Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0722780 | XP_003613278.1 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0722780 | sp|K4PW38|RSS3_ORYSJ | 36.444 | 225 | 105 | 7 | 14 | 227 | 31 | 228 | 8.58e-36 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0722780 | G7K2R0 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000190 | Msa0722780 | 0.819476 | 1.138682e-52 | -8.615850e-47 |
| Msa0017510 | Msa0722780 | 0.828588 | 8.270250e-55 | -8.615850e-47 |
| Msa0029890 | Msa0722780 | 0.808628 | 2.820306e-50 | -8.615850e-47 |
| Msa0101810 | Msa0722780 | -0.822806 | 1.944952e-53 | -8.615850e-47 |
| Msa0110970 | Msa0722780 | 0.818757 | 1.659283e-52 | -8.615850e-47 |
| Msa0114540 | Msa0722780 | -0.810135 | 1.339937e-50 | -8.615850e-47 |
| Msa0682620 | Msa0722780 | 0.812082 | 5.068827e-51 | -8.615850e-47 |
| Msa0688770 | Msa0722780 | 0.818534 | 1.864121e-52 | -8.615850e-47 |
| Msa0719490 | Msa0722780 | -0.816894 | 4.369199e-52 | -8.615850e-47 |
| Msa0722780 | Msa0722850 | 0.928488 | 2.690675e-92 | -8.615850e-47 |
| Msa0722780 | Msa0723000 | 0.967776 | 9.562894e-128 | -8.615850e-47 |
| Msa0722780 | Msa0723050 | 0.966226 | 1.224203e-125 | -8.615850e-47 |
| Msa0722780 | Msa0756630 | -0.826882 | 2.125279e-54 | -8.615850e-47 |
| Msa0722780 | Msa0802470 | -0.827321 | 1.668436e-54 | -8.615850e-47 |
| Msa0722780 | Msa0828560 | 0.806656 | 7.398756e-50 | -8.615850e-47 |
| Msa0722780 | Msa0843220 | 0.917633 | 4.186978e-86 | -8.615850e-47 |
| Msa0722780 | Msa0843780 | -0.800518 | 1.388230e-48 | -8.615850e-47 |
| Msa0722780 | Msa0867890 | 0.803622 | 3.193137e-49 | -8.615850e-47 |
| Msa0722780 | Msa0880140 | 0.830258 | 3.248871e-55 | -8.615850e-47 |
| Msa0722780 | Msa0896970 | 0.819469 | 1.142468e-52 | -8.615850e-47 |
| Msa0722780 | Msa0908050 | 0.806434 | 8.243705e-50 | -8.615850e-47 |
| Msa0722780 | Msa0930780 | 0.811441 | 6.990087e-51 | -8.615850e-47 |
| Msa0722780 | Msa0967380 | 0.806357 | 8.556436e-50 | -8.615850e-47 |
| Msa0722780 | Msa0996960 | 0.806519 | 7.909722e-50 | -8.615850e-47 |
| Msa0722780 | Msa1009000 | 0.817351 | 3.449899e-52 | -8.615850e-47 |
| Msa0722780 | Msa1075220 | -0.823978 | 1.035313e-53 | -8.615850e-47 |
| Msa0722780 | Msa1077180 | 0.836725 | 7.906216e-57 | -8.615850e-47 |
| Msa0722780 | Msa1083410 | 0.809721 | 1.644977e-50 | -8.615850e-47 |
| Msa0722780 | Msa1098560 | 0.834429 | 3.013486e-56 | -8.615850e-47 |
| Msa0722780 | Msa1116130 | -0.805529 | 1.277214e-49 | -8.615850e-47 |
| Msa0722780 | Msa1124680 | -0.813786 | 2.146154e-51 | -8.615850e-47 |
| Msa0722780 | Msa1128160 | 0.801348 | 9.394282e-49 | -8.615850e-47 |
| Msa0722780 | Msa1162550 | -0.805929 | 1.052663e-49 | -8.615850e-47 |
| Msa0722780 | Msa1169710 | 0.807984 | 3.868964e-50 | -8.615850e-47 |
| Msa0722780 | Msa1222970 | 0.839040 | 2.009306e-57 | -8.615850e-47 |
| Msa0722780 | Msa1228210 | 0.815050 | 1.127727e-51 | -8.615850e-47 |
| Msa0722780 | Msa1242220 | 0.813908 | 2.017347e-51 | -8.615850e-47 |
| Msa0722780 | Msa1242230 | 0.824597 | 7.404020e-54 | -8.615850e-47 |
| Msa0722780 | Msa1242240 | 0.805788 | 1.127036e-49 | -8.615850e-47 |
| Msa0722780 | Msa1264020 | 0.818948 | 1.501634e-52 | -8.615850e-47 |
| Msa0722780 | Msa1271280 | 0.812652 | 3.806108e-51 | -8.615850e-47 |
| Msa0722780 | Msa1278840 | 0.809953 | 1.466170e-50 | -8.615850e-47 |
| Msa0722780 | Msa1283840 | 0.809647 | 1.706187e-50 | -8.615850e-47 |
| Msa0722780 | Msa1283860 | 0.800571 | 1.353760e-48 | -8.615850e-47 |
| Msa0722780 | Msa1284260 | 0.826604 | 2.476237e-54 | -8.615850e-47 |
| Msa0722780 | Msa1293770 | 0.824379 | 8.334180e-54 | -8.615850e-47 |
| Msa0722780 | Msa1322860 | 0.831875 | 1.302236e-55 | -8.615850e-47 |
| Msa0722780 | Msa1322890 | 0.816960 | 4.223403e-52 | -8.615850e-47 |
