83 7.77e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723210 AT1G70230.1 47.596 416 185 10 23 423 17 414 3.94e-133 389
Msa0723210 AT4G23790.1 38.732 426 219 13 24 420 15 427 4.12e-95 293
Msa0723210 AT4G01080.1 39.440 393 220 9 32 418 48 428 9.55e-95 292
Msa0723210 AT1G01430.1 41.620 358 188 10 75 420 99 447 1.13e-93 290
Msa0723210 AT4G11090.1 38.389 422 235 11 17 420 11 425 8.27e-91 282
Msa0723210 AT5G15890.1 40.503 358 189 10 71 421 183 523 3.77e-89 280
Msa0723210 AT3G02440.2 38.997 359 201 8 75 426 135 482 1.12e-87 275
Msa0723210 AT3G02440.1 38.997 359 201 8 75 426 127 474 1.58e-87 275
Msa0723210 AT3G28150.1 35.809 377 214 10 60 422 50 412 1.81e-83 262
Msa0723210 AT5G15900.1 37.017 362 207 11 75 427 67 416 3.71e-80 254
Msa0723210 AT5G15900.2 37.017 362 207 11 75 427 86 435 5.53e-80 254
Msa0723210 AT4G25360.1 33.880 366 216 9 72 422 172 526 1.09e-63 214
Msa0723210 AT4G25360.2 33.880 366 216 9 72 422 172 526 1.09e-63 214
Msa0723210 AT5G51640.1 33.508 382 214 13 72 431 135 498 4.00e-62 209
Msa0723210 AT5G19160.1 29.336 467 275 13 3 431 6 455 1.51e-54 188
Msa0723210 AT5G06700.1 32.095 377 209 15 75 430 255 605 2.40e-48 174
Msa0723210 AT3G06080.2 31.608 367 210 12 75 422 103 447 2.92e-48 171
Msa0723210 AT5G64020.1 30.831 373 210 12 75 420 57 408 2.46e-47 167
Msa0723210 AT3G12060.1 31.316 380 214 15 75 433 196 549 5.76e-47 170
Msa0723210 AT3G11570.1 32.665 349 189 13 91 420 98 419 8.96e-47 167
Msa0723210 AT2G30010.1 30.457 394 240 12 41 420 23 396 3.48e-46 164
Msa0723210 AT2G30010.2 30.457 394 240 12 41 420 66 439 7.87e-46 164
Msa0723210 AT1G48880.1 33.795 361 192 15 70 417 110 436 2.09e-45 163
Msa0723210 AT2G14530.1 29.904 418 222 17 39 420 24 406 5.00e-43 156
Msa0723210 AT5G58600.1 30.252 357 216 10 75 420 66 400 5.51e-43 156
Msa0723210 AT5G06230.2 31.536 371 202 13 75 420 21 364 9.22e-43 154
Msa0723210 AT5G06230.1 31.536 371 202 13 75 420 62 405 2.22e-42 154
Msa0723210 AT3G62390.1 29.839 372 212 16 69 422 134 474 1.09e-41 154
Msa0723210 AT5G20680.6 28.198 383 207 11 69 420 112 457 1.64e-41 153
Msa0723210 AT5G20590.1 31.389 360 204 14 75 416 141 475 1.80e-41 154
Msa0723210 AT5G49340.1 27.869 366 220 10 75 420 100 441 2.15e-41 153
Msa0723210 AT5G20680.4 28.084 381 210 10 69 420 206 551 2.17e-41 154
Msa0723210 AT5G20680.1 28.084 381 210 10 69 420 206 551 2.17e-41 154
Msa0723210 AT5G20680.3 28.084 381 210 10 69 420 206 551 2.17e-41 154
Msa0723210 AT5G20680.5 28.084 381 210 10 69 420 206 551 2.17e-41 154
Msa0723210 AT5G20680.2 28.084 381 210 10 69 420 188 533 2.34e-41 154
Msa0723210 AT2G40160.2 28.837 430 252 19 19 416 12 419 2.74e-41 152
Msa0723210 AT2G40160.1 28.837 430 252 19 19 416 12 419 2.83e-41 152
Msa0723210 AT1G60790.1 27.249 389 239 14 54 421 169 534 2.29e-39 149
Msa0723210 AT1G73140.1 28.877 374 216 15 64 416 57 401 3.79e-39 145
Msa0723210 AT2G42570.1 30.518 367 201 15 67 420 39 364 4.53e-39 144
Msa0723210 AT2G40150.1 29.006 362 221 14 75 416 71 416 8.17e-39 145
Msa0723210 AT2G37720.1 28.982 383 202 11 70 420 138 482 1.28e-38 145
Msa0723210 AT3G55990.1 29.570 372 226 16 63 416 126 479 1.57e-38 145
Msa0723210 AT5G01360.2 28.184 369 234 13 63 417 24 375 2.85e-38 142
Msa0723210 AT5G01360.1 28.184 369 234 13 63 417 80 431 9.99e-38 142
Msa0723210 AT5G19160.2 29.706 340 196 10 3 309 6 335 2.93e-37 139
Msa0723210 AT2G38320.1 28.954 373 235 15 59 416 43 400 1.73e-36 138
Msa0723210 AT2G31110.2 29.428 367 201 16 65 416 35 358 2.88e-36 137
Msa0723210 AT2G30900.1 28.895 353 206 13 75 416 43 361 3.36e-35 134
Msa0723210 AT3G11030.1 28.255 361 226 14 75 420 106 448 1.08e-34 134
Msa0723210 AT2G40320.1 27.322 366 222 14 72 417 78 419 7.55e-34 131
Msa0723210 AT3G14850.2 30.641 359 198 15 75 420 34 354 7.59e-34 130
Msa0723210 AT1G29050.1 27.989 368 217 15 63 420 49 378 4.77e-33 128
Msa0723210 AT5G64470.3 27.297 370 217 15 74 420 54 394 2.10e-32 127
Msa0723210 AT5G64470.2 26.862 376 217 16 74 420 54 400 2.89e-31 124
Msa0723210 AT3G54260.1 27.042 355 211 12 75 416 51 370 3.56e-31 123
Msa0723210 AT1G78710.1 29.700 367 210 15 64 420 28 356 6.06e-31 122
Msa0723210 AT3G06080.1 31.325 249 145 6 75 309 103 339 6.30e-31 122
Msa0723210 AT2G40160.3 28.758 306 197 11 19 309 12 311 9.74e-31 120
Msa0723210 AT2G34070.1 26.975 367 215 15 67 420 57 383 4.03e-30 120
