Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723050 | XP_003613278.1 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723050 | sp|K4PW38|RSS3_ORYSJ | 36.444 | 225 | 105 | 7 | 14 | 227 | 31 | 228 | 8.58e-36 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723050 | G7K2R0 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000190 | Msa0723050 | 0.822515 | 2.273747e-53 | -8.615850e-47 |
| Msa0017510 | Msa0723050 | 0.827429 | 1.572129e-54 | -8.615850e-47 |
| Msa0029890 | Msa0723050 | 0.821821 | 3.293586e-53 | -8.615850e-47 |
| Msa0101810 | Msa0723050 | -0.819393 | 1.189125e-52 | -8.615850e-47 |
| Msa0110970 | Msa0723050 | 0.806682 | 7.305025e-50 | -8.615850e-47 |
| Msa0641360 | Msa0723050 | 0.801134 | 1.039103e-48 | -8.615850e-47 |
| Msa0657970 | Msa0723050 | 0.800271 | 1.558587e-48 | -8.615850e-47 |
| Msa0688770 | Msa0723050 | 0.805722 | 1.163999e-49 | -8.615850e-47 |
| Msa0719490 | Msa0723050 | -0.822692 | 2.067478e-53 | -8.615850e-47 |
| Msa0722780 | Msa0723050 | 0.966226 | 1.224203e-125 | -8.615850e-47 |
| Msa0722850 | Msa0723050 | 0.943356 | 1.394212e-102 | -8.615850e-47 |
| Msa0723000 | Msa0723050 | 0.985647 | 3.192917e-164 | -8.615850e-47 |
| Msa0723050 | Msa0756630 | -0.819000 | 1.460852e-52 | -8.615850e-47 |
| Msa0723050 | Msa0802470 | -0.825353 | 4.909642e-54 | -8.615850e-47 |
| Msa0723050 | Msa0802860 | 0.803515 | 3.359475e-49 | -8.615850e-47 |
| Msa0723050 | Msa0828560 | 0.801941 | 7.100514e-49 | -8.615850e-47 |
| Msa0723050 | Msa0843220 | 0.915374 | 6.353139e-85 | -8.615850e-47 |
| Msa0723050 | Msa0843780 | -0.802896 | 4.512336e-49 | -8.615850e-47 |
| Msa0723050 | Msa0880140 | 0.829425 | 5.185469e-55 | -8.615850e-47 |
| Msa0723050 | Msa0896970 | 0.808541 | 2.943514e-50 | -8.615850e-47 |
| Msa0723050 | Msa0967380 | 0.800546 | 1.369978e-48 | -8.615850e-47 |
| Msa0723050 | Msa0977180 | -0.810278 | 1.247776e-50 | -8.615850e-47 |
| Msa0723050 | Msa1009000 | 0.810737 | 9.935436e-51 | -8.615850e-47 |
| Msa0723050 | Msa1075220 | -0.815983 | 6.990630e-52 | -8.615850e-47 |
| Msa0723050 | Msa1077150 | 0.809894 | 1.509951e-50 | -8.615850e-47 |
| Msa0723050 | Msa1077180 | 0.838756 | 2.380640e-57 | -8.615850e-47 |
| Msa0723050 | Msa1083410 | 0.805944 | 1.045152e-49 | -8.615850e-47 |
| Msa0723050 | Msa1085300 | 0.805301 | 1.425929e-49 | -8.615850e-47 |
| Msa0723050 | Msa1098560 | 0.841960 | 3.461345e-58 | -8.615850e-47 |
| Msa0723050 | Msa1222970 | 0.825969 | 3.507715e-54 | -8.615850e-47 |
| Msa0723050 | Msa1228210 | 0.813756 | 2.179253e-51 | -8.615850e-47 |
| Msa0723050 | Msa1242220 | 0.818679 | 1.727964e-52 | -8.615850e-47 |
| Msa0723050 | Msa1242230 | 0.821167 | 4.662688e-53 | -8.615850e-47 |
| Msa0723050 | Msa1242240 | 0.805433 | 1.338107e-49 | -8.615850e-47 |
| Msa0723050 | Msa1271280 | 0.814783 | 1.292145e-51 | -8.615850e-47 |
| Msa0723050 | Msa1278840 | 0.818431 | 1.967305e-52 | -8.615850e-47 |
| Msa0723050 | Msa1283840 | 0.807425 | 5.087035e-50 | -8.615850e-47 |
| Msa0723050 | Msa1283860 | 0.808741 | 2.668719e-50 | -8.615850e-47 |
| Msa0723050 | Msa1284260 | 0.826936 | 2.063528e-54 | -8.615850e-47 |
| Msa0723050 | Msa1293770 | 0.805710 | 1.170587e-49 | -8.615850e-47 |
| Msa0723050 | Msa1322860 | 0.832303 | 1.020664e-55 | -8.615850e-47 |
| Msa0723050 | Msa1322890 | 0.824392 | 8.273107e-54 | -8.615850e-47 |
| Msa0723050 | Msa1327050 | 0.823005 | 1.748319e-53 | -8.615850e-47 |
| Msa0723050 | Msa1367080 | 0.817902 | 2.591833e-52 | -8.615850e-47 |
| Msa0723050 | Msa1374550 | 0.863760 | 1.977039e-64 | -8.615850e-47 |
| Msa0125090 | Msa0723050 | 0.801099 | 1.056409e-48 | -8.615850e-47 |
| Msa0125100 | Msa0723050 | 0.810288 | 1.242064e-50 | -8.615850e-47 |