| Msa0722780 | Msa1324500 | 0.804481 | 2.116232e-49 | -8.615850e-47 |
| Msa0722780 | Msa1327050 | 0.825152 | 5.476280e-54 | -8.615850e-47 |
| Msa0722780 | Msa1367080 | 0.827829 | 1.260227e-54 | -8.615850e-47 |
| Msa0722780 | Msa1374550 | 0.859233 | 4.766292e-63 | -8.615850e-47 |
| Msa0125090 | Msa0722780 | 0.807019 | 6.200090e-50 | -8.615850e-47 |
| Msa0125100 | Msa0722780 | 0.808876 | 2.496256e-50 | -8.615850e-47 |
| Msa0140780 | Msa0722780 | 0.807786 | 4.263176e-50 | -8.615850e-47 |
| Msa0157290 | Msa0722780 | 0.828853 | 7.134809e-55 | -8.615850e-47 |
| Msa0169860 | Msa0722780 | 0.814836 | 1.257806e-51 | -8.615850e-47 |
| Msa0197230 | Msa0722780 | 0.810518 | 1.107571e-50 | -8.615850e-47 |
| Msa0202950 | Msa0722780 | 0.825829 | 3.786599e-54 | -8.615850e-47 |
| Msa0203010 | Msa0722780 | 0.806280 | 8.882807e-50 | -8.615850e-47 |
| Msa0221290 | Msa0722780 | 0.808534 | 2.954868e-50 | -8.615850e-47 |
| Msa0385390 | Msa0722780 | 0.816036 | 6.802945e-52 | -8.615850e-47 |
| Msa0392420 | Msa0722780 | 0.834159 | 3.522126e-56 | -8.615850e-47 |
| Msa0392460 | Msa0722780 | 0.834035 | 3.781348e-56 | -8.615850e-47 |
| Msa0392490 | Msa0722780 | 0.832665 | 8.303448e-56 | -8.615850e-47 |
| Msa0395790 | Msa0722780 | 0.805193 | 1.502518e-49 | -8.615850e-47 |
| Msa0415320 | Msa0722780 | 0.802617 | 5.152651e-49 | -8.615850e-47 |
| Msa0465910 | Msa0722780 | 0.811935 | 5.458366e-51 | -8.615850e-47 |
| Msa0465950 | Msa0722780 | 0.838059 | 3.600311e-57 | -8.615850e-47 |
| Msa0480980 | Msa0722780 | 0.823520 | 1.324945e-53 | -8.615850e-47 |
| Msa0243100 | Msa0722780 | 0.811543 | 6.642900e-51 | -8.615850e-47 |
| Msa0261340 | Msa0722780 | 0.807398 | 5.153430e-50 | -8.615850e-47 |
| Msa0284420 | Msa0722780 | 0.822901 | 1.848256e-53 | -8.615850e-47 |
| Msa0287650 | Msa0722780 | 0.810320 | 1.222446e-50 | -8.615850e-47 |
| Msa0344570 | Msa0722780 | 0.815798 | 7.686654e-52 | -8.615850e-47 |
| Msa0487570 | Msa0722780 | 0.833168 | 6.226622e-56 | -8.615850e-47 |
| Msa0487630 | Msa0722780 | 0.837552 | 4.859541e-57 | -8.615850e-47 |
| Msa0535040 | Msa0722780 | 0.819066 | 1.411883e-52 | -8.615850e-47 |
| Msa0548280 | Msa0722780 | -0.815697 | 8.093232e-52 | -8.615850e-47 |
| Msa0574890 | Msa0722780 | 0.803405 | 3.541786e-49 | -8.615850e-47 |
| Msa0581760 | Msa0722780 | 0.828606 | 8.187350e-55 | -8.615850e-47 |
| Msa0591790 | Msa0722780 | 0.806982 | 6.312797e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0722780 | MtrunA17_Chr5g0413871 | 97.802 | 364 | 6 | 1 | 1 | 364 | 1 | 362 | 0.0 | 739 |
| Msa0722780 | MtrunA17_Chr1g0152981 | 51.185 | 211 | 80 | 5 | 13 | 217 | 11 | 204 | 6.14e-70 | 221 |
| Msa0722780 | MtrunA17_Chr2g0317011 | 38.537 | 205 | 99 | 6 | 14 | 218 | 20 | 197 | 5.12e-36 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0722780 | AT1G60060.1 | 68.681 | 364 | 65 | 10 | 1 | 336 | 1 | 343 | 4.00e-160 | 454 |
| Msa0722780 | AT1G60060.2 | 64.263 | 319 | 65 | 10 | 46 | 336 | 6 | 303 | 1.12e-123 | 360 |
| Msa0722780 | AT5G53900.2 | 38.333 | 240 | 118 | 7 | 14 | 244 | 21 | 239 | 8.39e-39 | 142 |
| Msa0722780 | AT3G15240.2 | 37.073 | 205 | 107 | 4 | 14 | 218 | 34 | 216 | 9.58e-38 | 139 |
| Msa0722780 | AT5G53900.3 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.58e-20 | 89.7 |
| Msa0722780 | AT5G53900.1 | 39.844 | 128 | 67 | 2 | 126 | 244 | 1 | 127 | 2.58e-20 | 89.7 |
| Msa0722780 | AT2G31280.1 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 1.83e-14 | 75.1 |
| Msa0722780 | AT2G31280.3 | 30.928 | 194 | 83 | 9 | 15 | 207 | 6 | 149 | 2.08e-14 | 75.1 |
| Msa0722780 | AT2G27230.2 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 2.79e-13 | 71.6 |
| Msa0722780 | AT2G27230.1 | 27.488 | 211 | 105 | 8 | 13 | 218 | 4 | 171 | 2.79e-13 | 71.6 |