Msa0723210 AT3G62390.2 29.572 257 151 8 67 309 132 372 8.76e-30 120
Msa0723210 AT2G38320.2 28.834 326 203 14 105 416 7 317 8.99e-29 115
Msa0723210 AT5G01620.2 26.776 366 230 11 71 420 106 449 1.82e-28 117
Msa0723210 AT5G01620.1 26.776 366 230 11 71 420 106 449 1.82e-28 117
Msa0723210 AT5G01620.3 26.776 366 230 11 71 420 114 457 2.53e-28 116
Msa0723210 AT5G58600.2 32.353 238 143 7 75 308 66 289 1.03e-27 112
Msa0723210 AT5G06230.3 31.621 253 145 7 75 309 62 304 1.52e-27 112
Msa0723210 AT5G20590.2 34.715 193 109 6 75 251 141 332 1.74e-27 113
Msa0723210 AT2G30900.2 36.628 172 92 6 75 239 43 204 1.01e-23 100
Msa0723210 AT2G31110.3 31.053 190 112 5 65 246 35 213 1.75e-23 99.8
Msa0723210 AT1G78710.2 35.676 185 107 6 51 226 3 184 5.52e-22 96.3
Msa0723210 AT5G64470.1 26.389 288 171 12 74 338 54 323 8.94e-20 90.1
Msa0723210 AT5G64470.4 26.882 279 163 12 74 329 54 314 6.53e-19 87.4

Find 138 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATTTCTTGATGATTT+TGG 0.053119 5_1:-20805964 None:intergenic
GTCCTTAAAGCCTTTCTTAA+TGG 0.091385 5_1:-20808093 None:intergenic
TTCCATTAAGAAAGGCTTTA+AGG 0.121836 5_1:+20808091 Msa0723210:CDS
ATGATATGAAAATTTATATT+TGG 0.185679 5_1:+20806131 Msa0723210:five_prime_UTR
GCCACCAATGTGAACCTTTA+TGG 0.250930 5_1:-20807792 None:intergenic
TGCTTAAATTGTTCCGGTTT+CGG 0.254274 5_1:+20808039 Msa0723210:CDS
CAAACTACCTTTACTATAGA+TGG 0.262429 5_1:+20807611 Msa0723210:CDS
CCTTTATGGTGGACCGGTTT+AGG 0.263456 5_1:-20807778 None:intergenic
CACCAACAAATGTGCCATTA+TGG 0.283857 5_1:-20808660 None:intergenic
GGTCTTAATTACACTGATAT+TGG 0.285156 5_1:+20808060 Msa0723210:CDS
GTGACTACTTCAATGGAAAA+TGG 0.286087 5_1:+20807497 Msa0723210:CDS
TCTCTTGTATCCTTAATTCA+TGG 0.290646 5_1:+20805795 Msa0723210:five_prime_UTR
AGTAAAAGATGAAGTGTATA+TGG 0.296963 5_1:-20806513 None:intergenic
GGAAAGGGTGAGAAGGAAAT+TGG 0.307402 5_1:+20808249 Msa0723210:CDS
ACACAAGGACCTAGGTTTCT+TGG 0.313154 5_1:-20806082 None:intergenic
TACAAAGAAGAGACTCAAAT+TGG 0.314351 5_1:-20807735 None:intergenic
TGTGTTCATTGGTGTTTGCC+TGG 0.324131 5_1:+20808471 Msa0723210:CDS
CTTGGAAGATTGCACTCATT+TGG 0.324777 5_1:-20807637 None:intergenic
AAAGAGAAATCCATGAATTA+AGG 0.326553 5_1:-20805805 None:intergenic
AAAGAATAAAGTACAATATA+TGG 0.327184 5_1:-20806204 None:intergenic
TCTAGACAAGGAAAATGTTT+GGG 0.327479 5_1:+20808709 Msa0723210:three_prime_UTR
TTCCATAATGGCACATTTGT+TGG 0.341005 5_1:+20808658 Msa0723210:three_prime_UTR
TTCTAGACAAGGAAAATGTT+TGG 0.354572 5_1:+20808708 Msa0723210:three_prime_UTR
GACTTGATTGTGTTATCGTT+TGG 0.361764 5_1:+20807961 Msa0723210:CDS
AAATGGATTCATGACAATAA+AGG 0.362706 5_1:+20807514 Msa0723210:CDS
TGTTCAAAGACTGAACCTTA+TGG 0.369816 5_1:+20808228 Msa0723210:CDS
TTAGGATGCTTAAATTGTTC+CGG 0.372597 5_1:+20808033 Msa0723210:CDS
CACAAGGACCTAGGTTTCTT+GGG 0.377912 5_1:-20806081 None:intergenic
TCTCTGATCTTCTTCCATAA+TGG 0.379762 5_1:+20808646 Msa0723210:three_prime_UTR
AGATGATGAAGAAGTGGGAT+TGG 0.380315 5_1:+20808526 Msa0723210:CDS
TCATCACCTTGCACAAGAAA+TGG 0.391236 5_1:-20807856 None:intergenic
CCTAAACCGGTCCACCATAA+AGG 0.397698 5_1:+20807778 Msa0723210:CDS
AGGGATTAGATTTGAGGTAA+TGG 0.398286 5_1:+20808347 Msa0723210:CDS
CCAAGATCATGTTGATTAAC+CGG 0.398828 5_1:+20806022 Msa0723210:five_prime_UTR
AAATGGAAAGTGAGATTAGA+AGG 0.401729 5_1:+20808274 Msa0723210:CDS
GTGTTGAGGAGGGAGGATTC+AGG 0.403757 5_1:-20805998 None:intergenic
CCTTCGGTTTATTATGAGAA+TGG 0.405122 5_1:+20808003 Msa0723210:CDS
AAAGTTTAATATCTACATAT+TGG 0.408158 5_1:-20808750 None:intergenic
TTGGTTTATTAATGTTCCTT+CGG 0.408464 5_1:+20807987 Msa0723210:CDS
TCCTTCTCACCCTTTCCATA+AGG 0.413581 5_1:-20808243 None:intergenic
ATCCTAAGTGCTTGGGATCA+TGG 0.424578 5_1:-20805913 None:intergenic
GGTTTATTATGAGAATGGTT+TGG 0.433915 5_1:+20808008 Msa0723210:CDS
TAGTAAAGGTAGTTTGAATC+AGG 0.434714 5_1:-20807604 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.437679 5_1:+20808460 Msa0723210:CDS
AACTGTTTCAAAACCTAAAC+CGG 0.442193 5_1:+20807765 Msa0723210:CDS
ATTTGGAGGGATTAGATTTG+AGG 0.443087 5_1:+20808341 Msa0723210:CDS
AGTGTAATTAAGACCGAAAC+CGG 0.443307 5_1:-20808052 None:intergenic
TTGTGTTATCGTTTGGCAAT+TGG 0.445306 5_1:+20807968 Msa0723210:CDS
GTTGCTAAAGGGAATGGAAT+TGG 0.445312 5_1:+20808144 Msa0723210:CDS
CATCCATGTGACTACTTCAA+TGG 0.447132 5_1:+20807490 Msa0723210:CDS