| Msa0140780 | Msa0723050 | 0.815140 | 1.076813e-51 | -8.615850e-47 |
| Msa0157290 | Msa0723050 | 0.827479 | 1.529788e-54 | -8.615850e-47 |
| Msa0169860 | Msa0723050 | 0.816647 | 4.964901e-52 | -8.615850e-47 |
| Msa0202950 | Msa0723050 | 0.819521 | 1.111981e-52 | -8.615850e-47 |
| Msa0203010 | Msa0723050 | 0.802707 | 4.936699e-49 | -8.615850e-47 |
| Msa0220740 | Msa0723050 | 0.803994 | 2.672869e-49 | -8.615850e-47 |
| Msa0221290 | Msa0723050 | 0.803640 | 3.165644e-49 | -8.615850e-47 |
| Msa0385390 | Msa0723050 | 0.810198 | 1.298405e-50 | -8.615850e-47 |
| Msa0392420 | Msa0723050 | 0.838275 | 3.168353e-57 | -8.615850e-47 |
| Msa0392460 | Msa0723050 | 0.835944 | 1.249309e-56 | -8.615850e-47 |
| Msa0392490 | Msa0723050 | 0.840479 | 8.480693e-58 | -8.615850e-47 |
| Msa0465670 | Msa0723050 | -0.811886 | 5.593235e-51 | -8.615850e-47 |
| Msa0465950 | Msa0723050 | 0.830021 | 3.712147e-55 | -8.615850e-47 |
| Msa0480980 | Msa0723050 | 0.815674 | 8.191110e-52 | -8.615850e-47 |
| Msa0243100 | Msa0723050 | 0.811024 | 8.607052e-51 | -8.615850e-47 |
| Msa0284420 | Msa0723050 | 0.816072 | 6.676236e-52 | -8.615850e-47 |
| Msa0287650 | Msa0723050 | 0.805481 | 1.307403e-49 | -8.615850e-47 |
| Msa0344570 | Msa0723050 | 0.825439 | 4.684012e-54 | -8.615850e-47 |
| Msa0487570 | Msa0723050 | 0.841565 | 4.399697e-58 | -8.615850e-47 |
| Msa0487630 | Msa0723050 | 0.845419 | 4.109048e-59 | -8.615850e-47 |
| Msa0523460 | Msa0723050 | 0.804749 | 1.860131e-49 | -8.615850e-47 |
| Msa0535040 | Msa0723050 | 0.814942 | 1.191178e-51 | -8.615850e-47 |
| Msa0548280 | Msa0723050 | -0.821355 | 4.218899e-53 | -8.615850e-47 |
| Msa0581760 | Msa0723050 | 0.817599 | 3.033109e-52 | -8.615850e-47 |
| Msa0591790 | Msa0723050 | 0.807861 | 4.110778e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723050 | MtrunA17_Chr5g0413871 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Msa0723050 | MtrunA17_Chr1g0152981 | 51.185 | 211 | 80 | 5 | 13 | 217 | 11 | 204 | 6.14e-70 | 221 |
| Msa0723050 | MtrunA17_Chr2g0317011 | 38.537 | 205 | 99 | 6 | 14 | 218 | 20 | 197 | 5.12e-36 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0723050 | AT1G60060.1 | 68.681 | 364 | 65 | 10 | 1 | 336 | 1 | 343 | 4.00e-160 | 454 |
| Msa0723050 | AT1G60060.2 | 64.263 | 319 | 65 | 10 | 46 | 336 | 6 | 303 | 1.12e-123 | 360 |
| Msa0723050 | AT5G53900.2 | 38.333 | 240 | 118 | 7 | 14 | 244 | 21 | 239 | 8.39e-39 | 142 |
| Msa0723050 | AT3G15240.2 | 37.073 | 205 | 107 | 4 | 14 | 218 | 34 | 216 | 9.58e-38 | 139 |
| Msa0723050 | AT5G53900.3 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.58e-20 | 89.7 |
| Msa0723050 | AT5G53900.1 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.58e-20 | 89.7 |
| Msa0723050 | AT2G31280.1 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 1.83e-14 | 75.1 |
| Msa0723050 | AT2G31280.3 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 2.08e-14 | 75.1 |
| Msa0723050 | AT2G27230.2 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 2.79e-13 | 71.6 |
| Msa0723050 | AT2G27230.1 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 2.79e-13 | 71.6 |
Find 102 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGCTCCTGCTGCTGTTTA+AGG | 0.126120 | 5_1:-20665987 | Msa0723050:three_prime_UTR |
| CCTAGCTCATCATCAGTTTA+TGG | 0.128324 | 5_1:-20667869 | Msa0723050:CDS |
| ATAAAATAATTAATAATTAT+TGG | 0.163550 | 5_1:+20665943 | None:intergenic |
| CAATCACCAGTGTTTATTTC+AGG | 0.239507 | 5_1:+20667893 | None:intergenic |
| TGCATATTTCAGTTTCTTCT+TGG | 0.255252 | 5_1:-20666160 | Msa0723050:three_prime_UTR |
| TCACATGAGATCTATAACTA+TGG | 0.263874 | 5_1:-20667788 | Msa0723050:CDS |
| CACACATTTGCATGGAATAA+TGG | 0.268865 | 5_1:+20666102 | None:intergenic |