Find 102 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGCTCCTGCTGCTGTTTA+AGG | 0.126120 | 5_1:+20253428 | Msa0722780:three_prime_UTR |
| CCTAGCTCATCATCAGTTTA+TGG | 0.128324 | 5_1:+20251546 | Msa0722780:CDS |
| ATAAAATAATTAATAATTAT+TGG | 0.163550 | 5_1:-20253472 | None:intergenic |
| CAATCACCAGTGTTTATTTC+AGG | 0.239507 | 5_1:-20251522 | None:intergenic |
| TGCATATTTCAGTTTCTTCT+TGG | 0.255252 | 5_1:+20253255 | Msa0722780:three_prime_UTR |
| TCACATGAGATCTATAACTA+TGG | 0.263874 | 5_1:+20251627 | Msa0722780:CDS |
| CACACATTTGCATGGAATAA+TGG | 0.268865 | 5_1:-20253313 | None:intergenic |
| TTGGGTGGAGGGTAGTTTCT+AGG | 0.275012 | 5_1:-20251059 | None:intergenic |
| AACACAAACACAACATGTTT+TGG | 0.280192 | 5_1:+20252908 | Msa0722780:CDS |
| AGAGAAGGTGTTGTTCAATT+AGG | 0.283607 | 5_1:+20252198 | Msa0722780:CDS |
| AAATTAATTTCTTGATCATT+AGG | 0.297602 | 5_1:-20251826 | None:intergenic |
| CAACGAGCATTGGAATTTAC+AGG | 0.318102 | 5_1:+20252942 | Msa0722780:CDS |
| TATATGCATGCACAGGATAT+TGG | 0.324411 | 5_1:+20251458 | Msa0722780:intron |
| CATTTCCAAGATGATCTTGA+TGG | 0.328838 | 5_1:-20253085 | None:intergenic |
| AGGATATTGGTTTGGGAAGA+TGG | 0.329021 | 5_1:+20251471 | Msa0722780:intron |
| TCTTGAAACCCTTTAGCTAT+AGG | 0.329720 | 5_1:-20250828 | None:intergenic |
| ATTGATGGAGTTACTTTCAT+TGG | 0.336300 | 5_1:-20252819 | None:intergenic |
| AATGGATCAAACAATAATAA+TGG | 0.339598 | 5_1:+20253104 | Msa0722780:CDS |
| CATCAAGATCATCTTGGAAA+TGG | 0.346285 | 5_1:+20253086 | Msa0722780:CDS |
| TCAGTTCTTGTCTGGTATAA+AGG | 0.362612 | 5_1:+20252034 | Msa0722780:CDS |
| GCATGCACAGGATATTGGTT+TGG | 0.384339 | 5_1:+20251463 | Msa0722780:intron |
| GTGAGAGAGTTATTAATGTT+AGG | 0.386113 | 5_1:+20253195 | Msa0722780:three_prime_UTR |
| TCACCAACATCAAGTTGTTC+AGG | 0.392523 | 5_1:-20253023 | None:intergenic |
| GACATCTTGAAGAAAAGTTC+AGG | 0.396788 | 5_1:-20251606 | None:intergenic |
| CATGCACAGGATATTGGTTT+GGG | 0.400265 | 5_1:+20251464 | Msa0722780:intron |
| TCATCATCAGCATACCCTTA+TGG | 0.403770 | 5_1:+20252702 | Msa0722780:CDS |
| TAAAGATACCTATAGCTAAA+GGG | 0.404162 | 5_1:+20250820 | Msa0722780:five_prime_UTR |
| GTTGGTGTGTGCTTATATAA+AGG | 0.405928 | 5_1:+20250766 | Msa0722780:five_prime_UTR |
| GCTTCAAGACTACTCATTGA+TGG | 0.406362 | 5_1:-20252834 | None:intergenic |
| TTAAAGATACCTATAGCTAA+AGG | 0.409220 | 5_1:+20250819 | Msa0722780:five_prime_UTR |
| TGGGAAGCTCAGTTCTTGTC+TGG | 0.414012 | 5_1:+20252026 | Msa0722780:CDS |
| CTAGCTCATCATCAGTTTAT+GGG | 0.418675 | 5_1:+20251547 | Msa0722780:CDS |
| CTCTCTCACTACAATATGTT+AGG | 0.427654 | 5_1:-20253181 | None:intergenic |
| TAGGTATCTTTAATAATAGT+AGG | 0.427738 | 5_1:-20250809 | None:intergenic |
| CCAATCTACTACATTGTACT+TGG | 0.431351 | 5_1:+20253391 | Msa0722780:three_prime_UTR |
| TAAATGTGATATTTCCTTGT+TGG | 0.431856 | 5_1:+20250748 | Msa0722780:five_prime_UTR |
| CATTTCCATCAAGATCATCT+TGG | 0.434289 | 5_1:+20253080 | Msa0722780:CDS |
| TTTACAGCACCCTAGGACTT+GGG | 0.440440 | 5_1:+20252007 | Msa0722780:intron |
| CAAGAGGAAACAGAAGGAAT+TGG | 0.444449 | 5_1:+20251316 | Msa0722780:CDS |
| TGTTCTGATGGACCACCATA+AGG | 0.450763 | 5_1:-20252717 | None:intergenic |
| GGTAGTGTATTGTAGTATGA+AGG | 0.452877 | 5_1:+20253276 | Msa0722780:three_prime_UTR |
| CTATGATCTTCTTACTTGGG+TGG | 0.478789 | 5_1:-20251074 | None:intergenic |
| TGATCTTCTTACTTGGGTGG+AGG | 0.479359 | 5_1:-20251071 | None:intergenic |
| GAACAATGGCACAACTTTCA+AGG | 0.484632 | 5_1:+20252735 | Msa0722780:CDS |
| GTTGGATAATGAAGAAGAAG+AGG | 0.487343 | 5_1:+20252992 | Msa0722780:CDS |
| CCAAGTACAATGTAGTAGAT+TGG | 0.489579 | 5_1:-20253391 | None:intergenic |
| AACTGAGCTTCCCAAGTCCT+AGG | 0.490005 | 5_1:-20252017 | None:intergenic |
| AGCTACATTGAAAGCATACC+AGG | 0.501005 | 5_1:+20252660 | Msa0722780:CDS |
| GTTCTGATGGACCACCATAA+GGG | 0.509808 | 5_1:-20252716 | None:intergenic |