ACACCGTTAGCGAACGGAAA+AGG 0.447241 5_1:-20808426 None:intergenic
TGCACACTTATCTATATTTG+AGG 0.448253 5_1:-20806245 None:intergenic
AAATGTATTGTATTAAAACT+TGG 0.452232 5_1:-20807895 None:intergenic
TATAAATAAAGAAGAACAAT+GGG 0.454593 5_1:-20806552 None:intergenic
TGGAAGAAGGGTCTTAGAAA+GGG 0.455875 5_1:-20806493 None:intergenic
AAGGATTGAGATTGAAGAAG+TGG 0.457929 5_1:+20808293 Msa0723210:CDS
AATAAAGGTCCTTTATATAG+TGG 0.460409 5_1:+20807529 Msa0723210:CDS
GATGAAGTGTATATGGAAGA+AGG 0.462811 5_1:-20806506 None:intergenic
TAAGCTCCTGGATGACCATC+TGG 0.467024 5_1:-20808396 None:intergenic
GGTAATGGATGTAACTGAAT+TGG 0.467735 5_1:+20808362 Msa0723210:CDS
ATGTCTCACTCTTCACTCCT+TGG 0.471169 5_1:-20808558 None:intergenic
GATGAAGAAGTGGGATTGGA+AGG 0.471664 5_1:+20808530 Msa0723210:CDS
TTGAGAGGAAAGTTGCTAAA+GGG 0.472700 5_1:+20808133 Msa0723210:CDS
TGTTCATGATCCTAAGTGCT+TGG 0.474792 5_1:-20805921 None:intergenic
TTGAGACCAGATGGTCATCC+AGG 0.475196 5_1:+20808390 Msa0723210:CDS
ATGCAACATTATCCGTGTAT+TGG 0.475380 5_1:+20807830 Msa0723210:CDS
GGTGATGAAAGATCAAAATC+CGG 0.476546 5_1:+20807871 Msa0723210:CDS
TCTAATTTATGATTTAATTG+AGG 0.477409 5_1:-20806052 None:intergenic
ATCTCATTCCAAGTATCTAT+AGG 0.478611 5_1:-20808495 None:intergenic
GTGTTCATTGGTGTTTGCCT+GGG 0.480611 5_1:+20808472 Msa0723210:CDS
CTTAGTTGGAGAAAAGTGTC+AGG 0.481705 5_1:-20807667 None:intergenic
TGCCTGGGCCTATAGATACT+TGG 0.482132 5_1:+20808487 Msa0723210:CDS
CCGGTTAATCAACATGATCT+TGG 0.485376 5_1:-20806022 None:intergenic
CATGTTGTTCCACTATATAA+AGG 0.487924 5_1:-20807538 None:intergenic
GTTCATGATCCTAAGTGCTT+GGG 0.491232 5_1:-20805920 None:intergenic
ACCATAAAGGTTCACATTGG+TGG 0.496277 5_1:+20807791 Msa0723210:CDS
TCTAGAGATGATGAAGAAGT+GGG 0.498464 5_1:+20808521 Msa0723210:CDS
ATGGAAGAAGGGTCTTAGAA+AGG 0.500151 5_1:-20806494 None:intergenic
AAAGGATTCATATAAGCTCC+TGG 0.500215 5_1:-20808408 None:intergenic
TTGGTTTCCATCTATAGTAA+AGG 0.502522 5_1:-20807618 None:intergenic
TATTGTATTAAAACTTGGCC+CGG 0.503179 5_1:-20807890 None:intergenic
TGTGAACCTTTATGGTGGAC+CGG 0.509927 5_1:-20807784 None:intergenic
TTTGGGGTTTGAAAGCAACT+TGG 0.510275 5_1:+20808726 Msa0723210:three_prime_UTR
TAATAAATTAACAAGCTAAG+AGG 0.510694 5_1:-20808582 None:intergenic
TTCTAGAGATGATGAAGAAG+TGG 0.512711 5_1:+20808520 Msa0723210:CDS
AAGACTGAACCTTATGGAAA+GGG 0.515408 5_1:+20808234 Msa0723210:CDS
CATAATGGCACATTTGTTGG+TGG 0.515551 5_1:+20808661 Msa0723210:three_prime_UTR
AGGAAAGTTGCTAAAGGGAA+TGG 0.518826 5_1:+20808138 Msa0723210:CDS
TGGTCGCCATTTCTTGTGCA+AGG 0.526450 5_1:+20807850 Msa0723210:CDS
AGCAGAAAACAAAGTAGGAA+AGG 0.526927 5_1:+20806416 Msa0723210:five_prime_UTR
ATGAAGTGTATATGGAAGAA+GGG 0.527722 5_1:-20806505 None:intergenic
ATTGAGAGGAAAGTTGCTAA+AGG 0.529202 5_1:+20808132 Msa0723210:CDS
AAAGTGTCAGGTTCAAACCT+TGG 0.538655 5_1:-20807655 None:intergenic
CAAATGAGTGCAATCTTCCA+AGG 0.538693 5_1:+20807638 Msa0723210:CDS
GTGATGAAAGATCAAAATCC+GGG 0.544715 5_1:+20807872 Msa0723210:CDS
AAAGACTGAACCTTATGGAA+AGG 0.548583 5_1:+20808233 Msa0723210:CDS
TGAAATAAAATTTAAACTAG+AGG 0.550858 5_1:-20806159 None:intergenic
GTTTGAATCAGGTCTTCCAT+TGG 0.554246 5_1:-20807593 None:intergenic
GTATAAATAAAGAAGAACAA+TGG 0.554764 5_1:-20806553 None:intergenic
ATAATGGCACATTTGTTGGT+GGG 0.555933 5_1:+20808662 Msa0723210:three_prime_UTR
GATGTTAGCAAGTTTAACAA+AGG 0.558526 5_1:-20805830 None:intergenic
GATGGTGATTGGGATAAGGC+TGG 0.558637 5_1:+20808201 Msa0723210:CDS
GGACAAAATTGTATCACCAA+TGG 0.563168 5_1:+20807577 Msa0723210:CDS
TTCCAAGTATCTATAGGCCC+AGG 0.563711 5_1:-20808489 None:intergenic
ACATGTGGAACAATCAAAGA+AGG 0.566099 5_1:+20807556 Msa0723210:CDS
TGAATTAAGGATACAAGAGA+TGG 0.569147 5_1:-20805792 None:intergenic
CAAAATGAAAAGTACATCAA+AGG 0.571346 5_1:+20806458 Msa0723210:exon
CTATGAGCAGAAAACAAAGT+AGG 0.577669 5_1:+20806411 Msa0723210:five_prime_UTR
AACCATGATCCCAAGCACTT+AGG 0.579594 5_1:+20805911 Msa0723210:five_prime_UTR
ATGAAGAAGTGGGATTGGAA+GGG 0.584115 5_1:+20808531 Msa0723210:CDS
ATACCTGCATAATGTGGAGG+TGG 0.587072 5_1:-20806633 None:intergenic
TCTCCACCTCCACATTATGC+AGG 0.592353 5_1:+20806630 Msa0723210:CDS
GAGTATAACCCAAGAAACCT+AGG 0.593290 5_1:+20806073 Msa0723210:five_prime_UTR
ACCTTATGGAAAGGGTGAGA+AGG 0.593453 5_1:+20808242 Msa0723210:CDS
TTATATAGTGGAACAACATG+TGG 0.594064 5_1:+20807541 Msa0723210:CDS