| TTGGGTGGAGGGTAGTTTCT+AGG | 0.275012 | 5_1:+20668356 | None:intergenic |
| AACACAAACACAACATGTTT+TGG | 0.280192 | 5_1:-20666507 | Msa0723050:CDS |
| AGAGAAGGTGTTGTTCAATT+AGG | 0.283607 | 5_1:-20667217 | Msa0723050:CDS |
| AAATTAATTTCTTGATCATT+AGG | 0.297602 | 5_1:+20667589 | None:intergenic |
| CAACGAGCATTGGAATTTAC+AGG | 0.318102 | 5_1:-20666473 | Msa0723050:CDS |
| TATATGCATGCACAGGATAT+TGG | 0.324411 | 5_1:-20667957 | Msa0723050:intron |
| CATTTCCAAGATGATCTTGA+TGG | 0.328838 | 5_1:+20666330 | None:intergenic |
| AGGATATTGGTTTGGGAAGA+TGG | 0.329021 | 5_1:-20667944 | Msa0723050:CDS |
| TCTTGAAACCCTTTAGCTAT+AGG | 0.329720 | 5_1:+20668586 | None:intergenic |
| ATTGATGGAGTTACTTTCAT+TGG | 0.336300 | 5_1:+20666596 | None:intergenic |
| AATGGATCAAACAATAATAA+TGG | 0.339598 | 5_1:-20666311 | Msa0723050:CDS |
| CATCAAGATCATCTTGGAAA+TGG | 0.346285 | 5_1:-20666329 | Msa0723050:CDS |
| TCAGTTCTTGTCTGGTATAA+AGG | 0.362612 | 5_1:-20667381 | Msa0723050:intron |
| GCATGCACAGGATATTGGTT+TGG | 0.384339 | 5_1:-20667952 | Msa0723050:intron |
| GTGAGAGAGTTATTAATGTT+AGG | 0.386113 | 5_1:-20666220 | Msa0723050:three_prime_UTR |
| TCACCAACATCAAGTTGTTC+AGG | 0.392523 | 5_1:+20666392 | None:intergenic |
| GACATCTTGAAGAAAAGTTC+AGG | 0.396788 | 5_1:+20667809 | None:intergenic |
| CATGCACAGGATATTGGTTT+GGG | 0.400265 | 5_1:-20667951 | Msa0723050:intron |
| TCATCATCAGCATACCCTTA+TGG | 0.403770 | 5_1:-20666713 | Msa0723050:CDS |
| TAAAGATACCTATAGCTAAA+GGG | 0.404162 | 5_1:-20668594 | Msa0723050:five_prime_UTR |
| GTTGGTGTGTGCTTATATAA+AGG | 0.405928 | 5_1:-20668648 | Msa0723050:five_prime_UTR |
| GCTTCAAGACTACTCATTGA+TGG | 0.406362 | 5_1:+20666581 | None:intergenic |
| TTAAAGATACCTATAGCTAA+AGG | 0.409220 | 5_1:-20668595 | Msa0723050:five_prime_UTR |
| TGGGAAGCTCAGTTCTTGTC+TGG | 0.414012 | 5_1:-20667389 | Msa0723050:CDS |
| CTAGCTCATCATCAGTTTAT+GGG | 0.418675 | 5_1:-20667868 | Msa0723050:CDS |
| CTCTCTCACTACAATATGTT+AGG | 0.427654 | 5_1:+20666234 | None:intergenic |
| TAGGTATCTTTAATAATAGT+AGG | 0.427738 | 5_1:+20668605 | None:intergenic |
| CCAATCTACTACATTGTACT+TGG | 0.431351 | 5_1:-20666024 | Msa0723050:three_prime_UTR |
| TAAATGTGATATTTCCTTGT+TGG | 0.431856 | 5_1:-20668666 | Msa0723050:five_prime_UTR |
| CATTTCCATCAAGATCATCT+TGG | 0.434289 | 5_1:-20666335 | Msa0723050:CDS |
| TTTACAGCACCCTAGGACTT+GGG | 0.440440 | 5_1:-20667408 | Msa0723050:intron |
| CAAGAGGAAACAGAAGGAAT+TGG | 0.444449 | 5_1:-20668099 | Msa0723050:intron |
| TGTTCTGATGGACCACCATA+AGG | 0.450763 | 5_1:+20666698 | None:intergenic |
| GGTAGTGTATTGTAGTATGA+AGG | 0.452877 | 5_1:-20666139 | Msa0723050:three_prime_UTR |
| CTATGATCTTCTTACTTGGG+TGG | 0.478789 | 5_1:+20668341 | None:intergenic |
| TGATCTTCTTACTTGGGTGG+AGG | 0.479359 | 5_1:+20668344 | None:intergenic |
| GAACAATGGCACAACTTTCA+AGG | 0.484632 | 5_1:-20666680 | Msa0723050:CDS |
| GTTGGATAATGAAGAAGAAG+AGG | 0.487343 | 5_1:-20666423 | Msa0723050:CDS |
| CCAAGTACAATGTAGTAGAT+TGG | 0.489579 | 5_1:+20666024 | None:intergenic |
| AACTGAGCTTCCCAAGTCCT+AGG | 0.490005 | 5_1:+20667398 | None:intergenic |
| AGCTACATTGAAAGCATACC+AGG | 0.501005 | 5_1:-20666755 | Msa0723050:CDS |
| GTTCTGATGGACCACCATAA+GGG | 0.509808 | 5_1:+20666699 | None:intergenic |
| GAGATCTATAACTATGGAGA+AGG | 0.511952 | 5_1:-20667782 | Msa0723050:CDS |
| TAATATAGGTGGGAAGGACA+AGG | 0.515798 | 5_1:-20668136 | Msa0723050:intron |
| TAAGGGTATGCTGATGATGA+TGG | 0.520173 | 5_1:+20666716 | None:intergenic |
| GATCTTCTTACTTGGGTGGA+GGG | 0.521140 | 5_1:+20668345 | None:intergenic |
| TGTGTATAATATAGGTGGGA+AGG | 0.521340 | 5_1:-20668142 | Msa0723050:intron |
| TTGCATCCTTAAACAGCAGC+AGG | 0.525427 | 5_1:+20665981 | None:intergenic |