| GAGATCTATAACTATGGAGA+AGG | 0.511952 | 5_1:+20251633 | Msa0722780:CDS |
| TAATATAGGTGGGAAGGACA+AGG | 0.515798 | 5_1:+20251279 | Msa0722780:intron |
| TAAGGGTATGCTGATGATGA+TGG | 0.520173 | 5_1:-20252699 | None:intergenic |
| GATCTTCTTACTTGGGTGGA+GGG | 0.521140 | 5_1:-20251070 | None:intergenic |
| TGTGTATAATATAGGTGGGA+AGG | 0.521340 | 5_1:+20251273 | Msa0722780:intron |
| TTGCATCCTTAAACAGCAGC+AGG | 0.525427 | 5_1:-20253434 | None:intergenic |
| AACACAACAACAACGAGCAT+TGG | 0.528793 | 5_1:+20252932 | Msa0722780:CDS |
| AAGTTGTGCCATTGTTCTGA+TGG | 0.534050 | 5_1:-20252729 | None:intergenic |
| ACAATCTTCACACATTTGCA+TGG | 0.536085 | 5_1:-20253321 | None:intergenic |
| GGAAGAACATCTTACTCCAT+TGG | 0.536644 | 5_1:+20250947 | Msa0722780:CDS |
| AAAAGTCGCGAAAGAAGAGT+TGG | 0.538902 | 5_1:+20252974 | Msa0722780:CDS |
| TCAGGTTGAAGACCTTGGTA+AGG | 0.540371 | 5_1:-20251588 | None:intergenic |
| CCATAAACTGATGATGAGCT+AGG | 0.545039 | 5_1:-20251546 | None:intergenic |
| GGGTGTGGAAGTAGCACTCC+TGG | 0.547961 | 5_1:-20252678 | None:intergenic |
| AAAGTTCAGGTTGAAGACCT+TGG | 0.548638 | 5_1:-20251593 | None:intergenic |
| GTGCTTATATAAAGGAGGAT+AGG | 0.553115 | 5_1:+20250774 | Msa0722780:five_prime_UTR |
| AAATTAATTTCTTGTCAGCA+TGG | 0.554884 | 5_1:+20251839 | Msa0722780:CDS |
| ACCTTCTCTAACAGCAATGA+GGG | 0.566133 | 5_1:-20252184 | None:intergenic |
| ACAGCAGCAGGAGCTAGCAT+TGG | 0.566143 | 5_1:-20253422 | None:intergenic |
| CACCCACCTAAATAAAATGA+GGG | 0.571153 | 5_1:+20250715 | None:intergenic |
| AGAAGATGAGTAACAGCCAA+TGG | 0.572438 | 5_1:-20250963 | None:intergenic |
| GTAGTGTATTGTAGTATGAA+GGG | 0.572719 | 5_1:+20253277 | Msa0722780:three_prime_UTR |
| AGGTATCTTTAATAATAGTA+GGG | 0.573912 | 5_1:-20250808 | None:intergenic |
| CTGCAGATCATAGTCACAAG+TGG | 0.577564 | 5_1:+20251791 | Msa0722780:CDS |
| CATCCATGAAAATTCTCAGT+GGG | 0.580339 | 5_1:+20251010 | Msa0722780:CDS |
| GAGAGTAGTAGTTCCATACC+AGG | 0.580651 | 5_1:+20253044 | Msa0722780:CDS |
| TATGCTGATGATGATGGGTG+TGG | 0.582762 | 5_1:-20252693 | None:intergenic |
| AATATAGGTGGGAAGGACAA+GGG | 0.583406 | 5_1:+20251280 | Msa0722780:intron |
| ACTGAGCTTCCCAAGTCCTA+GGG | 0.584163 | 5_1:-20252016 | None:intergenic |
| CACCTTCTCTAACAGCAATG+AGG | 0.591099 | 5_1:-20252185 | None:intergenic |
| ATGTTGATGATACCCTGGTA+TGG | 0.591188 | 5_1:-20253057 | None:intergenic |
| GCCCTCATTGCTGTTAGAGA+AGG | 0.593948 | 5_1:+20252183 | Msa0722780:CDS |
| ATATAGGTGGGAAGGACAAG+GGG | 0.594932 | 5_1:+20251281 | Msa0722780:intron |
| AGCATTGCACCACAGCATCA+AGG | 0.600171 | 5_1:+20252759 | Msa0722780:CDS |
| GGGGCATATGATAGATCAAG+AGG | 0.613030 | 5_1:+20251300 | Msa0722780:CDS |
| TGTTGTGGTGGAACAACTGA+AGG | 0.615956 | 5_1:-20252873 | None:intergenic |
| AGATCTATAACTATGGAGAA+GGG | 0.626888 | 5_1:+20251634 | Msa0722780:CDS |
| TCAATTAGGAGCTGTTCACA+AGG | 0.628502 | 5_1:+20252212 | Msa0722780:CDS |
| ATAGATCAAGAGGAAACAGA+AGG | 0.628702 | 5_1:+20251310 | Msa0722780:CDS |
| AAGGGTATGCTGATGATGAT+GGG | 0.632300 | 5_1:-20252698 | None:intergenic |
| AATTGATCACCTTGATGCTG+TGG | 0.645054 | 5_1:-20252768 | None:intergenic |
| AGACCTGAACAACTTGATGT+TGG | 0.656658 | 5_1:+20253020 | Msa0722780:CDS |
| TATATAAGCACACACCAACA+AGG | 0.657069 | 5_1:-20250762 | None:intergenic |
| GAATTAATTCAACCTTACCA+AGG | 0.657107 | 5_1:+20251576 | Msa0722780:CDS |
| ATGGTGGTCCATCAGAACAA+TGG | 0.666115 | 5_1:+20252721 | Msa0722780:CDS |
| GATTGAAGACTTGAGCTATG+TGG | 0.668398 | 5_1:+20252617 | Msa0722780:CDS |
| GGTGTGTGCTTATATAAAGG+AGG | 0.669304 | 5_1:+20250769 | Msa0722780:five_prime_UTR |
| TCATCAGCATACCCTTATGG+TGG | 0.673613 | 5_1:+20252705 | Msa0722780:CDS |
| AAATGATGTTGATGATACCC+TGG | 0.680740 | 5_1:-20253062 | None:intergenic |
| ACCCACCTAAATAAAATGAG+GGG | 0.695884 | 5_1:+20250716 | None:intergenic |