ATGTGGAGGTGGAGAAGTAG+TGG 0.594951 5_1:-20806622 None:intergenic
CTAGACAAGGAAAATGTTTG+GGG 0.599938 5_1:+20808710 Msa0723210:three_prime_UTR
ATGATCTTGGTGTGTTGAGG+AGG 0.601958 5_1:-20806009 None:intergenic
TCTTGGTGTGTTGAGGAGGG+AGG 0.606448 5_1:-20806005 None:intergenic
CAATCACCATCAAAATGAGA+AGG 0.607997 5_1:-20808189 None:intergenic
AAGAAATGGCGACCAATACA+CGG 0.614637 5_1:-20807842 None:intergenic
TTTCCATTGAAGTAGTCACA+TGG 0.617272 5_1:-20807493 None:intergenic
TTCGGAACACCGTTAGCGAA+CGG 0.617341 5_1:-20808432 None:intergenic
AACATGATCTTGGTGTGTTG+AGG 0.620460 5_1:-20806012 None:intergenic
ATTTAATTGAGGAAACTCAC+CGG 0.624932 5_1:-20806041 None:intergenic
GTCTTAATAGCATAATTGAG+AGG 0.633582 5_1:+20808118 Msa0723210:CDS
AACATACCTGCATAATGTGG+AGG 0.634009 5_1:-20806636 None:intergenic
TTGGCATTGTTGAGACCAGA+TGG 0.642859 5_1:+20808381 Msa0723210:CDS
AAAGTGTGACACAAGGACCT+AGG 0.643102 5_1:-20806090 None:intergenic
GGGATTGGAAGGGACAGCCA+AGG 0.651431 5_1:+20808541 Msa0723210:CDS
ATTAACATACCTGCATAATG+TGG 0.654106 5_1:-20806639 None:intergenic
CCATTCTCATAATAAACCGA+AGG 0.655142 5_1:-20808003 None:intergenic
TGATCTTGGTGTGTTGAGGA+GGG 0.671076 5_1:-20806008 None:intergenic
ACCAATGTGAACCTTTATGG+TGG 0.671348 5_1:-20807789 None:intergenic
TCCACCATAAAGGTTCACAT+TGG 0.672090 5_1:+20807788 Msa0723210:CDS
AAATTGGCGAAGGATATGGA+TGG 0.672881 5_1:+20807934 Msa0723210:CDS
GAGGTGGAGAAGTAGTGGCA+TGG 0.690355 5_1:-20806617 None:intergenic
ATTGAAGAAAGTGTGACACA+AGG 0.695094 5_1:-20806097 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAAAAAGTTAAGAT+TGG - chr5_1:20805867-20805886 None:intergenic 10.0%
!!! AAATATAAATTTTCATATCA+TGG - chr5_1:20806132-20806151 None:intergenic 10.0%
!! ATGATATGAAAATTTATATT+TGG + chr5_1:20806131-20806150 Msa0723210:five_prime_UTR 10.0%
!!! CAAATTAGTAAATATTTTTT+TGG + chr5_1:20806901-20806920 Msa0723210:intron 10.0%
!! TTTAAATAAATCTAATCAAA+GGG - chr5_1:20807381-20807400 None:intergenic 10.0%
!!! TAATAAACATAATCTTTTTA+TGG - chr5_1:20808619-20808638 None:intergenic 10.0%
!!! AAATCAGTTTTTAATTTTTA+TGG - chr5_1:20808810-20808829 None:intergenic 10.0%
!! TCTAATTTATGATTTAATTG+AGG - chr5_1:20806055-20806074 None:intergenic 15.0%
!! TGAAATAAAATTTAAACTAG+AGG - chr5_1:20806162-20806181 None:intergenic 15.0%
!! AAAGAATAAAGTACAATATA+TGG - chr5_1:20806207-20806226 None:intergenic 15.0%
!! TATAAATAAAGAAGAACAAT+GGG - chr5_1:20806555-20806574 None:intergenic 15.0%
!! TTAAATAAATCTAATCAAAG+GGG - chr5_1:20807380-20807399 None:intergenic 15.0%
!! GTTTAAATAAATCTAATCAA+AGG - chr5_1:20807382-20807401 None:intergenic 15.0%
!! AAATGTATTGTATTAAAACT+TGG - chr5_1:20807898-20807917 None:intergenic 15.0%
!!! AAGTTTTAATACAATACATT+TGG + chr5_1:20807897-20807916 Msa0723210:CDS 15.0%
!! AAAGTTTAATATCTACATAT+TGG - chr5_1:20808753-20808772 None:intergenic 15.0%
!!! ATTTTTGATTAGATGAATTA+TGG + chr5_1:20808832-20808851 Msa0723210:three_prime_UTR 15.0%
!! GTATAAATAAAGAAGAACAA+TGG - chr5_1:20806556-20806575 None:intergenic 20.0%
!! TTTATAGAGGAAAAATGATT+AGG + chr5_1:20806751-20806770 Msa0723210:intron 20.0%
!! TAATTAAATGAGGTAATCAT+TGG + chr5_1:20806875-20806894 Msa0723210:intron 20.0%
!! AATATATCTTTCGATAAATC+TGG - chr5_1:20807094-20807113 None:intergenic 20.0%
!!! TTTTAACTCATTTTGTCTAT+CGG + chr5_1:20807127-20807146 Msa0723210:intron 20.0%
!!! TTAAAAGTGTAAAGAGTTTT+CGG - chr5_1:20807287-20807306 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG + chr5_1:20808083-20808102 Msa0723210:CDS 20.0%
!! TAATAAATTAACAAGCTAAG+AGG - chr5_1:20808585-20808604 None:intergenic 20.0%
! AAAGAGAAATCCATGAATTA+AGG - chr5_1:20805808-20805827 None:intergenic 25.0%
!!! CACAAATTTCTTGATGATTT+TGG - chr5_1:20805967-20805986 None:intergenic 25.0%
!!! TTTACTTCACGCAAATTTTT+TGG + chr5_1:20806337-20806356 Msa0723210:five_prime_UTR 25.0%
!! CAAAATGAAAAGTACATCAA+AGG + chr5_1:20806458-20806477 Msa0723210:exon 25.0%
! AGTAAAAGATGAAGTGTATA+TGG - chr5_1:20806516-20806535 None:intergenic 25.0%
! ATTCACACTTGAAAATGATT+AGG + chr5_1:20806723-20806742 Msa0723210:intron 25.0%
!!! TGATTAGGTGTTTTTTATAG+AGG + chr5_1:20806738-20806757 Msa0723210:intron 25.0%
! TGAACTGAACAATAAAATAG+TGG + chr5_1:20806777-20806796 Msa0723210:intron 25.0%
! GAACTGAACAATAAAATAGT+GGG + chr5_1:20806778-20806797 Msa0723210:intron 25.0%