| AACACAACAACAACGAGCAT+TGG | 0.528793 | 5_1:-20666483 | Msa0723050:CDS |
| AAGTTGTGCCATTGTTCTGA+TGG | 0.534050 | 5_1:+20666686 | None:intergenic |
| ACAATCTTCACACATTTGCA+TGG | 0.536085 | 5_1:+20666094 | None:intergenic |
| GGAAGAACATCTTACTCCAT+TGG | 0.536644 | 5_1:-20668468 | Msa0723050:CDS |
| AAAAGTCGCGAAAGAAGAGT+TGG | 0.538902 | 5_1:-20666441 | Msa0723050:CDS |
| TCAGGTTGAAGACCTTGGTA+AGG | 0.540371 | 5_1:+20667827 | None:intergenic |
| CCATAAACTGATGATGAGCT+AGG | 0.545039 | 5_1:+20667869 | None:intergenic |
| GGGTGTGGAAGTAGCACTCC+TGG | 0.547961 | 5_1:+20666737 | None:intergenic |
| AAAGTTCAGGTTGAAGACCT+TGG | 0.548638 | 5_1:+20667822 | None:intergenic |
| GTGCTTATATAAAGGAGGAT+AGG | 0.553115 | 5_1:-20668640 | Msa0723050:five_prime_UTR |
| AAATTAATTTCTTGTCAGCA+TGG | 0.554884 | 5_1:-20667576 | Msa0723050:CDS |
| ACCTTCTCTAACAGCAATGA+GGG | 0.566133 | 5_1:+20667231 | None:intergenic |
| ACAGCAGCAGGAGCTAGCAT+TGG | 0.566143 | 5_1:+20665993 | None:intergenic |
| CACCCACCTAAATAAAATGA+GGG | 0.571153 | 5_1:-20668699 | None:intergenic |
| AGAAGATGAGTAACAGCCAA+TGG | 0.572438 | 5_1:+20668452 | None:intergenic |
| GTAGTGTATTGTAGTATGAA+GGG | 0.572719 | 5_1:-20666138 | Msa0723050:three_prime_UTR |
| AGGTATCTTTAATAATAGTA+GGG | 0.573912 | 5_1:+20668606 | None:intergenic |
| CTGCAGATCATAGTCACAAG+TGG | 0.577564 | 5_1:-20667624 | Msa0723050:CDS |
| CATCCATGAAAATTCTCAGT+GGG | 0.580339 | 5_1:-20668405 | Msa0723050:CDS |
| GAGAGTAGTAGTTCCATACC+AGG | 0.580651 | 5_1:-20666371 | Msa0723050:CDS |
| TATGCTGATGATGATGGGTG+TGG | 0.582762 | 5_1:+20666722 | None:intergenic |
| AATATAGGTGGGAAGGACAA+GGG | 0.583406 | 5_1:-20668135 | Msa0723050:intron |
| ACTGAGCTTCCCAAGTCCTA+GGG | 0.584163 | 5_1:+20667399 | None:intergenic |
| CACCTTCTCTAACAGCAATG+AGG | 0.591099 | 5_1:+20667230 | None:intergenic |
| ATGTTGATGATACCCTGGTA+TGG | 0.591188 | 5_1:+20666358 | None:intergenic |
| GCCCTCATTGCTGTTAGAGA+AGG | 0.593948 | 5_1:-20667232 | Msa0723050:CDS |
| ATATAGGTGGGAAGGACAAG+GGG | 0.594932 | 5_1:-20668134 | Msa0723050:intron |
| AGCATTGCACCACAGCATCA+AGG | 0.600171 | 5_1:-20666656 | Msa0723050:CDS |
| GGGGCATATGATAGATCAAG+AGG | 0.613030 | 5_1:-20668115 | Msa0723050:CDS |
| TGTTGTGGTGGAACAACTGA+AGG | 0.615956 | 5_1:+20666542 | None:intergenic |
| AGATCTATAACTATGGAGAA+GGG | 0.626888 | 5_1:-20667781 | Msa0723050:intron |
| TCAATTAGGAGCTGTTCACA+AGG | 0.628502 | 5_1:-20667203 | Msa0723050:intron |
| ATAGATCAAGAGGAAACAGA+AGG | 0.628702 | 5_1:-20668105 | Msa0723050:CDS |
| AAGGGTATGCTGATGATGAT+GGG | 0.632300 | 5_1:+20666717 | None:intergenic |
| AATTGATCACCTTGATGCTG+TGG | 0.645054 | 5_1:+20666647 | None:intergenic |
| AGACCTGAACAACTTGATGT+TGG | 0.656658 | 5_1:-20666395 | Msa0723050:CDS |
| TATATAAGCACACACCAACA+AGG | 0.657069 | 5_1:+20668652 | None:intergenic |
| GAATTAATTCAACCTTACCA+AGG | 0.657107 | 5_1:-20667839 | Msa0723050:CDS |
| ATGGTGGTCCATCAGAACAA+TGG | 0.666115 | 5_1:-20666694 | Msa0723050:CDS |
| GATTGAAGACTTGAGCTATG+TGG | 0.668398 | 5_1:-20666798 | Msa0723050:CDS |
| GGTGTGTGCTTATATAAAGG+AGG | 0.669304 | 5_1:-20668645 | Msa0723050:five_prime_UTR |
| TCATCAGCATACCCTTATGG+TGG | 0.673613 | 5_1:-20666710 | Msa0723050:CDS |
| AAATGATGTTGATGATACCC+TGG | 0.680740 | 5_1:+20666353 | None:intergenic |
| ACCCACCTAAATAAAATGAG+GGG | 0.695884 | 5_1:-20668698 | None:intergenic |
| GCAGCACCTGAAATAAACAC+TGG | 0.697600 | 5_1:-20667899 | Msa0723050:CDS |
| GCATCCATGAAAATTCTCAG+TGG | 0.703195 | 5_1:-20668406 | Msa0723050:CDS |
| AGAGTAGTAGTTCCATACCA+GGG | 0.724689 | 5_1:-20666370 | Msa0723050:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATAATTAATAATTAT+TGG | + | chr5_1:20668644-20668663 | None:intergenic | 0.0% |