| GCAGCACCTGAAATAAACAC+TGG | 0.697600 | 5_1:+20251516 | Msa0722780:CDS |
| GCATCCATGAAAATTCTCAG+TGG | 0.703195 | 5_1:+20251009 | Msa0722780:CDS |
| AGAGTAGTAGTTCCATACCA+GGG | 0.724689 | 5_1:+20253045 | Msa0722780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATAATTAATAATTAT+TGG | - | chr5_1:20253475-20253494 | None:intergenic | 0.0% |
| !! | ACTTGATTATAAAAAAAAAA+TGG | + | chr5_1:20251367-20251386 | Msa0722780:intron | 10.0% |
| !!! | TATTAGTTAATTAGATTTTA+TGG | + | chr5_1:20251746-20251765 | Msa0722780:intron | 10.0% |
| !! | AATTGAATTAAATTAAAGAA+AGG | - | chr5_1:20252138-20252157 | None:intergenic | 10.0% |
| !!! | ATGTTTTTTGTGTATAATAT+AGG | + | chr5_1:20251265-20251284 | Msa0722780:intron | 15.0% |
| !! | AAATTAATTTCTTGATCATT+AGG | - | chr5_1:20251829-20251848 | None:intergenic | 15.0% |
| !! | AGGTATCTTTAATAATAGTA+GGG | - | chr5_1:20250811-20250830 | None:intergenic | 20.0% |
| !! | TAGGTATCTTTAATAATAGT+AGG | - | chr5_1:20250812-20250831 | None:intergenic | 20.0% |
| !! | GTTGCATATATATATATATG+AGG | + | chr5_1:20251176-20251195 | Msa0722780:intron | 20.0% |
| !!! | TTTTTTGTGTATAATATAGG+TGG | + | chr5_1:20251268-20251287 | Msa0722780:intron | 20.0% |
| !!! | TTTTTGTGTATAATATAGGT+GGG | + | chr5_1:20251269-20251288 | Msa0722780:intron | 20.0% |
| !!! | AGTTTTAACTTTAAGCAAAT+AGG | + | chr5_1:20251721-20251740 | Msa0722780:intron | 20.0% |
| !!! | AAATAAAAGTATGTGAATGA+GGG | - | chr5_1:20252096-20252115 | None:intergenic | 20.0% |
| !!! | TAAATAAAAGTATGTGAATG+AGG | - | chr5_1:20252097-20252116 | None:intergenic | 20.0% |
| !! | CTGGTAATAATTTAAGAAAA+TGG | - | chr5_1:20252396-20252415 | None:intergenic | 20.0% |
| !! | CATAAAAAATCATGATATCA+TGG | + | chr5_1:20252480-20252499 | Msa0722780:intron | 20.0% |
| !! | AATGGATCAAACAATAATAA+TGG | + | chr5_1:20253104-20253123 | Msa0722780:CDS | 20.0% |
| ! | TAAATGTGATATTTCCTTGT+TGG | + | chr5_1:20250748-20250767 | Msa0722780:five_prime_UTR | 25.0% |
| ! | TTAAAGATACCTATAGCTAA+AGG | + | chr5_1:20250819-20250838 | Msa0722780:five_prime_UTR | 25.0% |
| ! | TAAAGATACCTATAGCTAAA+GGG | + | chr5_1:20250820-20250839 | Msa0722780:five_prime_UTR | 25.0% |
| !! | TTAATTAGATTTTATGGAGC+AGG | + | chr5_1:20251752-20251771 | Msa0722780:intron | 25.0% |
| ! | AAATTAATTTCTTGTCAGCA+TGG | + | chr5_1:20251839-20251858 | Msa0722780:CDS | 25.0% |
| ! | GAAATCATTCTATCTATTTC+AGG | - | chr5_1:20251986-20252005 | None:intergenic | 25.0% |
| ! | TTTGATTGAGTGAGTATAAT+TGG | - | chr5_1:20252573-20252592 | None:intergenic | 25.0% |
| !! | TCAATCAAATTTTGTCTCTA+TGG | + | chr5_1:20252584-20252603 | Msa0722780:intron | 25.0% |
| !! | TTTTGTCTCTATGGAATTTA+AGG | + | chr5_1:20252593-20252612 | Msa0722780:intron | 25.0% |
| !!! | GTTTGATTTTGAATTTGTTG+TGG | - | chr5_1:20252891-20252910 | None:intergenic | 25.0% |
| TTGCTATGATCTTCTTACTT+GGG | - | chr5_1:20251080-20251099 | None:intergenic | 30.0% | |
| ! | TTTGCTATGATCTTCTTACT+TGG | - | chr5_1:20251081-20251100 | None:intergenic | 30.0% |
| GAATTAATTCAACCTTACCA+AGG | + | chr5_1:20251576-20251595 | Msa0722780:CDS | 30.0% | |
| TCACATGAGATCTATAACTA+TGG | + | chr5_1:20251627-20251646 | Msa0722780:CDS | 30.0% | |
| AGATCTATAACTATGGAGAA+GGG | + | chr5_1:20251634-20251653 | Msa0722780:CDS | 30.0% | |
| ! | ACCATGTTTGAGATAACAAA+GGG | - | chr5_1:20252249-20252268 | None:intergenic | 30.0% |
| ! | AACCATGTTTGAGATAACAA+AGG | - | chr5_1:20252250-20252269 | None:intergenic | 30.0% |
| ! | TTTCAGATTTAAGACGTGTT+TGG | + | chr5_1:20252305-20252324 | Msa0722780:intron | 30.0% |
| TGTTAGACACAAACATAAGT+CGG | - | chr5_1:20252334-20252353 | None:intergenic | 30.0% | |
| !!! | GTGCAATGTTTTAAAGACAA+AGG | - | chr5_1:20252524-20252543 | None:intergenic | 30.0% |
| ! | ATTGATGGAGTTACTTTCAT+TGG | - | chr5_1:20252822-20252841 | None:intergenic | 30.0% |