! TTCAACTACTCTAATTAACA+TGG - chr5_1:20806819-20806838 None:intergenic 25.0%
! TTTACAAATCACCACAATTA+AGG + chr5_1:20806991-20807010 Msa0723210:intron 25.0%
! TATCGAAAGATATATTTCGA+TGG + chr5_1:20807098-20807117 Msa0723210:intron 25.0%
! TTTAATGAGTGTGTCTTATA+AGG + chr5_1:20807302-20807321 Msa0723210:intron 25.0%
!!! TTGTATATCAGAGGTTTTAA+AGG - chr5_1:20807334-20807353 None:intergenic 25.0%
!! AAATGGATTCATGACAATAA+AGG + chr5_1:20807514-20807533 Msa0723210:CDS 25.0%
! AATAAAGGTCCTTTATATAG+TGG + chr5_1:20807529-20807548 Msa0723210:CDS 25.0%
!! AACAAACACATATCTTTTGT+TGG + chr5_1:20807694-20807713 Msa0723210:CDS 25.0%
! TTGGTTTATTAATGTTCCTT+CGG + chr5_1:20807987-20808006 Msa0723210:CDS 25.0%
! AAGAAAAAAGCAAATGCATT+TGG + chr5_1:20808324-20808343 Msa0723210:CDS 25.0%
TGAATTAAGGATACAAGAGA+TGG - chr5_1:20805795-20805814 None:intergenic 30.0%
TCTCTTGTATCCTTAATTCA+TGG + chr5_1:20805795-20805814 Msa0723210:five_prime_UTR 30.0%
GATGTTAGCAAGTTTAACAA+AGG - chr5_1:20805833-20805852 None:intergenic 30.0%
!! ATTTTGGAGAAAAGGAGTTT+TGG - chr5_1:20805951-20805970 None:intergenic 30.0%
!! TCTTGATGATTTTGGAGAAA+AGG - chr5_1:20805959-20805978 None:intergenic 30.0%
ATTTAATTGAGGAAACTCAC+CGG - chr5_1:20806044-20806063 None:intergenic 30.0%
TGCACACTTATCTATATTTG+AGG - chr5_1:20806248-20806267 None:intergenic 30.0%
!! TCTTTTGTGATTGTTCTAAG+TGG - chr5_1:20806387-20806406 None:intergenic 30.0%
ATGAAGTGTATATGGAAGAA+GGG - chr5_1:20806508-20806527 None:intergenic 30.0%
! GAGATTTTGTAAGAGACAAA+GGG - chr5_1:20806584-20806603 None:intergenic 30.0%
! TGAGATTTTGTAAGAGACAA+AGG - chr5_1:20806585-20806604 None:intergenic 30.0%
ATTAACATACCTGCATAATG+TGG - chr5_1:20806642-20806661 None:intergenic 30.0%
TTAAACACTTATAGTGAGAC+AGG + chr5_1:20806958-20806977 Msa0723210:intron 30.0%
TATCGGAATATATGTTTCGA+TGG + chr5_1:20807144-20807163 Msa0723210:intron 30.0%
AACCATCTACAAAATTGTCA+CGG + chr5_1:20807209-20807228 Msa0723210:intron 30.0%
AAACCTCTGATATACAATCA+TGG + chr5_1:20807337-20807356 Msa0723210:intron 30.0%
AACCTCTGATATACAATCAT+GGG + chr5_1:20807338-20807357 Msa0723210:intron 30.0%
CATGTTGTTCCACTATATAA+AGG - chr5_1:20807541-20807560 None:intergenic 30.0%
! TTATATAGTGGAACAACATG+TGG + chr5_1:20807541-20807560 Msa0723210:CDS 30.0%
TAGTAAAGGTAGTTTGAATC+AGG - chr5_1:20807607-20807626 None:intergenic 30.0%
CAAACTACCTTTACTATAGA+TGG + chr5_1:20807611-20807630 Msa0723210:CDS 30.0%
TTGGTTTCCATCTATAGTAA+AGG - chr5_1:20807621-20807640 None:intergenic 30.0%
!!! TGTGTTTGTTTTTGCTTAGT+TGG - chr5_1:20807684-20807703 None:intergenic 30.0%
TACAAAGAAGAGACTCAAAT+TGG - chr5_1:20807738-20807757 None:intergenic 30.0%
AACTGTTTCAAAACCTAAAC+CGG + chr5_1:20807765-20807784 Msa0723210:CDS 30.0%
TATTGTATTAAAACTTGGCC+CGG - chr5_1:20807893-20807912 None:intergenic 30.0%
GGTTTATTATGAGAATGGTT+TGG + chr5_1:20808008-20808027 Msa0723210:CDS 30.0%
!!! TATGAGAATGGTTTGGTTTT+AGG + chr5_1:20808015-20808034 Msa0723210:CDS 30.0%
TTAGGATGCTTAAATTGTTC+CGG + chr5_1:20808033-20808052 Msa0723210:CDS 30.0%
GGTCTTAATTACACTGATAT+TGG + chr5_1:20808060-20808079 Msa0723210:CDS 30.0%
TTCCATTAAGAAAGGCTTTA+AGG + chr5_1:20808091-20808110 Msa0723210:CDS 30.0%
GTCTTAATAGCATAATTGAG+AGG + chr5_1:20808118-20808137 Msa0723210:CDS 30.0%
! TTTTCACCTTCTCATTTTGA+TGG + chr5_1:20808183-20808202 Msa0723210:CDS 30.0%
!!! TTCTCATTTTGATGGTGATT+GGG + chr5_1:20808191-20808210 Msa0723210:CDS 30.0%
TGAGAAGGAAATTGGAAAAA+TGG + chr5_1:20808257-20808276 Msa0723210:CDS 30.0%
AAATGGAAAGTGAGATTAGA+AGG + chr5_1:20808274-20808293 Msa0723210:CDS 30.0%
AAAAAAGCAAATGCATTTGG+AGG + chr5_1:20808327-20808346 Msa0723210:CDS 30.0%
AAAAAGCAAATGCATTTGGA+GGG + chr5_1:20808328-20808347 Msa0723210:CDS 30.0%
! CAATCATTTTGCACATACTT+CGG - chr5_1:20808453-20808472 None:intergenic 30.0%
TGCAAAATGATTGTGTTCAT+TGG + chr5_1:20808460-20808479 Msa0723210:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG - chr5_1:20808498-20808517 None:intergenic 30.0%
!!! TTGGTTTTAGTTTCTAGACA+AGG + chr5_1:20808697-20808716 Msa0723210:three_prime_UTR 30.0%
TTCTAGACAAGGAAAATGTT+TGG + chr5_1:20808708-20808727 Msa0723210:three_prime_UTR 30.0%
TCTAGACAAGGAAAATGTTT+GGG + chr5_1:20808709-20808728 Msa0723210:three_prime_UTR 30.0%
! CCAAGATCATGTTGATTAAC+CGG + chr5_1:20806022-20806041 Msa0723210:five_prime_UTR 35.0%
! ATTGAAGAAAGTGTGACACA+AGG - chr5_1:20806100-20806119 None:intergenic 35.0%