| !!! | TATTAGTTAATTAGATTTTA+TGG | - | chr5_1:20666915-20666934 | Msa0723050:intron | 10.0% |
| !! | AATTGAATTAAATTAAAGAA+AGG | + | chr5_1:20667307-20667326 | None:intergenic | 10.0% |
| !!! | ATGTTTTTTGTGTATAATAT+AGG | - | chr5_1:20666434-20666453 | Msa0723050:CDS | 15.0% |
| !! | ACTTGATTATAAAAAAACAA+TGG | - | chr5_1:20666536-20666555 | Msa0723050:CDS | 15.0% |
| !! | AAATTAATTTCTTGATCATT+AGG | + | chr5_1:20666998-20667017 | None:intergenic | 15.0% |
| !! | AGGTATCTTTAATAATAGTA+GGG | + | chr5_1:20665981-20666000 | None:intergenic | 20.0% |
| !! | TAGGTATCTTTAATAATAGT+AGG | + | chr5_1:20665982-20666001 | None:intergenic | 20.0% |
| !! | GTTGCATATATATATATATG+AGG | - | chr5_1:20666345-20666364 | Msa0723050:CDS | 20.0% |
| !!! | TTTTTTGTGTATAATATAGG+TGG | - | chr5_1:20666437-20666456 | Msa0723050:CDS | 20.0% |
| !!! | TTTTTGTGTATAATATAGGT+GGG | - | chr5_1:20666438-20666457 | Msa0723050:CDS | 20.0% |
| !!! | AGTTTTAACTTTAAGCAAAT+AGG | - | chr5_1:20666890-20666909 | Msa0723050:intron | 20.0% |
| !!! | AAATAAAAGTATGTGAATGA+GGG | + | chr5_1:20667265-20667284 | None:intergenic | 20.0% |
| !!! | TAAATAAAAGTATGTGAATG+AGG | + | chr5_1:20667266-20667285 | None:intergenic | 20.0% |
| !! | CTGGTAATAATTTAAGAAAA+TGG | + | chr5_1:20667565-20667584 | None:intergenic | 20.0% |
| !! | CATAAAAAATCATGATATCA+TGG | - | chr5_1:20667649-20667668 | Msa0723050:intron | 20.0% |
| !! | AATGGATCAAACAATAATAA+TGG | - | chr5_1:20668273-20668292 | Msa0723050:intron | 20.0% |
| ! | TAAATGTGATATTTCCTTGT+TGG | - | chr5_1:20665918-20665937 | Msa0723050:three_prime_UTR | 25.0% |
| ! | TTAAAGATACCTATAGCTAA+AGG | - | chr5_1:20665989-20666008 | Msa0723050:three_prime_UTR | 25.0% |
| ! | TAAAGATACCTATAGCTAAA+GGG | - | chr5_1:20665990-20666009 | Msa0723050:three_prime_UTR | 25.0% |
| !! | TTAATTAGATTTTATGGAGC+AGG | - | chr5_1:20666921-20666940 | Msa0723050:intron | 25.0% |
| ! | AAATTAATTTCTTGTCAGCA+TGG | - | chr5_1:20667008-20667027 | Msa0723050:intron | 25.0% |
| ! | GAAATCATTCTATCTATTTC+AGG | + | chr5_1:20667155-20667174 | None:intergenic | 25.0% |
| ! | TTTGATTGAGTGAGTATAAT+TGG | + | chr5_1:20667742-20667761 | None:intergenic | 25.0% |
| !! | TCAATCAAATTTTGTCTCTA+TGG | - | chr5_1:20667753-20667772 | Msa0723050:intron | 25.0% |
| !! | TTTTGTCTCTATGGAATTTA+AGG | - | chr5_1:20667762-20667781 | Msa0723050:intron | 25.0% |
| !!! | GTTTGATTTTGAATTTGTTG+TGG | + | chr5_1:20668060-20668079 | None:intergenic | 25.0% |
| TTGCTATGATCTTCTTACTT+GGG | + | chr5_1:20666249-20666268 | None:intergenic | 30.0% | |
| ! | TTTGCTATGATCTTCTTACT+TGG | + | chr5_1:20666250-20666269 | None:intergenic | 30.0% |
| GAATTAATTCAACCTTACCA+AGG | - | chr5_1:20666745-20666764 | Msa0723050:CDS | 30.0% | |
| TCACATGAGATCTATAACTA+TGG | - | chr5_1:20666796-20666815 | Msa0723050:CDS | 30.0% | |
| AGATCTATAACTATGGAGAA+GGG | - | chr5_1:20666803-20666822 | Msa0723050:CDS | 30.0% | |
| ! | ACCATGTTTGAGATAACAAA+GGG | + | chr5_1:20667418-20667437 | None:intergenic | 30.0% |
| ! | AACCATGTTTGAGATAACAA+AGG | + | chr5_1:20667419-20667438 | None:intergenic | 30.0% |
| ! | TTTCAGATTTAAGACGTGTT+TGG | - | chr5_1:20667474-20667493 | Msa0723050:intron | 30.0% |
| TGTTAGACACAAACATAAGT+CGG | + | chr5_1:20667503-20667522 | None:intergenic | 30.0% | |
| !!! | GTGCAATGTTTTAAAGACAA+AGG | + | chr5_1:20667693-20667712 | None:intergenic | 30.0% |
| ! | ATTGATGGAGTTACTTTCAT+TGG | + | chr5_1:20667991-20668010 | None:intergenic | 30.0% |
| !!! | TGATTTTGAATTTGTTGTGG+TGG | + | chr5_1:20668057-20668076 | None:intergenic | 30.0% |
| ! | AACACAAACACAACATGTTT+TGG | - | chr5_1:20668077-20668096 | Msa0723050:intron | 30.0% |
| GTGAGAGAGTTATTAATGTT+AGG | - | chr5_1:20668364-20668383 | Msa0723050:CDS | 30.0% | |