| !!! | TGATTTTGAATTTGTTGTGG+TGG | - | chr5_1:20252888-20252907 | None:intergenic | 30.0% |
| ! | AACACAAACACAACATGTTT+TGG | + | chr5_1:20252908-20252927 | Msa0722780:CDS | 30.0% |
| GTGAGAGAGTTATTAATGTT+AGG | + | chr5_1:20253195-20253214 | Msa0722780:three_prime_UTR | 30.0% | |
| TGCATATTTCAGTTTCTTCT+TGG | + | chr5_1:20253255-20253274 | Msa0722780:three_prime_UTR | 30.0% | |
| GTAGTGTATTGTAGTATGAA+GGG | + | chr5_1:20253277-20253296 | Msa0722780:three_prime_UTR | 30.0% | |
| TATATAAGCACACACCAACA+AGG | - | chr5_1:20250765-20250784 | None:intergenic | 35.0% | |
| GTTGGTGTGTGCTTATATAA+AGG | + | chr5_1:20250766-20250785 | Msa0722780:five_prime_UTR | 35.0% | |
| GTGCTTATATAAAGGAGGAT+AGG | + | chr5_1:20250774-20250793 | Msa0722780:five_prime_UTR | 35.0% | |
| TCTTGAAACCCTTTAGCTAT+AGG | - | chr5_1:20250831-20250850 | None:intergenic | 35.0% | |
| CATCCATGAAAATTCTCAGT+GGG | + | chr5_1:20251010-20251029 | Msa0722780:CDS | 35.0% | |
| ! | AAACCCACTGAGAATTTTCA+TGG | - | chr5_1:20251016-20251035 | None:intergenic | 35.0% |
| TGTGTATAATATAGGTGGGA+AGG | + | chr5_1:20251273-20251292 | Msa0722780:intron | 35.0% | |
| ATAGATCAAGAGGAAACAGA+AGG | + | chr5_1:20251310-20251329 | Msa0722780:CDS | 35.0% | |
| TCTATGTTATATGCATGCAC+AGG | + | chr5_1:20251451-20251470 | Msa0722780:intron | 35.0% | |
| TATATGCATGCACAGGATAT+TGG | + | chr5_1:20251458-20251477 | Msa0722780:intron | 35.0% | |
| CAATCACCAGTGTTTATTTC+AGG | - | chr5_1:20251525-20251544 | None:intergenic | 35.0% | |
| CTAGCTCATCATCAGTTTAT+GGG | + | chr5_1:20251547-20251566 | Msa0722780:CDS | 35.0% | |
| GACATCTTGAAGAAAAGTTC+AGG | - | chr5_1:20251609-20251628 | None:intergenic | 35.0% | |
| GAGATCTATAACTATGGAGA+AGG | + | chr5_1:20251633-20251652 | Msa0722780:CDS | 35.0% | |
| !!! | ATTTTATGGAGCAGGTTGAT+TGG | + | chr5_1:20251760-20251779 | Msa0722780:intron | 35.0% |
| TCAGTTCTTGTCTGGTATAA+AGG | + | chr5_1:20252034-20252053 | Msa0722780:CDS | 35.0% | |
| !! | AGAGAAGGTGTTGTTCAATT+AGG | + | chr5_1:20252198-20252217 | Msa0722780:CDS | 35.0% |
| ! | TCCCTTTGTTATCTCAAACA+TGG | + | chr5_1:20252245-20252264 | Msa0722780:intron | 35.0% |
| ATGAAACACAACACTGATAC+TGG | - | chr5_1:20252415-20252434 | None:intergenic | 35.0% | |
| GTATCAGTGTTGTGTTTCAT+AGG | + | chr5_1:20252415-20252434 | Msa0722780:intron | 35.0% | |
| GTTGGATAATGAAGAAGAAG+AGG | + | chr5_1:20252992-20253011 | Msa0722780:CDS | 35.0% | |
| !! | AAATGATGTTGATGATACCC+TGG | - | chr5_1:20253065-20253084 | None:intergenic | 35.0% |
| CATTTCCATCAAGATCATCT+TGG | + | chr5_1:20253080-20253099 | Msa0722780:CDS | 35.0% | |
| CATTTCCAAGATGATCTTGA+TGG | - | chr5_1:20253088-20253107 | None:intergenic | 35.0% | |
| CATCAAGATCATCTTGGAAA+TGG | + | chr5_1:20253086-20253105 | Msa0722780:CDS | 35.0% | |
| CTCTCTCACTACAATATGTT+AGG | - | chr5_1:20253184-20253203 | None:intergenic | 35.0% | |
| GGTAGTGTATTGTAGTATGA+AGG | + | chr5_1:20253276-20253295 | Msa0722780:three_prime_UTR | 35.0% | |
| CACACATTTGCATGGAATAA+TGG | - | chr5_1:20253316-20253335 | None:intergenic | 35.0% | |
| ACAATCTTCACACATTTGCA+TGG | - | chr5_1:20253324-20253343 | None:intergenic | 35.0% | |
| CCAAGTACAATGTAGTAGAT+TGG | - | chr5_1:20253394-20253413 | None:intergenic | 35.0% | |
| CCAATCTACTACATTGTACT+TGG | + | chr5_1:20253391-20253410 | Msa0722780:three_prime_UTR | 35.0% | |
| GGTGTGTGCTTATATAAAGG+AGG | + | chr5_1:20250769-20250788 | Msa0722780:five_prime_UTR | 40.0% | |
| GGAAGAACATCTTACTCCAT+TGG | + | chr5_1:20250947-20250966 | Msa0722780:CDS | 40.0% | |
| AGAAGATGAGTAACAGCCAA+TGG | - | chr5_1:20250966-20250985 | None:intergenic | 40.0% | |
| GCATCCATGAAAATTCTCAG+TGG | + | chr5_1:20251009-20251028 | Msa0722780:CDS | 40.0% | |
| !!! | AGTGGGTTTATGCTGTTTTC+TGG | + | chr5_1:20251027-20251046 | Msa0722780:CDS | 40.0% |