!!! CTTCACGCAAATTTTTTGGA+AGG + chr5_1:20806341-20806360 Msa0723210:five_prime_UTR 35.0%
CTATGAGCAGAAAACAAAGT+AGG + chr5_1:20806411-20806430 Msa0723210:five_prime_UTR 35.0%
AGCAGAAAACAAAGTAGGAA+AGG + chr5_1:20806416-20806435 Msa0723210:five_prime_UTR 35.0%
! GGAAAGGAAGCACAAAAAAA+TGG + chr5_1:20806432-20806451 Msa0723210:five_prime_UTR 35.0%
GATGAAGTGTATATGGAAGA+AGG - chr5_1:20806509-20806528 None:intergenic 35.0%
GAGTAGTTGAAAGTAGTAGT+TGG + chr5_1:20806828-20806847 Msa0723210:intron 35.0%
! ATCACCACAATTAAGGCATT+TGG + chr5_1:20806998-20807017 Msa0723210:intron 35.0%
TACCTATACCTGATATCATG+AGG - chr5_1:20807034-20807053 None:intergenic 35.0%
!! TTCCTGTTTTAAGCAATACG+TGG + chr5_1:20807174-20807193 Msa0723210:intron 35.0%
TTCCACGTATTGCTTAAAAC+AGG - chr5_1:20807179-20807198 None:intergenic 35.0%
TTTGTAGATGGTTGCTCTTT+AGG - chr5_1:20807202-20807221 None:intergenic 35.0%
! AACCGTGACAATTTTGTAGA+TGG - chr5_1:20807214-20807233 None:intergenic 35.0%
! TTGTCACGGTTTAGATGTTT+TGG + chr5_1:20807223-20807242 Msa0723210:intron 35.0%
! TGTCACGGTTTAGATGTTTT+GGG + chr5_1:20807224-20807243 Msa0723210:intron 35.0%
TTTCCATTGAAGTAGTCACA+TGG - chr5_1:20807496-20807515 None:intergenic 35.0%
GTGACTACTTCAATGGAAAA+TGG + chr5_1:20807497-20807516 Msa0723210:CDS 35.0%
ACATGTGGAACAATCAAAGA+AGG + chr5_1:20807556-20807575 Msa0723210:CDS 35.0%
GGACAAAATTGTATCACCAA+TGG + chr5_1:20807577-20807596 Msa0723210:CDS 35.0%
ATGCAACATTATCCGTGTAT+TGG + chr5_1:20807830-20807849 Msa0723210:CDS 35.0%
GGTGATGAAAGATCAAAATC+CGG + chr5_1:20807871-20807890 Msa0723210:CDS 35.0%
GTGATGAAAGATCAAAATCC+GGG + chr5_1:20807872-20807891 Msa0723210:CDS 35.0%
GACTTGATTGTGTTATCGTT+TGG + chr5_1:20807961-20807980 Msa0723210:CDS 35.0%
TTGTGTTATCGTTTGGCAAT+TGG + chr5_1:20807968-20807987 Msa0723210:CDS 35.0%
CCATTCTCATAATAAACCGA+AGG - chr5_1:20808006-20808025 None:intergenic 35.0%
CCTTCGGTTTATTATGAGAA+TGG + chr5_1:20808003-20808022 Msa0723210:CDS 35.0%
TGCTTAAATTGTTCCGGTTT+CGG + chr5_1:20808039-20808058 Msa0723210:CDS 35.0%
AGTGTAATTAAGACCGAAAC+CGG - chr5_1:20808055-20808074 None:intergenic 35.0%
GTCCTTAAAGCCTTTCTTAA+TGG - chr5_1:20808096-20808115 None:intergenic 35.0%
ATTGAGAGGAAAGTTGCTAA+AGG + chr5_1:20808132-20808151 Msa0723210:CDS 35.0%
TTGAGAGGAAAGTTGCTAAA+GGG + chr5_1:20808133-20808152 Msa0723210:CDS 35.0%
CAATCACCATCAAAATGAGA+AGG - chr5_1:20808192-20808211 None:intergenic 35.0%
!!! CTTCTCATTTTGATGGTGAT+TGG + chr5_1:20808190-20808209 Msa0723210:CDS 35.0%
!!! TTTTGATGGTGATTGGGATA+AGG + chr5_1:20808197-20808216 Msa0723210:CDS 35.0%
TGTTCAAAGACTGAACCTTA+TGG + chr5_1:20808228-20808247 Msa0723210:CDS 35.0%
AAAGACTGAACCTTATGGAA+AGG + chr5_1:20808233-20808252 Msa0723210:CDS 35.0%
AAGACTGAACCTTATGGAAA+GGG + chr5_1:20808234-20808253 Msa0723210:CDS 35.0%
AAGGATTGAGATTGAAGAAG+TGG + chr5_1:20808293-20808312 Msa0723210:CDS 35.0%
ATTTGGAGGGATTAGATTTG+AGG + chr5_1:20808341-20808360 Msa0723210:CDS 35.0%
! AGGGATTAGATTTGAGGTAA+TGG + chr5_1:20808347-20808366 Msa0723210:CDS 35.0%
!! GGTAATGGATGTAACTGAAT+TGG + chr5_1:20808362-20808381 Msa0723210:CDS 35.0%
AAAGGATTCATATAAGCTCC+TGG - chr5_1:20808411-20808430 None:intergenic 35.0%
! TTCTAGAGATGATGAAGAAG+TGG + chr5_1:20808520-20808539 Msa0723210:CDS 35.0%
TCTAGAGATGATGAAGAAGT+GGG + chr5_1:20808521-20808540 Msa0723210:CDS 35.0%
TCTCTGATCTTCTTCCATAA+TGG + chr5_1:20808646-20808665 Msa0723210:three_prime_UTR 35.0%
TTCCATAATGGCACATTTGT+TGG + chr5_1:20808658-20808677 Msa0723210:three_prime_UTR 35.0%
! ATAATGGCACATTTGTTGGT+GGG + chr5_1:20808662-20808681 Msa0723210:three_prime_UTR 35.0%
CTAGACAAGGAAAATGTTTG+GGG + chr5_1:20808710-20808729 Msa0723210:three_prime_UTR 35.0%
GTTCATGATCCTAAGTGCTT+GGG - chr5_1:20805923-20805942 None:intergenic 40.0%
TGTTCATGATCCTAAGTGCT+TGG - chr5_1:20805924-20805943 None:intergenic 40.0%
! AACATGATCTTGGTGTGTTG+AGG - chr5_1:20806015-20806034 None:intergenic 40.0%
CCGGTTAATCAACATGATCT+TGG - chr5_1:20806025-20806044 None:intergenic 40.0%
GAGTATAACCCAAGAAACCT+AGG + chr5_1:20806073-20806092 Msa0723210:five_prime_UTR 40.0%
TGGAAGAAGGGTCTTAGAAA+GGG - chr5_1:20806496-20806515 None:intergenic 40.0%
ATGGAAGAAGGGTCTTAGAA+AGG - chr5_1:20806497-20806516 None:intergenic 40.0%
AACATACCTGCATAATGTGG+AGG - chr5_1:20806639-20806658 None:intergenic 40.0%