| TGCATATTTCAGTTTCTTCT+TGG | - | chr5_1:20668424-20668443 | Msa0723050:CDS | 30.0% | |
| GTAGTGTATTGTAGTATGAA+GGG | - | chr5_1:20668446-20668465 | Msa0723050:CDS | 30.0% | |
| TATATAAGCACACACCAACA+AGG | + | chr5_1:20665935-20665954 | None:intergenic | 35.0% | |
| GTTGGTGTGTGCTTATATAA+AGG | - | chr5_1:20665936-20665955 | Msa0723050:three_prime_UTR | 35.0% | |
| GTGCTTATATAAAGGAGGAT+AGG | - | chr5_1:20665944-20665963 | Msa0723050:three_prime_UTR | 35.0% | |
| TCTTGAAACCCTTTAGCTAT+AGG | + | chr5_1:20666001-20666020 | None:intergenic | 35.0% | |
| CATCCATGAAAATTCTCAGT+GGG | - | chr5_1:20666179-20666198 | Msa0723050:three_prime_UTR | 35.0% | |
| ! | AAACCCACTGAGAATTTTCA+TGG | + | chr5_1:20666185-20666204 | None:intergenic | 35.0% |
| TGTGTATAATATAGGTGGGA+AGG | - | chr5_1:20666442-20666461 | Msa0723050:CDS | 35.0% | |
| ATAGATCAAGAGGAAACAGA+AGG | - | chr5_1:20666479-20666498 | Msa0723050:CDS | 35.0% | |
| TCTATGTTATATGCATGCAC+AGG | - | chr5_1:20666620-20666639 | Msa0723050:CDS | 35.0% | |
| TATATGCATGCACAGGATAT+TGG | - | chr5_1:20666627-20666646 | Msa0723050:CDS | 35.0% | |
| CAATCACCAGTGTTTATTTC+AGG | + | chr5_1:20666694-20666713 | None:intergenic | 35.0% | |
| CTAGCTCATCATCAGTTTAT+GGG | - | chr5_1:20666716-20666735 | Msa0723050:CDS | 35.0% | |
| GACATCTTGAAGAAAAGTTC+AGG | + | chr5_1:20666778-20666797 | None:intergenic | 35.0% | |
| GAGATCTATAACTATGGAGA+AGG | - | chr5_1:20666802-20666821 | Msa0723050:CDS | 35.0% | |
| !!! | ATTTTATGGAGCAGGTTGAT+TGG | - | chr5_1:20666929-20666948 | Msa0723050:intron | 35.0% |
| TCAGTTCTTGTCTGGTATAA+AGG | - | chr5_1:20667203-20667222 | Msa0723050:intron | 35.0% | |
| !! | AGAGAAGGTGTTGTTCAATT+AGG | - | chr5_1:20667367-20667386 | Msa0723050:intron | 35.0% |
| ! | TCCCTTTGTTATCTCAAACA+TGG | - | chr5_1:20667414-20667433 | Msa0723050:intron | 35.0% |
| ATGAAACACAACACTGATAC+TGG | + | chr5_1:20667584-20667603 | None:intergenic | 35.0% | |
| GTATCAGTGTTGTGTTTCAT+AGG | - | chr5_1:20667584-20667603 | Msa0723050:CDS | 35.0% | |
| GTTGGATAATGAAGAAGAAG+AGG | - | chr5_1:20668161-20668180 | Msa0723050:intron | 35.0% | |
| !! | AAATGATGTTGATGATACCC+TGG | + | chr5_1:20668234-20668253 | None:intergenic | 35.0% |
| CATTTCCATCAAGATCATCT+TGG | - | chr5_1:20668249-20668268 | Msa0723050:intron | 35.0% | |
| CATTTCCAAGATGATCTTGA+TGG | + | chr5_1:20668257-20668276 | None:intergenic | 35.0% | |
| CATCAAGATCATCTTGGAAA+TGG | - | chr5_1:20668255-20668274 | Msa0723050:intron | 35.0% | |
| CTCTCTCACTACAATATGTT+AGG | + | chr5_1:20668353-20668372 | None:intergenic | 35.0% | |
| GGTAGTGTATTGTAGTATGA+AGG | - | chr5_1:20668445-20668464 | Msa0723050:CDS | 35.0% | |
| CACACATTTGCATGGAATAA+TGG | + | chr5_1:20668485-20668504 | None:intergenic | 35.0% | |
| ACAATCTTCACACATTTGCA+TGG | + | chr5_1:20668493-20668512 | None:intergenic | 35.0% | |
| CCAAGTACAATGTAGTAGAT+TGG | + | chr5_1:20668563-20668582 | None:intergenic | 35.0% | |
| CCAATCTACTACATTGTACT+TGG | - | chr5_1:20668560-20668579 | Msa0723050:five_prime_UTR | 35.0% | |
| GGTGTGTGCTTATATAAAGG+AGG | - | chr5_1:20665939-20665958 | Msa0723050:three_prime_UTR | 40.0% | |
| GGAAGAACATCTTACTCCAT+TGG | - | chr5_1:20666116-20666135 | Msa0723050:three_prime_UTR | 40.0% | |
| AGAAGATGAGTAACAGCCAA+TGG | + | chr5_1:20666135-20666154 | None:intergenic | 40.0% | |
| GCATCCATGAAAATTCTCAG+TGG | - | chr5_1:20666178-20666197 | Msa0723050:three_prime_UTR | 40.0% | |
| !!! | AGTGGGTTTATGCTGTTTTC+TGG | - | chr5_1:20666196-20666215 | Msa0723050:three_prime_UTR | 40.0% |
| CTATGATCTTCTTACTTGGG+TGG | + | chr5_1:20666246-20666265 | None:intergenic | 40.0% | |
| TAATATAGGTGGGAAGGACA+AGG | - | chr5_1:20666448-20666467 | Msa0723050:CDS | 40.0% | |