| CTATGATCTTCTTACTTGGG+TGG | - | chr5_1:20251077-20251096 | None:intergenic | 40.0% | |
| TAATATAGGTGGGAAGGACA+AGG | + | chr5_1:20251279-20251298 | Msa0722780:intron | 40.0% | |
| AATATAGGTGGGAAGGACAA+GGG | + | chr5_1:20251280-20251299 | Msa0722780:intron | 40.0% | |
| CAAGAGGAAACAGAAGGAAT+TGG | + | chr5_1:20251316-20251335 | Msa0722780:CDS | 40.0% | |
| !! | CATGCACAGGATATTGGTTT+GGG | + | chr5_1:20251464-20251483 | Msa0722780:intron | 40.0% |
| !! | AGGATATTGGTTTGGGAAGA+TGG | + | chr5_1:20251471-20251490 | Msa0722780:intron | 40.0% |
| ! | CCATAAACTGATGATGAGCT+AGG | - | chr5_1:20251549-20251568 | None:intergenic | 40.0% |
| CCTAGCTCATCATCAGTTTA+TGG | + | chr5_1:20251546-20251565 | Msa0722780:CDS | 40.0% | |
| !! | AAAGTTCAGGTTGAAGACCT+TGG | - | chr5_1:20251596-20251615 | None:intergenic | 40.0% |
| ! | GATTTCTTTTACAGCACCCT+AGG | + | chr5_1:20252000-20252019 | Msa0722780:intron | 40.0% |
| ACCTTCTCTAACAGCAATGA+GGG | - | chr5_1:20252187-20252206 | None:intergenic | 40.0% | |
| TCAATTAGGAGCTGTTCACA+AGG | + | chr5_1:20252212-20252231 | Msa0722780:CDS | 40.0% | |
| GATTGAAGACTTGAGCTATG+TGG | + | chr5_1:20252617-20252636 | Msa0722780:CDS | 40.0% | |
| ! | AGCTACATTGAAAGCATACC+AGG | + | chr5_1:20252660-20252679 | Msa0722780:CDS | 40.0% |
| ! | AAGGGTATGCTGATGATGAT+GGG | - | chr5_1:20252701-20252720 | None:intergenic | 40.0% |
| ! | TAAGGGTATGCTGATGATGA+TGG | - | chr5_1:20252702-20252721 | None:intergenic | 40.0% |
| TCATCATCAGCATACCCTTA+TGG | + | chr5_1:20252702-20252721 | Msa0722780:CDS | 40.0% | |
| ! | AAGTTGTGCCATTGTTCTGA+TGG | - | chr5_1:20252732-20252751 | None:intergenic | 40.0% |
| GAACAATGGCACAACTTTCA+AGG | + | chr5_1:20252735-20252754 | Msa0722780:CDS | 40.0% | |
| AATTGATCACCTTGATGCTG+TGG | - | chr5_1:20252771-20252790 | None:intergenic | 40.0% | |
| GCTTCAAGACTACTCATTGA+TGG | - | chr5_1:20252837-20252856 | None:intergenic | 40.0% | |
| AACACAACAACAACGAGCAT+TGG | + | chr5_1:20252932-20252951 | Msa0722780:CDS | 40.0% | |
| !! | CAACGAGCATTGGAATTTAC+AGG | + | chr5_1:20252942-20252961 | Msa0722780:CDS | 40.0% |
| AAAAGTCGCGAAAGAAGAGT+TGG | + | chr5_1:20252974-20252993 | Msa0722780:CDS | 40.0% | |
| AGACCTGAACAACTTGATGT+TGG | + | chr5_1:20253020-20253039 | Msa0722780:CDS | 40.0% | |
| ! | TCACCAACATCAAGTTGTTC+AGG | - | chr5_1:20253026-20253045 | None:intergenic | 40.0% |
| AGAGTAGTAGTTCCATACCA+GGG | + | chr5_1:20253045-20253064 | Msa0722780:CDS | 40.0% | |
| !! | ATGTTGATGATACCCTGGTA+TGG | - | chr5_1:20253060-20253079 | None:intergenic | 40.0% |
| !!! | GGGTTTATGCTGTTTTCTGG+AGG | + | chr5_1:20251030-20251049 | Msa0722780:CDS | 45.0% |
| GATCTTCTTACTTGGGTGGA+GGG | - | chr5_1:20251073-20251092 | None:intergenic | 45.0% | |
| TGATCTTCTTACTTGGGTGG+AGG | - | chr5_1:20251074-20251093 | None:intergenic | 45.0% | |
| ATATAGGTGGGAAGGACAAG+GGG | + | chr5_1:20251281-20251300 | Msa0722780:intron | 45.0% | |
| GGGGCATATGATAGATCAAG+AGG | + | chr5_1:20251300-20251319 | Msa0722780:CDS | 45.0% | |
| ! | GCATGCACAGGATATTGGTT+TGG | + | chr5_1:20251463-20251482 | Msa0722780:intron | 45.0% |
| GCAGCACCTGAAATAAACAC+TGG | + | chr5_1:20251516-20251535 | Msa0722780:CDS | 45.0% | |
| !! | TCAGGTTGAAGACCTTGGTA+AGG | - | chr5_1:20251591-20251610 | None:intergenic | 45.0% |
| CTGCAGATCATAGTCACAAG+TGG | + | chr5_1:20251791-20251810 | Msa0722780:CDS | 45.0% | |
| ! | TTTTACAGCACCCTAGGACT+TGG | + | chr5_1:20252006-20252025 | Msa0722780:intron | 45.0% |
| TTTACAGCACCCTAGGACTT+GGG | + | chr5_1:20252007-20252026 | Msa0722780:intron | 45.0% | |
| CACCTTCTCTAACAGCAATG+AGG | - | chr5_1:20252188-20252207 | None:intergenic | 45.0% | |
| AACCGCAACCATGTTGCAAT+CGG | + | chr5_1:20252452-20252471 | Msa0722780:intron | 45.0% | |
| ATGTCTCTCCGATTGCAACA+TGG | - | chr5_1:20252463-20252482 | None:intergenic | 45.0% | |