GAGACCAAATGCCTTAATTG+TGG - chr5_1:20807005-20807024 None:intergenic 40.0%
TCATAACGCCTCATGATATC+AGG + chr5_1:20807023-20807042 Msa0723210:intron 40.0%
GTGCTTCATAGCAATATGGA+GGG - chr5_1:20807070-20807089 None:intergenic 40.0%
AGTGCTTCATAGCAATATGG+AGG - chr5_1:20807071-20807090 None:intergenic 40.0%
! TGGAGTGCTTCATAGCAATA+TGG - chr5_1:20807074-20807093 None:intergenic 40.0%
CACCCATGATTGTATATCAG+AGG - chr5_1:20807343-20807362 None:intergenic 40.0%
CATCCATGTGACTACTTCAA+TGG + chr5_1:20807490-20807509 Msa0723210:CDS 40.0%
GTTTGAATCAGGTCTTCCAT+TGG - chr5_1:20807596-20807615 None:intergenic 40.0%
CTTGGAAGATTGCACTCATT+TGG - chr5_1:20807640-20807659 None:intergenic 40.0%
CAAATGAGTGCAATCTTCCA+AGG + chr5_1:20807638-20807657 Msa0723210:CDS 40.0%
! AAAGTGTCAGGTTCAAACCT+TGG - chr5_1:20807658-20807677 None:intergenic 40.0%
!! CTTAGTTGGAGAAAAGTGTC+AGG - chr5_1:20807670-20807689 None:intergenic 40.0%
TCCACCATAAAGGTTCACAT+TGG + chr5_1:20807788-20807807 Msa0723210:CDS 40.0%
ACCAATGTGAACCTTTATGG+TGG - chr5_1:20807792-20807811 None:intergenic 40.0%
ACCATAAAGGTTCACATTGG+TGG + chr5_1:20807791-20807810 Msa0723210:CDS 40.0%
AAGAAATGGCGACCAATACA+CGG - chr5_1:20807845-20807864 None:intergenic 40.0%
TCATCACCTTGCACAAGAAA+TGG - chr5_1:20807859-20807878 None:intergenic 40.0%
GGATCGCGTTAACGAAAAAT+TGG + chr5_1:20807918-20807937 Msa0723210:CDS 40.0%
CGTTAACGAAAAATTGGCGA+AGG + chr5_1:20807924-20807943 Msa0723210:CDS 40.0%
CGAAAAATTGGCGAAGGATA+TGG + chr5_1:20807930-20807949 Msa0723210:CDS 40.0%
AAATTGGCGAAGGATATGGA+TGG + chr5_1:20807934-20807953 Msa0723210:CDS 40.0%
AGGAAAGTTGCTAAAGGGAA+TGG + chr5_1:20808138-20808157 Msa0723210:CDS 40.0%
GTTGCTAAAGGGAATGGAAT+TGG + chr5_1:20808144-20808163 Msa0723210:CDS 40.0%
! AATCCTTTTCCGTTCGCTAA+CGG + chr5_1:20808423-20808442 Msa0723210:CDS 40.0%
AGATGATGAAGAAGTGGGAT+TGG + chr5_1:20808526-20808545 Msa0723210:CDS 40.0%
ATGAAGAAGTGGGATTGGAA+GGG + chr5_1:20808531-20808550 Msa0723210:CDS 40.0%
! CACCAACAAATGTGCCATTA+TGG - chr5_1:20808663-20808682 None:intergenic 40.0%
! CATAATGGCACATTTGTTGG+TGG + chr5_1:20808661-20808680 Msa0723210:three_prime_UTR 40.0%
!!! TGGTGGGAGACTTTTGTTTT+TGG + chr5_1:20808678-20808697 Msa0723210:three_prime_UTR 40.0%
! TTTGGGGTTTGAAAGCAACT+TGG + chr5_1:20808726-20808745 Msa0723210:three_prime_UTR 40.0%
AACCATGATCCCAAGCACTT+AGG + chr5_1:20805911-20805930 Msa0723210:five_prime_UTR 45.0%
ATCCTAAGTGCTTGGGATCA+TGG - chr5_1:20805916-20805935 None:intergenic 45.0%
! TGATCTTGGTGTGTTGAGGA+GGG - chr5_1:20806011-20806030 None:intergenic 45.0%
! ATGATCTTGGTGTGTTGAGG+AGG - chr5_1:20806012-20806031 None:intergenic 45.0%
CACAAGGACCTAGGTTTCTT+GGG - chr5_1:20806084-20806103 None:intergenic 45.0%
ACACAAGGACCTAGGTTTCT+TGG - chr5_1:20806085-20806104 None:intergenic 45.0%
AAAGTGTGACACAAGGACCT+AGG - chr5_1:20806093-20806112 None:intergenic 45.0%
ATACCTGCATAATGTGGAGG+TGG - chr5_1:20806636-20806655 None:intergenic 45.0%
CGCCTCATGATATCAGGTAT+AGG + chr5_1:20807029-20807048 Msa0723210:intron 45.0%
!! ACGGTTTAGATGTTTTGGGC+CGG + chr5_1:20807228-20807247 Msa0723210:intron 45.0%
!! AGATGTTTTGGGCCGGTTAA+TGG + chr5_1:20807235-20807254 Msa0723210:intron 45.0%
TCGGTGTAACTAAACTCCGA+GGG - chr5_1:20807268-20807287 None:intergenic 45.0%
! TTCGGTGTAACTAAACTCCG+AGG - chr5_1:20807269-20807288 None:intergenic 45.0%
!! TGTGAACCTTTATGGTGGAC+CGG - chr5_1:20807787-20807806 None:intergenic 45.0%
GCCACCAATGTGAACCTTTA+TGG - chr5_1:20807795-20807814 None:intergenic 45.0%
ACCTTATGGAAAGGGTGAGA+AGG + chr5_1:20808242-20808261 Msa0723210:CDS 45.0%
TCCTTCTCACCCTTTCCATA+AGG - chr5_1:20808246-20808265 None:intergenic 45.0%
GGAAAGGGTGAGAAGGAAAT+TGG + chr5_1:20808249-20808268 Msa0723210:CDS 45.0%
TTGGCATTGTTGAGACCAGA+TGG + chr5_1:20808381-20808400 Msa0723210:CDS 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG + chr5_1:20808471-20808490 Msa0723210:CDS 45.0%
! GTGTTCATTGGTGTTTGCCT+GGG + chr5_1:20808472-20808491 Msa0723210:CDS 45.0%
TTCCAAGTATCTATAGGCCC+AGG - chr5_1:20808492-20808511 None:intergenic 45.0%
GATGAAGAAGTGGGATTGGA+AGG + chr5_1:20808530-20808549 Msa0723210:CDS 45.0%
ATGTCTCACTCTTCACTCCT+TGG - chr5_1:20808561-20808580 None:intergenic 45.0%
!! TATATATATTTAATTAAATG+AGG + chr5_1:20806865-20806884 Msa0723210:intron 5.0%
!!! TTACTATTTTAATATATTAT+TGG - chr5_1:20808873-20808892 None:intergenic 5.0%