| AATATAGGTGGGAAGGACAA+GGG | - | chr5_1:20666449-20666468 | Msa0723050:CDS | 40.0% | |
| CAAGAGGAAACAGAAGGAAT+TGG | - | chr5_1:20666485-20666504 | Msa0723050:CDS | 40.0% | |
| !! | CATGCACAGGATATTGGTTT+GGG | - | chr5_1:20666633-20666652 | Msa0723050:CDS | 40.0% |
| !! | AGGATATTGGTTTGGGAAGA+TGG | - | chr5_1:20666640-20666659 | Msa0723050:CDS | 40.0% |
| ! | CCATAAACTGATGATGAGCT+AGG | + | chr5_1:20666718-20666737 | None:intergenic | 40.0% |
| CCTAGCTCATCATCAGTTTA+TGG | - | chr5_1:20666715-20666734 | Msa0723050:CDS | 40.0% | |
| !! | AAAGTTCAGGTTGAAGACCT+TGG | + | chr5_1:20666765-20666784 | None:intergenic | 40.0% |
| ! | GATTTCTTTTACAGCACCCT+AGG | - | chr5_1:20667169-20667188 | Msa0723050:intron | 40.0% |
| ACCTTCTCTAACAGCAATGA+GGG | + | chr5_1:20667356-20667375 | None:intergenic | 40.0% | |
| TCAATTAGGAGCTGTTCACA+AGG | - | chr5_1:20667381-20667400 | Msa0723050:intron | 40.0% | |
| GATTGAAGACTTGAGCTATG+TGG | - | chr5_1:20667786-20667805 | Msa0723050:CDS | 40.0% | |
| ! | AGCTACATTGAAAGCATACC+AGG | - | chr5_1:20667829-20667848 | Msa0723050:CDS | 40.0% |
| ! | AAGGGTATGCTGATGATGAT+GGG | + | chr5_1:20667870-20667889 | None:intergenic | 40.0% |
| ! | TAAGGGTATGCTGATGATGA+TGG | + | chr5_1:20667871-20667890 | None:intergenic | 40.0% |
| TCATCATCAGCATACCCTTA+TGG | - | chr5_1:20667871-20667890 | Msa0723050:CDS | 40.0% | |
| ! | AAGTTGTGCCATTGTTCTGA+TGG | + | chr5_1:20667901-20667920 | None:intergenic | 40.0% |
| GAACAATGGCACAACTTTCA+AGG | - | chr5_1:20667904-20667923 | Msa0723050:CDS | 40.0% | |
| AATTGATCACCTTGATGCTG+TGG | + | chr5_1:20667940-20667959 | None:intergenic | 40.0% | |
| GCTTCAAGACTACTCATTGA+TGG | + | chr5_1:20668006-20668025 | None:intergenic | 40.0% | |
| AACACAACAACAACGAGCAT+TGG | - | chr5_1:20668101-20668120 | Msa0723050:CDS | 40.0% | |
| !! | CAACGAGCATTGGAATTTAC+AGG | - | chr5_1:20668111-20668130 | Msa0723050:CDS | 40.0% |
| AAAAGTCGCGAAAGAAGAGT+TGG | - | chr5_1:20668143-20668162 | Msa0723050:intron | 40.0% | |
| AGACCTGAACAACTTGATGT+TGG | - | chr5_1:20668189-20668208 | Msa0723050:intron | 40.0% | |
| ! | TCACCAACATCAAGTTGTTC+AGG | + | chr5_1:20668195-20668214 | None:intergenic | 40.0% |
| AGAGTAGTAGTTCCATACCA+GGG | - | chr5_1:20668214-20668233 | Msa0723050:intron | 40.0% | |
| !! | ATGTTGATGATACCCTGGTA+TGG | + | chr5_1:20668229-20668248 | None:intergenic | 40.0% |
| !!! | GGGTTTATGCTGTTTTCTGG+AGG | - | chr5_1:20666199-20666218 | Msa0723050:three_prime_UTR | 45.0% |
| GATCTTCTTACTTGGGTGGA+GGG | + | chr5_1:20666242-20666261 | None:intergenic | 45.0% | |
| TGATCTTCTTACTTGGGTGG+AGG | + | chr5_1:20666243-20666262 | None:intergenic | 45.0% | |
| ATATAGGTGGGAAGGACAAG+GGG | - | chr5_1:20666450-20666469 | Msa0723050:CDS | 45.0% | |
| GGGGCATATGATAGATCAAG+AGG | - | chr5_1:20666469-20666488 | Msa0723050:CDS | 45.0% | |
| ! | GCATGCACAGGATATTGGTT+TGG | - | chr5_1:20666632-20666651 | Msa0723050:CDS | 45.0% |
| GCAGCACCTGAAATAAACAC+TGG | - | chr5_1:20666685-20666704 | Msa0723050:CDS | 45.0% | |
| !! | TCAGGTTGAAGACCTTGGTA+AGG | + | chr5_1:20666760-20666779 | None:intergenic | 45.0% |
| CTGCAGATCATAGTCACAAG+TGG | - | chr5_1:20666960-20666979 | Msa0723050:intron | 45.0% | |
| ! | TTTTACAGCACCCTAGGACT+TGG | - | chr5_1:20667175-20667194 | Msa0723050:intron | 45.0% |
| TTTACAGCACCCTAGGACTT+GGG | - | chr5_1:20667176-20667195 | Msa0723050:intron | 45.0% | |
| CACCTTCTCTAACAGCAATG+AGG | + | chr5_1:20667357-20667376 | None:intergenic | 45.0% | |
| AACCGCAACCATGTTGCAAT+CGG | - | chr5_1:20667621-20667640 | Msa0723050:CDS | 45.0% | |
| ATGTCTCTCCGATTGCAACA+TGG | + | chr5_1:20667632-20667651 | None:intergenic | 45.0% | |
| TCATGGATCAGTCGCATTCT+CGG | - | chr5_1:20667666-20667685 | Msa0723050:intron | 45.0% | |
| !! | TATGCTGATGATGATGGGTG+TGG | + | chr5_1:20667865-20667884 | None:intergenic | 45.0% |
| TCATCAGCATACCCTTATGG+TGG | - | chr5_1:20667874-20667893 | Msa0723050:CDS | 45.0% | |
| ! | GTTCTGATGGACCACCATAA+GGG | + | chr5_1:20667888-20667907 | None:intergenic | 45.0% |
| ! | TGTTCTGATGGACCACCATA+AGG | + | chr5_1:20667889-20667908 | None:intergenic | 45.0% |
| ATGGTGGTCCATCAGAACAA+TGG | - | chr5_1:20667890-20667909 | Msa0723050:CDS | 45.0% | |
| ! | TGTTGTGGTGGAACAACTGA+AGG | + | chr5_1:20668045-20668064 | None:intergenic | 45.0% |
| GAGAGTAGTAGTTCCATACC+AGG | - | chr5_1:20668213-20668232 | Msa0723050:intron | 45.0% | |
| TTGCATCCTTAAACAGCAGC+AGG | + | chr5_1:20668606-20668625 | None:intergenic | 45.0% | |
| !!! | TAAGAATTTTATTTTATATA+TGG | - | chr5_1:20666095-20666114 | Msa0723050:three_prime_UTR | 5.0% |
| TTGGGTGGAGGGTAGTTTCT+AGG | + | chr5_1:20666231-20666250 | None:intergenic | 50.0% | |
| ACTGAGCTTCCCAAGTCCTA+GGG | + | chr5_1:20667188-20667207 | None:intergenic | 50.0% | |
| AACTGAGCTTCCCAAGTCCT+AGG | + | chr5_1:20667189-20667208 | None:intergenic | 50.0% | |
| TGGGAAGCTCAGTTCTTGTC+TGG | - | chr5_1:20667195-20667214 | Msa0723050:intron | 50.0% | |
| ! | GCCCTCATTGCTGTTAGAGA+AGG | - | chr5_1:20667352-20667371 | Msa0723050:intron | 50.0% |
| CTCCGATTGCAACATGGTTG+CGG | + | chr5_1:20667626-20667645 | None:intergenic | 50.0% | |
| AGCATTGCACCACAGCATCA+AGG | - | chr5_1:20667928-20667947 | Msa0723050:CDS | 50.0% | |
| ! | CTAGCTCCTGCTGCTGTTTA+AGG | - | chr5_1:20668597-20668616 | Msa0723050:five_prime_UTR | 50.0% |
| ACAGCAGCAGGAGCTAGCAT+TGG | + | chr5_1:20668594-20668613 | None:intergenic | 55.0% | |
| GGGTGTGGAAGTAGCACTCC+TGG | + | chr5_1:20667850-20667869 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 20665900 | 20668706 | 20665900 | ID=Msa0723050;Name=Msa0723050 |
| chr5_1 | mRNA | 20665900 | 20668706 | 20665900 | ID=Msa0723050-mRNA-1;Parent=Msa0723050;Name=Msa0723050-mRNA-1;_AED=0.16;_eAED=0.16;_QI=214|1|1|1|1|1|7|404|364 |
| chr5_1 | exon | 20665900 | 20666822 | 20665900 | ID=Msa0723050-mRNA-1:exon:7880;Parent=Msa0723050-mRNA-1 |
| chr5_1 | exon | 20667204 | 20667260 | 20667204 | ID=Msa0723050-mRNA-1:exon:7879;Parent=Msa0723050-mRNA-1 |
| chr5_1 | exon | 20667382 | 20667423 | 20667382 | ID=Msa0723050-mRNA-1:exon:7878;Parent=Msa0723050-mRNA-1 |
| chr5_1 | exon | 20667558 | 20667663 | 20667558 | ID=Msa0723050-mRNA-1:exon:7877;Parent=Msa0723050-mRNA-1 |
| chr5_1 | exon | 20667782 | 20667964 | 20667782 | ID=Msa0723050-mRNA-1:exon:7876;Parent=Msa0723050-mRNA-1 |
| chr5_1 | exon | 20668100 | 20668150 | 20668100 | ID=Msa0723050-mRNA-1:exon:7875;Parent=Msa0723050-mRNA-1 |
| chr5_1 | exon | 20668356 | 20668706 | 20668356 | ID=Msa0723050-mRNA-1:exon:7874;Parent=Msa0723050-mRNA-1 |
| chr5_1 | five_prime_UTR | 20668493 | 20668706 | 20668493 | ID=Msa0723050-mRNA-1:five_prime_utr;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20668356 | 20668492 | 20668356 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20668100 | 20668150 | 20668100 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20667782 | 20667964 | 20667782 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20667558 | 20667663 | 20667558 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20667382 | 20667423 | 20667382 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20667204 | 20667260 | 20667204 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | CDS | 20666304 | 20666822 | 20666304 | ID=Msa0723050-mRNA-1:cds;Parent=Msa0723050-mRNA-1 |
| chr5_1 | three_prime_UTR | 20665900 | 20666303 | 20665900 | ID=Msa0723050-mRNA-1:three_prime_utr;Parent=Msa0723050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