| TCATGGATCAGTCGCATTCT+CGG | + | chr5_1:20252497-20252516 | Msa0722780:intron | 45.0% | |
| !! | TATGCTGATGATGATGGGTG+TGG | - | chr5_1:20252696-20252715 | None:intergenic | 45.0% |
| TCATCAGCATACCCTTATGG+TGG | + | chr5_1:20252705-20252724 | Msa0722780:CDS | 45.0% | |
| ! | GTTCTGATGGACCACCATAA+GGG | - | chr5_1:20252719-20252738 | None:intergenic | 45.0% |
| ! | TGTTCTGATGGACCACCATA+AGG | - | chr5_1:20252720-20252739 | None:intergenic | 45.0% |
| ATGGTGGTCCATCAGAACAA+TGG | + | chr5_1:20252721-20252740 | Msa0722780:CDS | 45.0% | |
| ! | TGTTGTGGTGGAACAACTGA+AGG | - | chr5_1:20252876-20252895 | None:intergenic | 45.0% |
| GAGAGTAGTAGTTCCATACC+AGG | + | chr5_1:20253044-20253063 | Msa0722780:CDS | 45.0% | |
| TTGCATCCTTAAACAGCAGC+AGG | - | chr5_1:20253437-20253456 | None:intergenic | 45.0% | |
| !!! | TAAGAATTTTATTTTATATA+TGG | + | chr5_1:20250926-20250945 | Msa0722780:exon | 5.0% |
| TTGGGTGGAGGGTAGTTTCT+AGG | - | chr5_1:20251062-20251081 | None:intergenic | 50.0% | |
| ACTGAGCTTCCCAAGTCCTA+GGG | - | chr5_1:20252019-20252038 | None:intergenic | 50.0% | |
| AACTGAGCTTCCCAAGTCCT+AGG | - | chr5_1:20252020-20252039 | None:intergenic | 50.0% | |
| TGGGAAGCTCAGTTCTTGTC+TGG | + | chr5_1:20252026-20252045 | Msa0722780:CDS | 50.0% | |
| ! | GCCCTCATTGCTGTTAGAGA+AGG | + | chr5_1:20252183-20252202 | Msa0722780:CDS | 50.0% |
| CTCCGATTGCAACATGGTTG+CGG | - | chr5_1:20252457-20252476 | None:intergenic | 50.0% | |
| AGCATTGCACCACAGCATCA+AGG | + | chr5_1:20252759-20252778 | Msa0722780:CDS | 50.0% | |
| ! | CTAGCTCCTGCTGCTGTTTA+AGG | + | chr5_1:20253428-20253447 | Msa0722780:three_prime_UTR | 50.0% |
| ACAGCAGCAGGAGCTAGCAT+TGG | - | chr5_1:20253425-20253444 | None:intergenic | 55.0% | |
| GGGTGTGGAAGTAGCACTCC+TGG | - | chr5_1:20252681-20252700 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 20250730 | 20253537 | 20250730 | ID=Msa0722780;Name=Msa0722780 |
| chr5_1 | mRNA | 20250730 | 20253537 | 20250730 | ID=Msa0722780-mRNA-1;Parent=Msa0722780;Name=Msa0722780-mRNA-1;_AED=0.16;_eAED=0.16;_QI=215|1|1|1|1|1|7|404|364 |
| chr5_1 | exon | 20250730 | 20251081 | 20250730 | ID=Msa0722780-mRNA-1:exon:7660;Parent=Msa0722780-mRNA-1 |
| chr5_1 | exon | 20251287 | 20251337 | 20251287 | ID=Msa0722780-mRNA-1:exon:7661;Parent=Msa0722780-mRNA-1 |
| chr5_1 | exon | 20251473 | 20251655 | 20251473 | ID=Msa0722780-mRNA-1:exon:7662;Parent=Msa0722780-mRNA-1 |
| chr5_1 | exon | 20251774 | 20251879 | 20251774 | ID=Msa0722780-mRNA-1:exon:7663;Parent=Msa0722780-mRNA-1 |
| chr5_1 | exon | 20252014 | 20252055 | 20252014 | ID=Msa0722780-mRNA-1:exon:7664;Parent=Msa0722780-mRNA-1 |
| chr5_1 | exon | 20252177 | 20252233 | 20252177 | ID=Msa0722780-mRNA-1:exon:7665;Parent=Msa0722780-mRNA-1 |
| chr5_1 | exon | 20252615 | 20253537 | 20252615 | ID=Msa0722780-mRNA-1:exon:7666;Parent=Msa0722780-mRNA-1 |
| chr5_1 | five_prime_UTR | 20250730 | 20250944 | 20250730 | ID=Msa0722780-mRNA-1:five_prime_utr;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20250945 | 20251081 | 20250945 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20251287 | 20251337 | 20251287 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20251473 | 20251655 | 20251473 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20251774 | 20251879 | 20251774 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20252014 | 20252055 | 20252014 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20252177 | 20252233 | 20252177 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | CDS | 20252615 | 20253133 | 20252615 | ID=Msa0722780-mRNA-1:cds;Parent=Msa0722780-mRNA-1 |
| chr5_1 | three_prime_UTR | 20253134 | 20253537 | 20253134 | ID=Msa0722780-mRNA-1:three_prime_utr;Parent=Msa0722780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