ATGTGGAGGTGGAGAAGTAG+TGG - chr5_1:20806625-20806644 None:intergenic 50.0%
TCTCCACCTCCACATTATGC+AGG + chr5_1:20806630-20806649 Msa0723210:CDS 50.0%
!! CCTTTATGGTGGACCGGTTT+AGG - chr5_1:20807781-20807800 None:intergenic 50.0%
CCTAAACCGGTCCACCATAA+AGG + chr5_1:20807778-20807797 Msa0723210:CDS 50.0%
TGGTCGCCATTTCTTGTGCA+AGG + chr5_1:20807850-20807869 Msa0723210:CDS 50.0%
GATGGTGATTGGGATAAGGC+TGG + chr5_1:20808201-20808220 Msa0723210:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG + chr5_1:20808390-20808409 Msa0723210:CDS 50.0%
TAAGCTCCTGGATGACCATC+TGG - chr5_1:20808399-20808418 None:intergenic 50.0%
ACACCGTTAGCGAACGGAAA+AGG - chr5_1:20808429-20808448 None:intergenic 50.0%
TTCGGAACACCGTTAGCGAA+CGG - chr5_1:20808435-20808454 None:intergenic 50.0%
TGCCTGGGCCTATAGATACT+TGG + chr5_1:20808487-20808506 Msa0723210:CDS 50.0%
GTGTTGAGGAGGGAGGATTC+AGG - chr5_1:20806001-20806020 None:intergenic 55.0%
! TCTTGGTGTGTTGAGGAGGG+AGG - chr5_1:20806008-20806027 None:intergenic 55.0%
GAGGTGGAGAAGTAGTGGCA+TGG - chr5_1:20806620-20806639 None:intergenic 55.0%
AACTAAACTCCGAGGGCCGA+GGG - chr5_1:20807261-20807280 None:intergenic 55.0%
TAACTAAACTCCGAGGGCCG+AGG - chr5_1:20807262-20807281 None:intergenic 55.0%
TTGGGCCGGTTAATGGCCCT+CGG + chr5_1:20807242-20807261 Msa0723210:intron 60.0%
GGGATTGGAAGGGACAGCCA+AGG + chr5_1:20808541-20808560 Msa0723210:CDS 60.0%
GAGGGCCGAGGGCCATTAAC+CGG - chr5_1:20807250-20807269 None:intergenic 65.0%
GGTTAATGGCCCTCGGCCCT+CGG + chr5_1:20807249-20807268 Msa0723210:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20805779 20808904 20805779 ID=Msa0723210;Name=Msa0723210
chr5_1 mRNA 20805779 20808904 20805779 ID=Msa0723210-mRNA-1;Parent=Msa0723210;Name=Msa0723210-mRNA-1;_AED=0.19;_eAED=0.19;_QI=683|1|1|1|1|1|2|329|433
chr5_1 exon 20805779 20806651 20805779 ID=Msa0723210-mRNA-1:exon:7949;Parent=Msa0723210-mRNA-1
chr5_1 exon 20807464 20808904 20807464 ID=Msa0723210-mRNA-1:exon:7950;Parent=Msa0723210-mRNA-1
chr5_1 five_prime_UTR 20805779 20806461 20805779 ID=Msa0723210-mRNA-1:five_prime_utr;Parent=Msa0723210-mRNA-1
chr5_1 CDS 20806462 20806651 20806462 ID=Msa0723210-mRNA-1:cds;Parent=Msa0723210-mRNA-1
chr5_1 CDS 20807464 20808575 20807464 ID=Msa0723210-mRNA-1:cds;Parent=Msa0723210-mRNA-1
chr5_1 three_prime_UTR 20808576 20808904 20808576 ID=Msa0723210-mRNA-1:three_prime_utr;Parent=Msa0723210-mRNA-1
Gene Sequence

>Msa0723210

ATGAAAAGTACATCAAAGGATGATTCTTTTTCCCTTTCTAAGACCCTTCTTCCATATACACTTCATCTTTTACTTCTCATAGCTCTCTTACCCATTGTTCTTCTTTATTTATACTTTTACCCTTTGTCTCTTACAAAATCTCAAACTCAGCTTCACCATGCCACTACTTCTCCACCTCCACATTATGCAGTAAATGACAAACACAAAATTGATGAGCATCCATGTGACTACTTCAATGGAAAATGGATTCATGACAATAAAGGTCCTTTATATAGTGGAACAACATGTGGAACAATCAAAGAAGGACAAAATTGTATCACCAATGGAAGACCTGATTCAAACTACCTTTACTATAGATGGAAACCAAATGAGTGCAATCTTCCAAGGTTTGAACCTGACACTTTTCTCCAACTAAGCAAAAACAAACACATATCTTTTGTTGGTGATTCTTTAGCTAGAAACCAATTTGAGTCTCTTCTTTGTATGTTAGCAACTGTTTCAAAACCTAAACCGGTCCACCATAAAGGTTCACATTGGTGGCATTTTTCTTCTCATAATGCAACATTATCCGTGTATTGGTCGCCATTTCTTGTGCAAGGTGATGAAAGATCAAAATCCGGGCCAAGTTTTAATACAATACATTTGGATCGCGTTAACGAAAAATTGGCGAAGGATATGGATGGAATCGACTTGATTGTGTTATCGTTTGGCAATTGGTTTATTAATGTTCCTTCGGTTTATTATGAGAATGGTTTGGTTTTAGGATGCTTAAATTGTTCCGGTTTCGGTCTTAATTACACTGATATTGGTTTTTATGTTCCATTAAGAAAGGCTTTAAGGACTTGTCTTAATAGCATAATTGAGAGGAAAGTTGCTAAAGGGAATGGAATTGGTGTAATTGTGAAAACTTTTTCACCTTCTCATTTTGATGGTGATTGGGATAAGGCTGGTACATGTTCAAAGACTGAACCTTATGGAAAGGGTGAGAAGGAAATTGGAAAAATGGAAAGTGAGATTAGAAGGATTGAGATTGAAGAAGTGGAAAATGCTAAGAAAAAAGCAAATGCATTTGGAGGGATTAGATTTGAGGTAATGGATGTAACTGAATTGGCATTGTTGAGACCAGATGGTCATCCAGGAGCTTATATGAATCCTTTTCCGTTCGCTAACGGTGTTCCGAAGTATGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGGCCTATAGATACTTGGAATGAGATTTTTCTAGAGATGATGAAGAAGTGGGATTGGAAGGGACAGCCAAGGAGTGAAGAGTGA
Protein sequence

>Msa0723210

MKSTSKDDSFSLSKTLLPYTLHLLLLIALLPIVLLYLYFYPLSLTKSQTQLHHATTSPPPHYAVNDKHKIDEHPCDYFNGKWIHDNKGPLYSGTTCGTIKEGQNCITNGRPDSNYLYYRWKPNECNLPRFEPDTFLQLSKNKHISFVGDSLARNQFESLLCMLATVSKPKPVHHKGSHWWHFSSHNATLSVYWSPFLVQGDERSKSGPSFNTIHLDRVNEKLAKDMDGIDLIVLSFGNWFINVPSVYYENGLVLGCLNCSGFGLNYTDIGFYVPLRKALRTCLNSIIERKVAKGNGIGVIVKTFSPSHFDGDWDKAGTCSKTEPYGKGEKEIGKMESEIRRIEIEEVENAKKKANAFGGIRFEVMDVTELALLRPDGHPGAYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFLEMMKKWDWKGQPRSEE*